Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| unique characteristics of pyogenic liver abscesses of biliary origin. | pyrogenic liver abscesses (pla) can be caused by cholangitis associated with hepatolithiasis and cholangitis related to an abnormal bilioenteric communication, such as a hepaticojejunostomy or the presence of indwelling biliary stents. our aim was to evaluate the clinical characteristics of pla of biliary origin. | 2016 | 26775071 |
| colistin resistance gene mcr-1 and phnshp45 plasmid in human isolates of escherichia coli and klebsiella pneumoniae. | 2016 | 26774239 | |
| antagonistic activity of nocardia brasiliensis ptcc 1422 against isolated enterobacteriaceae from urinary tract infections. | the main drawback of current antibiotic therapies is the emergence and rapid increase in antibiotic resistance. nocardiae are aerobic, gram-positive, catalase-positive, non-motile actinomycetes. nocardia brasiliensis was reported as antibiotic producer. the purpose of the study was to determine antibacterial activity of n. brasiliensis ptcc 1422 against isolated enterobacteriaceae from urinary tract infections (utis). the common bacteria from utis were isolated from hospital samples. antimicrobi ... | 2016 | 26920557 |
| gramicidin a mutants with antibiotic activity against both gram-positive and gram-negative bacteria. | antimicrobial peptides (amps) have shown potential as alternatives to traditional antibiotics for fighting infections caused by antibiotic-resistant bacteria. one promising example of this is gramicidin a (ga). in its wild-type sequence, ga is active by permeating the plasma membrane of gram-positive bacteria. however, ga is toxic to human red blood cells at similar concentrations to those required for it to exert its antimicrobial effects. installing cationic side chains into ga has been shown ... | 2016 | 26918268 |
| fast label-free detection of legionella spp. in biofilms by applying immunomagnetic beads and raman spectroscopy. | legionellae colonize biofilms, can form a biofilm by itself and multiply intracellularly within the protozoa commonly found in water distribution systems. approximately half of the known species are pathogenic and have been connected to severe multisystem legionnaires' disease. the detection methods for legionella spp. in water samples are still based on cultivation, which is time consuming due to the slow growth of this bacterium. here, we developed a cultivation-independent, label-free and fas ... | 2016 | 26915495 |
| predictability of phenotype in relation to common β-lactam resistance mechanisms in escherichia coli and klebsiella pneumoniae. | the minimal concentration of antibiotic required to inhibit the growth of different isolates of a given species with no acquired resistance mechanisms has a normal distribution. we have previously shown that the presence or absence of transmissible antibiotic resistance genes has excellent predictive power for phenotype. in this study, we analyzed the distribution of six β-lactam antibiotic susceptibility phenotypes associated with commonly acquired resistance genes in enterobacteriaceae in sydn ... | 2016 | 26912748 |
| clinico-microbiological study and antibiotic resistance profile of meca and esbl gene prevalence in patients with diabetic foot infections. | diabetic foot infections (dfis) constitute a major complication of diabetes mellitus. dfis contribute to the development of gangrene and non-traumatic lower extremity amputations with a lifetime risk of up to 25%. the aim of the present study was to identify the presence of neuropathy and determine the ulcer grade, microbial profile and phenotypic and genotypic prevalence of the methicillin-resistance gene meca and extended spectrum β-lactamase (esbl)-encoding genes in bacterial isolates of dfi ... | 2016 | 26998033 |
| curing vector for inci1 plasmids and its use to provide evidence for a metabolic burden of inci1 ctx-m-1 plasmid pifm3791 on klebsiella pneumoniae. | using a sequence-based approach we previously identified an inci1 ctx-m-1 plasmid, pifm3791, on a single pig farm in the uk that was harboured by klebsiella pneumoniae, escherichia coli and salmonella enterica serotype 4,5,12:i:-. to test the hypothesis that the plasmid had spread rapidly into these differing host bacteria we wished to assess whether the plasmid conferred a fitness advantage. to do this an inci1 curing vector was constructed and used to displace the inci1 ctx-m-1 plasmids from k ... | 2016 | 27166141 |
| in vitro activity of ceftaroline against bacterial pathogens isolated from skin and soft tissue infections in europe, russia and turkey in 2012: results from the assessing worldwide antimicrobial resistance evaluation (aware) surveillance programme. | the objective of this study was to analyse antimicrobial susceptibility testing data generated by the assessing worldwide antimicrobial resistance evaluation (aware) global surveillance programme for pathogens causing skin and soft tissue infections (sstis) in european countries in 2012. | 2016 | 26503667 |
| pediatric extended spectrum β-lactamase infection: community-acquired infection and treatment options. | infection caused by extended spectrum β-lactamase (esbl)-producing enterobacteriaceae in pediatric patients has been increasing and spreading to the community, compromising the options for effective antibiotics. this retrospective study was conducted to identify which antibiotics esbl-producing enterobacteriaceae remain susceptible to. in addition, the prevalence of community-acquired infection caused by these organisms, and the possibility of association between these organisms and septic shock ... | 2016 | 26513341 |
| detection and characterization of carbapenemase-producing enterobacteriaceae in wounded syrian patients admitted to hospitals in northern israel. | since 2013, four hospitals in northern israel have been providing care for syrian nationals, primarily those wounded in the ongoing civil war. we analyzed carbapenemase-producing enterobacteriaceae (cpe) isolates obtained from these patients. isolate identification was performed using the vitek 2 system. polymerase chain reaction (pcr) was performed for the presence of bla kpc, bla ndm, and bla oxa-48. susceptibility testing and genotyping were performed on selected isolates. during the study pe ... | 2016 | 26581423 |
| nosocomial bloodstream infections caused by escherichia coli and klebsiella pneumoniae resistant to third-generation cephalosporins, finland, 1999-2013: trends, patient characteristics and mortality. | few systematically collected multi-centre surveillance data on nosocomial bloodstream infections (bsi) caused by extended-spectrum β-lactamase (esbl) producing escherichia coli or klebsiella pneumoniae have been published. | 2016 | 26577519 |
| cefazolin loaded chitosan nanoparticles to cure multi drug resistant gram-negative pathogens. | antibiotic resistance against gram-negative microbes is considered as an alarming phenomenon that needs to be addressed urgently to develop better therapeutic solutions. the aim of the present research work was to investigate and develop cefazolin loaded chitosan nanoparticles (csnps) as a potential tool against multidrug resistant pathogens. empty and drug loaded csnps were prepared by ionic gelation method. it was observed by scanning electron microscopy (sem) and atomic force microscopy (afm) ... | 2016 | 26572401 |
| increasing incidence of extended-spectrum β-lactamase-producing escherichia coli in community hospitals throughout the southeastern united states. | objective to describe the epidemiology of extended-spectrum β-lactamase (esbl)-producing escherichia coli (esbl-ec) and klebsiella pneumoniae (esbl-kp) infections design retrospective cohort setting inpatient care at community hospitals patients all patients with esbl-ec or esbl-kp infections methods esbl-ec and esbl-kp infections from 26 community hospitals were prospectively entered into a centralized database from january 2009 to december 2014. results a total of 925 infections caused by esbl ... | 2016 | 26458226 |
| predicting abscesses in adults with community-onset monomicrobial enterobacteriaceae bacteremia: microorganisms matters. | enterobacteriaceae is a leading pathogen of community-onset bacteremia. this study aims to establish a predictive scoring algorithm to identify adults with community-onset enterobacteriaceae bacteremia who are at risk for abscesses. of the total 1262 adults, 152 (12.0%) with abscess occurrence were noted. the 6 risk factors significantly associated with abscess occurrence-liver cirrhosis, diabetes mellitus, thrombocytopenia and high c-reactive protein (>100 mg/l) at bacteremic onset, delayed def ... | 2016 | 26456388 |
| inhibition of recbcd in klebsiella pneumoniae by gam and its effect on the efficiency of gene replacement. | gam protein is an inhibitor of the host recbcd exonuclease, and this inhibition is essential to the proficiency of red recombinase-mediated gene replacement. in klebsiella pneumoniae, the efficiency of this gene replacement was lower than that in escherichia coli, and the minimum length of homologous extensions required was longer. thus, it was supposed that the inhibitory effect of gam against recbcd was weak in k. pneumoniae. to test this hypothesis, a gam-deficient red recombinase expression ... | 2016 | 26471352 |
| in vitro evaluation of dual carbapenem combinations against carbapenemase-producing enterobacteriaceae. | this study aimed to analyse the in vitro activity of dual combinations of carbapenems against klebsiella pneumoniae producing the main carbapenemase types. | 2016 | 26416781 |
| rapid molecular diagnostics, antibiotic treatment decisions, and developing approaches to inform empiric therapy: primers i and ii. | rapid molecular diagnostic (rmd) platforms may lead to better antibiotic use. our objective was to develop analytical strategies to enhance the interpretation of rmds for clinicians. | 2016 | 26409063 |
| impact of colistin sulfate treatment of broilers on the presence of resistant bacteria and resistance genes in stored or composted manure. | the application of manure may result in contamination of the environment with antimicrobials, antimicrobial-resistant bacteria, resistance genes and plasmids. the aim of this study was to investigate the impact of the administration of colistin and of manure management on (i) the presence of colistin-resistant escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa and (ii) the prevalence of various antimicrobial resistance genes in feces and in composted or stored manure. one flock o ... | 2016 | 26616601 |
| emergence of plasmid-mediated colistin resistance mechanism mcr-1 in animals and human beings in china: a microbiological and molecular biological study. | until now, polymyxin resistance has involved chromosomal mutations but has never been reported via horizontal gene transfer. during a routine surveillance project on antimicrobial resistance in commensal escherichia coli from food animals in china, a major increase of colistin resistance was observed. when an e coli strain, shp45, possessing colistin resistance that could be transferred to another strain, was isolated from a pig, we conducted further analysis of possible plasmid-mediated polymyx ... | 2016 | 26603172 |
| high prevalence and antimicrobial resistance of urinary tract infection isolates in febrile young children without localizing signs in taiwan. | antimicrobial susceptibility and prevalence of pediatric urinary tract infection (uti) is very useful for pediatricians in selecting effective antibiotics in time to improve outcomes in patients. this study aimed to determine the prevalence rate, bacterial distribution, and antimicrobial susceptibility of uti in febrile young children at a teaching hospital in northern taiwan. | 2016 | 26299351 |
| comparative in vitro activity of oral antimicrobial agents against enterobacteriaceae from patients with community-acquired urinary tract infections in three european countries. | enterobacteriaceae causing community-acquired urinary tract infections were examined in selected outpatient clinics and hospitals in belgium, germany and spain using eucast breakpoints for susceptibility. a total of 1190 isolates were collected. escherichia coli isolates were resistant to amoxicillin-clavulanic acid (28.1%), ciprofloxacin (23.4%) and trimethoprim-sulfamethoxazole (21.4%) compared with fosfomycin and nitrofurantoin (each, <1.5%). ceftibuten (mic50/90 0.25/0.5 mg/l) and ceftriaxon ... | 2016 | 26321667 |
| ndm-producing enterobacteriaceae in poland, 2012-14: inter-regional outbreak of klebsiella pneumoniae st11 and sporadic cases. | the objective of this study was to characterize new delhi metallo-β-lactamase (ndm)-producing enterobacteriaceae isolates reported in poland in 2012-14. | 2016 | 26386745 |
| mass gathering medicine (hajj pilgrimage in saudi arabia): the clinical pattern of pneumonia among pilgrims during hajj. | the planned annual hajj to the holy shrines in makkah, saudi arabia, is recognized as one of the largest recurring religious mass gatherings globally, and the outbreak of infectious diseases is of major concern. we aim to study the incidence, etiology, risk factors, length of hospital stay, and mortality rate of pneumonia amongst pilgrims admitted to al-ansar general hospital, madinah, saudi arabia during the hajj period of december 2004-november 2013. a retrospective analysis of all patients di ... | 2016 | 27262693 |
| scotti: efficient reconstruction of transmission within outbreaks with the structured coalescent. | exploiting pathogen genomes to reconstruct transmission represents a powerful tool in the fight against infectious disease. however, their interpretation rests on a number of simplifying assumptions that regularly ignore important complexities of real data, in particular within-host evolution and non-sampled patients. here we propose a new approach to transmission inference called scotti (structured coalescent transmission tree inference). this method is based on a statistical framework that mod ... | 2016 | 27681228 |
| etiology of early onset septicemia among neonates at the university college hospital, ibadan, nigeria. | neonatal septicemia remains a major cause of newborn deaths in developing countries. its burden is further compounded by the emergence of multidrug-resistant pathogens, which is related to a lack of antibiotic protocols resulting in unrestricted use of antibiotics. the absence of reliable antibiotic sensitivity testing makes the formulation of antibiotic guidelines and judicious use of antibiotics difficult. this study sought to identify the current bacterial agents associated with early onset s ... | 2016 | 28036314 |
| in vivo acquisition of carbapenemase gene blakpc-2 in multiple species of enterobacteriaceae through horizontal transfer of insertion sequence or plasmid. | objectives: current worldwide spread of carbapenem resistance in enterobacteriaceae constitutes a critical public health threat. this study aims to investigate how carbapenem resistance is acquired in enterobacteriaceae in patients during antimicrobial therapy. methods: clinical strains from the same anatomical site of the same patients that converted from carbapenem-susceptible to resistant during antimicrobial therapy and showed identical or similar pfge patterns were identified. the similarly ... | 2016 | 27818649 |
| bioprospecting of lipolytic microorganisms obtained from industrial effluents. | the lipases have ability to catalyze diverse reactions and are important in different biotechnological applications. the aim of this work was to isolate and characterize microorganisms that produce lipases, from different food industry effluents localized in pelotas, rs/brazil. bacteria were identified using gram stain and biochemical tests (vitek 2(r)). fungi were identified according to macro and micromorphology characteristics. the extracellular lipase production was evaluated using the rhoda ... | 2016 | 27556331 |
| physiological and molecular characteristics of carbapenem resistance in klebsiella pneumoniae and enterobacter aerogenes. | bacterial resistance is a growing concern in the nosocomial environment in which klebsiella pneumoniae and enterobacter aerogenes play an important role due to their opportunism and carbapenemase-production. this work aimed to evaluate physiological and molecular characteristics of carbapenem-resistant k. pneumoniae and e. aerogenes isolated in a brazilian tertiary hospital. | 2016 | 27367007 |
| isolation of kpc 3-producing enterobacter aerogenes in a patient colonized by mdr klebsiella pneumoniae. | we describe the interspecies transmission of the plasmid-mediated blakpc-3 gene, which confers carbapenem resistance, between clinically relevant gram-negative bacteria in a single patient. a kpc-3 producing enterobacter aerogenes was isolated from a hospitalized patient previously colonized and then infected by a klebsiella pneumoniae st101 carrying the blakpc-3 gene. the strains showed identical plasmids. since intense horizontal exchanges among bacteria can occur in the gut, clinicians should ... | 2016 | 27284988 |
| occurrence and analysis of irp2 virulence gene in isolates of klebsiella pneumoniae and enterobacter spp. from microbiota and hospital and community-acquired infections. | eighty-five isolates of klebsiella pneumoniae and enterobacter spp., originating from hospital- and community-acquired infections and from oropharyngeal and faecal microbiota from patients in recife-pe, brazil, were analyzed regarding the presence of irp2 gene. this is a yersinia typical gene involved in the synthesis of siderophore yersiniabactin. dna sequencing confirmed the identity of irp2 gene in five k. pneumoniae, five enterobacter aerogenes and one enterobacter amnigenus isolates. to our ... | 2016 | 27133266 |
| multidrug-resistant combined infections in a liver transplanted patient: case report. | we report a case of successfully treated multiple liver abscesses in a liver-transplanted patient, sustained by combined multidrug-resistant infections. two months after a liver transplant, a computed tomography scan revealed the presence of multiple abscesses in the liver graft. blood cultures and abscessual liver fluid were both positive for acquired colistin- and carbapenem- resistant klebsiella pneumoniae and an extended-spectrum of beta-lactamases-producing enterobacter aerogenes. the treat ... | 2016 | 27063638 |
| isolation of enterobacter aerogenes carrying blatem-1 and blakpc-3 genes recovered from a hospital intensive care unit. | enterobacter aerogenes has recently emerged as an important hospital pathogen. in this study, we showed the emergence of e. aerogenes isolates carrying the blakpc gene in patients colonized by carbapenem-resistant klebsiella pneumoniae strains. two multiresistant e. aerogenes isolates were recovered from bronchial aspirates of two patients hospitalized in the intensive care unit at the "santa maria della scaletta" hospital, imola. the antimicrobial susceptibility test showed the high resistance ... | 2016 | 27004836 |
| unusual indolent course of a chronic active epstein-barr virus-associated natural killer cell lymphoproliferative disorder. | natural killer (nk) cell lymphoproliferative disorders are uncommon and the epstein-barr virus (ebv) plays an important aetiological role in their pathogenesis. we report a 20-year-old male with a chronic active ebv infection associated with a nk cell lymphoproliferative disorder which had an unusual indolent course. he presented to the sultan qaboos university hospital in muscat, oman, in december 2011 with a history of intermittent fever and coughing. examinations revealed generalised lymphade ... | 2016 | 27226916 |
| biological activities of some acacia spp. (fabaceae) against new clinical isolates identified by ribosomal rna gene-based phylogenetic analysis. | nowadays,most of the pathogenic bacteria become resistant to antibiotics. therefore,the pharmaceutical properties of the natural plant extracts have become of interest to researchers as alternative antimicrobial agents. in this study,antibacterial activities of extract gained from acacia etbaica, acacia laeta, acacia origena and acacia pycnantha have been evaluated against isolated pathogenic bacteria (strains mfm-01, mfm-10 and ah-09) using agar well diffusion methods.the bacterial strains were ... | 2016 | 26826814 |
| long-term outbreak of klebsiella pneumoniae& third generation cephalosporin use in a neonatal intensive care unit in north india. | the indiscriminate use of third generation cephalosporin has contributed to the emergence and widespread dissemination of extended spectrum β lactamases (esbl) genes in klebsiella pneumoniae. this study was undertaken to elaborate the genetic behaviour of esbl - producing k. pneumoniae isolates in the neonatal intensive care unit (nicu) of a tertiary care hospital in north india causing successive outbreaks in context with empirical third generation cephalosporin use. | 2016 | 28256474 |
| the diversity of klebsiella pneumoniae surface polysaccharides. | klebsiella pneumoniae is considered an urgent health concern due to the emergence of multi-drug-resistant strains for which vaccination offers a potential remedy. vaccines based on surface polysaccharides are highly promising but need to address the high diversity of surface-exposed polysaccharides, synthesized as o-antigens (lipopolysaccharide, lps) and k-antigens (capsule polysaccharide, cps), present in k. pneumoniae. we present a comprehensive and clinically relevant study of the diversity o ... | 2016 | 28348868 |
| identification of klebsiella capsule synthesis loci from whole genome data. | klebsiella pneumoniae is a growing cause of healthcare-associated infections for which multi-drug resistance is a concern. its polysaccharide capsule is a major virulence determinant and epidemiological marker. however, little is known about capsule epidemiology since serological typing is not widely accessible and many isolates are serologically non-typeable. molecular typing techniques provide useful insights, but existing methods fail to take full advantage of the information in whole genome ... | 2016 | 28348840 |
| prevalence of two multidrug-resistant klebsiella species in an indian teaching hospital and adjoining community. | the gram-negative pathogenic bacteria klebsiella oxytoca and klebsiella pneumoniae produce the extended spectrum β-lactamase (esbl) and cephalosporinase enzymes and are the major causes of hospital acquired (ha) infections and epidemics in non-hygienic communities in the majority of developing countries. | 2016 | 24996691 |
| molecular mechanisms of colistin resistance in klebsiella pneumoniae causing bacteremia from india-a first report. | colistin has long been a reserve drug used for the treatment of carbapenem resistant klebsiella pneumoniae. carbapenem resistance in k. pneumoniae has been increasing and is as high as 44% in india. although a reserve agent, with rise in rates of resistance to carbapenems, the usage of colistin has increased over the years leading to slow emergence of resistance. colistin resistance is mainly mediated by the alteration in the lps of bacterial outer membrane with the addition of l-ara4-n and petn ... | 2016 | 28119670 |
| face and/or neck burns: a risk factor for respiratory infection? | infections are a common cause of morbidity and mortality in burn patients, and almost 50% of infection-related deaths in burn patients are caused by pneumonia. the proportion of facial and/or neck burns (fnb) in burn centre admissions is high, and these patients have a well known respiratory risk. however, it is not well established in the literature if the occurrence of a fnb is a risk factor for respiratory infection during hospitalization. a retrospective, single-centre trial at the burn unit ... | 2016 | 28149229 |
| approach to carbapenemase detection in klebsiella pneumoniae in routine diagnostic laboratories. | resistance to carbapenems in klebsiella may be due to carbapenem hydrolysing enzymes. accurate detection of carbapenemase must be done for patient treatment and epidemiological purposes. | 2016 | 28208858 |
| [clonal relationship and detection of blakpc gene in strains of klebsiella pneumoniae resistant to carbapenems, at a hospital in venezuela]. | in order to study the clonal relationship and blakpc gene detection in clinical isolates of klebsiella pneumoniae resistant to carbapenems, we analyzed 22 clinical isolates of k. pneumoniae with resistance to imipenem and/ or meropenem, isolated in the laboratory of bacteriology at the university hospital "antonio patricio de alcalá" (huapa) from the cumana city, sucre state, venezuela, for a period of five consecutive years. susceptibility to different antimicrobials was determined, and the pre ... | 2016 | 28112335 |
| probiotic properties of lactobacillus fermentum strains isolated from human oral samples and description of their antibacterial activity. | gram positive bacteria produce peptides, defined bacteriocins which exhibit good antibacterial activity. | 2016 | 28034294 |
| genome sequence of kp-rio/2015, a novel klebsiella pneumoniae (podoviridae) phage. | klebsiella pneumoniae is a pathogen frequently associated with antibiotic-resistant nosocomial infections. here, we describe the genome of kp-rio/2015, a novel phage of k. pneumoniae belonging to the family podoviridae. | 2016 | 28007847 |
| molecular typing and virulence analysis of multidrug resistant klebsiella pneumoniae clinical isolates recovered from egyptian hospitals. | klebsiella pneumonia infection rates have increased dramatically. molecular typing and virulence analysis are powerful tools that can shed light on klebsiella pneumonia infections. whereas 77.7% (28/36) of clinical isolates indicated multidrug resistant (mdr) patterns, 50% (18/36) indicated carpabenem resistance. gene prevalence for the acrab efflux pump (82.14%) was more than that of the mdtk efflux pump (32.14%) in the mdr isolates. fimh-1 and mrkd genes were prevalent in wound and blood isola ... | 2016 | 28004732 |
| klebsiella pneumoniae planktonic and biofilm reduction by different plant extracts: in vitro study. | this study evaluated the action of pfaffia paniculata k., juglans regia l., and rosmarius officinalis l. extracts against planktonic form and biofilm of klebsiella pneumoniae (atcc 4352). minimum inhibitory concentration (mic) and minimum microbicidal concentration (mmc) values were determined for each extract by microdilution broth method, according to clinical and laboratory standards institute. next, antimicrobial activity of the extracts on biofilm was analyzed. for this, standardized suspen ... | 2016 | 28004034 |
| a rapid spin column-based method to enrich pathogen transcripts from eukaryotic host cells prior to sequencing. | when analyzing pathogen transcriptomes during the infection of host cells, the signal-to-background (pathogen-to-host) ratio of nucleic acids (na) in infected samples is very small. despite the advancements in next-generation sequencing, the minute amount of pathogen na makes standard rna-seq library preps inadequate for effective gene-level analysis of the pathogen in cases with low bacterial loads. in order to provide a more complete picture of the pathogen transcriptome during an infection, w ... | 2016 | 28002481 |
| klebsiella pneumoniae of suspected human origin from free-living common seals on the east coast of england. | 2016 | 27993969 | |
| epidemiology and biomolecular characterization of carbapenem-resistant klebsiella pneumoniae in an italian hospital. | to describe the occurrence of crkp infections in a tertiary care hospital and to analyse the allelic profiles of the clinical strains involved and the most frequent carbapenemases. | 2016 | 27980379 |
| genome sequences of multidrug-resistant, colistin-susceptible and -resistant klebsiella pneumoniae clinical isolates from pakistan. | the emergence and spread of colistin resistance among multidrug-resistant (mdr) klebsiella pneumoniae represent a critical threat to global health. here, we report the complete genome sequences of 10 mdr, colistin-susceptible and -resistant k. pneumoniae clinical isolates obtained in pakistan between 2010 and 2013. | 2016 | 27979956 |
| complete genome sequence of community-acquired klebsiella pneumoniae kp36, a strain isolated from a patient with an upper urinary tract infection. | here, we announce the complete genome sequence of klebsiella pneumoniae kp36, a strain isolated from a patient with a severe community-acquired urinary tract infection. this genome provides insights into the pathogenesis of a pandemic k. pneumoniae strain from a community-acquired urinary tract infection. | 2016 | 27979951 |
| high-throughput and automated diagnosis of antimicrobial resistance using a cost-effective cellphone-based micro-plate reader. | routine antimicrobial susceptibility testing (ast) can prevent deaths due to bacteria and reduce the spread of multi-drug-resistance, but cannot be regularly performed in resource-limited-settings due to technological challenges, high-costs, and lack of trained professionals. we demonstrate an automated and cost-effective cellphone-based 96-well microtiter-plate (mtp) reader, capable of performing ast without the need for trained diagnosticians. our system includes a 3d-printed smartphone attach ... | 2016 | 27976700 |
| multiple variants of klebsiella pneumoniae producing carbapenemase in one patient. | 2016 | 27974041 | |
| multiple-locus variable number tandem repeat analysis of klebsiella pneumoniae: comparison with pulsed-field gel electrophoresis. | to assess whether multiple-locus variable-number tandem repeat analysis (mlva) could replace pulsed-field gel electrophoresis (pfge) for genotyping of klebsiella pneumoniae, this study was conducted to compare the typeability, discriminatory power, and concordance of these methods. | 2016 | 27960064 |
| ndm-1 and rmtc-producing klebsiella pneumoniae isolates in turkey. | the resistance of aminoglycosides in strains that produce beta-lactamase can be developed through the multidrug resistant encoding genes carried by common plasmids. recently, the association between 16s rrna methyltransferase resistance and beta-lactamase enzymes carried by the same plasmids has drawn increased attention from researchers, particularly the association in aminoglycoside-resistant strains with a minimum inhibitory concentration (mic) of ≥ 256 µg/ml. | 2016 | 27942358 |
| assessment of antibiotic resistance in klebsiella pneumoniae exposed to sequential in vitro antibiotic treatments. | bacteria treated with different classes of antibiotics exhibit changes in susceptibility to successive antibiotic treatments. this study was designed to evaluate the influence of sequential antibiotic treatments on the development of antibiotic resistance in klebsiella pneumoniae associated with β-lactamase and efflux pump activities. | 2016 | 27938381 |
| molecular identification of extended-spectrum β-lactamase and integron genes in klebsiella pneumonia. | infections caused by gram negative bacteria, producing extended-spectrum β-lactamase, including klebsiella pneumoniae are increasing all over the world with high morbidity and mortality. the aim of the present study was determined antimicrobial profile susceptibility and the prevalence of antibiotic resistance genes by multiplex pcr. | 2016 | 27935927 |
| siderophores: more than stealing iron. | siderophores are small molecular iron chelators that are produced by microbes and whose most notable function is to sequester iron from the host and provide this essential metal nutrient to microbes. recent studies have proposed additional, noncanonical roles for siderophores, including the acquisition of noniron metals and modulation of host functions. recently, holden et al. (v. i. holden, p. breen, s. houle, c. m. dozois, and m. a. bachman, mbio 7:e01397-16, 2016, http://dx.doi.org/10.1128/mb ... | 2016 | 27935843 |
| incidence, risk factors and associated mortality of central line-associated bloodstream infections at an intensive care unit in northern india. | to evaluate the incidence, risk factors and associated mortality of central line-associated bloodstream infection (clabsi) in an adult intensive care unit (icu) in india. | 2016 | 27940521 |
| emergence of serotype k1 klebsiella pneumoniae st23 strains co-producing the plasmid-mediated ampc beta-lactamase dha-1 and an extended-spectrum beta-lactamase in korea. | serotype k1 klebsiella pneumoniae has emerged as an important community pathogen causing various infections, including liver abscesses. although serotype k1 k. pneumoniae community isolates have been reported as susceptible to most classes of antimicrobial agents, a few cases of infection caused by extended-spectrum beta-lactamase (esbl)-producing serotype k1 k. pneumoniae have recently been reported in asian countries. we identified three esbl-producing strains of serotype k1 k. pneumoniae and ... | 2016 | 27933141 |
| draft genome sequences of three hypervirulent carbapenem-resistant klebsiella pneumoniae isolates from bacteremia. | hypervirulent klebsiella pneumoniae strains have been increasingly reported worldwide, and there is emergence of carbapenem resistance among them. here, we report the genome sequences of three carbapenem-resistant hypervirulent k. pneumoniae isolates isolated from bacteremic patients at a tertiary-care center in south india. | 2016 | 27932638 |
| il-17 receptor signaling in the lung epithelium is required for mucosal chemokine gradients and pulmonary host defense against k. pneumoniae. | the cytokine il-17, and signaling via its heterodimeric il-17ra/il-17rc receptor, is critical for host defense against extracellular bacterial and fungal pathogens. polarized lung epithelial cells express il-17ra and il-17rc basolaterally. however, their contribution to il-17-dependent pulmonary defenses in vivo remains to be determined. to address this, we generated mice with conditional deletion of il17ra or il17rc in scgb1a1-expressing club cells, a major component of the murine bronchiolar e ... | 2016 | 27923703 |
| community-acquired meningitis caused by a cg86 hypervirulent klebsiella pneumoniae strain: first case report in the caribbean. | community-acquired bacterial meningitis due to klebsiella pneumoniae has mainly been described in southeast asia and has a poor prognosis. severe invasive infections caused by k. pneumoniae, including meningitis, are often due to hypervirulent strains (hvkp), which are characterized by capsular serotypes k1 and k2, a gene responsible for hypermucoviscosity, and the cluster for synthesis of the siderophore aerobactin. | 2016 | 27923372 |
| feasibility of radiofrequency ablation as an alternative to surgical intervention in patients with huge multiloculated pyogenic liver abscesses: a retrospective cohort study. | this retrospective cohort study investigated the feasibility of radiofrequency (rf) ablation as an alternative to surgical intervention in patients with huge multiloculated pyogenic liver abscesses (plas).from august 2010 to april 2016, 83 patients with pla were admitted to beijing chao-yang hospital, china. four of these patients had huge multiloculated plas and underwent rf ablation plus antibiotics. the inclusion criteria for rf ablation were as follows: multiloculated pla confirmed by comput ... | 2016 | 27930527 |
| the crystal structure of the endoglucanase cel10, a family 8 glycosyl hydrolase from klebsiella pneumoniae. | cellulases are produced by microorganisms that grow on cellulose biomass. here, a cellulase, cel10, was identified in a strain of klebsiella pneumoniae isolated from chinese bamboo rat gut. analysis of substrate specificity showed that cel10 is able to hydrolyze amorphous carboxymethyl cellulose (cmc) and crystalline forms of cellulose (avicel and xylan) but is unable to hydrolyze p-nitrophenol β-d-glucopyranoside (p-npg), proving that cel10 is an endoglucanase. a phylogenetic tree analysis indi ... | 2016 | 27917834 |
| synergistic effect of thymbra spicata l. extracts with antibiotics against multidrug- resistant staphylococcus aureus and klebsiella pneumoniae strains. | to evaluate the in vitro interaction between different extracts of thymbra spicata l. and certain antimicrobial drugs of different mechanisms, including ampicillin, cefotaxime, amikacin and ciprofloxacin. this study was performed against multidrug-resistant strains of staphylococcus aureus and klebsiella pneumoniae. | 2016 | 27917275 |
| capsule-targeting depolymerase, derived from klebsiella kp36 phage, as a tool for the development of anti-virulent strategy. | the rise of antibiotic-resistant klebsiella pneumoniae, a leading nosocomial pathogen, prompts the need for alternative therapies. we have identified and characterized a novel depolymerase enzyme encoded by klebsiella phage kp36 (depokp36), from the siphoviridae family. to gain insights into the catalytic and structural features of depokp36, we have recombinantly produced this protein of 93.4 kda and showed that it is able to hydrolyze a crude exopolysaccharide of a k. pneumoniae host. using in ... | 2016 | 27916936 |
| primary liver abscess caused by klebsiella pneumoniae: an increasingly common entity. | 2016 | 27914678 | |
| central-line associated bloodstream infections in a tertiary care children's university hospital: a prospective study. | the central-line associated bloodstream infections (clabsi) are the most common healthcare-associated infections in childhood. despite the international data available on healthcare-associated infections in selected groups of patients, there is a lack of large and good quality studies. the present survey is the first prospective study monitoring for 6 months the occurrence of central-line associated bloodstream infections in all departments of an italian tertiary care children's university hospi ... | 2016 | 27903240 |
| ultrastructural changes caused by polymyxin b and meropenem in multiresistant klebsiella pneumoniae carrying blakpc-2 gene. | the ultrastructural alterations caused by polymyxin b and meropenem and by the association between polymyxin b and meropenem were investigated in two multiresistant isolates of klebsiella pneumoniae (k3-a2 and k12-a2) carriers of blakpc-2, coming from infection and colonization in patients in a public hospital in recife, brazil. the ultrastructural changes were detected by transmission electron microscopy and scanning. the susceptibility of the isolates to antimicrobials was tested by the disc d ... | 2016 | 27902404 |
| epidemiology of klebsiella pneumoniae bloodstream infections in a teaching hospital: factors related to the carbapenem resistance and patient mortality. | although klebsiella pneumoniae bloodstream infections (kp-bsis) have recently attracted attention due to an alarming raise in morbidity and mortality, there have been few reports on the epidemiology of kp-bsis in mainland china. we sought to describe the epidemiological, microbiological, and clinical characteristics of kp-bsis, focusing on the risk factors of carbapenem resistance and patient mortality. | 2016 | 27891222 |
| evaluating the effect of oxygen concentrations on antibiotic sensitivity, growth, and biofilm formation of human pathogens. | standard antimicrobial susceptibility tests are performed in vitro under normal room oxygen conditions to predict the in vivo effectiveness of antimicrobial therapy. the aim of this study was to conduct a comprehensive analysis of the effect of different oxygen levels on the antibiotic susceptibility of two strains of staphylococcus aureus, pseudomonas aeruginosa, and klebsiella pneumoniae. it was found that anoxic conditions caused reduced sensitivity of bacteria to aminoglycoside antibiotics i ... | 2016 | 27891050 |
| coexistence of fosfomycin and colistin resistance in klebsiella pneumoniae: whole-genome shotgun sequencing. | resistance to colistin is a major threat that limits therapeutic choices for treating carbapenem-resistant klebsiella pneumoniae infections. herein, we report the draft genome sequences of two colistin-resistant k. pneumoniae isolates (ba41763 and b6753). the sequence data indicate that ba41763 and b6753 contain genomes of ~5.9 and 5.7 mb in size with several plasmids. | 2016 | 27881539 |
| ereply. diagnostic intricacies and fortuitous treatment approaches for carbapenem-resistant klebsiella pneumoniae. | 2016 | 27880708 | |
| ecomment. diagnostic intricacies and fortuitous treatment approaches for carbapenem-resistant klebsiella pneumoniae. | 2016 | 27880707 | |
| successful elimination of extended-spectrum beta-lactamase (esbl)-producing nosocomial bacteria at a neonatal intensive care unit. | extended-spectrum beta-lactamase (esbl)-producing gram-negative bacteria are highly dangerous to neonates. at our neonatal intensive care unit (nicu), the presence of these bacteria became so threatening in 2011 that immediate intervention was required. | 2016 | 27878781 |
| [multiple organ failure by serotype k1 klebsiella pneumoniae]. | 2016 | 27876187 | |
| high prevalence of esbl-producing klebsiella pneumoniae causing community-onset infections in china. | the aim of this work was to investigate the epidemiological and genetic characteristics of esbl-producing klebsiella pneumoniae (esbl-kp) causing community-onset infections. k. pneumoniae isolates were collected from 31 chinese secondary hospitals between august 2010 and 2011. genes encoding esbl and ampc beta-lactamases were detected by pcr. the isolates were assigned to sequence types (sts) using multi-locus sequence typing (mlst). eleven esbl-kp strains were selected for whole-genome sequenci ... | 2016 | 27895637 |
| a prospective, observational study comparing the pk/pd relationships of generic meropenem (mercide(®)) to the innovator brand in critically ill patients. | clinicians' skepticism, fueled by evidence of inferiority of some multisource generic antimicrobial products, results in the underutilization of more cost-effective generics, especially in critically ill patients. the aim of this observational study was to demonstrate equivalence between the generic or comparator brand of meropenem (mercide(®)) and the leading innovator brand (meronem(®)) by means of an ex vivo technique whereby antimicrobial activity is used to estimate plasma concentration of ... | 2016 | 27895516 |
| occurrence of diverse antimicrobial resistance determinants in genetically unrelated biocide tolerant klebsiella pneumoniae. | nosocomial infections due to klebsiella pneumoniae is a significant problem in health care settings worldwide. in this study, we examined the antimicrobial susceptibility, genetic profiles and mechanisms of antibiotic resistance in k. pneumoniae isolates of indian origin. to our knowledge this is the first report demonstrating the high prevalence of β-lactamases, aminoglycoside modifying enzymes, quinolone resistance genes besides demonstrating the involvement of active efflux in k. pneumoniae i ... | 2016 | 27870879 |
| synthesis of silver nanoparticles by endosymbiont pseudomonas fluorescens ca 417 and their bactericidal activity. | the present study emphasizes on biogenic synthesis of silver nanoparticles and their bactericidal activity against human and phytopathogens. nanoparticle synthesis was performed using endosymbiont pseudomonas fluorescens ca 417 inhabiting coffea arabica l. synthesized nanoparticles were characterized using hyphenated spectroscopic techniques such as uv-vis spectroscopy which revealed maximum absorption 425nm. fourier transform infrared spectroscopy (ftir) analysis revealed the possible functiona ... | 2016 | 27866607 |
| two unusual cases of successful treatment of hypermucoviscous klebsiella pneumoniae invasive syndrome. | a few japanese cases of hypermucoviscous klebsiella pneumoniae (k. pneumoniae) invasive syndrome have recently been reported. although extrahepatic complications from bacteremic dissemination have been observed, infected aneurysms are rare. furthermore, the primary source of infection is generally a liver abscess, and is rarely the prostate. therefore, we report two atypical cases of hypermucoviscous k. pneumoniae invasive syndrome. | 2016 | 27852233 |
| mortality markers in nosocomial klebsiella pneumoniae bloodstream infection. | klebsiella pneumoniae is the most common endogen agent for nosocomial infections. in this study, mortality markers were investigated in patients with nosocomial k. pneumoniae blood stream infection (nkp bsi). | 2016 | 27843749 |
| characterisation of carbapenem-resistant enterobacteriaceae from the southwestern ohio, northern kentucky and southeastern indiana region. | although carbapenem-resistant enterobacteriaceae (cre) have been recognised worldwide, there are important gaps in regional prevalence data. this study sought to determine the occurrence and type of cre in the cincinnati tri-state region. clinical microbiology laboratories in the region screened patient stool and rectal swab samples using chromogenic screening agar. isolates of enterobacteriaceae resistant to carbapenems were characterised using molecular methods. area institutions screened 1939 ... | 2016 | 27835839 |
| draft genome sequence of an extended-spectrum-β-lactamase-positive hypervirulent klebsiella pneumoniae strain with novel sequence type 2318 isolated from a neonate. | antimicrobial resistance among hypervirulent klebsiella pneumoniae is increasingly reported. here, we report the draft genome sequence of a hypervirulent k. pneumoniae strain isolated from a neonate with sepsis belonging to novel sequence type 2318 (st2318). | 2016 | 27834717 |
| predatory bacteria attenuate klebsiella pneumoniae burden in rat lungs. | bdellovibrio bacteriovorus and micavibrio aeruginosavorus are predatory bacteria that naturally-and obligately-prey on other gram-negative bacteria, and their use has been proposed as a potential new approach to control microbial infection. the ability of predatory bacteria to prey on gram-negative human pathogens in vitro is well documented; however, the in vivo safety and efficacy of predatory bacteria have yet to be fully assessed. in this study, we examined whether predatory bacteria can red ... | 2016 | 27834203 |
| the antimicrobial susceptibility of klebsiella pneumoniae from community settings in taiwan, a trend analysis. | drug-resistant klebsiella pneumoniae, especially extended-spectrum β-lactamase (esbl)- and/or ampc β-lactamase-producing strains, is an emerging problem worldwide. however, few data focusing on drug susceptibility of k. pneumoniae from community is available. in this study, we analyzed 1016 k. pneumoniae isolates from outpatients or those visiting emergency rooms collected during 2002-2012 from taiwan surveillance of antimicrobial resistance program. significantly decreased susceptibilities to 3 ... | 2016 | 27824151 |
| fatal donor-derived carbapenem-resistant klebsiella pneumoniae infection in a combined kidney-pancreas transplantation. | carbapenem-resistant klebsiella pneumoniae (cr-kp) infections in solid organ transplant recipients are associated with high morbidity and mortality. we report a case of a fatal donor-derived cr-kp infection in a combined kidney-pancreas transplant. given the short interval of time between donor hospitalization and organ procurement, information concerning the donor cr-kp positivity arrived only 72 hours after transplant. based on this experience, we believe that knowledge of the donor's cr-kp st ... | 2016 | 27822401 |
| klebsiella pneumoniae blakpc-3 nosocomial epidemic: bayesian and evolutionary analysis. | k. pneumoniae isolates carrying blakpc-3 gene were collected to perform bayesian phylogenetic and selective pressure analysis and to apply homology modeling to the kpc-3 protein. a dataset of 44 blakpc-3 gene sequences from clinical isolates of k. pneumoniae was used for bayesian phylogenetic, selective pressure analysis and homology modeling. the mean evolutionary rate for blakpc-3 gene was 2.67×10(-3) substitution/site/year (95% hpd: 3.4×10(-4-)5.59×10(-)(3)). the root of the bayesian tree dat ... | 2016 | 27815135 |
| draft genome sequence of a hospital-associated clone of klebsiella pneumoniae st340/cc258 coproducing rmtg and kpc-2 isolated from a pediatric patient. | we report here the draft genome sequence of a klebsiella pneumoniae strain 1194/11, belonging to the hospital-associated sequence type 340 (st340; clonal complex cc258), isolated from a catheter tip culture from a pediatric patient. the multidrug-resistant strain coproduced the 16s rrna methyltransferase rrna rmtg and β-lactamases kpc-2 and ctx-m-15. | 2016 | 27811089 |
| a self-quenching-resistant carbon nanodot powder with multicolored solid-state fluorescence for ultra-fast staining of various representative bacterial species within one minute. | in this study, we prepared self-quenching-resistant solid-state fluorescent carbon nanodots (sfcds) without any other solid matrices. the sfcds were prepared using a one-step microwave synthesis method through precise control of the heating power and time. the resulting sfcd powder showed excitation-dependent emission behavior with a maximum fluorescence quantum yield of 40%. the multicolored sfcds were successfully used as fluorescent agents for rapid staining of 14 representative bacterial spe ... | 2016 | 27874136 |
| molecular characterization and analysis of antimicrobial activity of endophytic fungi from medicinal plants in saudi arabia. | endophytic fungi, which have been reported in numerous plant species, are important components of the forest community and contribute significantly to the diversity of natural ecosystems. | 2016 | 27099679 |
| mechanism for the primary transformation of acetaminophen in a soil/water system. | the transformation of acetaminophen (apap) in a soil/water system was systematically investigated by a combination of kinetic studies and a quantitative analysis of the reaction intermediates. biotransformation was the predominant pathway for the elimination of apap, whereas hydrolysis or other chemical transformation, and adsorption processes made almost no contribution to the transformation under a dark incubation. bacillus aryabhattai strain 1-sj-5-2-5-m, klebsiella pneumoniae strain s001, an ... | 2016 | 27107139 |
| in vitro antimicrobial activities of different solvent extracted samples from iris germinica. | antibacterial and antifungal activities of different solvents extracted samples of iris germinica were carried out through disc diffusion assay. for this purpose five different solvent extracts were prepared with two concentrations (1 and 2 mg disc(-1)) and their antimicrobial activity was tested using disc diffusion assay against eight pathogenic bacteria viz. staphylococcus aureus, b. subtilis, bacillus atrophaeus, escherichia coli, pseudomonas aeruginosa, erwinia carotovara, klebsiella pneumo ... | 2016 | 26826828 |
| bacteriocinogenic bacteria isolated from raw goat milk and goat cheese produced in the center of méxico. | currently, there are few reports on the isolation of microorganisms from goat milk and goat cheese that have antibacterial activity. in particular, there are no reports on the isolation of microorganisms with antibacterial activity from these products in central mexico. our objective was to isolate bacteria, from goat products, that synthesized antimicrobial peptides with activity against a variety of clinically significant bacteria. we isolated and identified lactobacillus rhamnosus, l. plantar ... | 2016 | 27407294 |
| modulation of phytoremediation and plant growth by the treatment with pgpr, ag nanoparticle and untreated municipal wastewater. | the present attempt was made to determine the effects of untreated municipal wastewater (mw) on growth and physiology of maize and to evaluate the role of ag nanoparticle and plant-growth-promoting rhizobacteria (pgpr) when interacting with mw used for irrigation. it was used for the isolation of pgpr. the isolates were identified and characterized based on the colony morphology, c/n source utilization pattern using miniaturized identification system (qts 24), catalase (cat) and oxidase tests, a ... | 2016 | 27348506 |
| new coumarin derivatives from ferula pseudalliacea with antibacterial activity. | in this study, the antibacterial activity of disesquiterpene coumarin and sesquiterpene coumarins obtained from ferula pseudalliacea roots was evaluated by determination of minimum inhibitory concentration using the broth micro-dilution method against seven pathogenic bacterial strains (staphylococcus aureus atcc 25,923, vancomycin resistant clinical strain of enterococcus faecium, bacillus cereus ptcc 1015, escherichia coli atcc 25,922, pseudomonas aeruginosa ptcc 1430, clinical strain of klebs ... | 2016 | 26953526 |
| understanding the antimicrobial activity behind thin- and thick-rolled copper plates. | the aim of this study was to compare the antibacterial properties of the surfaces of copper plates that were rolled to a thickness of 25 and 100 μm. differences in topology of 25- and 100-μm-thick copper plates were studied using scanning electron microscopy (sem), atomic force microscopy (afm), and x-ray diffraction (xrd). antibacterial activity of the copper surfaces was tested against strains of staphylococcus aureus, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, listeria m ... | 2016 | 26860943 |
| antibacterial effects of alchornea cordifolia (schumach. and thonn.) müll. arg extracts and compounds on gastrointestinal, skin, respiratory and urinary tract pathogens. | the leaves, stems and roots of alchornea cordifolia (schumach. and thonn.) müll. arg. are used as traditional medicine in many african countries for the management of gastrointestinal, respiratory and urinary tract infections as well as for the treatment of wounds. | 2016 | 26724423 |