Publications

isolation of l-forms by blood culture.	culture media for the isolation of bacterial l-forms from the blood were studied. the most successful media had an osmolality of more than 1100 mosm/kg and this appeared to be a critical factor in determining success.	1976	789403
beta-lactamases and resistance to penicillins and cephalosporins in serratia marcescens.	strains of serratia marcescens fall into one of two groups with respect to their resistance to to beta-lactum antibiotics. most strains are highly resistant to cephalosporins but are significantly more susceptible to ampicillin and carbenicillin, whereas other strains are highly resistant to both penicillins and cephalosporins. strains in the former category produce small amounts of an inducible cephalosporinase, which appears to be chromosomally mediated. strains in the latter class also elabo ...	1976	789784
isolation of rough mutants of klebsiella aerogenes and their synthesis of polysaccharides.	two mutants which lacked both capsular and lipopolysaccharide o-antigen polysaccharides were isolated from klebsiella aerogenes serotype 2 by phage selection; these were designated rough mutants. the polysaccharide fractions solubilized by partial acid hydrolysis of the lipopolysaccharide from both the wild type and mutants were chromatographed on sephadex g-50. analysis of the fractions obtained confirmed that the rough mutants lacked the galactan portion of the molecule, which is analogous to ...	1976	789814
the urographic findings in acute pyelonephritis: non-obstructive hydronephrosis.	excretory urography was performed on 4 patients early in the course of acute pyelonephritis. these studies showed dilatation of the involved collecting system without obstruction. the dilatation resolved after therapy in 3 patients and was not present on a previous excretory urogram in the fourth. although previous studies have shown that the excretory urogram is usually normal in cases of acute pyleonephritis these cases demonstrate that this disease can produce dilatation of the collecting sys ...	1976	789916
[control of cross infection in intensive care units].	quantitative determinations of antibiotic susceptibility established that infections occurring in intensive care units were predominantly exogenous cross infections. the most frequently isolated strains were: klebsiella, enterobacteriaceae, serratia marcescens and proteus mirabilis. the majority of the strains showed multiple resistance. despite adequate antibiotic therapy a number of patients died of the infection. many of the methods of disinfection were found to be inadequate, especially as r ...	1976	790370
[disinfection of the inspired and expired air during artificial respiration with humified gases].	the water in the nebulizer of respirators is a potential source of infection for patients receiving artificial respiration. interposition of a special equipment that will produce ozone-free ultraviolet radiation will prevent infection of the patient by the inspired humidified air, even if the bacterial count in the nebulizer fluid is very high. interposition of the same equipment on the expiratory side is also recommended to minimize the risk of infection of the environment by bacteria in the ex ...	1976	790371
[polyallergic (concomitant) reactions to bacterial allergens in patients with pre-asthmatic conditions and infectious-allergic bronchial asthma].		1976	790594
five-day course of antibacterials for uncomplicated urinary infections.	fifty-six patients with uncomplicated urinary infections were treated with a five-day course of antimicrobials. fifty-four patients had sterile urine two weeks after termination of the drugs. over a two-year period 8 patients were found to become reinfected.	1976	790745
[comparative studies with the rapid kcn-test for enterobacteriaceae according to cahan, sechter and ohad (author's transl)].	581 strains of enterobacteriaceae (184 of salmonella, 100 of shigella, 31 of citrobacter freundii, 61 of escherichia coli, 40 of klebsiella, 165 of proteus) were investigated in comparison with the kcn-test according to møller and with the rapid kcn-test according to cahan, sechter and ohad. there was found that the results obtained with both methods were identical to 99 percent. in both tests there could be observed that inexplicable differences occur only in two strains of proteus mirabilis. t ...	1976	790855
inositol as a selective substrate for the growth of klebsiellae and serratiae.	a synthetic basal salts medium containing inositol as the sole source for carbon and energy and bromothymol blue as ph indicator, selectively supports the growth of klebsiella pneumoniae and serratia spp. on this medium incubated at 37 degrees c for 24 hr, all cultures of kl. pneumoniae, serratia liquefaciens and s. marcescens form yellow colonies. the medium may be used selectively for the primary isolation of the aforementioned bacteria from faeces and as a differential medium for their isolat ...	1976	790856
human oral defenses against gram-negative rods.	gram-negative rods are unusual pharyngeal isolates in normal man, which suggests the presence of effective oral defense mechanisms against these organisms. to understand this protection better, we studied the elimination of gram-negative rods from the mouth after gargle challenge. suspensions of escherichia coli, klebsiella pneumoniae, and proteus mirabilis (108 organisms per ml) were gargled by normal volunteers, and pharyngeal swabs for serial quantitative cultures were taken for 3 hours. init ...	1976	791034
susceptibility of acinetobacter calcoaceticus var. anitratus (herellea vaginicola) to minocycline.	the in vitro activity of minocycline against acinetobacter calcoaceticus var. anitratus (herellea vaginicola) was examined. all strains of a. anitratus were inhibited by minocycline at concentrations of 0.4 mug/ml or less. the strains studied were considerably more susceptible to minocycline than tetracycline. no difference between the susceptibility to minocycline and tetracycline was seen with escherichia coli, klebsiella pneumoniae, enterobacter and proteus sp. a regression line of the log mi ...	1976	791084
r factor-mediated antibiotic resistance in serratia marcescens.	nineteen of 39 multiresistant strains of serratia marcescens isolated from clinical sources transferred antibiotic resistance to escherichia coli or klebsiella pneumoniae recipients. marcesins and/or phage prevented effective resistance transfer to e. coli and attempts to select marcescin-resistant mutants of the e. coli recipient strain were unsuccessful. transfer of resistance was demonstrated for all drugs tested except nalidixic acid. approximately 90% of donors resistant to tobramycin, ampi ...	1976	791085
in vitro synergistic effect of netilmicin, a new aminoglycoside antibiotic.	the combination of netilmicin (sch 20569) with carbenicillin, cefazolin, chloramphenicol, and clindamycin was evaluated by in vitro tests against 91 clinical isolates of pseudomonas, klebsiella, and escherichia coli. combinations were considered to show synergy if there was a fourfold or greater reduction in minimal inhibitory concentration values of both antibiotics when combined. synergy of netilmicin with carbenicillin could be demonstrated against 21 of 46 pseudomonas isolates. comparison of ...	1976	791104
oxygen transfer rate, respiration and yields in batch and chemostat cultures of klebsiella aerogenes.	the effect was studied of oxygen supply on the changes in total and specific rate of oxygen consumption by the cells, oxygen transfer rate, saturation concentrations of dissolved oxygen and the yields of batch and continuous cultivations. experiments were done on the microorganism klebsiella aerogenes ccm 2318 growing on synthetic glucose medium. continuous cultivations were carried out at dilution rates of 0.96 and 0.178 h-1. the rate of oxygen transfer was determined by the sulphite method and ...	1976	791773
in vitro growth inhibition of mastitis-causing coliform bacteria by bovine apo-lactoferrin and reversal of inhibition by citrate and high concentrations of apo-lactoferin.	bovine apo-lactoferrin (apo-lf) was added to in vitro cultures of eight strains of coliform bacteria associated with bovine mastitis. as little as 0.02 mg of apo-lf per ml resulted in marked inhibition of growth of all coliforms. growth inhibition was lost if saturated lf or iron plus apo-lf was added to the synthetic medium. the inhibition of growth increased as the concentration of apo-lf increased from 0.02 to 0.2 mg/ml for klebsiella pneumoniae (oardc-a1), klebsiella spp. (k1-21), and aeroba ...	1976	791866
use of bacteriophage mu to isolate deletions in the his-nif region of klebsiella pneumoniae.	klebsiella pneumoniae m5a1 is naturally resistant to infection by bacteriophage mu. mutants of k. pneumoniae sensitive to mu infection were isolated and found to support both lytic and lysogenic development of mu. k. pneumoniae lysogens containing a heat-inducible mu prophage integrated in his were isolated. strains carrying deletions extending from his into nif were obtained after heat treatment of these lysogens. such deletions should be useful for determining the map order and cistronic organ ...	1976	791929
clinical evaluation of the minitek differential system for identification of enterobacteriaceae.	forty-one stock organisms and 581 fresh clinical isolates were used in comparing the minitek system to conventional tubed media to determine if this system is feasible and accurate for a high-volume clinical microbiology laboratory. in addition to comparison with tubed media, the following parameters were tested: (i) reproducibility of disks, (ii) the effect of variation in inoculum size, (iii) the effect of the age of culture, and (iv) the effect of predispensing disks. a total of 5,947 disks w ...	1976	791964
toxigenic bacterial diarrhea: nursery outbreak involving multiple bacterial strains.	an outbreak of watery diarrhea occurred in 14 of 15 infants in a special care unit over a four-day period. using the cho cell assay for enterotoxin, we found that 11 of these patients had toxigenic bacteria in their stools. these bacteria comprised nine different serotypes of three species of organisms: escherichia coli, klebsiella, and citrobacter. none of the three serotypes of e. coli were classic enteropathogenic serotypes. rectal swab specimens from all 15 infants were examined for the pres ...	1976	792408
a study of bacterial aerosols at a wastewater irrigation site.		1976	792471
effect of cephalothin on growth patterns of micro-organisms.	isolates of staphylococcus aureus, escherichia coli. klebsiella pneumoniae and proteus mirabilis were incubated in the presence of inhibitory concentrations of cephalothin. after destruction of the antibiotic, there was a lag phase before s. aureus began to proliferate again. when similar experiments were conducted with e. coli, k. pneumoniae, and p. mirabilis, no lag phase was observed. this data suggests that the inhibitory activity of cephalosporins may be different for gram-positive cocci an ...	1976	792494
effect of capsular polysaccharide of klebsiella pneumoniae on host resistance to bacterial infections. ii. effects on peritoneal leukocytes of normal mice and mice infected with virulent salmonella enteritidis.	in normal mice, the total count of peritoneal leukocytes was markedly decreased after intraperitoneal (i.p.) injection of the capsular polysaccharide of klebsiella pneumoniae (cps-k) depending on the dosage injected. this decrease was mainly due to the depletion of macrophages, and a decrease in the number of lymphocytes occurred to a lesser extent. cps-k in relatively smaller doses mobilized polymorphonuclear neutrophilic leukocytes (pmn) into the peritoneal fluid but it decreased them transien ...	1976	792533
cadmium and zinc sensitivity and tolerance in klebsiella (aerobacter) aerogenes.	the resistance of strains of k. aerogenes ncib 418 'trained' to cd2+ or zn2+ in liquid medium is graded to the training concentration. training to cd2+ increased the sensitivity to zn2+ but training to zn2+ reduced the sensitivity to cd2+. the trained organisms, particularly those trained to cd2+, grow slowly in medium containing the metals and the growth rates after 20 and 200 subcultures were not significantly different. the survival of untrained organisms on cd2+-agar decreased progressively ...	1976	792633
the butanol-soluble proteins of klebsiella aerogenes.	membranes of klebsiella aerogenes were extracted with acid butanol at ph 4.2 and the solubilized proteins were fractionated by precipitation at 0 degrees c and -20 degrees c. the -20 degrees c precipitate was examined by polyacrylamide gel electrophoresis in sodium dodecyl sulphate. several enzyme activities associated with polysaccharide biosynthesis have been identified in the crude -20 degrees c precipitate and an attempt was made to separate the enzymes from other protein. the role of certai ...	1976	792634
oral manifestations of crohn's disease: studies in the pathogenesis.		1976	792883
[effect of hyperbaric oxygen on aerobic bacteria].	the authors investigated the growth of 8 species of pathogenic aerobic bacteria after treatment with hyperbaric oxygen. within 24 hours the cultures were exposed three times for 2 hours to an absolute pressure of 3 atmospheres of oxygen at 37 degrees c. in the fluid cultures as well as in the controls the inhibitory effect of oxygen was assessed in percent by means of the densitometric method. from these in vivo experiments the authors conclude that hyperbaric treatment of superficial pyogenic e ...	1976	793215
enterobacteriaceae in the jejunal microflora prevalence and relationship to biochemical and histological evaluations in healthy colombian men.	when 23 healthy native southwestern colombian men were studied to determine the prevalence of enterobacteriaceae in the jejunal microflora in a sample of thoroughly evaluated tropical inhabitants who were normal by physical examination, chest x-ray, and medical history, 14 of the 23 proved to be enterobacteriaceae-positive, with counts of 10(3) to 10(9) per milliliter of jejunal aspirate. thirteen had escherichia coli, and the fourteenth had klebsiella pneumoniae. four had a second species of en ...	1976	793372
radiologic features of gram-negative pneumonias in the neutropenic patient.	while gram-negative pneumonia is a frequent complication in immunosuppressed patients with malignant disease, the diagnosis is difficult to establish. clinical and radiographic study of 195 episodes of pneumonia in 175 consecutive patients was undertaken. chest radiographs were negative in 19% of the episodes, probably due to absence of alveolar inflammatory cell infiltrate. radiographic patterns were classified as either alveolar or mixed alveolar and interstitial; no case of interstitial patte ...	1976	793427
[experimental study of the chemotherapeutic activity of gentamicin sulfate].	antibacterial activity of gentamycin sulfate was studied in vitro and in treatment of albino mice with experimental infections. gentamycin was superior to kanamycin with respect to its antibacterial effect against clinical strains of staphylococcus, coli bacteria, proteus and ps. aeruginosa. high efficiency of gentamycin was found with respect to acute and chronic staphylococcal infection, acute proteus and coli sepsis. the antibiotic was characterized by low ld50, high chemotherapeutic index, r ...	1976	793517
evaluation of double violet agar in the isolation of klebsiella pneumoniae from river water.	a field evaluation of double violet agar for the isolation and presumptive identification of klebsiella pneumoniae from water has been performed. water from the north oconee river, clarke county, ga., was cultured for presence of klebsiellae using the membrane filter technique. colonies were presumptively identified as k. pneumoniae in the basis of their appearance on double violet agar. such identifications were evaluated using appropriate biochemical tests. once investigators have become famil ...	1976	793520
[clinical significance of sperm pathogens in fertility disorders].	the reasons for systematic microbial examinations of human ejaculates are discussed. the exact establishment of an association between bacteria isolated from human ejaculates and infertility is very difficult. there are several hints for such an association in the case of different micoplasmas though there is no clear-cut evidence. a physical association between these microorganisms and spermatozoa, which was recently observed, possibly could help to facilitate the diagnosis.	1976	793968
simplified scheme for identification of prompt lactose-fermenting members of the enterobacteriaceae.	a brief, simplified scheme involving the spot indole test and colonial morphology was evaluated for genus level identification of prompt lactose-fermenting (plf) members of the enterobacteriaceae. one hundred and ninety-four consecutive, clinically important plf gram-negative rods isolated in a clinical microbiology laboratory were identified by this simplified scheme, as well as by standard biochemical tests, and the api 20e (analytab products, inc., plainview, n.y.) system. in the simplified s ...	1976	794078
klebsiella and enterobacter organisms isolated from horses.	an account is given of k. pneumoniae capsule types occurring in horses, with particular reference to strains originating from the genital tract in the mare and the external genitalia of the stallion. a survey of the prevalence of k. pneumoniae and e. aerogenes strains in the preputial flora of healthy stallions is described. the majority of horses were found to be carriers of these organisms. the cultural characteristics of these preputial strains are described and compared with those of k. pneu ...	1976	794407
role of b-lymphocytes in nonspecific resistance to klebsiella pneumoniae infection of endotoxin-treated mice.	lipopolysaccharides (lps) of gram-negative bacilli are known to protect mice against unrelated bacterial infections and to be nonspecific mitogens of murine bone marrow-derived (b-) lymphocytes. for assessment of the role of these cells in the mechanism of lps-induced resistance to infection with klebsielia, various nontoxic mitogens were assayed. in contrast to lps or lipid a, the nontoxic mitogens did not protect mice. experiments were also performed with lps in nude mice and in mice treated w ...	1976	794421
[rhinoscleroma with sinuso-orbitary invasion].	the authors present the case of an 11-year-old child with a slowly progressive tumoral invasion resulting in bilateral nasal obstruction, massive facial deformation, and an exophthalmos of the left eye. there was no impairment of the child's general health. the histological investigation revealed the presence of mikulicz' cells and von frisch's bacilli. the resultant diagnosis was rhinoscleroma.	1976	794784
biochemistry and genetics of nitrate reductase in bacteria.		1976	795276
[etiology of pyelonephritis in pregnant and parturient women].		1976	795316
isolation of klebsiella pneumoniae from lake water.	the question of the importance of aquatic borne klebsiella pneumoniae to public health has been argued as the organism has appeared often in both polluted effluents and oligotrophic systems. using a selective medium, double violet agar, which also differentiates k. pneumoniae from other organisms found in aquatic systems, we sampled water from three ponds and a large man-made lake, over a 3-month period. results indicate that k. pneumoniae can be isolated consistently and in high numbers from eu ...	1976	795534
presumptive identification of klebsiella pneumoniae on m-fc medium.	klebsiella pneumoniae is a human and animal pathogen frequently encountered in the environment. the membrane-filtration method utilizing m-fc medium for the detection of fecal coliforms yields a colony type easily distinguishable as k. pneumoniae. these colonies appear as atypical light blue, nucleated, mucoid colonies differing considerably from the typical dark blue fecal coliform colonies.	1976	795536
the lactose system of klebsiella aerogenes v9a. 5. lac-permease defective mutants of two klebsiella lac plasmids and their apparent reversion to wild type.		1976	795725
immunofluorescence detection of nitrogenase proteins in whole cells.	fluorescent antibodies (fa) prepared against the mo-fe and fe proteins of nitrogenase from klebsiella pneumoniae m5ai were used to detect these protein components in toluene-treated whole cells that were actively reducing acetylene. the fa were highly specific, staining only nitrogenase component proteins originating from klebsiella. cross-reactions between the fa and purified nitrogenase proteins from other dinitrogen-fixing micro-organisms did not occur, except in the case of bacillus polymyxa ...	1976	796412
[comparative results of cephacetrile with cefazolin in complicated urinary tract infections by means of double-blind trials (author's transl)].	in order to elucidate the usefulness of cephacetrile (cec), comparative trials with cefazolin (cez) were carried out in the patients with complicated infections of the urinary tract, and the following results were obtained: 1. there were no statistically significant differences or tendencies observed between the groups given cec and cez in either of the global effects, bacteriological effects, rate of superinfection, relapse, relapse with bacterial alternation, drug usefulness, rate of improveme ...	1976	796485
studies on the effect of phytagglutinins on some members of enterobacteriaceae.	extracts of twenty seeds were tested against twenty six organisms belonging to klebsiella, proteus, salmonella, shigella and cholera species. extracts of eleven seeds showed agglutinating activity against twenty four various organism. s. typhi and kl. aerogenes did not react against any lectin. on the basis of the results it was possible to differentiate various shigella and salmonella organisms by various seed extracts. different isolates of cholera organisms of same serotype and phage type sho ...	1976	796502
[role of conventionally pathogenic/intestinal bacteria in surgical practice (literature survey)].		1976	796557
the mutagenicity of cyclamates and their metabolites.		1976	796692
[rhinoscleroma in an immigrant].	a patient with rhinoscleroma was treated in our department. as far as we know it is the first importcase published in belgium. rhinoscleroma is an infectious disease caused by the klebsiella rhinoscleromatising. it is a chronic granulomatous process with subsequent stenosis and scarring. the nose and larynx are the most frequent localisations. the therapy consists of antibiotics in an early stage and is only palliative in the latter ones.	1976	797230
[systematic study of pharyngeal and nasal bacteria in children affected by relapsing diseases of respiratory organs (author's transl)].	the research was conducted on 191 nasal and pharyngeal swabs from children aged between two and ten years, affected by relapsing diseases of their respiratory organs. the aim has been that of isolating those bacteria whose action cause the most frequent respiratory diseases. they are: haemophilus influenzae, staphylococcus c+, klebsiella pneumoniae, pseudomonas and other gram-negative bacteria. the isolated bacteria were submitted to sensitivity tests and to the normally used antibiotics. from t ...	1976	797320
[comparative analysis between qualitative and quantitative features of orofaringeal and tracheo-bronchial bacterial flora in normal subject (author's transl)].	in 28 subjects surely without tracheo-bronchial phlogosis it had been observed that the trachea and the bronchi are mostly sterile, only rarely there is a normal bacterial flora. in the mouth and in the pharynx there is instead, almost always, normal bacterial flora and only exceptionally potentially pathogenous strains at a concentration inferior to 10(7).	1976	797321
[gentamicin resistance and the r factor in the strains isolated from the urinary tract (author's transl)].		1976	798057
[relationship between the ultrastructure and chemical composition of the capsula in klebsiella rhinoscleromatis].		1976	798151
effect of dietary oxytetracycline on microorganisms in turkey feces.	thirty-six two-day old male turkey poults were divided into three groups and fed three levels of oxytetracycline; 0, 110 or 440 mg. per kg. of ration, respectively, for nine weeks. the poults were given an oral spectinomycin plus vitamin treatment at the hatchery but were not fed before the start of the experiment. defecated feces from each group were sampled at weekly intervals and analyzed for bacteriological content, especially for members of the enterobacteriaceae. total aerobic counts of 3 ...	1976	798178
[experimental pyelonephritis in rats: value in the comparison of antibiotics (author's transl)].	the author discusses the conditions which an experimental pyelonephritis must fulfill to provide therapeutic results which will allow the assessment of new therapeutic agents. he subjects the chosen model to the therapeutic effect of four antibiotics and three antimicrobial drugs. a numeric value is given to the bacteriuria recession slope observed during the experiment. a comparison of the results being obtained with the bacteriological and pharmacological data normally evaluated, the author de ...	1976	798516
some clinical and radiological aspects of the toxic enteritis in suckling age.		1976	799607
cutaneous protothecosis in a patient receiving immunosuppressive therapy.	a 30-year-old recipient of a renal transplant, who was receiving immunosuppressive therapy, developed cutaneous abscesses from which an organism of the genus prototheca was cultured. the patient died of klebsiella septicemia. at the time of death, pure cultures of prototheca and candida organisms were grown from separate skin lesions. prototheca is a genus of achloric algae. cultural, microscopical, and staining characteristics separate these organisms from the yeast and fungi. cutaneous prototh ...	1976	782362
[immobilized antibodies: a possible technic for the epidemiological study of antibiotic resistant bacteria].	an antibody specific to a cephalosporinase responsible for the resistance of bacteria to cephalosporines has been immobilized on agarose beads by the iminocarbonate method. the resin thus obtained has been studied at a biospecific point of view. its high thermostability is also demonstrated. this biospecific resin allows the performance of epidemiologic experiments of bacteria resistant to beta lactamines.	1976	782563
[localization in cells of neisseria perflava and klebsiella pneumoniae of antigens in common (cross-reacting) with tissues of the human bronchopulmonary apparatus].	a study was made of localization in the cells of neisseria perflava and klebsiella pneumoniae of antigens common with the tissues of the human broncho-pulmonary apparatus. a study was made of cross reactions of some structures of neisseria perflava and klebsiella pneumoniae cells (capsule, walls, cytoplasmic structure fraction, hyaloplasm) in the complement fixation test with antilung sera. antigens cross reacting with the antilung sera were localized not only in the surface structures (walls) o ...	1976	782606
co-trimoxazole in prevention of bacteriuria after prostatectomy.	in a prospective, double-blind trial prophylactic cotrimoxazole produced a highly significant reduction in the incidence of bacteriuria after prostatectomy. only two out of 38 patients who received the drug developed bacteriuria compared with 19 out of 36 patients on placebo. klebsiella-enterobacter spp and coagulase-negative staphylococci were responsible for most infections. although co-trimoxazole prophylaxis is obviously effective, widespread use might increase the incidence of bacterial res ...	1976	782651
[treatment of peritonitis within the scope septic shock with antibiotics].		1976	782801
[sisomicin versus gentamicin. a comparison of antibacterial and pharmacokinetic properties (author's transl)].	in a comparison of the antibacterial in-vitro activity of sisomicin, gentamicin, and tobramycine, sisomicin showed a higher activity against e. coli, indole-positive proteus spp. and organisms of the klebsiella-enterobacter-serratia group, whereas tobramycine was superior against pseudomonas spp. however, the differences in activity between sisomicin and gentamicin were only within one step of dilution which is hardly sufficient to guarantee a basic superiority of one or the other preparation fo ...	1976	782808
[evaluation of diagnostic parameters in chronic recurrent urinary tract infection in children. iii. pyuria, hematuria, and proteinuria (author's transl)].	pyuria, proteinuria and haematuria have been shown to be unreliable parameters for the diagnosis of bacteriuria in 171 children with 455 recurrences of chronic urinary tract infection. bacteriuria caused haematuria in 5%, proteinuria in 18% and even pyuria in only 47% of all instances. pyuria has been also found in 11% of sterile urines. the incidence of pyuria increases with the age of the children and depends on the type of the organisms, but the latter was true mainly in children with obstruc ...	1976	782812
infections in the surgical departments of a teaching hospital in jerusalem.	surveillance of hospital-associated infections at the hadassah university hospital has been carried out since 1970. criteria for infection were defined and were monitored by trained nurse-epidemiologists, with the cooperation of the surgical staff. this report deals with the results of a prospective study of infections present on admission, and those acquired during hospitalization, in six surgical departments between january 1972 and june 1973. of the 7,339 patients discharged, 7.5% acquired an ...	1976	783080
microbial metabolism of amino alcohols. ethanolamine catabolism mediated by coenzyme b12-dependent ethanolamine ammonia-lyase in escherichia coli and klebsiella aerogenes.		1976	784902
the apparent atp requirement for nitrogen fixation in growing klebsiella pneumoniae.	the apparent atp requirement for n2 fixation in klebsiella pneumoniae was high (the atp/n2 molar ratio was 29 when estimated in anaerobic glucose-limited chemostat cultures) compared with that determined previously in azotobacter chroococcum and in clostridium pasteurianum. the high value was probably not due to unfavourable temperature, phosphate concentration or ph. the apparent atp requirement for n2 fixation was probably no lower in 02-limited chemostat cultures than in anaerobic glucose-lim ...	1976	784906
gram-negative bacillary meningitis therapy. polyradiculitis following intralumbar aminoglycoside administration.	gram-negative bacillary meningitis is a serious threat to patients with head trauma or altered immune systems, or those who have had neurosurgical procedures. the aminoglycoside antibiotics administered systemically and into the cerebrospinal fluid (csf) have proved useful in the treatment of these infections. intralumbar and parenteral gentamicin sulfate and tobramycin sulfate were administered to an acromegalic woman with klebsiella meningitis. she had objective evidence of adhesive arachnoidi ...	1976	785039
[genetic transformation of streptococci].		1976	785057
a rationale for intraperitoneally administered antibiotic therapy.	a satisfactory clinical response occurred in 96.7 per cent of 210 patients with proved bacterial peritonitis following appropriate surgical intervention and an antimicrobial regimen of 1 per cent cephalothin administered intraperitoneally, with supplementary antibiotics as indicated. instillation of cephalothin achieves the therapeutic benefits of high intraperitoneal levels with an antibiotic of broad activity and minimum toxicity and permits flexibility in the choice of additional antimicrobia ...	1976	785645
[current hospitalismus. contribution to the causal factors of problem infection from the bacteriological view point].		1976	785871
[the influence of pivmecillinam on the human gut flora (author's transl)].	the effect of pivaloyloxymethyl ester of 6-beta-[(hexahydro-1h-azepin-1-yl)-methyleneamino]-penicillanic acid (pivmecillinam, fl 1039) on the gut flora of human subjects was investigated at different doses. following administration of 1.2 g or 2.4 g/die e. coli and other enterobacteriaceae species were markedly reduced. the response of enterococci and bacteroides species showed no uniformity. no overgrowth of the gut flora by pseudomonas, staphylococci or candida was observed. meanwhile it has b ...	1976	786300
formation of crystalline deposits by several genera of the family enterobacteriaceae.	several species of bacteria from the family enterobacteriaceae formed crystalline materials containing calcium when grown in a defined culture medium. enterobacter aerogenes, proteus vulgaris, citrobacter freundii, and c. intermedius produced calcium pyrophosphate crystals. edwardsiella tarda and escherichia coli formed calcite iii crystals, whereas proteus mirabilis, klebsiella pneumoniae, providencia stuartii, and serratia marcescens produced hydroxyapatite crystals. several of these bacteria ...	1976	786887
inhibitory effect of heparin on the limulus test for endotoxin.	heparin can inhibit the limulus test for endotoxin unless 0.05 m cacl(2) and 0.154 m nacl are added to the lysate.	1976	787001
rapid microbiologic assay of tobramycin.	different, rapid, plate diffusion assays of tobramycin in serum were compared with each other and with an overnight assay. these assays were done with klebsiella edwardsii var. atlantae, which was resistant to many antibiotics. one of the rapid assays of tobramycin tested used a 1:50-1:100 dilution of an overnight culture, and results were recorded after incubation for 5 hr at 35 c. two shorter assays were also tested with a 1:10 dilution of an overnight assay. the 5-hr assay was as reproducible ...	1976	787447
intrathecal amikacin administration. use in the treatment of gentamicin-resistant klebsiella pneumoniae meningitis.		1976	787564
adjuvant action of capsular polysaccharide of klebsiella pneumoniae on antibody response. v. further biological properties of the active substance.	the neutral fraction (neutral cps-k) of klebsiella pneumoniae capsular polysaccharide (cps-k) from type 1, kasuya strain, has already been reported as the active substance responsible for the strong adjuvant effect of cps-k. the present results demonstrate that neutral cps-k exhibits further common biological activities with lipopolysaccharide (lps) isolated from salmonella enteritidis. the intensity of the lethality in mice of neutral cps-k by the intraperitoneal route is very similar to that o ...	1976	787603
a naturally acquired infection of laboratory mice with klebsiella capsule type 6.	an epizootic causing high morbidity and high mortality in swiss white and nude mice over a period of 3 weeks was caused by klebsiella pneumoniae capsule type 6, which is considered to be nonpathogenic for mice. experimental inoculation of this organism into laboratory animals caused death within 36 hours, further substantiating its virulence.	1976	787669
[lytic properties of klebsiella ozaenae phages].		1976	787732
sensitivity patterns of urinary pathogens in the southland community and a study of sewage flora.	the overall sensitivity pattern of the three main urinary pathogens to the commonly used oral antibodies exceeded 80 percent. the incidence of multiple resistant escherichia coli was 8 percent. the resistant patterns of the sewage e. coli resembled that of the resistant urinary e. coli. however only one-third of the resistant urinary e. coli showed multiple resistance whereas two-thirds of the resistant e. coli in the sewage showed this feature. the resistance transfer was low in organisms isola ...	1976	787846
the bactericidal action of benzoic acid and sodium acetate on the gram-negative flora of dialysis fluid.	benzoic acid in concentrations from 0.18 per cent and upwards exerted a bactericidal action on the gram-negative flora of dialysis fluid at ph 4.75 in vitro. at a concentration of 0.32 per cent, a reduction of 10(5) was obtained with 24 hours. sulphonamide resistance did not affect the bactericidal action. the killing effect was hardly more than directly proportional to the concentration of benzoic acid. increasing inoculum decreased the bactericidal effect. therefore, benbzoic acid should not b ...	1976	788461
transferable and non-transferable drug resistance in enteric bacteria from hospital and from general practice.	drug resistance to 8 different antibiotics in enterobacteriaceae isolated from different hospitals and two groups of general practitioners was studied. escherichia coli dominated among the 632 strains investigated. drug resistance was found in 62% of the 512 hospital strains and in 38% of the 120 strains from general practice. multiple resistance was common especially in strains from hospital. r factors was found in 23% of the 317 drug-resistant strains from hospital and in 11% of the 46 drug-re ...	1976	775619
factors affecting the frequency infection in renal transplant recipients.	a retrospective review of 149 patients receiving 162 renal transplants showed that 83% of these patients developed one or more infections during a follow-up period averaging one year. in 32 (73%) of 44 deaths, infection was an important contributing cause. in only four (9%) of the deaths were the patients free of infection at the time of death. the klebsiella-enterobacter group was the most common agent causing pneumonitis and sepsis. cryptococcus neoformans caused seven of 11 cases of meningiti ...	1976	776110
[localization of antigens in common (cross-reacting) with neisseria perflava and klebsiella pneumoniae in the tissues of the human bronchopulmonary tract].	an attempt was made of detecting antigenic similarity beyween the fraction of microsomes of the tissues of human broncho-pulmonary apparatus and the cells of microbes of human respiratory tract. cross reactions of antimocrosomal sera were studied in the complement fixation test with neisseria perflava and klebsiella pneumoniae. fixation of antibacterial antibodies and antibodies against the microsomal fraction of the lung tissues was studied on the sections of the lung tissues ahd human bronchi. ...	1976	776254
donovanosis in papua new guinea.	clinical and epidemiological observations on 87 cases of donovanosis seen at port moresby general hospital are presented, with detailed reports of three cases in which the disease was more severe. the circumstances of infection described were consistent with venereal transmission. chloramphenicol and gentamicin were effective in curing the disease, while streptomycin was found to be ineffective in a number of cases tested. complement-fixation tests with donovania antigen revealed the presence of ...	1976	776356
study on the efficiency of four procedures for enumerating coliforms in water.	four coliform estimation procedures were compared as to their ability to enumerate coliform bacteria (oxidase-negative lactose fermenters) from sewage, effluent, and various fresh waters in the lower great lakes region of canada. two of the procedures used and which are cited in the american public health association (apha) standard methods were a most probable number (mpn) technique using lauryltryptose broth (lst) and brilliant green bile 2% broth (bgb), and a membrane filtration (mf) techniqu ...	1976	776372
activation of the complement system during infections due to gram-negative bacteria.		1976	776471
relationship between antibiotic consumption and frequency of antibiotic resistance of four pathogens--a seven-year survey.		1976	776921
a new serotyping method for klebsiella species: development of the technique.	a new serotyping method for klebsiella species using indirect immunofluorescence is described. nonspecific fluorescence has been minimized by carrying out the capsular antigen-antibody reaction at ph 9.0. commercial antisera have been tested with the 72 antigenic types of klebsiella, and appropriate dilutions of each pool and specific antisera have been proposed for use in routine typing. dilutions were chosen to allow strong fluorescence with each type and its specific antiserum and minimal flu ...	1976	777042
a new serotyping method for klebsiella species: evaluation of the technique.	a new indirect fluorescent typing method for klebsiella species is compared with an established method, capsular swelling. the fluorescent antibody (fa) technique was tested with standards and unknowns, and the results were checked by capsular swelling. several unknowns were sent away for confirmation of typing, by capsular swelling. the fa method was also tried by a technician in the routine department for blind identification of standards. fluorescence typing gives close correlation with the e ...	1976	777043
induction of resistance in staphylococcus aureus and klebsiella pneumoniae by exposure to cephalothin and cefoxitin.	six strains each of staphylococcus aureus and klebsiella pneumoniae were exposed to subinhibitory, but serially incremental, concentrations of cephalothin and cefoxitin in a totally defined liquid culture medium. after exposure to cephalothin, the level of resistance to both drugs increased; the gain was greater against cephalothin and among the strains of k. pneumoniae. after exposure to cefoxitin, resistance to both drugs developed; this resistance was greater against cefoxitin and among the s ...	1976	778303
influence of atmospheric oxygen concentration on acetylene reduction and efficiency of nitrogen fixation in intact klebsiella pneumoniae.	oxygen-limited (n2-fixing) chemostat cultures of klebsiella pneumoniae supplied with a n-free medium were established by introducing low atmospheric o2 concentrations into the gas supply of anaerobic glucose-limited n2-fixing chemostat cultures; the molar growth yield for glucose and the efficiency of n2 fixation (mug n fixed/mg glucose consumed) were increased (by up to 82%) from the anaerobic values. acetylene-reducing activity was inhibited reversibly by o2 in samples from o2-limited and anae ...	1976	778326
relationship of structure to function in bacterial endotoxins: serologically cross-reactive components and their effect on protection of mice against some gram-negative infections.	rabbit antisera were prepared against the heptoseless re mutants, salmonella minnesota r595 and s. typhimurium sli102, as well as against purified r595 glycolipid coated on autologous erythrocytes. the antisera cross-reacted with the endotoxic glycolipids extracted from re mutants of various bacterial strains, including s. minnesota r595, s. typhimurium sli102, escherichia coli d3im4, e. coli d2if2 and e. coli f515, as shown by passive haemagglutination and gel diffusion tests. the anti-re sera ...	1976	778329
the importance of prolonged incubation for the synthesis of dimethylnitrosamine by enterobacteria.	tests with 140 strains representing escherichia coli, klebsiella aerogenes, k. ozaenae, proteus mirabilis, p. vulgaris, p. rettgeri and p. morganii in a defined medium supplemented with 0-09m dimethylamine (dma) and 0-1m potassium nitrate showed that at least 89% of the 136 strains able to reduce nitrates produced up to 9mm dimethylnitrosamine (dmn) in 70h at 37 degrees c. four nitratase-negative strains produced dmn from dma in the presence of sodium nitrate. prolonged incubation was the most i ...	1976	778390
the influence of antibiotic treatment on resistance patterns of coliform bacilli in childhood urinary-tract infection.	the occurrence of coliform bacilli carrying resistance-transfer factors (r factors) in children was studied. the frequency of r+ coliform bacilli as causes of urinary-tract infection acquired outside hospital was found to be similar to that in adults from the same geographical area and in the same years. the frequency of r+ coliform bacilli in the faeces in our children was also similar to that in the adult population, and oral chemotherapy produced similar changes in the faecal flora.	1976	778392
[studies on the intravenous administration of sulbenicillin (author's transl)].	basic and clinical investigation on the intravenous administration of sulbenicillin in moderate dose (510g daily) was carried out to evaluate its clinical effect in systemic infections due to gram-negative bacilli. the following results were obtained. (1) in human subjects received 5 g intravenous drip infusion, the peak blood levels were found at the end of infusion. in 6 cases with normal renal function (ccr greater than or equal to 70ml/min.) the peak blood level was 181 mcg/ml on the average ...	1976	778428
[experimental studies of chronic rheumatoid arthritis--experimental models of arthritis].		1976	778442
therapy of infections in neutropenic patients: results with gentamicin in combination with cephalothin or chloramphenicol.	gentamicin in combination with cephalothin (gent-ceph) or with chloramphenicol (gent-chloro) was utilized in the treatment of 55 infections occurring in 49 cancer patients. responses were obtained in 78% of the infections treated with gent-ceph and in 64% of those treated with gent-chloro. pneumonia and septicemia were the most common infections in this study. among the cases of penumonia, 64% responded to gent-ceph and 67% to gent-chloro. among the cases of septicemia, 88% responded to gent-cep ...	1976	778574
contamination of the small intestine by enterotoxigenic coliform bacteria among the rural population of haiti.	coliform bacteria were isolated by either aerobic or anaerobic culture techniques from aspirates of the proximal small intestine of 4 of 5 haitians with tropical sprue, but not from any of 10 well nourished haitians who had milder gastrointestinal complaints and abnormalities. klebsiella (klebsiella pneumoniae and klebsiella ozaenae) was cultured from the jejunal aspirates of 2 sprue patients and escherichiae coli from the other 2. fifteen colonies of coliform bacteria cultured from each aspirat ...	1976	773737
a clinical study of primary infection on the lungs and pleura by friedlander's bacillus.		1976	773813
failure of endotoxin to increase nonspecific resistance to infection of lipopolysaccharide low-responder mice.	in vitro and in vivo responses to lipopolysaccharide (lps) and various other bacterial immunostimulants were compared in c3h/he low-responder mice. the principal findings were as follows. (i) their splenic lymphocytes were stimulated by various gram-negative mitogens such as an escherichia coli peptidoglycan, a detoxified derivative of lps, and even endotoxins extracted by trichloroacetic acid that are known to contain protein; spleen cells of these mice were also transformed by two other b-cell ...	1976	773822
subunit and chemical composition of citrate lyase from klebsiella pneumoniae.	citrate lyase from klebsiella pneumoniae (previously called klebsiella aerogenes) is shown by amino acid sequencing techniques to contain equimolar amounts of three nonidentical subunits. together with the molecular weight of the enzyme, this finding is interpreted to indicate that there are 6 mol of each of these subunits per mol of native enzyme. a partial amino acid sequence of each of the three subunits is presented. the pure enzyme with a specific activity of 73 units/mg of dry protein has ...	1976	773936
[revaluation of gentamicin in laboratory and clinical studies (author's transl)].	the mic of gentamicin and other antibiotics against clinical isolates was determined, and the following sensitivity distribution was obtained. a) against 54 strains of staphylococcus aureus, gm had the lowest mic, followed by tob, dkb, bb-k8 in order. the distribution range of 0.1-6.25 mug/ml was noted with gm. the peak of mic was 1.56 mug/ml. b) against 54 strains of e. coli, the lowest mic was obtained with gm, and 53 strains (98%) were in the range of 0.4-6.25 mug/ml. the peak of mic was 3.12 ...	1976	775143
bacterial mastitis in guinea pigs.	necropsy of 37 guinea pigs revealed mastitis to be the primary cause of death in 70% of these animals. epidemiologic study failed to establish correlation between age, parity, or litter size and the onset of mastitis. the disease did not appear to be contagious. although mastitis frequently developed during lactation, survivability of offspring was unaffected. the agent most consistently isolated from affected mammary glands was escherichia coli (17 of 37). other isolates in decreasing order of ...	1976	775191