Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the leeuwenhoek lecture, 1983. a prototype vaccine to prevent epstein-barr virus-associated tumours. | eb virus is a herpesvirus that infects all human communities. the infection is life-long and usually asymptomatic. excessive reaction to primary infection leads to infectious mononucleosis while immunological failures give fatal lymphoproliferative diseases. the virus is associated with endemic burkitt's lymphoma and undifferentiated nasopharyngeal carcinoma. in world cancer terms burkitt's lymphoma is insignificant, but nasopharyngeal carcinoma has a high incidence in certain important populati ... | 1984 | 6144103 |
| screening for nasopharyngeal carcinoma. | 1984 | 6145937 | |
| the dna sequence of epstein-barr virus. | 1984 | 6146870 | |
| cryptogenic fibrosing alveolitis and epstein-barr virus: an association? | 13 patients with cryptogenic fibrosing alveolitis (cfa) and 12 with interstitial lung disease (ild) of known cause were studied for their humoral response to herpes simplex virus (hsv), cytomegalovirus (cmv), and epstein-barr virus (ebv). serum antibodies to hsv and cmv were within the normal range in all patients. 10 patients with cfa had raised serum antibodies to ebv, and iga against viral-capsid antigen (vca) was detectable in all 13. in the other 12 patients ebv serological profiles were no ... | 1984 | 6148520 |
| wiskott-aldrich syndrome and epstein-barr virus-induced lymphoproliferation. | 1984 | 6148654 | |
| [demonstration of igg- and iga-antibodies against epstein-barr virus-associated antigens with a microtiter enzyme immunoassay system. the determination of serum antibodies against the viral capsid antigen and the early antigen complex in the sera of tumor and infectious mononucleosis patients]. | an enzyme-linked immunosorbent assay (elisa) using micro-titre plates has been established for determination of igg and iga antibodies to the epstein-barr virus (ebv)-associated early antigen complex (ea) and the viral capsid antigen (vca). the results obtained correlated well with the antibody titre determinations by the standard immunofluorescence technique. the elisa was found to be about 15 to 30 times more sensitive than the immunofluorescence assay. because of its high sensitivity, specifi ... | 1984 | 6094138 |
| ultrastructural studies on human lymphoblastoid cells treated with n-butyrate and 12-o-tetradecanoylphorbol-13-acetate. | the effects of n-butyrate and/or 12-o-tetradecanoylphorbol-13-acetate (tpa) on raji and p3hr-1 cells were examined using electron microscopy. n-butyrate treatment caused a decrease in the number and elongation of surface microvilli. in addition, well-developed rough endoplasmic reticulum and a marked decrease in condensed chromatin clumps were observed in the nucleus. the filamentous structures and nuclear membrane-like structures, which were not reported previously, appeared occasionally in the ... | 1984 | 6094198 |
| identification of several functional subgroups of hla-b27 by restriction of the activity of antiviral t killer lymphocytes. | anti-epstein-barr virus and antiinfluenza a cytotoxic t lymphocytes (ctl) have been used to study the restriction of human antiviral responses by hla-b27 antigens. three functional subgroups of hla-b27 have been clearly distinguished by this "restriction-typing assay". no cross-reaction could be detected between the three subgroups either at the ctl level or at the level of antigen-presenting cells. the cells of subgroup 1 are always positive [m2(+)] when tested in immunofluorescence with a mono ... | 1984 | 6094341 |
| early primary infection and high epstein-barr virus antibody titers in greenland eskimos at high risk for nasopharyngeal carcinoma. | in a comparative study of populations at high and low risk of nasopharyngeal carcinoma (npc), sera from 442 eskimo and 770 danish children and adolescents were tested for the presence of antibodies against epstein-barr virus (ebv). eskimo children in greenland were seropositive at an early age and showed significantly higher titers of igg antibody to the viral capsid antigen (vca) (p less than 0.0001) and soluble (s) antigen (p less than 0.005) than danes matched for age and sex, but had similar ... | 1984 | 6094363 |
| a human nucleolar antigen (pp90) associated with cell growth and its induction by epstein-barr virus and human cytomegalovirus. | immunofluorescent staining of hela cells with rabbit antiserum raised against isolated hela cell nucleoli showed bright nucleolar fluorescence. immunoprecipitation of nuclear extracts obtained from hela cells labelled with 35s-methionine or 32p-orthophosphate followed by gel electrophoresis of the precipitate revealed a major band of 90 kd. this antigen, called pp90, was judged to be responsible for the nucleolar fluorescence. serine residues were predominantly phosphorylated in pp90. similar nu ... | 1984 | 6094364 |
| the production of human monoclonal autoantibodies from patients with rheumatoid arthritis by the ebv-hybridoma technique. | rheumatoid lymphocytes tend to transform 'spontaneously' in vitro because of prior infection with epstein barr virus (ebv). they are particularly difficult to use in experiments involving cell hybridisation, because in the conventional half-hat system unfused transformed cells may be confused with hybrids. we describe how the hat-sensitive, ouabain-resistant human b lymphoblastoid cell line kr4, originally developed to 'rescue' ebv induced b cell clones, can be fused successfully with peripheral ... | 1984 | 6094669 |
| infectious mononucleosis-like response in common marmosets infected with epstein-barr virus. | callithrix jacchus marmosets infected with epstein-barr virus (ebv) with or without concurrent treatment with cyclosporin a (cysa) remained healthy. five marmosets given virus alone developed lymphocytosis and heterophile antibody. antibody to ebv capsid antigens (vca) appeared and remained at titers of 1:40-1:80 from 15 weeks onward. two animals produced antibody to the r component of early antigens (ea) from six weeks onward. five cysa-treated ebv-infected marmosets showed no increase in total ... | 1984 | 6094675 |
| pure red blood cell aplasia associated with chronic epstein-barr virus infection: evidence for t cell-mediated suppression of erythroid colony forming units. | a prominent t cell suppressor response is known to develop and inhibit the polyclonal b cell activation induced by epstein-barr virus infection. exuberant t suppressor cell activity suppressing erythropoiesis has been demonstrated in certain cases of pure red blood cell aplasia. we studied a 19-year-old man who developed pure red cell aplasia after epstein-barr virus infection. over a 70-week period, lymphocyte function and serologic evidence for chronic epstein-barr virus infection was demonstr ... | 1984 | 6094693 |
| interaction between epstein-barr virus-determined nuclear antigen (ebna) and the viral dna. | epstein-barr virus (ebv) nuclear antigen (ebna) was purified from the burkitt lymphoma line raji and its ebv dna-binding properties were characterized. ebna binding protected fragments of about 30 bp of b95-8 cell-derived ebv dna from an excess of dnase i. human anti-ebna antibodies prevented dna binding. purified extracts from ebna-negative cells did not protect ebv dna against dnase i digestion. mapping of the ebv dna fragments protected from endonuclease (ecori, hindiii, sali) digestion revea ... | 1984 | 6094716 |
| virologic studies in a case of transfusion-associated aids. | the authors present the case of a 60-year old woman who developed acquired immunedeficiency syndrome (aids) 43 months after a blood transfusion. the blood donor was identified as a sexually active homosexual man who frequently used intravenous drugs. although he was asymptomatic, physical examination revealed generalized lymphadenopathy of cervical, axillary, and inguinal areas. 47 months after his blood donation, the donor underwent lymph node biopsy which revealed follicular hyperplasia. h ... | 1984 | 6092957 |
| perinatal herpesvirus infections. | 1984 | 6093031 | |
| viral infections in immunocompromised children. | 1984 | 6093032 | |
| [comparative evaluation of technics for detection of ebv-dna in exfoliated nasopharyngeal cells]. | 1984 | 6100480 | |
| generating human monoclonal antibodies. | present methods and systems for the generation of human monoclonal antibodies are briefly reviewed. the specificities of the available reagents are outlined. it would appear that the generation using hybridoma methods of human monoclonal antibodies to human tumor cell surface antigens is a rare event and that methods of in vitro immunostimulation may have to be used if such antibodies are to be reliably produced. | 1984 | 6100557 |
| human retrovirus-induced il-2 receptors and their possible role in transduction of continuous cell growth signal. | the first half of this report describes direct evidence for the interleukin 2 receptor (il-2r)-inducing ability of human t-cell leukemia/lymphoma virus (htlv). when an epstein-barr virus (ebv)-transformed human b-cell line, lcl-kan was infected with htlv or transfected with plasmid dna containing defective htlv genome (long terminal repeat (ltr)-gag-px-ltr), lcl-kan cells were altered to express il-2r which was indistinguishable from that of normal activated t cells. the second half of this pape ... | 1984 | 6100645 |
| lymphadenopathy associated virus and its etiological role in aids. | lymphadenopathy associated virus (lav) is a novel human retrovirus first reported in 1983. it was isolated from the lymph node lymphocytes of a french homosexual patient with generalized hyperplasic lymphadenopathy. subsequently lav was isolated from patients with frank acquired immune deficiency syndrome (aids) coming from all the different high-risk groups, while anti-lav antibodies were detected equally in individuals from all "at-risk" groups. such a profile is consistent with the virus bein ... | 1984 | 6100650 |
| [burkitt's lymphoma]. | 1984 | 6100729 | |
| human t cell leukemia virus type i and epstein-barr virus antibodies in homosexuals and hemophilia patients from venezuela. | the presence of serum antibodies to human t-cell leukemia/lymphoma virus-i (htlv-i) and to epstein-barr virus (ebv) was studied in homosexuals and hemophilia patients from the state of zulia (venezuela), a highly endemic area for htlv-i infection. no serum antibodies to htlv-i were detected despite the presence of alterations in peripheral blood lymphocyte subsets. anti-ebv antibodies in homosexuals were indicative of persistent infection with this virus. our results suggest that htlv-i does not ... | 1984 | 6100781 |
| in vivo epstein barr virus-induced augmentation of natural killer cell activity in the chédiak-higashi syndrome. | 1984 | 6197464 | |
| interferon and natural killing of human lymphoma cell lines after induction of the epstein barr viral cycle by superinfection. | interferon (ifn) production during natural killer (nk) cell assays with raji, an ebv-carrying human lymphoma-derived cell line, was studied to determine whether ifn generated by effectors in vitro acted in target cell lysis. in 4-hr tests, raji is insensitive to nk but becomes susceptible after superinfection with the p3hr-1 strain of ebv. ifn was not detectable by bioassay in supernatants from 4-hr assays, and the addition of antibody to ifn did not prevent the lysis of the superinfected raji c ... | 1984 | 6197476 |
| rapid expansion of human cytotoxic t cell clones: growth promotion by a heat-labile serum component and by various types of feeder cells. | culture conditions are described that result in rapid expansion of cloned cytotoxic t cells of human origin. a combination of allogeneic lymphocytes and epstein-barr virus (ebv) transformed b cells (b-lcl) as irradiated feeder cells resulted in a 10-fold higher cell yield than obtained by use of either feeder cell alone. the large cell numbers obtained in a relatively short period of time facilitate in vitro and in vivo experimentation. further enhancement of cell proliferation was obtained by t ... | 1984 | 6197484 |
| target level blocking of t-cell cytotoxicity for human malignant melanoma by monoclonal antibodies. | cytotoxic t lymphocytes (ctl) for autologous malignant melanoma in culture of a patient av were induced by restimulation of pbl (peripheral blood leukocytes) with av melanoma cells in vitro and subcultured in interleukin 2 (il-2) conditioned media. monoclonal antibodies detecting six antigenic systems on melanoma cell surfaces were tested for blocking activity on the effector function of subcultured cytolytic t lymphocytes for autologous melanoma cells. the monoclonal antibodies r24 (gamma 3), s ... | 1984 | 6198103 |
| human cytotoxic t lymphocytes (ctl) against epstein-barr virus (ebv) infected cells: ebv specificity and involvement of major histocompatibility complex determinants in the lysis exerted by anti-ebv ctl toward hla-compatible and allogeneic target cells. | human peripheral blood lymphocytes (pbl), from anti-epstein-barr virus (ebv)-seropositive donors, were stimulated by ebv and were shown to be cytotoxic toward autologous, hla-compatible, and fully allogeneic ebv-transformed target cells. the lysis was not due to natural killer (nk) cells since the target cells used were resistant to lysis by fresh pbl and by virus-stimulated pbl-depleted of aet-srbc-rosetting t cells (the latter being still fully cytotoxic on k562 nk-susceptible target cells). c ... | 1984 | 6198104 |
| heterogeneity of the human phosphocholine-specific b cell repertoire. | we have utilized a highly efficient method of culturing small numbers of epstein barr virus (ebv)-infected cells to analyze the heterogeneity of human antibodies specific for phosphocholine (pc). lymphocytes from peripheral blood or tonsils of individuals who had no evidence of recent pneumococcal infection were infected with ebv and cultured at limiting dilution. after correction for the cloning efficiency, between 1/1500 and 1/10,000 b cells produced specific anti-pc antibodies by our criteria ... | 1984 | 6198388 |
| virologic, immunologic, and clinical observations on a patient during the incubation, acute, and convalescent phases of infectious mononucleosis. | one patient with infectious mononucleosis (im) was studied from the probable time of epstein-barr virus (ebv) infection (38 days before the onset of clinically overt disease), during the incubation and acute phases, until 6 months after clinical remission. analysis of spontaneous outgrowth of ebv-carrying lymphoblastoid cells, by limiting dilution on feeder layer cultures, showed that virus containing b lymphocytes are already present early during the incubation period. also low interferon serum ... | 1984 | 6199144 |
| analysis of proteins, helper dependence, and seroepidemiology of a new human parvovirus. | a new type of defective parvovirus, tentatively designated as adeno-associated virus type 5 (aav-5), is characterized as far as its proteins, its helper dependence, and its seroepidemiology are concerned. the protein analysis of aav-5 in polyacrylamide gels demonstrated the presence of three structural polypeptides, corresponding to vp 1, vp 2, and vp 3 of other aav types. the preparation of monoclonal antibodies against aav-5 permitted the analysis of viral structural antigen expression by usin ... | 1984 | 6200995 |
| recognition of autologous lymphoblastoid cells by cloned human t cells: inhibition of cytotoxicity by anti-hla antibody but not by antibody to epstein-barr virus induced cell surface antigen. | cytotoxic t cells (tc) can be primed in vitro to specifically kill autologous epstein-barr virus (ebv) transformed lymphoblastoid cell line cells (lcl). the antigen recognized by tc on the lcl cells is designated lydma (lymphocyte determined membrane antigen). recognition of lydma may also require simultaneous recognition of hla determinants. despite its immunogenic potency, the genetic origin and molecular structure of lydma remains undetermined. most lcl cells do not produce ebv virus, but all ... | 1984 | 6201468 |
| rescue of human monoclonal antibody production from an ebv-transformed b cell line by fusion to a human-mouse hybridoma. | a human-mouse cell line that is hypoxanthine-aminopterin-thymidine sensitive and ouabain resistant was derived from a fusion between human b lymphocytes and a mouse myeloma line. this new mutant, when fused to a relatively unstable ebv-transformed b cell secreting a human monoclonal anti-a (red blood cell antigen) antibody, resulted in stable hybridomas capable of long term production of the specific human monoclonal antibody. furthermore, some of the hybrid clones secreted antibody in far great ... | 1984 | 6201562 |
| a hodgkin cell-specific antigen is expressed on a subset of auto- and alloactivated t (helper) lymphoblasts. | a hodgkin cell-specific antigen detected by the monoclonal antibody ki-1 was found on t helper lymphocytes after activation by autologous and allogeneic stimulator cells. about 50% of lymphoblasts generated by auto- and alloactivation reacted with the antibody. in contrast, only less than 6% of lymphoblasts stimulated with con-a, phytohemagglutinin (pha), or protein a, and none of lymphoblasts activated by oxidative mitogenesis, expressed this antigen. among several permanent cell lines tested, ... | 1984 | 6202342 |
| production of antibody to human osteosarcoma associated antigens by continuous human lymphoblastoid cell lines. | human lymphoblastoid cell lines that produce specific antibody against human osteosarcoma associated plasma membrane antigens have been established by preselecting osaa binding human b lymphocytes from an osteosarcoma patient followed by epstein-barr virus (ebv) transformation. four cell lines ( kla -1, kla -2, kla -3, kla -4) were obtained and cloned. the antibodies were lambda light containing igm. the specificities of the antibodies were confirmed by immunofluorescence assays using tumor cell ... | 1984 | 6202625 |
| evidence for polymorphism of mb3 antigens among three hla-d clusters associated with hla-dr4. | in a previous study, we showed that the three hitherto serologically indistinguishable hla-d specificities associated with hla-dr4, hla- dyt , hla- dkt2 , and hla-dw4 can be distinguished on the basis of their reactivity with two distinct ia-like-specific monoclonal antibodies, hu-18 and hu-23. in this study, we attempted to identify and characterize ia-like molecules recognized by hu-18 and hu-23 on a molecular level because ia subsets (hla-dr, mb, mt, or sb) identified by them remained unknown ... | 1984 | 6202628 |
| induction of plasmacytoid differentiation by phorbol ester in b-cell lymphoma cell lines bearing 8;14 translocations. | at nanomolar concentrations, phorbol 12-myristate 13-acetate induced differentiation in a human epstein-barr virus-negative b-cell line, jd 38, derived from an undifferentiated lymphoma and containing an 8;14 translocation. the changes induced by phorbol 12-myristate 13-acetate were consistent with differentiation towards plasma cells and included (i) a marked increase (30-fold) in igm secretion; (ii) a decrease in the nuclear/cytoplasmic ratio associated with the development of a single promine ... | 1984 | 6203124 |
| diagnosis and management of viral infections. | with the introduction of new diagnostic techniques and the expanding choices of antiviral agents, clinical virology is rapidly becoming an important field in infectious diseases. while effective therapy for several respiratory viruses, cytomegalovirus, hepatitis b virus, and epstein-barr virus is not currently available, herpes simplex, varicella zoster, and influenza a associated diseases can be adequately treated in many cases at the present time. new agents such as interferon, other nucleosid ... | 1984 | 6203680 |
| transcription and dna sequence of the bamhi l fragment of b95-8 epstein-barr virus. | the sequence of the bamhi l fragment of b95-8 epstein-barr virus (ebv) dna has been determined. the transcription starts of five promoters have been mapped to this fragment, using s1 mapping and either in vitro transcription or the primer extension technique. dramatically increased levels of cytoplasmic poly(a) + rnas, transcribed from these promoters, occur after treatment of b95-8 cells with 12-o-tetradecanoylphorbol-13-acetate (tpa). for three of these promoters addition of phosphonoacetic ac ... | 1984 | 6203743 |
| idiotypic determinants on human thyroglobulin autoantibodies derived from the serum of hashimoto patients and eb virus transformed cell lines. | rabbit anti-idiotypic reagents were prepared against three different human serum autoantibodies to thyroglobulin (tg). two of the rabbit antisera recognized private idiotypes (idi) whilst a third antiserum recognized both an idi and a cross-reactive idiotype (idx) which was expressed in 50% of hashimoto patients tested. a fourth anti-idiotype was produced against an igm anti-tg secreted by a patient's epstein-barr (eb) virus transformed lymphocytes. this antiserum only reacted with the immunizin ... | 1984 | 6204801 |
| hla-dw/ld directed cytotoxic t cell clones. | t lymphocyte clones were derived by micromanipulation from an mlc between a stimulator and responder matched for class i but mismatched for class ii hla antigens; among the possible stimulating antigens were dr4 and dw4. two of the clones, when tested for cytotoxicity on a panel of dr4 positive and negative targets, appeared to recognize a determinant closely associated with dw4, but did not lyse, with one exception, targets expressing other dr4 associated dw specificities or dr4 negative target ... | 1984 | 6204957 |
| human t cell clones exerting multiple cytolytic activities show heterogeneity in susceptibility to inhibition by monoclonal antibodies. | human cytotoxic t cell clones (ctl) were obtained by limiting dilution after in vitro priming against an allogeneic epstein barr virus (ebv)-transformed b cell line (b-lcl) bsm. three okt3+, okt8+ e rosette-forming (rfc) but ea gamma-rfc- clones with cytotoxic activity against the stimulator cell and one "non-cytolytic" clone were expanded for over 50 generations and further characterized. clone g9 showed allospecific lysis of cw3+ lymphocytes and b cell lines. three cytolytic clones (g9, d11, a ... | 1984 | 6205072 |
| antibodies against a synthetic peptide identify the epstein-barr virus-determined nuclear antigen. | five peptides corresponding to amino acid sequences predicted from all three reading frames of the nucleotide sequence of the third internal repeat array (ir3) of the epstein-barr virus (ebv) genome were synthesized chemically. all five peptides elicited antipeptide antibodies in rabbits. the antiserum raised against a 14-residue copolymer of glycine and alanine gave brilliant ebv-specific nuclear staining in the anticomplement immunofluorescence (acif) assay, in line with the original definitio ... | 1984 | 6205400 |
| inhibition of human t lymphocyte function with monoclonal antibodies. | 1984 | 6205713 | |
| idiotypic determinants on human t cells and modulation of human t cell responses by anti-idiotypic antibodies. | the role of idiotypic anti-idiotypic interactions in the regulation of the human t cell response to tetanus toxoid (tt) antigen was examined in three subjects. rabbit anti-idiotypic (anti-id) antisera were raised against igg (fab')2 anti-tt obtained 7 to 10 days after booster immunization with tt. f(ab')2 fragments of rabbit-anti-id igg were used in conjunction with fluorescein-conjugated goat anti-rabbit ig in an indirect immunofluorescence assay to determine the frequency of id-positive cells ... | 1984 | 6206140 |
| immunohistochemical study of nasopharyngeal carcinoma using monoclonal keratin antibodies. | nasopharyngeal carcinoma (npc) provides a unique opportunity to evaluate distinctive epidemiologic features and a possible etiologic relationship with epstein-barr virus (ebv) in human malignancy. the lack of a uniformly accepted pathologic classification for npc has limited the application of this data, although the world health organization (who) developed a classification that may solve this problem. monoclonal keratin antibodies were used for staining of npc for evaluation of its assistance ... | 1984 | 6207735 |
| [immunologic problems in allogenic and autologous bone marrow transplantation]. | 1984 | 6208106 | |
| the detection of hla-dr, mb and mt determinants on purified class ii molecules by inhibition of microcytotoxicity. | an assay has been developed which makes it possible to determine the hla allospecificities carried by molecules in purified fractions of detergent lysates from ebv-transformed human lymphocytes. it is based on inhibition of the standard microlymphocytotoxic test used for identifying hla class i and ii antigens with alloantisera. soluble cell membrane products from ebv-transformed cell lines homozygous for the hla region gave specific inhibition of standard typing antisera. the test requires prei ... | 1984 | 6208285 |
| antiviral and antiproliferative effects of interferons delivered via monoclonal antibodies. | we present in vitro experiments demonstrating the feasibility of specifically targeting ifns by coupling to monoclonal antibodies (mabs). human interferon alpha (huifn-alpha), both natural and recombinant, were coupled to epstein-barr virus (ebv) membrane antigen (ma) specific mabs and shown to retain full antiviral and antiproliferative activities when tested on several human cell lines. a series of in vitro pulsing experiments demonstrated that a minimum amount of mab-delivered ifn exerts pron ... | 1984 | 6208296 |
| identification and characterization of a cellular protein that cross-reacts with the epstein-barr virus nuclear antigen. | a 62,000-dalton (62k) cell protein reacts with antisera to the 72k polypeptide of the epstein-barr virus nuclear antigen (ebna) in immunoblots. this protein was initially detected in ebna-negative as well as ebna-positive cell lines with anti-ebna-positive human sera. a monoclonal antibody raised against the 72k ebna and an antiserum from a rabbit immunized with the glycine-alanine domain of ebna also reacted with the cellular protein. the cellular protein was partially purified from epstein-bar ... | 1984 | 6208381 |
| rapid expansion of allospecific cytotoxic t cell clones using nonspecific feeder cell lines without further addition of exogenous il2. | the availability of large numbers of functionally defined cloned t cells would facilitate biochemical and molecular studies and might be a prerequisite for in vivo immunotherapy with cytotoxic lymphocytes. exogenous interleukin-2 (il-2) is generally considered to be the most important medium supplement for the growth of human t cells or cloned t cells. we have studied the role of particular feeder cells or stimulator cells, or both, on the expansion of a panel of cytotoxic and noncytotoxic human ... | 1984 | 6208657 |
| [structural organization of the epstein-barr virus genome]. | the properties and structure of epstein-barr virus (ebv) dna are described. the restriction endonuclease maps of the viral genome are presented. differences in the structure of dnas from various subtypes of ebv and their influence on the phenotype status are discussed. | 1984 | 6209094 |
| migration inhibition factor secreting human t-cell lines reactive to ppd: a study of their antigen specificity, mhc restriction and the use of epstein-barr virus-transformed b-cell lines as requirement for antigen-presenting cells. | two ppd-reactive t-cell lines and two clones derived from them have been characterized. the lines were maintained for a period of 10-12 weeks in i1-2 containing medium. the clones were derived from the uncloned lines by the limiting dilution method and maintained in culture for 12 weeks. the cloning efficiency was 1%. both the cloned and the uncloned lines were highly reactive to tuberculin in a proliferation assay and produced migration inhibition factors following antigenic stimulation. both t ... | 1984 | 6209204 |
| human t-cell hybrids with hla-restricted proliferative response to epstein-barr virus-infected b cells. | human t-cell hybrids were constructed from an hgprt-negative mutant of the acute lymphoblastoid leukaemia cell-line cem and an uncloned population of t cells from donor sw (sw-t; partner cell) known to have a strong specificity for the autologous epstein-barr virus (ebv)-transformed b cell, swebv. the resulting hybrids, 1a9, 1d12 and 2c8, were shown not to be cytotoxic to swebv, nor did they have natural killer-like (nk) activity. however, when presented with the target swebv in a mixed lymphocy ... | 1984 | 6209205 |
| u2 region of epstein-barr virus dna may encode epstein-barr nuclear antigen 2. | sequence analysis of the u2 regions of the b95-8 and ag876 epstein-barr virus (ebv) isolates reveals divergence within a long open reading frame previously identified as encoding 1.5 kilobases of the 3' end of a viral rna expressed in latently infected, growth-transformed, b-lymphocyte cell lines. differences among ebv isolates within the u2 open reading frame are shown to correlate with differences in an ebv nuclear antigen, ebna2. b95-8, w91, raji, cherry, and lamont ebv isolates have similar ... | 1984 | 6209719 |
| [induction of baboon herpes virus antigens in producing lymphoblastoid cells]. | treatment of lymphoid 594s/f9 cells with 12-o-tetradecanoylphorbol-13-acetate (tpa), cycloheximide (ch) has no effect on cell proliferation. a reversible blocking of the cellular dna and rna synthesis is observed. repeated treatment with tpa, iododeoxyuridine (idu) or sodium butyrate (sb) causes more pronounced changes in cell metabolism, which sharply decreases the salt sensitive dna polymerase activity. simultaneously the activity of the virus-induced ammonium sulphate resistant dna polymerase ... | 1984 | 6210192 |
| phenotypic markers and functional characteristics of t lymphocyte clones from cerebrospinal fluid in multiple sclerosis. | we have recently developed a technique for direct expansion of human t lymphocyte clones from cerebrospinal fluid (csf) of patients with acute infections of the central nervous system (cns). in the present study, t lymphocyte clones were established directly from the csf of 4 patients with multiple sclerosis by limiting dilution in the presence of t cell growth factor and irradiated feeder cells. in 3 patients the csf was obtained during an exacerbation of their disease. cloning efficiencies ran ... | 1984 | 6210303 |
| autoantibodies reactive with small ribonucleoprotein antigens: a convergence of molecular biology and clinical immunology. | autoantibodies to nuclear antigens (ana) occur in patients with systemic lupus erythematosus (sle) and other multisystem autoimmune diseases. although heterogeneous, there are 2 major groups, autoantibodies to dna and autoantibodies to non-dna antigens, the latter including anas to the soluble or "extractable nuclear antigens" (ena). this review discusses those enas which are ribonucleoproteins (rnps) consisting of small rna molecules (80-400 nucleotides) attached to non-histone proteins: these ... | 1984 | 6210367 |
| human helper t cell factor(s). iv. demonstration of a human late-acting b cell differentiation factor acting on staphylococcus aureus cowan i-stimulated b cells. | at least two distinct b cell stimulatory factors (bsf) were found to be involved in the differentiation of staphylococcus aureus cowan i (sac)-stimulated human b cells to igg-producing cells. a factor tentatively called b cell differentiation factor i (bcdf i) was found in one fraction, and a second factor, bcdf ii was found in another fraction. the bcdf i fraction alone induces igg-production in sac-stimulated b cells, but the bcdf ii fraction does not. the bcdf ii fraction enhances igg product ... | 1984 | 6234360 |
| characterization of the la (ss-b) antigen from several mammalian sources. | the la or ss-b antigen is associated with rheumatic diseases, systemic lupus erythematosus, and sjogren's syndrome, and is part of a larger ribonucleoprotein complex. immunoaffinity chromatography allowed for the efficient separation of the la antigen from the bulk of the cellular proteins, with a minimum of protease exposure. protein blot analysis of the affinity-isolated material indicated a major immunoreactive polypeptide of 50,000 m.w. a comparison of this antigen in a number of mammalian s ... | 1984 | 6235288 |
| human b-like lymphoblastoid cell lines obtained by long-term culture of normal spleen leukocytes. | b-like lymphoblastoid cell lines were obtained by long-term culture of human spleen leukocytes in rpmi 1640 medium containing human plasma fraction instead of whole foetal calf serum. these cell lines, which did not form e-rosettes had membrane immunoglobulins, and expressed epstein-barr virus antigens. most synthesized intracytoplasmic immunoglobulins and were shown to be diploid and to remain so after subcultures. all produced interferon upon induction with sendai virus. | 1984 | 6235882 |
| [severe and fatal non-a non-b hepatitis in 4 young and children]. | four cases on non-a, non-b acute hepatitis have been observed in infants of the same family. no evidence of hepatitis a or b (hbs-ag, anti-hbs antibodies, hbe-ag, anti-hbe and anti-hbc antibodies) could be detected in sera. virological investigations showed no evidence of cytomegalovirus, epstein-barr virus, infection, etc. the two younger infants developed disseminated intravascular coagulation and died. the other two recovered without sequelae. | 1984 | 6236441 |
| inverse correlation between age related abnormalities of t-cell immunity and circulating thymosin alpha 1 levels in haemophilia a. | t-cell immunity and serum levels of thymosin alpha 1, beta 2-microglobulin, circulating immune complexes, serum immunoglobulin levels, antibodies to hepatitis surface or core antigen, and to cytomegalovirus, and epstein-barr virus were investigated in 51 patients with haemophilia a ranging in age from 2 to 52 years. all patients had received commercial u.s. lyophilized concentrates of antihaemophilic factor (ahf). the mean helper/cytotoxic-suppressor (okt4/okt8) ratio of 11 pre-adolescents (1.6 ... | 1984 | 6236839 |
| diagnostic importance of immunological markers in lymphoma involving the central nervous system. | in an elderly woman, lymphoma involved the central nervous system and unusual nonlymphoreticular sites. cerebrospinal fluid cell surface marker studies revealed a clone of malignant b cells. studies of peripheral blood demonstrated a systemic disturbance of immune regulation similar to epstein-barr virus infections and the acquired immunodeficiency syndrome. | 1984 | 6240961 |
| hypogammaglobulinaemia in systemic lupus erythematosus: report of a case with evidence for spontaneously activated t suppressor cells. | a patient with hypogammaglobulinaemia associated with systemic lupus erythematosus (sle), a healthy hla-a, b, c, d-identical and mixed lymphocyte culture (mlc)-negative sibling, and two mutually hla-a, b, c, d-identical siblings were investigated. blood mononuclear cells from the patient contained a high proportion of t lymphocytes with the suppressor/cytotoxic phenotype, and in vitro no development of ig-secreting cells was observed in response to pokeweed mitogen (pwm) or to epstein-barr virus ... | 1984 | 6241859 |
| [epstein-barr virus early antigen induction in raji cells by chinese medicinal herbs]. | 1984 | 6242369 | |
| human helper t cell factor(s). iii. characterization of b cell differentiation factor i (bcdf i). | staphylococcus aureus cowan i (sac) induced proliferation of human b cells but did not lead the cells to differentiate to immunoglobulin (ig)-producing cells. the differentiation of sac-stimulated b cells to igg-producing cells was totally dependent on t cells or t cell factor, designated b cell differentiation factor i (bcdf i). bcdf i exerted its effect on b cells between 48 and 72 hr after the initiation of the culture, at which time the 3h-tdr uptake of sac-stimulated b cells had already rea ... | 1984 | 6228579 |
| modulation of human b cell immunoglobulin secretion by the c3b component of complement. | the human c3b component of complement was found to inhibit the differentiation of human b lymphocytes into immunoglobulin-secreting cells in vitro. pokeweed mitogen (pwm)-induced plaque-forming cell (pfc) responses were inhibited by c3-coated zymosan particles and by purified human c3b. c3b inhibited the pwm-driven responses in a dose-dependent fashion, and it was necessary for c3b to be present in the early phases of the cultures. c3b acted directly on b cells rather than on helper t cells beca ... | 1984 | 6228593 |
| humoral and cellular immunity in patients with nasopharyngeal carcinoma. | clinical, serological and lymphocyte studies were done on 435 patients with biopsy proved anaplastic nasopharyngeal carcinoma (npc) in various clinical status, at the national taiwan university hospital, from january 1980 through june 1983. studies on 134 normal control were also done. using immunofluorescent antibody method, seropsitive rates of the antibody titers against viral capsid antigens (vca) and early antigens (ea) of epstein-barr (eb) virus were 70.8%-100% for anti-vca/igg titers (gre ... | 1984 | 6086249 |
| [immunoregulation and the formation of rheumatoid factors. review]. | 1984 | 6086478 | |
| presence of retrovirus in the b95-8 epstein-barr virus-producing cell line from different sources. | the b95-8 cell line, a widely used source of highly transforming epstein-barr virus (ebv), obtained from the laboratory of origin, harbored an infectious retrovirus. this retrovirus generally resembled the type d retroviruses structurally and developmentally and like the type d retroviruses preferred mg2+ to mn2+ in its rna-directed dna polymerase reaction. evidence for the presence of retrovirus was found in b95-8 cultures from two other sources within the united states, either by assay for pol ... | 1984 | 6086497 |
| variation in expression of mouse erythrocyte receptors on epstein-barr virus-induced b-cell lines. | to explain the variation in the percentage of mouse erythrocyte rosette-forming cells (merfc) during culture of epstein-barr virus (ebv)-induced b-cell lines, we provide evidence that (i) there is an altered expression of mouse red blood cell (mrbc) receptors on cell line cells during the mitotic cycle, and (ii) putative receptor-negative cells are capable of de novo synthesis of the receptor, and passively adsorbing receptor shed from receptor-positive cells. | 1984 | 6086504 |
| human lymphoblastoid cell lines established from peripheral blood lymphocytes secreting immunoglobulins directed against herpes simplex virus. | peripheral blood lymphocytes (pbl) from genital herpes simplex virus type 2 (hsv-2) patients were transformed with epstein-barr virus. using conditions optimized for successful transformation, two lymphoblastoid cell lines (lcl) have been established, which secrete immunoglobulin g (igg) to hsv. these results suggest that pbl from patients suffering recurrent herpes virus infections can be used to establish lcl secreting hsv antigen specific human immunoglobulins. | 1984 | 6086505 |
| characterization of african green monkey b-cell lines releasing an adult t-cell leukemia-virus-related agent. | eight lymphoblastoid cell lines were established from the peripheral blood of individual african green monkeys (agm). the agm-2206 line grew out spontaneously. the others - agm-6, 7, 8, 10, 12, 13, and 16 - were obtained after infection of peripheral agm lymphocytes with cell-free culture supernatant of agm-2206. all lines contained, and were probably transformed by, agm-ebv. moreover, they expressed immunoglobulins but lacked the leu l t-cell marker. thus they were b cells. since a high percent ... | 1984 | 6086534 |
| evaluation of dna homology of marek's disease virus, herpesvirus of turkeys and epstein-barr virus under varied stringent hybridization conditions. | marek's disease virus (mdv) showed only 0.3-0.6% homology in dna sequence with herpesvirus of turkeys (hvt) at tm-24.4 degrees c in spite of its antigenic similarity to the latter. southern blot hybridization under stringent conditions showed that the homology between mdv and hvt is located in the restricted portion of these viral genomes. at tm-49.6 degrees c, which permits the detection of homology with one base mismatch in three between the mdv and hvt dnas, sequences with weak homology were ... | 1984 | 6086596 |
| further characterization of igm antibodies against maternal alloreactive t cells produced by cloned epstein barr virus-transformed cord b cells. | the mother obviously recognizes the fetus as an antigen, and the fetus is protected from the maternal immunologic attacks by eliciting anti-alloreactive t cell antibodies (t lymphocytotoxic human fetal antibody; tlfa). tlfa contains several antibodies against the maternal cells. it is thus necessary for further understanding of tlfa to obtain a single antibody from ebv-transformed cord b cells. ebv-transformations were performed in 28 cord b cell samples, and 16 cell lines were established. anti ... | 1984 | 6086753 |
| serum-free medium for generation and propagation of functional human cytotoxic and helper t cell clones. | a serum-free lymphocyte culture medium is described in which serum is replaced by bovine serum albumin, transferrin, insulin, ethanolamine and a mixture of saturated and unsaturated fatty acids (linoleic acid, oleic acid and palmitic acid). in this serum-free medium proliferative and cytotoxic responses induced in mixed lymphocyte culture were comparable with those obtained in medium containing serum. antigen-specific cytotoxic and helper t cells were isolated and could be propagated in serum-fr ... | 1984 | 6086760 |
| interferons on the transformation of non-t blood mononuclear leukocytes by epstein--barr virus. | the effect of partially purified leukocyte (alpha) and fibroblast (beta)-derived interferons (ifns) and crude immune (gamma) ifn on the transformation of non-t mononuclear leukocytes by the epstein--barr (ebv) virus was studied. the ifn preparations reduced the frequency of cells positive for ebv-specific nuclear antigen but did not significantly prolong the ebv-transformation interval. | 1984 | 6086777 |
| epstein-barr virus infection and immunologic abnormalities. | 1984 | 6086867 | |
| nucleotide sequence of an mrna transcribed in latent growth-transforming virus infection indicates that it may encode a membrane protein. | the most abundant epstein-barr virus mrna in a latently infected cell line, ib4, established by in vitro growth transformation with virus, was a 2,8-kilobase rna encoded by largely unique dna near the right end of the genome. the rna was transcribed from right to left, and two introns were spliced out. this region of the genome was sequenced, and the exons of the rna were identified by s1 analysis of dna-rna hybrids and primer extension. the first start codon in the rna was 40 nucleotides from i ... | 1984 | 6086953 |
| [a case of castleman lymphoma (plasma cell type) in retroperitoneum with high titer of anti eb virus antibodies]. | 1984 | 6086988 | |
| demonstration of terminal deoxynucleotidyl transferase in single cells by indirect immunofluorescence. ii. an examination of specificity. | advantages in the use of indirect immunofluorescence for the identification of terminal transferase (tdt) in single cells may be offset by lack of specificity, as compared to the biochemical assay of the enzyme, especially in analyses of lymphocyte populations. false positive results were obtained in 15/15 tonsillectomy samples and in 9/27 specimens from children with acute lymphoblastic leukemia in remission, perhaps due to antihuman lymphocyte activity in the rabbit heteroantisera which are us ... | 1984 | 6087036 |
| human monoclonal antibodies. | the technology for the production of murine monoclonal antibodies has been refined enormously since its introduction in 1975. however, the technology for generating human monoclonal antibodies has only recently come into its own. in this review, three currently available approaches to the production of human monoclonal antibodies are described. these include the hybridoma technique, based on the fusion of antibody-producing human b lymphocytes with either mouse or human myeloma or lymphoblastoid ... | 1984 | 6087121 |
| dna sequence and expression of the b95-8 epstein-barr virus genome. | the complete (172,282 base pairs) nucleotide sequence of the b95-8 strain of epstein-barr virus has been established using the dideoxynucleotide/m13 sequencing procedure. many rna polymerase ii promoters have been mapped and the mrnas from these promoters have been assigned to the latent or early/late productive virus cycles. likely protein-coding regions have been identified and three of these have been shown to encode a ribonucleotide reductase, a dna polymerase and two surface glycoproteins. | 1984 | 6087149 |
| isolation of htlv-transformed b-lymphocyte clone from a patient with htlv-associated adult t-cell leukaemia. | the human t-cell leukaemia/lymphoma virus (htlv) is an exogenous retrovirus which has been associated with adult t-cell leukaemia/lymphoma (atl). this malignancy of t lymphocytes is endemic to southern japan, the west indies, and to a lesser extent, the middle east, central africa and the southeastern united states. atl cells from patients of diverse geographical origins have been found to be infected with htlv-1 (ref.6). htlv is normally tropic for mature t lymphocytes, especially those express ... | 1984 | 6087161 |
| effects of chemical inducers on the epstein-barr virus cycle in epithelial/nasopharyngeal carcinoma hybrid cell line. | we have studied the induction of epstein-barr virus (ebv)-specific antigens in nasopharyngeal carcinoma hybrid cells by treatment with the tumor promotor 12-0-tetradecanoylphorbol-13-acetate (tpa), hydrogen peroxide (h2o2) and n-butyric acid. the intensity of ebv induction by these drugs was in the order of n-butyric acid----tpa----h2o2. furthermore, the combination of h2o2 or tpa and n-butyric acid acted synergistically on ebv-induced antigens. | 1984 | 6087237 |
| infection of human epithelial cell lines by epstein-barr virus. interaction between epithelial cell and b lymphocyte. | the pathogenesis of nasopharyngeal carcinoma caused by the epstein-barr virus (ebv) has been studied experimentally by infecting monolayer cells derived from human normal nasopharynx with the b-lymphotropic ebv. direct ebv infection of the cell sheets derived from the human nasopharynx resulted in the appearance of ebv-associated nuclear antigen-positive floating cells, suggesting that the monolayer cells have been transformed. since these cells seemed to be of the b-type lymphocyte, it may be a ... | 1984 | 6087238 |
| identification of epstein-barr virus sequences that encode a nuclear antigen expressed in latently infected lymphocytes. | the epstein-barr virus (ebv) bamhi restriction endonuclease fragment k (b95-8 strain) was introduced into a polyoma virus expression vector and used to transfect murine nih 3t3 cells. an ebv-associated nuclear antigen was detected in these cells in an indirect immunofluorescence test using anti-ebv nuclear antigen-positive human sera. these sera recognized a mr 88,000 polypeptide in 3t3 cells transfected with the bamhi fragment k-containing polyoma virus plasmid by radioimmunoelectrophoresis. a ... | 1984 | 6087322 |
| epstein-barr virus receptor of human b lymphocytes is the c3d receptor cr2. | identity of the epstein-barr virus (ebv) receptor with the complement receptor type 2 (cr2) was established in three sets of experiments using the monoclonal antibodies, hb-5 and anti-b2, which recognize a mr 145,000 b-lymphocyte membrane protein that is cr2. first, the rank order for binding of fluoresceinated ebv to four lymphoblastoid cell lines (sb, jy, raji, and molt-4) was identical to the rank order for binding of hb-5 and anti-b2 by analytical flow cytometry. second, pretreatment of cell ... | 1984 | 6087328 |
| expression of a nuclear and a cytoplasmic epstein-barr virus early antigen after dna transfer: cooperation of two distant parts of the genome for expression of the cytoplasmic antigen. | expression of epstein-barr virus (ebv) antigens was studied after transfection of cloned ebv dna fragments into baby hamster kidney (bhk) cells. a set of seven widely overlapping clones covering the whole genome of the non-defective epstein-barr virus strain m-aba was used for transfection. transfer of the cosmid clones into bhk cells resulted in expression of two distinct antigens, as revealed by indirect immunofluorescence using human anti-ebv sera. staining with human sera of different reacti ... | 1984 | 6087334 |
| no evidence for differences in the epstein-barr virus genome carried in burkitt lymphoma cells and nonmalignant lymphoblastoid cells from the same patients. | epstein-barr virus (ebv), although not an indispensable factor for the development of burkitt lymphoma, is apparently associated with the 20-fold higher incidence of the disease in equatorial africa compared to the incidence in other parts of the world. to determine whether different ebv subtypes are associated with the appearance of the malignant phenotype, we have compared the ebv genomes carried in the burkitt tumor cells with those carried in the nonmalignant lymphoblastoid cells from the sa ... | 1984 | 6087353 |
| infection of b lymphocytes by a human herpesvirus, epstein-barr virus, is blocked by calmodulin antagonists. | epstein-barr virus (ebv) is a human herpesvirus that selectively binds to and infects human b lymphocytes (b cells). in the studies presented here, we found that several phenothiazines, including trifluoperazine, chlorpromazine, prochlorpromazine, and promethazine, blocked ebv infectivity of isolated adult human b cells as measured either by outgrowth of transformed cell colonies or by [3h]thymidine incorporation. trifluoperazine, chlorpromazine, and prochlorpromazine were equally effective with ... | 1984 | 6087356 |
| regions of the ebv genome involved in latency and lymphocyte immortalization. | 1984 | 6087408 | |
| ebv expression in human non-lymphoid hematopoietic cell lines. | 1984 | 6087409 | |
| hodgkin's disease: the remaining challenge. | 1984 | 6087413 | |
| the changing faces of ebv research. | 1984 | 6087416 | |
| infection of normal human thymic epithelial cells by epstein-barr virus (ebv) following implantation of ebv receptors. | normal human thymic epithelial cells cannot be infected and transformed by epstein-barr virus (ebv). measurements using fluorescein-labelled ebv and cytofluorograph revealed that the cells do not have receptors for the virus. following transplantation of functional ebv receptors onto epithelial cell membranes, however, the cells were infected with ebv and expressed ebv-determined nuclear antigen (ebna). no ebv associated antigens characteristic of the lytic cycle of the virus were detected. this ... | 1984 | 6087504 |
| epstein-barr viral hepatitis: an unusual case and review of the literature. | we report a patient with icteric hepatitis and abdominal pain caused by epstein-barr virus in the absence of other common features of infectious mononucleosis. the peak alanine aminotransferase was 289 iu/i. hemolytic anemia and urinary retention complicated the patient's course. patients with infectious mononucleosis commonly have hepatic involvement but isolated symptomatic hepatitis is unusual. although rare cases of liver failure have been reported, there is no evidence that epstein barr vir ... | 1984 | 6087655 |
| fatal epstein-barr-virus-associated proliferation of donor b cells after treatment of acute graft-versus-host disease with a murine anti-t-cell antibody. | two patients with acute leukemia were treated with chemoradiotherapy and allogeneic bone marrow transplantation. despite the prophylactic use of methotrexate after grafting, both patients developed severe graft-versus-host disease that was refractory to treatment with methylprednisolone. the graft-versus-host disease was then treated with a monoclonal antibody, 64.1, that reacts with a p19 antigen on human t cells. the disease responded dramatically to this treatment, but both patients subsequen ... | 1984 | 6087703 |
| ultrastructural investigations on two lymphoid cell lines from niemann-pick disease type b. | lymphoid cell lines (lcl) were established by epstein-barr virus (ebv) transformation of blood b-lymphocytes from two different patients affected with niemann-pick disease (npd) type b. those lines were severely deficient in sphingomyelinase activity (8% and 10% residual activity). ultrastructural investigations showed in both these lines the presence of numerous osmiophilic, dense and pleiomorphic inclusions characteristic of lysosomal storage (due to the accumulation of amphiphilic lipids) sim ... | 1984 | 6087964 |