Publications

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in situ and laboratory bioassays to evaluate the impact of effluent discharges on receiving aquatic ecosystems.effluents are a main source of direct and often continuous input of pollutants into aquatic ecosystems with long-term implications on ecosystem functioning. therefore, the study of the effects of effluent exposure on organisms, populations or communities within the framework of impact assessment has a high ecological relevance. the aim of this study was to assess the toxicological impact of two effluents, one household wastewater treatment effluent (effluent 1) and one industrial effluent (efflu ...200415312937
effect of naproxen on tail fin regeneration in teleost.in the present investigation we studied the effect of naproxen, a non-steroidal anti-inflammatory drug used for the treatment of rheumatic disease, on the synthesis of the lepidotrichial matrix of the tail fin of the teleost fish cyprinus carpio (carp) during the regeneration process. the lepidotrichial synthesis was observed by standard and polarized light microscopy and by transmission electron microscopy. in general, naproxen at the dose used in the present study did not affect the organizati ...200415311675
trends in two year risk of repeat revascularisation or death from cardiovascular disease after coronary artery bypass grafting or percutaneous coronary intervention in western australia, 1980-2001.to investigate whether, over the 21 year period 1980-2001, there had been a reduction in the risk of repeat revascularisation or death from cardiovascular disease in the cohort of all patients who were treated by coronary revascularisation in western australia.200415310696
trends in coronary artery revascularisation procedures in western australia, 1980-2001.to describe trends in the use of coronary artery revascularisation procedures (carps) and to determine whether or when carp rates will stabilise.200415310694
immunohistochemical distribution of neuropeptide y in the mesencephalon and rhombencephalon of carp, cyprinus carpio l. (cyprinidae: teleostei).the localization of neuropeptide y (npy)-immunoreactive elements was investigated in the mesencephalon and rhombencephalon of carp, cyprinus carpio, by using antisera raised against porcine npy and the immunoperoxidase technique. concurrently, to identify the distribution of npy-immunoreactivity, we developed an atlas of the studied areas based on nissl-stained sections. the npy-immunoreactive (npy-ir) elements were located in many zones of the mesencephalon and rhombencephalon. in the mesenceph ...200415275652
characterization of a new c-type lectin from common carp cyprinus carpio.a new c-type lectin with putative mannose specificity was identified from the expressed sequence tag (est) analysis of cdna library from common carp head kidney (hk), stimulated with concanavalin a and lipopolysaccharide (lps) during a routine est analysis. the full sequence of 627 bp was identified by 5'-rapid amplification of cdna ends. the gene is composed of 146 amino acid residues, including an 18-residue signal peptide for secretion and a single carbohydrate-recognition domain of approxima ...200415261461
decontamination effect of electrolysed nacl solutions on carp.to evaluate the efficacy of electrolysed nacl solutions (ew) for disinfecting bacterial isolates from carp, and the potential application of ew to reducing the bacterial load in whole carp and carp fillets.200415242456
modulative influence of lysozyme dimer on defence mechanisms in the carp (cyprinus carpio) and european sheatfish (silurus glanis) after suppression induced by herbicide roundup.immunomodulation is a commonly used method of prophylaxis in humans and animals. lysozyme dimer (klp-602) was used at a dose of 50 ug/kg b.w. in order to correct the immunosuppression caused by the action of herbicide glyphosate (roundup- monsanto), which was used in a single bath for 10 minutes in a concentration of 100 mg/l of water. the investigations were carried out on 2 species of fish: the carp (cyprinus carpio l.) and european catfish (silurus glanis l.). herbicide glyphosate caused a de ...200415230544
modulation in hepatic and head kidney parameters of carp (cyprinus carpio l.) induced by copper and chitosan.copper is used in treatment mixtures to control fungal diseases in vineyards plants. high concentrations of copper are inducing antioxidant stress in some aquatic ecosystems, and potential bioaccumulation in aquatic organisms has prompted the demand for alternative use of low toxic molecules in culture treatments. chitosan is a biomolecule with antifungal and heavy metal ion chelating properties that may be used as a biopesticide. in this study, we investigate the potential toxicity of chitosan ...200415228950
localization, expression and control of adrenocorticotropic hormone in the nucleus preopticus and pituitary gland of common carp (cyprinus carpio l.).adrenocorticotropic hormone (acth) takes a central role in the hypothalamo-pituitary-interrenal axis (hpi axis), which is activated during stress. acth is produced by the corticotrope cells of the pituitary pars distalis (pd) and is under control of factors from the nucleus preopticus (npo). the distribution of acth in the hypothalamo-pituitary system in common carp (cyprinus carpio l.) was assessed by immunohistochemistry. acth and beta-endorphin immunoreactivity was observed in the acth cells ...200415225128
specific cytotoxic activity generated by mixed leucocyte culture in ginbuna crucian carp. 200415212738
in vitro effects of steroid hormones on igm-secreting cells and igm secretion in common carp (cyprinus carpio).the effects of steroid hormones on in vitro igm-secreting cells (igmsc) and igm secretion by lymphocytes of the lymphoid organs in common carp, cyprinus carpio were examined by elispot and elisa assay, respectively. cells isolated from peripheral blood (pb), spleen and head kidney were cultured for 12, 24 and 48 h either in the absence or in the presence of steroid hormones, i.e. cortisol, testosterone, 11-ketotestosterone (11-kt), and estradiol-17beta (e(2)) at doses of 1, 10 and 100 ng/ml. cor ...200415212735
hydrodynamics of burst swimming fish larvae; a conceptual model approach.burst swimming of fish larvae is analysed from a hydrodynamic point of view. a picture of the expected flow pattern is presented based on information in literature on unsteady-flow patterns around obstacles in the intermediate reynolds number region. it is shown that the acceleration stage of burst swimming under restricted conditions can be treated as a frictionless impulsive motion. the stream pattern resulting from this motion is presented and the efficiency of locomotion during the accelerat ...200415207478
molecular cloning of proopiomelanocortin cdna and multi-tissue mrna expression in channel catfish.channel catfish (ictalurus punctatus) proopiomelanocortin (pomc) cdna was cloned to investigate its structure, evolution, and expression in different tissues. pomc is an important gene in the hypothalamus-pituitary-adrenal axis, the main mediator of the stress response. pomc gene was isolated from a pituitary cdna library and nucleotide sequence was determined. pomc cdna is composed of 1164 nucleotides with a 639 nucleotide open reading frame encoding a protein of 212 amino acids. catfish pomc p ...200415201069
effect of cadmium on blood plasma biochemistry in carp (cyprinus carpio l.). 200415199987
effect of cadmium on hematological indices of common carp (cyprinus carpio l.). 200415199986
sequences of transgene insertion sites in transgenic f4 common carp. 200415198197
hematologic reference intervals for koi (cyprinus carpio), including blood cell morphology, cytochemistry, and ultrastructure.hematologic data are used routinely in the health care of humans and domestic mammals. similar data for fish are largely fragmentary or have not been collected.200415195266
epithelio-reticular cell thymoma in carp, cyprinus carpio l: an ultrastructural study. 200415189377
response of cyprinus carpio (linn) to sublethal concentration of cypermethrin: alterations in protein metabolic profiles.freshwater fish, cyprinus carpio were exposed to sublethal concentration (1.2 microg l(-1)) of cypermethrin for 6, 12, 24 and 48 h to analyze various parameters of protein metabolism in functionally different tissues. total, structural and soluble proteins showed decrement; where as free amino acids and the activities of protease, aspartate aminotransferase and alanine aminotransferase significantly increased in cypermethrin exposed fish. interestingly, ammonia content decreased but urea and glu ...200415183996
purification and functional assessment of c3a, c4a and c5a of the common carp (cyprinus carpio) complement.promotion of inflammatory response is an important role of the complement system, but this kind of function is poorly documented for the lower vertebrates. here we report chemotactic activity of purified anaphylactic fragments derived from the complement components c3, c4 and c5 of the common carp. the purified anaphylatoxins are two c5a-desarg peptides derived from the c5-i isotype, an intact form and a desarg form of c4a from c4-2 isotype, and an intact form and a desarg form of c3a from c3-h1 ...200415183031
ultrastructural identification of a herpes-like virus infection in common carp cyprinus carpio in korea.we report on a herpes-like virus, which was found to be associated with mass mortality of common carp cyprinus carpio for the first time in korea in 1998. the external signs of infection in moribund fish were darkened coloration and severe branchial necrosis in the gill. transmission electron microscopy revealed the presence of herpes-like viruses in spleen tissue. infected spleen cells showed hypertrophied nuclei and degeneration. numerous nucleocapsids of about 82 nm in diameter were found wit ...200415584424
a novel serine protease complexed with alpha2-macroglobulin from skeletal muscle of lizard fish (saurida undosquamis).a novel fish muscle serine protease named muscle soluble serine protease (mssp) was purified from the soluble fraction of lizard fish (saurida undosquamis: synodontidae) muscle by ammonium sulfate fractionation followed by four steps of column chromatographies. in native-page, the purified enzyme appeared as a single band with an estimated mol. mass of approximately 380 kda by gel filtration. in sds-page under reducing conditions, the purified enzyme migrated as two protein bands at 110 and 100 ...200415581796
annual evolution of fatty acid profile from muscle lipids of the common carp (cyprinus carpio) in madagascar inland waters.annual evolution of muscle lipids fatty acid (fa) from common carp (cyprinus carpio) has been determined in 2001 through monthly samplings in the reserve pond of sisaony (sis series) and itasy lake (ita series) of the madagascar highlands. total lipids from muscle were extracted and quantified according to the bligh and dyer method. fa identification was performed by gc-ms of fa methyl esters and fa pyrrolidides and led to the identification of 41 fa; routine analyses of fa were made by capillar ...200415563217
[cloning and expression analysis in mature individuals of salmon gonadotropin-releasing hormone (sgnrh) gene in common carp].two types of complementary dnas (cdna) encoding the precursor of salmon gonadotropin-releasing hormone (sgnrh, [trp7, leu8] gnrh) are cloned and sequenced from common carp brain using rapid amplification of cdna ends (race). the two cdnas are referred to sgnrh cdna1 and cdna2, and the full-length fragment of cdna1 and cdna2 were 393 and 478 bp, respectively. two sgnrh cdnas contain an open reading frame of 285 bp, which encodes the sgnrh precursor including 94 amino acid residues. the sgnrh prec ...200415552041
coping with cold: an integrative, multitissue analysis of the transcriptome of a poikilothermic vertebrate.how do organisms respond adaptively to environmental stress? although some gene-specific responses have been explored, others remain to be identified, and there is a very poor understanding of the system-wide integration of response, particularly in complex, multitissue animals. here, we adopt a transcript screening approach to explore the mechanisms underpinning a major, whole-body phenotypic transition in a vertebrate animal that naturally experiences extreme environmental stress. carp were ex ...200415550548
temperature and the expression of myogenic regulatory factors (mrfs) and myosin heavy chain isoforms during embryogenesis in the common carp cyprinus carpio l.embryos of the common carp, cyprinus carpio l., were reared from fertilization of the eggs to inflation of the swim bladder in the larval stage at 18 and 25 degrees c. crna probes were used to detect transcripts of the myogenic regulatory factors myod, myf-5 and myogenin, and five myosin heavy chain (myhc) isoforms during development. the genes encoding myf-5 and myod were switched on first in the unsegmented mesoderm, followed by myogenin as the somites developed. myf-5 and myod transcripts wer ...200415531645
comparative and evolutionary analysis of the cytochrome b sequences in cyprinids with different ploidy levels derived from crosses.the mitochondrial cyt b genes in the allotetraploid and triploid crucian carp as well as triploid common carp were isolated and completely sequenced. their dna sequences were compared with those derived from the cyt b genes of the red crucian carp, japanese crucian carp, and common carp with mega 1.0 software. phylogenetic analysis revealed the sister relationships between allotetraploid and diploid red crucian carp, between the triploid crucian carp and diploid japanese crucian carp, and betwee ...200415521428
disparate opinions regarding indications for coronary artery revascularization before elective vascular surgery.despite consensus guidelines, the optimal strategy for preoperative cardiac risk management among patients scheduled for major noncardiac surgery remains controversial. this study assesses current opinion about the role of preoperative coronary revascularization for patients with coronary artery disease scheduled for elective vascular surgery. thirty-one practicing cardiologists recruited from 4 different regions reviewed case records, imaging tests, and coronary angiograms of 12 patients with c ...200415518605
determination of a bound musk xylene metabolite in carp hemoglobin as a biomarker of exposure by gas chromatography-mass spectrometry using selected ion monitoring.musk xylene (mx) is widely used as a fragrance ingredient in commercial toiletries. identification and quantitation of a bound 4-amino-mx (amx) metabolite was carried out by gas chromatography-mass spectrometry (gc-ms) with selected ion monitoring (sim). detection of amx occurred after the cysteine adducts in carp hemoglobin (hb), derived from the nitroso metabolite, were released by alkaline hydrolysis. the released amx metabolite was extracted into n-hexane. the extract was preconcentrated by ...200415516317
[studies on the ploymorphic of sperm of f2 hybrids of red crucian carp x common carp].athe ultrastructures of the sperm of f2 hybrids of red crucian carp x common carp were studied by using scanning and transmission electron microscope. the sperm of the f2 hybrids consisted of head, mid-piece and tail. there was no acrosome at the anterior end of the nuclears, whereas there was a vesicle. the results revealed that there existed obviously ploymorphic in the sperm of f2 hybrids. in the water-like semen from males of f2 hybrids, different sizes of the head of the sperm including hap ...200415511069
fumonisin b1 causes multiple lesions in common carp (cyprinus carpio).year-1 carp were fed ratios containing 100mg/kg and 10 mg/kg of added fumonisin b1 for 42 days. the experimental and control fish were examined clinically during the experiment and at the end all fish were necropsied and histological changes recorded. blood vessels, liver, exocrine and endocrine pancreas, excretory and haematopoietic kidney, heart and brain were sensitive both to 100 and 10mg/kg of fb1 in the diet and the rodlet cell (rc) frequency was considerably increased in and around damage ...200415503536
carbonic anhydrases: current state of the art, therapeutic applications and future prospects.carbonic anhydrases (cas, ec 4.2.1.1) are wide-spread enzymes, present in mammals in at least 14 different isoforms. some of these isozymes are cytosolic (ca i, ca ii, ca iii, ca vii, ca xiii), others are membrane-bound (ca iv, ca ix, ca xii and ca xiv), ca v is mitochondrial and ca vi is secreted in the saliva and milk. three cytosolic acatalytic forms are also known (carp viii, carp x and carp xi). the catalytically active isoforms, which play important physiological and patho-physiological fu ...200415499993
impact of some chlorinated pesticides on the haematology of the fish cyprinus carpio and puntius ticto.the effects of pesticides on blood characteristics and histological changes in erythrocytes of the fish species cyprinus carpio and puntius ticto were studied. the fishes were exposed to sub lethal concentrations of different chlorinated pesticides namely aldrin, dieldrin, ddt, bhc and chlordane for 10, 20 and 30 d in continuous flow-through test. the lc50 values were calculated based on acute toxicity tests and the sublethal doses were arrived at for chronic bioassay studies. results showed an ...200415495970
[polymorphism in intron 2 of crucian carp gh i gene].high length and nucleotide polymorphisms in intron2 of gh i gene were detected in 162 individuals,which were from seven wild crucian carp colonies, two goldfish colonies and one fangzheng crucian carp colony. using denaturating polyareylamide gel electrophoresis (dpage) and single-strand conformation polymorphism (sscp), seven length variants and 15 haplotypes were identified in these fishes. the length types and haplotypes diversity was 4.32% and 9.26%, respectively. sequence analysis of the 15 ...200415490876
elevated dna-protein cross-links in red blood cells of german carp, cyprinus carpio, from the lower passaic river, new jersey, usa. 200415669735
noise emission during the first powerboat race in an alpine lake and potential impact on fish communities.in order to assess the effects of high-speed boating on fish communities, noise levels were measured during the first class 1 powerboat race on the austrian lake traunsee. the noise spectra were compared to natural ambient noise and hearing abilities of four native fish species. sound pressure levels (spls) were significantly elevated during the training heats and the race compared with natural levels, reaching up to 128 db re 1 micropa (instantaneous spl) at a distance of 300 m to the powerboat ...200415658729
the strengths of in vivo magnetic resonance imaging (mri) to study environmental adaptational physiology in fish.adaptational physiology studies how animals cope with their environment, even if this environment is subject to permanent fluctuations such as tidal or seasonal variations. aquatic organisms are generally more prone to be exposed to osmotic, hypoxic and temperature challenges than terrestrial animals. some of these challenges are more restraining in an aquatic environment. to date, very few studies have used in vivo magnetic resonance imaging (mri) to uncover the physiological mechanisms that re ...200415614515
reproductive responses of common carp (cyprinus carpio) exposed in cages to influent of the las vegas wash in lake mead, nevada, from late winter to early spring.the las vegas wash (lw) delivers tertiary-treated municipal wastewater effluent, nonpotable shallow groundwater seepage, and runoff from the urbanized las vegas valley to las vegas bay (lx) of lake mead. to investigate the potential for contaminants in lw influent to produce effects indicative of endocrine disruption in vivo, adult male and female common carp (cyprinus carpio) were exposed in cages for 42-48 d at four sites in lake mead: lw, lx, and two reference locations in the lake. end point ...200415597896
calcium-binding protein caldendrin and camkii are localized in spinules of the carp retina.calcium-binding proteins translate the influx of ca(2+) at excitatory synapses into spatiotemporal signals that regulate a variety of processes underlying synaptic plasticity. in the fish retina, the synaptic connectivity between photoreceptors and horizontal cells undergoes a remarkable plasticity, triggered by the ambient light conditions. with increasing light, the synaptic dendrites of horizontal cells form numerous spinules that are dissolved during dark adaptation. the dynamic regulation o ...200415389610
loss of filament-forming ability of myosin by non-enzymatic glycosylation and its molecular mechanism.carp and scallop myosin and their subfragments (s-1 and rod) were reacted with glucose to investigate the effect of non-enzymatic glycosylation on the functionality of myosin. the filament-forming ability of the myosin rod diminished with the progress of non-enzymatic glycosylation and myosin became soluble in 0.1 m nacl. the inhibition of the self-assembly of myosin molecules occurred chemically as a result of the increase in negative charge repulsion among myosin molecules and, further, physic ...200415388325
immunology-related perturbations induced by copper and chitosan in carp (cyprinus carpio l.).copper is used in treatment mixtures to control fungal diseases in vineyards. its concentrations are relatively high in some aquatic ecosystems, and the main problem observed in this study was the antioxidant stress induced by this heavy metal. copper toxicologic effects in aquatic organisms have prompted the demand for alternative use of low-toxicity molecules in culture treatments. chitosan is a polymer with antifungal property similar to copper and may be an interesting biopesticide. thus, it ...200415386131
characterization of retinoic acid neuromodulation in the carp retina.visual sensation in vertebrates starts with the isomerization of 11-cis retinaldehyde into all-trans retinaldehyde. aldehyde dehydrogenases, present in the pigment epithelium and some retinal cells, convert all-trans retinaldehyde into all-trans retinoic acid (at-ra). evidence in the retina and the hippocampus has accumulated, showing that at-ra, besides being a morphogenetic factor, also acts as a neuromodulator. in mature retina, at-ra affects visual processing by acting on gap junctional cond ...200415378613
dietary accumulation and metabolism of polybrominated diphenyl ethers by juvenile carp (cyprinus carpio).polybrominated diphenyl ethers (pbdes) are hydrophobic organic contaminants with properties and nomenclature similar to polychlorinated biphenyls (pcbs). while much information is available on the bioaccumulation and pharamcokinetics of pcbs, little information is available on pbdes. in this study, juvenile carp were exposed to a diet spiked with a cocktail of four bde congeners (2,4,4'-tribromode [bde 28], 2,2',4,4'-tetrabromode [bde 47], 2,2',4,4',5-pentabromode [bde 99], and 2,2',4,4',5,5'hex ...200415352483
xenobiotic substances such as pcb mixtures (aroclor 1254) and tbt can influence swimming behavior and biotransformation activity (gst) of carp (cyprinus carpio).different groups of carp were treated with polychlorinated biphenyl (pcb) or tributyltin (tbt), and possible effects of the chemicals on the swimming behavior of the carp were examined using the behavioquant system. by evaluating quantitative behavioral parameters of the animals, it became evident that exposure to high concentrations of chemicals (organotin, 7 microg l(-1), or polychlorinated biphenyl, 22 microg l(-1)) severely affected the carp, causing a significant change in their swimming sp ...200415352262
the reserpine effects on the gonadotrophic cells of the male common carp cyprinus carpio (osteichtyes: cyprinidae).the secretion of gonadotropins (gth) in goldfish and carp, is stimulated by gth-releasing hormone (gnrh) and is inhibited by dopamine. studies with antidopaminergics have demonstrated to be effective in order to stimulate the spermiation and the ovulation in different species of teleosts. the reserpine, a drug that deplets the dopamine, has shown to stimulate the spermiation in the common carp. we report here, the effects of reserpine on the number and volume of gonadotrophic cells of the common ...200417357409
regulation of action potential duration under acute heat stress by i(k,atp) and i(k1) in fish cardiac myocytes.the mechanism underlying temperature-dependent shortening of action potential (ap) duration was examined in the fish (carassius carassius l.) heart ventricle. acute temperature change from +5 to +18 degrees c (heat stress) shortened ap duration from 2.8 +/- 0.3 to 1.3 +/- 0.1 s in intact ventricles. in 56% (18 of 32) of enzymatically isolated myocytes, heat stress also induced reversible opening of atp-sensitive k+ channels and increased their single-channel conductance from 37 +/- 12 ps at +8 d ...200414592934
maintained cardiac pumping in anoxic crucian carp.like most vertebrates, humans die within minutes when deprived of molecular oxygen (anoxia), in part because of cardiac failure. in contrast, some freshwater turtles can survive anoxia for months at low temperatures, but to do so, they drastically suppress cardiac activity and autonomic cardiovascular control. although carassius carassius, the crucian carp, shares this anoxia tolerance, we show that it has a unique ability among vertebrates to retain normal cardiac performance and autonomic card ...200415459381
hypoxic survival strategies in two fishes: extreme anoxia tolerance in the north european crucian carp and natural hypoxic preconditioning in a coral-reef shark.especially in aquatic habitats, hypoxia can be an important evolutionary driving force resulting in both convergent and divergent physiological strategies for hypoxic survival. examining adaptations to anoxic/hypoxic survival in hypoxia-tolerant animals may offer fresh ideas for the treatment of hypoxia-related diseases. here, we summarise our present knowledge of two fishes that have evolved to survive hypoxia under very different circumstances. the crucian carp (carassius carassius) is of part ...200415299034
vertebrate brains at the pilot light.while the brains of most vertebrates are unable to tolerate more than a few minutes of anoxia, some freshwater turtles (trachemys and chrysemys), crucian carp (carassius carassius) and frogs (rana pipens and rana temporaria) can survive anoxia for hours to months. obviously, anoxia tolerance has evolved separately several times and this is also reflected in the divergent strategies these animals utilize to survive without oxygen. the turtles and crucian carp defend their brain atp levels and avo ...200415288600
seasonality of dihydropyridine receptor binding in the heart of an anoxia-tolerant vertebrate, the crucian carp (carassius carassius l.).prolonged anoxia tolerance of facultative anaerobes is based on metabolic depression and thus on controlled reduction of energy-utilizing processes. one proposed survival mechanism is the closing of ion channels to decrease energetic cost of ion pumping (hochachka pw. science 231: 234-241, 1986). to test this hypothesis, the involvement of l-type ca2+ channels in seasonal anoxia tolerance of the vertebrate heart was examined by determining the number of [methyl-3h]pn200-110 (a ligand of l-type c ...200415242827
metabolic responses to low temperature in fish muscle.for most fish, body temperature is very close to that of the habitat. the diversity of thermal habitats exploited by fish as well as their capacity to adapt to thermal change makes them excellent organisms in which to examine the evolutionary and phenotypic responses to temperature. an extensive literature links cold temperatures with enhanced oxidative capacities in fish tissues, particularly skeletal muscle. closer examination of inter-species comparisons (i.e. the evolutionary perspective) in ...200415191230
neurophysiologic detector-a selective and sensitive tool in high-performance liquid chromatography.in the present study neurons from the olfactory system of the fish crucian carp, carassius carassius l. were used as components in an in-line neurophysiologic detector (npd) to measure physiological activities following the separation of substances by high-performance liquid chromatography (hplc). the skin of crucian carp, c. carassius l. contains pheromones that induce an alarm reaction in conspecifics. extra-cellular recordings were made from neurons situated in the posterior part of the media ...200414698234
growth and development of catla (catla catla) fed with different levels of diet containing spirogyra sp.the effect of spirogyra sp. incorporated into diet formulations on the growth and body composition of indian major carp, catla (catla catla) was investigated in a 45 days feeding trial. spirogyra dry powder was mixed with different feed ingredients in different amounts (0%, 10%, 25%, 37% and 40% of the total feed). carps fed with spirogyra demonstrated higher feed conversion ratio. the study also revealed a direct relationship between the amount of spirogyra in the diet, and muscle protein and f ...200415207298
effects of four chlorobenzenes on serum sex steroids and hepatic microsome enzyme activities in crucian carp, carassius auratus.four chlorobenzenes (chlorobenzene, 1,3-dichlorobenzene, 1,4-dichlorobenzene, p-chloro-methylbenzene) were administrated to the crucian carps (carassius auratus) by peritoneal injections in the laboratory for 30 days. serum testosterone and 17 beta-estradiol concentrations were detected using radioimmunology assay (ria), and the activities of two hepatic microsome enzymes, glutathione s-transferase (gst) and udp-glucuronosyltransferase (udpgt), were measured using the modified methods as describ ...200415294436
identification of a novel c1q family member in color crucian carp (carassius auratus) ovary.potential roles of c1q/tumor necrosis factor (tnf) superfamily proteins have been observed in vertebrate oogenesis and oocyte maturation, but no ovary-specific member has been identified so far. in this study, we have cloned and identified a novel member of c1q family with a c1q domain in the c-terminal from fully grown oocyte cdna library of color crucian carp and demonstrated that the gene might be specifically expressed in ovary and therefore designated as carassius auratus ovary-specific c1q ...200415253877
cell culture of clonal ginbuna crucian carp hematopoietic cells: differentiation of cultured cells into erythrocytes in vivo.we cultivated kidney hematopoietic cells from clonal triploid ginbuna crucian carp, carassius auratus langsdorfii. proliferating cells from hematopoietic cell cultures were harvested and injected into tetraploid hybrids (clonal triploid ginbuna and goldfish hybrid) which possess three sets of chromosomes from a triploid clone and a haploid set of chromosomes from goldfish (carassius auratus). after injection of cultured triploid cells (donor cells), blood samples were obtained from tetraploid hy ...200415183027
development of an immunosensor for the detection of vitellogenin using impedance spectroscopy.a simple and direct immunosensor for the determination of carp (carassius auratus) vitellogenin (vtg), a female-specific protein, has been proposed based on an antibody-captured conducting polymer-coated electrode. the monoclonal antibody specific to carp (c. auratus) vtg was immobilized by covalent coupling to the carboxylic acid group on the polymer. the antibody immobilization and antibody-antigen interaction have been demonstrated by means of quartz crystal microbalance and impedance spectro ...200415046756
pluripotency of cryopreserved blastomeres of the goldfish.to examine the pluripotency of cryopreserved blastomeres, we transplanted them into blastula. donor blastomeres were prepared from blastula of goldfish (carassius auratus) and cryopreserved in liquid nitrogen for two months. fifty-five percent and 44% of blastomeres survived after thawing. cryopreserved blastomeres were transplanted to the blastula of triploid crucian carp (c. a. longsdorfii), which reproduces gynogenetically in nature. at four days after the operation, resultant chimeric embryo ...200414743512
coexistence of a sexual and an unisexual form stabilized by parasites.an unisexual species (either parthenogenetic or gynogenetic form) often coexists sympatrically with a closely related anisogamous sexual species, forming a unisexual complex. this is puzzling because, all else being equal, a sexual population with the two-fold cost of sex (or the cost of producing males) cannot coexist with a unisexual population. this suggests that some ecological processes are at work to make possible the coexistence of the unisexual complex. field and laboratory studies sugge ...200414643188
[triploid crucain carp-allotetraploid hybrids (male) x goldfish( hermaphrodite)].a new type of triploid crucian carp was generated by mating the males of the allotetraploid hybrids of red crucian carp (carassius auratus red var.) (female) x common carp (cyprinus carpio l.) (male), with the females the goldfish( carassius auratus red var.) which was red in color and possessed the biforked tails. the biological characteristics of one-year old triploid crucian carp including the number and karotype of chromosome, gonadosomatic indices and gonadal structures, appearance and grow ...200415468916
immunotoxicity of pentachlorophenol on macrophage immunity and igm secretion of the crucian carp (carassius auratus). 200415386086
effects of growth hormone on leucine absorption, intestinal morphology, and ultrastructure of the goldish intestine.the mechanisms whereby exogenous growth hormone modulates intestinal structure and function in fish were investigated. goldfish (carassius auratus) were fed commercial flake diet sprayed with recombinant carp growth hormone (cgh) daily for 1 month. control animals received food sprayed with the vehicle. after 1 month of daily feedings, body mass and length were determined, and animals were sacrificed to study intestinal characteristics. sections of foregut were removed after determination of tot ...200415644934
[karyotypic analysis from two clones of gynogenetic pengze crucian carp (carassius auratus of pengze)].pengze crucian carp (carassius auratus of pengze), one of the most popular cultural fishes in china, is an endemic bisexual population with natural gynogenetic reproduction mode. just like silver crucian carp, investigation on the karyotype of pengze crucian carp is important to understanding the diversity and the evolution of the unisexual vertebrates. two distinguishable gynogenetic clones of pengze crucian carp, named clone h and clone l, respectively,were detected recently. in this study, th ...200415481531
[use of brain weight in population studies of carp fry]. 2004499036
a separation-free amperometric immunosensor for vitellogenin based on screen-printed carbon arrays modified with a conductive polymer.a disposable amperometric immunosensor was studied for the rapid detection of carp (carassius auratus) vitellogenin (vtg). the sensor was fabricated based on screen-printed carbon arrays (spcas) containing eight carbon working and an integrated carbon counter electrodes. to construct the sensor, a conducting polymer (poly-terthiophene carboxylic acid) was electropolymerized on the surface of working electrodes and the polymer-coated spcas was characterized by sem. horseradish peroxidase (hrp) an ...200515681194
impacts of quicklime (cao) on the toxicity of copper (cuso4, 5h2o) to fish and fish food organisms.static bioassays of 96 h duration were conducted in the laboratory using fry of common carp (cyprinus carpio), adult tubificid worm (branchiura sowerbyi) and adult copepod plankton (cyclops viridis) to determine lc50 values of cu and cao to these organisms and effects of interaction between cu and cao. ninety-six hour lc(50) values of cu to fry of common carp, worm and copepod were found to be 1.40 mgl(-1), 0.08 mgl(-1) and 0.03 mgl(-1) respectively. cao up to 500 mgl(-1) did not produce any mor ...200516168741
evaluation of pesticides and metals in fish of the dniester river, moldova.the dniester river is one of the largest rivers in eastern europe and receives input from numerous anthropogenic activities. in october 2001, fish samples (abramis brama, rutilus rutilus heckeli, perca fluviatilis, chondrostoma nasus, carassius auratus gibelio, and cyprinus carpio) were collected and analyzed for metals and various pesticides. maximum total ddt pesticide concentrations were between 27.7 and 34.2 ng/g wet weight in muscles and gonads, respectively. dde isomers comprised up to 70% ...200515914239
an overview of health control in croatian aquaculture.aquaculture is an important segment of croatian fishing industry with long tradition of carp and rainbow trout farming as well as marine aquaculture represented by shellfish (flat oyster and mussels), sea bass and sea bream cultivation and atlantic bluefin tuna fattening. the fish and shellfish diseases survey is regulated by "decree on the measures of animal health protection against infectious and parasite diseases" issued yearly by ministry of agriculture. this report derives from systematic ...200516244940
female morphology and systematic status of philometroides cyprini (nematoda: philometridae), a parasite of carp.gravid females of philometroides were found under the scales of stock carp cyprinus carpio l. imported from lithuania to the czech republic in the spring of 1997 and kept in a quarantine pond. these specimens and those obtained from pond-cultured carp in russia (moscow district) were used for detailed morphological studies, including scanning electron microscopy. the results confirm that the valid name of this parasite is philometroides cyprini (ishii, 1931), whereas philometra lusii vismanis, 1 ...200516385815
development of expression vectors for transgenic fish.genetic alteration of fish is important for aquatic biotechnology as well as for investigating molecular interactions that occur during vertebrate development. the numerous, large, transparent, and externally fertilized eggs of many fish species make them ideally suitable for genetic manipulation, especially for production of transgenic animals. genetic engineering of fish requires suitable expression vectors. accordingly, we developed two fish expression vectors, fv-1 and fv-2, which contain th ...20051366961
[morphofunctional types of synapses in the neurosecretory cells of the carp preoptic nucleus].the ultrastructure of synaptic endings of the neurosecretory cells of the nucleus preopticus was examined in adult cyprinus carpio l. two of synpatic endings occur: type i--small agranular vesicles and large granular vesicles, type ii--only agranular vesicles. the functioning of the nucleus preopticus neurosecretory cells in cyprinus carpio l is presumably controlled by the synpatic endings of the adrenergic (synaptic endings of type i) as well as of the cholinergic (synaptic endings of type ii) ...20051231093
studies on the nutrition of marine flatfish. the thiamin requirement of turbot (scophthalmus maximus).1. seven groups of young turbot (scophthalmus maximus) were given diets containing graded levels of thiamin (0-19--50 mg/kg) for 16 weeks and their growth rate was measured during this period. 2. good growth was obtained on all these treatments except in the group given the lowest dietary thiamin level (0-19 mg/kg). these fish grew normally until the 12th week but thereafter their weight did not increase. 3. measurements of erythrocyte transketolase (sedoheptulose-7-phosphate: d-glyceraldehyde-3 ...20051201262
mechanical responses to linearly rising currents in the red and white muscles of the silver carp.the mechanical response of the red and white muscles of the silver carp was investigated using linearly stimulating currents. in the presence of tetrodotoxin, the peak tension increased when the rate of rise was reduced to about 0.3 ma/sec in the red muscle, and to about 3 ma/sec in the white muscle, provided that the maximum current applied always reached 3 ma. further reduction of the rate of rise decreased the tension, particularly in the white muscle. low concentrations of caffeine (0.5 mm) ...20051142568
differential spontaneous killing of human and murine tumour cell lines by leucocyte subpopulations from carp peripheral blood leucocytes.cell populations from carp (cyprinus carpio l.) peripheral blood leucocytes (pbls) were examined for nonspecific cytotoxicities. by using monoclonal antibodies (mabs) against carp thrombocytes (tcl-hb8) and both neutrophils and monocytes (tcl-be8), pbls with a density of 1.08 g ml-1 were separated into three fractions: thrombocytes, a mixture of neutrophils and monocytes, and other cells (mainly lymphocytes), and the separated cells were tested for cytotoxic activities against mammalian tumour c ...200515752650
sandwich immunoassay for determination of vitellogenin in golden grey mullet (liza aurata) serum as a field exposure biomarker.vitellogenin (vtg) is a protein produced in the liver of oviparous animals in response to oestrogens. abnormal production of vtg by males, therefore, is used as a biological indicator of exposure to xeno-oestrogens. in this study, a sandwich-elisa for measuring vtg in liza aurata (golden grey mullet) was developed and validated. plasma vtg was purified from 17beta-oestradiol-injected immature individuals of mullet, by size-exclusion and ion-exchange chromatography. polyclonal antibodies against ...200515756602
[phylogeny of chinese catfishes inferred from mitochondrial cytochrome b sequences].the mitochondrial dna cytochrome b gene was sequenced from 27 catfish species representing 11 families and 24 genera catfishes in china. aligning with cytochrome b sequences of eight catfish species from north america and africa retrieved from genbank, and selecting astyanax mexicanu, cyprinus carpio, and sardinops melanostictus as outgroups, we constructed a matrix of 38 dna sequences. the phylogenetic trees were constructed by using bayesian method and maximum parsimony (mp) method. the result ...200515759861
cloning of the koi herpesvirus (khv) gene encoding thymidine kinase and its use for a highly sensitive pcr based diagnosis.outbreaks with mass mortality among common carp cyprinus carpio carpio and koi cyprinus carpio koi have occurred worldwide since 1998. the herpes-like virus isolated from diseased fish is different from herpesvirus cyprini and channel catfish virus and was accordingly designated koi herpesvirus (khv). diagnosis of khv infection based on viral isolation and current pcr assays has a limited sensitivity and therefore new tools for the diagnosis of khv infections are necessary.200515774009
recently published papers: from superbugs to superbowl.several papers are discussed including the carp (coronary artery revascularisation prophylaxis) trial, examining the role, if any, of preoperative coronary revascularisation and a study examining the heart rate in the intensive care unit (icu) population. we also take a look at infections in the icu with particular regard to a recent study which examined isolating patients with methicillin-resistant staphylococcus aureus (mrsa).200515774060
the complete genomic sequence of the carp fast skeletal myosin heavy chain gene.we have determined the complete dna nucleotide sequence of the carp cyprinus carpio fast skeletal myosin heavy chain (myh) gene. introns and exons were predicted by comparison with the corresponding carp myh cdnas previously reported. the gene encoded the entire mrna transcript and contained 5958 nucleotides (nt) including 77 nt 5'-untranslated region, 5796 nt coding region for 1931 amino acids, and 85 nt 3'-untranslated region. the coding region was split by 38 introns and the complete gene con ...200515777658
physiological function and metabolism of free d-alanine in aquatic animals.aquatic crustaceans and some bivalve mollusks contain a large amount of free d-alanine (up to 100 mumol/g wet wt.) in their tissues. under high salinity stress, crustaceans and bivalve mollusks largely accumulate d- and l-alanine irrespective of species examined, together with l-glutamine, l-proline, and glycine of which increases are species dependent. these data indicate that d-alanine is one of the major compatible osmolytes responsible for the intracellular isosmotic regulation in the tissue ...200516141518
interaction between the blood fluke, sanguinicola inermis and humoral components of the immune response of carp, cyprinus carpio.the effect of sanguinicola inermis on serum antibody and complement activity in cyprinus carpio was assessed using an elisa and haemolytic assays. possible immune evasion strategies were assessed using immunodetection of host proteins on the surface of the parasite. carp acclimatized to 20 or 25 degrees c were infected by exposure to 500 cercariae or injected intraperitoneally with 150 cercariae, and serum monitored over a 63-day period. in cercariae-injected carp, irrespective of time and tempe ...200516145943
parasite assemblages of crucian carp (carassius carassius)--is depauperate composition explained by lack of parasite exchange, extreme environmental conditions or host unsuitability?recent studies on parasite communities have considered the factors which contribute to variability in parasite infections among populations of one host species. this paper examined parasite assemblages of crucian carp (carassius carassius) in a natural set-up of two distinctive environments inhabited by the same host species and differing in respect to composition of other fish species: ponds, where extreme conditions prevent other fish species from occurring and lakes, where crucian carp coexis ...200516145944
exceeding tertiary standards with a pond/reed bed system in norway.at vidaråsen in norway sewage from a community consisting of 160 people, including a dairy, a food processing workshop, a bakery and a laundry is treated using a pond/reed bed system. the system consists of sludge settlement, pre-treatment surface/vertical-flow constructed wetlands, a 5 m deep enhanced facultative pond, three stabilization ponds, a planted sand filter and finally two horizontal-flow constructed wetlands filled with lightweight aggregate (filtralite-p). the enhanced facultative p ...200516042271
identification of keratins and analysis of their expression in carp and goldfish: comparison with the zebrafish and trout keratin catalog.with more than 50 genes in human, keratins make up a large gene family, but the evolutionary pressure leading to their diversity remains largely unclear. nevertheless, this diversity offers a means to examine the evolutionary relationships among organisms that express keratins. here, we report the analysis of keratins expressed in two cyprinid fishes, goldfish and carp, by two-dimensional polyacrylamide gel electrophoresis, complementary keratin blot binding assay, and immunoblotting. we further ...200516059702
homology model and molecular dynamics simulation of carp ovum cystatin.in this study, a homology model of carp ovum cystatin was constructed based on the crystal structure of chicken egg white cystatin. the results of amino acid sequence alignment indicate that these two proteins exhibit 36.11% of sequence identity. the resultant homology model reveals that carp ovum cystatin shares similar folds as chicken egg white cystatin, particularly in the conserved regions of q48-v49-g52 and p98-w99 and the locations of two disulfide bonds, c67-c76 and c90-c110. however, th ...200516080717
arachidonic acid and long-chain n-3 polyunsaturated fatty acid contents in meat of selected poultry and fish species in relation to dietary fat sources.arachidonic acid (aa) content, long-chain n-3 polyunsaturated fatty acid (pufa) equivalent [lce; calculated as 0.15 x linolenic acid (la) + eicosapentaenoic acid (epa) + docosahexaenoic acid (dha)], and pufa n-6/pufa n-3 ratio were determined in meat [breast meat (bm), thigh meat (tm), and fillets (f), respectively] within four sets of chickens, five sets of turkeys, one set of common carp, and four sets of rainbow trout, fed either commercial diet or diets with manipulated pufa n-3 and pufa n-6 ...200516104803
[effect of increasing the omega-3 fatty acid in the diets of animals on the animal products consumed by humans].as shown by huge amount of assays in human as well as in animal models, w-3 polyunsaturated fatty acids play important role in the development and maintenance of different organs, primarily the brain, and could be useful in the prevention of different pathologies, mainly the cardiovascular diseases, and, as proposed recently, some psychiatric, dermatological or rheumatological disorders. for ala, the major and cheapest source for human is rapeseed oil (canola oil), and walnut "noix de grenoble" ...200516115466
histopathological and electron microscopy studies on sleepy disease of koi cyprinus carpio koi in japan.koi sleepy disease (ksd) usually occurs when koi cyprinus carpio koi are taken from nursing earthen ponds and placed in cement-lined ponds containing fresh water in spring and autumn in japan. we transfered koi from an earthen pond to tanks containing fresh water and 0.5 % salt water in an attempt to replicate ksd and prevent the onset of ksd, respectively, in the laboratory. ksd broke out after 4 to 5 d, followed by mass mortality (76%: 95/125 fish) within 17 d, in the fresh water. diseased fis ...200516119888
detection and sequence analysis of dna polymerase and major envelope protein genes in koi herpesviruses derived from cyprinus carpio in gunma prefecture, japan.koi herpesvirus (khv), which is believed to be an emerging virus, causes fatal diseases in carps. since the 1990s, the presence of khv has been confirmed in several countries. in japan, from 2003 to 2004, large outbreaks of khv infection in farmed carps resulted in the death of numerous fishes. from april to may 2004, we collected 43 dead or dying carps exhibiting typical symptoms of khv infection in gunma prefecture. to conduct a molecular epidemiologic study of khv in our prefecture, we amplif ...200516125879
polar filament discharge of myxobolus cerebralis actinospores is triggered by combined non-specific mechanical and chemical cues.this study presents initial evidence for the requirement of both chemical and mechanical stimuli to discharge polar capsules of myxobolus cerebralis actinospores, the causative agent of salmonid whirling disease. the obligate need for combined discharge triggers was concluded from data obtained in a before/after experimental set-up carried out with individual locally immobilized actinospores. homogenized rainbow trout mucus as chemostimulus and tangency of the apical region of the spores to achi ...200516255819
the molecular regulation of exercised-induced muscle fibre hypertrophy in the common carp: expression of myod, pcna and components of the calcineurin-signalling pathway.growth was investigated over 16 d in juvenile common carp (cyprinus carpio l.) held in either static water (tank rested, tr16) or exercised in a flume at 2.5-3.2 body lengths s-1 for 18 h a day (exercised, e16). relative to the start of the experiment (tr0), the tr16 group showed a 31% increase in body mass (specific growth rate, 1.57% d-1), whereas there was no net change in the e16 group. there was, however, a significant exercise-induced hypertrophy of slow muscle fibres with average fibre cr ...200516185906
activity of superoxide dismutase (sod) and concentration of thiobarbituric acid reactive substances (tbars) in liver and muscles of some fish.in this study we examined superoxide dismutase (sod) activity and thiobarbituric acid reactive substances (tbars) concentration in liver and muscles of four fish species: the carp, the brown trout, the white cod and the flounder. higher sod activity and higher tbars concentration was revealed in the tissues of marine fish in comparison to freshwater fish. the highest sod activity was observed in the cod while the highest tbars concentration was in the flounder. the observed differences are proba ...200516196213
hey basic helix-loop-helix transcription factors are repressors of gata4 and gata6 and restrict expression of the gata target gene anf in fetal hearts.the hey basic helix-loop-helix transcription factors are downstream effectors of notch signaling in the cardiovascular system. mice lacking hey2 develop cardiac hypertrophy, often associated with congenital heart defects, whereas combined hey1/hey2 deficiency leads to severe vascular defects and embryonic lethality around embryonic day e9.5. the molecular basis of these disorders is poorly understood, however, since target genes of hey transcription factors in the affected tissues remain elusive ...200516199874
modulating effects of humic acids on genotoxicity induced by water disinfectants in cyprinus carpio.the use of chlorinated disinfectants during drinking-water production has been shown to generate halogenated compounds as a result of interactions of humic acids with chlorine. such chlorinated by-products have been shown to induce genotoxic effects and consumption of chlorinated drinking-water has been correlated with increased risk for cancer induction in human populations. the aim of this work was to test the potential genotoxic effects on circulating erythrocytes of the fish cyprinus carpio ...200516202643
an inexpensive and rapid diagnostic method of koi herpesvirus (khv) infection by loop-mediated isothermal amplification.koi herpesvirus (khv) affects both juvenile and adult common carp and koi, and is especially lethal to fry. the high mortalities caused by the disease have had a negative impact on the international koi trade. different diagnostic techniques have been used to detect khv, including: isolation of the virus in cell culture, electron microscopy, several pcr tests, elisa and in situ hybridisation. all of these methods are time consuming, laborious and require specialised equipment.200516216123
efficient expression of transgenes in adult zebrafish by electroporation.expression of transgenes in muscle by injection of naked dna is widely practiced. application of electrical pulses at the site of injection was demonstrated to improve transgene expression in muscle tissue. zebrafish is a precious model to investigate developmental biology in vertebrates. in this study we investigated the effect of electroporation on expression of transgenes in 3-6 month old adult zebrafish.200516221312
environmental contaminants and biomarker responses in fish from the rio grande and its u.s. tributaries: spatial and temporal trends.we collected, examined, and analyzed 368 fish of seven species from 10 sites on rivers of the rio grande basin (rgb) during late 1997 and early 1998 to document temporal and geographic trends in the concentrations of accumulative contaminants and to assess contaminant effects on the fish. sites were located on the mainstem of the rio grande and on the arroyo colorado and pecos river in texas (tx), new mexico (nm), and colorado. common carp (cyprinus carpio) and largemouth bass (micropterus salmo ...200516227080
role of microfilaments and microtubules in the invasion of epc cells by aeromonas hydrophila.the influence of the cytoskeleton on the invasion of aeromonas hydrophila strain ahj-1, isolated from diseased fish, in the monolayer cell of epithelioma papillosum cells of carp (epc) was evaluated by the recovery of gentamicin-resistant bacteria from triton x-100 cell lysates. the depolymerization of microfilaments (mf) by cytochalasin b and d inhibited the uptake of a. hydrophila in a dose-dependent manner and that of microtubules (mt) by colchicines and nocodazole did not affect the invasion ...200516000113
[deterministic analysis as a tool to investigate the contingency of various components of biocenosis].specific application of deterministic analysis to investigate the contingencies of various components of natural biocenosis was illustrated by the example of fish production and biomass of phyto- and zooplankton. deterministic analysis confirms the theoretic assumptions on food preferences of herbivorous fish: both silver and bighead carps avoided feeding on cyanobacteria. being a facultative phytoplankton feeder, silver carp preferred microalgae to zooplankton. deterministic analysis allowed us ...200516004266
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