Publications

the use of density gradient centrifugation for the separation of germinated from nongerminated spores.		1972	4626090
action of supermutagens on the transforming dna of bacillus subtilis.		1972	4626133
[corneal ulcer and causative organisms].		1972	4626203
variability of dna content in individual cells of bacillus.		1972	4626246
[mutants of bacillus subtilis marburg with late blockage in their sporulation].		1972	4626264
in-use contamination of intravenous solutions in flexible plastic containers.		1972	4626385
[permeation of glycerol and sporulation of bacillus subtilis].		1972	4626434
[study of metabolism of glycerol by two mutants of bacillus subtilis].		1972	4626435
induction of reproductive phage in b. subtilis by triethylene-melamine or methyl-nitro-nitrosoguanidine and their reaction with deoxyribonucleic acid.		1972	4626486
similarity in the size and number of ribosomal proteins from different prokaryotes.	the ribosomal proteins from nine species of prokaryotes have been compared by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. the stained gels were scanned spectrophotometrically, the weight and number average molecular weights were calculated, and the detailed distribution of the proteins as a function of molecular weight was determined. by all of these criteria, the ribosomal proteins from all the species closely resembled each other, despite differences in the pa ...	1972	4626500
fate of transforming deoxyribonucleic acid after uptake by competent bacillus subtilis: size and distribution of the integrated donor segments.	a series of sheared donor-recipient complex samples were prepared from a competent bacillus subtilis culture which had been transformed by using (3)h, (2)h-labeled deoxyribonucleic acid. measurement of the molecular weights and buoyant densities of these samples led to the following conclusions. (i) the average size of the single-stranded donor segment which is integrated is 2.8 x 10(6). (ii) these donor segments are not randomly distributed in the recipient genome, but are clustered in groups, ...	1972	4626501
effects of fatty acids on growth and envelope proteins of bacillus subtilis.	fatty acids of different chain lengths were added to cultures of bacillus subtilis growing in nutrient sporulation medium, and the effects of these fatty acids on growth, oxygen uptake, adenosine triphosphate (atp) concentration, and membrane protein composition were examined. all fatty acids inhibited growth, the effect being reduced in the presence of glycolytic compounds and reversed by transfer to medium without fatty acids. the inhibition of growth was correlated with a reduction in both th ...	1972	4626502
mode of action of phleomycin on bacillus subtilis.	phleomycin appears to act on the cell wall and membrane to induce the release of membrane-associated deoxyribonucleic acid (dna) and the degradation of the dna. degradation occurs in a series of energy-requiring endonuclease and exonuclease reactions. these produce, first, single-strand breaks, then double-strand breaks, and finally almost complete solubilization of the cellular dna. the in vivo inhibition of dna synthesis by phleomycin is probably a secondary effect caused by the destruction of ...	1972	4626504
rate of depurination of native deoxyribonucleic acid.		1972	4626532
intergenotic transformation of the bacillus subtilis genospecies.	a multiple auxotrophic derivative of bacillus subtilis 168 (strain br151 carrying lys-3, trpc2, metb10) was transformed with deoxyribonucleic acid (dna) isolated from b. subtilis 168, bacillus amyloliquefaciens h, b. subtilis hsr, bacillus pumilus, and bacillus licheniformis. transformation with heterologous dna occurred at a very low frequency for the three auxotrophic markers. heterologous transformation to rifampin resistance was 100 to 1,000 times more efficient than transformation to protot ...	1972	4626541
[regulatory events during the initiation of sporulation in bacillus subtilis].		1972	4626594
[studies on biologically active substances formed by bacilli. 3. isolation of rabbit serum calcium decreasing substance formed by b. subtilis k and its properties].		1972	4626621
[studies on biologically active substances formed by bacilli. iv. toad heart function promoting substance from culture filtrate of b. subtilis k].		1972	4626622
[demonstration of reaginic (ige) and precipitating (igg) antibodies in the serum of a patient allergic to bacillus subtilis enzymes].		1972	4626628
multiple penicillin-binding components in bacillus subtilis, bacillus cereus, staphylococcus aureus, and escherichia coli.		1972	4626716
transport of dicarboxylic acids in bacillus subtilis. inducible uptake of l-malate.		1972	4626722
mammalian deoxyribonucleases acting on damaged dna.		1972	4626725
the metabolic fate of -sulphobenzylpenicillin in rats.		1972	4626858
serologic estimation of thymine dimers in the deoxyribonucleic acid of bacterial and mammalian cells following irradiation with ultraviolet light and postirradiation repair.		1972	4626880
a deoxyribonucleic acid polymerase i-deficient mutant of bacillus subtilis.		1972	4626881
regulation of aromatic amino acid biosynthesis in bacillus subtilis 168. i. evidence for and characterization of a trifunctional enzyme complex.		1972	4626882
[influence of cell density on the sporulation and formation of unusually phosphorylated substances in bacillus subtilis].		1972	4627018
regulation of acetohydroxyacid synthetase in bacillus subtilis.		1972	4627023
temperature-sensitive mutants of b. subtilis defective in deoxyribonucleotide synthesis.		1972	4627024
an acceptor-dependent polyglycerolphosphate polymerase.	a method for the extraction of polyglycerolphosphate polymerase from bacillus subtilis membranes is described. further purification by ion-exchange chromatography yields an enzyme fraction totally dependent on the addition of a heat-stable factor for activity. this factor was isolated from cell membranes and acts as an acceptor of glycerolphosphate units. the acceptor contains glycerolphosphate, glucosamine, and fatty acids, but its precise chemical structure has not yet been elucidated.	1972	4627027
factors affecting the biosynthesis of mitomycins by streptomyces caespitosus.		1972	4627036
enzymatic determination of structure of singly branched hexaose dextrins formed by liquefying -amylase of bacillus subtilis.		1972	4627053
relationship of structures and microbiological activities of the 16-membered macrolides.		1972	4627057
dna of ciliated protozoa. 3. binding of rna polymerase and denaturation at the ends of the low-molecular weight dna in stylonychia.		1972	4627105
visualization of reinitiated chromosomes in bacillus subtilis.		1972	4627106
membrane attachment of the replication origins of a multifork (dichotomous) chromosome in bacillus subtilis.		1972	4627160
the effect of osmotic shocking upon bacillus subtilis transformation.		1972	4627195
methods for the assessment of microbial populations recovered from enclosed aerosols.		1972	4627206
rapid assays for the detection and determination of sparse populations of bacteria and bacteriophage t7 with radioactively labelled homologous antibodies.		1972	4627207
physical, chemical and morphological studies of spore coat of bacillus subtilis.		1972	4627210
utilization of collagenous by-products from the meat packing industry: production of single-cell protein by the continuous cultivation of bacillus megaterium.	the conditions for continuous cultivation of bacillus megaterium on a collagen-derived substrate (sp-100) were determined. the optimum conditions of temperature, ph, and dilution rate were 34 c, ph 7.0, and 0.25/hr, respectively. increasing the substrate concentration in plain tap water resulted in proportional increases in the productivity of cell mass from 0.6 g per liter per hr at 1% substrate to 1.8 g per liter per hr at 10% substrate; however, the protein content of the biomass decreased fr ...	1972	4627231
proteolytic components of alkaline proteases of bacillus strains. zymograms and electrophoretic isolation.		1972	4627237
the structure and antigenic analysis of amorphous inclusion bodies induced by potato virus x.		1972	4627341
arrest of host dna synthesis in bacillus subtilis infected with phage phi e.		1972	4627342
folic acid and the methylation of homocysteine by bacillus subtilis.	1. cell-free extracts of bacillus subtilis synthesize methionine from serine and homocysteine without added folate. the endogenous folate may be replaced by tetrahydropteroyltriglutamate or an extract of heated escherichia coli for the overall c(1) transfer, but tetrahydropteroylmonoglutamate is relatively inactive. 2. extracts of b. subtilis contain serine transhydroxymethylase and 5,10-methylenetetrahydrofolate reductase, which are non-specific with respect to the glutamate content of the fola ...	1972	4627401
effect of adenine on the riboflavin-sensitized photoreaction. ii. effect of adenine on the photodynamic inactivation of transforming deoxyribonucleic acid in the presence of riboflavin.		1972	4627422
effect of adenine on the riboflavin-sensitized photoreaction. 3. effect of adenine on the photodegradation of guanine residues in deoxyribonucleic acid in the presence of riboflavin.		1972	4627423
the mechanism of wall synthesis in bacteria. the organization of enzymes and isoprenoid phosphates in the membrane.	1. the synthesis of peptidoglycan and teichoic acids by cell-free preparations from bacillus licheniformis a.t.c.c. 9945 and bacillus subtilis n.c.t.c. 3610 has been studied under a variety of conditions. 2. it was shown that poly(glycerol phosphate) is synthesized through a lipid intermediate, and it is concluded from this and other work that all major bacterial wall polymers are formed in a similar manner through such intermediates. 3. close interrelation between the synthesis of peptidoglycan ...	1972	4627447
transcription after bacteriophage spp1 infection in bacillus subtilis.	the role of the host polymerase in bacillus subtilis infected with phage spp1 was studied in vivo with regard to production of phage-specific and host-specific ribonucleic acid (rna) and to phage yield. evidence is presented that the subunit(s) of b. subtilis rna polymerase which is sensitive to rifampin and streptolydigin is necessary at all times during infection for phage production. the synthesis of phage rna and the phage yield in strains resistant to either antibiotic were unaffected by th ...	1972	4627475
rna polymerase from bacillus amyloliquefaciens.		1972	4627588
the effect of growth conditions on the mucopeptide composition of walls of bacillus subtilis w23.		1972	4627685
[changes in nitrogen metabolism in the mouse brain evoked by metabolic products of bacillus subtilis 110].		1972	4627778
frozen human plasma and bacterial contamination.		1972	4627788
properties of n-methyl-n'-nitro-n-nitrosoguanidine and its action on bacillus subtilis transforming dna.		1972	4627843
evidence for specific inhibition of translocation of aminoacyl-transfer ribonucleic acid by the pretreatment of ribosomes of bacillus subtilis with p-chloromercuribenzoate.	the biological activities of bacillus subtilis ribosomes pretreated with 0.5, 1, or 2 mmp-chloromercuribenzoate (pcmb) were examined. the ribosomes treated with 1 or 2 mm pcmb lost their capacity to synthesize polyphenylalanine as well as the capacity to bind phenylalanyl-transfer ribonucleic acid. on the other hand, approximately 70% of the polyphenylalanine synthesis capacity was lost with ribosomes pretreated with 0.5 mm pcmb. this inhibition was found to be due to a specific inhibition of th ...	1972	4627920
activity of deoxyribonucleic acid fragments of defined size in bacillus subtilis transformation.	the transforming activity of bacillus subtilis deoxyribonucleic acid (dna) that had been sheared and purified with respect to size by sucrose gradient sedimentation is given as a function of the dna molecular weight. it is shown (i) that fragments of median molecular weight 1.2 x 10(6) have finite activity (10(-4)), (ii) that the shape of the activity-versus-molecular weight function is qualitatively similar to that observed previously for diplococcus pneumoniae, and (iii) that this shape preclu ...	1972	4627923
potassium content during growth and sporulation in bacillus subtilis.	vegetative and sporulating cells of bacillus subtilis retain a higher level of internal potassium than do nonsporulating stationary-phase cells. the addition of manganese to nonsporulating stationary-phase cells, at concentrations required for sporulation, rapidly stimulates uptake and net accumulation of potassium and induces sporulation.	1972	4627924
differential amino acid requirements for sporulation in bacillus subtilis.	the amino acid requirements for sporulation were studied by use of auxotrophic mutants of bacillus subtilis 168. cells were grown to t(0) in medium containing the test amino acid and were then transferred to a minimal medium lacking that amino acid. omission of leucine caused no reduction in sporulation. omission of methionine, lysine, and phenylalanine appeared to cause reduced levels of sporulation, and sporulation was completely inhibited when isoleucine, tryptophan, and threonine were omitte ...	1972	4627926
effect of diethyl pyrocarbonate on the biological acitivity of deoxyribonucleic acids isolated from bacteriophages.		1972	4627929
bacillus cereus-induced fluid accumulation in rabbit ileal loops.	the usefulness of the ligated rabbit ileal loop as an experimental model of bacillus cereus food poisoning was investigated. positive responses, as measured by fluid accumulation in the loop, were obtained from 19 of 22 strains of b. cereus. four of six strains of b. thuringiensis also elicited fluid accumulation, but eight strains of other bacillus spp. failed to evoke a response. the growth medium employed markedly affected the ability of a given strain of b. cereus to provoke a response. brai ...	1972	4627964
identification of a germination system involved in the heat injury of bacillus subtilis spores.	bacillus subtilis a spores were injured by exposure to heat treatments of 110 to 132 c. injury was demonstrated by the inability to form colonies on fortified nutrient agar (fna) unless the medium was supplemented with cacl(2) and na(2) dipicolinate (cna). a preliminary heat treatment fully heat-activated the spores, was not lethal, and did not prevent injury by subsequent secondary heat treatment. exposure of heat-activated spores to 122 c reduced germination in fna. the primary germination age ...	1972	4627967
ultrahigh-temperature activation of a low-temperature bacillus subtilis spore germination system.	exposure of bacillus subtilis a spores to 115 to 125 c for several seconds activated a low-temperature germination system that remained dormant after a heat treatment that activated the normal l-alanine- and glucose-stimulated germination systems. the low-temperature germination system was characterized by an optimum temperature lower than that of the l-alanine or glucose germination systems (30 c versus 45 c), germination in the absence of exogenous germination stimulants, and the capacity for ...	1972	4627968
an osmotically fragile mutant of bacillus subtilis with an active membrane-associated phospholipase a 1 .	by use of a newly developed procedure for the isolation of mutants with osmotically fragile protoplasts, a mutant of bacillus subtilis was isolated that has a very active enzyme system for the catabolism of phospholipids via the sequential action of a phospholipase a(1) (ec 3.1.1.4) and a lysophospholipase (ec 3.1.1.5). the wild-type bacteria contain no detectable phospholipase a(1) activity, but do contain a protein that specifically inhibits the phospholipase a(1) in the mutant. this protein m ...	1972	4628095
chromosomal location of mutations affecting sucrose metabolism in bacillus subtilis marburg.		1972	4628133
a modified microtome for slicing polyacrylamide gels.		1972	4628175
bacterial inhibition of ureteral smooth muscle contractility. i. the effect of common urinary pathogens and endotoxin in an in vitro system.		1972	4628237
study of antimicrobial activity of chamomile oil.		1972	4628248
eclipse of viral dna infectivity in spo1-infected bacillus subtilis.		1972	4628252
[food hygienic studies of sulfite. i. effects of sulfite on the growth of microorganisms].		1972	4628269
[food hygienic studies of sulfite. 3. effects of sulphus oxides on microbial hydrolytic enzymes and digestive enzymes].		1972	4628270
the regulation of the butanediol cycle in bacillus subtilis.		1972	4628298
micro method for determination of blocked nh 2 - terminal amino acids of protein: application to identification of acetylserine of phosphoglycerate kinase and pyroglutamic acid of glucose 6-phosphate dehydrogenase.		1972	4628357
the genetics of bacteriophage spo1.		1972	4628367
[production of xylanase by bacillus subtilis].		1972	4628431
[lysogenisation of bacillus subtilis using phage and prophage-dna].		1972	4628433
[aminoacyl-t rna-synthetases and cell division in a temperature sensitive filiform mutant of bacillus subtilis sb 19].		1972	4628434
in vitro transcription.		1972	4628438
antigen-antibody complexes in the immune response. i. analysis of the effectiveness of complexes on the primary antibody response.		1972	4628463
a determination of the efficiency of uptake of sp50 dna by competent cells of b. subtilis.		1972	4628471
deoxyribonucleic acid polymerase activity in a deoxyribonucleic acid polymerase i-deficient mutant of bacillus subtilis infected with temperature bacteriophage spo2.	increased deoxyribonucleic acid (dna) polymerase activity is found in soluble extracts from a polymerase i-negative mutant of bacillus subtilis after infection with temperate phage spo2, or after induction of spo2 prophage in lysogenic derivatives of this mutant. no increased enzyme activity is found after spo2 infection in the presence of chloramphenicol. infection of the polymerase-negative mutant with the dna-negative sus mutant spo2 l244 gives no increased enzyme activity, whereas infection ...	1972	4628507
proteolytic conversion in vitro of b. subtilis vegetative rna polymerase into the homologous spore enzyme.		1972	4628580
utilization of single l-amino acids as sole source of carbon and nitrogen by bacteria.		1972	4628671
influences of environmental ionic strength, ph, and magnesium ion on bactericidal and lytic activities of -lysin on bacillus subtilis.		1972	4628672
bacterial ribonucleic acid in the frog brain after a bacterial peritoneal infection.	frogs were injected intraperitoneally with bacteria, and the rna of the brains (which have protective barriers against the bacteria used) was extracted. part of the rna was bacterial rna apparently resulting from the transcription of dna transferred from bacteria to the brain cells.	1972	4628710
some properties of atp dependent deoxyribonucleases from normal and rec-mutant strains of bacillus subtilis.		1972	4628739
hyperprotease-producing mutants of bacillus subtilis.	a number of mutants of bacillus subtilis producing high levels of extracellular protease have been isolated. analysis of culture supernatants of these mutants has shown that the total amount of proteolytic activity is elevated from 16- to 37-fold over the wild strain. the elevated activity was due to a simultaneous increase in both the neutral and alkaline protease. all of the mutants genetically analyzed were found linked to the argc4 marker by pbs-1 transduction analysis.	1972	4628743
5-bromouracil-tolerant mutants of bacillus subtilis.	5-bromouracil (bu)-tolerant mutants of bacillus subtilis 23 (thy his) have been isolated. several classes of tolerant mutants were obtained by a sequential selection procedure. the classes can be distinguished by their relative bu tolerance as well as several other phenotypic characteristics. the mutants can grow for an extended period of time in minimal medium supplemented with amino acids and bu, in which the sensitive parental strain (bu(+)) undergoes rapid cell death. both mutants but-1 and ...	1972	4628747
genetic regulation of cell division initiation in bacillus subtilis.	the growth and division properties of a temperature-sensitive mutant of bacillus subtilis defective in the initiation of cell division have been studied. log-phase cells transferred from 30 to 45 c continue to increase in length but fail to initiate new divisions. deoxyribonucleic acid synthesis continues at 45 c, and genomes are segregated along the filament length. when filaments are returned to 30 c, division initiation resumes, and the long multinucleate clones are partitioned into normal-si ...	1972	4628748
[antimicrobial activity of naphthoquinones].		1972	4628766
an exo- -n-acetylglucosaminidase from bacillus subtilis b; extraction and purification.		1972	4628806
on the nature of the radiation damage in the thymine containing strand of hybrid bu-dna after long wave-length u.v.		1972	4628820
uptake of esters of p-hydroxybenzoic acid by serratia marcescens and by fattened and non-fattened cells of bacillus subtilis.		1972	4628829
a compact form of double-stranded dna in solution.		1972	4628857
in vitro repair of x-irradiated dna extracted from bacillus subtilis deficient in polymerase i.	dna extracted from x-irradiated cells of a mutant of bacillus subtilis deficient in dna polymerase i has greatly reduced biological activity. this dna, which has many single-strand nicks, can be repaired in vitro with purified dna polymerase i and dna ligase or with lysates of wild-type b. subtilis cells, which restore biological activity and increase the single-strand molecular weight. lysates of polymerase i-deficient b. subtilis cells cannot repair such irradiated dna until purified dna polym ...	1972	4628873
biosynthesis of polypeptide antibiotics.		1972	4628919
ovine perinatal mortality associated with bacillus cereus.		1972	4628926
[isolation and physicochemical properties of berromycin, a new antineoplastic antibiotic].		1972	4628944
bovine plasma albumin as a supporting matrix in the method of ryter and kellenberger.		1972	4628962
inhibition of a discrete bacterial dna polymerase by 6-(p-hydroxyphenylazo)-uracil and 6-(p-hydroxyphenylazo-)-isocytosine.		1972	4628969
effect of 6-(p-hydroxyphenyl)-azouracil on b. subtilis dna polymerases.		1972	4628970