Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| direct effects of alpha 2-adrenergic receptor stimulation on intravascular systemic capacity in the dog. | the role of alpha 2-adrenergic receptor stimulation in the regulation of systemic vascular capacity and venous return, a major determinant of cardiac output, is not well understood. with the influence of the central nervous system isolated from the systemic circulation, the direct peripheral vascular effects of two specific, chemically distinct alpha 2-adrenergic receptor agonists, uk 14,304 and b-ht 920, were investigated in 19 dogs on total cardiopulmonary bypass with constant arterial perfusi ... | 1988 | 2895736 |
| bacterial diarrhoea and treatment. | 1988 | 2893218 | |
| sex as a risk factor for htlv-i spread among intravenous drug abusers. | 1988 | 2895250 | |
| [bovine herpesvirus type 2 infection]. | 1988 | 2845879 | |
| [specificity of the protective action of a ribosomal shigella vaccine and the absence of activity in the ribosomes from r mutants]. | the ribosomal preparations of s. sonnei and some other bacterial species were obtained by the method of differential centrifugation, and the specificity of their protective action was studied in the keratoconjunctivitis test on guinea pigs. the ribosomal preparations were introduced parenterally in a single injection, and their protective action was determined two weeks later by the challenge of the animals with s. sonnei virulent strain and the subsequent calculation of the efficiency index (ei ... | 1988 | 2464261 |
| [lipid peroxidation in experimental viral infection and the effect on it of interferons and alpha-tocopherol]. | lipid peroxidation as well as effects of various interferons (native, from fibroblasts of new born and adult animals) on the peroxidation were studied in blood plasma, erythrocytes, liver and kidney tissues of mice with viral infection. these interferons, simultaneously with antiviral action, exhibited the positive effect on lipid peroxidation, while the interferon from fibroblasts of newborns produced the side reactions. simultaneous use of the interferon and alpha-tocopherol removed these side ... | 1988 | 2467439 |
| [the action of azepoxole on central alpha 2-adrenoreceptors]. | the effect of adrenomimetics on the central alpha 2-adrenoreceptors responsible for mydriasis was studied. it was found that alpha 2-adrenomimetic azepoxole (bht-933) exerts no effect on the central alpha 2-adrenoreceptors providing mydriasis. | 1988 | 2906881 |
| heterogeneity in chromosome damage and repair rates after bleomycin in ataxia telangiectasia cells. | cells derived from patients with ataxia telangiectasia (at) are known to be exceptionally sensitive to ionizing radiation and chemotherapeutic agents such as bleomycin (blm), neocarzinostatin, and etoposide. this increased sensitivity is manifested by high chromosome aberration frequencies after treatment. in order to probe the underlying basis for this phenomenon, the technique of premature chromosome condensation was used to determine whether the increased chromosome damage observed after bleo ... | 1988 | 2446745 |
| reducing the illegal sale of cigarettes to minors. | this study reports on an effort to stop the illegal sale of cigarettes to minors. in santa clara county, calif, 412 stores and 30 vending machines were visited by 18 minors aged 14 through 16 years with the intent to purchase cigarettes; they were successful at 74% of the stores and 100% of the vending machines. after an aggressive six-month campaign using communitywide media, direct merchant education, contact with the chief executive officers of chain stores and franchise operations owned by m ... | 1989 | 2908999 |
| effect of aging on populations of estrogen receptor-containing cells in the rat uterus. | estrogen receptor-containing cells were identified in uteri of mature and senescent rats by means of quantitative autoradiography. cell numbers and density were assessed in the various uterine layers. it was determined that despite decreases in receptor concentrations in endometrium, endometrial stroma, myometrium, and epithelium, no significant cell loss occurred in any region. receptor content per cell clearly decreased with age in the endometrial stroma, while only in the myometrium was there ... | 1989 | 2909390 |
| protective role of serum antibody in immunity to chlamydial genital infection. | female guinea pigs were injected intraperitoneally with pooled immunoglobulin derived from animals immunized to the chlamydial agent of guinea pig inclusion conjunctivitis. genital infections in animals receiving pooled immunoglobulin from immune animals were markedly decreased with regard to the number of inclusions detected compared with control animals. these data indicated that serum-derived antibody was able to provide a degree of protection against a chlamydial genital tract infection. | 1989 | 2909492 |
| newer techniques of laryngeal reinnervation. | we relate the experience obtained in the use of the right superior laryngeal nerve (motor branch)--cricothyroid muscle pedicle flap in dogs in an attempt to reinnervate the right posterior cricoarytenoid muscle (pca). the right vocal cord was paralyzed by severance and removal of 2.5 cm of the right recurrent laryngeal nerve. evaluation 6 months postoperatively revealed the vocal fold remobilization on the right side to have an average of about one half the mobility of the left, normal side. aft ... | 1989 | 2910195 |
| polyclonal anti-idiotypes influence macrophage chemotaxis in coxsackievirus-induced murine myocarditis. | investigation into the mechanism(s) whereby monocytes-macrophages are mobilized to the myocardium of coxsackievirus (cvb3)-infected mice was approached by assessing the role of anti-idiotypic antibodies against specific antiviral igg molecules. balb/c mice preinoculated with polyclonal anti-idiotypic, syngeneic antibodies resulted in significantly decreased myocarditis after virus challenge. on the other hand, oil-induced peritoneal exudate cells obtained from virus-exposed animals demonstrated ... | 1989 | 2911021 |
| polyamines are sufficient to drive the transport of the precursor of ornithine carbamoyltransferase into rat liver mitochondria: possible effect on mitochondrial membranes. | the polyamines spermidine, spermine and putrescine, by themselves, at physiological concentrations, induce the transport of the precursor of ornithine carbamoyltransferase into isolated rat liver mitochondria. the presence of polyamines in the transport medium results in the approach of both mitochondrial membranes, suggesting a possible role of these molecules in the transport of the precursor of ornithine carbamoyltransferase into mitochondria, by the formation and/or stabilization of mitochon ... | 1989 | 2912450 |
| failure of vitamin e to protect the retina against damage resulting from bright cyclic light exposure. | cumulative light-mediated damage to the retina over a long time period may be involved in the development of age-related retinopathies. light is thought to produce retinal damage by initiating autoxidative reactions among the molecular components of the retina. experiments were therefore conducted (1) to confirm that long-term differences in cyclic light intensity affect the rate of age-related photoreceptor cell loss from the retina; and (2) to determine whether the antioxidant, vitamin e, is a ... | 1989 | 2912912 |
| aplysia synaptosomes. i. preparation and biochemical and morphological characterization of subcellular membrane fractions. | we prepared and characterized subcellular membrane fractions from the cns of aplysia californica that are enriched in isolated nerve terminals (synaptosomes). ganglia were homogenized in 1.1 m sucrose and fractionated on a 2-step sucrose gradient, yielding 50 micrograms protein/animal in the synaptosomal fraction (p3), which was enriched 3-fold in plasma membrane as compared with the initial homogenate. quantitative morphometry of electron micrographs revealed that p3 contained 25% intact synapt ... | 1989 | 2913212 |
| improved sexual function in hemodialysis patients on recombinant erythropoietin: a possible role for prolactin. | as it was reported that correction of anemia in long-term hemodialysis patients by recombinant human erythropoietin (r-huepo) is associated with improved sexual function, we conducted the present study to further delineate the mechanism(s) by which this is brought about. serum prolactin, testosterone, and parathyroid hormone (pth) levels were followed during 4 months of r-huepo therapy. within 4 months of treatment with r-huepo, hematocrit values rose from 23.7 +/- 1.2 to 35.7 +/- 0.2% and hemog ... | 1989 | 2914405 |
| the protective effect of thromboxane synthetase inhibition on renal function in systemic sepsis. | to study the role of thromboxane in systemic sepsis and renal failure, peritonitis was induced surgically in 22 sheep, leading to local and systemic sepsis. a selective thromboxane synthetase inhibitor, u63,557a (upjohn co, kalamazoo, mi) was given before surgery in five animals and 30 minutes after surgery in five animals. a typical picture of volume-loaded, normotensive, vasodilated septic shock developed in all animals. twenty four hours after induction of sepsis, the control group showed a m ... | 1989 | 2916566 |
| ph-dependent uptake and macrofilaricidal effects of chloroquine on adult filarial parasites in vitro. | uptake and macrofilaricidal effects of chloroquine (cq) and other aminoquinolines were found to be highly ph dependent in brugia pahangi, acanthocheilonema viteae, onchocerca volvulus, and dirofilaria immitis. using [3h]cq, it was found that all of the parasites took up more drug under alkaline conditions (rpmi 1640 at ph 8.4) than in neutral (ph 7.4) or acidic (ph 6.8) media. differences were seen in the amount of drug taken up among the filariae studied. b. pahangi and a. viteae took up 7 time ... | 1989 | 2917628 |
| hepatic structural correlates of liver fibrosis: a morphometric analysis. | liver fibrosis is an important feature of many liver diseases, and the assessment of fibrosis is essential for diagnosis and prognosis. liver needle biopsy, however, tends to sample preferentially the soft parenchyma, which poorly reflects the true extent of fibrosis. therefore, we have searched for capsule and parenchymal features that are correlated with the fibrosis. to test for these correlations, we used a morphometric analysis of a rat model of liver fibrosis. we demonstrated that, of the ... | 1989 | 2920817 |
| degradation of type ix collagen by matrix metalloproteinase 3 (stromelysin) from human rheumatoid synovial cells. | the degradation of type ix collagen, a minor collagen in cartilage, was examined by treatment with three different types of matrix metalloproteinases (mmps) purified from the culture medium of rheumatoid synovial cells. neither mmp-1 (collagenase) nor mmp-2 (so-called 'gelatinase') could digest type ix collagen, but mmp-3 (stromelysin) readily degraded it into smaller fragments. this suggests that mmp-3 may be responsible for the pathological degradation and/or normal turnover of type ix collage ... | 1989 | 2920840 |
| sensitivity of campylobacter pylori to colloidal bismuth subcitrate. | 1989 | 2921366 | |
| [comparative diagnostic value of methods for detecting dysentery antigens in substrates of the patient's body]. | the comparative evaluation of different immunological methods, such as the enzyme immunoassay, the aggregate hemagglutination test and the complement fixation test, used for the detection of specific shigella antigens in biological body substrates obtained from 287 patients with acute dysentery caused by s. sonnei, s. flexneri and s. newcastle has been carried out. the enzyme immunoassay and the aggregate hemagglutination test most effective (97.5 +/- 0.5 and 92.4 +/- 0.9, respectively), the obj ... | 1989 | 2469267 |
| human milk immunoglobulin a antibodies to shigella virulence determinants. | because human milk is thought to protect infants from shigellosis, we evaluated milk for immunoglobulin a to shigella virulence determinants. milk was preincubated to remove antibodies unrelated to each locus of interest, using defined shigella and e. coli hybrids containing known shigella genetic segments prior to immunoblotting. the milk could not be shown to contain antibodies to chromosomally encoded virulence loci except for the expected antibodies to the products of the histidine locus. ho ... | 1989 | 2470676 |
| in vitro treatment of hep-2 cells with human tumor necrosis factor-alpha and human interferons reduces invasiveness of salmonella typhimurium. | enteroinvasive bacteria, like salmonella typhimurium, can be internalized in in vitro cultured epithelioidal cells, like hep-2 cells. this phenomenon is inhibited by pretreatment of cells with human tumor necrosis factor alpha (tnf-alpha) in a dose- and time-dependent manner. the effect was also reproduced in other cell types, including diploid embryo fibroblast cells. the tnf-alpha effect was neutralized by anti-tnf-alpha antibodies. no synergistic effect was produced by combinations of tnf-alp ... | 1989 | 2472042 |
| expression in escherichia coli k-12 of the 76,000-dalton iron-regulated outer membrane protein of shigella flexneri confers sensitivity to cloacin df13 in the absence of shigella o antigen. | one of the chromosomal segments associated with virulence in shigella flexneri encodes the production of aerobactin and the synthesis of an iron-regulated 76-kilodalton outer membrane protein believed to be the ferric-aerobactin receptor. however, s. flexneri expressing this putative aerobactin receptor, which is slightly larger than that encoded by pcolv, is insensitive to the killing action of cloacin df13, a bacteriocin which binds to other aerobactin receptor proteins and kills the cells. in ... | 1989 | 2474501 |
| a dual transcriptional activation system for the 230 kb plasmid genes coding for virulence-associated antigens of shigella flexneri. | the expression of plasmid-encoded, invasion-related antigens lpa b, c and d of shigella flexneri was found to be positively regulated at transcriptional level by a 33kd protein produced by the previously defined, virulence-associated region 1 on the sali fragment b of the 230 kb invasion plasmid. the gene (designated virb) was identified and its nucleotide sequence determined. no ipa b or c was produced in the absence of an intact virb gene although lower levels of d were produced. the previousl ... | 1989 | 2474742 |
| identification of a 2-md plasmid from shigella flexneri associated with reactive arthritis. | the development of reactive arthritis, a sterile inflammatory polyarthropathy that primarily affects hla-b27 positive individuals, has been associated with previous enteric infections caused by various gram-negative bacteria. the possibility that a common bacterial epitope triggers the disease was investigated by screening a panel of documented arthritogenic shigella strains as well as 2 epidemic-associated nonarthritogenic shigella controls. a 2-md plasmid specific to the arthritogenic strains ... | 1989 | 2475114 |
| identification and characterization of transcription termination sites in the escherichia coli lacz gene. | the escherichia coli lacz gene contains a series of latent transcriptional terminators that are responsible for the polar effects of certain mutations. we demonstrate, using gel electrophoretic size analyses and nuclease s1 mapping procedures, that rna polymerase terminates rna synthesis in the vicinity of five positions 180, 220, 379, 421 and 463 base-pairs downstream from the start point during transcription of lacz dna in vitro in the presence of rho factor. termination at all but the 421 pos ... | 1989 | 2475637 |
| antihypertensive, anticalcinotic, and antiarteriosclerotic properties of anipamil, a long-acting new derivative of verapamil. | the classical indication for calcium antagonists has for a long time been coronary heart disease. however, more recent experimental data as well as a host of clinical observations now encourage a broader use of these drugs also for antihypertensive purposes and for prevention of concomitant sclerotic vascular injury. needless to say, for the treatment of hypertensive crises rapidly acting calcium antagonists are required, whereas for chronic therapy of hypertension calcium antagonists with a lon ... | 1989 | 2475679 |
| [development and use of solid-phase test-systems for the detection of o-antigen of shigella sonnei and shigella flexneri]. | the possibility of the diagnosis of dysentery caused by s. sonnei and s. flexneri, as well as the determination of the dynamics of the distribution of specific o-antigen in the patient's body, by means of the enzyme-linked immunosorbent assay system developed on the basis of antibody preparations obtained by immunosorption has been studied. the study has shown that for better diagnosis the use of fecal extracts is preferable in assays; when used in combination with bacteriological analysis, thes ... | 1989 | 2477970 |
| [use of immunoenzyme analysis in the rapid diagnosis and study of the characteristics of circulating o-antigen in biological fluids of patients with acute dysentery sonne]. | the data on the clinical approval of the original enzyme immunoassay system for the determination of somatic o-antigen in the blood serum and urine of patients with acute sonne dysentery are presented. the level of the antigen determined in the biological fluids of patients has been shown to depend on the severity of the disease. different types of dynamic curves, reflecting the level of o-antigen in the biological fluids of patients with acute sonne dysentery and characteristic of different cli ... | 1989 | 2477971 |
| [cloning of the gene relative to invasive ability of shigella flexneri 5]. | a genomic library of s. flexneri 5 large plasmid (140 md) was constructed using the cosmid pjb8 as vector. there were more than 4,000 transformants in the library. 66 clones were picked out from the library by hybridization with 17kb gene probe relative to invasive ability. a few clones were analyzed. the results indicated that all these clones tested contained recombinant plasmids, they could hybridize with 17 kb gene probe. when these recombinant plasmids were digested with ecor1, 17 kb fragme ... | 1989 | 2486252 |
| mechanism of antigenic variation in shigella flexneri bacilli. i. selective lethal effect of normal sera on mixed population of s. flexneri 1b serotype and its antigenic 3b mutant. | normal non-immune calf, human and mice sera kill-preferentially the shigella flexneri 1b serotype cells with antigenic formula i; 3, 4, 6 leaving the antigenic mutant cells of the serotype 3b with antigenic formula iii; 3, 4, 6. the strongest lethal action was observed in normal calf serum. this serum makes possible to obtain the 3b serotypes mutant from pure population of 1b serotype cells. thermal inactivation of the complement deprives the serum of its selective activity. | 1989 | 2487379 |
| lack of effect by prostaglandin f2 alpha and verapamil on calcium uptake by isolated corpora lutea from pseudopregnant rats. | 1989 | 2492809 | |
| intracisternal injection of a trh analogue stimulates gastric luminal serotonin release in rats. | intracisternal injection of trh analogue, rx 77368, which has been previously shown to induce a vagal-dependent stimulation of gastric acid secretion and contractility dose-dependently (10-300 ng) stimulated serotonin release into the gastric lumen of conscious pylorus-ligated rats. the stimulation of gastric acid and serotonin release occurred 15 and 30 min, respectively, after intracisternal injection of rx 77368 (100 ng) and lasted for over 2 h, as measured in urethan-anesthetized rats with g ... | 1989 | 2493198 |
| renal function and morphology in sudanese patients with advanced hepatosplenic schistosomiasis and portal hypertension. | the association between glomerular disease and hepatosplenic schistosomiasis is well documented in reports from south america. during the present hospital investigation in sudan, 58 patients admitted for intercurrent complications of advanced hepatosplenic schistosomiasis were studied. the patients, median age 35 years, had no concurrent schistosoma haematobium infection. diagnostic criteria included an enlarged spleen (n = 58), at least 1 episode of hematemesis (n = 55) and/or melena (n = 36), ... | 1989 | 2493202 |
| in vitro susceptibility of feline herpesvirus-1 to vidarabine, idoxuridine, trifluridine, acyclovir, or bromovinyldeoxyuridine. | in vitro activities of 9-[( 2-hydroxyethoxy] methyl) guanine (acyclovir), (e)-5-(2-bromovinyl)-2'deoxyuridine, 9-beta-d-arabinofuranosyladenine (vidarabine), 5-iodo-2'-deoxyuridine (idoxuridine), and 5-trifluoromethyl-2'-deoxyuridine (trifluridine) were studied against 6 strains of feline herpesvirus-1. a significant difference was not detected among viral strains in their susceptibility to these compounds (p = 0.442). the relative potency of these compounds was trifluridine much greater than id ... | 1989 | 2493210 |
| [value of determining the spectrum of blood serum fatty acids in evaluating increasing the effectiveness of antibiotic therapy of dysentery in children with prodigiozan and ephedrine]. | one hundred and ninety one children with acute sonne and flexner dysentery were observed with respect to the disease process, immunity indices and blood serum fatty acid spectrum. 104 children were treated with monomycin alone and 87 children were treated with the antibiotic in combination with prodigiozan and ephedrine as immunostimulators. it was shown that the recovery terms in the patients treated with the use of the immunostimulators decreased as compared to the patients treated with the an ... | 1989 | 2502081 |
| hemagglutinating properties of shigella dysenteriae type 1 and other shigella species. | strains of shigella dysenteriae type 1 cultured in casamino acids-yeast extract broth medium in the presence of 1 mm calcium chloride at 37 degrees c for 22 h induced hemagglutination of erythrocytes that was inhibited by n-acetylneuraminic acid, n-acetylneuramin-lactose, and alpha 1-glycoprotein. the hemagglutination was heat labile, and the absence of cell-surface appendages suggested a nonfimbrial adhesin(s). under the same conditions, strains of shigella flexneri (types 1a, 1b, 2a, and 2b) s ... | 1989 | 2503449 |
| infections in british clinical laboratories, 1986-87. | during 1986-87 this continuing survey showed 15 specific infections in the staff of 235 laboratories, representing 28,524 person years of exposure. the community was the probable source of four of the five cases of tuberculosis and one of the five cases of salmonellosis. occupational exposure was the probable cause of four infections by shigella flexneri, three by salmonella typhimurium, and one by s typhi, all affecting medical laboratory scientific officers (mlsos) in microbiology. occupationa ... | 1989 | 2503546 |
| [clinical studies on patients with legal communicable diseases encountered from 1981 to 1986]. | patients with legal communicable diseases admitted to kawasaki municipal hospital between 1981 and 1986 were studied. ages of patients were over 15 year-old. during this period 115 patients were admitted. out of these patients 84 had shigellosis, and 9 had amebiasis. out of 20 salmonellosis, 18 were due to salmonella typhi and 2 were due to salmonella paratyphi-a, respectively. two were admitted because of cholera. out of the patients with shigellosis, 27 were domestic and 57 were foreign infect ... | 1989 | 2504839 |
| [the isolation and immunologic study of ribosomal preparations from shigella flexneri]. | s. flexneri ribosomal preparations were isolated by differential centrifugation or by fractionation with polyethylene glycol-6000. their chemical composition and spectrophotometric properties were characteristic of ribosomes, and, as shown by the results of the serological assay, the content of o-specific component was, on the average, 1.4%. the ribosomal preparations were nontoxic for mice when injected intraperitoneally and intravenously in large doses and induced systemic o-antibody response ... | 1989 | 2511706 |
| [in vitro susceptibility of campylobacter jejuni and campylobacter coli to quinolone antibiotics]. | in this study, in vitro susceptibilities of 24 campylobacter strains isolated from fecal samples were tested for quinolone antibiotics. 13 of the strains were identified as c. jejuni and 11 of them were c. coli. antibiotics which were included in this study were ciprofloxacin, ofloxacin, pefloxacin and fleroxacin. antibiotic susceptibility tests were performed on mueller-hinton agar (supplemented with 5% sheep blood) using the agar dilution method. results showed that quinolone antibiotics are v ... | 1989 | 2518646 |
| [in vitro cultivation of human pulpal fibroblast strains--permanent and deciduous teeth]. | we succeeded in separating and the cultivating stable monolayer cultures of dental pulp fibroblast strains derived from permanent and deciduous human teeth. human permanent (n = 67) and deciduous teeth (n = 26) were extracted under acupuncture anaesthesia for the correction of malocclusion. after splitting the teeth, the pulp tissues were carefully removed, placed in tissue culture flasks, and grown in dulbecco's modified eagle medium supplemented with 10% fetal calf serum (fcs). the human pulpa ... | 1989 | 2519279 |
| [uropathogenic serratia marcescens of hospital origin: analysis of the adhesion mechanism of 2 strains]. | we isolated 2 serratia marcescens strains, fim (0) and fim (-), from patients with urinary tract infection. adhesion properties were studied by hemagglutination, response of uroepithelial cells and electron microscopy techniques. s marcescens fim (+) showed high adherence to uroepithelial cells, hemagglutination capacity and peculiar electron microscopy characteristics. all of these are absent in the fim (-) variety. the fim (+) strain was resistant to nitrofurantoin, nalidixic acid, tetracyclin ... | 1989 | 2519411 |
| bidimensional reversed-phase high-pressure liquid chromatography analysis of cultured cell neuropeptides: application to atrial natriuretic factor. | we report here a one-step procedure for extraction and analysis of neuropeptides in chromaffin cell culture media and acid extracts using reversed-phase hplc. the bidimensional hplc system consists of a precolumn connected to a six-port switching valve which is on-line with an analytical column. the direct injection of the biological samples onto the precolumn previously equilibrated with 15% acetonitrile allows the elimination of interfering substances. the samples purified on the precolumn can ... | 1989 | 2527010 |
| determination of the secondary structure of the dna binding protein ner from phage mu using 1h homonuclear and 15n-1h heteronuclear nmr spectroscopy. | the sequential resonance assignment of the 1h and 15n nmr spectra of the dna binding protein ner from phage mu is presented. this is carried out by using a combination of 1h-1h and 1h-15n two-dimensional experiments. the availability of completely labeled 15n protein enabled us to record a variety of relayed heteronuclear multiple quantum coherence experiments, thereby enabling the correlation of proton-proton through-space and through-bond connectivities with the chemical shift of the directly ... | 1989 | 2527559 |
| immunogenicity of a yeast-derived hepatitis b vaccine in hemodialysis patients. | in a multicenter study of hemodialysis patients in spain, the immunogenicity of a yeast-derived recombinant deoxyribonucleic acid hepatitis b vaccine was evaluated. two different vaccination schedules were examined: zero, one, two, six months and zero, one, two, 12 months. two different dose levels (20 micrograms and 40 micrograms) were also compared. no serious adverse effects were reported by any of the vaccinees; the most frequently reported reaction was soreness at the injection site. this s ... | 1989 | 2528295 |
| 1-(3-trifluoromethylphenyl) piperazine (tfmpp) in the ventral tegmental area reduces the effect of desipramine in the forced swimming test in rats: possible role of serotonin receptors. | 1-(3-trifluoromethylphenyl)piperazine (tfmpp), a serotonin1 (5-ht1) receptor agonist, injected i.p. in doses of 0.1 and 0.6 mg/kg, did not modify the immobility time of rats in the forced swimming test but significantly antagonized the effect of a 7 days treatment with 10 mg/kg per day desipramine (dmi). a similar effect was found on infusing 1 and 5 micrograms/microliters tfmpp bilaterally into the ventral tegmental area (vta). infusion of 5 micrograms/microliters tfmpp into the nucleus accumbe ... | 1989 | 2533079 |
| identification of icsa, a plasmid locus of shigella flexneri that governs bacterial intra- and intercellular spread through interaction with f-actin. | the capacity of shigella to spread within the cytosol of infected epithelial cells and to infect adjacent cells is critical for the development of infection foci, which lead to mucosal abscesses. shigella is a nonmotile microorganism that appears to utilize host cell microfilaments to generate intra- as well as intercellular movements, since this movement was inhibited by cytochalasin d and involvement of f-actin was demonstrated by direct labeling of infected cells with the specific dye n-(7-ni ... | 1989 | 2542950 |
| human endothelial cells inhibit platelet aggregation by separately stimulating platelet cyclic amp and cyclic gmp. | thrombin-induced platelet aggregation was monitored in a mixture of washed human platelets and cultured human endothelial cells on microcarrier beads. endothelial cells completely inhibited platelet aggregation and enhanced the content of both cyclic amp and cyclic gmp in the platelets. inhibition of endothelial prostacyclin synthesis with indomethacin abolished the cyclic amp increase in the platelets, whereas the cyclic gmp increase was unimpaired. a significant component of the endothelial an ... | 1989 | 2546782 |
| characterization of cdna clones encoding two putative isoforms of the alpha 1 subunit of the dihydropyridine-sensitive voltage-dependent calcium channel isolated from rat brain and rat aorta. | cdna clones encoding rat brain and rat aorta isoforms of the alpha 1 subunit of the dihydropyridine-sensitive, voltage-dependent calcium channel were isolated and sequenced. these tissue-specific cdna clones share significant amino acid similarity with the rabbit skeletal muscle calcium channel alpha 1 subunit (75% and 66% amino acid identity for rat brain and rat aorta isoforms, respectively). northern analysis revealed transcript sizes of 6.5 and 8.6 kb in aorta and 8.6 kb in brain. | 1989 | 2546805 |
| influence of non-esterified fatty acids on respiratory control of reconstituted cytochrome-c oxidase. | bovine heart cytochrome-c oxidase was reconstituted in liposomes (asolectin) and the activity measured in the presence and absence of uncoupler at increasing concentrations of non-esterified fatty acids. palmitic and stearic acids resulted in a decrease of about 40% in the respiratory control ratio at a concentration of 1 microm, when measured using a spectrophotometric procedure but not with a polarographic assay method. at higher fatty acid concentrations no further change was found. a 50% dec ... | 1989 | 2546826 |
| isolation and characterisation of calcineurin from adrenal cell cytoskeleton: identification of substrates for ca2+-calmodulin-dependent phosphatase activity. | ca2+-calmodulin-dependent protein phosphatase activity is found in cytoskeletons of y-1 mouse adrenal and bovine fasciculata cells. the activity is inhibited by three inhibitors of calmodulin (trifluoperazine, w-7 and pimozide) with ec50 in the low micromolar range. protein phosphatase activity is inhibited by vanadate, fluoride, zn2+ and pyrophosphate, stimulated by mn2+ and found to be tightly bound to the cytoskeleton. substrates for endogenous phosphatase activity were defined by one- and tw ... | 1989 | 2546840 |
| hypoglycaemia induced by antibodies to insulin receptor following a bone marrow transplantation in an immunodeficient child. | severe hypoglycaemia developed seven months after a bone marrow transplantation in a child with severe combined immunodeficiency. his serum exerted potent insulin-like activity: (a) it stimulated insulin receptor autophosphorylation and kinase activity in cell-free systems, this effect being additive to insulin; (b) it increased glucose transport in isolated soleus muscle. these insulin-like effects were due to immunoglobulins against the insulin receptor. indeed, the patient serum immunoprecipi ... | 1989 | 2546845 |
| human papillomavirus and cervical cancer. summary report. | 1989 | 2546883 | |
| functional organization and nucleotide sequence of virulence region-2 on the large virulence plasmid in shigella flexneri 2a. | the 7 kb virulence region-2 of the large (virulence) plasmid in shigella flexneri 2a encodes several proteins required for invasion of intestinal epithelial cells. insertion and deletion mutagenesis, dna subcloning and sds-polyacrylamide gel electrophoresis of proteins synthesized in minicells demonstrated five genes in this region. they encode 24, 18, 62 (ipab), 41 (ipac) and 37 (ipad)-kilodalton (kd) proteins. complementation of tn5-induced mutations in region-2 with the above plasmid construc ... | 1989 | 2552264 |
| thyrotropic activity of the ovine corticotropin-releasing factor in the chick embryo. | an injection of several doses of ovine corticotropin-releasing factor (ocrf) was made into an allantoic blood vessel of 18-day-old chick embryos. all doses used (0.5, 1, 2, and 5 micrograms) induce a quick increase of plasma corticosterone concentrations after 15 and 30 min and 1 and 2 hr and this in a dose-dependent manner with the high doses having a prolonged effect lasting up to 2 hr. an increase in plasma levels of thyroxine (t4) and triiodothyronine (t3) was observed after 1 and 2 hr. afte ... | 1989 | 2555247 |
| autotransplantation of avian parathyroid glands: an animal model for studying parathyroid function. | the parathyroid glands of chickens were autotransplanted and the return of parathyroid function following transplantation was determined. parathyroidectomy (ptx) resulted in a marked hypocalcemia (5.2 +/- 0.2 mg/dl) 4 hr following ptx. plasma calcium (pca) had declined to 4.3 +/- 0.2 mg/dl 24 hr after ptx. parathyroid glands were transplanted subcutaneously 24 hr after removal and 24 hr later, pca had risen to 8.6 +/- 0.5 mg/dl. seven days after ptx. pca increased to 10.3 +/- 0.2 mg/dl and by 14 ... | 1989 | 2555250 |
| [assessment of diagnostic prospects for a method of immunoenzyme analysis during mass testing of feces of patients for the purpose of detecting rotavirus antigens]. | 1989 | 2555970 | |
| enzyme polymorphism and clinical variability of diseases: a study of diabetes mellitus. | we investigated possible relations among four common neonatal manifestations of diabetic pregnancy (macrosomia, hypoglycemia, hypocalcemia, jaundice) and four enzyme polymorphisms (pgm1, ada, ak1, acp1 in a sample of infants born of diabetic mothers. the pattern of associations observed between the two sets of variables is consistent with known differences in enzymatic activity within phenotypes of each system, suggesting that low enzymatic activity may have unfavorable effects on fetal developm ... | 1989 | 2556341 |
| in vivo presynaptic modulation of serotonergic neurotransmission in the rat hippocampus by diazepam. | the effect of the benzodiazepine agonist, diazepam, on serotonergic (5-ht) neurotransmission was assessed in in vivo electrophysiological experiments. diazepam enhanced, in a dose-dependent manner (0.05-2 mg/kg i.v.), the effect of electrical stimulation of the ascending 5-ht pathway on the firing activity of dorsal hippocampus pyramidal neurons. this effect was blocked by the benzodiazepine antagonist, flumazenil. diazepam did not modify the efficacy of microiontophoretic application of 5-ht an ... | 1989 | 2559857 |
| [acute maternal anterior poliomyelitis in a non-endemic zone]. | the authors report the case of a 26 year old woman with acute anterior poliomyelitis contracted during the vaccination of her baby. despite having been herself vaccinated in infancy she was not protected against the poliovirus. the clinical interest of this uncommon case is a severe paralytic state with definitive paraplegia. the authors suggest serologic testing of patients born before 1967 especially if they are at risk of encountering the virus. | 1989 | 2561040 |
| characterization of l-glutamate action on the release of endogenous dopamine from the rat caudate-putamen. | in the present study the effect of l-glutamic acid (l-glu), n-methyl-d-aspartic acid (nmda), kainic acid (ka) and quisqualic acid (quis) on the release of endogenous dopamine (da) from slices of the rat caudate-putamen was investigated. da was measured by high-performance liquid chromatography coupled to an electrochemical detector. l-glu, nmda, ka and quis, in the absence of mg++, produced a dose-related, ca++-dependent increase in da release. the order of agonist efficacy was l-glu greater tha ... | 1989 | 2563769 |
| effect of endogenous opioid peptides on trh release from rat stomach in vitro. | the effect of endogenous opioid peptides (beta-endorphin, leucine-enkephalin, dynorphin 1-13 on the release of thyrotropin releasing hormone (trh) from the rat stomach in vitro was studied. the rat stomach was incubated in the medium 199 with 1.0 mg/ml of bacitracin (ph 7.4) (medium) and the amount of trh released into the medium was measured by radioimmunoassay. the release of trh from the rat stomach was inhibited significantly in a dose-related manner with the addition of endogenous opioid pe ... | 1989 | 2565806 |
| interleukin-6 in the epidermis of patients with psoriasis before and during puva treatment. | biopsies from lesional and unaffected skin of 6 patients with psoriasis, taken before and during treatment with psoralen plus uva (puva) were examined immunohistologically, using partially purified polyclonal antibodies to crude supernatants of activated human blood monocytes. by absorption with recombinant derived human monokines, we were able to demonstrate that interleukin-6 (il-6) (but not il-1 alpha or il-1 beta) was located in a laminar and granular pattern in stratum corneum, and on epide ... | 1989 | 2566221 |
| genome type analysis of adenoviruses: isolates from one year from the hannover area. | adenoviruses (av), isolated from 138 children during the year 1981 in the hannover area, were studied by dna restriction analysis with the enzymes bamhi, bglii, bsteii, ecori, hindiii, kpni, and smai and compared with the respective prototypes. varying fragment patterns were depicted and genome types analyzed. prototype-like strains of av1 and 5 were not found. types 2, 5, 1, 3, and 7 showed decreasing genetic variation in that order. altered restriction sites were physically mapped on the genom ... | 1989 | 2566302 |
| agonistic effects of anti-cd2 and anti-cd16 antibodies on human natural killer killing. | two monoclonal antibodies (moab), 9-1 (anti-cd2) and 3g8 (anti-cd16), were previously shown to enhance the cytotoxic activity of human natural killer (nk) cells. the present study examined the effect of 9-1 and 3g8 with different effector and target cells to determine whether they activate nk cells through a common mechanism. analysis of purified lymphocyte subpopulations demonstrated that the cd3+cd16+cd3- nk effector cell population is enhanced by both antibodies, while purified cd2+cd16-cd3+ ... | 1989 | 2567053 |
| long-range structure of h-ras 1-selected transgenomes. | we have used chromosome-mediated gene transfer (cmgt) and whole cell fusion to derive human-mouse hybrid cells carrying reduced human chromosomes 11, by selecting for expression of the transforming h-ras 1 oncogene. to realize the full potential of these somatic cell genetic techniques as resources for enriched dna probe isolation and the fine structure mapping of chromosomes, the nature of any molecular rearrangements that may accompany the process of dna transfer must be understood. we have an ... | 1989 | 2567060 |
| the cd2 antigen associates with the t-cell antigen receptor cd3 antigen complex on the surface of human t lymphocytes. | t lymphocytes can be activated by various stimuli directed either against the t-cell antigen receptor-cd3 antigen complex (ti-cd3) or the cd2 molecule; see ref. 1 for a review. activation signals generated by antigen binding to the antigen-specific alpha/beta heterodimer (ti) are thought to be transduced via the invariant cd3 gamma, epsilon and delta chains, and the associated zeta and eta subunits. the physiological role of the interaction of cd2 with its homologous cell-surface associated liga ... | 1989 | 2567497 |
| randomised study of myringotomy, amoxycillin/clavulanate, or both for acute otitis media in infants. | in a prospective study, 105 infants aged 3-12 months with acute otitis media were randomly assigned to one of three treatment groups: amoxycillin/clavulanate ('augmentin') alone (36 patients), myringotomy plus placebo (35 patients), or augmentin plus myringotomy (34 patients). the last two groups were double-blinded. bacterial pathogens, mainly haemophilus influenzae (of which 20% were beta-lactamase producers) and streptococcus pneumoniae, were isolated from 60% of the ear exudates and all isol ... | 1989 | 2567903 |
| assessment of pancreatic islet-cell population in the hyperglycemic athymic nude mouse: immunohistochemical, ultrastructural, and hormonal studies. | previous studies in diabetic animal models have demonstrated altered pancreatic islet-cell populations. to further characterize the diabetic syndrome in our athymic nude mouse colony, we studied the population of endocrine cells in pancreatic islets of 4-week-old normoglycemic and 8-week-old hyperglycemic athymic nude (nu/nu) mice using immunohistochemistry, morphometry, and electron microscopy. in normoglycemic 4-week athymic nu/nu mice, the proportions of b (insulin-secreting) cells and a (glu ... | 1989 | 2569196 |
| cloning and structural characterization of the genes coding for adenosylcobalamin-dependent methylmalonyl-coa mutase from propionibacterium shermanii. | the structural genes coding for both subunits of adenosylcobalamin-dependent methylmalonyl-coa mutase from the gram-positive bacterium propionibacterium shermanii have been cloned, with the use of synthetic oligonucleotides as primary hybridization probes. the genes are closely linked and are transcribed in the same direction. nucleotide sequence analysis of 4.5 kb of dna encompassing both genes allowed us to infer the complete amino acid sequence of the two subunits: the beta-subunit is the pro ... | 1989 | 2569861 |
| hydrogen peroxide mutagenicity towards salmonella typhimurium. | in bioassays conducted under controlled, comparable conditions, weak direct mutagenicity responses were observed for hydrogen peroxide in the standard (ames test) agar plate incorporation bioassay with salmonella typhimurium strains ta97, ta98, ta102, and ta1537, in a 20 min preincubation test with strains ta97, ta98, ta100, ta102, ta1537, and ta1538, and in a liquid incubation modification using strain ta1537. these results conclusively demonstrate that hydrogen peroxide is a weak mutagen, espe ... | 1989 | 2572065 |
| bacteroides loeschei pk1295 cells express two distinct adhesins simultaneously. | bacteroides loeschei synthesizes two distinct adhesins that mediate its coaggregation with streptococcus sanguis 34 and actinomyces israeli pk14. streptococcal adhesin-specific and actinomyces adhesin-specific monoclonal antibodies were used to prepare antibody-coated 5- or 10-nm gold particles. these were used in immunoelectron microscopic studies to establish that essentially all bacteroides cells in a population express both adhesins. in general, the two sizes of gold particles representing e ... | 1989 | 2572563 |
| influence of infused beta-adrenergic agonists on porcine blood metabolites and catecholamines. | anesthetized pigs were infused sequentially with increased concentrations of beta-adrenergic agonists. at selected times during infusion, blood pressure, heart rate and plasma concentrations of free fatty acids (ffa), glycerol, glucose, lactate, norepinephrine, epinephrine and dopamine were measured. azaperone, a drug used to calm the pigs before anesthesia, caused hypotension and bradycardia but did not affect plasma metabolites. infusion of norepinephrine, epinephrine, isoproterenol or clenbut ... | 1989 | 2572576 |
| restriction fragment length polymorphism of the human insulin receptor gene among mexican americans. | recently, we examined a restriction fragment length polymorphism (rflp) at the insulin receptor locus in mexican americans. this rflp can be detected using the restriction endonuclease rsa i, and has three alleles of 3.4 kb, 6.2 kb, and 6.7 kb. our data suggested that the 3.4 kilobase pair (kb) allele may be associated with type ii (non-insulin dependent) diabetes mellitus in mexican americans. we initiated studies to identify additional polymorphisms at the insulin receptor locus that might be ... | 1989 | 2573118 |
| modulation of the autonomic control of the failing heart. | the failing heart operates with an abnormal combination of heart rate, stroke volume, and enddiastolic volume. this mismatch becomes more evident during exercise of patients with heart failure, when an increase in cardiac output is achieved with higher heart rate, a lower stroke volume and a higher enddiastolic volume. using the beta 1-adrenoceptor partial agonist xamoterol which lacks beta 2-adrenoceptor agonism the response of the heart to sympathetic stimulation can be modulated. at rest and ... | 1989 | 2573339 |
| on the mechanism of positive inotropic effects of alpha-adrenoceptor agonists. | the positive inotropic effect of the alpha 1-adrenoceptor agonist phenylephrine is accompanied by an increase in the presumed second messengers inositol 1,4,5-trisphosphate (1,4,5-ip3) and inositol 1,3,4,5-tetrakisphosphate (1,3,4,5-ip4). both 1,4,5-ip3 and 1,3,4,5-ip4 sensitize myocardial contractile proteins in chemically skinned fibers. in addition to the ca++ releasing effect of 1,4,5-ip3 from the sarcoplasmic reticulum the ca++-sensitizing effect of the inositol phosphates may play a role i ... | 1989 | 2573342 |
| glutamine synthetase and glutaminase activities in various hepatoma cells. | glutamine synthetase and glutaminase activities in a series of hepatoma cells of human and rat origins were determined for comparison with normal liver tissues. marked decrease in glutamine synthetase activity was observed in the tumor cells. phosphate-dependent and phosphate-independent glutaminase activities were increased compared with those from normal liver tissues. well coupled mitochondria were isolated from huh 13 line of human hepatoma cells and human liver. oxypolarographic tests showe ... | 1989 | 2573354 |
| structure-activity relationships as a response to the pharmacological differences in beta-receptor ligands. | with a few notable exceptions, beta-receptor ligands (agonists and antagonists) belong to the aryl- or heteroaryl-ethanolamine series and to the aryl- or heteroaryl-oxypropanolamine series. structure-activity relationships for beta-adrenergic agonists show that a secondary amine in the phenylethanolamine side chain ending is essential for receptor stimulation. the 3,4-dihydroxyphenyl groups may be replaced by "phenol equivalents" (-ch2oh, -nhconh2, -choh, -nhso2ch3). in contrast, substitution at ... | 1989 | 2573372 |
| [effects of bombesin on gastrin, somatostatin, glucagon and acid secretion from isolated rat stomach in vitro]. | effects of bombesin on the secretion of gastric acid and gastro-intestinal hormones were investigated in isolated perfused rat stomach. bombesin (intra-arterial, 2 x 10(-10) mol/l, 0.3 ml/min) stimulated gastric acid secretion from basal 2.50 +/- 0.05 x 10(-1) to 8.40 +/- 1.50 x 10(-1) meq/min (p less than 0.001). exogenous addition of pentagastrin did not potentiate this effect of bombesin. bombesin induced twice releasing of gastrin and somatostatin in portal effluent but inhibited glucagon se ... | 1989 | 2574916 |
| central and peripheral actions of alpha 2-adrenergic agonists on renal function in long-evans and brattleboro rats. | the effects of 3 alpha 2-adrenergic receptor agonists on renal function in vasopressin (avp)-deficient brattleboro (di) rats were evaluated. the aim of this study was to determine the relative contribution of central versus peripheral alpha 2-adrenoceptors in mediating diuresis and natriuresis, as well as the role of alpha 2-adrenoceptors in antagonizing the actions of avp. in addition to the studies of renal function, the effects of avp deficiency on renal alpha 2-adrenoceptor affinity and numb ... | 1989 | 2575260 |
| study of the negative inotropic action of somatostatin. | somatostatin was evaluated as a chronotropic and inotropic agent in isolated, spontaneously beating guinea-pig atria. somatostatin (3 x 10(-5)-1 x 10(-3) mm) caused a concentration-dependent negative inotropic effect and a small reduction of atrial rate. the negative inotropic effect of somatostatin was evaluated at different concentrations of extracellular calcium, on the positive inotropic response induced by calcium and on the positive chronotropic response induced by adrenaline. small, nonsi ... | 1989 | 2575886 |
| immunoglobulin m, a, and g antibody response to lipopolysaccharide o antigen in symptomatic and asymptomatic shigella infections. | the antilipopolysaccharide antibody response in sera obtained from subjects involved in 10 outbreaks of shigellosis occurring in israeli military field units was determined by an enzyme-linked immunosorbent assay and a passive hemagglutination test. both tests were found to be sensitive and specific for the diagnosis of shigellosis. a significant antibody response was detected in 73 to 82% of the symptomatic and 48 to 60% of the asymptomatic subjects during the shigella sonnei and shigella flexn ... | 1989 | 2463995 |
| chronic colitis after aeromonas infection. | 1989 | 2583575 | |
| some trematodes of freshwater fishes from north vietnam with a list of recorded endohelminths by fish hosts. | the present paper comprises a systematic survey of trematodes collected from 12 species of freshwater fishes from north vietnam (the red river near hanoi). altogether 16 trematode species (14 adults and 2 juvenile forms) have been recorded. these include: amurotrema dombrowskajae, platycladorchis microacetabularis, p. macroacetabularis, neocladorchis multilobularis, carassotrema koreanum, azygia hwangtsiyui, phyllodistomum clariasi, phyllodistomum sp., orientocreadium batrachoides, masenia colla ... | 1989 | 2583614 |
| route of delivery of phenylalanine influences its effect on short-term food intake in adult male rats. | the effect of phenylalanine (phe) on plasma and brain phe and tyrosine (tyr) levels and on short-term food intake in male rats was measured after intragastric (i.g.), subcutaneous (s.c.) and intraperitoneal (i.p.) administration. compared to equimolar alanine (ala), which served as the control, phe significantly suppressed feeding at a dose of 90 mg/kg body wt when given i.p., but doses up to 720 mg/kg body wt had no effect when given i.g. or s.c. the high doses of phe given by the i.g. or s.c. ... | 1989 | 2585142 |
| expression and desensitisation of a2 purinoceptors on cultured bovine aortic endothelial cells. | purine nucleotides and their metabolites are important mediators of vascular tone. adenosine promotes relaxation of vascular smooth muscle, acting on the a2 subclass of purinoceptors. there is some evidence that these receptors are also present on vascular endothelial cells. we have therefore examined cultured aortic endothelial cells for adenosine (a2) sensitivity. bovine aortic endothelial cells (ag4762) were obtained from the institute of aging cell repository (usa), and cultured in monolayer ... | 1989 | 2590913 |
| recovery of proteins and peptides with nanogram loads on non-porous packings. | 1989 | 2592499 | |
| causes of death and the histopathologic findings in fatal shigellosis. | thirty-seven children (median age, 2 years) with shigellosis in bangladesh were subjected to postmortem examination to determine causes of death and the spectrum of intestinal histopathology. infecting species were: shigella dysenteriae 1, 7 cases; s. dysenteriae 2, 2 cases; shigella flexneri, 23 cases; shigella boydii, 4 cases; and mixed infection with shigella boydii and shigella sonnei, 1 case. complicating conditions detected before death included malnutrition in 25 cases, pneumonia in 11 ca ... | 1989 | 2594452 |
| differential blocking effects of atropine and gallamine on negative chronotropic and dromotropic responses to vagus stimulation in anesthetized dogs. | the negative dromotropic response to cervical vagus stimulation was inhibited less than the negative chronotropic response to cervical vagus stimulation by the same dose of atropine in neurally decentralized, anesthetized dogs. atropine and gallamine bind different muscarinic binding sites. we thus investigated the blocking effects of atropine and gallamine on the negative chronotropic and dromotropic responses to stimulation of the vagus nerves. we stimulated the intracardiac vagus nerves to th ... | 1989 | 2600814 |
| rheumatoid synovial fluid reconstitutes the b-cell defect in cba/n mice. | synovial fluid from patients with rheumatoid arthritis (ra-sf) contains a biological activity which can replace t cells for activation of antibody secretion in human blood lymphoid cells and which can also induce the selective differentiation of igg2b-secreting cells in lipopolysaccharide (lps)-pre-activated mouse spleen cells. the b-cell activity of this factor was studied in cba/n mice which have an x-linked b-cell immunodeficiency which manifests itself as a defective humoral response to cert ... | 1989 | 2602914 |
| cardiopulmonary baroreflex control of brachial artery diameter in sustained essential hypertension. | an exaggerated reflex response of forearm resistance vessels following stimulation of cardiac mechanoreceptors has been observed in human hypertension. in the present study we analyze the possibility that such an increased response also involves the large brachial artery. for that purpose, the influence of leg elevation in subjects in a supine position on brachial artery diameter and blood flow velocity was studied in 12 healthy, normal males and 17 males of the same age with sustained essential ... | 1989 | 2607141 |
| signal structure of the cis-acting element recognized by virg protein, a positive regulator in agrobacterium. | virg protein is known to be the positive regulator for vir genes of ri and ti plasmids in agrobacterium. to investigate the cis-acting element recognized by virg, we have determined the transcriptional start points of the vir genes on ri plasmid (pria4b). from the analysis of the upstream sequences, it has been found that the sequences generally similar to 5'tg(a/t)aa(c/t)3' appear in phase with an 11-base pair interval, and that the -35 and -10 regions of the promoters are located nearly in the ... | 1989 | 2608465 |
| symptoms associated with diarrhoeal illness at san lazaro hospital, manila in 1983 and 1984. | the clinical features associated with various agents of diarrhoeal disease were studied using 2,836 patients admitted to san lazaro hospital, manila. three general patient groups were considered including single pathogen isolations, "multiple pathogen" isolations, and "no pathogen" isolations. in general, symptoms of diarrhoeal illness were found to be non-specific. however, shigella flexneri. vibrio parahemolyticus, and rotavirus were significantly associated with a number of prominent symptoms ... | 1989 | 2609210 |
| three dimensional observations of the palisade-shaped nerve endings of normal hair of rat's snout. | the nerve endings of normal hair of the rat's snout, partially digested with trypsin and hydrochloric acid, were studied by scanning electron microscopy. each lanceolate structure measured ca. 10 microns in length and was arranged around the hair follicle. these palisade-shaped nerve endings were situated almost beneath the sebaceous glands, ran upward, parallel to the axis of the hair follicle, and terminated in pointed shape. 2 kinds of cells, teloglia cell type i showing flat profile, and tel ... | 1989 | 2610376 |
| studies on acute toxic effects to keratinocytes induced by hematoporphyrin derivatives and laser light. | human epidermal keratinocytes were grown in culture and the uptake of hematoporphyrin derivatives (hpds) used in photodynamic therapy was estimated. keratinocytes loaded with hpds were irradiated with laser light of 632 nm generated by a helium-neon laser and cell toxicity was determined by the trypan blue exclusion test and the measurement of enzyme release. with increasing intracellular concentration of hpds and with increasing intensity of the laser light, an increasing number of cells took u ... | 1989 | 2610520 |
| ecotoxicological characterization of industrial wastewater--sulfite pulp mill with bleaching. | different process wastewaters from a sulfite pulp mill with bleaching were characterized by chemical analysis and toxicity tests. the amount of adsorbable organically bound halogen (aox) from the bleachery was 3.6 kg per ton pulp. the extractable organically bound chlorine was 15% of aox. some identified organochlorine compounds in the effluent could be traced in the receiving water. effluents from the chlorination and alkaline extraction stages and the condensate were the main contributors to t ... | 1989 | 2612424 |