Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [comparative study of some technics for determination of transferrin in the blood]. | 2016 | 4976288 | |
| no excuse for rh failure. | 2016 | 4981721 | |
| durability of prosthetic heart valves. | 2016 | 4926359 | |
| new problems of myelomeningocele. | 2016 | 4869196 | |
| [gonioscopic findings in aphakia. ii]. | 2016 | 4865122 | |
| isolation and characterization of a vinylic tricarboxyl porphyrin from bile. | 2016 | 4836427 | |
| pyriformsinusosotomy. a useful technique for temporary or permanent tube feeding. | 2016 | 4959520 | |
| [10 years' studying of plant viruses at the institute of microbiology and virology of the ukrainian academy of sciences]. | 2016 | 4899491 | |
| platelet survival in patients with rheumatic heart disease. | 2016 | 4811098 | |
| [clinical comparison of various types of starr-edwards prosthesis based on pulmonary circulation and left ventricular functions]. | 2016 | 4856990 | |
| [case of syndrome of the medial longitudinal fasciculus following head injury]. | 2016 | 4796518 | |
| axon outgrowth enhanced by a previous nerve injury. | 2016 | 4711805 | |
| blood distribution through the lung after pneumonectomy: a quantitative assessment by 131-i. | 2016 | 5091637 | |
| hypersomatotropism and acanthosis nigricans in two brothers. | 2016 | 4843205 | |
| mary--a paranoid schizophrenic. | 2016 | 4844741 | |
| [drug therapy in hypertension]. | 2016 | 4766384 | |
| [reaction of tricarbethoxymethane. v. amidoesters]. | 2016 | 5093140 | |
| idiopathic thrombocytopenic purpura. an unusual pregnancy complication. | 2016 | 5061743 | |
| [facilitation of labor]. | 2016 | 5047589 | |
| [changes in plasma lipids in normal subjects in relation to age and sex. 3. total lipids, triglycerides, phospholipids, alpha- and beta-lipoproteic cholesterol and single nefa (c16-c18) in pre-senile subjects]. | 2016 | 5030359 | |
| rejection of the generic name dendrocystis rees, 1962, and its replacement by arborocystis nov.nom. protozoa: gregarinomorpha. | 2016 | 4960245 | |
| [scanning of the distal perfusion pressure in skin and muscle with 133 xenon in estimation of the pressure situation in pregangrene--gangrene]. | 2016 | 4913102 | |
| selective antagonism of central nervous system depressants by dh-524; a comparative study with bemegride. | 2016 | 4771574 | |
| [aging of the patients with old cranial injuries. (catamnestic study of 88 patients hospitalized in psychiatric environment)]. | 2016 | 4725280 | |
| control of anthracnose disease of figs by fungicides and antifungal antibiotic aureofungin. | 2016 | 4763643 | |
| [biochemical effects of continuous and discontinuous muscular work (interval work)]. | 2016 | 4720306 | |
| [biochemical effects of continuous and discontinuous muscular work (interval work)]. | 2016 | 4720306 | |
| [letter: influence of meteorological conditions on the occurrence of spontaneous pneumothorax]. | 2016 | 4849084 | |
| [diagnosis and therapy of chronic septic diseases]. | 2016 | 5034424 | |
| degradation products and the role of coagulation in "persistent" glomerulonephritis. | 2016 | 4931741 | |
| coloboma of the iris and choroid and retinal detachment in oculo-auricular dysplasia (goldenhar syndrome). | 2016 | 5076735 | |
| planning for briggs. 1. | 2016 | 4844745 | |
| nondialyzable urinary hydroxyproline as an index of bone collagen formation. | 2016 | 4983851 | |
| biogeochemical and hydrological drivers of the dynamics of vibrio species in two patagonian estuaries. | the ecology of the most relevant vibrio species for human health and their relation to water quality and biogeochemistry were studied in two estuaries in argentinian patagonia. vibrio cholerae and vibrio parahaemolyticus were reported in >29% of cases at the río colorado and río negro estuaries. neither the pandemic serogroups of vibrio cholerae o1, vibrio cholerae o139 nor the cholera toxin gene were detected in this study. however, several strains of v. cholerae (not o1 or o139) are able to ca ... | 2017 | 27871750 |
| non-cholera vibrios: the microbial barometer of climate change. | there is a growing interest in the role of climate change in driving the spread of waterborne infectious diseases, such as those caused by bacterial pathogens. one particular group of pathogenic bacteria - vibrios - are a globally important cause of diseases in humans and aquatic animals. these gram-negative bacteria, including the species vibrio vulnificus, vibrio parahaemolyticus and vibrio cholerae, grow in warm, low-salinity waters, and their abundance in the natural environment mirrors ambi ... | 2017 | 27843109 |
| responses of mytilus galloprovincialis hemocytes to environmental strains of vibrio parahaemolyticus, vibrio alginolyticus, vibrio vulnificus. | marine bivalves are exposed to different types of bacteria in the surrounding waters, in particular of the vibrio genus. in the hemocytes of the mussel mytilus spp. immune responses to different vibrios have been largely characterized. however, little information is available on the hemocyte responses to human pathogenic vibrios commonly detected in coastal waters and bivalve tissues that are involved in seafood-borne diseases. | 2017 | 28390964 |
| season-specific occurrence of potentially pathogenic vibrio spp. on the southern coast of south korea. | vibrio species are widely distributed in warm estuarine and coastal environments, and they can infect humans through the consumption of raw and mishandled contaminated seafood. in this study, we aimed to isolate and observe the distribution of enteropathogenic vibrio spp. from environments of the southern coast of south korea over a season cycle. a total of 10,983 isolates of vibrio spp. were obtained from tidal water and mud samples over a 1-year period from five sampling sites along the southw ... | 2017 | 27836844 |
| mechanisms for pseudoalteromonas piscicida-induced killing of vibrios and other bacterial pathogens. | pseudoalteromonas piscicida are gram-negative gammaproteobacteria found in the marine environment. three strains of pigmented p. piscicida were isolated from seawater and partially characterized by inhibition studies, electron microscopy, and proteolytic enzyme production. growth inhibition and death occurred around colonies of p. piscicida on lawns of the naturally-occurring marine pathogens vibrio vulnificus, v. parahaemolyticus, v. cholerae, photobacterium damselae and shewanella algae inhibi ... | 2017 | 28363962 |
| the membrane localization domains of two distinct bacterial toxins form a 4-helix-bundle in solution. | membrane localization domain (mld) was first proposed for a 4-helix-bundle motif in the crystal structure of the c1 domain of pasteurella multocida toxin (pmt). this structure motif is also found in the crystal structures of several clostridial glycosylating toxins (tcda, tcdb, tcsl, and tcna). the ras/rap1-specific endopeptidase (rrsp) module of the multifunctional autoprocessing repeats-in-toxins (martx) toxin produced by vibrio vulnificus has sequence homology to the c1-c2 domains of pmt, inc ... | 2017 | 27977897 |
| biotreatment of petrochemical wastewater: a case study from northern tunisia. | a full-scale study has been conducted to assess the bioaugmentation efficiency of trickling filter process to treat petrochemical wastewater from a lubricant industry recycling waste oils. during 45 weeks, the organic loading rate (olr) in the trickling filter was increased stepwise from 0.9 to 4 kg of chemical oxygen demand (cod)/(m3·day) at the end of the upgrading period as the flow rate (fr) reached the value of 30 m3/day. the removal, obtained in terms of percentage, for cod ranged from 60 ... | 2017 | 27094680 |
| two new benzoate derivatives and one new phenylacetate derivative from a marine-derived fungus engyodontium album. | two new benzoate derivatives, ethyl 3,5-dimethoxy-2-propionylbenzoate (1) and ethyl 3,5-dihydroxy-2-propionylbenzoate (2), and one new phenylacetate derivative, ethyl 3,5-dimethoxy-2-propionylphenylacetate (3), together with 9 known compounds, were isolated from the fermentation of engyodontium album derived from deep sea sediment. their structures were elucidated by spectroscopic techniques, such as nmr, ir and hresims. compound 3 exhibited inhibitory activities against methicillin-resistant st ... | 2017 | 27750436 |
| a tfpi-1 peptide that induces degradation of bacterial nucleic acids, and inhibits bacterial and viral infection in half-smooth tongue sole, cynoglossus semilaevis. | tissue factor pathway inhibitor 1 (tfpi-1) is a serine protease inhibitor that inhibits tissue factor (tf)-mediated coagulation. the c-terminal region of tfpi-1 could be cleaved off and proved to be antimicrobial against a broad-spectrum of microorganism. in a previous study, a c-terminal peptide, tc24 (with 24 amino acids), derived from tongue sole (cynoglossus semilaevis) tfpi-1, was synthesized and found antibacterial against micrococcus luteus. in the present study, the antibacterial spectru ... | 2017 | 27840169 |
| tc38, a teleost tfpi-2 peptide that kills bacteria via penetration of the cell membrane and interaction with nucleic acids. | tissue factor pathway inhibitor 2 (tfpi-2) is an analog of tfpi-1 and a potent endogenous inhibitor of tissue factor (tf)-mediated blood coagulation. recent reports have proven that the c-terminal of tfpi-2 peptides in humans and several other vertebrates possesses antibacterial activity against gram-positive and gram-negative bacteria. in our previous study, we reported that the tfpi-2 peptide, tc38 in tongue sole (cynoglossus semilaevis) was active against micrococcus luteus. in this study, we ... | 2017 | 28263880 |
| edwardsiella tarda bacteremia. a rare but fatal water- and foodborne infection: review of the literature and clinical cases from a single centre. | edwardsiella tarda bacteremia (etb) can be a fatal disease in humans. | 2017 | 26744588 |
| expression, purification and enzymatic characterization of undecaprenyl pyrophosphate phosphatase from vibrio vulnificus. | undecaprenyl pyrophosphate phosphatase (uppp), a cell membrane integral enzyme, catalyzes the dephosphorylation of undecaprenyl pyrophosphate to undecaprenyl phosphate, which is an essential carrier lipid in bacterial cell wall synthesis. we previously purified e. coli uppp and concluded that its catalytic site is likely located in the periplasm. to search for additional natural uppp homologs to elucidate what constitutes a common catalytic mechanism and to gain a better chance of obtaining high ... | 2017 | 28302513 |
| plesiomonas shigelloides periprosthetic knee infection after consumption of raw oysters. | periprosthetic infections are a leading cause of morbidity after total joint arthroplasty. common pathogens include staphylococcus aureus, streptococcus, enterococcus, escherichia coli, and pseudomonas aeruginosa. however, there are many cases in which rare bacteria are isolated. this case report describes a periprosthetic knee infection caused by plesiomonas shigelloides. in the united states, p shigelloides and 2 other vibrionaceae family members, vibrio vulnificus and vibrio parahaemolyticus, ... | 2017 | 28235124 |
| development of a regulatable expression system for the functional study of vibrio vulnificus essential genes. | we developed a regulatable gene expression system for vibrio vulnificus, which contains a laciq-ptrc cassette. monomeric red fluorescence protein (mrfp) was used as a reporter to test this system. the results showed that this system tightly controlled the expression of mrfp without leaky expression and was suitable for the controlled expression of genes encoding recombinant proteins in v. vulnificus. to demonstrate the utility of this system, a dominant negative form of v. vulnificus vvmo6_rs049 ... | 2017 | 28044234 |
| mechanism of anti-vibrio activity of marine probiotic strain bacillus pumilus h2, and characterization of the active substance. | vibriosis is a major epizootic disease that impacts free-living and farmed fish species worldwide. use of probiotics is a promising approach for prevention of vibrio infections in aquaculture. a probiotic anti-vibrio strain, bacillus pumilus h2, was characterized, and the mechanism of its effect was investigated. all 29 vibrio strains tested were growth-inhibited by h2. the anti-vibrio substance present in cell-free supernatant of h2 was purified and characterized by reversed-phase hplc. minimum ... | 2017 | 28097594 |
| pathogenic mechanisms of actin cross-linking toxins: peeling away the layers. | actin cross-linking toxins are produced by gram-negative bacteria from vibrio and aeromonas genera. the toxins were named actin cross-linking domains (acd), since the first and most of the subsequently discovered acds were found as effector domains in larger martx and vgrg toxins. among recognized human pathogens, acd is produced by vibrio cholerae, vibrio vulnificus, and aeromonas hydrophila. upon delivery to the cytoplasm of a host cell, acd covalently cross-links actin monomers into non-polym ... | 2017 | 27858184 |
| o-heterocyclic derivatives with antibacterial properties from marine bacterium bacillus subtilis associated with seaweed, sargassum myriocystum. | the brown seaweed, sargassum myriocystum associated with heterotrophic bacterium, bacillus subtilis mtcc 10407 (jf834075) exhibited broad-spectra of potent antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio vulnificus, and vibrio parahaemolyticus. b. subtilis mtcc 10407 was found to be positive for polyketide synthetase (pks) gene, and therefore, was considered to characterize secondary metabolites bearing polyketide backbone. using bioassay-guided fractionation, t ... | 2017 | 27624095 |
| cmc stabilized nano silver synthesis, characterization and its antibacterial and synergistic effect with broad spectrum antibiotics. | in the present study silver nanoparticles were synthesized by reduction of agno3 using aqueous cmc solution, which acts as both reducing and capping agent. the formation of agno3 nanoparticles was observed visually by color change and these nanoparticles were characterized through uv-vis spectroscopy, ftir, xrd, sem, eds and afm. the ftir peaks observed to be ranging from 3300 to 605cm(-1). the afm image clearly showed the surface morphology of well dispersed nanoparticles. sem image illustrates ... | 2017 | 28024537 |
| raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets. | microbiological quality of 206 raw ready-to-eat seafood samples was investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster and squid) and distribution channels (fishery, hyper and online market). enumeration of aerobic plate count and total coliforms (tc) and pathogenic bacteria (bacillus cereus, staphylococcus aureus and vibrio parahaemolyticus) was performed, and level of microbiological quality was classified into four groups: satisfactory, acceptable, unsatisfactory ... | 2017 | 27747902 |
| emerging causes of superficial and invasive infections following marine injuries and exposures. | soft tissue bacterial infections following aquatic animal bites, stings, and minor injuries occur commonly and usually on the extremities in fishermen and beachgoers worldwide after freshwater and saltwater exposures. louisiana has more tidal, saltwater, and brackish water shorelines (more than 7,000 miles) than any other state, including alaska and hawaii. as a result, louisiana residents are often exposed to marine pathogens when fishing or working offshore or when enjoying louisiana's miles o ... | 2017 | 25075726 |
| a rare glimpse into the morbid world of necrotising fasciitis: flesh-eating bacteria vibrio vulnificus. | necrotising fasciitis is one of the fatal skin and soft tissue infections. vibrio vulnificus is a rare cause of necrotising fasciitis; however, the disease is one of the major manifestations of the bacteria. here, we report one such case in a middle-aged male patient. he presented with the signs of bilateral lower limb cellulitis and altered sensorium. v. vulnificus was isolated from blood culture and also from debrided tissue. though the organism is well characterised, it is a rare causative ag ... | 2017 | 27514968 |
| differences in abundances of total vibrio spp., v. vulnificus, and v. parahaemolyticus in clams and oysters in north carolina. | filter feeding shellfish can concentrate pathogenic bacteria, including vibrio vulnificus and vibrio parahaemolyticus, as much as 100-fold from the overlying water. these shellfish, especially clams and oysters, are often consumed raw, providing a route of entry for concentrated doses of pathogenic bacteria into the human body. the numbers of foodborne infections with these microbes are increasing, and a better understanding of the conditions that might trigger elevated concentrations of these b ... | 2017 | 27793822 |
| high salinity relaying to reduce vibrio parahaemolyticus and vibrio vulnificus in chesapeake bay oysters (crassostrea virginica). | cases of vibrio infections in the united states have tripled from 1996 to 2009 and these infections are most often associated with the consumption of seafood, particularly oysters (crassostrea virginica). information is needed on how to reduce numbers of vibrio parahaemolyticus and vibrio vulnificus in bi-valve molluscan shellfish (for example, oysters). the purpose of this study was to evaluate the effectiveness of high salinity relaying or treatment in recirculating aquaculture systems (rass) ... | 2017 | 28099766 |
| occurrence and virulence properties of vibrio and salinivibrio isolates from tropical lagoons of the southern caribbean sea. | the vibrionaceae are gram-negative bacteria present in marine and estuarine environments worldwide, including several species known as important pathogens to humans and aquatic organisms. the aim of this research was to investigate the occurrence and virulence properties of vibrio and salinivibrio isolated from lagoons at cuare wildlife refuge and margarita island in the southern caribbean sea. water, plankton and oyster samples were collected during october 2011 and march 2012 and examined by s ... | 2017 | 28324231 |
| clinical features and treatment of patients with vibrio vulnificus infection. | infections with vibrio vulnificus are commonly fatal, and the speed and accuracy of diagnosis and treatment is directly linked to mortality. the main aims of this study were to investigate the clinical characteristics of six patients with v. vulnificus infections retrospectively and to determine the effect of treatment with tigecycline (tgc) alone compared with doxycycline plus ceftazidime (dox/caz). | 2017 | 28347850 |
| occurrence of clinical genotype vibrio vulnificus in clam samples in mangalore, southwest coast of india. | vibrio vulnificus is an opportunistic human pathogen causing gastroenteritis, wound infection and primary septicemia. v. vulnificus population has been divided into subpopulations based on their phenotype and genotype characteristics. in this study, 38.5% (10/26) of clam (meretrix meretrix) samples obtained from mangalore markets were seen to harbor v. vulnificus. biochemical characterization of v. vulnificus isolates showed the strains to belong to biotype 1 phenotype. genotyping of strains usi ... | 2017 | 28298693 |
| incidences of waterborne and foodborne diseases after meteorologic disasters in south korea. | climate change could increase the number of regions affected by meteorologic disasters. meteorologic disasters can increase the risk of infectious disease outbreaks, including waterborne and foodborne diseases. although many outbreaks of waterborne diseases after single disasters have been analyzed, there have not been sufficient studies reporting comprehensive analyses of cases occurring during long-term surveillance after multiple disasters, which could provide evidence of whether meteorologic ... | 2017 | 28283139 |
| the hydrogen peroxide hypersensitivity of oxyr2 in vibrio vulnificus depends on conformational constraints. | most gram-negative bacteria respond to excessive levels of h2o2 using the peroxide-sensing transcriptional regulator oxyr, which can induce the expression of antioxidant genes to restore normality. vibrio vulnificus has two distinct oxyrs (oxyr1 and oxyr2), which are sensitive to different levels of h2o2 and induce expression of two different peroxidases, prx1 and prx2. while oxyr1 has both high sequence similarity and comparable h2o2 sensitivity to other oxyr proteins, oxyr2 exhibits limited se ... | 2017 | 28264933 |
| two-step enhanced cancer immunotherapy with engineered salmonella typhimurium secreting heterologous flagellin. | we report a method of cancer immunotherapy using an attenuated salmonella typhimurium strain engineered to secrete vibrio vulnificus flagellin b (flab) in tumor tissues. engineered flab-secreting bacteria effectively suppressed tumor growth and metastasis in mouse models and prolonged survival. by using toll-like receptor 5 (tlr5)-negative colon cancer cell lines, we provided evidence that the flab-mediated tumor suppression upon bacterial colonization is associated with tlr5-mediated host react ... | 2017 | 28179508 |
| iutb participates in the ferric-vulnibactin utilization system in vibrio vulnificus m2799. | vibrio vulnificus, an opportunistic pathogen that causes a serious, often fatal, infection in humans, requires iron for its growth. this bacterium utilizes iron from the environment via the vulnibactin-mediated iron uptake system. the mechanisms of vulnibactin biosynthesis, vulnibactin export, and ferric-vulnibactin uptake systems have been reported, whereas the ferric-vulnibactin reduction mechanism in the cell remains unclear. the results of our previous study showed that vuub, a member of the ... | 2017 | 28150143 |
| regulation systems of protease and hemolysin production in vibrio vulnificus. | vibrio vulnificus, a gram-negative halophilic estuarine bacterium, is an opportunistic human pathogen that causes rapidly progressive fatal septicemia and necrotizing wound infection. this species also causes hemorrhagic septicemia called vibriosis in cultured eels. it has been proposed that a range of virulence factors play roles in pathogenesis during human and/or eel infection. among these factors, a metalloprotease (v. vulnificus protease [vvp]) and a cytolytic toxin (v. vulnificus hemolysin ... | 2017 | 28111826 |
| transcriptome analysis of the effect of polyunsaturated fatty acids against vibrio vulnificus infection in oreochromis niloticus. | vibrio vulnificus infection causes severe economic losses in oreochromis niloticus aquaculture by inducing pro-inflammatory cytokines, that lead to inflammation and mortality. omega-3 fatty acids, such as docosahexaenoic acid (dha) and eicosapentaenoic acid (epa), have been reported for their anti-inflammatory and antibacterial abilities in murine and zebrafish models. however, the anti-inflammatory and antibacterial functions of dha and epa in commercial aquaculture organisms such as oreochromi ... | 2017 | 28108339 |
| study of the antimicrobial activity of tilapia piscidin 3 (tp3) and tp4 and their effects on immune functions in hybrid tilapia (oreochromis spp.). | to address the growing concern over antibiotic-resistant microbial infections in aquatic animals, we tested several promising alternative agents that have emerged as new drug candidates. specifically, the tilapia piscidins are a group of peptides that possess antimicrobial, wound-healing, and antitumor functions. in this study, we focused on tilapia piscidin 3 (tp3) and tp4, which are peptides derived from oreochromis niloticus, and investigated their inhibition of acute bacterial infections by ... | 2017 | 28085905 |
| the effector domain region of the vibrio vulnificus martx toxin confers biphasic epithelial barrier disruption and is essential for systemic spread from the intestine. | vibrio vulnificus causes highly lethal bacterial infections in which the multifunctional autoprocessing repeats-in-toxins (martx) toxin product of the rtxa1 gene is a key virulence factor. martx toxins are secreted proteins up to 5208 amino acids in size. conserved martx n- and c-terminal repeat regions work in concert to form pores in eukaryotic cell membranes, through which the toxin's central region of modular effector domains is translocated. upon inositol hexakisphosphate-induced activation ... | 2017 | 28060924 |
| vibrio vulnificus: from oyster colonist to human pathogen. | 2017 | 28056111 | |
| successful salvage and reconstruction of a finger threatened by vibrio vulnificus necrotising fasciitis using fenestrated-type artificial dermis and three steps of topical negative pressure wound therapy. | vibrio vulnificus can cause severe skin and soft tissue infection (ssti). the pathogen is an opportunistic marine bacterium that is likely to infect patients with chronic liver disease, patients in an immunocompromised state, and those in end-stage renal disease. v. vulnificus gains entry through soft tissues by direct penetration of a wound by infected marine organisms, such as raw oysters, shellfish and other seafood, or by exposing a wound to contaminated water. despite its ease of entry, v. ... | 2017 | 28052529 |
| functional implications of hexameric assembly of rraa proteins from vibrio vulnificus. | rnase e has a pivotal role in the degradation and processing of rnas in escherichia coli, and protein inhibitors rraa and rrab control its enzymatic activity. the halophilic pathogenic bacterium vibrio vulnificus also expresses orthologs of rnase e and rraa-rnase ev, rraav1, and rraav2 (herein renamed as vvrnase e, vvrraa1, and vvrraa2). a previous study showed that vvrraa1 actively inhibits the ribonucleolytic activity of vvrnase e by interacting with the c-terminal region of vvrnase e. however ... | 2017 | 29261778 |
| cytotoxic and antibacterial compounds from the coral-derived fungus aspergillus tritici sp2-8-1. | three novel compounds, 4-methyl-candidusin a (1), aspetritone a (2) and aspetritone b (3), were obtained from the culture of a coral-derived fungus aspergillus tritici sp2-8-1, together with fifteen known compounds (4-18). their structures, including absolute configurations, were assigned based on nmr, ms, and time-dependent density functional theory (td-dft) ecd calculations. compounds 2 and 5 exhibited better activities against methicillin-resistant strains of s. aureus (mrsa) atcc 43300 and m ... | 2017 | 29112138 |
| antimicrobial peptide-loaded gold nanoparticle-dna aptamer conjugates as highly effective antibacterial therapeutics against vibrio vulnificus. | vibrio vulnificus causes fatal infections in humans, and antibiotics are commonly used in treatment regimens against v. vulnificus infection. however, the therapeutic effects of antibiotics are limited by multidrug resistance. in this study, we demonstrated that an antimicrobial peptide (amp), hpa3phis, loaded onto a gold nanoparticle-dna aptamer (aunp-apt) conjugate (aunp-apt-hpa3phis) is an effective therapeutic tool against v. vulnificus infection in vivo in mice. hpa3phis induced bacterial c ... | 2017 | 29051620 |
| isolation, identification and phenotypic and molecular characterization of pathogenic vibrio vulnificus isolated from litopenaeus vannamei. | the morphology and the drug sensitivity of the strain gyx2014-1 isolated from the hepatic pancreatic tissue of moribund litopenaeus vannamei were evaluated by conventional culture characteristics, physical and chemical characteristics, and molecular biology methods. detection of extracellulase and hemolysin activity shows that the isolated gyx2014-1 has protease, lipase, gelatinase activity, but none of amylase, or lecithinase activity. the 16s rrna gene (genbank accession number: kt781675) was ... | 2017 | 29045415 |
| vibrio vulnificus: new insights into a deadly opportunistic pathogen. | vibrio vulnificus is a gram-negative aquatic bacterium first isolated by the united states (us) centers for disease control and prevention (cdc) in 1964. this bacterium is part of the normal microbiota of estuarine waters and occurs in high numbers in molluscan shellfish around the world, particularly in warmer months. infections in humans are derived from consumption of seafood produce and from water exposure. vibrio vulnificus is a striking and enigmatic human pathogen, yet many aspects relate ... | 2017 | 29027375 |
| a case of vibrio vulnificus infection complicated with fulminant purpura: gene and biotype analysis of the pathogen. | introduction.vibrio vulnificus (v. vulnificus) causes a severe infection that develops in the compromised host. its pathophysiology is classified into three types: (1) primary septicaemia, (2) gastrointestinal illness pattern and (3) wound infection pattern. of these, primary septicaemia is critical. v. vulnificus can be classified into three biotypes and two genotypes and its pathogenicity is type-dependent. case presentation. a 47-year-old man presented to a local hospital with chief complaint ... | 2017 | 29026623 |
| efficacy of ceftriaxone, cefepime, doxycycline, ciprofloxacin, and combination therapy for vibrio vulnificus foodborne septicemia. | foodborne vibrio vulnificus infections are associated with higher rates of sepsis and mortality than wound infections; however, antibiotic efficacy studies have not been performed in foodborne infection models. the efficacies of ceftriaxone, cefepime, doxycycline, ciprofloxacin, and combination therapy were assessed in v. vulnificus intestinal infection in mice in order to model foodborne infections. in accordance with prior studies of cefotaxime, cefepime was synergistic with doxycycline and ci ... | 2017 | 28971862 |
| rapid biosynthesized agnps from gelidiella acerosa aqueous extract mitigates quorum sensing mediated biofilm formation of vibrio species-an in vitro and in vivo approach. | the present study explores the non-bactericidal anti-virulence efficacy of green synthesized silver nanoparticles (agnps) from gelidiella acerosa against multi-drug resistant vibrio spp. spectral characterization of agnps was performed through uv-visible, ft-ir, and energy-dispersive spectroscopic techniques followed by x-ray crystallography and zeta potential analysis. further, the structural characterization was done by electron and atomic force microscopic techniques. agnps profoundly quelled ... | 2017 | 28965300 |
| vibrio species involved in seafood-borne outbreaks (vibrio cholerae, v. parahaemolyticus and v. vulnificus): review of microbiological versus recent molecular detection methods in seafood products. | seafood products are widely consumed all around the world and play a significant role on the economic market. bacteria of the vibrio genus can contaminate seafood and thus pose a risk to human health. three main vibrio species, v. cholerae, v. parahaemolyticus and v. vulnificus, are potentially pathogenic to humans. these species are responsible for a dramatic increase of seafood-borne infections worldwide. hence, early detection of total and pathogenic vibrio is needed and should rely on quick ... | 2017 | 28956623 |
| occurrence of virulence genes associated with human pathogenic vibrios isolated from two commercial dusky kob (argyrosmus japonicus) farms and kareiga estuary in the eastern cape province, south africa. | background: seafood-borne vibrio infections, often linked to contaminated seafood and water, are of increasing global public health concern. the aim of this study was to evaluate the prevalence of human pathogenic vibrios and their associated virulence genes isolated from fish and water samples from 2 commercial dusky kob farms and kareiga estuary, south africa. methods: a total of 200 samples including dusky kob fish (n = 120) and seawater (n = 80) were subjected to vibrio screening on thiosulf ... | 2017 | 28946684 |
| morphological characterization and biocontrol effects of vibrio vulnificus phages against vibriosis in the shrimp aquaculture environment. | the re-emerging field of phage therapy is the potential biocontrol agents for the transfer of virulence factor and to kill their bacterial hosts. in this study, the lytic vibrio vulnificus phages were studied to provide a better understanding of phage-host interactions and development of phage therapy. four new v. vulnificus phages were detected from shrimp aquaculture system, named vv1, vv2, vv3 and vv4. all lytic v. vulnificus phages are the tectiviruses of the family tectiviridae with typical ... | 2017 | 28917670 |
| biochemical characterization of a catalase from vibrio vulnificus, a pathogen that causes gastroenteritis. | vibrio vulnificus is a virulent human pathogen causing gastroenteritis and possibly life threatening septicemia in patients. most v. vulnificus are catalase positive and can deactivate peroxides, thus allowing them to survive within the host. in the study presented here, a catalase from v. vulnificus (cat-vv) was purified to homogeneity after expression in escherichia coli. the kinetics and function of cat-vv were examined. cat-vv catalyzed the reduction of h2o2 at an optimal ph of 7.5 and tempe ... | 2017 | 28892106 |
| proteomics and 1h nmr-based metabolomics analysis of pathogenic vibrio vulnificus aquacultures isolated from sewage drains. | vibrio bacteria live in both marine and freshwater habitats and are associated with aquatic animals. vibrio vulnificus is a pathogenic bacterium that infects people and livestock. it is usually found in offshore waters or within fish and shellfish. this study presents a comparative proteomic analysis of the outer membrane protein (omp) changes in v. vulnificus proteins after stimulation with sewage from sewage drains. using two-dimensional electrophoresis followed by maldi-tof ms/ms, 32 protein ... | 2017 | 28864971 |
| exogenous polyunsaturated fatty acids impact membrane remodeling and affect virulence phenotypes among pathogenic vibrio species. | the pathogenic vibrio species (v. cholerae, v. parahaemolyticus, and v. vulnificus) represent a constant threat to human health, causing foodborne and skin wound infections as a result of ingestion of or exposure to contaminated water and seafood. recent studies have highlighted vibrio's ability to acquire fatty acids from environmental sources and assimilate them into cell membranes. the possession and conservation of such machinery provokes consideration of fatty acids as important factors in ... | 2017 | 28864654 |
| complete genome sequence of the pathogenic vibrio vulnificus type strain atcc 27562. | vibrio vulnificus has the highest death rate and economic burden per case of any foodborne pathogen in the united states. a complete genome sequence of the type strain promotes comparative analyses with other clinical and environmental isolates, improving our understanding of this important human pathogen and successful environmental organism. | 2017 | 28860258 |
| importance of fumarate and nitrate reduction regulatory protein for intestinal proliferation of vibrio vulnificus. | the sepsis caused by vibrio vulnificus is characterized by an average incubation period of 26 h and a high mortality rate exceeding 50%. the fast growth and dissemination of v. vulnificus in vivo lead to poor clinical outcomes in patients. therefore, elucidation of the proliferation mechanisms of this organism in vivo may lead to the development of an effective therapeutic strategy. in this study, we focused on the low oxygen concentration in the intestinal milieu because of its drastic differen ... | 2017 | 27915250 |
| rapid identification of pathogens responsible for necrotizing fasciitis on an integrated microfluidic system. | necrotic fasciitis (nf) is a particularly aggressive and serious infection of the fascia that can penetrate into the musculature and internal organs, resulting in death if not treated promptly. in this work, an integrated microfluidic system composed of micropumps, microvalves, and micromixers was used to automate the detection of pathogens associated with nf. the entire molecular diagnostic process, including bacteria isolation, lysis, nucleic acid amplification and optical detection steps, was ... | 2017 | 29282420 |
| wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution. | fish skin mucosal surfaces (sms) are quite similar in composition and function to some mammalian ms and, in consequence, could constitute an adequate niche for the evolution of mucosal aquatic pathogens in natural environments. we aimed to test this hypothesis by searching for metagenomic and genomic evidences in the sms-microbiome of a model fish species (anguilla anguilla or eel), from different ecosystems (four natural environments of different water salinity and one eel farm) as well as the ... | 2017 | 29268781 |
| a conserved tad pilus promotes vibrio vulnificus oyster colonization. | vibrio vulnificus has the highest death rate (>35%) and per-case economic burden ($3.3 million) of any foodborne pathogen in the united states. infections occur via open wounds or following ingestion of contaminated seafood, most infamously oysters. we isolated a 1000th generation descendant, designated nt that exhibited increased biofilm and aggregate formation relative to its parent. we identified two significant causal changes underlying these phenotypes. first, the entire 24-kb capsular poly ... | 2017 | 29235706 |
| [amputation of an arm due to infection with vibrio vulnificus after beach holiday]. | infection with vibrio vulnificus is a rare condition with approximately 11 cases reported in the danish literature. it is characterized by soft tissue infection/wound, necrotizing fasciitis and septicaemia. in this case report we present a patient admitted with a rapid progression and sepsis consistent with v. vulnificus infection but with no informa-tion of water exposure. the initial treatment was surgery and sepsis management including broad-spectrum antibiotics. on day eight the patient's ri ... | 2017 | 29208199 |
| complete genome sequence of the vibrio vulnificus strain vv2014djh, a human-pathogenic bacterium isolated from a death case in china. | vibrio vulnificus, an opportunistic pathogen, is the causative agent of life-threatening septicemia and severe wound infections. however, the pathogenicity and virulence factors of v. vulnificus are not fully understood. here we report the complete genome sequence of v. vulnificus vv2014djh, which was isolated from a death case. | 2017 | 29201149 |
| analysis of the bacterial microbiome in the small octopus, octopus variabilis, from south korea to detect the potential risk of foodborne illness and to improve product management. | the small octopus (octopus variabilis) is a popular seafood in many countries including south korea. because it is often consumed uncooked, the microorganisms in it often cause food poisoning. therefore, analyzing the microbiome of the small octopus can help to understand the risk of food poisoning and manage octopus products better. a total of 40 small octopuses were collected from four sites in november and august. the microbiota was analyzed using illumina miseq sequencing, and the amount of ... | 2017 | 29195979 |
| bioactive pimarane diterpenes from the arctic fungus eutypella sp. d-1. | two new pimarane diterpenes, libertellenone m (1) and libertellenone n (2), together with five known compounds were isolated from the culture extract of eutypella sp. d-1 derived from high-latitude soil of the arctic. the structures of these compounds were determined by spectroscopic data as well as experimental and calculated electronic circular dichroism (ecd) analysis. antimicrobial and cytotoxic activities of the isolated compounds were evaluated. compound 3 exhibited weak antibacterial acti ... | 2017 | 29168349 |
| the opportunistic pathogen vibrio vulnificus produces outer membrane vesicles in a spatially distinct manner related to capsular polysaccharide. | vibrio vulnificus, a bacterial species that inhabits brackish waters, is an opportunistic pathogen of humans. v. vulnificus infections can cause acute gastroenteritis, invasive septicemia, tissue necrosis, and potentially death. virulence factors associated with v. vulnificus include the capsular polysaccharide (cps), lipopolysaccharide, flagellum, pili, and outer membrane vesicles (omvs). the aims of this study were to characterize the morphology of v. vulnificus cells and the formation and arr ... | 2017 | 29163452 |
| site-specific his/asp phosphoproteomic analysis of prokaryotes reveals putative targets for drug resistance. | phosphorylation of amino acid residues on proteins is an important and common post-translational modification in both eukaryotes and prokaryotes. most research work has been focused on phosphorylation of serine, threonine or tyrosine residues, whereas phosphorylation of other amino acids are significantly less clear due to the controversy on their stability under standard bioanalytical conditions. | 2017 | 28545444 |
| previously undescribed antibacterial polyketides from heterotrophic bacillus amyloliquefaciens associated with seaweed padina gymnospora. | a heterotrophic marine bacterium bacillus amyloliquefaciens isolated from seaweed padina gymnospora exhibited broad spectra of antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio harveyi, vibrio vulnificus, and vibrio parahaemolyticus. the seaweed-associated b. amyloliquefaciens was recognized to possess functional type i polyketide synthase-1 (pks-1) gene, and was used to isolate four homologous compounds with polyketide frameworks. the compounds were characterized ... | 2017 | 28842846 |
| corrigendum: assessing the function of stas domain protein sypa in vibrio fischeri using a comparative analysis. | [this corrects the article on p. 760 in vol. 6, pmid: 26284045.]. | 2017 | 28450861 |
| anti-biofilm effects of anthranilate on a broad range of bacteria. | anthranilate, one of tryptophan degradation products has been reported to interfere with biofilm formation by pseudomonas aeruginosa. here, we investigated the effects of anthranilate on biofilm formation by various bacteria and the mechanisms responsible. anthranilate commonly inhibited biofilm formation by p. aeruginosa, vibrio vulnificus, bacillus subtilis, salmonella enterica serovar typhimurium, and staphylococcus aureus, and disrupted biofilms preformed by these bacteria. because anthranil ... | 2017 | 28819217 |
| recreational waterborne illnesses: recognition, treatment, and prevention. | illness after recreational water activities can be caused by a variety of agents, including bacteria, viruses, parasites, algae, and even chlorine gas. these illnesses are more common in summer. waterborne illnesses are underreported because most recreational activity occurs in unsupervised venues or on private property, and participants tend to disperse before illness occurs. symptoms of waterborne illness are primarily gastrointestinal, but upper respiratory and skin manifestations also occur. ... | 2017 | 28671392 |
| occurrence of vibrio species, beta-lactam resistant vibrio species, and indicator bacteria in ballast and port waters of a tropical harbor. | ballast water discharges are potential sources for the spread of invasive and pathogenic aquatic organisms. ballast waters from six ships docked in the port of singapore were tested to determine if indictor organisms fell within proposed standards for ballast water discharge according to regulation d-2 of the ballast water management convention (bwmc) guidelines. vibrio species were cultured on media supplemented with beta-lactam antibiotics to determine the presence of antibiotic resistant vibr ... | 2017 | 28822933 |
| effects of desiccation practices of cultured atlantic oysters (crassostrea virginica) on vibrio spp. in portersville bay, alabama, usa. | the expansion of off-bottom aquaculture to the gulf of mexico has raised public health concerns for human health officials. high temperatures in the gulf of mexico are associated with high levels of vibrio parahaemolyticus and vibrio vulnificus. routine desiccation practices associated with off-bottom aquaculture expose oysters to ambient air, allowing vibrio spp. to proliferate in the closed oyster. currently, there is limited research on the length of time needed for vibrio spp. levels in desi ... | 2017 | 28696147 |