Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| point-of-care system for detection of mycobacterium tuberculosis and rifampin resistance in sputum samples. | early detection of mycobacterium tuberculosis complex (mtbc) and markers conveying drug resistance can have a beneficial impact on preventive public health actions. we describe here a new molecular point-of-care (poc) system, the genedrive, which is based on simple sample preparation combined with pcr to detect mtbc and simultaneously detect mutation markers in the rpob gene directly from raw sputum sample. hybridization probes were used to detect the presence of the key mutations in codons 516, ... | 2014 | 24478480 |
| development of a novel selective and differential medium for the isolation of listeria monocytogenes. | a new medium (lecithin and levofloxacin [ll] medium) is described for the isolation of listeria monocytogenes from food samples. ll medium includes lecithin from soybeans for the detection of phosphatidylinositol-specific phospholipase c (pi-plc) and phosphatidylcholine-specific phospholipase c (pc-plc) produced by l. monocytogenes. levofloxacin is incorporated to inhibit the growth of microorganisms other than l. monocytogenes, especially bacillus cereus, shown to possess pi-plc and pc-plc acti ... | 2014 | 24271177 |
| plasmid-mediated quinolone resistance genes in enterobacteriaceae from american crows: high prevalence of bacteria with variable qnrb genes. | 2014 | 24247140 | |
| emergence of escherichia coli sequence type 131 isolates producing kpc-2 carbapenemase in china. | twenty-two kpc-2-producing escherichia coli isolates were obtained from three hospitals in hangzhou, china, from 2007 to 2011. one isolate, with ompc porin deficiency, exhibited high-level carbapenem resistance. pulsed-field gel electrophoresis showed that few isolates were indistinguishable or closely related. multilocus sequence typing indicated that sequence type 131 (st131) was the predominant type (9 isolates, 40.9%), followed by st648 (5 isolates), st405 (2 isolates), st38 (2 isolates), an ... | 2014 | 24323475 |
| detection of carbapenemase-producing bacteria by using an ultra-performance liquid chromatography-tandem mass spectrometry method. | the emergence of carbapenemase-producing bacteria poses a new challenge in the management of antibiotic therapies for patients. this report describes a new method using ultra-performance liquid chromatography-tandem mass spectrometry (uplc-ms/ms) for rapid detection of carbapenemase activity in enterobacteria, pseudomonas aeruginosa, and acinetobacter baumannii. in a panel of 78 isolates, including 41 carbapenemase-producing strains, the ulpc-ms/ms assay showed 100% agreement with molecular char ... | 2014 | 24295978 |
| antibiotic treatment of infections due to carbapenem-resistant enterobacteriaceae: systematic evaluation of the available evidence. | we sought to evaluate the effectiveness of the antibiotic treatment administered for infections caused by carbapenemase-producing enterobacteriaceae. the pubmed and scopus databases were systematically searched. articles reporting the clinical outcomes of patients infected with carbapenemase-producing enterobacteriaceae according to the antibiotic treatment administered were eligible. twenty nonrandomized studies comprising 692 patients who received definitive treatment were included. almost all ... | 2014 | 24080646 |
| in vitro phytochemical, antibacterial, and antifungal activities of leaf, stem, and root extracts of adiantum capillus veneris. | adiantum capillus veneris is a medicinally essential plant used for the treatment of diverse infectious diseases. the study of phytochemical and antimicrobial activities of the plant extracts against multidrug-resistant (mdr) bacteria and medically important fungi is of immense significance. extracts from the leaves, stems, and roots of adiantum capillus veneris were extracted with water, methanol, ethanol, ethyl acetate, and hexane and screened for their antimicrobial activity against ten mdr b ... | 2014 | 24592156 |
| use of the delta neutrophil index as a prognostic factor of mortality in patients with spontaneous bacterial peritonitis: implications of a simple and useful marker. | spontaneous bacterial peritonitis (sbp) is a common and life-threatening infection in patients with advanced cirrhosis. the prognostic value of a novel marker, the delta neutrophil index (dni), was investigated relative to mortality in patients with sbp. | 2014 | 24466280 |
| antibiogram for haemodialysis catheter-related bloodstream infections. | background. haemodialysis (hd) catheter-related bloodstream infections (crbsis) are a major complication of long-term catheter use in hd. this study identified the epidemiology of hd crbsis and to aid in the choice of empiric antibiotics therapy given to patients with hd crbsis. methods. patients with hd crbsis were identified. their blood cultures were performed according to standard sterile technique. specimens were sent to the microbiology lab for culture and sensitivity testing. results were ... | 2014 | 24587904 |
| glycerol dehydrogenase plays a dual role in glycerol metabolism and 2,3-butanediol formation in klebsiella pneumoniae. | glycerol dehydrogenase (gdh) is an important polyol dehydrogenase for glycerol metabolism in diverse microorganisms and for value-added utilization of glycerol in the industry. two gdhs from klebsiella pneumoniae, dhad and glda, were expressed in escherichia coli, purified and characterized for substrate specificity and kinetic parameters. both dhad and glda could catalyze the interconversion of (3r)-acetoin/(2r,3r)-2,3-butanediol or (3s)-acetoin/meso-2,3-butanediol, in addition to glycerol oxid ... | 2014 | 24429283 |
| host-defense peptides with therapeutic potential from skin secretions of frogs from the family pipidae. | skin secretions from frogs belonging to the genera xenopus, silurana, hymenochirus, and pseudhymenochirus in the family pipidae are a rich source of host-defense peptides with varying degrees of antimicrobial activities and cytotoxicities to mammalian cells. magainin, peptide glycine-leucine-amide (pgla), caerulein-precursor fragment (cpf), and xenopsin-precursor fragment (xpf) peptides have been isolated from norepinephrine-stimulated skin secretions from several species of xenopus and silurana ... | 2014 | 24434793 |
| piper nigrum leaf and stem assisted green synthesis of silver nanoparticles and evaluation of its antibacterial activity against agricultural plant pathogens. | utilization of biological materials in synthesis of nanoparticles is one of the hottest topics in modern nanoscience and nanotechnology. in the present investigation, the silver nanoparticles were synthesized by using the leaf and stem extract of piper nigrum. the synthesized nanoparticle was characterized by uv-vis spectroscopy, x-ray diffraction (xrd), scanning electron microscope (sem), transmission electron microscope (tem), energy dispersive x-ray analysis (edax), and fourier transform infr ... | 2014 | 24558336 |
| the role of intestinal microbiota in development of irinotecan toxicity and in toxicity reduction through dietary fibres in rats. | cpt-11 is a drug used as chemotherapy for colorectal cancer. cpt-11 causes toxic side-effects in patients. cpt-11 toxicity has been attributed to the activity of intestinal microbiota, however, intestinal microbiota may also have protective effects in cp!-11 chemotherapy. this study aimed to elucidate mechanisms through which microbiota and dietary fibres could modify host health. rats bearing a ward colon carcinoma were treated with a two-cycle cpt-11/5-fluorouracil therapy recapitulating clini ... | 2014 | 24454707 |
| polymicrobial bloodstream infection in neonates: microbiology, clinical characteristics, and risk factors. | polymicrobial bloodstream infections (pbsis) have been associated with complex underlying medical conditions and a high incidence of specific microorganisms in several settings, but the relevant data are scarce in neonates. | 2014 | 24454692 |
| aetiologic agents of fevers of unknown origin among patients in benin city, nigeria. | malaria parasitaemia is an important predictor of bacteremia, concomitant invasive bacterial infections and malaria parasitaemia are strongly associated with death. | 2014 | 24639610 |
| rapid whole-genome sequencing for detection and characterization of microorganisms directly from clinical samples. | whole-genome sequencing (wgs) is becoming available as a routine tool for clinical microbiology. if applied directly on clinical samples, this could further reduce diagnostic times and thereby improve control and treatment. a major bottleneck is the availability of fast and reliable bioinformatic tools. this study was conducted to evaluate the applicability of wgs directly on clinical samples and to develop easy-to-use bioinformatic tools for the analysis of sequencing data. thirty-five random u ... | 2014 | 24172157 |
| first report of bla(imp-8) in raoultella planticola. | two carbapenem-resistant raoultella planticola clinical isolates were isolated from patients with pneumonia and port-a catheter-related bacteremia, respectively, in taiwan. these isolates remained susceptible to fluoroquinolone, aminoglycoside, and colistin. though the two isolates had the same antibiogram, plasmidic carbapenemase blaimp-8, class 1 integron cassette (dfra12-orff-aada2), and qnrb2, they had different pulsed-field gel electrophoresis patterns, plasmid sizes, and outer membrane pro ... | 2014 | 24145534 |
| comparison of an antigen-capture enzyme-linked immunosorbent assay with bacterial culture for detection of salmonella in poultry-hatchery environmental samples. | an antigen-capture, monoclonal-antibody-based enzyme-linked immunoassay (elisa) that detects a broad range of salmonella serovars in various serogroups was developed and compared with standard culture procedures for detection of salmonella in 1055 field samples collected from poultry-hatchery environments. the diagnostic sensitivity of the elisa relative to culture was 99.9% and the diagnostic specificity 99.6%. the extensive culture procedure included nonselective enrichment (nse) as well as pr ... | 2014 | 24396183 |
| monitoring in vitro antibacterial efficacy of 26 indian spices against multidrug resistant urinary tract infecting bacteria. | to screen methanolic extracts of 26 commonly used indian spices against nine species of uropathogenic bacteria (enterococcus faecalis, staphylococcus aureus, acinetobacter baumannii, citrobacter freundii, enterobacter aerogenes, escherichia coli, klebsiella pneumoniae, proteus mirabilis, and pseudomonas aeruginosa), isolated from clinical samples of a tertiary care hospital for antibacterial activity. | 2014 | 28664089 |
| detection of amp c genes encoding for beta-lactamases in escherichia coli and klebsiella pneumoniae. | amp c beta-lactamase are ambler class c enzymes that confer resistance to extended spectrum cephalosporins and are not inhibited by beta-lactamase inhibitors. their detection is crucial, since the phenotypic tests are not standardised leading to ambiguity in interpretation of results. this study was done to detect the types of amp c prevalent in escherichia coli and klebsiella pneumoniae by multiplex polymerase chain reaction (pcr). | 2014 | 22885194 |
| unraveling the dha cluster in citrobacter werkmanii: comparative genomic analysis of bacterial 1,3-propanediol biosynthesis clusters. | in natural 1,3-propanediol (pdo) producing microorganisms such as klebsiella pneumoniae, citrobacter freundii and clostridium sp., the genes coding for pdo producing enzymes are grouped in a dha cluster. this article describes the dha cluster of a novel candidate for pdo production, citrobacter werkmanii dsm17579 and compares the cluster to the currently known pdo clusters of enterobacteriaceae and clostridiaceae. moreover, we attribute a putative function to two previously unannotated orfs, orf ... | 2014 | 23996279 |
| biological characteristics and oxidation mechanism of a new manganese-oxidizing bacteria fm-2. | a new manganese-oxidizing strain fm-2 was screened out from biological activated carbon (bac) filter column and was identified as citrobacter freundii. the results of the systematic study on this species are as follows: at 27°c, the optimum ph for citrobacter sp. fm-2 to remove manganese was 7.0-8.0.the best removal rate of manganese under 27°c, ph 7.0 by fm-2 was reached at 4 d, being 76.2%; compared with adsorption, biological oxidation played a dominant role in this removing process. almost 7 ... | 2014 | 24211955 |
| extended spectrum β-lactamase-producing enterobacteriaceae infection in heart and lung transplant recipients and in mechanical circulatory support recipients. | extended spectrum β-lactamase (esbl)-producing gram-negative bacilli are increasingly reported in patients with a variety of risk factors including prior cephalosporin and antibiotic usage, prolonged hospitalizations, existence of comorbid conditions, and critical illness. | 2014 | 24162251 |
| characterization of a bacteriocin produced by lactococcus lactis subsp. lactis crl 1584 isolated from a lithobates catesbeianus hatchery. | lactococcus lactis crl 1584 isolated from a lithobates catesbeianus hatchery inhibits the growth of citrobacter freundii (a bullfrog pathogen) and listeria monocytogenes by a synergistic effect between lactic acid, hydrogen peroxide and a bacteriocin-like molecule. the chemical characterization of the bacteriocin in cell-free supernatants indicates that it has a proteinaceous nature. hexadecane and ethyl acetate did not modify the bacteriocin activity, while 10 and 20 % (v/v) chloroform decrease ... | 2014 | 24150985 |
| diversity of enterobacteria including β-lactamase producing isolates associated with the spanish slug (arion vulgaris). | the aim of this study was to determine diversity in members of the family enterobacteriaceae including the beta-lactamase producing isolates associated with the spanish slug (arion vulgaris). 81 isolates, each representing a distinct colonial morphotype, were obtained from fecal samples of spanish slugs and analyzed. genetically heterogeneous or similar groups were assessed among the isolates by enterobacterial repetitive intergenic consensus (eric)-pcr and further characterized by 16s rdna sequ ... | 2014 | 24534696 |
| crystal structure of the external aldimine of citrobacter freundii methionine γ-lyase with glycine provides insight in mechanisms of two stages of physiological reaction and isotope exchange of α- and β-protons of competitive inhibitors. | the three-dimensional structure of the external aldimine of citrobacter freundii methionine γ-lyase with competitive inhibitor glycine has been determined at 2.45 å resolution. it revealed subtle conformational changes providing effective binding of the inhibitor and facilitating labilization of cα-protons of the external aldimine. the structure shows that 1, 3-prototropic shift of cα-proton to c4'-atom of the cofactor may proceed with participation of active site lys210 residue whose location i ... | 2014 | 24463191 |
| impact of cold plasma on citrobacter freundii in apple juice: inactivation kinetics and mechanisms. | various studies have shown that cold plasma is capable of inactivating microorganisms located on a variety of food surfaces, food packaging materials and process equipment under atmospheric pressure conditions; however, less attention has been paid to the impact of cold plasma on microorganisms in liquid foodstuffs. the present study investigates cold plasma's ability to inactivate citrobacter freundii in apple juice. optical emission spectroscopy (oes) and temperature measurements were performe ... | 2014 | 24462703 |
| characterization of kpc-2-producing escherichia coli, citrobacter freundii, enterobacter cloacae, enterobacter aerogenes, and klebsiella oxytoca isolates from a chinese hospital. | twelve nonduplicated kpc-2-producing enterobacterial isolates, including three escherichia coli, two citrobacter freundii, two enterobacter cloacae, four enterobacter aerogenes, and one klebsiella oxytoca, were collected from various clinical samples within 18 months (march 2011 to september 2012). two of the 12 patients died from infections caused by kpc-2-producing pathogens, while the rest of the patients with kpc-2-producing pathogens improved or were cured. the majority of the clinical isol ... | 2014 | 24433026 |
| in vitro temocillin efficacy against extended spectrum β-lactamase producing multidrug resistant gram negative bacterial isolates from nepal. | temocillin is relatively more stable against most β-lactamases and requires re-evaluation to include it in common clinical practice as a therapeutic alternative. at the national reference laboratory of nepal, we evaluated multidrug resistance (mdr) and extended spectrum β-lactamase (esbl) phenotypes among 292 gram-negative clinical bacterial isolates of 18 different genera during 2009/2010 by kirby-bauer disc diffusion method following clsi guidelines. esbl screen positive isolates were tested f ... | 2014 | 26930733 |
| measurement of biochemical oxygen demand from different wastewater samples using a mediator-less microbial fuel cell biosensor. | microbial fuel cells (mfcs) have attracted considerable attention as potential biosensors. a mfc biosensor for rapid measurement of biochemical oxygen demand (bod) has been recently studied. however, a standardized bacterial mixture inoculated in the mfc biosensor for bod measurement is unavailable. thus, the commercial application of a mfc biosensor is limited. in this study, a mediator-less mfc biosensor inoculated with known mixed cultures to quickly determine bod concentration was tested. op ... | 2014 | 25145173 |
| observations on citrobacter species from a tertiary care health center with special reference to multi-drug resistance and presence of ctx-m gene. | citrobacter is an important nosocomial pathogen and its multi-drug resistant (mdr) isolates are increasingly being reported across the globe. they are known to produce extended spectrum beta lactamase (esbl) and harbor ctx-m gene. | 2014 | 25118739 |
| characterization of a virulent bacteriophage lk1 specific for citrobacter freundii isolated from sewage water. | citrobacter freundii is a worldwide emerging nosocomial pathogen with escalating incidence of multidrug resistance. citrobacter freundii exists in natural environment, especially in health care settings and is difficult to eradicate. phage therapy is considered as an alternative way of controlling bacterial infections and contaminations. in this study, we have described isolation and characterization of a virulent bacteriophage lk1 capable of specifically infecting citrobacter freundii. a virule ... | 2014 | 23686910 |
| severe bacterial infection in patients with heterotaxy syndrome. | to determine the incidence of sepsis in patients with heterotaxy syndrome. | 2014 | 24112867 |
| elevated 3-hydroxypropionaldehyde production from glycerol using a citrobacter freundii mutant. | a mutant strain of citrobacter freundii capable of elevated 3-hydroxypropionaldehyde production from glycerol was isolated using chemical mutagenesis and a screening protocol. the protocol involved screening mutagenized bacterial cells on solid minimal medium containing 5 % (v/v) glycerol. colonies were picked onto duplicate solid minimal medium plates and one plate was stained with 1 % (w/v) phloroglucinol. those colonies staining red were further screened and a mutant, hpao-1, was identified. ... | 2014 | 24062134 |
| host-specific induction of escherichia coli fitness genes during human urinary tract infection. | uropathogenic escherichia coli (upec) is the predominant etiological agent of uncomplicated urinary tract infection (uti), manifested by inflammation of the urinary bladder, in humans and is a major global public health concern. molecular pathogenesis of upec has been primarily examined using murine models of uti. translational research to develop novel therapeutics against this major pathogen, which is becoming increasingly antibiotic resistant, requires a thorough understanding of mechanisms i ... | 2014 | 25489107 |
| real-time comparative evaluation of biomerieux vitek ms versus bruker microflex ms, two matrix-assisted laser desorption-ionization time-of-flight mass spectrometry systems, for identification of clinically significant bacteria. | matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (maldi-tof ms) recently became available for the identification of bacteria in routine diagnostic laboratories. it is rapid and cost-effective and likely to replace phenotypic identification. this study was undertaken to compare two maldi-tof ms-based, bruker microflex ms (bms) and vitek ms (vms) systems, for identification (id) of clinically significant bacterial isolates. clinically relevant broad diversity of bacteri ... | 2014 | 25433488 |
| high-risk enteric pathogens associated with hiv infection and hiv exposure in kenyan children with acute diarrhoea. | hiv infection is an established risk for diarrhoeal severity, less is known about specific enteric pathogens associated with hiv status. we determined associations of selected enteric pathogens with hiv infection and hiv exposure among kenyan children. | 2014 | 25028987 |
| mutation of a salmonella serogroup-c1-specific gene abrogates o7-antigen biosynthesis and triggers nacl-dependent motility deficiency. | several molecular detection marker genes specific for a number of individual salmonella serogroups have been recently identified in our lab by comparative genomics for the genotyping of diverse serogroups. to further understand the correlation between serotype and genotype, the function of a salmonella serogroup-c1-specific gene (sc_2092) was analyzed in this study. it was indicated from the topological prediction using the deduced amino acid sequence of sc_2092 that this putative protein was hi ... | 2014 | 25211341 |
| pentaplex pcr assay for detection of hemorrhagic bacteria from stool samples. | diarrheal diseases cause illness and death among children younger than 10 years in developing countries. conventional testing for the detection of hemorrhagic bacteria takes 2 to 5 days to yield complete information on the organism and its antibiotic sensitivity pattern. hence, in the present study, we developed a molecular-based diagnostic assay that identifies common hemorrhagic bacteria in stool samples. a set of specific primers were designed for the detection of salmonella spp., shigella sp ... | 2014 | 24958797 |
| detection of tetracycline resistance genes in bacteria isolated from fish farms using polymerase chain reaction. | five common tetracycline resistance genes tet(a), tet(b), tet(m), tet(o) and tet(s) were studied by polymerase chain reaction in 100 bacteria isolated from iranian fish farms. in the antibiogram test most of the bacteria were either intermediately or completely resistant to tetracycline. nine isolates out of 46 aeromonas spp. contained either tet(a/m/s) resistant genes as follows: tet(a) in a. veronii/sobria (n = 1), a. media (n = 2), a. aquariorum (n = 1), and a. veronii (n = 3); tet(m) in one ... | 2014 | 25610578 |
| cytotoxic and pathogenic properties of klebsiella oxytoca isolated from laboratory animals. | klebsiella oxytoca is an opportunistic pathogen implicated in various clinical diseases in animals and humans. studies suggest that in humans k. oxytoca exerts its pathogenicity in part through a cytotoxin. however, cytotoxin production in animal isolates of k. oxytoca and its pathogenic properties have not been characterized. furthermore, neither the identity of the toxin nor a complete repertoire of genes involved in k. oxytoca pathogenesis have been fully elucidated. here, we showed that seve ... | 2014 | 25057966 |
| evaluation of three rapid diagnostic methods for direct identification of microorganisms in positive blood cultures. | the identification of organisms from positive blood cultures generally takes several days. however, recently developed rapid diagnostic methods offer the potential for organism identification within only a few hours of blood culture positivity. in this study, we evaluated the performance of three commercial methods to rapidly identify organisms directly from positive blood cultures: quickfish (advandx, wolburn, ma), verigene gram-positive blood culture (bc-gp; nanosphere, northbrook, il), and ma ... | 2014 | 24808235 |
| the focal complex of epithelial cells provides a signalling platform for interleukin-8 induction in response to bacterial pathogens. | bacterial pathogens can induce an inflammatory response from epithelial tissues due to secretion of the pro-inflammatory chemokine interleukin-8 (il-8). many bacterial pathogens manipulate components of the focal complex (fc) to induce signalling events in host cells. we examined the interaction of several bacterial pathogens with host cells, including campylobacter jejuni, to determine if the fc is required for induction of chemokine signalling in response to bacterial pathogens. our data indic ... | 2014 | 24779413 |
| the arable ecosystem as battleground for emergence of new human pathogens. | disease incidences related to escherichia coli and salmonella enterica infections by consumption of (fresh) vegetables, sprouts, and occasionally fruits made clear that these pathogens are not only transmitted to humans via the "classical" routes of meat, eggs, and dairy products, but also can be transmitted to humans via plants or products derived from plants. nowadays, it is of major concern that these human pathogens, especially the ones belonging to the taxonomical family of enterobacteriace ... | 2014 | 24688484 |
| the role of autophagy in the intracellular survival of campylobacter concisus. | campylobacter concisus is an emerging pathogen that has been associated with gastrointestinal diseases. given the importance of autophagy for the elimination of intracellular bacteria and the subversion of this process by pathogenic bacteria, we investigated the role of autophagy in c. concisus intracellular survival. gentamicin protection assays were employed to assess intracellular levels of c. concisus within caco-2 cells, following autophagy induction and inhibition. to assess the interactio ... | 2014 | 24918042 |
| a loop-mediated isothermal amplification (lamp) assay for strongyloides stercoralis in stool that uses a visual detection method with syto-82 fluorescent dye. | an assay to detect strongyloides stercoralis in stool specimens was developed using the loop-mediated isothermal amplification (lamp) method. primers were based on the 28s ribosomal subunit gene. the reaction conditions were optimized and syto-82 fluorescent dye was used to allow real-time and visual detection of the product. the product identity was confirmed with restriction enzyme digestion, cloning, and sequence analysis. the assay was specific when tested against dna from bacteria, fungi an ... | 2014 | 24323513 |
| mucosa-associated bacterial microbiome of the gastrointestinal tract of weaned pigs and dynamics linked to dietary calcium-phosphorus. | dietary composition largely influences pig's gastrointestinal microbiota and represents a useful prophylactic tool against enteric disturbances in young pigs. despite the importance for host-microbe interactions and bacterial colonization, dietary responses of the mucosa-associated bacterial communities are less well investigated. in the present study, we characterized the mucosa-associated bacterial communities at the pars non-glandularis of the stomach, ileum and colon, and identified shifts i ... | 2014 | 24466298 |
| a low complexity rapid molecular method for detection of clostridium difficile in stool. | here we describe a method for the detection of clostridium difficile from stool using a novel low-complexity and rapid extraction process called heat elution (he). the he method is two-step and takes just 10 minutes, no specialist instruments are required and there is minimal hands-on time. a test method using he was developed in conjunction with loop-mediated isothermal amplification (lamp) combined with the real-time bioluminescent reporter system known as bart targeting the toxin b gene (tcdb ... | 2014 | 24416173 |
| diverse bacterial microcompartment organelles. | bacterial microcompartments (mcps) are sophisticated protein-based organelles used to optimize metabolic pathways. they consist of metabolic enzymes encapsulated within a protein shell, which creates an ideal environment for catalysis and facilitates the channeling of toxic/volatile intermediates to downstream enzymes. the metabolic processes that require mcps are diverse and widely distributed and play important roles in global carbon fixation and bacterial pathogenesis. the protein shells of m ... | 2014 | 25184561 |
| the importance of the viable but non-culturable state in human bacterial pathogens. | many bacterial species have been found to exist in a viable but non-culturable (vbnc) state since its discovery in 1982. vbnc cells are characterized by a loss of culturability on routine agar, which impairs their detection by conventional plate count techniques. this leads to an underestimation of total viable cells in environmental or clinical samples, and thus poses a risk to public health. in this review, we present recent findings on the vbnc state of human bacterial pathogens. the characte ... | 2014 | 24917854 |
| evaluation of a loop-mediated isothermal amplification suite for the rapid, reliable, and robust detection of shiga toxin-producing escherichia coli in produce. | shiga toxin-producing escherichia coli (stec) strains are a leading cause of produce-associated outbreaks in the united states. rapid, reliable, and robust detection methods are needed to better ensure produce safety. we recently developed a loop-mediated isothermal amplification (lamp) suite for stec detection. in this study, the stec lamp suite was comprehensively evaluated against real-time quantitative pcr (qpcr) using a large panel of bacterial strains (n = 156) and various produce items (s ... | 2014 | 24509927 |
| culturable aerobic and facultative anaerobic intestinal bacterial flora of black cobra (naja naja karachiensis) in southern pakistan. | using morphological analysis and biochemical testing, here for the first time, we determined the culturable gut bacterial flora (aerobes and facultative anaerobes) in the venomous black cobra (naja naja karachiensis) from south asia. the findings revealed that these snakes inhabit potentially pathogenic bacteria including serratia marcescens, pseudomonas aeruginosa, shewanella putrefaciens, aeromonas hydrophila, salmonella sp., moraxella sp., bacillus sp., ochrobactrum anthropi, and providencia ... | 2014 | 25002979 |
| efficient routes toward the synthesis of the d-rhamno-trisaccharide related to the a-band polysaccharide of pseudomonas aeruginosa. | the present work describes efficient avenues for the synthesis of the trisaccharide repeating unit [α-d-rhap-(1→3)-α-d-rhap-(1→3)-α-d-rhap] associated with the a-band polysaccharide of pseudomonas aeruginosa. one of the key steps involved 6-o-deoxygenation of either partially or fully acylated 4,6-o-benzylidene-1-thiomannopyranoside by radical-mediated redox rearrangement in high yields and regioselectivity. the d-rhamno-thioglycosides so obtained allowed efficient access to the trisaccharide ta ... | 2014 | 25161705 |
| impurities of crude glycerol and their effect on metabolite production. | glycerol is a valuable raw material for the production of industrially useful metabolites. among many promising applications for the use of glycerol is its bioconversion to high value-added compounds, such as 1,3-propanediol (1,3-pd), succinate, ethanol, propionate, and hydrogen, through microbial fermentation. another method of waste material utilization is the application of crude glycerol in blends with other wastes (e.g., tomato waste hydrolysate). however, crude glycerol, a by-product of bi ... | 2014 | 25100926 |
| impurities of crude glycerol and their effect on metabolite production. | glycerol is a valuable raw material for the production of industrially useful metabolites. among many promising applications for the use of glycerol is its bioconversion to high value-added compounds, such as 1,3-propanediol (1,3-pd), succinate, ethanol, propionate, and hydrogen, through microbial fermentation. another method of waste material utilization is the application of crude glycerol in blends with other wastes (e.g., tomato waste hydrolysate). however, crude glycerol, a by-product of bi ... | 2014 | 25100926 |
| fast and sensitive detection of enteropathogenic yersinia by immunoassays. | yersinia enterocolitica and yersinia pseudotuberculosis, the two yersinia species that are enteropathogenic for humans, are distributed worldwide and frequently cause diarrhea in inhabitants of temperate and cold countries. y. enterocolitica is a major cause of foodborne disease resulting from consumption of contaminated pork meat and is further associated with substantial economic cost. however, investigation of enteropathogenic yersinia species is infrequently performed routinely in clinical l ... | 2014 | 25355759 |
| fast and sensitive detection of enteropathogenic yersinia by immunoassays. | yersinia enterocolitica and yersinia pseudotuberculosis, the two yersinia species that are enteropathogenic for humans, are distributed worldwide and frequently cause diarrhea in inhabitants of temperate and cold countries. y. enterocolitica is a major cause of foodborne disease resulting from consumption of contaminated pork meat and is further associated with substantial economic cost. however, investigation of enteropathogenic yersinia species is infrequently performed routinely in clinical l ... | 2014 | 25355759 |
| in silico detection and typing of plasmids using plasmidfinder and plasmid multilocus sequence typing. | in the work presented here, we designed and developed two easy-to-use web tools for in silico detection and characterization of whole-genome sequence (wgs) and whole-plasmid sequence data from members of the family enterobacteriaceae. these tools will facilitate bacterial typing based on draft genomes of multidrug-resistant enterobacteriaceae species by the rapid detection of known plasmid types. replicon sequences from 559 fully sequenced plasmids associated with the family enterobacteriaceae i ... | 2014 | 24777092 |
| temporal changes in the bacterial community of animal feces and their correlation with stable fly oviposition, larval development, and adult fitness. | stable flies are blood-feeding insects with a great negative impact on animals world wide. larvae develop primarily in animal manure and bacteria are essential for larval development; however, the principle of this dependence is not understood. we hypothesized that as the microbial community of animal manure changes over time, it plays an important role in stable fly fitness. two-choice bioassays were conducted using 2 week old horse manure (control) and aging horse manure (fresh to 5 week old) ... | 2014 | 25426108 |
| novel selective medium for isolation of staphylococcus lugdunensis from wound specimens. | we compared a novel selective staphylococcus lugdunensis (ssl) medium with routine media (blood and chocolate agars) for the detection of s. lugdunensis in 990 clinical specimens (from tissue, pus, or wound swabs). significantly more s. lugdunensis isolates were detected on ssl medium (34/990) than on routine medium (7/990) (p = 0.001, mcnemar's test). | 2014 | 24759715 |
| the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in identifying clinically relevant bacteria: a comparison with automated microbiology system. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has been developed as a new-type soft ionization mass spectrometry in the recent year. increasing number of clinical microbiological laboratories consider it as an innovate approach for bacterial identification. | 2014 | 24822117 |
| bacteriology and drug susceptibility analysis of pus from patients with severe intra-abdominal infection induced by abdominal trauma. | the aim of the present study was to retrospectively analyze the bacteriology and drug susceptibility of pus flora from abdominal trauma patients with severe intra-abdominal infection (siai). a total of 41 patients with siai induced by abdominal trauma were enrolled in the study, from which 123 abdominal pus samples were obtained. the results from laboratory microbiology and drug sensitivity were subjected to susceptibility analysis using whonet software. a total of 297 strains were isolated in w ... | 2014 | 24940451 |
| the construction and evaluation of reference spectra for the identification of human pathogenic microorganisms by maldi-tof ms. | matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is an emerging technique for the rapid and high-throughput identification of microorganisms. there remains a dearth of studies in which a large number of pathogenic microorganisms from a particular country or region are utilized for systematic analyses. in this study, peptide mass reference spectra (pmrs) were constructed and evaluated from numerous human pathogens (a total of 1019 strains from 94 species ... | 2014 | 25181391 |
| a quantitative prioritisation of human and domestic animal pathogens in europe. | disease or pathogen risk prioritisations aid understanding of infectious agent impact within surveillance or mitigation and biosecurity work, but take significant development. previous work has shown the h-(hirsch-)index as an alternative proxy. we present a weighted risk analysis describing infectious pathogen impact for human health (human pathogens) and well-being (domestic animal pathogens) using an objective, evidence-based, repeatable approach; the h-index. this study established the highe ... | 2014 | 25136810 |
| strand invasion based amplification (siba®): a novel isothermal dna amplification technology demonstrating high specificity and sensitivity for a single molecule of target analyte. | isothermal nucleic acid amplification technologies offer significant advantages over polymerase chain reaction (pcr) in that they do not require thermal cycling or sophisticated laboratory equipment. however, non-target-dependent amplification has limited the sensitivity of isothermal technologies and complex probes are usually required to distinguish between non-specific and target-dependent amplification. here, we report a novel isothermal nucleic acid amplification technology, strand invasion ... | 2014 | 25419812 |
| pre-steady-state kinetic and structural analysis of interaction of methionine γ-lyase from citrobacter freundii with inhibitors. | methionine γ-lyase (mgl) catalyzes the γ-elimination of l-methionine and its derivatives as well as the β-elimination of l-cysteine and its analogs. these reactions yield α-keto acids and thiols. the mechanism of chemical conversion of amino acids includes numerous reaction intermediates. the detailed analysis of mgl interaction with glycine, l-alanine, l-norvaline, and l-cycloserine was performed by pre-steady-state stopped-flow kinetics. the structure of side chains of the amino acids is impor ... | 2014 | 25398880 |
| pre-steady-state kinetic and structural analysis of interaction of methionine γ-lyase from citrobacter freundii with inhibitors. | methionine γ-lyase (mgl) catalyzes the γ-elimination of l-methionine and its derivatives as well as the β-elimination of l-cysteine and its analogs. these reactions yield α-keto acids and thiols. the mechanism of chemical conversion of amino acids includes numerous reaction intermediates. the detailed analysis of mgl interaction with glycine, l-alanine, l-norvaline, and l-cycloserine was performed by pre-steady-state stopped-flow kinetics. the structure of side chains of the amino acids is impor ... | 2014 | 25398880 |
| l-methionase: a therapeutic enzyme to treat malignancies. | cancer is an increasing cause of mortality and morbidity throughout the world. l-methionase has potential application against many types of cancers. l-methionase is an intracellular enzyme in bacterial species, an extracellular enzyme in fungi, and absent in mammals. l-methionase producing bacterial strain(s) can be isolated by 5,5'-dithio-bis-(2-nitrobenzoic acid) as a screening dye. l-methionine plays an important role in tumour cells. these cells become methionine dependent and eventually fol ... | 2014 | 25250324 |
| ingestibility, digestibility, and engineered biological control potential of flavobacterium hibernum, isolated from larval mosquito habitats. | flavobacterium hibernum, isolated from larval habitats of the eastern tree hole mosquito, a. triseriatus, remained suspended in the larval feeding zone much longer (8 days) than other bacteria. autofluorescent protein markers were developed for the labeling of f. hibernum with a strong flavobacterial expression system. green fluorescent protein (gfp)-tagged f. hibernum cells were quickly consumed by larval mosquitoes at an ingestion rate of 9.5 × 10(4)/larva/h. the ingested f. hibernum cells wer ... | 2014 | 24296502 |
| neutrophils in host defense: new insights from zebrafish. | neutrophils are highly motile phagocytic cells that play a critical role in the immune response to infection. zebrafish (danio rerio) are increasingly used to study neutrophil function and host-pathogen interactions. the generation of transgenic zebrafish lines with fluorescently labeled leukocytes has made it possible to visualize the neutrophil response to infection in real time by use of optically transparent zebrafish larvae. in addition, the genetic tractability of zebrafish has allowed for ... | 2015 | 25717145 |
| development of a new real-time pcr system for simultaneous detection of bacteria and fungi in pathological samples. | a novel system for simultaneous detection of pathogenic bacteria and fungi in pathological samples was developed using a real-time polymerase chain reaction (pcr) system. this system, designated the "multi-microbial real-time pcr", has the potential to simultaneously detect 68 bacterial and 9 fungal species in a 96-well plate format. all probe-primer sets were designed to produce amplicons smaller than 210 bp using formalin-fixed paraffin-embedded samples as input. the specificity and sensitivit ... | 2015 | 26823918 |
| dna microarray characterization of pathogens associated with sexually transmitted diseases. | this study established a multiplex pcr-based microarray to detect simultaneously a diverse panel of 17 sexually transmitted diseases (stds)-associated pathogens including neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma genitalium, mycoplasma hominis, ureaplasma, herpes simplex virus (hsv) types 1 and 2, and human papillomavirus (hpv) types 6, 11, 16, 18, 31, 33, 35, 39, 54 and 58. the target genes are 16s rrna gene for n. gonorrhoeae, m. genitalium, m. hominism, and ureaplasma, the majo ... | 2015 | 26208181 |
| mechanisms of drug resistance: quinolone resistance. | quinolone antimicrobials are synthetic and widely used in clinical medicine. resistance emerged with clinical use and became common in some bacterial pathogens. mechanisms of resistance include two categories of mutation and acquisition of resistance-conferring genes. resistance mutations in one or both of the two drug target enzymes, dna gyrase and dna topoisomerase iv, are commonly in a localized domain of the gyra and pare subunits of the respective enzymes and reduce drug binding to the enzy ... | 2015 | 26190223 |
| epidemiology of neonatal sepsis and implicated pathogens: a study from egypt. | prospective analytic study was conducted in nicus of three egyptian neonatal network (egnn) participants in mansoura hospitals in egypt over a period of 18 months from march 2011 to august 2012. by using egnn 28-day discharge form, all demographic, clinical, and laboratory data were recorded and studied. during the study period, 357 neonates were diagnosed as suspected sepsis with an incidence of 45.9% (357/778) among the admitted neonates at the three neonatal intensive care units. 344 neonates ... | 2015 | 26146621 |
| the challenge of efflux-mediated antibiotic resistance in gram-negative bacteria. | the global emergence of multidrug-resistant gram-negative bacteria is a growing threat to antibiotic therapy. the chromosomally encoded drug efflux mechanisms that are ubiquitous in these bacteria greatly contribute to antibiotic resistance and present a major challenge for antibiotic development. multidrug pumps, particularly those represented by the clinically relevant acrab-tolc and mex pumps of the resistance-nodulation-division (rnd) superfamily, not only mediate intrinsic and acquired mult ... | 2015 | 25788514 |
| performances and reliability of bruker microflex lt and vitek ms maldi-tof mass spectrometry systems for the identification of clinical microorganisms. | in clinical microbiology laboratories, routine microbial identification is mostly performed using culture based methodologies requiring 24 to 72 hours from culturing to identification. matrix assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) technology has been established as a cost effective, reliable, and faster alternative identification platform. in this study, we evaluated the reliability of the two available maldi-tof ms systems for their routine clinical ... | 2015 | 26793718 |
| high voltage electrochemiluminescence (ecl) as a new method for detection of pah during screening for pah-degrading microbial consortia. | the search for new bacterial consortia capable of removing pah from the environment is associated with the need to employ novel, simple, and economically efficient detection methods. a fluorimetric method (fl) as well as high voltage electrochemiluminescence (ecl) on a modified surface of an aluminum electrode were used in order to determine the changes in the concentrations of pah in the studied aqueous solutions. the ecl signal (the spectrum and emission intensity for a given wavelength) was d ... | 2015 | 26213425 |
| bacterial cellulose biosynthesis: diversity of operons, subunits, products, and functions. | recent studies of bacterial cellulose biosynthesis, including structural characterization of a functional cellulose synthase complex, provided the first mechanistic insight into this fascinating process. in most studied bacteria, just two subunits, bcsa and bcsb, are necessary and sufficient for the formation of the polysaccharide chain in vitro. other subunits - which differ among various taxa - affect the enzymatic activity and product yield in vivo by modulating (i) the expression of the bios ... | 2015 | 26077867 |
| evaluation of presurgical skin preparation agents in african clawed frogs (xenopus laevis). | despite the routine collection of oocytes from african clawed frogs (xenopus laevis) for use in research, few studies have evaluated methods for preparing their skin for surgery. we evaluated 3 skin preparatory agents by examining their antibacterial efficacy and the gross and microscopic appearance of xenopus skin after exposure. frogs (n = 14) were sedated and treated (contact time, 10 min) with 0.9% sterile nacl on one-half of the ventrum and with 0.5% povidone-iodine or 0.75% chlorhexidine o ... | 2015 | 26632790 |
| the extended regulatory networks of sxt/r391 integrative and conjugative elements and inca/c conjugative plasmids. | nowadays, healthcare systems are challenged by a major worldwide drug resistance crisis caused by the massive and rapid dissemination of antibiotic resistance genes and associated emergence of multidrug resistant pathogenic bacteria, in both clinical and environmental settings. conjugation is the main driving force of gene transfer among microorganisms. this mechanism of horizontal gene transfer mediates the translocation of large dna fragments between two bacterial cells in direct contact. inte ... | 2015 | 26347724 |
| the relationship between extensively drug-resistant tuberculosis and multidrug-resistant gram-negative bacilli. | the relationship between extensively drug-resistant tuberculosis (xdr-tb) and multidrug-resistant gram-negative bacilli (mdr-gnb) is unclear. identification of the relationship between xdr-tb and mdr-gnb would have important implications for patient care. | 2015 | 26230499 |
| advantages and risks of husbandry and housing changes to improve animal wellbeing in a breeding colony of common marmosets (callithrix jacchus). | between 1975 and 2014, housing conditions for laboratory-housed marmosets changed dramatically after the introduction of new guidelines designed to improve their care and wellbeing. according to these guidelines, our facility provided marmosets with outside enclosures, switched to deep litter as bedding material, and discontinued the use of disinfectant agents in animal enclosures. however, both deep litter and access to outside enclosures hypothetically increase the risk of potential exposure t ... | 2015 | 26045452 |
| mesaconase/fumarase fumd in escherichia coli o157:h7 and promiscuity of escherichia coli class i fumarases fuma and fumb. | mesaconase catalyzes the hydration of mesaconate (methylfumarate) to (s)-citramalate. the enzyme participates in the methylaspartate pathway of glutamate fermentation as well as in the metabolism of various c5-dicarboxylic acids such as mesaconate or l-threo-β-methylmalate. we have recently shown that burkholderia xenovorans uses a promiscuous class i fumarase to catalyze this reaction in the course of mesaconate utilization. here we show that classical escherichia coli class i fumarases a and b ... | 2015 | 26658641 |
| oviposition responses of aedes mosquitoes to bacterial isolates from attractive bamboo infusions. | the mosquitoes aedes aegypti and aedes albopictus are vectors of pathogenic viruses that cause major human illnesses including dengue, yellow fever and chikungunya. both mosquito species are expanding their geographic distributions and now occur worldwide in temperate and tropical climates. collection of eggs in oviposition traps (ovitraps) is commonly used for monitoring and surveillance of container-inhabiting aedes populations by public health agencies charged with managing mosquito-transmitt ... | 2015 | 26399712 |
| use of next generation sequencing data to develop a qpcr method for specific detection of eu-unauthorized genetically modified bacillus subtilis overproducing riboflavin. | recently, the presence of an unauthorized genetically modified (gm) bacillus subtilis bacterium overproducing vitamin b2 in a feed additive was notified by the rapid alert system for food and feed (rasff). this has demonstrated that a contamination by a gm micro-organism (gmm) may occur in feed additives and has confronted for the first time,the enforcement laboratories with this type of rasff. as no sequence information of this gmm nor any specific detection or identification method was availab ... | 2015 | 26559764 |
| mechanisms of resistance to aminoglycoside antibiotics: overview and perspectives. | aminoglycoside (ag) antibiotics are used to treat many gram-negative and some gram-positive infections and, importantly, multidrug-resistant tuberculosis. among various bacterial species, resistance to ags arises through a variety of intrinsic and acquired mechanisms. the bacterial cell wall serves as a natural barrier for small molecules such as ags and may be further fortified via acquired mutations. efflux pumps work to expel ags from bacterial cells, and modifications here too may cause furt ... | 2015 | 26877861 |
| mechanisms of resistance to aminoglycoside antibiotics: overview and perspectives. | aminoglycoside (ag) antibiotics are used to treat many gram-negative and some gram-positive infections and, importantly, multidrug-resistant tuberculosis. among various bacterial species, resistance to ags arises through a variety of intrinsic and acquired mechanisms. the bacterial cell wall serves as a natural barrier for small molecules such as ags and may be further fortified via acquired mutations. efflux pumps work to expel ags from bacterial cells, and modifications here too may cause furt ... | 2015 | 26877861 |
| ecology of anti-biofilm agents ii: bacteriophage exploitation and biocontrol of biofilm bacteria. | bacteriophages are the viruses of bacteria. in the guise of phage therapy they have been used for decades to successfully treat what are probable biofilm-containing chronic bacterial infections. more recently, phage treatment or biocontrol of biofilm bacteria has been brought back to the laboratory for more rigorous assessment as well as towards the use of phages to combat environmental biofilms, ones other than those directly associated with bacterial infections. considered in a companion artic ... | 2015 | 26371011 |
| aptamers against pathogenic microorganisms. | an important current issue of modern molecular medicine and biotechnology is the search for new approaches to early diagnostic assays and adequate therapy of infectious diseases. one of the promising solutions to this problem might be a development of nucleic acid aptamers capable of interacting specifically with bacteria, protozoa, and viruses. such aptamers can be used for the specific recognition of infectious agents as well as for blocking of their functions. the present review summarizes va ... | 2015 | 26258445 |
| aptamers against pathogenic microorganisms. | an important current issue of modern molecular medicine and biotechnology is the search for new approaches to early diagnostic assays and adequate therapy of infectious diseases. one of the promising solutions to this problem might be a development of nucleic acid aptamers capable of interacting specifically with bacteria, protozoa, and viruses. such aptamers can be used for the specific recognition of infectious agents as well as for blocking of their functions. the present review summarizes va ... | 2015 | 26258445 |
| vaccines against enteric infections for the developing world. | since the first licensure of the sabin oral polio vaccine more than 50 years ago, only eight enteric vaccines have been licensed for four disease indications, and all are given orally. while mucosal vaccines offer programmatically attractive tools for facilitating vaccine deployment, their development remains hampered by several factors: -limited knowledge regarding the properties of the gut immune system during early life; -lack of mucosal adjuvants, limiting mucosal vaccine development to live ... | 2015 | 25964464 |
| internalization of pseudomonas aeruginosa strain pao1 into epithelial cells is promoted by interaction of a t6ss effector with the microtubule network. | invasion of nonphagocytic cells through rearrangement of the actin cytoskeleton is a common immune evasion mechanism used by most intracellular bacteria. however, some pathogens modulate host microtubules as well by a still poorly understood mechanism. in this study, we aim at deciphering the mechanisms by which the opportunistic bacterial pathogen pseudomonas aeruginosa invades nonphagocytic cells, although it is considered mainly an extracellular bacterium. using confocal microscopy and immuno ... | 2015 | 26037124 |
| the type vi secretion system modulates flagellar gene expression and secretion in citrobacter freundii and contributes to adhesion and cytotoxicity to host cells. | the type vi secretion system (t6ss) as a virulence factor-releasing system contributes to virulence development of various pathogens and is often activated upon contact with target cells. citrobacter freundii strain cf74 has a complete t6ss genomic island (gi) that contains clpv, hcp-2, and vgr t6ss genes. we constructed clpv, hcp-2, vgr, and t6ss gi deletion mutants in cf74 and analyzed their effects on the transcriptome overall and, specifically, on the flagellar system at the levels of transc ... | 2015 | 25870231 |
| synergistic antimicrobial activity of camellia sinensis and juglans regia against multidrug-resistant bacteria. | synergistic combinations of antimicrobial agents with different mechanisms of action have been introduced as more successful strategies to combat infections involving multidrug resistant (mdr) bacteria. in this study, we investigated synergistic antimicrobial activity of camellia sinensis and juglans regia which are commonly used plants with different antimicrobial agents. antimicrobial susceptibility of 350 gram-positive and gram-negative strains belonging to 10 different bacterial species, was ... | 2015 | 25719410 |
| analysis of the link between the redox state and enzymatic activity of the htra (degp) protein from escherichia coli. | bacterial htras are proteases engaged in extracytoplasmic activities during stressful conditions and pathogenesis. a model prokaryotic htra (htra/degp from escherichia coli) requires activation to cleave its substrates efficiently. in the inactive state of the enzyme, one of the regulatory loops, termed la, forms inhibitory contacts in the area of the active center. reduction of the disulfide bond located in the middle of la stimulates htra activity in vivo suggesting that this s-s bond may play ... | 2015 | 25710793 |
| species identification of strains belonging to genus citrobacter using the biochemical method and maldi-tof mass spectrometry. | strains of genus citrobacter (152 isolates from 1950 to 1988 deposited in the czech national collection of type cultures, prague) were re-classified using biological and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) methods. one-hundred thirty-six strains (ca. 90 %) were identified to the species level using the biological method with evaluation by farmer matrix. maldi-tof ms exhibited better identification capability, the data being more compact; th ... | 2015 | 25113540 |
| enrichment and identification of cellulolytic bacteria from the gastrointestinal tract of giant african snail, achatina fulica. | the cellulolytic bacterial community structure in gastrointestinal (gi) tract of achatina fulica was studied using culture-independent and -dependent methods by enrichment in carboxymethyl cellulose (cmc). culture-dependent method indicated that gi tract of snail was dominated by enterobacteriaceae members. when tested for cellulase activities, all isolates obtained by culture-dependent method showed both or either of cmcase or avicelase activity. isolate identified as citrobacter freundii showe ... | 2015 | 25432338 |
| exoelectrogenic bacterium phylogenetically related to citrobacter freundii, isolated from anodic biofilm of a microbial fuel cell. | an electrogenic bacterium, named citrobacter freundii z7, was isolated from the anodic biofilm of microbial fuel cell (mfc) inoculated with aerobic sewage sludge. cyclic voltammetry (cv) analysis exhibited that the strain z7 had relatively high electrochemical activity. when the strain z7 was inoculated into mfc, the maximum power density can reach 204.5 mw/m(2) using citrate as electron donor. series of substrates including glucose, glycerol, lactose, sucrose, and rhammose could be utilized to ... | 2015 | 25427595 |
| morphological alterations on citrobacter freundii bacteria induced by erythrosine dye and laser light. | the effect of the laser irradiation (532 nm) on films prepared from citrobacter freundii mixed with erythrosine dye was investigated by using atomic force microscopy. it was observed that morphological changes of bacterial surfaces after irradiations, which were attributed to cellular damage of the outer membranes, are a result of a photodynamic effect. the results suggested that the combination of erythrosine and laser light at 532 nm could be a candidate to a photodynamic therapy against c. fr ... | 2015 | 23975569 |
| inhibitory effects of lactobacillus fermentum on microbial growth and biofilm formation. | beneficial effects of lactobacilli have been reported, and lactic bacteria are employed for conservation of foods. therefore, the effects of a lactobacillus fermentum strain were analyzed regarding inhibitory effects on staphylococci, candida albicans and enterotoxigenic enterobacteria by transmission electron microscopy (tem). tem of bacterial biofilms was performed using cocultures of bacteriocin-producing l. fermentum 97 with different enterotoxigenic strains: staphylococcus epidermidis expre ... | 2015 | 26267163 |