Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| sindbis virus replicon particles encoding calreticulin linked to a tumor antigen generate long-term tumor-specific immunity. | alphavirus vectors have emerged as a promising strategy for the development of cancer vaccines and gene therapy applications. in this study, we used the replication-defective vaccine vector sin replicon particles from a new packaging cell line (pcl) to develop sin replicon particles encoding calreticulin (crt) linked to a model tumor antigen, human papillomavirus type 16 (hpv16) e7 protein. the linkage of crt to e7 in sin replicon particles resulted in a significant increase in e7-specific cd8(+ ... | 2006 | 16645621 |
| time to clearance of human papillomavirus infection by type and human immunodeficiency virus serostatus. | persistent infection with high-risk human papillomavirus (hpv) is central to cervical carcinogenesis. certain high-risk types, such as hpv16, may be more persistent than other hpv types, and type-specific hpv persistence may differ by hiv serostatus. this study evaluated the association between hpv type and clearance of hpv infections in 522 hiv-seropositive and 279 hiv-seronegative participants in the hiv epidemiology research study (hers, united states, 1993-2000). type-specific hpv infections ... | 2006 | 16646070 |
| vulvar, vaginal, and perianal intraepithelial neoplasia in women with or at risk for human immunodeficiency virus. | to compare the incidence of vulvar, vaginal, and perianal intraepithelial neoplasia among human immunodeficiency virus (hiv)-infected women with a group of well-matched high-risk hiv-uninfected controls. | 2006 | 16648406 |
| nuclear import strategies of high-risk hpv18 l2 minor capsid protein. | we have investigated the nuclear import strategies of high-risk hpv18 l2 minor capsid protein. hpv18 l2 interacts with kap alpha2 adapter, and kap beta2 and kap beta3 nuclear import receptors. moreover, binding of rangtp to either kap beta2 or kap beta3 inhibits their interaction with l2, suggesting that these kap beta/l2 complexes are import competent. mapping studies show that hpv18 l2 contains two nlss: in the n-terminus (nnls) and in the c-terminus (cnls), both of which can independently med ... | 2006 | 16733063 |
| [the development and clinical application of papillomavirus genotyping by dna chip]. | to develop a new platform for genotyping human papillomavirus (hpv) and to investigate its effect in clinical application. | 2006 | 16737573 |
| hpv16 dna in histologically confirmed tumour-free neck lymph nodes of head and neck cancers. | human papillomavirus (hpv) has been demonstrated in lymph node neck metastases (nm) of hpv-positive squamous cell carcinomas of the head and neck (hnscc), underscoring the possible role of hpv for hnscc progression. reports on hpv infections in histopathologically tumour-free lymph-nodes of the scc of the uterine cervix developing higher rates of lymph-node metastases and recurrences later in the survey of the patients was the starting point of the present study. | 2006 | 16739336 |
| epidemiological and functional implications of molecular variants of human papillomavirus. | human papillomavirus genomes are classified into molecular variants when they present more than 98% of similarity to the prototype sequence within the l1 gene. comparative nucleotide sequence analyses of these viruses have elucidated some features of their phylogenetic relationship. in addition, human papillomavirus intratype variability has also been used as an important tool in epidemiological studies of viral transmission, persistence and progression to clinically relevant cervical lesions. u ... | 2006 | 16751975 |
| enhanced detection and typing of human papillomavirus (hpv) dna in anogenital samples with pgmy primers and the linear array hpv genotyping test. | the roche pgmy primer-based research prototype line blot assay (pgmy-lb) is a convenient tool in epidemiological studies for the detection and typing of human papillomavirus (hpv) dna. this assay has been optimized and is being commercialized as the linear array hpv genotyping test (la-hpv). we assessed the agreement between la-hpv and pgmy-lb for detection and typing of 37 hpv genotypes in 528 anogenital samples (236 anal, 146 physician-collected cervical, and 146 self-collected cervicovaginal ... | 2006 | 16757590 |
| development of a sensitive and specific assay combining multiplex pcr and dna microarray primer extension to detect high-risk mucosal human papillomavirus types. | the importance of assays for the detection and typing of human papillomaviruses (hpvs) in clinical and epidemiological studies has been well demonstrated. several accurate methods for hpv detection and typing have been developed. however, comparative studies showed that several assays have different sensitivities for the detection of specific hpv types, particularly in the case of multiple infections. here, we describe a novel one-shot method for the detection and typing of 19 mucosal high-risk ... | 2006 | 16757593 |
| human papillomavirus testing and liquid-based cytology: results at recruitment from the new technologies for cervical cancer randomized controlled trial. | although testing for human papillomavirus (hpv) has higher sensitivity and lower specificity than cytology alone for detecting cervical intraepithelial neoplasia (cin), studies comparing conventional and liquid-based cytology have had conflicting results. | 2006 | 16757701 |
| hpv testing in routine cervical screening: cross sectional data from the artistic trial. | to evaluate the effectiveness of human papillomavirus (hpv) testing in primary cervical screening. this was a cross-sectional study from the recruitment phase of a prospective randomised trial. women were screened for hpv in addition to routine cervical cytology testing. greater manchester, attendees at routine nhs cervical screening programme. in all, 24 510 women aged 20-64 screened with liquid-based cytology (lbc) and hpv testing at entry. hpv testing in primary cervical screening. type-speci ... | 2006 | 16773068 |
| distribution of human papillomavirus types in thinprep papanicolaou tests classified according to the bethesda 2001 terminology and correlations with patient age and biopsy outcomes. | 2006 | 16773631 | |
| sexually transmitted human papillomavirus type variations resulting in high grade cervical dysplasia in north-east north dakota and north-west minnesota. | a review of pap smear diagnoses from a reference laboratory in grand forks, north dakota over a 3-year period (07/00 to 10/03) revealed a two-fold higher rate of high grade squamous intraepithelial lesion in a community in northwest minnesota (roseau, 0.486%) than in northeast north dakota (grand forks, 0.249%), in spite of both having similar rates of low-grade squamous intraepithelial lesion (1.33% vs.1.30% respectively) | 2006 | 16776841 |
| pediatric anogenital warts: a 7-year review of children referred to a tertiary-care hospital in montreal, canada. | the objectives of this study were to delineate the clinical characteristics of a hospital-referred pediatric population infected with anogenital warts and to investigate the possible relationships between human papillomavirus types and the identified clinical characteristics. over a 7-year period, 72 patients under the age of 12 years were seen at our dermatology clinic for anogenital warts, corresponding to a prevalence of 1.7/1000 in our patient population. sixty-four percent (46/72) were girl ... | 2006 | 16780463 |
| presence of high-risk human papillomavirus sequences in breast cancer tissues and association with histopathological characteristics. | to examine the presence of human papillomavirus (hpv) in breast cancer tissues. | 2006 | 16780823 |
| covariates of cervical cytokine mrna expression by real-time pcr in adolescents and young women: effects of chlamydia trachomatis infection, hormonal contraception, and smoking. | measurements of mucosal immune parameters in the uterine cervix are potentially influenced by numerous factors, including infections, endogenous and exogenous hormones, semen, and nicotine and its metabolites in cervical mucus. the objective of this study was to examine correlates of immunoregulatory cytokine mrna expression in cervical cytology samples in a cross-sectional design. samples, collected at study entry by cervical cytology brush from 368 women aged 13-21 enrolled in a longitudinal s ... | 2006 | 16783462 |
| [cervical infection epidemiology of human papillomavirus in ushuaia, argentina]. | genital infection with human papillomavirus (hpv) is decisive in the causation of cervical cancer. in order to evaluate the epidemiology of hpv infection in ushuaia, province of tierra del fuego, argentina, 132 endocervical cytobrushes from preneoplastic and neoplastic cases and controls were studied. detection and typing of the viral genome was performed by polymerase chain reaction, combined with a restriction fragment length polymorphism assay or hybridization. the overall prevalence of hpv i ... | 2006 | 16784128 |
| vaccines against rotavirus and human papillomavirus (hpv). | to briefly review strategies aimed at the development of rotavirus and hpv vaccines, with emphasis on the current status of studies assessing the safety, reactogenicity, immunogenicity and efficacy of recently developed vaccines. | 2006 | 16786101 |
| detection of ebv and hpv in nasopharyngeal carcinoma by in situ hybridization. | nasopharyngeal carcinoma (npc) is a common cancer in southeast asia and is frequently associated with epstein-barr virus (ebv). human papilloma virus (hpv) is an epitheliotrophic oncogenic virus that has been detected in a variety of head and neck tumors including npc. this retrospective study was undertaken to investigate the prevalence of ebv and hpv infection subtypes 6/11 and 16/18 in 20 patients with npc. | 2006 | 16787643 |
| determination of hpv type 16 and 18 viral load in cervical smears of women referred to colposcopy. | it has been recognized that human papillomavirus infection is the major causal factor for high-grade cervical lesions. the aim of the study was to evaluate the relationship between hpv 16 and 18 viral loads and cervical status in different age strata. a duplex real time pcr method was devised to determine hpv 16 and 18 viral load per million of human cells using an in house plasmidic construct as a standard of quantification. the 151 cervical scrapes were collected before colposcopic examination ... | 2006 | 16789021 |
| papillomavirus virus-like particles as vehicles for the delivery of epitopes or genes. | papillomaviruses (pvs) are simple double-strand dna viruses whose virion shells are t = 7 icosahedrons and composed of major capsid protein l1 and minor capsid protein l2.l1 alone or together with l2 can self-assemble into virus-like particles (vlps) when expressed in eukaryotic or prokaryotic expression systems. although the vlps lack the virus genome dna, their morphological and immunological characteristics are very similar to those of nature papillomaviruses. pv vlp vaccination can induce hi ... | 2006 | 16791442 |
| finding a vaccine for human papillomavirus. | 2006 | 16798383 | |
| the human papillomavirus type 16 e7 protein binds human interferon regulatory factor-9 via a novel pest domain required for transformation. | it is critical that viruses are able to avoid the antiviral activities of interferon (ifn). we have shown previously that the human papillomavirus (hpv) is able to avoid ifn-alpha via interaction of the hpv-16 e7 protein with ifn regulatory factor-9 (irf-9). here, we investigated the details of the interaction using hpv-16 e7 peptide mapping to show that irf-9 binds hpv-16 e7 in a domain encompassing amino acids 25-36. a closer examination of this region indicates this is a novel proline, glutam ... | 2006 | 16800784 |
| human papillomavirus genotype prevalence in cervical biopsies from women diagnosed with cervical intraepithelial neoplasia or cervical cancer in melbourne, australia. | multicenter international phase iii clinical trials using multivalent human papillomavirus (hpv) vaccines for cervical cancer (cc) prevention are underway. as hpv immunity is type specific, defining hpv genotype prevalence in different regions to ascertain whether predominant types differ geographically is considerably important prior to vaccine implementation. this study aimed to define hpv genotypes present in cc and high-grade dysplasia among women in melbourne, australia. hpv genotype analys ... | 2006 | 16803479 |
| prevalence of human papillomavirus types 16 and 18 in cervical adenocarcinoma and its precursors in scottish patients. | our aim was to determine the prevalence of human papillomavirus (hpv) types 16 and 18 in cervical adenocarcinoma (and its precursors) in scottish patients. nucleic acid was extracted from paraffin-embedded, formalin-fixed tissues. we examined 119 cases of invasive adenocarcinoma, 20 cases of adenocarcinoma in situ, and 16 cases of normal glandular epithelium. hpv dna was detected by polymerase chain reaction using type-specific primers for the e6 and e7 genes of hpv-16 and hpv-18 with conformati ... | 2006 | 16803480 |
| prediction of high-grade cervical disease with human papillomavirus detection in women with glandular and squamous cytologic abnormalities. | the objective of this study was to assess whether human papillomavirus (hpv) detection with hybrid capture ii (hc ii) can help predict the presence and the nature, glandular or squamous, of histologic cervical lesions in women referred due to atypical glandular cells (agc) or high-grade squamous intraepithelial lesion (hsil). a total of 247 women were included. referral pap smears comprised agc (51 cases), agc plus hsil (28 cases), adenocarcinoma in situ (10 cases), and hsil (158 cases). all pat ... | 2006 | 16803485 |
| prime-boost vaccination strategy in women with high-grade, noncervical anogenital intraepithelial neoplasia: clinical results from a multicenter phase ii trial. | the objective of this study was to determine the clinical effectiveness of a prime-boost human papillomavirus (hpv) vaccine regimen. a nonrandomized phase ii prime-boost vaccine trial was conducted. women with biopsy-proven anogenital intraepithelial neoplasia (agin) 3 were vaccinated with three doses of a recombinant fusion protein comprising hpv 16, e6/e7/l2 (ta-cin) followed by one dose of a recombinant vaccinia virus encoding hpv 16 and 18 e6/e7 (ta-hpv). clinical responses were evaluated by ... | 2006 | 16803488 |
| a subgroup of hox abd-b gene is differentially expressed in cervical cancer. | the hox genes are a family of transcription factors that bind to specific sequences of dna in target genes regulating their expression. the role of hox genes in adult cell differentiation is still obscure, but growing evidence suggests that they may play an important role in the development of cancer. in order to study the role of the hox abd-b genes in cervical cancer, we analyzed their expression in cervical tissues. reverse transcription-polymerase chain reaction and rna in situ hybridization ... | 2006 | 16803519 |
| human papillomavirus associated with papillary squamous cell carcinoma of the oropharynx in a renal transplant recipient. | the papillary squamous cell carcinoma (pscc) is a rare variant of the head and neck squamous cell carcinoma. established etiological factors can include tobacco smoking and heavy alcohol abuse. moreover, human papillomavirus infection can be involved in the pathogenesis of pscc. this tumor is more frequent in patients with immunosuppression including those who have received a transplant. most of the cases are produced by genotype hpv-6 and hpv-16, although there is a possibility of infection by ... | 2006 | 16804664 |
| deregulation of eif4e: 4e-bp1 in differentiated human papillomavirus-containing cells leads to high levels of expression of the e7 oncoprotein. | infections with high-risk human papillomaviruses (hpvs) are linked to more than 95% of cervical cancers. hpvs replicate exclusively in differentiated cells and the function of the hpv e7 oncoprotein is essential for viral replication. in this study, we investigated the mechanism that regulates e7 expression in differentiated cells. the level of e7 protein was strongly induced in hpv-containing caski, hok-16b, and bap-t cells during growth in methylcellulose-containing medium, a condition that in ... | 2006 | 16809313 |
| p16-positive small cell neuroendocrine carcinoma of the endometrium. | we report herein a small cell neuroendocrine carcinoma of the endometrium that extended to the cervix and showed strong immunohistochemical staining for p16. the p16 staining raised the possibility of a human papillomavirus (hpv)-related tumor, because in the cervix, a positive p16 immunohistochemical stain is presumptive evidence of hpv. however, the current case was hpv negative. we discuss the molecular pathogenesis of non-hpv-related increased p16 expression. | 2006 | 16810063 |
| [loss of heterozygosity at chromosome 6 as a marker of early genetic alterations in cervical intraepithelial neoplasias and microinvasive carcinomas]. | oncogenic human papilloma viruses (mostly hpv types 16 and 18) are the major cause of cervical intraepithelial neoplasia (cin) that progress into cervical cancer (cc). to reveal early genetic alterations at chromosome 6 important for cc progression we have analyzed loss of heterozygosity (loh) in dna from 45 cin cases, 47 microcarcinomas and 19 invasive squamous cell carcinomas stage ib. loh analysis of dna samples prepared with microdissection from all cin foci as well as from cc lesions and sy ... | 2006 | 16813163 |
| gene expression profiles reveal an upregulation of e2f and downregulation of interferon targets by hpv18 but no changes between keratinocytes with integrated or episomal viral genomes. | persistent infections with human papillomaviruses type 18 can result in the development of cervical cancer. hpv18 genomes persist extrachromosomally in low-grade and precancerous lesions but are always integrated in cervical cancers, and this might contribute to the progression of hpv18-induced lesions. to address whether integration induces additional changes in host cells, several keratinocyte lines with wild type and replication-deficient e1 mutant hpv18 (e1c-ttl) genomes were analyzed with h ... | 2006 | 16814354 |
| high concordance of results of testing for human papillomavirus in cervicovaginal samples collected by two methods, with comparison of a novel self-sampling device to a conventional endocervical brush. | a user-friendly self-sampling method for collecting representative cervical cell material would lower the threshold for women to respond to the invitation for cervical screening. in the present article, we introduce such a device; we have evaluated its sensitivity and specificity to detect high-grade cervical intraepithelial neoplasia (cin), via high-risk human papillomavirus (hrhpv) detection and liquid-based cytology (lbc), compared to endocervical brush samples obtained by gynecologists. wome ... | 2006 | 16825374 |
| development of a topical protein therapeutic for human papillomavirus and associated cancers. | human papillomaviruses (hpvs) are the causative agents of several disease states, including genital warts and cervical cancer. there are around 500 million cases of genital warts per annum worldwide and around 450,000 cases of cervical cancer. although hpv vaccines should eventually reduce the incidence of these diseases, new and effective treatments are still urgently required. the e2 (early) proteins from some hpv types induce growth arrest and apoptosis, and these proteins could be used as th ... | 2006 | 16831020 |
| human papillomavirus genotype distribution and cervical squamous intraepithelial lesions among high-risk women with and without hiv-1 infection in burkina faso. | human papillomavirus (hpv) infection and cervical squamous intraepithelial lesions (sils) were studied in 379 high-risk women. human papillomavirus dna was detected in 238 of 360 (66.1%) of the beta-globin-positive cervical samples, and 467 hpv isolates belonging to 35 types were identified. multiple (2-7 types) hpv infections were observed in 52.9% of hpv-infected women. the most prevalent hpv types were hpv-52 (14.7%), hpv-35 (9.4%), hpv-58 (9.4%), hpv-51 (8.6%), hpv-16 (7.8%), hpv-31 (7.5%), ... | 2006 | 16832413 |
| biomolecular study of the correlation between papillomatosis of the vulvar vestibule in adolescents and human papillomavirus. | the main goal of this study was to investigate, through a biomolecular study, the correlation between papillomatosis of the vulvar vestibule and human papillomavirus (hpv) infection, as well as to establish the necessity of treatment. a total of 44 female adolescents between 12 and 18 years of age were selected through a prospective study with a confirmed diagnosis of papillomatosis of the vulvar vestibule. vulvar biopsies were obtained for the histological and biomolecular detection of hpv dna ... | 2006 | 16832565 |
| role for wee1 in inhibition of g2-to-m transition through the cooperation of distinct human papillomavirus type 1 e4 proteins. | the infectious cycle of human papillomavirus type 1 (hpv1) is accompanied by abundant expression of the full-length e1;e4 protein (17-kda) and smaller e4 polypeptides (16-, 11-, and 10-kda) that arise by sequential loss of n-terminal e1;e4 sequences. hpv1 e4 inhibits g(2)-to-m transition of the cell cycle. here, we show that hpv1 e4 proteins mediate inhibition of cell division by more than one mechanism. cells arrested by coexpression of e1;e4 (e4-17k) and a truncated protein equivalent to the 1 ... | 2006 | 16840322 |
| relationship between cyclin g1 and human papilloma virus infection in cervical intraepithelial neoplasia and cervical carcinoma. | to evaluate the overexpression of cyclin g1 in cervical intraepithelial neoplasia (cin) and cervical carcinoma, and the correlation between cyclin g1 and high-risk human papilloma virus (hpv) infection. | 2006 | 16845792 |
| comparison between immunofluorescence and pcr in detecting human papilloma virus in condyloma acuminata. | to compare the effectiveness of immunofluorescence and polymerase chain reaction (pcr) in detecting human papilloma virus (hpv) in condyloma acuminata (ca). | 2006 | 16845802 |
| frequent gain of the human telomerase gene terc at 3q26 in cervical adenocarcinomas. | the level of genomic amplification of the human telomerase gene terc, which maps to chromosome band 3q26, was determined in primary cervical adenocarcinomas. interphase nuclei prepared from archival material of 12 primary cervical adenocarcinomas, eight of which were human papillomavirus positive, were hybridised with a triple colour probe set specific for centromeres of chromosomes 3 and 7 and the terc gene. we observed high proportions of nuclei with increased absolute copy numbers for terc in ... | 2006 | 16847471 |
| human papillomavirus vaccines. | to review the pharmacology, efficacy, safety, tolerability, and pharmacoeconomics of cervarix and gardasil, 2 human papillomavirus (hpv) vaccines. | 2006 | 16849621 |
| upregulation of nuclear factor-kappab (nf-kappab) is related to the grade of cervical intraepithelial neoplasia, but is not an independent predictor of high-risk human papillomavirus or disease outcome in cervical cancer. | nuclear factor-kappab (nf-kappab) has a pivotal function in controlling a wide variety of gene functions, and has shown to be constitutively activated in many human cancers. the molecular links of nf-kappab to oncogenic human papillomavirus (hpv) in cervical intraepithelial neoplasia (cin) lesions and its prognostic value in cervical cancer (cc) are incompletely understood. as part of our hpv-pathogeniss study, a series of 150 squamous-cell carcinomas (sccs) and 152 cin lesions were examined usi ... | 2006 | 16850495 |
| human papillomavirus 6a lesions of the lower eyelid and genitalia. | a 42-year-old white man presented with a lower eyelid lesion and recurrent condylomata acuminata of his genitalia due to human papillomavirus (hpv) type 6a. polymerase chain reaction analysis of both lesions was performed. the eyelid lesion was excised and found to be a papilloma caused by hpv6a infection. an extensive literature search revealed one previous report of concurrent conjunctival papilloma and genital papilloma involving hpv6a. rare cases of solitary lesions of the eyelid secondary t ... | 2006 | 16855513 |
| the impact of the use of col-1492, a nonoxynol-9 vaginal gel, on the presence of cervical human papillomavirus in female sex workers. | this study investigated the effectiveness of a nonoxynol-9 (52.5mg, 3.5%), vaginal gel (advantage s), in the prevention of human papillomavirus (hpv) infection in female sex workers. we showed by hpv dna determination in cervico-vaginal rinses a significant increase in multiple (>1) hpv infection in hiv-1 seropositive women compared with hiv-1 seronegative women (or 4.0, 95% ci 1.3-11.9). we also demonstrated a significant increase in multiple hpv infections in hiv-1 seronegative women using non ... | 2006 | 16860426 |
| cellular immune responses to hpv-18, -31, and -53 in healthy volunteers immunized with recombinant hpv-16 l1 virus-like particles. | human papillomavirus-like particles (hpv vlp) are candidate vaccines that have shown to be efficacious in reducing infection and inducing robust antiviral immunity. neutralizing antibodies generated by vaccination are largely type-specific, but little is known about the type-specificity of cellular immune responses to vlp vaccination. to determine whether vaccination with hpv-16 l1vlp induces cellular immunity to heterologous hpv types (hpv-18, hpv-31, and hpv-53), we examined proliferative and ... | 2006 | 16863657 |
| expression of e6/e7 mrna from 'high risk' human papillomavirus in relation to cin grade, viral load and p16ink4a. | detection of e6/e7 mrna expression with real-time nucleic acid sequence-based amplification assay (nasba) method (pretect hpv-proofer) from high-risk types of human papillomaviruses (hr-hpv) were compared with the presence of viral load, determined with quantitative real-time pcr in 80 cervical samples. results regarding positivity and typing were in agreement using the two methods. however, there was no correlation between viral loads for hpv 16 or 18/45 and oncogene expression. among 15 women ... | 2006 | 16865288 |
| human papilloma virus dna exposure and embryo survival is stage-specific. | human papillomavirus (hpv) has been shown to disrupt late-stage implanting embryos. the objectives were (a) to assess the development of early embryos exposed to hpv dna and (b) to analyze the blastocyst hatching process after hpv exposure. | 2006 | 16871451 |
| deficiencies in myeloid antigen-presenting cells in women with cervical squamous intraepithelial lesions. | there is little information on the function of dendritic cells in women with human papillomavirus (hpv)-related cervical squamous intraepithelial lesions (sils). in the current study the functions of dendritic cells in the development of t-cell immunity in women with cervical sils were assessed. | 2006 | 16874820 |
| prevalence of high risk human papillomavirus types among nicaraguan women with histological proved pre-neoplastic and neoplastic lesions of the cervix. | to determine the prevalence of high risk human papillomavirus (hpv) types in nicaraguan women with histological proved pre-neoplastic and neoplastic cervical lesions, and to assess its potential impact on preventive strategies. | 2006 | 16877588 |
| reverse transcription-polymerase chain reaction and western blotting analysis for detection of p63 isoforms in uterine cervical cancers. | the aim of this study was to investigate the role of tap63 and deltanp63 isoforms in uterine cervical cancers. the messenger rna (mrna) and protein expressions of ta and deltan forms as well as alpha, beta, and gamma isoforms of p63 were studied in seven siha, me-180, snu17, snu902, snu1160, snu703, and snu1299 human papillomavirus (hpv)-positive uterine cervical squamous cell carcinoma (scc) cell lines, one ht3 hpv-negative scc cell line, and one hela adenocarcinoma cell line using reverse tran ... | 2006 | 16884378 |
| prevention of recurrent respiratory papillomatosis: role of hpv vaccination. | recurrent respiratory papillomatosis is a rare, but devastating, cause of airway lesions in children and adults. this disease is caused by human papilloma virus subtypes 6 and 11. at this time there are two vaccines in late stages of development seeking food and drug administration (fda) approval to prevent cervical cancer, which is also caused by human papilloma virus. one of these vaccines has been developed to stimulate immunity to the most common subtypes that cause cervical cancer but also ... | 2006 | 16884786 |
| follow-up of high-grade squamous intra-epithelial lesions (h-sils) in human immunodeficiency virus (hiv)-positive and human papillomavirus (hpv)-positive women. analysis of risk factors. | human immunodeficiency virus (hiv)-positive women are at high risk of co-infection from human papillomavirus (hpv) and of developing squamous intraepithelial lesions of the cervix. | 2006 | 16886651 |
| p14arf, a prognostic predictor in hpv-negative vulvar carcinoma. | the present study addressed the impact of human papillomavirus (hpv), p14, and the product of the retinoblastoma gene (prb) in vulvar carcinoma in relation to other clinicopathologic variables and prognosis. we immunohistochemically studied 217 primary tumors from patients with vulvar carcinoma for the expression of prb and p14. by the use of in situ hybridization, the primary tumors and 7 lymph node metastases were studied for the presence of hpv-16, hpv-18, hpv-31, and hpv-33 dna. hpv-infected ... | 2006 | 16891203 |
| the occurrence of genital types of human papillomavirus in normal pregnancy and in pregnant renal transplant recipients. | the aim of this study was to evaluate: 1) the prevalence of human papillomavirus (types 6 and 11 carrying a low risk of neoplasia, and type 16 implicated as cause of cervical neoplasia and cancer) in normal pregnant women and pregnant renal transplant recipients. 2) the correlation between maternal hpv infection and hpv presence in the cord blood and the oral cavity of the neonate. evaluation of a likely, additional route of hpv transmission to the fetus, apart from the infected birth canal duri ... | 2006 | 16891989 |
| gateways to clinical trials. | gateways to clinical trials are a guide to the most recent clinical trials in current literature and congresses. the data in the following tables have been retrieved from the clinical trials knowledge area of prous science integrity, the drug discovery and development portal, http://integrity.prous.com. this issue focuses on the following selection of drugs:(r)-flurbiprofen, 90yttrium-dota-huj591; abt-510, acp-103, ad5-fgf4, adalimumab, ademetionine, ag-7352, alemtuzumab, amb a 1 iss-dna, anakin ... | 2006 | 16894408 |
| analyses of variant human papillomavirus type-16 e5 proteins for their ability to induce mitogenesis of murine fibroblasts. | human papillomavirus type 16 (hpv-16) e5 protein co-operates with epidermal growth factor to stimulate mitogenesis of murine fibroblasts. currently, little is known about which viral amino acids are involved in this process. using sequence variants of hpv-16 e5 we have investigated their effects upon e5 transcription, cell-cycling and cell-growth of murine fibroblasts. | 2006 | 16899131 |
| [clinical evaluation of high-risk hpv detection by hybrid capture ii in screening cervical intraepithelial neoplasma]. | to evaluate the value of high-risk hpv (hrhpv) detection by hybrid capture ii (hc2) in screening cervical intraepithelial neoplasm (cin). | 2006 | 16900642 |
| cpg methylation of hpv 16 lcr at e2 binding site proximal to p97 is associated with cervical cancer in presence of intact e2. | human papillomavirus type 16 (hpv-16) e2 protein negatively regulates transcription of the e6 and e7 genes. this study was done to test the hypothesis that methylation of the hpv 16 long control region (lcr) is overrepresented among cervical cancer (cacx) cases compared to cytologically normal controls harboring intact e2 gene. methylation of the e2 binding site (e2bs-i), proximal to the p97 promoter, was assessed by hpaii/ mspi restriction digestion while mcrbc digestion was used to assess lcr- ... | 2006 | 16905170 |
| effect of replens gel used with a diaphragm on tests for human papillomavirus and other lower genital tract infections. | little is known about effects of vaginal lubricants with barrier contraceptives on detection of sexually transmissible infections. we hypothesized that replens gel used with a diaphragm would neither inhibit human papillomavirus (hpv) detection in cervical samples and chlamydia (ct) and gonorrhea (gc) detection in urine samples, nor affect cervical cytology quality. | 2006 | 17012985 |
| early detection of cervical cancer by human papillomavirus dna testing: case reports. | 2006 | 17012993 | |
| [cervical cancer screening of hiv-positive women: is a prolongation of the screening interval meaningful?]. | cervical cancer screening guidelines stated recently that the screening interval of healthy women can be extended up to 3 years. can those recommendations be applied for high risk populations? | 2006 | 17001558 |
| simultaneous amplification and identification of 25 human papillomavirus types with templex technology. | the majority of existing human papillomavirus (hpv) genotyping assays are based on multiplex pcr using consensus or degenerate primers. we developed a templex hpv assay that simultaneously detects and identifies 25 common hpv genotypes in a single-tube reaction using type-specific primers for the hpv-specific e6 and e7 genes. the analytical sensitivities of the templex assay for hpv type 16 (hpv-16), -18, and -56 were 20, 100, and 20 copies per reaction mixture, respectively. the templex assay p ... | 2006 | 17005760 |
| regulation of topbp1 oligomerization by akt/pkb for cell survival. | regulation of e2f1-mediated apoptosis is essential for proper cellular growth. this control requires topbp1, a brct (brca1 carboxyl-terminal) domain-containing protein, which interacts with e2f1 but not other e2fs and represses its proapoptotic activity. we now show that the regulation of e2f1 by topbp1 involves the phosphoinositide 3-kinase (pi3k)-akt signaling pathway, and is independent of pocket proteins. akt phosphorylates topbp1 in vitro and in vivo. phosphorylation by akt induces oligomer ... | 2006 | 17006541 |
| the human papillomavirus vaccines. | genital human papillomavirus (hpv) infection is the most common sexually transmitted infection (sti) among sexually active couples. its annual incidence is approximately 5.5 million. overall, an estimated 75% of sexually active men and women have been exposed to hpv at some point in their lives. hpv-16 and -18 account for about 70% of cancers of the cervix, vagina and anus, and for about 30%-40% of cancers of the vulva, penis and orophaynx. cancer of the cervix uteri is the second most cancer am ... | 2006 | 17010274 |
| relationship of pregnancy to human papillomavirus among human immunodeficiency virus-infected women. | because parity is a reported risk factor for cervical cancer, we sought to estimate the effects of pregnancy on the prevalence, incident detection, and copy number of human papillomavirus (hpv) among human immunodeficiency virus (hiv)-infected women, patients at high risk for cervical cancer. | 2006 | 17012459 |
| [a case-control study on the association between folate and cervical cancer]. | to evaluate the possible effects of folate on cervical carcinogenesis and the interaction of folate and human papillomaviruses 16 (hpv16). | 2006 | 16981341 |
| human papillomavirus (hpv) vaccine: a position statement of the society for adolescent medicine. | 2006 | 16982407 | |
| how will hpv vaccines affect cervical cancer? | cancer of the uterine cervix is the second largest cause of cancer deaths in women, and its toll is greatest in populations that lack screening programmes to detect precursor lesions. persistent infection with 'high risk' genotypes of human papillomavirus (hpv) is necessary, although not sufficient, to cause cervical carcinoma. therefore, hpv vaccination provides an opportunity to profoundly affect cervical cancer incidence worldwide. a recently licensed hpv subunit vaccine protects women from a ... | 2006 | 16990853 |
| human papilloma virus and p53 expression in bladder cancer in egypt: relationship to schistosomiasis and clinicopathologic factors. | the aim of the current study was to compare the role of p53 and human papillomavirus (hpv) in schistosomiasis-related and schistosomiasis-unrelated carcinoma of the urinary bladder. to achieve this aim, we investigated 114 bladder carcinomas for p53 oncoprotein expression by immunohistochemistry and for human papillomavirus by in situ hybridization technique. the results revealed that 64 tumors (56.1%) were schistosomiasis-associated. sixty seven (58.8%) were transitional cell carcinomas and 32 ... | 2006 | 16998598 |
| histological features of human papilloma virus 16 and its association with the development and progression of anal squamous cell carcinoma. | to investigate the development of anal squamous cell carcinoma (scc) and the expression patterns of human papillomavirus (hpv). | 2006 | 16998682 |
| differential expression and activation of nf-kappab family proteins during oral carcinogenesis: role of high risk human papillomavirus infection. | oral cancer is one of the most common cancers in india and south-east asian region consisting of more than 50% of all malignant tumors. along with many known risk factors, infection of human papillomavirus (hpv) has been associated with the development of oral cancer and is suggested to modulate host cell transcription. reciprocally, cellular transcription factors, such as nf-kappab and ap-1 are known to modulate the expression of viral and other genes involved in the development of cancer. in t ... | 2006 | 16998793 |
| a content analysis of news coverage of the hpv vaccine by u.s. newspapers, january 2002-june 2005. | genital human papillomavirus (hpv) is the most common sexually transmitted infection in the united states. of the 100 hpv types, hpv type 16 and hpv type 18 have been demonstrated to cause cervical cancer. two pharmaceutical manufacturers have developed and tested hpv vaccines and are applying to the fda for licensure. this research describes the content of hpv vaccine information contained in news articles. the lexis-nexis database was used to identify 25 articles on hpv that were published in ... | 2006 | 16999634 |
| women's reactions to hpv diagnosis: insights from in-depth interviews. | research links certain types of the human papillomavirus (hpv) to subsequent development of cervical cancer. women (n=52) recently diagnosed with hpv following an abnormal pap smear participated in in-depth interviews to explore their knowledge of hpv, their emotional responses to diagnosis, and the extent of their disclosure of their hpv diagnosis to others. women had many knowledge deficits about hpv. emotions related primarily to stigma, fear, self-blame, powerlessness, and anger. most women ... | 2006 | 17000613 |
| oligonucleotide microarray with rd-pcr labeling technique for detection and typing of human papillomavirus. | currently, screening for high-risk human papillomavirus (hpv) infection remains an important health concern throughout the world, because of the close association between certain types of hpv and cervical cancer. in this study, we explore the possibility of using approximately 70mer oligonucleotide microarray for detection and genotyping of hpv. the approximately 70mer type-specific oligonucleotide probes of four different types hpv were designed by using biological software array designer 2.0, ... | 2006 | 16479359 |
| comparison of cervical and blood t-cell responses to human papillomavirus-16 in women with human papillomavirus-associated cervical intraepithelial neoplasia. | human papillomaviruses (hpvs) are obligate epithelial pathogens and typically cause localized mucosal infections. we therefore hypothesized that t-cell responses to hpv antigens would be greater at sites of pathology than in the blood. focusing on hpv-16 because of its association with cervical cancer, the magnitude of hpv-specific t-cell responses at the cervix was compared with those in the peripheral blood by intracellular cytokine staining following direct ex vivo stimulation with both virus ... | 2006 | 17026720 |
| identification of susceptibility loci for cervical carcinoma by genome scan of affected sib-pairs. | cervical cancer is caused by a combination of environmental and genetic risk factors. infection by oncogenic types of human papillomavirus is recognized as the major environmental risk factor and epidemiological studies indicate that host genetic factors predispose to disease development. a number of genetic susceptibility factors have been proposed, but with exception of the human leukocyte antigen chla, class ii, have not shown consistent results among studies. we have performed the first geno ... | 2006 | 17035246 |
| recurrent respiratory papillomatosis causing chronic stridor and delayed speech in an 18-month-old boy. | recurrent respiratory papillomatosis is a relatively uncommon disease that presents clinically with symptoms ranging from hoarseness to severe dyspnea. human papilloma virus types 6 and 11 are important in the etiology of papillomas and are most probably transmitted from mother to child during birth. although spontaneous remission is frequent, pulmonary spread and/or malignant transformation resulting in death has been reported. co2 laser evaporation of papillomas and adjuvant drug therapy using ... | 2006 | 17036092 |
| atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion: diagnostic performance, human papillomavirus testing, and follow-up results. | current guidelines recommend colposcopy rather than high-risk human papillomavirus (hpv) testing for the evaluation of abnormal cervical cytology interpreted as "atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion" (asc-h) based on data from the atypical squamous cells of undetermined significance/low-grade squamous intraepithelial lesion (ascus/lsil) triage study (alts), which indicated that asc-h had a significantly greater frequency of high-risk hpv positivity a ... | 2006 | 16136595 |
| immortalization of epstein-barr virus-negative human b lymphocytes with minimal chromosomal instability. | the genes required for immortalization of human b cells infected by epstein-barr virus are multiple, and the precise mechanism of this process remains to be elucidated. in the present study hpv16 e6 and e7 were retrovirally transduced into human primary b cells stimulated by cd40-cd40l interaction, thereby establishing an epstein-barr virus negative immortalized human b cell line, which continued to proliferate for more than 2 years (100 population doublings). the established cell line had a hig ... | 2006 | 17040288 |
| critical roles for non-prb targets of human papillomavirus type 16 e7 in cervical carcinogenesis. | high-risk human papillomaviruses (hpv) encode two oncogenes, e6 and e7, expressed in nearly all cervical cancers. in vivo, hpv-16 e7 has been shown to induce multiple phenotypes in the context of transgenic mice, including cervical cancer. e7 is a multifunctional protein known best for its ability to inactivate the tumor suppressor prb. to determine the importance of prb inactivation by e7 in cervical cancer, we pursued studies with genetically engineered mice. e7 expression in estrogen-treated ... | 2006 | 17018593 |
| re: human papillomavirus infection and incidence of squamous cell and basal cell carcinomas of the skin. | 2006 | 17018790 | |
| detection of human papillomavirus in cervical cell specimens by hybrid capture and pcr with different primers. | the purpose of this study was to compare hybrid capture assay with pcrs using different primers for the l1, e6-e7 regions for the detection of human papillomavirus (hpv) genome. one hundred twenty-five cervical smears with normal (n=42) and abnormal (n=83) cytology were investigated. those at high-risk for hpv were studied by hybridization antibody capture assay and pcr with the pu-1m/pu-2r primers. target dna from the hpv l1 region was amplified by spf10 primer set and home-pcr with my09/my11 p ... | 2006 | 17019439 |
| human papillomavirus type 5 e6 oncoprotein represses the transforming growth factor beta signaling pathway by binding to smad3. | mechanisms of cellular transformation associated with human papillomavirus type 5 (hpv5), which is responsible for skin carcinomas in epidermodysplasia verruciformis (ev) patients, are poorly understood. using a yeast two-hybrid screening and molecular and cellular biology experiments, we found that hpv5 oncoprotein e6 interacts with smad3, a key component in the transforming growth factor beta1 (tgf-beta1) signaling pathway. hpv5 e6 inhibits smad3 transactivation by destabilizing the smad3/smad ... | 2006 | 17020941 |
| cross-sectional comparison of an automated hybrid capture 2 assay and the consensus gp5+/6+ pcr method in a population-based cervical screening program. | in this cross-sectional study, clinical performances of the hybrid capture 2 assay using an automated instrument (i.e., rapid capture system) (hc2-rcs) and the high-risk human papillomavirus gp5+/6+ pcr-enzyme immunoassay (eia) test were compared using cervical scrape specimens from 8,132 women that participated in a population-based screening trial. the hc2-rcs test scored significantly more samples positive (6.8%) than the gp5+/6+ pcr-eia (4.8%) (p < 0.0005). this could be attributed largely t ... | 2006 | 17021097 |
| [the swedish institute of infectious disease control starts a registry for follow up of vaccination against human papillomavirus]. | 2006 | 17022190 | |
| cell detachment model for an antibody-based microfluidic cancer screening system. | we consider cells bound to the floor of a microfluidic channel and present a model of their flow-induced detachment. we approximate hydrodynamic force and cell elastic response using static finite-element simulation of a single cell. detachment is assumed to occur when hydrodynamic and adhesive forces are roughly equal. the result is extended to multiple cells at the device level using a sigmoidal curve fit. the model is applied to a microfluidic cancer-screening device that discriminates betwee ... | 2006 | 17022683 |
| inhibition of dna replication of human papillomavirus by artificial zinc finger proteins. | recently, we demonstrated that plant dna virus replication was inhibited in planta by using an artificial zinc finger protein (azp) and created azp-based transgenic plants resistant to dna virus infection. here we apply the azp technology to the inhibition of replication of a mammalian dna virus, human papillomavirus type 18 (hpv-18). two azps, designated azp(hpv)-1 and azp(hpv)-2, were designed by using our nondegenerate recognition code table and were constructed to block binding of the hpv-18 ... | 2006 | 16699021 |
| topical 5% 5-fluorouracil cream in the treatment of plantar warts: a prospective, randomized, and controlled clinical study. | topical 5-fluorouracil (5-fu) is an antineoplastic antimetabolite that inhibits dna and rna synthesis, thereby preventing cell replication and proliferation. this mechanism of action may allow topical 5-fu to be utilized in the treatment of human papilloma virus (hpv). we conducted a study comparing 5% 5-fu cream under tape occlusion versus tape occlusion alone in 40 patients presenting with plantar warts. nineteen out of 20 patients (95%) randomized to 5% 5-fu with tape occlusion had complete e ... | 2006 | 16703777 |
| human papillomavirus infection: an old disease, a new vaccine. | human papillomavirus (hpv) infection is common and causes a wide spectrum of disease. with recent advances in the development of prophylactic hpv vaccines, it is likely that these will be licensed for use in the near future. this review focuses on the science behind hpv vaccines, published clinical trial results for both prophylactic and therapeutic hpv vaccines, important issues relevant to implementation and cost-effectiveness models of hpv vaccination programs. it may be that an hpv vaccine t ... | 2006 | 16704468 |
| human papillomavirus vaccines. | a wealth of epidemiological and molecular evidence has led to the conclusion that virtually all cases of cervical cancer and its precursor intra-epithelial lesions are a result of infection with one or other of a subset of genital human papillomaviruses (hpvs) suggesting that prevention of infection by prophylactic vaccination would be an effective anti-cancer strategy. the papillomaviruses cannot be grown in large amounts in culture in vitro, but the ability to generate hpv virus like particles ... | 2006 | 16710836 |
| controversies in the public health approach to keratinocyte carcinomas. | keratinocyte carcinomas are very common cancers in fair-skinned populations throughout the world. the term 'keratinocyte carcinoma' includes basal and squamous cell carcinoma of the skin, but not other cancers that may be included under the more ambiguous term 'nonmelanoma skin cancer'. incidence and mortality are important for assessing impact and recent research suggests that these cancers are increasing in incidence in young adults. there is evidence to suggest that this trend may be due, in ... | 2006 | 16712708 |
| structural and thermodynamic basis for the enhanced transcriptional control by the human papillomavirus strain-16 e2 protein. | strain 16 of the human papillomavirus is responsible for the largest number of cases of cervical cancers linked to this virus, and the e2 protein is the transcriptional regulator of all viral genes. we present the first structure for the dna binding domain of hpv16 e2 bound to dna, and in particular, to a natural cognate sequence. the nmr structure of the protein backbone reveals that the overall conformation remains virtually unchanged, and chemical shift analysis of the protein bound to a shor ... | 2006 | 16716065 |
| prevalence and risk factors of hpv infection among high-risk rural and urban lithuanian women. | to investigate the prevalence, persistence and risk factors of high oncogenic risk human papillomavirus (hpv) among urban and rural women of reproductive age coming to consult a gynaecologist. | 2006 | 16717474 |
| reduced incidence of cervical cancer in mothers of sons with allergic rhinoconjunctivitis, asthma or eczema. | because infection with human papillomavirus is a necessary cause of cervical cancer, it is likely that host immunological factors involved in defense against such infections are important for susceptibility to this cancer. by examining associations between allergy in sons and cervical cancer in their mothers, we aimed to test for genetic components involved in both allergy and cervical cancer development. women born in sweden between 1932 and 1960 with at least 1 son with medical records from mi ... | 2006 | 16721814 |
| human papillomavirus testing in primary cervical screening and abnormal papanicolaou management. | human papillomavirus (hpv) infection is a necessary cause of cervical cancer; hpv testing capitalizes on this fact to maximize the clinical benefits of cervical screening and abnormal papanicolaou (pap) test management. hpv tests in combination with pap tests are 96% to 100% sensitive for detection of cervical intraepithelial neoplasia (cin) 2/3 and cancer. however, because hpv infection is common in young women and most commonly transient, hpv testing is not recommended as part of primary cervi ... | 2006 | 16729900 |
| human papillomavirus: the burden of infection. | vaccines for preventing human papillomavirus (hpv) infection are far along in clinical development and testing, and hold great promise for reducing hpv infections and hpv-associated disease. hpv is the most common sexually transmitted infection in the united states, affecting an estimated 75% of the u.s. population. hpv infection is highly prevalent in sexually active adolescents and young adults. sexual activity is the most important risk factor for infection, with 64% to 82% of sexually active ... | 2006 | 16729902 |
| [clinical analysis of the infection with human papillomavirus in women]. | to determine the relationship between human papillomavirus infection, cervical carcinoma, pre-cancerous lesion and condyloma acuminatum. | 2006 | 16816863 |
| hpv16 variants in squamous intraepithelial lesions in human immunodeficiency virus-negative and -positive brazilian women. | emerging evidence supports the role of human papillomavirus (hpv) intratype variations in the development of cervical lesions in immunocompetent women, but few studies investigated hpv16 variants in human immunodeficiency virus (hiv)-positive women. this is the first study in brazil evaluating hpv16 variants in women with (n = 19) and without (n = 22) hiv infection, as well as cervical lesions. although non-european variants presented an almost 3-fold increase (13.6% vs. 36.8%) among hiv-positiv ... | 2006 | 16817777 |
| p16 and ki67 immunostaining is a useful adjunct in the assessment of biopsies for hpv-associated anal intraepithelial neoplasia. | p16 is a tumor suppressor gene product, shown to be overexpressed in most cervical carcinomas and dysplasias associated with high-risk human papilloma virus (hpv) infection. hpv is also associated with anal squamous dysplasias and carcinomas. significant interobserver and intraobserver variation exists in the interpretation of biopsies for anal intraepithelial neoplasia (ain). this study was undertaken to assess the potential role of p16 and ki67 immunohistochemical expression in refining the di ... | 2006 | 16819320 |