Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| epidemiology of respiratory syncytial virus infection among paediatric patients in hong kong: seasonality and disease impact. | in a 5-year retrospective survey of respiratory syncytial virus (rsv) infections among hospitalized children, 1340 cases were identified of which, 98.4% were children < 5 years old with a male:female ratio of 1.5: 1. most cases occurred from april to september showing a significant positive correlation with temperature and relative humidity. community-acquired infections accounted for 92.5% of the cases with a mean hospital stay of 5 days. the estimated annual incidence of rsv infection requirin ... | 1999 | 10579445 |
| the efficacy of modified-live bovine respiratory syncytial virus vaccines in experimentally infected calves. | the efficacy of modified-live (mlv) bovine respiratory syncytial virus (brsv) vaccines and the correlates of vaccine-induced immunity were investigated in calves using a virulent experimental infection. clinical disease and pulmonary pathology were significantly reduced, relative to unvaccinated controls, in calves vaccinated according to label directions with commercial multivalent mlv brsv vaccines. in vitro assays of cellular immunity were more consistent correlates of vaccine associated prot ... | 1999 | 10580205 |
| respiratory viral infections in lung transplant recipients: radiologic findings with clinical correlation. | to evaluate radiologic finding of respiratory viral infection in lung transplant recipients with clinical correlation. | 1999 | 10580947 |
| the epidemiology of respiratory syncytial virus (rsv) infections in south african children. | to review the incidence, outcomes and risk factors associated with respiratory syncytial virus (rsv) infection in south african children. | 1999 | 10582064 |
| reverse genetics of the paramyxoviridae. | 1999 | 10582105 | |
| secreted respiratory syncytial virus g glycoprotein induces interleukin-5 (il-5), il-13, and eosinophilia by an il-4-independent mechanism. | the attachment glycoprotein g of respiratory syncytial virus (rsv) is produced as both membrane-anchored and secreted forms by infected cells. immunization with secreted rsv g (gs) or formalin-inactivated alumprecipitated rsv (fi-rsv) predisposes mice to immune responses involving a th2 cell phenotype which results in more severe illness and pathology, decreased viral clearance, and increased pulmonary eosinophilia upon subsequent rsv challenge. these responses are associated with increased inte ... | 1999 | 10482601 |
| prevention of respiratory syncytial virus (rsv) infection: rsv immune globulin intravenous and palivizumab. (american academy of pediatrics)]. | 1999 | 10483596 | |
| prednisolone treatment of respiratory syncytial virus infection: a randomized controlled trial of 147 infants. | to evaluate the effect of systemic prednisolone as an adjunct to conventional treatment with beta2-agonist, respiratory support, and fluid replacement in hospitalized infants <24 months of age with respiratory syncytial virus (rsv) infection. | 1999 | 10586011 |
| respiratory syncytial virus and subsequent lower respiratory tract infections in developing countries: a new twist to an old virus. | 1999 | 10586163 | |
| respiratory illness after severe respiratory syncytial virus disease in infancy in the gambia. | to determine the frequency of later respiratory tract morbidity after respiratory syncytial virus (rsv) disease in infancy. | 1999 | 10586169 |
| comparison of vidas with direct immunofluorescence for the detection of respiratory syncytial virus in clinical specimens. | requirements for infection control measures and the decision for treatment with antiviral agents make the rapid detection of respiratory syncytial virus (rsv) essential for hospitalized, pediatric and immunocompromised patients. immunofluorescence is considered to be the most rapid and sensitive method for direct detection of rsv in clinical specimens, but several enzyme-linked immunoassays have also been commercially available. | 1999 | 10588455 |
| plasma surfactant protein-b is elevated in infants with respiratory syncytial virus-induced bronchiolitis. | respiratory syncytial virus (rsv) is the most frequent cause of bronchiolitis. however the pathophysiology of bronchiolitis is unclear. leukocytes, especially neutrophils, may play an important role in the pathogenesis of bronchiolitis. whereas we have previously shown that neutrophils augment epithelial leakage and detachment in rsv infection in vitro, it is unknown whether epithelial damage occurs in vivo in infants with rsv bronchiolitis. we hypothesized that respiratory epithelial damage occ ... | 1999 | 10590031 |
| the m2-2 protein of human respiratory syncytial virus is a regulatory factor involved in the balance between rna replication and transcription. | the m2 mrna of human respiratory syncytial virus (rsv) contains two overlapping orfs, encoding the transcription antitermination protein (m2-1) and the 90-aa m2-2 protein of unknown function. viable recombinant rsv was recovered in which expression of m2-2 was ablated, identifying it as an accessory factor dispensable for growth in vitro. virus lacking m2-2 grew less efficiently than did the wild-type parent in vitro, with titers that were reduced 1, 000-fold during the initial 2-5 days and 10-f ... | 1999 | 10500164 |
| surfactant administration in severe hypoxic respiratory failure due to respiratory syncytial virus. | 1999 | 10326183 | |
| effects of viral lower respiratory tract infection on lung function in infants with cystic fibrosis. | to determine the effect of respiratory viral infections on pulmonary function in infants with cystic fibrosis (cf) after the respiratory virus season (october through march). | 1999 | 10049966 |
| immunoprophylaxis with palivizumab, a humanized respiratory syncytial virus monoclonal antibody, for prevention of respiratory syncytial virus infection in high risk infants: a consensus opinion. | 1999 | 10093942 | |
| seroprevalence to bovine virus diarrhoea virus and other viruses of the bovine respiratory complex in venezuela (apure state). | six hundred and fifteen serum samples obtained from cows in five districts of apure state, venezuela, were tested by elisa for antibodies to bovine virus diarrhoea virus (bvdv). the same samples were also elisa-tested for antibodies to bovine herpesvirus type 1 (bhv-1) and bovine respiratory syncytial virus (brsv). additionally, the haemagglutination-inhibition (hi) test was used for detecting antibodies to parainfluenza virus type 3 (piv-3). overall, seroprevalence to bvdv was 36+/-7% (se); ser ... | 1999 | 10530426 |
| etiology of serious infections in young gambian infants. | despite improvements in infant mortality rates in many developing countries including the gambia, neonatal mortality remains high and many neonatal deaths are caused by infection. the study described in this paper was conducted to determine the bacterial and viral etiology of serious infections in gambian infants younger than 91 days old. | 1999 | 10530572 |
| bacterial and viral etiology of severe infection in children less than three months old in the highlands of papua new guinea. | determine the bacterial and viral etiology of severe infection in young papua new guinean infants as part of a multicenter study in four developing countries aimed at improving case management guidelines. | 1999 | 10530573 |
| bacterial and viral etiology of serious infections in very young filipino infants. | pneumonia, meningitis and other serious infections are leading causes of death in developing countries. as part of a multicenter study we aimed to determine the etiology of pneumonia, meningitis and other serious infections in a cohort of filipino infants ages 90 days or younger. | 1999 | 10530574 |
| etiology of pneumonia, sepsis and meningitis in infants younger than three months of age in ethiopia. | methods: within a multicenter study coordinated by who, an investigation of the etiologic agents of pneumonia, sepsis and meningitis was performed among infants younger than 3 months of age seen at the ethio-swedish children's hospital in addis ababa for a period of 2 years. of the 816 infants enrolled 405 had clinical indications for investigation. results: there were a total of 41 isolates from blood cultures from 40 infants. the study showed that the traditionally known acute respiratory infe ... | 1999 | 10530575 |
| development of local guidelines for prevention of respiratory syncytial viral infections. | 1999 | 10530578 | |
| variable morbidity of respiratory syncytial virus infection in patients with underlying lung disease: a review of the picnic rsv database. pediatric investigators collaborative network on infections in canada. | we wished to compare outcomes of respiratory syncytial virus (rsv) infection in children with bronchopulmonary dysplasia (bpd) with those with other pulmonary disorders: cystic fibrosis, recurrent aspiration pneumonitis, pulmonary malformation, neurogenic disorders interfering with pulmonary toilet, and tracheoesophageal fistula. | 1999 | 10530581 |
| macrophage inflammatory protein-1alpha and rantes are present in nasal secretions during ongoing upper respiratory tract infection. | macrophage inflammatory protein-1alpha (mip-1alpha) and rantes (regulated upon activation, normal t-cell expressed and secreted) were measured by enzyme-linked immunosorbent assay (elisa) from virus-infected respiratory cell culture supernatants and from 100 nasal wash samples obtained from patients aged 8 d to 10 yr. the results of the nasal wash samples were analyzed in relation to the etiology of the viral infection. in vitro, respiratory syncytial virus (rsv) induced the production of mip-1a ... | 1999 | 10410916 |
| near patient testing for respiratory syncytial virus in paediatric accident and emergency: prospective pilot study. | 1999 | 10426738 | |
| respiratory viruses in adult liver transplant recipients. | the contribution of respiratory viruses to respiratory disease in adult liver transplant (lt) recipients has not been studied. we performed a prospective audit to document the incidence of respiratory syncytial viruses ([rsvs], parainfluenza virus, influenza virus, and adenovirus) after lt, and to determine their contribution to respiratory disease in this setting. | 1999 | 10532538 |
| impact-rsv study group report. | 1999 | 10532864 | |
| impact-rsv study group report. | 1999 | 10532865 | |
| impact-rsv study group report. | 1999 | 10532866 | |
| near patient test for respiratory syncytial virus identifies positive cases | 1999 | 10426778 | |
| bronchiolitis-associated hospitalizations among us children, 1980-1996. | respiratory syncytial virus (rsv) causes more lower respiratory tract infections, often manifested as bronchiolitis, among young children than any other pathogen. few national estimates exist of the hospitalizations attributable to rsv, and recent advances in prophylaxis warrant an update of these estimates. | 1999 | 10535434 |
| jama patient page: respiratory problems. | 1999 | 10535442 | |
| exposure to cigarette smoke, a major risk factor for sudden infant death syndrome: effects of cigarette smoke on inflammatory responses to viral infection and bacterial toxins. | exposure to cigarette smoke is a major risk factor for sudden infant death syndrome and also for respiratory infections in children. it has been suggested that toxigenic bacteria colonizing the respiratory tract might play a role in some cases of sudden infant death syndrome and nicotine has been demonstrated to enhance the lethality of bacterial toxins in a model system. pyrogenic toxins of staphylococcus aureus have been identified in tissues of infants who died of sudden infant death syndrome ... | 1999 | 10443503 |
| expression and characterisation of the ovine respiratory syncytial virus (orsv) g protein for use as a diagnostic reagent. | respiratory syncytial virus (rsv) causes severe lower respiratory tract infection in children and calves. antibodies to ovine rsv (orsv) are common in sheep, but the clinical disease is not well defined. there is no report of orsv infection in australian sheep although respiratory distress syndrome has been described. this discrepancy may be due to the lack of a suitable diagnostic test. in this report, we have characterised the orsv g protein in an attempt to study its relatedness to human and ... | 1999 | 10543382 |
| open reading frame in rotavirus mrna specifically promotes synthesis of double-stranded rna: template size also affects replication efficiency. | the 11 rotavirus mrnas are capped, but not polyadenylated, have a high au content, and serve as templates for the synthesis of double-stranded (ds)rna. earlier studies using a cell-free replication system showed that the 5'- and 3'-untranslated regions (utrs) of the mrnas have cis-acting signals that promote minus-strand synthesis. to identify additional factors that affect rna replication, chimeric rnas were made that consisted of portions of the gene 8 mrna of sa11 rotavirus and of the gene fo ... | 1999 | 10544142 |
| mutations of respiratory syncytial virus attachment glycoprotein g associated with resistance to neutralization by primate polyclonal antibodies. | the respiratory syncytial (rs) virus attachment glycoprotein g is a type ii transmembrane glycoprotein and an important target of the host immune response. antigenic variability of the g protein is postulated to contribute to the ability of the virus to evade established immune responses. a glycoprotein g monospecific polyclonal antiserum from a nonhuman primate was used to select for an antibody resistant rs virus. the mutant virus was resistant to neutralization by the selecting antiserum and ... | 1999 | 10544149 |
| protective efficacy against respiratory syncytial virus following murine neonatal immunization with bbg2na vaccine: influence of adjuvants and maternal antibodies. | alum-adsorbed bbg2na, a recombinant vaccine derived in part from the respiratory syncytial virus (rsv) subgroup a g protein, induced moderate antibody titers after 1 immunization in 1-week-old mice but conferred complete lung protection upon rsv challenge. the anti-bbg2na igg1-igg2a neonatal isotype profile was suggestive of dominant th2 responses compared with those in adults. formulation of bbg2na with a th1-driving adjuvant efficiently shifted neonatal responses toward a more balanced and adu ... | 1999 | 10228051 |
| advances in prevention of respiratory syncytial virus infections. | 1999 | 10547241 | |
| [detection of respiratory syncytial virus using immunohistochemistry. report of 53 cases of sudden infant death]. | we analyzed 53 cases of sudden infant death to detect immunohistochemical expression of respiratory syncytial virus on pulmonary sections. the virus was identified in 7 cases. the immunohistochemical staining was intracytoplasmic and mainly observed in bronchioles. among these 7 cases, 6 showed severe pulmonary lesions which were assumed to be accountable for decrease. the inflammatory lesions related to respiratory syncytial virus were diffuse, located with the same intensity to either bronchi, ... | 1999 | 10349473 |
| association of radiologically ascertained pneumonia before age 3 yr with asthmalike symptoms and pulmonary function during childhood: a prospective study. | epidemiologic evidence suggests an association between reports of pneumonia in early life and the subsequent development of diminished lung function. however, no studies are available in which the diagnosis of pneumonia was based on radiologic evidence. lower respiratory illnesses with or without a radiologically confirmed diagnosis of pneumonia were assessed in a study of 888 children enrolled at birth. pulmonary function tests, markers of atopy, asthma diagnosis, and prevalence of respiratory ... | 1999 | 10351936 |
| crystal structure of two cd46 domains reveals an extended measles virus-binding surface. | measles virus is a paramyxovirus which, like other members of the family such as respiratory syncytial virus, is a major cause of morbidity and mortality worldwide. the cell surface receptor for measles virus in humans is cd46, a complement cofactor. we report here the crystal structure at 3.1 a resolution of the measles virus-binding fragment of cd46. the structure reveals the architecture and spatial arrangement of two glycosylated short consensus repeats with a pronounced interdomain bend and ... | 1999 | 10357804 |
| rescue of a bovine respiratory syncytial virus genomic rna analog by bovine, human and ovine respiratory syncytial viruses confirms the "functional integrity" and "cross-recognition" of brsv cis-acting elements by hrsv and orsv. | the nucleotide sequences of the 3' leader and 5' trailer regions were determined for genomic rna of bovine respiratory syncytial virus (brsv) strain a-51908. the leader and trailer sequences are '45' and '161' nucleotides in length, respectively. the functionality of brsv leader and trailer sequences and their recognition by hrsv and ovine respiratory syncytial virus (orsv) proteins were examined with a in vitro transcribed brsv genomic rna analog carrying the bacterial chloramphenicol acetyl tr ... | 1999 | 10550670 |
| transfer of the enhancing effect of respiratory syncytial virus infection on subsequent allergic airway sensitization by t lymphocytes. | in mice, respiratory syncytial virus (rsv) infection enhances allergic airway sensitization, resulting in lung eosinophilia and in airway hyperresponsiveness (ahr). the mechanisms by which rsv contributes to development of asthma and its effects on allergic airway sensitization in mice are not known. we tested whether these consequences of rsv infection can be adoptively transferred by t cells and whether depletion of t cell subsets prevents the effects of rsv infection on subsequent airway sens ... | 1999 | 10553105 |
| antigenic and genetic characterization of twenty-six strains of human respiratory syncytial virus (subgroup a) isolated during three consecutive outbreaks in havana city, cuba. | twenty-six human respiratory syncytial virus strains (subgroup a) isolated from three outbreaks in havana city during the period 1994/95, 1995/96 and 1996/97 were analyzed to determine their antigenic and genetic relationships. analyses were performed by monoclonal antibodies and restriction mapping (n gene) following amplification of the select region of the virus genome by polymerase chain reaction. all isolated strains were classified as subgroup a by monoclonal antibodies and they showed a r ... | 1999 | 10446003 |
| a recombinant trimeric surfactant protein d carbohydrate recognition domain inhibits respiratory syncytial virus infection in vitro and in vivo. | the pulmonary collectin, lung surfactant protein d (sp-d), plays a role in host defense mediated by the interaction of surface carbohydrates of inhaled pathogens with the lectin domains of sp-d. respiratory syncytial virus (rsv), the most important viral pathogen of neonates and infants, encodes a highly glycosylated attachment protein, g. binding studies were performed with g protein from rsv (human, a2 strain) and both native and recombinant human sp-d. the effect of recombinant trimeric sp-d ... | 1999 | 10556802 |
| surfactant protein a binds to the fusion glycoprotein of respiratory syncytial virus and neutralizes virion infectivity. | collectins are a family of calcium-dependent collagenous lectins that appear to be important in innate host defense. we investigated the ability of three human collectins, namely, lung surfactant proteins a (sp-a) and d (sp-d) and the serum mannose-binding protein (mbp), to bind to the surface glycoproteins of respiratory syncytial virus (rsv). sp-a was shown to bind to the f (fusion) glycoprotein but not to the viral g (attachment) glycoprotein, and binding was completely abrogated in the prese ... | 1999 | 10558959 |
| replacement of the f and g proteins of respiratory syncytial virus (rsv) subgroup a with those of subgroup b generates chimeric live attenuated rsv subgroup b vaccine candidates. | human respiratory syncytial virus (rsv) exists as two antigenic subgroups, a and b, both of which should be represented in a vaccine. the f and g glycoproteins are the major neutralization and protective antigens, and the g protein in particular is highly divergent between the subgroups. the existing system for reverse genetics is based on the a2 strain of rsv subgroup a, and most efforts to develop a live attenuated rsv vaccine have focused on strain a2 or other subgroup a viruses. in the prese ... | 1999 | 10559287 |
| priming with a secreted form of the fusion protein of respiratory syncytial virus (rsv) promotes interleukin-4 (il-4) and il-5 production but not pulmonary eosinophilia following rsv challenge. | the attachment (g) protein of respiratory syncytial virus (rsv) is synthesized as two mature forms: a membrane-anchored form and a smaller secreted form. balb/c mice scarified with vaccinia virus (vv) expressing the secreted form develop a greater pulmonary eosinophilic influx following rsv challenge than do mice scarified with vv expressing the membrane-anchored form. to determine if a soluble form of an rsv protein was sufficient to induce eosinophilia following rsv challenge, a cdna that enco ... | 1999 | 10559323 |
| pulmonary failure after norwood procedure: indication for extracorporeal membrane oxygenation? a case report. | today some authors consider univentricular repair a contraindication for postoperative cardiac extracorporeal membrane oxygenation (ecmo). the question is whether or not ecmo is indicated as pulmonary support in case of an overwhelming pulmonary infection during the postoperative course after a norwood procedure. during the prolonged weaning period after a norwood procedure using a 4 mm aortopulmonary shunt, proven respiratory syncytial virus (rsv) bronchiolitis occurred at the time of expected ... | 1999 | 10564313 |
| inhaled antimicrobial therapy. | although antimicrobial therapy has been administered through the inhaled route for decades, it has always been controversial. there are relatively few accepted indications for this mode of administration. well-controlled studies of aerosolized antibiotics in cystic fibrosis demonstrate that tobramycin on a cyclical basis may reduce sputum volume, bacterial counts, and improve pulmonary function. preliminary data indicate that inhaled antibiotic therapy of ventilator-associated tracheobronchitis ... | 1999 | 10565884 |
| prophylaxis for respiratory syncytial virus bronchiolitis. | 1999 | 10622321 | |
| respiratory syncytial virus and wheeze. | 1999 | 10622322 | |
| intranasal ifn-gamma gene transfer protects balb/c mice against respiratory syncytial virus infection. | respiratory syncytial virus (rsv) is a major respiratory pathogen in infants, young children and the elderly and causes severe bronchiolitis and asthma. in an effort to develop a preventive ifn-gamma therapy against rsv infection, an intranasal gene transfer strategy was utilized. intranasal administration of a plasmid expressing the ifn-gamma cdna (pifn-gamma) resulted in the expression of ifn-gamma in murine lungs and decreased rsv replication. the mice administered with pifn-gamma and then in ... | 1999 | 10519947 |
| high-frequency oscillatory ventilation in rsv bronchiolitis patients. | 1999 | 10464828 | |
| the outcome of 26 patients with respiratory syncytial virus infection following allogeneic stem cell transplantation. | respiratory syncytial virus (rsv) is known to cause acute lung injury in the immunocompromised host, especially recipients of bone marrow allografts. specific prognostic factors for the development of severe life-threatening disease remain to be identified as does the optimum treatment of established disease. over a 5-year period the incidence and outcome of rsv in bmt recipients was analysed retrospectively. prognostic factors assessed included type of transplant, engraftment status at the time ... | 1999 | 10627641 |
| alteration of leukocyte populations in calves concurrently infected with bovine respiratory syncytial virus and bovine viral diarrhea virus. | bovine viral diarrhea virus (bvdv) infection altered leukocyte populations in calves that were reflected by depression of t, bocd4+, and bocd8+ lymphocytes in the thymus and depression of b lymphocytes in peyer's patches (pp). the present study was based on mononuclear leukocyte preparations from eighteen 9- to 12-month-old crossbred calves that were each exposed to either bovine respiratory syncytial virus (brsv), bvdv, or brsv and bvdv concurrently, or served as mock-infected controls. periphe ... | 1999 | 10630791 |
| update: respiratory syncytial virus activity--united states, 1998-1999 season. | respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract disease in infants and young children worldwide. in temperate climates, rsv infections occur primarily during annual outbreaks, which peak during winter months . in the united states, rsv activity is monitored by the national respiratory and enteric virus surveillance system (nrevss), a voluntary, laboratory-based system. this report summarizes trends in rsv activity reported to nrevss during july 1998 june 199 ... | 1999 | 10632299 |
| [respiratory syncytial virus]. | 1999 | 10635845 | |
| airway epithelial cell-induced activation of monocytes and eosinophils in respiratory syncytial viral infection. | the early inflammatory events in respiratory syncytial viral (rsv) infection are likely to be crucial in the development of clinical disease, which is characterized by bronchiolitis with mononuclear cell inflammation, some eosinophil involvement and airway hyperreactivity. since rsv replication is restricted to airway epithelial cells, our working hypothesis is that inflammatory cell recruitment by the infected cells will set the stage for late immunopathology. we have identified the selective i ... | 1999 | 10532283 |
| mxa protein in capillary blood of children with viral infections. | capillary blood of febrile children was lysed by using a lysis buffer containing ascorbic acid. mxa quantitation was performed by an immunochemiluminescent assay. the mxa values were significantly higher in capillary blood of infants with viral infections due to adenovirus (n = 5), rotavirus (n = 15), or respiratory syncytial virus (n = 28), than in capillary whole blood from infants with bacterial infections (n = 6) and healthy control patients (n = 20). a strong correlation was found between t ... | 1999 | 10534740 |
| tumor necrosis factor-alpha and interleukin-6 profiles in children with pneumonia. | pneumonia is a common cause of hospitalization and is associated with high morbidity in children. tumor necrosis factor-alpha (tnf-alpha) and interleukin-6 (il-6) are primary mediators of inflammation, and have been implicated in a large number of infectious and non-infectious inflammatory diseases. the serum concentrations of tnf-alpha and il-6 were measured by enzyme-linked immunosorbent assay (elisa) in 27 patients with bacterial pneumonia (n = 12) or respiratory syncytial virus (rsv) pneumon ... | 1999 | 10650487 |
| combining dna and protein vaccines for early life immunization against respiratory syncytial virus in mice. | early life responses to respiratory syncytial virus (rsv)-f dna and rsv-f protein immunization were studied in murine models of neonatal immunization. rsv-f dna induced similar antibody (ab) responses, antigen-specific ifn-gamma production and cytotoxic t lymphocyte (ctl) responses in 1-week-old and adult balb / c mice. in contrast, rsv-f protein induced much higher il-5 responses in early life. both vaccines elicited ab and ctl responses in spite of maternal ab, but with distinctive kinetics. s ... | 1999 | 10540351 |
| the pitfalls of using managed care databases in cost-effectiveness research. | 1999 | 10545557 | |
| mononuclear leukocytes preferentially bind via cd44 to hyaluronan on human intestinal mucosal smooth muscle cells after virus infection or treatment with poly(i.c). | pathological changes in inflammatory bowel disease include an increase in intestinal mucosal mononuclear leukocytes and hyperplasia of the muscularis mucosae smooth muscle cells (m-smcs). because virus infections have correlated with disease flare, we tested the response of cultured m-smcs to respiratory syncytial virus, measles virus, and the viral analogue, poly(i.c). adhesion of u937 cells and peripheral blood mononuclear cells was used to measure the leukocyte-interactive potential of m-smcs ... | 1999 | 10521464 |
| [development of new approaches to standartization of enzyme immunoassay test systems for detection of antibodies to respiratory syncytial virus using electron microscopy and monoclonal antibodies]. | respiratory syncytial virus (rsv), strain long, was purified through 20-60% sucrose gradient. the virions from different sucrose density zones were tested by elisa for reactivity with monoclonal antibodies (mab) to f- (mab 9c5) and n- (mab 8b10) proteins of rsv. comparative study of the same patterns of rsv by electron microscopy after negative staining showed a close relationship between the virion morphology and mab binding in elisa. mab 9c5 were highly reactive with the surface domains of bot ... | 1999 | 10665066 |
| community study using a polymerase chain reaction panel to determine the prevalence of common respiratory viruses in asthmatic and nonasthmatic children. | we developed a sensitive polymerase chain reaction (pcr) panel, suitable for the detection of seven common respiratory viruses, to study the prevalence of viruses in nasal swabs obtained from clinically stable asthmatic children (n = 21), non-physician diagnosed asthmatic children with exercise-induced bronchoconstriction (eib) (n = 16), and nonasthmatic, non-eib controls (n = 33). the pcr panel detected viruses in 43/70 (61.4%) specimens but there were no significant differences in prevalence o ... | 1999 | 10524544 |
| carrier properties of a protein derived from outer membrane protein a of klebsiella pneumoniae. | we have recently cloned a new protein, recombinant p40 (rp40). when tested in vivo after conjugation to a b-cell epitope, rp40 induces an important antibody response without the need for adjuvant. to characterize its potency, this carrier protein was coupled to a peptide derived from respiratory syncytial virus attachment g protein (g1'). after immunization of mice with the rp40-g1' conjugate, strong antipeptide antibodies were detected, whereas peptide alone was not immunogenic. to emphasize th ... | 1999 | 10531198 |
| palivizumab for respiratory syncytial virus prophylaxis. | palivizumab, a humanized monoclonal antibody, has been approved by the fda to prevent severe lower respiratory tract infections caused by rsv in high-risk patients. prophylaxis of rsv infections with palivizumab requires monthly injections (15 mg/kg) during the rsv season. in the impact-rsv study, hospitalizations resulting from rsv decreased by 55% in the palivizumab treatment group. palivizumab has also been shown to decrease the number of days with moderate or severe rsv infection, with an in ... | 1999 | 10690084 |
| respiratory syncytial virus: understanding the threat to communities. | 1999 | 10692709 | |
| pathological and microbiological studies on pneumonic lungs from danish calves. | during 1 year, the association between microbiological and pathological findings in 72 lungs from calves submitted to the danish veterinary laboratory for diagnostic purposes was studied. all cases were evaluated pathologically and bacteriologically, whereas only 68 cases were examined for the presence of bovine respiratory syncytial virus (brsv), parainfluenza-3 virus (pi-3 virus) and bovine coronavirus, 62 cases for bovine viral diarrhoea virus (bvd), 45 cases for bovine adenovirus and 51 case ... | 1999 | 10676147 |
| detection of igm responses to bovine respiratory syncytial virus by indirect elisa following experimental infection and reinfection of calves: abolition of false positive and false negative results by pre-treatment of sera with protein-g agarose. | the igm responses in three panels of sera generated by infection and reinfection of calves with bovine respiratory syncytial virus (brsv) were measured by indirect elisa (i-elisa). the effect of depleting serum igg by pre-treatment with protein g agarose (pga) was evaluated. following primary infection a weak igm response was detected in the untreated sera of 3 out of 4 calves with maternally derived antibody (mda). both the magnitude and duration of the specific igm responses in these calves we ... | 1999 | 10522785 |
| inhibitory effect of ferulic acid and isoferulic acid on the production of macrophage inflammatory protein-2 in response to respiratory syncytial virus infection in raw264.7 cells. | we investigated the effect of ferulic acid (fa) and isoferulic acid (ifa), which are the main active components of the rhizoma of cimicifuga heracleifolia (ch), an anti-inflammatory drug used frequently in japanese traditional medicine, on the production of macrophage inflammatory protein-2 (mir-2) in a murine macrophage cell line, raw264.7, in response to respiratory syncytial virus (rsv) infection. following the exposure of cells to rsv for 20h, the mip-2 level in condition medium was increase ... | 1999 | 10704056 |
| respiratory syncytial virus: current status and hope for the future. | 1999 | 10710796 | |
| [practice guideline by the german society for pediatric infectious diseases with respect to prevention of rsv infections through immunoglobulin administration]. | 1999 | 10592932 | |
| palivizumab: an overview. | respiratory syncytial virus (rsv) affects almost all children in their first 2 years of life and can cause severe or even life-threatening disease in some at-risk infants. treatment is limited and there is currently no safe or effective vaccine. however, a new monoclonal antibody, palivizumab, reduces rsv hospitalization by 55% in at-risk groups if given prophylactically throughout the rsv season. | 1999 | 10707171 |
| respiratory syncytial virus: an underestimated cause of respiratory infection, with prospects for a vaccine. | respiratory syncytial virus (rsv) infects most people by the time they are 2 years old and reinfects throughout life. rsv is best recognised for causing bronchiolitis in infants--it is one of the most important respiratory pathogens in childhood in industrialised countries. the clinical manifestations of rsv infection in adults and elderly people, from upper respiratory tract infection to pneumonia, are less well known. part of the burden of winter mortality in elderly people is attributable to ... | 1999 | 10598379 |
| eosinophils, ribonucleases and host defense: solving the puzzle. | the eosinophil ribonucleases eosinophil-derived neurotoxin (edn/rnase 2) and eosinophil cationic protein (ecp/rnase 3) are among the major secretory effector proteins of human eosinophilic leukocytes, cells whose role in host defense remains controversial and poorly understood. we have recently described the unusual manner in which this ribonuclease lineage has evolved, with extraordinary diversification observed in primate as well as in rodent edns and ecps. the results of our evolutionary stud ... | 1999 | 10741866 |
| prevention and treatment of respiratory syncytial virus infections. | 1999 | 10549378 | |
| childhood viral infection and the pathogenesis of asthma and chronic obstructive lung disease. | many epidemiologic studies have implicated childhood respiratory infections as an independent risk factor for the subsequent development of persistent asthma and chronic obstructive pulmonary disease (copd). the majority of these childhood infections are viral in origin, and great strides are being made in understanding their pathogenesis at the molecular level. some viruses, such as respiratory syncytial virus-a common cause of childhood bronchiolitis-stimulate the helper t cell type 2 (th2) pa ... | 1999 | 10556165 |
| extrapontine myelinolysis with involvement of the hippocampus in three children with severe hypernatremia. | central pontine myelinolysis is a disorder of unknown etiology linked to overly aggressive correction of hyponatremia. in addition to the typical location of demyelination with preservation of neurons and axon cylinders in the basis pontis, similar lesions have been described in extrapontine locations. central pontine myelinolysis and extrapontine myelinolysis usually occur together, and are identified at autopsy rather than in life because symptoms of extrapontine myelinolysis are often masked ... | 1999 | 10573464 |
| pulmonary lesions in primary respiratory syncytial virus infection, reinfection, and vaccine-enhanced disease in the cotton rat (sigmodon hispidus). | infection of the cotton rat lung with a human strain of respiratory syncytial virus results in substantial virus replication and is associated with mild-to-moderate peribronchiolitis, perivasculitis, and bronchitis. reinfection after 49 days did not result in detectable virus replication, but surprisingly, was associated with an earlier appearance and accentuation of the three types of lesions seen in cotton rats undergoing primary infection. animals primed with formalin-inactivated virus and ch ... | 1999 | 10576209 |
| 2-5a-antisense chimeras: inhibitors of respiratory syncytial virus infection. | a new approach to the chemical control of respiratory syncytial virus infection is reviewed in the context of the biology of the virus and previous treatment approaches. conjugation of the interferon action mediator, 2',5'-oligoadenylate (2-5a), to antisense agents provides a strategy for the selective ablation of rna. this application of this technology to respiratory syncytial virus has provided a potent selective inhibitor of virus replication. | 1999 | 11713795 |
| [mite-induced interleukin-5 and interleukin-4 expressions in peripheral blood mononuclear cells from asthmatic children with respiratory syncytial virus infection]. | to investigate the effects of respiratory syncytial virus(rsv) infection on mite-induced interleukin-5 and interleukin-4 expressions in cultures of pbmc from asthmatic children and their correlation with asthmatic symptoms. | 1999 | 11775916 |
| the associations of viral and mycoplasmal antibody titers with respiratory disease and weight gain in feedlot calves. | blood samples from 32 groups of calves (n = 700) were taken on arrival and after 28-35 days at the feedlot. eleven groups were housed in feedlots in ontario, and 21 groups in feedlots in alberta. serum antibody titers to bovine viral diarrhea virus (bvdv), bovine respiratory syncytial virus (brsv), parainfluenza virus type 3 (piv-3), infectious bovine rhinotracheitis virus (ibrv), mycoplasma dispar and m. bovis, plus data on bovine corona virus (bcv) from a previous study were investigated for t ... | 1999 | 12001336 |
| [cloning and sequencing of cdna from g protein gene of subgroup b respiratory syncytial virus strain isolated in china]. | the nucleotide sequence of the g protein gene of respiratory syncytial virus (rsv) cc169 strain isolated from china that has been identified as subgroup b with monoclonal antibodies, was determined from cdna that had been amplified by rt-pcr and cloned into ptz18r plasmid vector. the homology of nucleotide was 94% as compared with g protein cdna of a rsv prototype strain (ch18537). deduced amino acid identity of g protein was 89.4%. the amino acid changes were only in the extracellular part of t ... | 1999 | 12555397 |
| [the detection of virus antigen in the lower respiratory tract of the patients with lung cancer]. | to find out about the viral infection situation of lower respiratory tract of the patients with lung cancer. | 1999 | 12759981 |
| [molecular epidemiology of respiratory syncytial virus infection in infants]. | to search for the molecular epidemiology of respiratory syncytial virus (rsv) infection in infants. | 1999 | 12759984 |
| synagis: an anti-rsv monoclonal antibody. | palivizumab is a new anti-rsv monoclonal antibody product indicated for the prevention of serious lower respiratory tract disease caused by rsv in pediatric patients at high risk of developing rsv disease ("synagis," 1998). palivizumab was evaluated in a single, large clinical trial (impact) (connor et al., 1998). in this trial, rsv hospital admissions were reduced by 55% in children 24 months of age with bpd or in infants born prematurely (35 weeks gestation). additionally, the severity of illn ... | 1999 | 12024401 |
| asthma in inner cities. | while the management of asthma has improved over the past two decades, the incidence of asthma in the inner city has not. the inner city, comprising a disproportionate number of people who live close to or below the poverty line, shows increased rates of morbidity and mortality from asthma. african americans and hispanic americans are two to six times more likely to die from asthma than their white counterparts. when federally funded programs have targeted reducing morbidity and mortality in chi ... | 1999 | 12653387 |
| diagnosis of enzootic pneumonia in danish cattle: reverse transcription-polymerase chain reaction assay for detection of bovine respiratory syncytial virus in naturally and experimentally infected cattle. | a reverse transcription-polymerase chain reaction (rt-pcr) assay was developed for detection of bovine respiratory syncytial virus (brsv) in lung tissue of naturally and experimentally infected cattle. primers were selected from the gene coding the f fusion protein, which is relatively conserved among brsv isolates. the rt-pcr assay was highly specific, it yielded positive reactions only when performed on brsv-infected cell cultures or tissues. the detection limit of the rt-pcr assay was assesse ... | 1999 | 12968754 |
| [viral infections in hospitalized children affected by acute lower respiratory tract disease] | objective: to evaluate the frequency of the main respiratory viruses in hospitalized children affected by acute lower respiratory tract disease at a university hospital. methods: this is a prospective trial that included two cohorts of hospitalized children in the period from april to july 1996. the groups were selected according to the presence of lower respiratory tract disease on admission: group a- with acute disease (history of less than 7 days) and b- without present or recent respiratory ... | 1999 | 14685511 |
| neutralization of hiv-1 infection of human peripheral blood mononuclear cells (pbmc) : antibody dilution method. | antibodies elicited by passive or active immunization protect against numerous virus diseases. this in vivo protective immunity is often associated with in vitro detection of neutralizing antibodies (nab) (e.g., polio, measles, influenza, respiratory syncytial virus, yellow fever, dengue, rabies, varicella, hepatitis a, hepatitis b) (1-7). since human protection from hiv-1-associated disease has not yet been achieved, the role of nab in protective immunity is not defined. nonetheless, based on e ... | 1999 | 21380682 |
| a microcarrier-based cell culture process for the production of a bovine respiratory syncytial virus vaccine. | veterinary viral vaccines generally comprise either attenuated or chemically inactivated viruses which have been propagated on mammalian cell substrates or specific pathogen free (spf) eggs. new generation vaccines include chemically inactivated virally-infected whole cell vaccines. the nm57 cell line is a bovine nasal turbinate persistently infected (non-lytic infection) with a strain of the respiratory syncytial virus (rsv). the potential of microcarrier technology for the cultivation in biore ... | 1999 | 22359062 |
| palivizumab and respiratory syncytial virus immune globulin intravenous for the prophylaxis of respiratory syncytial virus infection in high risk infants. | 1999 | 20212962 | |
| analysis of antibiotic use and misuse in children hospitalized with rsv infection. | to determine the extent of, rationale for and acquisition cost of antibiotic use in a cohort of children with lower respiratory tract infection (lri) secondary to the respiratory syncytial virus (rsv). | 1999 | 20212965 |
| response to modified live and killed multivalent viral vaccine in regularly vaccinated, fresh dairy cows. | vaccination programs for viral pathogens in the dairy industry span the full spectrum of possibilities even though few of these have been evaluated in field situations. one such program is the vaccination of fresh cows 30 to 60 days postpartum with modified live viral (mlv) vaccines. the purpose of this study was to evaluate the antibody response to booster vaccinations during this period. the impact of vaccinations on milk production and reproductive performance was also examined. the response ... | 2000 | 19757565 |
| respiratory syncytial virus infection: an emerging or unappreciated infection? | respiratory syncytial virus (rsv) is a ubiquitous cause of respiratory infection with a worldwide distribution and seasonal occurrence. natural immunity does not normally follow infection, and reinfection is the rule. reinfection may even occur within a single season. although its role in serious lower respiratory tract infection (lrti) in infants is well recognized, the importance of rsv in disease of older children, adults and, especially in the elderly, is poorly appreciated. in large surveil ... | 2000 | 16088741 |
| prevention of bronchiolitis. | this review evaluates the current situation and long-term prospects for containment of human respiratory syncytial virus (hrsv) infection and bronchiolitis in infancy. the biology and immunopathology of hrsv infection are complex. initial attempts to control hrsv infection using a conventional formalin-inactivated vaccine had the unexpected outcome that the disease was potentiated in some vaccinees experiencing natural hrsv infection at a later date. much effort has been devoted to defining the ... | 2000 | 12531084 |
| surveillance for respiratory illness in long-term care settings: detection of illness using a prospective research technique. | the overall frequency and severity of viral respiratory infections affecting residents of long-term care facilities (ltcfs) is not well described. this is due primarily to the cumbersome and expensive techniques required for adequate surveillance of respiratory illnesses and the associated costs and availability of a laboratory capable of the relevant and timely report of diagnostic tests. here we describe our technique for surveillance of respiratory illness in the ltcf. elements of it may serv ... | 2000 | 12818025 |
| myocardial injury in children with respiratory syncytial virus infection. | respiratory syncytial virus (rsv) infection is associated with a number of extrapulmonary manifestations, including a sepsis-like syndrome characterized by any combination of hypothermia, fever, apnea, hypovolemia, and myocardial dysfunction. we hypothesized that rsv can have a direct injurious effect on the myocardium of infants and children that can be detected by the presence of cardiac troponin i (ctni), a highly sensitive and specific marker of myocardial injury, in the blood of patients in ... | 2000 | 12813266 |