Publications

molecular diversity of human parvovirus b19 during two outbreaks of erythema infectiosum in brazil.	this study was conducted to provide information on the genetic diversity of human parvovirus b19 (b19v) circulating in the municipality of niterói, rio de janeiro, southeast brazil during 1996-2006, a period with two distinct outbreaks of b19v infection: 1999-2000 and 2004-2005. a total of 27 sera from patients with erythema infectiosum and five sera from hiv-infected patients that tested positive for b19v dna during the study period were analyzed. to genotype b19v strains, a semi-nested pcr for ...	2017	27914222
does parvovirus infection have a role in systemic lupus erythematosus?	we sought to evaluate a possible link between parvovirus b19 infection and the clinical and laboratory expression of systemic lupus erythematosus (sle). sle patients were examined to evaluate their clinical status and disease activity. a complete systemic lupus erythematosus disease activity index (sledai) score was obtained for each patient. in addition, we determined the level of systemic involvement throughout the course of the disease. blood levels of igm and igg antibodies to parvovirus b19 ...	2017	28116652
successful outcome of three patients with sickle-cell disease and fat embolism syndrome treated with intensive exchange transfusion.	fat embolism syndrome (fes) is a rare complication of sickle-cell disease (scd) associated with extremely high mortality rates. it affects predominantly non-ss patients and those with previously mild disease. rapid institution of exchange transfusion with an aim to reduce hbs to very low levels as soon as fes is suspected can be life-saving.	2017	28096988
simultaneous occurrence of autoimmune hemolytic anemia and pure red cell aplasia.	simultaneous onset of autoimmune hemolytic anemia (aiha) and pure red cell aplasia (prca) is rare and any possible association between these two disorders remains obscure. a 46-year-old previously healthy woman was diagnosed as having aiha based on severe anemia, positive direct and indirect coomb's tests, decreased serum haptoglobin, elevated serum ldh, and indirect bilirubin-dominant hyperbilirubinemia. oral steroid administration (1 mg/kg) and subsequent half-pulse steroid therapy ameliorated ...	2017	28090019
[collapsing variant of focal segmental glomerulosclerosis by parvovirus b19: case report].	to describe the clinical and laboratory profile of focal segmental glomerulosclerosis (fsgs) of the collapsing subtype in association with infection by parvovirus b19 (pvb19).	2017	25923759
pure red cell aplasia due to parvovirus b19: erythropoietin-resistant anemia in a pediatric kidney recipient.		2017	28387173
hydroxyurea inhibits parvovirus b19 replication in erythroid progenitor cells.	parvovirus b19 (b19v) infection is restricted to erythroid progenitor cells (epcs) of the human bone marrow, leading to transient arrest of erythropoiesis and severe complications mainly in subjects with underlying hematological disorders or with immune system deficits. currently, there are no specific antiviral drugs for b19v treatment, but identification of compounds inhibiting b19v replication can be pursued by a drug repositioning strategy. in this frame, the present study investigates the a ...	2017	28377277
extinct type of human parvovirus b19 persists in tonsillar b cells.	parvovirus b19 (b19v) dna persists lifelong in human tissues, but the cell type harbouring it remains unclear. we here explore b19v dna distribution in b, t and monocyte cell lineages of recently excised tonsillar tissues from 77 individuals with an age range of 2-69 years. we show that b19v dna is most frequent and abundant among b cells, and within them we find a b19v genotype that vanished from circulation >40 years ago. since re-infection or re-activation are unlikely with this virus type, t ...	2017	28374737
persistent symptomatic parvovirus b19 infection with severe thrombocytopenia transmitted by red blood cell transfusion containing low parvovirus b19 dna levels.	transfusion-mediated human parvovirus b19 (pvb19) infection is rare but often causes severe hematologic disorders. in japan, routine blood donor screening for pvb19 antigen (detection sensitivity, 10(6.4) iu/ml) using a chemiluminescent enzyme immunoassay (cleia) was introduced in 2008. however, there is no consensus on the minimal infectious dose of pvb19 permissible for red blood cells (rbcs).	2017	28369973
corrigendum: the plasma virome of febrile adult kenyans shows frequent parvovirus b19 infections and a novel arbovirus (kadipiro virus).		2017	28366188
increased numbers of cd4(+)cd25(+) and cd8(+)cd25(+) t-cells in peripheral blood of patients with rheumatoid arthritis with parvovirus b19 infection.	to investigate t-cell subpopulations in peripheral blood of human parvovirus b19 dna-positive (b19(+)) and -negative (b19(-)) patients with rheumatoid arthritis (ra) and healthy persons.	2017	28358698
acquired factor viii deficiency: two case reports and a review of literature.	acquired factor viii (fviii) deficiency, or acquired hemophilia a (aha), is a rare autoimmune disorder involving antibody-mediated depletion of coagulation fviii, leading to severe, life-threatening bleeding. the condition is often associated with other autoimmune disorders, and its treatment involves replacement of fviii and various modes of immunosuppression. recently, a few noteworthy therapeutic advances have been made. we present two cases of severe aha in chinese women. one of these women ...	2017	28352491
parvovirus b19 infection modulates the levels of cytokines in the plasma of rheumatoid arthritis patients.	parvovirus b19 (b19v) infection is associated with various autoimmune diseases. we investigated the levels of pro-inflammatory (ifnᵧ, tnfα, il-2, il-12) and anti-inflammatory (il-4, il-10) cytokines in the plasma of b19v dna positive (b19(+)) and negative (b19(-)) rheumatoid arthritis (ra) patients in comparison with the control group (healthy persons).	2017	28288327
parvovirus b19 ns1 protein induces cell cycle arrest at g2-phase by activating the atr-cdc25c-cdk1 pathway.	human parvovirus b19 (b19v) infection of primary human erythroid progenitor cells (epcs) arrests infected cells at both late s-phase and g2-phase, which contain 4n dna. b19v infection induces a dna damage response (ddr) that facilitates viral dna replication but is dispensable for cell cycle arrest at g2-phase; however, a putative c-terminal transactivation domain (tad2) within ns1 is responsible for g2-phase arrest. to fully understand the mechanism underlying b19v ns1-induced g2-phase arrest, ...	2017	28264028
the formation and modification of chromatin-like structure of human parvovirus b19 regulate viral genome replication and rna processing.	b19 virus (b19v) is a single stranded virus in the genus of erythroparvovirus in the family of parvoviridae. one of the limiting steps of b19v infection is the replication of viral genome which affected the alternative processing of its rna. minute virus of mice (mvm) and adeno-associated virus (aav) has been reported to form chromatin-like structure within hours after infection of cells. however, the role of chromatin-like structure is unclear. in the present study, we found that b19v formed ch ...	2017	28263841
massive erythrophagocytosis by peripheral monocytes and neutrophils in parvovirus-b19 autoimmune hemolytic anemia.		2017	28224193
detection of human parvovirus b19 dna in 22% of 1,815 cutaneous biopsies of a wide variety of dermatologic conditions suggests viral persistence after primary infection and casts doubts on its pathogenic significance.	human parvovirus b19 (b19v) has been associated with a number of dermatologic and systemic conditions, including myocarditis and autoimmune syndromes.	2017	28196283
severe anemia due to parvovirus b19 in a silver haired boy.	griscelli syndrome (gs) is a rare autosomal recessive immunodeficiency disorder in which the affected children present with characteristic silvery-white hairs. the hair microscopy of these children is characteristic and is helpful in differentiating gs from chediak-higashi syndrome which also presents with immunodeficiency and silver hairs. we report a 17-month-old boy with gs type 2 who presented with severe anemia. bone marrow examination of the child suggested parvovirus b19 as the cause of s ...	2017	26960654
chimeric external control to quantify cell free dna in plasma samples by real time pcr.	dna isolation procedure can significantly influence the quantification of dna by real time pcr specially when cell free dna (cfdna) is the subject. to assess the extraction efficiency, linearity of the extraction yield, presence of co-purified inhibitors and to avoid problems with fragment size relevant to cfdna, development of appropriate external dna control (edc) is challenging. using non-human chimeric nucleotide sequences, an edc was developed for standardization of qpcr for monitoring stab ...	2017	27141267
seroprevalence of erythrovirus b19 in saudi pregnant women.	erythrovirus b19 infection is associated with clinical symptoms that range from mild to severe. the common clinical presentation of b19 virus (b19v) infection is erythema infectiosum, arthropathy, aplastic crisis, and fetal infection. infection in seronegative pregnant women can lead to fetal hydrops.	2017	27186157
hypocomplementemia is a diagnostic clue for parvovirus b19 infection in adults.		2017	27238731
carbimazole-induced agranulocytosis.	a 47 year old lady with hyperthyroidism for past 1½ years was initially on carbimazole 20 mg orally then changed to 30 mg (during hysterectomy) but was taking 10 mg for last 1 year. she had intermittent fever with severe b/l bifrontal headache since 3 weeks. routine investigations showed anaemia, neutropenia, leucopenia and crp elevation. peripheral smear showed normocytic normochromic anaemia with rouleaux formation, leucopenia with 2% atypical cells and mild thrombocytosis. widal test, ra fact ...	2017	26813922
environmental issues in thyroid diseases.	environmental factors are determinant for the appearance of autoimmune thyroid diseases (aitd) in susceptible subjects. increased iodine intake, selenium, and vitamin d deficiency, exposure to radiation, from nuclear fallout or due to medical radiation, are environmental factors increasing aitd. cigarette smoking is associated with graves' disease and graves' ophthalmopathy, while it decreases the risk of hypothyroidism and thyroid autoimmunity. viral infections are important environmental facto ...	2017	28373861
the blood dna virome in 8,000 humans.	the characterization of the blood virome is important for the safety of blood-derived transfusion products, and for the identification of emerging pathogens. we explored non-human sequence data from whole-genome sequencing of blood from 8,240 individuals, none of whom were ascertained for any infectious disease. viral sequences were extracted from the pool of sequence reads that did not map to the human reference genome. analyses sifted through close to 1 petabyte of sequence data and performed ...	2017	28328962
[myocarditis].	acute myocarditis must be considered in patients with recent onset of cardiac failure or arrhythmia. fulminant myocarditis is a distinct entity characterized by sudden onset of severe congestive heart failure or cardiogenic shock, usually following a flu-like illness, parvovirus b19, human herpesvirus 6, coxsackie virus and adenovirus being the most frequently viruses responsible for the disease. in this setting, early recognition of patients rapidly progressing to refractory cardiac failure and ...	2017	28161113
anaemia in hospitalised preschool children from a rural area in mozambique: a case control study in search for aetiological agents.	young children bear the world's highest prevalence of anaemia, the majority of which is of multifactorial aetiology, which in turn hampers its successful prevention. even moderate degrees of anaemia are associated with increased mortality and morbidity. despite this evidence, there is a lack of effective preventive programs and absence of consensus in the safety of iron supplementation in malaria areas, which reflects the poor understanding of the contribution of different aetiologies to anaemia ...	2017	28241813
virus reactivations after autologous hematopoietic stem cell transplantation detected by multiplex pcr assay.	several studies have indicated that viral reactivations following allogeneic hematopoietic stem cell transplantation (allo-hsct) are frequent, but viral reactivations after autologous hsct (auto-hsct) have not been investigated in detail. we performed multiplex polymerase chain reaction (pcr) assay to examine multiple viral reactivations simultaneously in 24 patients undergoing auto-hsct between september 2010 and december 2012. weekly whole blood samples were collected from pre- to 42 days post ...	2017	27364410
characterization of antibodies in human immunoglobulin products from different regions worldwide.	the antibody levels against a broad spectrum of pathogens were assessed in commercial intravenous immunoglobulin (ivig) manufactured from pooled plasma obtained from different global regions.	2021	33524620