Publications

[separation of shigella enterotoxin and immunosuppression factors].		1988	3074243
[detection and characterization of collagenolytic activity of serine proteases of the subtilisin type].		1988	3074903
[infantile shigella infections in region ix].		1988	3075065
effect of fermentation of ghanaian maize dough on the survival and proliferation of 4 strains of shigella flexneri.	fermented and non-fermented ghanaian maize dough was seeded with approximately 10(7) colony forming units of 4 strains of shigella flexneri which had been isolated from patients with dysentery. in the non-fermented maize dough (ph 6.2) the shigellae were detectable in large numbers for up to 24 h after exposure. in the maize dough that had been fermented for 3 d (ph 3.2) 3 strains were detectable in small numbers for up to 6 h after inoculation. thereafter none was isolated. the fourth strain, t ...	1988	3076718
antibiotic resistance among shigella species isolated in tehran, iran.	a study of adult patients with gastroenteritis was carried out over an 18-month period from march 1984 until august 1985, with the aim of detecting shigella species and studying their antibiotic resistance pattern. two thousand four hundred and eighteen stool specimens were examined and 230 isolations of shigella were made, a detection rate of 9.5%. of the 230 isolates, 61.2% were found to be sh. flexneri, and resistance to ampicillin, chloramphenicol, co-trimoxazole and tetracycline was extreme ...	1988	3077023
effects of acetyl strophanthidin on duration of atrial fibrillation in the neurally-intact and blockaded dog.	although the inotropic and dromotropic effects of cardiac glycosides in atrial fibrillation (af) are well recognized, their action on af itself is not clear. accordingly, to determine whether cardiac glycosides prolong af, the duration of electrically induced af, atrioventricular conduction, and left ventricular function were assessed for 30 minutes before and for 30 minutes following intravenous administration of acetyl strophanthidin (as), 20 micrograms/kg, in neurally intact, beta-blocked, an ...	1988	3154634
activities of some enzymes of phospholipid metabolism in cultured rat ventricular myocytes in normoxic and hypoxic conditions.	phospholipid catabolism is thought to be one of the critical events in membrane injury during heart ischemia. in this work, the enzymes involved in phospholipid metabolism were studied in purified cultured ventricular myocytes in normoxic and hypoxic conditions. purified ventricular myocytes exhibited an alkaline phospholipase a activity which had sn-2 specificity and which was calcium dependent, and an acid phospholipase a activity with sn-1 specificity. these cells also exhibited lysophospholi ...	1988	3334866
metaplastic breast carcinoma with epithelial phenotype of pseudosarcomatous components.	a case of metaplastic breast carcinoma was subjected to an immunohistochemical study to characterize its apparent fibrosarcomatous and chondrosarcomatous elements. polyclonal and monoclonal antibodies against epithelial cell components such as keratin proteins, epithelial membrane antigen, membrane and cytoplasmic antigens of human mammary carcinoma cells, and carcinoembryonic antigen were used, as well as antibodies against the mesenchymal antigens, desmin, and vimentin. the cells with a mesenc ...	1988	3337621
regulation of central cholecystokinin recognition sites by guanyl nucleotides.	guanyl nucleotides affected the binding of radiolabeled cholecystokinin (cck) octapeptide to rodent cortical binding sites. micromolar quantities of a stable gtp analogue, guanylyl-imidodiphosphate (gppnp), resulted in a plateau where binding was decreased by 30%. in the presence of 25 microm gppnp, binding analysis revealed a decrease in affinity (increase in kd), without an apparent effect on the maximal number of binding sites. ki values for cck-related peptides shifted up to 1.6-fold. the ra ...	1988	3339367
anterior perineal dislocation of the hip with fracture of the femoral head. a case report.	traumatic anterior perineal hip dislocation with an associated fracture of the femoral head is a rare entity. a 26-year-old man injured in a motorcycle accident was treated by closed reduction of the dislocation within three hours after admission. however, several reports of patients with anterior hip dislocation with associated femoral head fractures were treated nonoperatively and had unfavorable results when treatment failed to achieve anatomical position of the fragments. consequently, this ...	1988	3342566
lactic acidosis and recovery of neuronal function following cerebral hypoxia in vitro.	the rat hippocampal slice preparation was used to study the combined effects of hypoxia and lactic acidosis on neuronal function. control slices were exposed to a standard hypoxic insult while being perfused with normal artificial cerebrospinal fluid (acsf). experimental slices were perfused with acsf containing 1.0, 2.0, 10.0 or 20.0 mm lactic acid, 30 min before and during the same standard hypoxic insult. following at 30-min recovery period the ability of these slices to respond to orthodromi ...	1988	3345436
chemosensory anterior dorsal fin in rocklings (gaidropsarus and ciliata, teleostei, gadidae): somatotopic representation of the ramus recurrens facialis as revealed by transganglionic transport of hrp.	the anterior dorsal fin in rocklings consists of a fringe of 50-80 delicate, vibratile rays, which are densely beset with epidermal chemosensory cells. the innervation of these cells is from the dorsal branch of the recurrent facial nerve, which also innervates all other fins and the skin of the trunk. this nerve carries at least three classes of fibres: small (0.5-1.5 micron in diameter), medium (1.5-4 micron), and large (greater than 4 micron). approximately 12,000 small and weakly myelinated ...	1988	3346379
differences in synaptic inputs to preganglionic neurons in the dorsal and lateral band subdivisions of the cat sacral parasympathetic nucleus.	in the cat, preganglionic neurons (pgns) found in the dorsal portion of the sacral parasympathetic nucleus (dorsal band or db cells) participate in bowel control, while those found along the lateral edge (lateral band or lb cells) influence bladder function. in order to determine whether differences in the synaptic inputs exist between these two populations, hrp was applied to the sacral ventral rootlets of cats, and the s2 cord segment was prepared for sequential light and electron microscopy. ...	1988	3346386
corticotropin-induced changes in enzymatic activities of the post-pregnenolone steroidogenic pathway in rabbit adrenocortical cells.	in an attempt to delineate the effect of corticotropin (acth) on post-pregnenolone steroidogenesis, the activity of enzymatic systems operative in conversion of pregnenolone into glucocorticoids and androgens was studied in adrenocortical cells from control rabbits and from animals treated with acth for 12 days (acth 1-24, 200 micrograms s.c. daily). the cells from acth-treated rabbits exhibited an increased overall steroidogenic capacity and produced much more cortisol (p less than 0.0005) as w ...	1988	3347065
[meningoencephalitis and mononucleosis syndrome associated with acute infection by human immunodeficiency virus].		1988	3347109
functionally illiterate worker also has 'right to understand'.		1988	3347451
the duck hepatitis b virus dna polymerase is tightly associated with the viral core structure and unable to switch to an exogenous template.	the duck hepatitis b virus (dhbv) has a dna polymerase associated with it which uses the incomplete viral genome as endogenous template. a prerequisite for studying this polymerase is the availability of conditions to open viral cores without destroying their enzymatic activity. in this study, this was achieved by a brief treatment with low ph. dhbv dna in low-ph-treated cores was susceptible to digestion with deoxyribonuclease i and restriction enzymes, and large restriction fragments diffused ...	1988	3347995
susceptibilities of hong kong isolates of multiply resistant shigella spp. to 25 antimicrobial agents, including ampicillin plus sulbactam and new 4-quinolones.	seventy-two percent of 129 shigella isolates from two hong kong hospitals were shigella flexneri. twenty-six percent were s. sonnei, and there was only one isolate each of s. dysenteriae and s. boydii. ninety-six percent of the isolates were resistant to two or more antibiotics, and up to 11 resistances were seen in a single isolate. fifty-seven percent or more of these isolates were resistant to ampicillin, streptomycin, tetracycline, chloramphenicol, and sulfamethoxazole; and up to twenty-thre ...	1988	3348608
[amino acid composition of the lymph and blood in fever reactions of different durations].	the amino acid composition of the thoracic duct lymph and blood following fever reactions (fr) of various duration has been studied in the experiments on rabbits. the experiments have shown that some changes in protein metabolism in fr are primarily manifested in amino acid lymph composition. irrespective of fr duration the amino acid content in the lymph almost always increases, which confirms the opinion concerning catabolic process predominance in protein metabolism during fr. the lack of "cu ...	1988	3349141
anti-inflammatory activity of oleoresin from brazilian copaifera.	the oleoresin from brazilian copaifera species yielded copalic acid and sesquiterpenes and showed marked anti-inflammatory activity using various experimental models in rats. the oleoresin significantly inhibited carrageenin-induced pedal edema following oral doses from 0.70 to 2.69 ml/kg, but was somewhat less effective than 50 mg/kg calcium phenylbutazone. repeated administration of the oleoresin at a dose of 1.26 ml/kg for a 6-day period reduced granuloma formation with a response comparable ...	1988	3352280
[tobacco or health: choose health].		1988	3352398
chorioamnionitis: a study of organisms isolated in perinatal autopsies.	the purpose of this study was to determine the validity of postmortem cultures in perinatal autopsies; in particular, those cases where there is gross or histologic evidence of chorioamnionitis. lung, liver, blood, and placental cultures were obtained from 159 neonatal autopsies with histologic evidence of chorioamnionitis at magee-women's hospital between january 1980 and july 1985. the criterion for chorioamnionitis was a polymorphonuclear leukocytic infiltrate in the placental membranes. the ...	1988	3355095
septal thickness in human lungs assessed by computerized interactive morphometry.	we have studied the feasibility of using interactive computerized morphometric methods for the quantitation of septal thickness in the lung with the goal of introducing objective criteria for the diagnosis and grading of pulmonary fibrosis and edema. after reviewing available methods, we have decided in favor of linear intercept measurements of septal profiles which are limited to the tissue and therefore not affected by the level of lung inflation. our method consisted in generating a graphic o ...	1988	3357335
responsiveness of genetically epilepsy-prone rats to intracerebroventricular morphine-induced convulsions.	the sensitivity to intracerebroventricular morphine-induced convulsions was determined in members of the severe seizure (gepr-9) and moderate seizure (gepr-3) colonies of genetically epilepsy-prone rats as well as in non-epileptic control rats. gepr-9s were more sensitive to morphine-induced wet-dog shakes, rearing with bilateral forelimb clonus and generalized clonus than controls of gepr-3s. gepr-3s were less sensitive to morphine-induced wet-dog shakes and rearing with bilateral forelimb clon ...	1988	3362037
effects of lesions of the dorsal noradrenergic bundle on successive discrimination in the rat.	male sprague-dawley rats were trained to bar-press for food reward on a successive discrimination involving periods of reward on a variable-interval (vi) 18-s schedule interspersed with periods of extinction. the two components of the schedule were signaled by a steady or a flashing light, counterbalanced between vi and extinction components. confirming previous findings, the discrimination was easier when the flashing light signaled vi and the steady light signaled extinction, than with the rev ...	1988	3365184
n-acetylcysteine inhibits human neutrophil and monocyte chemotaxis and oxidative metabolism.	the effect of n-acetylcysteine (nac) on human neutrophil and monocyte cell viability, chemotaxis, oxygen consumption and chemiluminescence was studied. it was found that nac at concentrations higher than 3 x 10(-2) m resulted in neutrophil and monocyte cytotoxicity. the studies on the effect of nac on neutrophil and monocyte chemotaxis showed that nac inhibited chemotaxis of both cell types in a concentration dependent manner. nac at 3 x 10(-2) m inhibited chemotaxis of both cell types by about ...	1988	3366508
a comparison of the effects of measured, predicted, estimated and constant residual volumes on the body density of female athletes.	the body density (bd), and hence the relative body fat (% bf) was measured for 182 female athletes. the residual volume (rv) was determined both before and after the underwater weighing by a multiple breath helium dilution technique with the subject immersed to neck level. the absolute mean difference (lxdl) and see between the two rv trials were 63 and 75 ml, respectively. these increased to values ranging 144-685 and 187-252 ml, respectively, when the mean of the two rv trials for each subject ...	1988	3366515
antimicrobial susceptibility testing of streptococcus pneumoniae: quality assessment results.	six strains of streptococcus pneumoniae were distributed to 405 united kingdom laboratories who were asked to test the susceptibility of the strains to penicillin, tetracycline, chloramphenicol and erythromycin and to provide details of methodology to test the standards of susceptibility testing. high error rates were seen only in failure to detect moderate resistance to penicillin (12%) and resistance to chloramphenicol (16%). increased error rates were associated with several methods or practi ...	1988	3366921
[effect of prolactin on the enzymatic activity of thyroid follicular cells during stress due to surgery and subsequent subcutaneous inflammation].	in order to reveal prolactin (prl) effect on the enzymatic activity of the thyroid follicular cells at a subcutaneous inflammation, 2 series of experiments have been performed on 100 non-inbred male rats with body mass of 150-170 g. in order to produce the inflammation, under ether narcosis celloidin globules are introduced subcutaneously in the abdominal wall. the test animals are given 0.125 mg of "lactin" (the soviet preparation of bovine prl) in 0.5 ml of isotonic solution once every day, be ...	1988	3369954
[effect of caldesmon on the type of conformation changes in f-actin induced by the binding of myosin 1 subfragment].	the effect of caldesmon on the conformational changes of f-actin caused by myosin subfragment 1 (s-1) binding was studied, using the polarized microfluorimetry method. it was demonstrated that the polarized fluorescence of rhodaminil-phalloin specifically bound to f-actin of pure actin filaments as well as of tropomyosin-containing actin filaments changes as a result of binding to s-1. the nature of these changes depends on the presence of caldesmon in the filaments. caldesmon was supposed to mo ...	1988	3370245
increased hepatic amino nitrogen conversion after elective cholecystectomy in man.	1. the effect of elective, uncomplicated cholecystectomy on plasma clearances of amino acids and on amino acid-stimulated urea synthesis was investigated in 10 patients, pre-operatively and on the first post-operative day, and compared with six controls treated identically apart from the surgery. 2. a mixture of amino acids was given as a prime-continuous infusion. steady-state concentrations 75% higher than basal were attained and were maintained for 90 min. the clearances of amino acids were c ...	1988	3370921
characterization of size-dependent exchange of peg molecules between the blood and extravascular space in the pig.	different sized polyethylene glycols (pegs) have been used as probe molecules in studies of size-dependent permeation through the intestinal wall and the glomerular membranes. we have curve-fitted a three-compartment model to the urinary recovery data following intravenous injection of different sized peg molecules in the pig. the rate constants to and from the extravascular space demonstrate a strong size-dependent selectivity for peg molecules less than 502 da, but the rate constants to the ur ...	1988	3370951
trichosanthin, alpha-momorcharin and beta-momorcharin: identity of abortifacient and ribosome-inactivating proteins.	the abortifacient proteins trichosanthin, alpha-momorcharin and beta-momorcharin at nm concentrations inhibit cell-free protein synthesis. the momorcharins and the ribosome-inactivating proteins isolated from momordica charantia seeds cross-react with the respective antisera. the ribosome-inactivating proteins saporins, pokeweed antiviral protein (pap) and, to a lesser extent, gelonin have abortifacient activity on pregnant mice.	1988	3372132
ocular toxoplasmosis in immunosuppressed nonhuman primates.	to investigate the role of cellular immunodeficiency in recurrent toxoplasmic retinochoroiditis, six cynomolgus monkeys (macaca fascicularis) with healed toxoplasmic lesions of the retina were immunosuppressed by total lymphoid irradiation. three months prior to irradiation 30,000 toxoplasma gondii organisms of the beverley strain had been inoculated onto the macula of eye in each monkey via a pars plana approach. toxoplasmic retinochoroiditis developed in each animal, and lesions were allowed t ...	1988	3372160
anticonvulsant effects of dextrorphan in rats: possible involvement in dextromethorphan-induced seizure protection.	the major metabolite of the non-opioid anticonvulsant/antitussive dextromethorphan is dextrorphan. in the present study, the effects of dextrorphan were determined in an experimental model of seizure activity (maximal electroshock convulsions) (mes). subcutaneous administration of dextrorphan produced dose-related blockade of tonic hindlimb extension (the) and a decrease in the duration of tonic forelimb extension (tfe). the anticonvulsant effect of dextrorphan was linear and maximally efficacio ...	1988	3374269
fiberoptic angioscopy of cardiac chambers, valves, and great vessels using a guiding balloon catheter in dogs.	the applicability of fiberoptic angioscopy with a guiding balloon catheter to observe the cardiac chambers, valves and the great vessels was examined in anesthetized dogs. a no. 11 french guiding balloon catheter (balloon diameter 50 french) was introduced through either the right jugular vein or the right femoral vein into the right heart, and then a fiberscope (4.3 french in diameter) was introduced through the catheter into the right heart in 10 dogs. the balloon was inflated with air and gen ...	1988	3376848
[acute hepatitis: prevention and treatment].		1988	3377335
electrophysiological effects of cocaine and procaine on dorsal raphe serotonin neurons.	the effects of cocaine and procaine on the spontaneous activity of serotonin (5-hydroxytryptamine, 5-ht) neurons in the dorsal raphe nucleus of the chloral hydrate anesthetized male rat were characterized using extracellular single-unit and microiontophoretic recording techniques. intravenous cocaine elicited a potent, dose-dependent inhibition of 5-ht cell firing with a cumulative dose to 50% inhibition of cell firing of 0.66 +/- 0.11 mg/kg (means +/- s.e.m.; n = 17). in contrast, systemic admi ...	1988	3384007
antibody response of an elderly population to a supplemental dose of influenza b vaccine.	recent reports have suggested that the antibody response of elderly persons to standard doses of influenza vaccine is depressed. we examined the effect of an additional threefold dose of influenza b vaccine on the antibody response in elderly, ambulatory veterans. one hundred thirty-one male subjects aged 70 years and older were randomized to receive one of three influenza vaccine regimens: group i received standard trivalent influenza vaccine containing 15 micrograms of b/ussr/100/83 in one arm ...	1988	3385111
[study of the effect of antitumor agents on cell cycle traverse by flow cytometry].	the effect of antitumor agents on the cell cycle traverse of cultured fl cells, a monolayer culture line derived from human amnion, was investigated by flow cytometry (fcm) in combination with simultaneous determination of total cell number, viability, mitotic index and labeling index by [3h]tdr. the results obtained can be summarized as follows: antimetabolites prolonged the duration of the s phase at a low dose, while at a high dose they decreased the rate of progression from the g1 to s phase ...	1988	3389824
taurine prevention of calcium paradox-related damage in cardiac muscle. its regulatory action on intracellular cation contents.	the present study was designed to investigate in chick heart whether oral pretreatment with taurine or taurine added directly to the perfusate has any effect upon calcium paradox-induced heart failure. in both protocols, taurine significantly reduced the mechanical dysfunction resulting from the calcium paradox. taurine pretreatment partially inhibited the excess accumulation of calcium in the myocardium that occurs upon calcium repletion, and microscopy revealed almost normal structure. this pr ...	1988	3390225
cadmium-substituted skeletal troponin c metal binding investigations and sequence assignment of the cadmium-113 resonances.	the binding of cadmium to the calcium binding subunit of skeletal troponin (stnc) has been reinvestigated using direct binding methods and fluorescent derivatives. these data provide straightforward explanations of the observed titration behavior in the 113cd nmr (ellis, p.d., strang, p., and potter, j.d. (1984) j. biol. chem. 259, 10348-10356). further, fluorescent derivatives of skeletal troponin c provide an excellent means of establishing a sequence assignment for the resonances observed in ...	1988	3392014
[the differentiation of bordetella bronchiseptica strains. 7. phenotypic changes in bordetella bronchiseptica through changes of phase].		1988	3395180
representation of forelimb mechanoreceptors within the superior colliculus of the cat.	forelimb mechanoreceptor representation within the superior colliculus was investigated using microelectrode recording in decerebrate or anaesthetized cats. selective activation of different mechanoreceptive fibre classes supplying the distal forelimb revealed a poor representation of all three fibre classes (sa, pc and ra) that innervate the glabrous skin. furthermore, there was no evidence for pacinian (pc) input from the interosseous or wrist region. the tap stimuli which are often needed to ...	1988	3399131
pharmacokinetics and pharmacodynamics of single oral doses of ibopamine, quinidine and their combination in normal man.	the pharmacokinetics and haemodynamics (phono- and impedance cardiography) of single oral doses of 200 mg ibopamine (sk&f 100168), 400 mg quinidine sulphate, and their combination, have been assessed in 6 healthy male volunteers. no significant differences in the mean pharmacokinetic parameters of either drug were seen between the single and combined doses. ibopamine caused an increase in mean estimated stroke volume (sv +29% for the maximum change from baseline and +15% cumulatively over the fi ...	1988	3402527
expression of fibronectin on clonally related transformed and control sublines from an epithelial cell strain and a tumor line of mouse alveolus.	the cellular expression of fibronectin and the importance of fibronectin for the morphology of cultured cells were studied in a lung epithelial cell system. the cells used were clonally related control nal1a, spontaneously transformed nal1as cells of the nal1a cell strain, and transformed clonal sublines of the cell line nal1am and the tumor cell line nul1. fibronectin was detected on the surface of nal1a cells by surface iodination; fibronectin synthesis, secretion, and pericellular accumulatio ...	1988	3409226
a new constrictor device for external induction of a long-term stable and irreversible renal artery stenosis in the dog.	a new externally adjustable constrictor device for renal artery stenosis in the dog is described. the constrictor combines hydraulic and mechanical characteristics, and is connected to the exterior of the animal by a thin catheter. applying hydraulic pressure via the catheter causes the plunger in the device to compress the renal artery to any desired degree of stenosis. a mechanical catch prevents backward movement of the plunger, thus ensuring a stable, irreversible renal artery constriction. ...	1988	3412870
production of a positive direct antiglobulin test due to suramin.	drugs and chemicals containing multiple reactive carbonyl groups have been postulated to produce nonimmunologic positive direct antiglobulin tests (dats) both in vitro and in vivo. suramin sodium, a reverse transcriptase inhibitor that was under investigation as a potential treatment for human immunodeficiency virus infection, has six reactive carbonyl groups. because of suramin's chemical structure, it was hypothesized that the drug might cause a positive dat in vitro and possibly in vivo. sura ...	1988	3415441
[dynamics of folliculogenesis in the mouse ovary after the intrauterine elimination of germ cells by administering cyclophosphamide].	injection of cytostatic--cyclophosphamide--to pregnant mice produces elimination of germ cells in their offspring ovaries. independently on the elimination degree of the germ cells, the amount of growing follicles, as well as dynamics of their growth do not differ from those in the majority of control (intact) animals. in some cases the delay in the rate of the follicular growth is connected with regional outgrowth of the interstitial tissue. this, in its turn, depends on uneven distribution of ...	1988	3415486
metabolism of acetylene and acetaldehyde by rhodococcus rhodochrous.	we studied the ability of a soil bacterium, identified as rhodococcus rhodochrous, to grow on acetylene and to accumulate acetaldehyde. its maximum growth rate on acetylene was obtained at about 30 degrees c (mu = 0.11 h-1) and was independent of the concentration of this gas in air from 0.14 to 16% (v/v). during growth, acetylene was quantitatively transformed to acetaldehyde, ethanol, acetate, co2, and biomass in proportions which varied with culture age and temperature. growth was completely ...	1988	3416237
immunosuppressive substance in the sera of head and neck cancer patients.	serum levels of immunosuppressive substance (is) and immunosuppressive acidic protein (iap) were studied by single radial immunodiffusion in 108 patients with head and neck cancer. mean values of serum is and iap in patients with head and neck cancers were as follows: laryngeal carcinoma, 955 +/- 447 micrograms/ml (is), 604 +/- 324 micrograms/ml (iap); maxillary carcinoma, 896 +/- 384 micrograms/ml (is), 568 +/- 246 micrograms/ml (iap); nasopharyngeal carcinoma, 871 +/- 313 micrograms/ml (is), 6 ...	1988	3416271
the regional variability of enzymes in the brain: relevance to csf enzyme determinations.	the assay of cerebrospinal fluid (csf) enzymes has been suggested for assessing the extent of damage and patient prognosis in cases of brain injury. a potential difficulty associated with using csf enzyme levels as predictors of outcome is the possibility that enzyme concentrations may vary substantially from one brain region to another. we have determined the concentrations of seven enzymes in seven brain regions in the rat and cat. acid phosphatase (acp), aspartate aminotransferase (ast), isoc ...	1988	3416484
experimental contact sensitization with 3,4,5-trichloropyridazine.	the potential of 3,4,5-trichloropyridazine to induce contact sensitization was assessed in the guinea pig maximization test of magnusson and kligman and also in the closed patch test described by buehler. the test material was a 1% solution of 3,4,5-trichloropyridazine in a highly refined mineral oil. the test material elicited moderate to severe irritation when diluted in mineral oil to concentrations of 15-25% and minimal irritation at concentrations of 1-3%. both tests clearly indicated that ...	1988	3416588
effect of retinoids on the proliferation, morphology and expression of glial fibrillary acidic protein of an anaplastic astrocytoma cell line.	we studied the effect of retinoids on the growth and differentiation of a cell line (u 343 mg-a) derived from a human malignant astrocytoma. cultures treated with all-trans or 13-cis retinoic acid showed a dose-dependent inhibition of proliferation and a marked reduction in the mean cell number at the plateau phase of growth (3.5 x 10(6) vs. 1 x 10(7) cells/25 cm2) compared with untreated cultures. at confluence, cells treated with all-trans or 13-cis retinoic acid were contact-inhibited, wherea ...	1988	3417370
effect of gamma irradiation on the protoscoleces of echinococcus granulosus of sheep origin.		1988	3417384
internal pars plana sclerotomy with the contact nd:yag laser: an experimental study.	transvitreal pars plana sclerotomy using the contact nd:yag laser with a sapphire probe was performed on a group of rabbit eyes and compared with a similar procedure performed with a ziegler knife. although the intraocular pressure (iop) was lowered in both groups of animals, the reduction was greater and more lasting in the group on which the nd:yag laser with sapphire probe was used. this technique may have application in cases with extensive peripheral anterior synechiae or where a vitrectomy ...	1988	3417390
optic disc, cup and neuroretinal rim size, configuration and correlations in normal eyes.	four hundred and fifty-seven unselected normal human optic nerve heads of 319 subjects (163 men, 156 women, mean age 42.7 +/- 19.6 years) were evaluated by magnification-corrected morphometry of optic disc photographs. mean optic disc surface measured 2.69 +/- 0.70 mm2 (0.80-5.54 mm2), mean diameter horizontally 1.76 +/- 0.31 mm (0.91-2.61 mm), and vertically 1.92 +/- 0.29 mm (0.96-2.91 mm). the form was slightly vertically oval. optic cup area averaged 0.72 +/- 0.70 (0.00-3.41 mm2), mean horizo ...	1988	3417404
hypotheses on exercise-induced pulmonary hemorrhage in horses.		1988	3417533
crystallization of trimeric recombinant human tumor necrosis factor (cachectin).	crystals of tumor necrosis factor (tnf) have been obtained in two forms. rhombohedral crystals grow in 1.8 to 2.0 m ammonium sulfite, ph 7.8 at 21 degrees c, and tetragonal crystals grow in 2.6 m magnesium sulfate, ph 5.5 at 25 degrees c. analysis of tnf by isoelectric focusing under native and denaturing conditions indicates that tnf molecules exist as trimers in solution. the rhombohedral cachectin crystals belong to space group r3 and have unit cell constants a = b = c = 47.65 a and alpha = b ...	1988	3417634
the fate of venous repair after shock and trauma.	to assess the role of shock and trauma in the subsequent thrombosis of a venous repair, the following experiments were performed. two groups of unsplenectomized dogs were studied: control dogs (gr.c) underwent replacement of a segment of superficial femoral vein by an autogenous vein graft. trauma group (gr.t) dogs were subjected to hypovolemic shock using a modified wiggers' technique and received standardized hindleg trauma. after resuscitation, venous repairs identical to gr.c dogs were perfo ...	1988	3418758
pharmacokinetics of bupivacaine after short and prolonged infusions in conscious dogs.	a conscious dog model was used to study pharmacokinetics of bupivacaine after a short infusion (si) (15 min) and a prolonged infusion (pi) (24 hours). bupivacaine was infused in six mongrel dogs at least 10 days after implantation of femoral arterial and venous catheters. each dog received both the si and the pi in a random crossover design at a one week interval. bupivacaine concentration was measured in serum sampled during the si, during the last hour of the pi, and at frequent intervals duri ...	1988	3421499
experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination.	the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ...	1988	3421507
native genomic blotting: high-resolution mapping of dnase i-hypersensitive sites and protein-dna interactions.	dnase i-hypersensitive sites are observed in the promoter regions of actively expressed genes, potentially active genes, and genes that were once active. we have developed an approach that greatly increases the resolution for mapping these sites by electrophoresing genomic dna on native polyacrylamide gels prior to electroblotting and hybridization. this improved method has been used to scan the promoter and coding region of a cell-cycle-dependent human histone h4 gene with an accuracy of +/-5-1 ...	1988	3422413
ketone-body metabolism in hyperthyroid rats: reduced activity of d-3-hydroxybutyrate dehydrogenase in both liver and heart and of succinyl-coenzyme a: 3-oxoacid coenzyme a-transferase in heart.	the specific activity of d-3-hydroxybutyrate dehydrogenase is reduced by about a third in liver and heart mitochondria of hyperthyroid rats. state 3 respiration is also reduced in isolated mitochondria from the same animals when dl-3-hydroxybutyrate is the substrate. determination of the kinetic parameters of the membrane-bound d-3-hydroxybutyrate dehydrogenase in liver of hyperthyroid rats reveals a decreased in maximal velocity (vmax). the michaelis and dissociation constants of nad+ and d-3-h ...	1988	3422549
analysis and isolation of cytomegalovirus dna by field inversion gel electrophoresis.	high molecular weight human cytomegalovirus (cmv) dna was isolated from agarose-embedded infected human diploid cells by employing field inversion gel electrophoresis. a high yield of cmv dna molecules was obtained within 1 week of infecting the cell culture. labelling of the viral dna with biotin by nick translation enabled the detection of cmv-infected cells in sections of paraffin-embedded human adrenal gland by in situ hybridization.	1988	2832532
analysis and isolation of cytomegalovirus dna by field inversion gel electrophoresis.	high molecular weight human cytomegalovirus (cmv) dna was isolated from agarose-embedded infected human diploid cells by employing field inversion gel electrophoresis. a high yield of cmv dna molecules was obtained within 1 week of infecting the cell culture. labelling of the viral dna with biotin by nick translation enabled the detection of cmv-infected cells in sections of paraffin-embedded human adrenal gland by in situ hybridization.	1988	2832532
viruses in sewage waters during and after a poliomyelitis outbreak and subsequent nationwide oral poliovirus vaccination campaign in finland.	during an outbreak of paralytic poliomyelitis in finland in 1984 and 1985 the widespread circulation of the causative wild-type serotype 3 poliovirus in the population was documented by demonstrating the virus in sewage water specimens in 13 different locations in the greater helsinki district and in 13 other cities or towns all over the country. after the nationwide campaign with oral poliovirus vaccine in 1985, poliovirus serotypes 2 and 3 were readily isolated from sewage waters for up to 2 m ...	1988	2833160
monoclonal antibodies with neutralizing activity segregate isolates of bovine viral diarrhea virus into groups. brief report.	isolates of bovine viral diarrhea (bvd) virus were differentiated by monoclonal antibodies (moabs) reactive with the 56 kd viral polypeptide. patterns of neutralizing activity of the moabs indicate that multiple epitopes are involved in virus neutralization.	1988	2833200
inhibition of allergic encephalomyelitis by the iron chelating agent desferrioxamine: differential effect depending on type of sensitizing encephalitogen.	induction of experimental allergic encephalomyelitis (eae) in lewis rats by injection of guinea pig (gp) spinal cord homogenate (sch) plus adjuvant (sch-cfa) can be inhibited by treatment with the iron chelating agent desferrioxamine (dfom). interestingly, induction of eae with purified myelin basic protein (bp-cfa) is not inhibited with dfom. this dichotomy does not appear to be due to any quantitative differences in the two inocula since minimal clinical eae produced by threshold levels of bp ...	1988	2447124
shiga toxin inhibits the anti-invasive effect of interferons.		1988	2450154
[relation of the immunosuppressivity of virulent shigella to the structure of o antigen].	the capacity of filtrates of virulent shigella cultures (vsc) and their different fractions to render avirulent strains, injected intraperitoneally into mice simultaneously with these filtrates, capable of suppressing immune response (delayed hypersensitivity) has been studied. among the fractions of vsc filtrates, the lipoid fraction soluble in the mixture of ethanol and ether has proved to be active. active factors can be extracted from vsc filtrates by means of immunosorbents obtained from an ...	1988	2452538
high-yield purification of a pp60c-src related protein-tyrosine kinase from human platelets.	a protein-tyrosine kinase (ptk, ec 2.7.1.112) from human platelets was purified with high yield. purification of the enzyme involved sequential chromatography on casein-agarose, tyrosine-agarose, heparin-sepharose and hydroxylapatite. the procedure resulted in substantially enriched 54/52 kda polypeptides on sds-polyacrylamide gel electrophoresis and a yield of about 25% in ptk activity. about 250 micrograms of purified protein could be obtained from 1 g of cell protein. the purification factor ...	1988	2453218
natural killer cell activation and interferon production by peripheral blood lymphocytes after exposure to bacteria.	we have previously shown that peripheral blood natural killer (nk) cells have significant levels of cytotoxic activity against shigella flexneri-infected hela cells. in this report, we show that nk cell activity against k562 tumor cells and shigella flexneri-infected hela cells can be greatly enhanced by preincubating peripheral blood lymphocytes (pbl) for 18 h with kanamycin-treated shigella flexneri or salmonella typhimurium. cell-free supernatants obtained from pbl-bacteria cultures contained ...	1988	2453465
activation of the mas oncogene during transfection of monoblastic cell line dna.	using the tumorigenicity assay, we observed an activation of the mas oncogene during transfection of human acute myelocytic leukemia (ctv-2) dna. respective transfectants contained amplified mas sequences characterized by rearrangements in 5' and 3' noncoding regions that were transcribed into a 3.3 kb mas rna species. our data represent the second report on activation of the mas gene.	1988	2453759
structural and immunochemical studies of the lipopolysaccharide from a new provisional serotype of shigella flexneri.	the chemical structure of the o-antigen of a proposed new provisional serotype of shigella flexneri has been determined. methylation analysis, glc-ms, 1h-nmr and 13c-nmr showed that the linear o-antigenic polysaccharide is the same as for all s. flexneri [kenne, l., lindberg, b., petersson, k. & romanowska, e. (1977) carbohydr. res. 56, 363-370]. a novel structural feature is that the disaccharide alpha-d-glcp-(1----2)-alpha-d-glcp is linked to o4 of the n-acetyl-glucosamine residue. (formula: s ...	1988	2458260
shigella flexneri invasion plasmid antigens b and c: epitope location and characterization with monoclonal antibodies.	invasion plasmid antigens b (ipab) and c (ipac) are associated with the ability of shigellae to invade cultured mammalian cells. monoclonal antibodies against ipab and ipac polypeptides were produced and used in a whole-cell enzyme-linked immunosorbent assay to show that both ipab and ipac polypeptides were exposed on the surface of virulent shigellae. moreover, these surface epitopes were shown to be highly conserved among different serotypes of shigella spp. and enteroinvasive escherichia coli ...	1988	2459066
bacterial epitopes involved in the induction of reactive arthritis.	a panel of documented arthritogenic shigella flexneri strains as well as an epidemic-associated non-arthritogenic shigella sonnei control was used to identify a 2-megadalton plasmid specific to the arthritogenic strains. the plasmid, phs-2, contains a dna sequence that encodes a 22-amino acid polypeptide encompassing a pentapeptide homologous to part of the polymorphic region of the alpha-1 domain of hla-b27. these results suggest that molecular mimicry between arthritogenic bacterial-encoded ep ...	1988	2462352
development and testing of invasion-associated dna probes for detection of shigella spp. and enteroinvasive escherichia coli.	genetic determinants of the invasive phenotype of shigella spp. and enteroinvasive escherichia coli (eiec), two common agents of bacillary dysentery, are encoded on large (180- to 210 kilobase), nonconjugative plasmids. several plasmid-encoded antigens have been implicated as important bacterial ligands that mediate the attachment and invasion of colonic epithelial cells by the bacteria. selected invasion plasmid antigen (ipa) genes have recently been cloned from shigella flexneri serotype 5 int ...	1988	2830310
three distinct forms of type 2a protein phosphatase in human erythrocyte cytosol.	two type 2a protein phosphatases, phosphatases i (mr = 180,000) and iii (mr = 177,000), were purified to near homogeneity from human erythrocyte cytosol. phosphatase i was composed of alpha (34 kda), beta (63 kda), and delta (74 kda) subunits in a ratio of 1:1:1. phosphatase iii comprised alpha, beta, and gamma (53 kda) subunits in the same ratio. heparin-sepharose column chromatography converted most of phosphatase i and 20% of phosphatase iii into alpha 1 beta 1 which were indistinguishable fr ...	1988	2831201
apolipoprotein e-rich hdl binding to liver plasma membranes in copper-deficient rats.	copper deficiency in rats raises plasma cholesterol concentration while reducing live cholesterol concentration. one consequence of this cholesterol redistribution is the accumulation of a large high-density lipoprotein (hdl) particle rich in apolipoprotein e (apo e). the purpose of this study was to determine, using an in vitro binding assay, if the interaction of apo e-rich hdl with hepatic lipoprotein binding sites may be affected by copper deficiency. male sprague-dawley rats were divided in ...	1988	2831541
feeding by mucin and intestinal growth of some enteric bacterial pathogens.	enteroinvasive escherichia coli, salmonella typhi-murium, shigella sonnei, shigella flexneri, as well as e. coli k-12 show dose dependent growth in minimal medium completed with purified hog gastric "granular mucin". this ability is based on alpha-galactosidase production: defective, melibiose (and galactose) non-fermenting k-12 mutant were unable to utilize mucin. the viability of the parent k-12 strain in the cecal content of mice is significantly higher than that of its mel- mutant phenotype. ...	1988	2469280
interaction between bambuterol and physostigmine: aspects on cholinesterase inhibition and neuromuscular transmission in the smooth and skeletal muscles of the guinea-pig.	bambuterol, the bis-dimethyl carbamate prodrug of terbutaline, and physostigmine were examined with respect to their ability to interfere with the neuromuscular transmission in an isolated vagus nerve-trachea preparation, a phrenic nerve-diaphragm preparation and the transmurally stimulated extensor digotorum longus (edl) isolated from the guinea-pig. physostigmine increased the contractile response of the trachea to stimulation of the vagus nerve. bambuterol had an opposite effect in this respe ...	1988	2848230
comparisons of the pathogenicity of long and short fibres of chrysotile asbestos in rats.	long-term inhalation and intraperitoneal injection studies were undertaken with laboratory rats treated with a specially prepared short-fibre sample of canadian chrysotile asbestos. this was compared, at an equal mass dose, to dust generated from the same chrysotile batch so as to contain the highest possible number of long fibres. the long-fibre cloud contained roughly five times more fibres greater than 5 micron in length as seen by phase contrast optical microscopy (pcom). for increasing leng ...	1988	2848570
expression of env sequences of the bovine leukemia virus (blv) in the yeast saccharomyces cerevisiae.	dna sequences of the envelope (env) gene of the bovine leukemia virus (blv) were expressed in the yeast saccharomyces cerevisiae. two yeast promoters, the repressible pho5 promoter and the constitutive pgk promoter, were used to construct four expression plasmids comprising either a sequence of the surface antigen gp51 or a (gp51 + gp30) sequence. the expressed heterologous gene products were characterized by western blot analysis and competitive radioimmunoassay. by means of northern blot analy ...	1988	2849260
peripheral neuropathy in elderly cba mice.	a late-onset peripheral neuropathy in a mouse with a wider progressive neurological disorder is morphologically characterized for comparison with late life human neuropathies. preliminary studies indicate the presence of a proximal, predominantly motor, axonopathy in the mouse model.	1988	2849396
salience and the effects of cs preexposure on aversion conditioning.	rats (n = 84) received preexposure to distilled water or to one of two differently salient flavors, 5.0% casein or 10.0% sucrose, casein being the more salient. each preexposure group then received aversion conditioning to a 5.0% casein or a 10.0% sucrose cs. aversion effects were reliably more enduring to casein than to sucrose. relative to water-preexposed groups, preexposure to casein attenuated aversion effects to the casein cs reliably less than preexposure to sucrose attenuated aversion ef ...	1988	2849411
similarity between human retinoic acid receptor and escherichia coli homoserine kinase.		1988	2849427
a new class of free radical scavengers reducing adriamycin mitochondrial toxicity.	beef heart mitochondria were incubated with adm and nadh. an adriamycin semiquinone radical was detected using esr spectroscopy. the semiquinone radical production rate is decreased upon addition of a scavenger (ad 20) in the reaction medium. nmri mice were treated with ad 20 (70 mg/kg, i.p.) 15 min prior adm injection (20 mg/kg, i.p.) or with adm alone. heart mitochondria were isolated 48 hr later. the enzymatic activities of complex i-iii and complex iv of the mitochondrial respiratory chain w ...	1988	2849451
the cupric site in nitrous oxide reductase contains a mixed-valence [cu(ii),cu(i)] binuclear center: a multifrequency electron paramagnetic resonance investigation.	multifrequency electron paramagnetic resonance (epr) spectra of the cu(ii) site in nitrous oxide reductase (n2or) from pseudomonas stutzeri confirm the assignment of the low field g value at 2.18 consistent with the seven line pattern observed at 9.31 ghz, 10 k. s-band spectra at 20 k are better resolved than the x-band spectra recorded at 10 k. the features observed at 2.4, 3.4, 9.31 and 35 ghz are explained by a mixed-valence [cu(1.5)..cu(1.5)] s = 1/2 species with the unpaired electron deloca ...	1988	2849565
human rotavirus infection enhances invasiveness of enterobacteria in ma-104 cells.	to study the interaction between common pathogens causing infectious diarrhea in humans, ma 104 cell cultures were infected with human rotavirus and salmonella typhimurium or shigella flexneri or enteroinvasive escherichia coli (eiec) concomitantly. when ma-104 cells were preinfected with human rotavirus, invasiveness of s. typhimurium was significantly enhanced. the enhancement was evident after 48 h of virus preincubation. at this time virus specific antigens were demonstrated in the cell cult ...	1988	2850823
endotoxin-induced uveitis in rats: morphological and biochemical study.	inflammation induced by systemic injection of endotoxin can be a good model for endogenous uveitis since ocular inflammation is induced without manipulating the eye. we carried out morphological and biochemical studies in lewis rats to evaluate the breakdown of the blood-ocular barrier following injection of endotoxin (1 mg/rat) in footpads. vasodilation was observed as early as 3 hours and became maximum at 18-24 hours after the injection. unlike in the eye, no inflammatory changes were observe ...	1988	2853240
in vitro activity of azithromycin (cp-62,993), a novel macrolide, against enteric pathogens.	azithromycin (cp-62,993 [9-deoxy-9a-methyl-9a-aza-9a-homoerythromycin]) is a novel macrolide antimicrobial. in this study the in vitro activity of cp-62,993 has been determined against selected enteropathogens, including clostridium difficile, and compared with that of erythromycin. mics were determined using an agar incorporation technique in mueller-hinton medium, containing saponin-lysed horse blood at a final concentration of 10% v/v, with an inoculum of 10(4) cfu. cp-62,993 was considerably ...	1988	2854515
selective induction of chromosomal gene expression by human cytomegalovirus.	during permissive infection, human cytomegalovirus (hcmv) increases the activities of certain cellular enzymes. in this report, we show that induction of these cellular genes occurs at the level of rna. the abundance of several additional growth-regulated cellular transcripts in hcmv-infected human foreskin fibroblasts (hff) was also compared to that in serum-stimulated hff cells. hcmv infection of hff cells resulted in increased abundance of ornithine decarboxylase, thymidine kinase, heat-shock ...	1988	2842946
detection of leukotriene b4 in cardiac tissue and its role in infarct extension through leucocyte migration.	the main left coronary artery of rats was ligated near its origin under light ether anaesthesia and the infarction observed for 48 h. the ischaemic area was determined after an intravenous injection of pontamine sky blue dye 1 h before induction of cardiac arrest with potassium chloride. the unstained area (true ischaemic area) decreased with time to 27.1% of the left ventricle at 48 h, whereas the intensely stained area between the normal and the true ischaemic areas increased with time, sugges ...	1988	2844405
prenatal diazepam exposure affects beta-adrenergic receptors in brain regions of adult rat offspring.	the postnatal development of [3h]dihydroalprenolol binding to beta-adrenergic receptors has been studied in frontal cortex, cerebellum, striatum, and hypothalamus of the rat after prenatal and perinatal exposure to diazepam. dams were injected subcutaneously with single daily doses of 1 mg of diazepam/kg from day 7 to 20 of gestation or from day 15 of gestation to day 6 after birth. prenatal exposure had no effect on litter size or length of gestation or on the postnatal development of body and ...	1988	2844991
virulence-associated genetic regions comprising 31 kilobases of the 230-kilobase plasmid in shigella flexneri 2a.	by random transposon tn5 insertions, we previously identified six virulence-associated sali fragments, b, d, f, g, h, and p, in the 230-kilobase plasmid pmysh6000 of shigella flexneri 2a. in this study, we analyzed the sites of 134 independent tn5 insertions on four contiguous sali fragments, b, p, h, and d, of pmysh6000 and identified five virulence-associated regions; four were associated with inducing a positive sereny test (ser), invasion into epithelial cells (inv), binding to congo red (pc ...	1988	2836357
molecular basis of sindbis virus neurovirulence in mice.	we examined a variety of strains of sindbis virus for the genetic changes responsible for differences in neurovirulence in mice. sv1a (a low passage of the ar339 strain of sindbis virus), a neuroadapted sindbis virus (nsv), and two laboratory strains of sindbis virus (hrsp and toto1101) were examined. nsv causes severe encephalomyelitis with hind-limb paralysis and high mortality after intracerebral inoculation in weanling mice. in contrast, sv1a causes only mild, nonfatal disease in weanling mi ...	1988	2836615
development of an auxotrophic oral live shigella flexneri vaccine.	an oral live attenuated shigella flexneri vaccine candidate strain was constructed by making it auxotrophic and dependent on aromatic metabolites not available in mammalian tissues. an arod gene of escherichia coli k12 strain nk 5131, inactivated by insertion in it of the tn 10 transposon, was transduced using phage p1 into a virulent s. flexneri serotype y strain (sfl 1) isolated from a patient with bacillary dysentery. one of the transductant strains sfl 114 was found to invade hela cells in v ...	1988	2838986
the structure of the human skin fibroblast collagenase gene.	genomic clones containing the complete gene encoding human fibroblast interstitial collagenase were isolated from a lambda phage human dna library. the gene is comprised from 10 exons and spans 8.2 kilobase pairs. we have mapped the relative positions and determined the dna sequence of all the exon/intron borders of the gene. the organization of the human interstitial collagenase gene is very similar to that of rabbit collagenase and of two other extracellular matrix (ecm) metalloproteases: rat ...	1988	2839503
the structure of the human skin fibroblast collagenase gene.	genomic clones containing the complete gene encoding human fibroblast interstitial collagenase were isolated from a lambda phage human dna library. the gene is comprised from 10 exons and spans 8.2 kilobase pairs. we have mapped the relative positions and determined the dna sequence of all the exon/intron borders of the gene. the organization of the human interstitial collagenase gene is very similar to that of rabbit collagenase and of two other extracellular matrix (ecm) metalloproteases: rat ...	1988	2839503