Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| multiple inflammatory markers in patients with significant coronary artery disease. | several inflammatory biomarkers are linked to cardiovascular risk. in order to investigate their coexistence and relative responses, several established and two novel markers (lactoferrin and the terminal complement complex), representing infection and central components of inflammation, were measured simultaneously in patients undergoing first-time coronary angiography. | 2007 | 16935369 |
| is periodontal infection behind the failure of antibiotics to prevent coronary events? | the use of antimicrobial agents to prevent coronary events is under debate. they have reduced cardiovascular events in some studies, but in others, their effect has not been distinguishable from that of placebo. in addition to chlamydophila (chlamydia) pneumoniae as a target pathogen, very few other microbes or infections have been targeted, although an association for instance between cardiovascular disease and periodontitis has been established. in our recent pilot study, long-term clarithromy ... | 2007 | 16876173 |
| dual role of infections as risk factors for coronary heart disease. | the aim of the study was to explore whether exposure to microbial agents determines the prevalence of acute coronary events. | 2007 | 16780845 |
| racial difference in endothelial function: role of the infective burden. | there is much evidence to suggest the existence of racial differences between blacks and whites in the behaviour of endothelial function. infective state, sustained by viral or bacterial agents, may injure the endothelial surface favouring the onset and progression of atherosclerotic process, mainly by an inflammatory mechanism. the aim of the study was to investigate endothelial function, expressed as brachial flow-mediated vasodilation (fmv), in black and white healthy subjects, along with ant ... | 2007 | 16712854 |
| chlamydia pneumoniae infection of brain cells: an in vitro study. | inspired by the suggested associations between neurological diseases and infections, we determined the susceptibility of brain cells to chlamydia pneumoniae (cpn). murine astrocyte (c8d1a), neuronal (nb41a3) and microglial (bv-2) cell lines were inoculated with cpn. infection was established by immunofluorescence and real-time pcr at various time points. productive infection was assessed by transferring medium of infected cells to a detection layer. finally, apoptosis and necrosis post-infection ... | 2007 | 16621171 |
| evaluation of different nucleic acid amplification techniques for the detection of m. pneumoniae, c. pneumoniae and legionella spp. in respiratory specimens from patients with community-acquired pneumonia. | the number of pathogens involved in community-acquired pneumonia, with varying susceptibilities to antimicrobials, is numerous constituting an enormous challenge for diagnostic microbiology. differentiation of infections due to streptococcus pneumoniae and those due to mycoplasma pneumoniae, chlamydophila pneumoniae, or l. pneumophila as well as those due to viruses is essential to allow correct decisions concerning the antibiotics to be administered. the sensitivity and specificity of real-time ... | 2008 | 18378345 |
| immunoproteomic identification and serological responses to novel chlamydia pneumoniae antigens that are associated with persistent c. pneumoniae infections. | the controversial discussion about the role of chlamydia pneumoniae in atherosclerosis cannot be solved without a reliable diagnosis that allows discrimination between past and persistent infections. using a proteomic approach and immunoblotting with human sera, we identified 31 major c. pneumoniae ags originating from 27 different c. pneumoniae proteins. more than half of the proteins represent chlamydia ags not described previously. using a comparative analysis of spot reactivity pmp6, omp2, g ... | 2008 | 18390732 |
| surfactant protein d: not just for the lung anymore. | 2008 | 18390823 | |
| probing the functional tolerance of the b subunit of escherichia coli atp synthase for sequence manipulation through a chimera approach. | a dimer of 156-residue b subunits forms the peripheral stator stalk of eubacterial atp synthase. dimerization is mediated by a sequence with an unusual 11-residue (hendecad) repeat pattern, implying a right-handed coiled coil structure. we investigated the potential for producing functional chimeras in the b subunit of escherichia coli atp synthase by replacing parts of its sequence with corresponding regions of the b subunits from other eubacteria, sequences from other polypeptides having simil ... | 2008 | 18395001 |
| comparison of serological tests for detection of immunoglobulin m antibodies to chlamydophila pneumoniae. | to evaluate an enzyme immunoassay (eia) (anilab c. pneumoniae) for detecting anti-chlamydophila pneumoniae-specific igm antibody, by comparing it with an elisa, hitazyme c. pneumoniae, and a micro-immunofluorescence (mif) test. | 2008 | 18399867 |
| detection of infectious agents by polymerase chain reaction in human aortic wall. | several studies have been suggested that infectious agents may induce or progress the process of atherosclerosis in humans. in the present study, the samples of visually healthy human aortic wall were examined for the presence of chlamydia pneumoniae, mycoplasma pneumoniae, helicobacter pylori, herpes simplex virus (hsv), and cytomegalovirus (cmv). | 2008 | 18402822 |
| intragastric primary infection sensitizes to lung reinfection in a chlamydia pneumoniae mouse model. | the most frequently used infection route in chlamydia pneumoniae animal models is intranasal (i.n.), while the intragastric (i.g.) infection route has not been studied previously. the aim of the present study was to examine the course of chlamydia pneumoniae infection in mice infected via the i.g. route and to compare the outcome of i.n. reinfection in these mice to i.n. reinfection of primarily i.n. infected mice. c57bl/6jbom mice were used, and the infection was monitored using chlamydial cult ... | 2008 | 18433949 |
| chlamydophila pneumoniae changes iron homeostasis in infected tissues. | many bacteria, including chlamydophila pneumoniae (c. pneumoniae), are dependent on iron (fe) for their growth. however, it is not known whether bacterial infections affect gastrointestinal uptake and uptake of trace elements in infected tissues. a human c. pneumoniae strain adapted to c57bl/6j mice was used to study hepcidin gene expression in the liver and divalent metal transporter 1 (dmt1) content in the liver and intestine and whether fe is concomitantly changed in serum, liver, and intesti ... | 2008 | 18436480 |
| chlamydophila pneumoniae and endothelial activation: the smoke that precedes the fire of atherosclerosis? | 2008 | 18436797 | |
| characterization and intracellular localization of putative chlamydia pneumoniae effector proteins. | we here describe four proteins of chlamydia pneumoniae, which might play a role in host-pathogen interaction. the hypothetical bacterial proteins cpn0708 and cpn0712 were detected in chlamydia pneumoniae-infected host cells by indirect immunofluorescence tests with polyclonal antisera raised against the respective proteins. while cpn0708 was localized within the inclusion body, cpn0712 was identified in the inclusion membrane and in the surrounding host cell cytosol. cpn0712 colocalizes with act ... | 2008 | 18449565 |
| the effect of chlamydia pneumoniae on the expression of peroxisome proliferator-activated receptor-gamma in vascular smooth muscle cells. | this study was designed to investigate the change of peroxisome proliferator-activated receptor gamma (ppargamma) after the infection of the human coronary artery smooth muscle cells (hcsmcs) with chlamydia pneumoniae (c. pneumoniae) and the effect of ppargamma agonist on the expression of ppargamma of c. pneumoniae-infected hcsmcs. | 2008 | 18452259 |
| chlamydia pneumoniae infection in il-10 knock out mice: accelerated clearance but severe pulmonary inflammatory response. | in interleukin-10 knock out (il-10 ko) mice, accelerated clearance of pulmonary chlamydia pneumoniae infection was observed. on the other hand, the histopathological changes in lung tissue were more pronounced in il-10 ko mice at all time points after infection and repeated infection than in the wild type mice. both ex vivo induced antigen-specific proliferation as well as production of proinflammatory cytokines by splenocytes were higher in il-10 ko mice than in wt mice. also, intrapulmonary pr ... | 2008 | 18456450 |
| a meta-analysis of antibiotic use for the secondary prevention of cardiovascular diseases. | a number of clinical trials have examined whether antibiotics decrease the incidence of adverse events in patients with cardiovascular diseases. however, results have occasionally been conflicting, and a meta-analysis may provide additional clarification. | 2008 | 18464945 |
| chlamydia pneumoniae infection and hyperlipidaemia-induced expression of p50 and c-fos in the heart of c57bl6j mice. | the objective of this study was to investigate infection by chlamydia pneumoniae (c. pneumoniae) and the combined effects of an atherogenic diet on the expression of ap-1 or the subunit of ap-1 (c-fos) and nf-kappab or the subunit of nf-kappab (p50) in myocardial cells of c57bl/6j mice. | 2008 | 18468197 |
| extreme temperature tolerance of a hyperthermophilic protein coupled to residual structure in the unfolded state. | understanding the mechanisms that dictate protein stability is of large relevance, for instance, to enable design of temperature-tolerant enzymes with high enzymatic activity over a broad temperature interval. in an effort to identify such mechanisms, we have performed a detailed comparative study of the folding thermodynamics and kinetics of the ribosomal protein s16 isolated from a mesophilic (s16(meso)) and hyperthermophilic (s16(thermo)) bacterium by using a variety of biophysical methods. a ... | 2008 | 18471828 |
| anti-chlamydophila pneumoniae antibodies as associated factor for carotid atherosclerosis in patients with aids. | atherosclerosis is a multifactor disease. lately, infectious factors such as c. pneumoniae have been found to be involved. to determine whether the infection by c. pneumoniae is a risk factor for atherosclerosis in patients with aids. case-control study on 43 patients with aids under haart (16 cases and 27 controls). to document atherosclerosis, a carotid and transcranial doppler ultrasound was performed. anti-c pneumoniae antibodies were searched using a microimmunofluorescence test for igm and ... | 2008 | 18473791 |
| association of alzheimer's disease and chlamydophila pneumoniae. | this paper critically reviews the association of infection by chlamydophila pneumoniae (c. pneumoniae) and alzheimer's disease (ad). the aging population has increased interest in finding the cause of ad, but studies have yielded contradictory results that are likely due to varying diagnostic tools and different uses of diagnostic tests. knowledge of ad's characteristics, risk factors, and hypothesized etiologies has expanded since alois alzheimer's initial description of ad. epidemiologic and p ... | 2008 | 18474399 |
| rapid and simple diagnosis of chlamydophila pneumoniae pneumonia by an immunochromatographic test for detection of immunoglobulin m antibodies. | to evaluate a newly developed immunochromatographic test (the myset test) for the detection of chlamydophila pneumoniae-specific immunoglobulin m antibodies, the results obtained by the myset test were compared with those obtained by two serological tests. the sensitivity and specificity of the myset test were 100% and 92.9%, respectively. the myset test is rapid and simple to use and is thought to be a useful tool for the selection of appropriate antibiotic therapy. | 2008 | 18480232 |
| maternal serum chlamydia pneumoniae antibodies and crp levels in women with preeclampsia and gestational hypertension. | to determine whether chlamydia pneumoniae antibodies and highly sensitive c-reactive protein (hscrp) levels in maternal sera are associated with preeclampsia or gestational hypertension. | 2008 | 18484420 |
| chlamydophila pneumoniae and the etiology of late-onset alzheimer's disease. | sporadic, late-onset alzheimer's disease (load) is a non-familial, progressive neurodegenerative disease that is now the most common and severe form of dementia in the elderly. that dementia is a direct result of neuronal damage and loss associated with accumulations of abnormal protein deposits in the brain. great strides have been made in the past 20 years with regard to understanding the pathological entities that arise in the ad brain, both for familial ad ( approximately 5% of all cases) an ... | 2008 | 18487846 |
| increased infiltration of chlamydophila pneumoniae in the vessel wall of human veins after perfusion. | several studies have suggested an association between chlamydophila pneumoniae (cp) infection and atherosclerosis. a recent study detected cp dna in the saphenous vein of 12% of all patients before bypass grafting and in 38% of failed grafts. we used a system in which human veins were perfused with autologous blood under arterial pressure. | 2008 | 18489582 |
| severe asthma exacerbation: role of acute chlamydophila pneumoniae and mycoplasma pneumoniae infection. | chlamydophila pneumoniae and mycoplasma pneumoniae are associated with acute exacerbation of bronchial asthma (aeba). the aim of this study was to evaluate the correlation between these acute bacterial infections and the severity of aeba. | 2008 | 18513407 |
| inflammatory- and immune responses in relation to bacterial replication in mice following re-infections with chlamydophila pneumoniae. | investigation of chronic infections with chlamydophila pneumoniae. | 2008 | 18516711 |
| [periodontitis and cardiovascular disease]. | the prevalence of periodontitis and cardiovascular disease (cvd) is high. a mixed infectious biofilm etiology of periodontitis is known but not fully established in cvd. cofactors; smoking habits, stress, ethnicity, genetics, socioeconomics and age contribute to both diseases. the objectives of this report are to summarize factors in regards to cvd and periodontitis that are clinically relevant. the hypothesis behind a relationship between the two conditions can be founded in (i) shared infectio ... | 2008 | 18517068 |
| selective cyclooxygenase inhibitors prevent the growth of chlamydia pneumoniae in hl cells. | the effects of the selective cyclooxygenase (cox) inhibitors sc-560 and ptpbs were studied in chlamydia pneumoniae-infected hl cell cultures. chlamydia pneumoniae growth and viability were assessed by quantifying inclusions and re-passages. cox-1 and cox-2 mrna expression in hl cells during chlamydial infection was quantified with real-time polymerase chain reaction. sc-560 (10 microg/ml) and ptpbs (18 microg/ml) completely inhibited the growth of c. pneumoniae and the effect was dose-dependent ... | 2008 | 18524544 |
| amalgamation of chlamydia pneumoniae inclusions with lipid droplets in foam cells in human atherosclerotic plaque. | chlamydia pneumoniae (chlamydophila pneumoniae) infect macrophages and accelerates foam cell formation in in vitro experiments, but whether this might occur in human atherosclerosis is unknown. in the present study, we examined 17 carotid artery segments, obtained by endarterectomy, in which the presence of c. pneumoniae was confirmed by both polymerase chain reaction and immunohistochemistry. electron microscopy demonstrated the presence of structures with the appearance of elementary, reticula ... | 2008 | 18528704 |
| minocycline treatment results in reduced oral steroid requirements in adult asthma. | the tetracycline antibiotics have pleiotropic anti-inflammatory properties that may explain their therapeutic benefit in rheumatoid arthritis and acne. as these agents suppress both cellular and humoral immune responses, they may be of benefit in treating asthma and other allergic disorders. the purpose of this study was to determine whether minocycline therapy of asthma has steroid sparing effects beyond its inherent antibiotic properties. adult asthmatic patients (n = 17) were treated with min ... | 2008 | 18534087 |
| no evidence of involvement of chlamydia pneumoniae in lung cancer by means of quantitative real-time polymerase chain reaction. | chlamydia pneumoniae, an obligate intracellular pathogen, is well-known as etiological agent of acute respiratory infections; the repeated or prolonged exposure to chlamydial antigens may promote the persistence of c. pneumoniae in the respiratory tract leading to chronic diseases, such as chronic obstructive pulmonary disease and asthma. the predilection of c. pneumoniae to cause respiratory tract infections combined with its persistent nature suggest that it might play a role in lung cancer. t ... | 2008 | 18547487 |
| real-time polymerase chain reaction and laser capture microdissection: an efficient combination tool for chlamydophila pneumoniae dna quantification and localization of infection in atherosclerotic lesions. | chlamydophila pneumoniae has been implicated in atherosclerosis, but the role of this obligate intracellular pathogen in the development of the above pathology is still unclear. in particular, its presence and quantitative distribution within lesional areas has not yet been defined. we studied 18 carotid biopsies obtained from patients undergoing endoartherectomy. by laser microdissection (lcm), two different sites (intra-plaque and plaque-adjacent areas) were taken from each lesion, and the pre ... | 2008 | 18547488 |
| abdominal aortic aneurysm and the impact of infectious burden. | little is known about the biological processes causing aortic aneurysm rupture. chronic chlamydophila pneumoniae infection has been suggested as a possible contributing factor to the development and expansion of abdominal aortic aneurysm (aaa). the importance of infection in aaa may be related to the previous pathogen burden, that is, the number of significant titres of antibodies against infectious pathogens rather than to single infectious agents. the aim of this study was to examine the relat ... | 2008 | 18547827 |
| antigen-induced immunomodulation in the pathogenesis of atherosclerosis. | atherosclerosis is a chronic inflammatory disorder characterised by the accumulation of monocytes/macrophages, smooth muscle cells, and lymphocytes within the arterial wall in response to the release of proinflammatory molecules. such accumulation results in the formation of the atherosclerotic plaque, which would eventually evolve to complications such as total artery occlusion, rupture, calcification, or aneurysm. although the molecular mechanism responsible for the development of atherosclero ... | 2008 | 18551190 |
| benefit-risk assessment of telithromycin in the treatment of community-acquired pneumonia. | the purpose of this review is to assess the benefits and risks associated with the use of the ketolide antibacterial telithromycin, currently licensed for the treatment of adults with mild to moderate community-acquired pneumonia (cap). telithromycin is active against both the major (streptococcus pneumoniae, haemophilus influenzae and moraxella catarrhalis) and atypical/intracellular (chlamydophila pneumoniae, legionella pneumophila and mycoplasma pneumoniae) cap pathogens. it is associated wit ... | 2008 | 18558790 |
| molecular detection of parachlamydia-like organisms in cerebrospinal fluid of patients with multiple sclerosis. | the presence of chlamydia-like organism dna was investigated by polymerase chain reaction (pcr) in cerebrospinal fluid (csf) samples from 27 patients previously found positive for chlamydia pneumoniae dna: 12 with multiple sclerosis (ms), grouped according to clinical and magnetic resonance imaging (mri) evidence of disease activity, 8 with other inflammatory neurological disorders and 7 with non-inflammatory neurological disorders. pcr evidence of chlamydia-like organisms in csf was observed on ... | 2008 | 18562511 |
| role of atypical bacteria in children undergoing tonsillectomy because of severely recurrent acute tonsillopharyngitis. | the aim of this study was to verify the frequency of atypical bacterial infections in children undergoing tonsillectomy because of severely recurrent acute tonsillopharyngitis (at) and the possible benefit of surgery in cases in which mycoplasma pneumoniae and chlamydophila pneumoniae seem to play a role in causing the recurrences. a total of 118 patients (76 males; mean age +/- standard deviation, 6.67 +/- 3.31 years) were enrolled: 59 underwent tonsillectomy because of severely recurrent at an ... | 2008 | 18566843 |
| influenza virus infection and risk of acute myocardial infarction. | increasing evidences have shown that pathogens might promote atherosclerosis and trigger acute myocardial infarction (ami). but the conclusions from various studies on the correlation between previous influenza virus (iv) infection and ami were inconsistent. we conducted a case-control study to assess the association of previous iv infection and ami. questionnaire survey was conducted to collect information about demographic characteristics and heart disease risk factors. fasting blood sample wa ... | 2008 | 18568394 |
| chlamydia pneumoniae antibodies in various subtypes of ischemic stroke in indian patients. | as infections occur more frequently in developing countries, we carried out this prospective case-control study, to establish the association, if any, between c. pneumoniae antibodies and ischemic stroke particularly in relation to its subtypes. | 2008 | 18571201 |
| chlamydia pneumoniae infection results in generalized bone loss in mice. | osteoporosis is associated with a general bone loss. whether infections could contribute to osteoporosis is not known. chlamydia pneumoniae causes chronic infections and produces potentially bone resorptive cytokines. the effect of c. pneumoniae infection was investigated in vivo in 10-week old mice (c57bl/6) and in vitro in the human osteoblast-like cell line hfob 1.19 (hfob). bone mineral density (bmd) was measured before and 16 days after infection. c. pneumoniae-infected mice had decreased ( ... | 2008 | 18640288 |
| [chronic infection is one of the etiologies for digestive diseases and atherosclerosis]. | 2008 | 18646591 | |
| detection of 11 common viral and bacterial pathogens causing community-acquired pneumonia or sepsis in asymptomatic patients by using a multiplex reverse transcription-pcr assay with manual (enzyme hybridization) or automated (electronic microarray) detection. | community-acquired pneumonia (cap) and sepsis are important causes of morbidity and mortality. we describe the development of two molecular assays for the detection of 11 common viral and bacterial agents of cap and sepsis: influenza virus a, influenza virus b, respiratory syncytial virus a (rsv a), rsv b, mycoplasma pneumoniae, chlamydophila pneumoniae, legionella pneumophila, legionella micdadei, bordetella pertussis, staphylococcus aureus, and streptococcus pneumoniae. further, we report the ... | 2008 | 18650351 |
| the relationship between chlamydophila pneumoniae igg titer and coronary atherosclerosis. | the role of chlamydophila pneumoniae (cp) in the progression of atherosclerosis is controversial. also no sufficient angiographic study is available about the impact of cp infection on severity and intensity of coronary atherosclerosis. we investigated the relation between cp igg antibody titers and severity and intensity of coronary atherosclerosis | 2008 | 18651417 |
| evaluation and optimization of a commercial enzyme linked immunosorbent assay for detection of chlamydophila pneumoniae iga antibodies. | serologic diagnosis of chlamydophila pneumoniae (cpn) infection routinely involves assays for the presence of igg and igm antibodies to cpn. although iga antibodies to cpn have been found to be of interest in the diagnosis of chronic infections, their significance in serological diagnosis remains unclear. the microimmunofluorescence (mif) test is the current method for the measurement of cpn antibodies. while commercial enzyme linked immunosorbent assays (elisa) have been developed, they have no ... | 2008 | 18655722 |
| one question on the work on antinuclear antibody levels in patients with hodgkin's and non-hodgkin's lymphoma. | 2008 | 18663350 | |
| interrelationship of interleukin 6, c-reactive protein and chlamydia pneumoniae igg antibodies in patients with acute coronary syndromes. | inflammation due to infection could be associated with the development of acute coronary syndromes, clinical manifestations of ongoing atherosclerosis in vessel walls. our aim was determine whether interleukin 6, c-reactive protein and chlamydia pneumoniae igg antibodies are connected with the development of acute coronary syndromes, to evaluate their interrelationship and to examine whether they are predictive of new events and mortality. | 2008 | 18672698 |
| inhibition of lymphocyte cd3 expression by chlamydophila pneumoniae infection. | since lymphocytes are a major immune cell besides macrophages in the development of atherosclerosis, interaction between lymphocytes and chlamydophila pneumoniae may contribute to the pathogenesis of chronic inflammatory diseases associated with c. pneumoniae. in this regard, we examined a possible alteration of cd3 expression of human lymphocyte molt-4 cells by c. pneumoniae infection. the expression levels of cd3 molecules of lymphocyte molt-4 cells were significantly decreased by c. pneumonia ... | 2008 | 18674609 |
| the intracellular accumulation of phagocytic and epithelial cells and the inhibitory effect on chlamydophila (chlamydia) pneumoniae of telithromycin and comparator antimicrobials. | the intracellular accumulation of telithromycin was measured and compared with ciprofloxacin, clarithromycin, minocycline and erythromycin. the activities of telithromycin, clarithromycin and minocycline against chlamydophila (chlamydia) pneumoniae were compared in an intracellular killing assay. maximal telithromycin accumulation (mean intracellular/extracellular concentration ratio) ranged from 6.7 (a549 lung epithelial cells) to 11.8 (thp-1 monocytic cells). this ratio was similar to that of ... | 2008 | 18676220 |
| retinoic acid prevents chlamydia pneumoniae-induced foam cell development in a mouse model of atherosclerosis. | chlamydia pneumoniae, a common respiratory pathogen, has been associated with cardiovascular disease. c. pneumoniae infection accelerates atherosclerotic lesion development in hyperlipidemic animals. retinoic acid, an anti-oxidant, inhibits infection of endothelial cells by c. pneumoniae. the present study demonstrated that retinoic acid suppresses the acceleration of foam cell lesion development induced by c. pneumoniae in hyperlipidemic c57bl/6j mice. retinoic acid treatment had no effect on f ... | 2008 | 18678272 |
| [production and application of chlamydia pneumoniae-specific monoclonal antibody]. | the purified elementary bodies of c. pneumoniae tw-183 were used for immunization of male balb/c mice, the spleen cells of these mice were fused with sp2/0 cells and the hybrid cells were cloned by limiting dilution. one clone that secreted the c. pneumoniae monoclonal antibody (cpn-mcab) stably was obtained finally. the cpn-mcab belonged to igg2b class and anti-cpn-momp; the outcome of micro-immunofluorescence showed its weak cross reaction with the c. psittaci elementary body but it has no cro ... | 2008 | 18693451 |
| can phages cause alzheimer's disease? | alzheimer's disease (ad) is a progressive neurodegenerative disorder with progressive dementia. multiple processes have been implicated in ad, notably including abnormal beta-amyloid production, tau hyperphosphorylation and neurofibrillary tangles (nfts), synaptic pathology, oxidative stress, inflammation, protein processing or misfolding, calcium dyshomeostasis, aberrant reentry of neurons into the cell cycle, cholesterol synthesis, and effects of hormones or growth factors. the complexity of t ... | 2008 | 18701222 |
| phylogenetic comparison of the known chlamydia trachomatis sigma(66) promoters across to chlamydia pneumoniae and chlamydia caviae identifies seven poorly conserved promoters. | we used four different phylogenetic footprinting programs and the six chlamydial species with publicly available whole genome sequences to analyze the 12 known sigma(66) promoters of chlamydia trachomatis that phylogenetically footprinted negative in our previous paper. the analysis showed that 7 of the 12 promoters were poorly conserved across c. trachomatis, chlamydia pneumoniae and chlamydia caviae. interestingly, the associated gene sets for these seven promoters were homologs and the gene o ... | 2008 | 18708139 |
| characterization of the putative type iii secretion atpase cdsn (cpn0707) of chlamydophila pneumoniae. | type iii secretion (t3s) is utilized by a wide range of gram-negative bacterial pathogens to allow the efficient delivery of effector proteins into the host cell cytoplasm through the use of a syringe-like injectisome. chlamydophila pneumoniae is a gram-negative, obligate intracellular pathogen that has the structural genes coding for a t3s system, but the functionality of the system has not yet been demonstrated. t3s is dependent on atpase activity, which catalyzes the unfolding of proteins and ... | 2008 | 18708502 |
| lack of association between serum antibodies of chlamydia pneumoniae infection and the risk of lung cancer. | 2008 | 18720403 | |
| temporal delay of peak t-cell immunity determines chlamydia pneumoniae pulmonary disease in mice. | severe chlamydial disease typically occurs after previous infections and results from a hypersensitivity response that is also required for chlamydial elimination. here, we quantitatively dissected the immune and disease responses to repeated chlamydia pneumoniae lung infection by multivariate modeling with four dichotomous effects: mouse strain (a/j or c57bl/6), dietary protein content (14% protein and 0.3% l-cysteine-0.9% l-arginine, or 24% protein and 0.5% l-cysteine-2.0% l-arginine), dietary ... | 2008 | 18725423 |
| chlamydia pneumoniae and acute aortic syndrome: a call for a multi-institutional study. | chlamydia pneumoniae (cp) infection is strongly associated with coronary artery disease, as well as with atherosclerosis of the carotid and peripheral arteries. however, the role of cp in the pathogenesis of aortic disease remains controversial. our present experience suggests no correlation between a current infection with c. pneumoniae and acute aortic dissection. well-designed large prospective studies are needed in order to clarify the pathophysiologic role of cp infection in acute and chron ... | 2008 | 18754273 |
| analysis of children with chlamydophila (chlamydia) pneumoniae and mycoplasma pneumoniae respiratoryinfections by real-time pcr assay and serological tests. | we examined 73 children with respiratory infections for chlamydophila (chlamydia) pneumoniae and mycoplasma pneumoniae using real-time pcr assay and serological tests. c. pneumoniae and m. pneumoniae infections were found in 11 (15.1%) and 6 (8.2%) cases, respectively. the sensitivities and specificities of real-time pcr versus definite diagnosis of acute infection were 63.6% and 100% for c. pneumoniae, and 100% and 100% for m. pneumoniae, respectively. c. pneumoniae pcr-negative results appeare ... | 2008 | 18754321 |
| serological diagnosis of chlamydophila pneumoniae infection in greek copd patients by microimmunofluorescence and elisa. | the possible role of chlamydophila pneumoniae infection in episodes of acute exacerbation of copd (aecopd) was described in 4-34% of copd patients, but never in greece. the possible association of chronic c. pneumoniae infection with disease severity is under study, with a diversity of results reported. | 2008 | 18758425 |
| arterial intima-media thickness in 13-year-old adolescents and previous antichlamydial antimicrobial use: a retrospective follow-up study. | children with persistent chlamydia pneumoniae infection may be at increased risk for atherosclerosis. the impact of antimicrobial therapy for primary prevention of atherosclerotic cardiovascular disease is unsolved. | 2008 | 18762502 |
| chlamydophila felis cf0218 is a novel tmh family protein with potential as a diagnostic antigen for diagnosis of c. felis infection. | chlamydophila felis is a causative agent of acute and chronic conjunctivitis and pneumonia in cats (feline chlamydiosis). also, c. felis is a suspected zoonotic agent of such diseases as non-chlamydia trachomatis conjunctivitis in humans, although this is controversial. at present, there is no serodiagnostic system that specifically detects c. felis infection conveniently. current systems use antigens such as lipopolysaccharide that cross-react with all chlamydia species. in addition, it is diff ... | 2008 | 18768674 |
| innate immune responses during respiratory tract infection with a bacterial pathogen induce allergic airway sensitization. | the original hygiene hypothesis predicts that infections should protect against asthma but does not account for increasing evidence that certain infections might also promote asthma development. a mechanistic reconciliation of these findings has not yet emerged. in particular, the role of innate immunity in this context is unclear. | 2008 | 18774395 |
| a light at the end of the tunnel of inflammation in obstructive airway diseases? | 2008 | 18779188 | |
| asthma management by monitoring sputum neutrophil count. | we report the utility of quantitative cell counts in sputum in monitoring therapy of a patient with poorly controlled asthma. recurrent neutrophilic bronchitis without an eosinophilic bronchitis led to the identification of chlamydophila pneumoniae as the cause of bronchitis and asthma exacerbation. serial examination of blood and sputum by polymerase chain reaction for c pneumoniae helped to prevent exacerbations by prophylactic antibiotic therapy, reduce the dose of prednisone and inhaled cort ... | 2008 | 18779198 |
| outer and inner membrane proteins compose an arginine-agmatine exchange system in chlamydophila pneumoniae. | most chlamydial strains have a pyruvoyl-dependent decarboxylase protein that converts l-arginine to agmatine. however, chlamydiae do not produce arginine, so they must import it from their host. chlamydophila pneumoniae has a gene cluster encoding a putative outer membrane porin (cpn1033 or aaxa), an arginine decarboxylase (cpn1032 or aaxb), and a putative cytoplasmic membrane transporter (cpn1031 or aaxc). the aaxc gene was expressed in escherichia coli producing an integral cytoplasmic membran ... | 2008 | 18790867 |
| cloning, expression and purification of truncated chlamydia trachomatis outer membrane protein 2 (omp2) and its application in an elisa assay. | although a simple and direct method does not exist for the detection of chlamydial infections, there are situations in which reliable serological tests, with sensitivity related to a specific antigen, could be helpful. | 2008 | 18791281 |
| the association of c (-260)-->t polymorphism in cd14 promoter and chlamydia pneumoniae infection in ischemic stroke patients. | the c (-260) --> t polymorphism has been reported to regulate cd14 gene expression. it has also been implicated in atherosclerotic diseases, and in addition, it could be a genetic factor responsible for interindividual differences in the susceptibility to chlamydia pneumoniae infection. this case-control study is aimed at evaluating the association between cd14 promoter polymorphisms, frequency of persistent c pneumoniae infection, and anti-chlamydial heat shock protein 60 (chsp60) induction in ... | 2008 | 18794053 |
| permanent atrial fibrillation in patients without structural heart disease is not associated with signs of infection by chlamydia pneumoniae and helicobacter pylori. | the objective of this study was to explore the role of chlamydia pneumoniae and helicobacter pylori infections in patients with idiopathic permanent atrial fibrillation. | 2008 | 18795586 |
| induction of inos by chlamydophila pneumoniae requires myd88-dependent activation of jnk. | innate immune cells produce no via inducible no synthase (inos) in response to certain infections or upon stimulation with cytokines such as ifn-gamma and tnf. no plays an important role in host defense against intracellular bacteria including chlamydophila pneumoniae as a result of its microbicidal activity. in myd88-deficient mice, which succumb to c. pneumoniae infection, inos induction is impaired 6 days postinfection, although pulmonary levels of ifn-gamma and tnf are elevated as in wild-ty ... | 2008 | 18799752 |
| chlamydophila pneumoniae dna and mrna transcript levels in peripheral blood mononuclear cells and cerebrospinal fluid of patients with multiple sclerosis. | chlamydophila pneumoniae dna and mrna transcripts were investigated by pcr and rt-pcr in fresh csf and pbmc specimens co-cultured in hep-2 cell lines and collected from 14 patients with definite rr ms and 19 patients with other inflammatory (oind) and non-inflammatory (nind) neurological controls. a positivity for c. pneumoniae dna and mrna was detected in csf and pbmcs of 9 rr ms patients (64.2%) with evidence of disease activity, whereas only 3 controls were positive for chlamydial dna. these ... | 2008 | 18572268 |
| association of hyperhomocysteinemia and chlamydia pneumoniae infection with carotid atherosclerosis and coronary artery disease in japanese patients. | an elevated plasma level of homocysteine (hcy) and infection by chlamydia pneumoniae (c. pneumoniae) have been suggested as independent risk factors for carotid atherosclerosis (ca) and coronary artery disease (cad), but the mechanisms involved are unclear. we investigated the correlation between positivity for antibody to c. pneumonia (anti-c. pneumoniae) and the hcy level in patients with ca and cad. the total plasma homocysteine (thcy) concentration was determined in 99 patients with ca and 3 ... | 2008 | 18574661 |
| asthma, infection and antibiotic. | 2008 | 18581720 | |
| improving outcomes of elderly patients with community-acquired pneumonia. | community-acquired pneumonia (cap) is a major cause of morbidity and mortality in elderly patients. therefore, efforts to optimize the healthcare process for patients with cap are warranted. an organized approach to management is likely to improve clinical results. assessing the severity of cap is crucial to predicting outcome, deciding the site of care, and selecting appropriate empirical therapy. unfortunately, current prognostic scoring systems for cap such as curb-65 (confusion, uraemia, res ... | 2008 | 18582147 |
| studies suggest an association between maternal periodontal disease and pre-eclampsia. | in this systematic review, several types of infections are identified and investigated: urinary tract infection, periodontal disease, chlamydia pneumoniae infection, hiv infection, malaria and other persistent bacterial and viral infections. separate analyses were conducted for each of them. this summary review will only focus on the link between pre-eclampsia and periodontitis, which was just a part of the original systematic review. | 2008 | 18584002 |
| identification of selected respiratory pathogens in endodontic infections. | to determine whether endodontic infections could harbor common etiologic agents of respiratory infections such as streptococcus pneumoniae and chlamydia pneumoniae. | 2008 | 18585629 |
| [study on gene cloning of chlamydial pneumonia cpn0308 and its endogenous localization]. | to clone cpn0308 gene from clamyida pneumonia and express its fusion protein, to make antibodies to fusion protein gst-cpn0308, and to further localize endogenous protein preliminarily using antibodies raised with cpn0308 fusion protein. | 2008 | 18589613 |
| [early diagnosis using recombinant protein of immunodominant region gene of chlamydial protease-like activity factor from chlamydophila pneumoniae]. | to clone and express the immunodominant domain gene of the chlamydial protease-like activity factor (cpaf) from chlamydophila pneumoniae. the value of the recombinant protein was evaluated for diagnosing early infection. | 2008 | 18590240 |
| natural killer t cells are critical for dendritic cells to induce immunity in chlamydial pneumonia. | we previously showed an important role of natural killer t cells (nkt) in skewing the adaptive t cell immunity to chlamydia pneumoniae (cpn), an intracellular bacterial lung infection, but the mechanism remains unclear. | 2008 | 18596223 |
| modulation of cytokine and beta-defensin 2 expressions in human gingival fibroblasts infected with chlamydia pneumoniae. | human beta-defensin 2 is an antimicrobial peptide that is produced by several epithelial cells after stimulation with micro-organisms and inflammatory mediators. gram-negative bacteria, which are typically detected in periodontal pockets in periodontitis, elicit a stronger antibacterial peptide response of human beta-defensin 2 by epithelial cells. in this study, we investigated whether chlamydia pneumoniae is able both to enter and grow in human gingival fibroblasts (hgf), to modify the product ... | 2008 | 18602070 |
| a prospective comparison of nursing home-acquired pneumonia with hospital-acquired pneumonia in non-intubated elderly. | there are no prospective comparison of the etiology and clinical outcome between hospital-acquired pneumonia (hap) and nursing home-acquired pneumonia (nhap) in non-intubated elderly. this study prospectively evaluated the etiology of hap and nhap in non-intubated elderly. a prospective cohort study was carried out in a rural region of japan where the population over 65 years of age represents 30% of the population. a total of 108 patients were enrolled. there were 33 patients with hap and 75 wi ... | 2008 | 18602805 |
| community-acquired pneumonia in children in lambarene, gabon. | community-acquired pneumonia (cap) accounts for more than two million deaths per year in children < 5 years of age. recognition of pathogens is vital for guiding antibiotic treatment. in gabon, no epidemiologic data on childhood cap were available to help guide antibiotic therapy. we conducted a prospective, hospital-based, cross-sectional survey at the albert schweitzer hospital, lambarene, gabon, to assess the importance of atypical organisms (chlamydia pneumoniae, mycoplasma pneumoniae, borde ... | 2008 | 18606773 |
| chlamydophila pneumoniae hflx belongs to an uncharacterized family of conserved gtpases and associates with the escherichia coli 50s large ribosomal subunit. | predicted members of the hflx subfamily of phosphate-binding-loop guanosine triphosphatases (gtpases) are widely distributed in the bacterial kingdom but remain virtually uncharacterized. in an attempt to understand mechanisms used for regulation of growth and development in the chlamydiae, obligate intracellular and developmentally complex bacteria, we have begun investigations into chlamydial gtpases; we report here what appears to be the first analysis of a hflx family gtpase using a predicte ... | 2008 | 18957606 |
| chronic chlamydia pneumoniae infection and bronchial asthma: is there a link? | besides well-defined environmental causes, accumulating evidence suggests that respiratory tract infections play an important role in the pathogenesis of asthma. among these chlamydia pneumoniae infection has been discussed as possibly inducing the development of asthma. | 2008 | 18974486 |
| [case of infectious mononucleosis with suspected primary coinfection with chlamydophila (chlamydia) pneumoniae and epstein-barr virus]. | a 26-year-old male was hospitalized with fever and pharyngeal pain. liver dysfunction and an increase in the percentage of atypical lymphocytes in the peripheral blood were detected. computed tomography showed pneumonia involving the right lung and synpneumonic pleural effusion. serum immunological tests showed positive results for epstein-barr virus (ebv) viral capsid antigen (vca) igm and igg antibodies and chlamydophila (chlamydia) pneumoniae (c. pneumoniae) igm and iga antibodies on admissio ... | 2008 | 18975589 |
| tlr/myd88 and liver x receptor alpha signaling pathways reciprocally control chlamydia pneumoniae-induced acceleration of atherosclerosis. | experimental and clinical studies link chlamydia pneumoniae infection to atherogenesis and atherothrombotic events, but the underlying mechanisms are unclear. we tested the hypothesis that c. pneumoniae-induced acceleration of atherosclerosis in apolipoprotein e (apoe)(-/-) mice is reciprocally modulated by activation of tlr-mediated innate immune and liver x receptor alpha (lxralpha) signaling pathways. we infected apoe(-/-) mice and apoe(-/-) mice that also lacked tlr2, tlr4, myd88, or lxralph ... | 2008 | 18981139 |
| chlamydia pneumoniae-induced foam cell formation requires myd88-dependent and -independent signaling and is reciprocally modulated by liver x receptor activation. | chlamydia pneumoniae is detected by macrophages and other apcs via tlrs and can exacerbate developing atherosclerotic lesions, but how that occurs is not known. liver x receptors (lxrs) centrally control reverse cholesterol transport, but also negatively modulate tlr-mediated inflammatory pathways. we isolated peritoneal macrophages from wild-type, tlr2, tlr3, tlr4, tlr2/4, myd88, trif, myd88/trif, and ifn regulatory factor 3 (irf3) ko mice, treated them with live or uv-killed c. pneumoniae in t ... | 2008 | 18981140 |
| is activity against "atypical" pathogens necessary in the treatment protocols for community-acquired pneumonia? issues with combination therapy. | the "atypical pathogens" reviewed include legionella pneumophila, chlamydophilia pneumoniae, and mycoplasma pneumoniae. urinary antigen tests are the most frequently used tests for legionella species and show good specificity and reasonable sensitivity. for m. pneumoniae, detection of immunoglobulin m, used for the past decade, has substantially improved diagnostic specificity and has simplified testing. for c. pneumoniae, there is no consensus on a simplified test that can be commonly used, and ... | 2008 | 18986295 |
| role of bacteria in acute exacerbations of chronic obstructive pulmonary disease. | infections are major causes of acute exacerbations of chronic obstructive pulmonary disease (copd) which result in significant mortality and morbidity. the primary aim of the study was to determine the microbiological spectrum including atypical agents in acute exacerbations. the secondary aim was to evaluate resistance patterns in the microorganisms. | 2008 | 18990975 |
| [modern methods of laboratory diagnostics of chlamydia infections]. | need for further improvement of methods for verification of etiological agent of urogenital and respiratory chlamydiosis on the basis of increased biotechnological requirements to antigens for serological reactions, primers for pcr assay (refinement of connection of primers with microorganism's zones of genome most significant for its life activity or formation of most diagnostically significant complexes of primers), and selection of cultivating conditions considering the predicted features of ... | 2008 | 18819420 |
| the essential role of the copn protein in chlamydia pneumoniae intracellular growth. | bacterial virulence determinants can be identified, according to the molecular koch's postulates, if inactivation of a gene associated with a suspected virulence trait results in a loss in pathogenicity. this approach is commonly used with genetically tractable organisms. however, the current lack of tools for targeted gene disruptions in obligate intracellular microbial pathogens seriously hampers the identification of their virulence factors. here we demonstrate an approach to studying potenti ... | 2008 | 18830244 |
| evaluation of direct immunofluorescence test for diagnosis of upper respiratory tract infection by chlamydia pneumoniae. | chlamydia pneumoniae causes a variety of respiratory infections and is involved in cardiovascular diseases. diagnosis of c. pneumoniae infection currently relies on antibody detection by microimmunofluorescence (mif), which has limited use, and is the retrospective diagnosis for acute infection. | 2008 | 18843873 |
| chlamydia pneumoniae iga and elevated level of il-6 may synergize to accelerate coronary artery disease. | a strong association of cytokines [interleukin (il)-6, il-2 and tumor necrosis factor (tnf)-alpha] with atherosclerosis is well documented. however, their role in chlamydia pneumoniae (cp)-associated progression of coronary artery disease (cad) is not well studied. hence we evaluated the presence of these cytokines in cad patients positive for cp immunoglobulin (ig) a (low, medium, and high levels) cad and compared with controls. also the physiological and baseline characteristics in il-6 positi ... | 2008 | 18922388 |
| [acute pericarditis as an organic manifestation of the acute infection chlamydia pneumoniae]. | the article presents the case of a 52-year-old patient who was treated in our clinic for very serious acute chlamydia pericarditis (a chlamydia pneumonia infection) with a large pericardial exudate. the clinical progression was that of an acute systematic illness. the article describes the differential diagnostic algorithm used during hospitalisation and the corresponding treatments provided. similar cases are very rarely described in the literature. the discussion includes an analysis of the po ... | 2008 | 18924348 |
| rifalazil retains activity against rifampin-resistant mutants of chlamydia pneumoniae. | rifampin-resistant mutants of the obligate intracellular pathogen chlamydia pneumoniae were isolated and characterized, including strains that contained multiple mutations in the rpob gene encoding the rifampin binding site. the highest mic of rifampin against a mutant strain exceeded 100 microg/ml, whereas the highest mic of rifalazil was 0.125 microg/ml. derivatives of rifalazil (new chemical entities; nces) showed from 2 approximately 4 fold lower mics, as well as 2 approximately 8 fold lower ... | 2008 | 18997387 |
| helicobacter pylori infection and arterial stiffness in patients with type 2 diabetes mellitus. | epidemiologic studies have suggested possible atherogenic roles for such pathogens as chlamydia pneumoniae, helicobacter pylori (hp), cytomegalovirus, and herpes simplex virus. the aim of the present study was to examine the relationship between seropositivity of antibodies to hp (hp infection) and arterial stiffness determined by pulse wave velocity (pwv) in 130 patients (73 men and 57 women) with type 2 diabetes mellitus without a history of cardiovascular disease. the prevalence of hp infecti ... | 2008 | 19013302 |
| assessment of the bioequivalence of two formulations of clarithromycin extended-release 500-mg tablets under fasting and fed conditions: a single-dose, randomized, open-label, two-period, two-way crossover study in healthy jordanian male volunteers. | clarithromycin extended-release tablets are indicated for the treatment of adults with acute maxillary sinusitis caused by haemophilus influenzae, moraxella catarrhalis, or streptococcus pneumoniae; acute bacterial exacerbation of chronic bronchitis due to h influenzae, haemophilus parainfluenzae, m catarrhalis, or s pneumoniae; or community acquired pneumonia due to h influenzae, h parainfluenzae, m catarrhalis, s pneumoniae, chlamydia pneumoniae, or mycoplasma pneumoniae. | 2008 | 19014838 |
| limited role for c. pneumoniae, cmv and hsv-1 in cerebral large and small vessel atherosclerosis. | aims: to explore whether chlamydia pneumoniae, cytomegalovirus and herpes simplex virus type 1 could be detected in large and small cerebral arteries, as well as in an area of brain parenchyma where white matter lesions (leukoaraiosis) can be found, in patients with clinically unmanifested cerebrovascular atherosclerosis. methods and results( arterial specimens from the basilar artery and middle cerebral artery, and brain samples from the basal ganglia and periventricular white matter were obtai ... | 2008 | 19018307 |
| high immunoglobulin a seropositivity for combined chlamydia pneumoniae, helicobacter pylori infection, and high-sensitivity c-reactive protein in coronary artery disease patients in india can serve as atherosclerotic marker. | atherosclerosis is increasingly recognized as a chronic inflammatory disease. a variety of infectious agents (chlamydia pneumoniae, helicobacter pylori, and cytomegalovirus [cmv]) and inflammatory marker such as high-sensitivity c-reactive protein (hs-crp) have been found to be associated with atherosclerosis and its consequences. there is a need to know about the type and burden of infection in coronary artery disease (cad) patients and the level of hs-crp in india as there is growing evidence ... | 2008 | 19037586 |
| chlamydial lps and high-sensitivity crp levels in serum are associated with an elevated body mass index in patients with cardiovascular disease. | seropositivity for chlamydia pneumoniae has been associated with an elevated body mass index (bmi). our aim was to study if serum chlamydial lipopolysaccharide (clps), c. pneumoniae antibodies and high-sensitivity c-reactive protein (hscrp) levels are associated with bmi. | 2008 | 19039061 |