Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| nucleotide sequence of the srrna gene of entamoeba gingivalis: applications for construction of a species-specific dna probe and phylogenetic analysis. | the small subunit ribosomal rna (srrna) gene of entamoeba gingivalis was amplified by pcr and the product of 1.9-kbp sequence was cloned into a plasmid vector puc18. four clones were isolated and sequenced. the insert dnas were 1918- to 1921-bp long and a+t rich (65.5%). the four srrna sequences of e. gingivalis were found to be aligned with those of nine related protozoans while searching for e. gingivalis-specific sequences. a sequence of 28 oligonucleotides was chosen, chemically synthesized, ... | 1995 | 7603363 |
| direct evidence for secondary loss of mitochondria in entamoeba histolytica. | archezoan protists are though to represent lineages that diverged from other eukaryotes before acquisition of the mitochondrion and other organelles. the parasite entamoeba histolytica was originally included in this group. ribosomal rna based phylogenies, however, place e. histolytica on a comparatively recent branch of the eukaryotic tree, implying that its ancestors had these structures. in this study, direct evidence for secondary loss of mitochondrial function was obtained by isolating two ... | 1995 | 7604025 |
| [amebic appendicitis with subsequent perforated pancolitis]. | amoebic appendicitis is very rare, occurring in about 0.5 to 1% of acute appendicitis in tropical countries. the most severe complication is transmural amoebic colitis with perforation, described in 1.6 to 3.2% of cases. the mortality of such cases can be very high (up to 80%). we present a swiss patient with amoebic appendicitis followed by severe perforated colitis; a total colectomy was necessary and the patient survived. because of the good results of amoebicidal therapy and because of the s ... | 1995 | 7607020 |
| isolation and characterization of species-specific dna probes from taenia solium and taenia saginata and their use in an egg detection assay. | cysticercosis results from ingestion of the eggs of the tapeworm taenia solium. reduction of the incidence of human and swine cysticercosis requires identification and treatment of individuals who carry the adult tapeworm. t. solium and taenia saginata eggs cannot be differentiated on the basis of morphology; thus, in order to improve existing methods for the diagnosis of taeniasis, we have developed highly sensitive, species-specific dna probes which differentiate t. solium and t. saginata. rec ... | 1995 | 7615742 |
| characterization of glycogen and amino acid pool of entamoeba histolytica by 13c-nmr spectroscopy. | trophozoite extracts of axenic entamoeba histolytica were investigated by natural-abundance 13c-nmr spectroscopy. the extracts were found to contain a high level of glycogen (30 mm glucose equivalents), which had a compact structure as suggested by alpha (1-->6) branch points every 5-6 glucose residues. as other major metabolites, we identified putrescine (9.5 mm) and the following free amino acids: tyrosine and phenylalanine (1 mm), glycine, lysine and methionine (2 mm), isoleucine (5 mm), prol ... | 1995 | 7620458 |
| an unusual amebic localization on the right hip. | we report the history of a 43-year-old man with a histopathologically confirmed invasive amebic abscess in his right hip. ct scan of the liver was normal. the amebic indirect hemagglutination (iha) test was positive with a titer of 1/1,024. the patient developed acute renal failure and died within 48 h of admission with multiple organ failure due to sepsis. | 1995 | 7622262 |
| development of amebicidal cell-mediated immunity in gerbils (meriones unguiculatus) immunized with the galactose-inhibitable adherence lectin of entamoeba histolytica. | the galactose-inhibitable adherence lectin of entamoeba histolytica is a protective antigen in the gerbil model of amebic liver abscess. to further understand the mechanisms of vaccine efficacy, we studied the cell-mediated immune response to the lectin in gerbils. splenocytes harvested from immunized gerbils demonstrated in vitro proliferation and production of interleukin-2 and gamma-interferon in response to purified adherence lectin (p < 0.05 for each compared to control splenocytes). spleno ... | 1995 | 7623198 |
| identification of entamoeba histolytica intracellular phospholipase a and lysophospholipase l1 activities. | entamoeba histolytica phospholipase a and lysophospholipase activities from a vesicular subcellular fraction (p30) were analyzed. the products, obtained using specific substrates labeled with 14c or 3h, indicated the presence of phospholipase a1 and a2 as well as lysophospholipase l1 activities. the enzymes detected could participate in phospholipid metabolism and the alkaline phospholipase a2 may contribute to e. histolytica cytopathogenicity. | 1995 | 7624291 |
| how many stool examinations are necessary to detect pathogenic intestinal protozoa? | recent reports have suggested that examination of one stool specimen is sufficient to detect protozoa in symptomatic patients. to verify this hypothesis, we examined specimens submitted to the regional laboratory of a large, prepaid health plan that provides primary care to a membership representative of the general population in northern california. we examined the number and results of stool specimen examinations in the two-month period before detection of entamoeba histolytica in 125 infected ... | 1995 | 7625530 |
| pattern of infection of intestinal parasites in sagbama community of the niger delta, nigeria. | a parasitology survey was conducted in sagbama community of the niger delta to determine the pattern of infection of intestinal parasites of man. 280 faecal samples were collected from randomly selected individuals and examined microscopically for ova, larvae and cysts of intestinal parasites. the overall infection rate was 33.6%. the frequency of the parasites encountered was as follows: ascaris lumbricoides (18.2%). entamoeba histolytica (6.4%). hookworm (5.4%). trichuris trichiura (3.5%), str ... | 1995 | 7626531 |
| multidrug resistance and p-glycoproteins in parasitic protozoa. | drug resistance has emerged as a devasting impediment to the treatment and control of diseases of parasitic origin. the underlying mechanisms that contribute to this drug resistance in field isolates, however, are poorly understood. members of the p-glycoprotein gene (pgp) family have been identified, cloned, and sequenced in plasmodia, leishmania, and entamoeba, and variations in pgp copy number and/or expression have been implicated as a basis for drug resistance in each of these genera. the s ... | 1995 | 7629055 |
| health status of pediatric refugees in buffalo, ny. | to characterize the health status of recent pediatric refugees. | 1995 | 7633543 |
| [gastrointestinal and urinary parasitic infection in children at a regional hospital center in togo: some epidemiological aspects]. | to determine the impact of parasitic infection of the digestive and urinary tract in children living in a rural area of togo, a retrospective study was conducted in a pediatric department of kara, togo. results revealed that 35% of the 1610 children between the ages of 0 and 16 years had positive tests for parasites in stools or urine and that 117 had more than one parasite. trichomonas intestinalis, entamoeba histolytica, schistosoma mansoni and necator americanus accounted for 86.5% of the par ... | 1995 | 7637613 |
| identification of an entamoeba histolytica gene encoding a protein homologous to prokaryotic disulphide oxidoreductases. | 1995 | 7637700 | |
| a multitude of circular dnas exist in the nucleus of entamoeba histolytica. | 1995 | 7637704 | |
| dispar makes its debut. | 1995 | 7639254 | |
| purification and molecular cloning of a major antibacterial protein of the protozoan parasite entamoeba histolytica with lysozyme-like properties. | a protein with potent antibacterial activity was purified to apparent homogeneity from pathogenic entamoeba histolytica. it resembles lysozyme in that it is a basic protein which degrades cell walls of micrococcus luteus, displays optimal activity at acidic ph, and shows a preference for gram-positive bacteria. the protein has a molecular mass of approximately 23 kda upon sds/page and is localized inside the cytoplasmic granules of the amoebae. the primary structure was elucidated by protein ana ... | 1995 | 7649184 |
| amino acid consumption by the parasitic, amoeboid protists entamoeba histolytica and e. invadens. | amino acid consumption by entamoeba histolytica and e. invadens has been measured in order to assess the possible roles of amino acids as energy substrates. mixtures of amino acids enhanced the growth of the parasites in complex medium and their survival in simple medium. the consumption of several amino acids by the parasites suspended in simple media was greater when glucose was absent, suggesting that they may act as alternative energy sources. under these conditions, asparagine was consumed ... | 1995 | 7649448 |
| [the complement fixation reaction: a traditional, updated method for the detection of antibodies in the diagnosis of infections]. | complement-fixation (cf) is still an important basic serologic test for the diagnosis of infectious diseases. in several areas of microbiology (viral, bacterial, parasitic and fungal) it has served as a reference standard against which other methods have been compared. its partial displacement by other techniques, as advocated in some recent literature, is often unfounded and uncritical; displacement is mostly due not to problems inherent in the method, but rather to the lack of reagents of sati ... | 1995 | 7651069 |
| evaluation of lacto-phenol cotton blue for wet mount preparation of feces. | the use of lacto-phenol cotton blue (lpcb) stain for wet mount preparation of stools to demonstrate intestinal parasites by routine microscopy was evaluated in this study. lpcb-stained trophozoites and cysts and helminthic ova could easily be detected and identified in lpcb wet mounts of stools. the stain is recommended for routine use in the wet mount preparation of stools in a parasitology laboratory. | 1995 | 7540624 |
| amebiasis. | 1995 | 7548493 | |
| ancient weapons: nk-lysin, is a mammalian homolog to pore-forming peptides of a protozoan parasite. | 1995 | 7553868 | |
| effect of single dose of secnidazole in treatment of intestinal amoebiasis. | 1995 | 7474292 | |
| galactose-specific adhesion mechanisms of entamoeba histolytica: model for study of enteric pathogens. | 1995 | 7476406 | |
| purification and characterization of galactose- and n-acetylgalactosamine-specific adhesin lectin of entamoeba histolytica. | 1995 | 7476421 | |
| sequence and organization of an unusual histone h4 gene in the human parasite entamoeba histolytica. | 1995 | 7477106 | |
| stable episomal transfection of entamoeba histolytica. | 1995 | 7477110 | |
| disaccharidase deficiencies in mongolian gerbils (meriones unguiculatus) protected against giardia lamblia. | the activities of the disaccharidases lactase, maltase, sucrase and trehalase were examined in gerbils during giardia lamblia infections. in a primary infection with trophozoites, the activities of all four enzymes were reduced from day 10 post-infection (p.i.) and remained at low levels well past the elimination phase of the infection. however, during a challenge infection, the disaccharidase decreases were short-lived, with impairments being seen only on days 2 and/or 4 post-challenge (p.c.). ... | 1995 | 7479650 |
| effects of chaparrin, nordihydroguaiaretic acid and their structural analogues on entamoeba histolytica cultures. | 1995 | 7479999 | |
| in vitro cytotoxicity of chaparrin from castela texana. | 1995 | 7480018 | |
| immunogenicity of the recombinant serine rich entamoeba histolytica protein (srehp) amebiasis vaccine in the african green monkey. | we report the first study in non-human primates of the safety and immunogenicity of a recombinant vaccine designed to prevent amebic liver abscess. in a pilot study, a recombinant vaccine containing the serine rich entamoeba histolytica protein (srehp) attached to a maltose binding protein (srehp/mbp), which has been shown to be effective in preventing amebic liver abscess in rodent models of infection, was used to immunize two african green monkeys. vaccination with srehp/mbp resulted in no sys ... | 1995 | 7483769 |
| community survey of diarrhoea in children under 5 years in kinshasa, zaire. | this community based survey was undertaken to assess the prevalence, characteristics and risk factors of diarrhoea in children < 5 years of age in an urban zone, at kinshasa, zaire. 155 community cases selected by cluster sampling, 155 age-matched controls, and 18 children with diarrhoea seen at a health centre (hc) were examined. the diarrhoea prevalence rate was 6.5%. the highest risk of persistent diarrhoea was in children of 2 to 3 years, non breast-fed, with more than one enteric agent in t ... | 1995 | 7487197 |
| yi-s, a casein-free medium for axenic cultivation of entamoeba histolytica, related entamoeba, giardia intestinalis and trichomonas vaginalis. | pancreatic digests of casein are major ingredients of media used in the axenic cultivation of lumen-dwelling parasitic protozoa, especially entamoeba, giardia, and trichomonads. the digest used almost exclusively in the development of these media, medo-peptone (trypticase bbl), has not been available since 1981. moreover, none of dozens of similar type digests tested since then in our laboratory has proved equal to medo-peptone, and in the last two years it has become increasingly difficult to o ... | 1995 | 7496385 |
| dose- and time-dependent ultrastructural and functional lesions of the colonic mucosa by entamoeba histolytica lysates. | 1995 | 7502821 | |
| electrophysiologic and morphologic effects of entamoeba histolytica lysates on rabbit colon. | 1995 | 7502932 | |
| specific antiamebic serum antibodies in mexican puerperae and their newborns. | 1995 | 7502933 | |
| specific anti-e. histolytica iga monoclonal antibodies. | 1995 | 7502936 | |
| asymptomatic salmonella, shigella and intestinal parasites among primary school children in the eastern province. | the main objective of this study was to determine the prevalence of some potential entropathogens among primary school children. | 1995 | 23012224 |
| treatment of symptomatic amebic colitis in human immunodeficiency virus-infected persons. | while most entamoeba histolytica appearing in male homosexuals infected with human immunodeficiency virus (hiv) is considered non-invasive in western countries, and treatment of amebiasis in these persons has received very little attention, in japan some male homosexual amebiasis patients infected with hiv complain of symptoms attributable to e. histolytica infection. we investigated whether symptomatic e histolytica amebic colitis in hiv-infected persons requires higher doses or longer duration ... | 1996 | 18611760 |
| surface localization, regulation, and biologic properties of the 96-kda alcohol/aldehyde dehydrogenase (ehadh2) of pathogenic entamoeba histolytica. | the 96-kda surface antigen of entamoeba histolytica was demonstrated through extensive immunologic evaluation with monoclonal and monospecific antibodies to be identical to or an isoform of the amebic alcohol/aldehyde dehydrogenase (ehadh2). ehadh2 was secreted, excreted, or shed into the culture medium in quantities commensurate with amebic growth when studied in a novel culture system. of importance, using rnase protection assays, specific mrna coding for the ehadh2 gene product(s) was up-regu ... | 1996 | 8537663 |
| purification and characterization of the acetate forming enzyme, acetyl-coa synthetase (adp-forming) from the amitochondriate protist, giardia lamblia. | giardia lamblia, an amitochondriate eukaryote, contains acetyl-coa synthetase (adp-forming), an enzyme known only from one other eukaryote (entamoeba histolytica) and a few anaerobic prokaryotes. the enzyme has been purified about 350-fold. the activity in the direction of acetate formation was dependent on adp and inorganic phosphate. the reverse reaction could not be detected. succinyl-coa, propionyl-coa and dadp were utilized with lower efficiency. the enzyme did not utilize amp plus ppi thus ... | 1996 | 8557109 |
| the sequence and organization of the core histone h3 and h4 genes in the early branching amitochondriate protist trichomonas vaginalis. | among the unicellular protists, several of which are parasitic, some of the most divergent eukaryotic species are found. the evolutionary distances between protists are so large that even slowly evolving proteins like histones are strongly divergent. in this study we isolated cdna and genomic histone h3 and h4 clones from trichomonas vaginalis. two histone h3 and three histone h4 genes were detected on three genomic clones with one complete h3 and two complete h4 sequences. h3 and h4 genes were ... | 1996 | 8995053 |
| phagocytes from both vertebrate and invertebrate species use "coiling" phagocytosis. | coiling phagocytosis has been observed previously only by chance, and there has been no systematic investigation of this uptake mechanism. therefore, a comparative electron microscopical study was performed. different human and murine cell populations, phagocytes from various vertebrate and invertebrate species, and predatory amoebae were incubated with borrelia burgdorferi, one of the microbes known to induce coiling phagocytosis, to study the uptake mechanisms used. in this model, coiling phag ... | 1996 | 9040982 |
| prevalence of intestinal parasites in adult patients with enteropathic aids in north-eastern tanzania. | a six month study was conducted in north-eastern tanzania to determine the prevalence of pathogenic intestinal parasites among adult patients with enteropathic aids. a total of 352 patients were recruited of whom 158 (45%) had chronic diarrhoea. of the 352 patients, 123 (35%) had intestinal parasites. of the 123, 77 (62.6%) patients had chronic diarrhoea. the types of parasites detected were cryptosporidium, isospora belli, strongyloides stercoralis, schistosoma mansoni, trichuris, trichiura, as ... | 1996 | 8840602 |
| incidence of protozoal diarrheal disease in an expatriate community in addis ababa. | the incidence of diarrheal disease due to entamoeba histolytica and giardia lamblia in the united states embassy american population was recorded over a 25 month period. overall case rates of 4.2/100 person-months for amoebiasis and 3.3/100 person-months for giardiasis were distinguished by wide variations based on age group and rainfall. in some circumstances, gender and prior living in africa also correlated with disease incidence. practitioners should look for these entities in american expat ... | 1996 | 8840612 |
| amoebiasis of the female genital tract: a case report. | in 1994, a 56-year-old burmese woman presented with bloody discharge per vaginam had undergone pelvic examination disclosing multiple small ulcerated and friable lesions with contact bleeding at vulva, vagina and cervix. wet smears from vaginal discharge showed typical entamoeba histolytica trophozoites. the patient was treated with metronidazole 750 mg three times daily for 7 days, resulting in complete recovery of the lesions in 10 days. the pathology, risk factors, diagnosis and management of ... | 1996 | 8840708 |
| a single-step immunochromatographic test for the detection of entamoeba histolytica antigen in stool samples. | a rapid, single step, sensitive and specific immunochromatographic test for the detection of e. histolytica antigens in the stool samples is described. monoclonal antibody ac55 specific for e. histolytica was used as capture antibody on nitrocellulose sheet. the same antibody was conjugated to colloidal gold to detect the presence of antigen. in this test, multiple steps of washing, incubation, etc. are not required and there is no need for secondary colour development as gold particles produce ... | 1996 | 8841457 |
| protection against amebic liver abscess formation in the severe combined immunodeficient mouse by human anti-amebic antibodies. | we have used serum from patients with amebic liver abscess to investigate the role of antibody in the prevention of invasive amebiasis using the severe combined immunodeficient (scid) mouse model of entamoeba histolytica infection. the scid mice were passively immunized with serum or purified antibody from patients with amebic liver abscess 24 hr prior to the direct intrahepatic challenge with 10(6) virulent e. histolytica trophozoites. this treatment reduced the mean abscess size in these anima ... | 1996 | 8842124 |
| cytology of gastrointestinal histoplasmosis. a report of two cases with differential diagnosis and diagnostic pitfalls. | gastrointestinal (gi) histoplasmosis is a rare manifestation of histoplasma capsulatum (hc) infection. there are no reports of its cytologic diagnosis in the literature. | 1996 | 8842180 |
| use of the prospect microplate enzyme immunoassay for the detection of pathogenic and non-pathogenic entamoeba histolytica in faecal specimens. | a commercial microplate enzyme immunoassay (prospect eia; alexon inc., sunnyvale, ca 94089, usa) was compared with conventional microscopy for the diagnosis of entamoeba histolytica infection. using specimens known to be infected, the sensitivity of the prospect eia was 78% and its specificity was 99%. no cross reaction with other intestinal parasites was observed. the prospect eia and conventional microscopy (using merthiolate-iodine-formaldehyde direct wet mounts and concentration techniques) ... | 1996 | 8758065 |
| rapid in vitro test for determination of anti-amoebic activity. | a rapid microplate assay for the detection of potential compounds active against entamoeba histolytica is described. the assay is based on the metabolic reduction of a tetrazolium salt by e. histolytica trophozoites as an indicator of their viability which may be measured photometrically. the method was validated by determining the dose-response characteristics of standard amoebicides and correlating optical density and cell number; it provides a convenient means of selecting potentially novel a ... | 1996 | 8761587 |
| entamoeba histolytica: fecal antigen capture immunoassay for the diagnosis of enteric amebiasis by a monoclonal antibody. | amebiasis continues to be of epidemiological importance in underdeveloped countries. clinical diagnosis and epidemiological setting in a region are based on the fecal microscopic identification of cysts or trophozoites. this procedure requires well trained personnel, is laborious, of low sensitivity and frequently yields false-positives results. the present study was designed to develop an immunoenzymatic fecal 96 kda antigen capture test (coproelisa-eh) more sensitive and specific than microsco ... | 1996 | 8762638 |
| [the absence of invasive amebiasis in male homosexual aids patients in recife]. | the purpose of this paper was to determine if among male homosexual aids [correction of sida] patients, the entamoeba histolytica was able to product invasive illness. from the 77 investigated patients only one (1.3%) has cysts of e. histolytica in his stools. the electrophoresis of the only isolated strain has shown it was from zimodeme i, non pathogenic. the research of antiamebic antibodies was negative in the serum of the totality of patients. those results showed that even in immunocompromi ... | 1996 | 8768579 |
| production and characterization of murine monoclonal antibodies to blastocystis hominis. | several hybridomas producing antibodies detected by enzyme-linked immunosorbent assay (elisa) were established by fusions of mouse myeloma p3.x63.ag8.u1 with spleen cells from balb/c mice immunized against an isolate of blastocystis hominis. five strongly positive hybrids (6b6, 1d5, 1e7, 4f7 and 4g11) were cloned and all were found to secrete igm monoclonal antibodies. four mabs (6b6, 1e7, 4f7 and 4g11) reacted in immunoblots with a number of b. hominis antigens (mol. wt ranging from 25,000 to 2 ... | 1996 | 8773525 |
| synthesis and biological activity of platinum group metal complexes of o-vanillin thiosemicarbazones. | o-vanillin-(4-methylthiosemicarbazone), o-vanillin-(4-phenylthiosemicarbazone) and some of their metal complexes of the platinum group have been synthesized, characterized by chemical and spectral methods and studied for their antibacterial, antifungal and amoebicidal activity in vitro. the platinum group metal chelates exibited significant activity against a wide spectrum of microorganisms at different concentrations. the pt(ii) and ru(iii) chelates derived from o-vanillin-(4-phenylthiosemicarb ... | 1996 | 9050213 |
| [cytochemical study on the activity of enzymes in trophozoites of entamoeba histolytica from the cecum of guinea pigs with invasive and non-invasive amebiasis]. | experimental amoebiasis was inflicted on guinea pigs using the ps-2 strain of entamoeba histolytica. cytoenzymatic studies were conducted on the trophozoites sampled from the caecum of hosts with non-invasive and invasive amebiasis. the results show higher intensity of the processes of intracellular digestion, transportation, and anaerobic respiration in the amoebae originating from intestine of the host with infective amoebiasis. | 1996 | 9103051 |
| [acute epigastric pain after travel to the tropics. emergency admission of a 28-year-old stewardess]. | a 28-year-old stewardess was admitted to hospital with violent cramp-like pain in the right upper abdomen, which was accompanied by a single episode of watery pasty diarrhea. over the next few days her symptoms persisted, she developed a high fever, and her general state of health deteriorated. ultrasonography repeated on the fourth day then revealed a small space-consuming lesion in the right lobe of the liver. while the laboratory investigations initially remained negative, the suspected amebi ... | 1996 | 9119348 |
| [comparative study on serological diagnosis of entamoebiasis histolytica]. | the practicability of four diagnostic methods for entamoebiasis histolytica including micro-gel diffusion precipitin test (mgdp), indirect immunofluorescent antibody test (ifa), indirect hemagglutination test (iha) and enzyme-linked immunosorbent assay (elisa) was evaluated. the serological test methods were compared by using sera obtained from 30 entamoebiasis histolytica patients and 130 normal health individuals. the highest sensitivity was obtained with the method of elisa, followed by ifa a ... | 1996 | 8847819 |
| [recent features of parasites detected from clinical specimens]. | it is generally considered that we have already been through with problems caused by various kinds of parasites which had once raged throughout the country. on the contrary to our common concept, we occasionally encounter some kinds of parasites in a laboratory as well as in clinical fields, which have become unfamiliar to us in these days. parasitic diseases are in the first place, proper and limited to certain local regions, but the present situation has been greatly changed. nowadays, specifi ... | 1996 | 8847822 |
| diagnostic tests for amoebic liver abscess: comparison of enzyme-linked immunosorbent assay (elisa) and counterimmunoelectrophoresis (cie). | the liver abscess is the most frequent extraintestinal complication of intestinal amoebiasis: its diagnosis is suggested by the clinical picture but it must be confirmed by paraclinic tests. themost stringent diagnosis requires identification of e. histolytica. but this is possible only in a few cases. serological tests greatly improve the diagnosis of this severe complication of amoebiasis. we compared the enzyme linfed immunosorbent assay and the counterimmunoelectrophoresis techniques. both t ... | 1996 | 8851212 |
| energy production in entamoeba histolytica: new perspectives in rational drug design. | the amebicidal action of metronidazole is activated when the enzyme pyruvate:ferredoxin oxido-reductase transfers reducing equivalents to the nitro group of the drug. the enzyme is present in entamoeba histolytica and other anaerobic parasites like giardia and trichomonas that lack mitochondria. the selectivity of the drug can be ascribed to the absence of the reductase in the human host. e. histolytica possesses other enzymes involved in glucose catabolism that are interesting for the rational ... | 1996 | 8854380 |
| the ultrastructure of entamoeba histolytica locomotion. | this quantitative ultrastructural survey of e. histolytica locomotion in boyden chambers supports the concept that this parasite is capable of random, chemokinetic and chemotactic motility. an e. histolytica committed to chemotaxis will flatten over the filter, accumulate smaller vacuoles at the front of the cell, and will also project pseudopods and its polarized body towards and alongside the chemoattractant axis, respectively. other cell features such as cell polarization, membrane ruffling, ... | 1996 | 8854387 |
| molecular biology of entamoeba histolytica: a review. | amebiasis is one of the main causes worldwide of morbidity and mortality by parasites. application of recombinant dna technology to the study of entamoeba histolytica is bringing new light into our understanding of this remarkable protozoan parasite and of the disease it causes. new achievements affect the way we approach many essential questions about e. histolytica, from the mechanism of its pathogenicity to the definition of e. histolytica as a separate species from the nonpathogenic e. dispa ... | 1996 | 8854402 |
| physiology and molecular biology of multidrug resistance in entamoeba histolytica. | in this paper, we present the most relevant facts on multidrug resistance (mdr) in the protozoan parasite entamoeba histolytica. mdr in e. histolytica presents characteristics similar to transformed mammalian cells. e. histolytica drug resistant mutants show cross-resistance to several drugs, and as in mammalian cells the resistance is reverted by verapamil. six p-glycoprotein-like genes (ehpgp) have been cloned and characterized. apparently, four of these genes are transcribed in drug-resistant ... | 1996 | 8854404 |
| protozoa. amebiasis. | the intestinal protozoan parasite entamoeba histolytica causes amebic dysentery and amebic liver abscess, and ranks third worldwide among parasitic causes of death. the application of molecular techniques to the study of this organism have led to major advances in understanding the pathophysiology of amebic infection. this article reviews what is currently known about the pathogenesis, clinical manifestations, diagnosis, and treatment of amebiasis. | 1996 | 8863036 |
| differentiation of entamoeba histolytica and e. dispar dna from cysts present in stool specimens by polymerase chain reaction: its field application in the philippines. | it has been established that two distinct species exist within what was originally known as entamoeba histolytica. these are e. dispar and e. histolytica, for the nonpathogenic and pathogenic forms, respectively. differentiation of these two organisms is of great clinical importance since they are morphologically indistinguishable and both forms can infect the human intestinal cavity to different degrees. a simple and rapid dna-extraction method that can be used directly on formalin-fixed stool ... | 1996 | 8875564 |
| immunodominant entamoeba histolytica antigens recognized by serum and intestinal antibodies after local or systemic immunization of mice with glutaraldehyde fixed trophozoites. | we performed an immunoblot analysis of the main e. histolytica proteins recognized by immune sera and intestinal fluids of balb/c mice immunized with glutaraldehyde fixed trophozoites (gft) by intragastric, rectal and intraperitoneal routes, to determine if there were differences in the amebic antigens immunodominantly recognized at mucosal and systemic levels. the antigen patterns recognized by mice immunized via intraperitoneal and rectal routes were complex and similar suggesting that the imm ... | 1996 | 8876658 |
| entamoeba invadens: characterization of cysteine proteinases. | cysteine proteinases have a number of important functions in the life cycle of protozoan parasites. based on our previous studies demonstrating the role of cysteine proteinases in invasion by entamoeba histolytica, we evaluated the cysteine proteinases of e. invadens, a related protozoan which causes invasive disease of reptiles. e. invadens readily encysts in axenic culture and provides a model to investigate the role of cysteine proteinases in encystation. broad bands of approximately 130-230, ... | 1996 | 8888735 |
| putative serine/threonine protein kinase expressed in complement-resistant forms of entamoeba histolytica. | entamoeba histolytica is susceptible to complement attack in its lumen-dwelling state and develops complement resistance during pathogenic tissue invasion. as experimental evidence suggests that this change in phenotype is accompanied by a change in gene expression, we constructed a subtractive cdna library to identify genes involved. poly(a) + rna from complement-sensitive trophozoites was subtracted from single stranded cdna derived from complement-resistant ones. transcripts enriched in the l ... | 1996 | 8892294 |
| cell surface molecules of pathogenic and nonpathogenic entamoeba histolytica and their relation to virulence. | 1996 | 8895796 | |
| phylogenetic position of kinetoplastid protozoa inferred from the protein phylogenies of elongation factors 1alpha and 2. | partial regions of the mrna encoding a major part of translation elongation factor 2 (ef-2) from a kinetoplastid protozoan, trypanosoma cruzi, were amplified by means of polymerase chain reaction and their primary structures were analyzed. the deduced amino acid sequence was aligned with those of other eukaryotic and archaebacterial ef-2s, and the phylogenetic relationships among eukaryotes were inferred by the maximum likelihood (ml) method. ml analyses of ef-2 phylogeny using six different sto ... | 1996 | 8907178 |
| identification of a 35 kda glycoprotein from entamoeba histolytica by sera from patients with amoebic liver abscess and with mouse monoclonal antibody. | in this work, we explored the relevance of a 35 kda glycoprotein (gm) of the outer membrane from e. histolytica in the diagnosis of the amoebic liver abscess (ala) through elisa and immunoblotting. we were interested in defining the relevance of this antigen in the immune response in patients with amoebic liver abscess and in exploring whether the mouse monoclonal antibody against this 35 kda glycoprotein recognises the same epitope. we found that 87% of ala patients had raised antibody levels t ... | 1996 | 8915688 |
| parasitological and bacteriological studies in recurrent diarrhoea in patients with chronic liver diseases. | sixty patients with chronic liver disease complaining of persistent or recurrent diarrhoea where studied. the stools were examined by direct smear, formol ether concentration method and modified ziehl neelsen technique for cryptosporidium in addition to stool cultures for enteric pathogenic bacteria as salmonella, shigella and e. coli. giardia lamblia was the most prevalent pathogen responsible for diarrhoea in 23.3% of cases followed by entamoeba histolytica in 21.6%, schistosoma mansoni in 5% ... | 1996 | 8918043 |
| evaluation of an antigen-capture enzyme immunoassay for detection of entamoeba histolytica in stool samples. | in order to identify the prevalence of entamoeba histolytica in tourists with diarrhoea returning from countries of the developing world, sensitivity and specificity of a commercially available enzyme immunoassay (eia) kit for the detection of entamoeba histolytica coproantigen in stool were evaluated. five hundred seventy-seven specimens from 469 patients were examined by microscopy and eia. sixty-two specimens from 49 patients were considered positive for entamoeba histolytica. compared with m ... | 1996 | 8922579 |
| [amebic liver abscess--personal observations]. | in the period of 1989-1995 seven patients with amebic liver abscess were observed in clinic of infectious diseases of pomeranian medical school in szczecin. the diagnosis has been made on the base of epidemiological data, presence of intrahepatic defect by a scanning procedure of liver (ultrasonography, ct, scintigraphy) and positive serologic test for amebiasis. all patients were male of polish nationality, 29-57 years old, who became ill after travel to africa or india. intestinal amebiasis wa ... | 1996 | 8927739 |
| entamoeba histolytica and entamoeba dispar: differences in numbers and expression of cysteine proteinase genes. | in order to identify molecules that might be responsible for the difference in pathogenicity between the two closely related protozoan parasites entamoeba histolytica and entamoeba dispar, we focussed on cysteine proteinases because this class of enzymes has been considered important for pathogenic tissue destruction. by screening a genomic library derived from an e. histolytica isolate, a total of six distinct genes (ehcp1-ehcp6) encoding typical prepro-forms of cysteine proteinases were identi ... | 1996 | 8930910 |
| codon usage in cryptosporidium parvum differs from that in other eimeriorina. | codon usage of crytosporidium parvum was compared with those of other eimeriorina toxoplasma gondii and eimeria tenella and revealed a biased use of synonymous codons with a preference for nnu (40.0%) and nna (33.4%). there was no close resemblance of the codon usage of c. parvum to t. gondii (correlation coefficient, r = 0.14) or e. tenella (r = 0.14) but it was similar to entamoeba histolytica (r = 0.75) and plasmodium falciparum (r = 0.5). analysis of the codon usage in homologous gene sequen ... | 1996 | 8935947 |
| direct sequencing of the pcr amplified ssu rrna gene of entamoeba dispar and the design of primers for rapid differentiation from entamoeba histolytica. | since 1993, strains of entamoeba histolytica sensu lato have been assigned to 2 species on the basis of clinical, biochemical, immunological and genetic evidence: the pathogenic strains to e. histolytica sensu stricto, the non-pathogenic strains to entamoeba dispar. analysis of the gene encoding for the small subunit ribosomal rna (ssu rdna) supports the existence of 2 species. however, while 3 whole ssu rdna sequences are available in the data bases for e. histolytica, only a partial sequence h ... | 1996 | 8935948 |
| interaction of ls174t human colon cancer cell mucins with entamoeba histolytica: an in vitro model for colonic disease. | colonic mucins secreted by goblet cells protect the colon by preventing the attachment of enteric pathogens to the epithelium. entamoeba histolytica overcomes this protective barrier and causes ulcerations, allowing the parasite to disseminate to the liver and form abscesses. an in vitro model is used to study the interaction between e. histolytica and colonic mucins. | 1996 | 8942726 |
| characterization of ubiquitin genes and -transcripts and demonstration of a ubiquitin-conjugating system in entamoeba histolytica. | we have investigated the genomic organization of entamoeba histolytica ubiquitin and looked for the occurrence of a ubiquitin-conjugating system in this organism. southern blots indicated the presence of > or = 5 ubiquitin-coding regions. one of these, ehubi1, was cloned and sequenced and found to correspond to a monoubiquitin gene; as shown by a polymerase chain reaction, e. histolytica lacked polyubiquitin genes altogether. blots of poly(a)+ rna from exponentially-growing trophozoite cultures ... | 1996 | 8943152 |
| pacsr: a serum replacement for axenic cultivation of entamoeba histolytica. | 1996 | 8944281 | |
| luminal bacteria and proteases together decrease adherence of entamoeba histolytica trophozoites to chinese hamster ovary epithelial cells: a novel host defence against an enteric pathogen. | factors that prevent colonic mucosal invasion by pathogenic entamoeba histolytica are not understood. a key initial step in pathogenesis of injury induced by amoeba is adherence to target cells mediated by a surface glycoprotein lectin on e histolytica. mucin degrading bacteria normally present in the colon lumen produce glycosidases that degrade soluble or cell surface glycoconjugates. | 1996 | 8944559 |
| overexpression of p-glycoprotein gene 1 by transfected entamoeba histolytica confers emetine-resistance. | 1996 | 8946391 | |
| trypanosoma rangeli and trypanosoma cruzi: molecular characterization of genes encoding putative calcium-binding proteins, highly conserved in trypanosomatids. | genes encoding a 29-kda flagellar calcium-binding protein (f29) in trypanosoma cruzi, strongly homologous to ef-hand calcium-binding protein-encoding genes previously reported in this parasite, were isolated by immunoscreening. f29 is encoded by a number of very similar genes, highly conserved among different t. cruzi isolates. the genes are located on a pair of homologous chromosomes, arranged in one or two clusters of tandem repeats. pcr amplification of trypanosoma rangeli genomic dna, using ... | 1996 | 8948328 |
| presence of a mitochondrial-type 70-kda heat shock protein in trichomonas vaginalis suggests a very early mitochondrial endosymbiosis in eukaryotes. | molecular phylogenetic analyses, based mainly on ribosomal rna, show that three amitochondriate protist lineages, diplomonads, microsporidia, and trichomonads, emerge consistently at the base of the eukaryotic tree before groups having mitochondria. this suggests that these groups could have diverged before the mitochondrial endosymbiosis. nevertheless, since all these organisms live in anaerobic environments, the absence of mitochondria might be due to secondary loss, as demonstrated for the la ... | 1996 | 8962101 |
| heterogeneity of entamoeba histolytica rac genes encoding p21rac homologues. | entamoeba histolytica (eh), the parasite that causes amebic dysentery, is the only protozoan that phagocytoses bacteria, epithelial cells and red blood cells. numerous low-molecular weight gtp-binding proteins, called p21rac, are implicated in signal transduction and actin polymerization during phagocytosis by macrophages and dictyostelium discoideum (dd). here, molecular cloning techniques were used to obtain four eh rac genes that encoded putative p21rac, as well as segments of two eh rac pseu ... | 1996 | 8964500 |
| identification and cellular localization of the actin-binding protein abp-120 from entamoeba histolytica. | several actin-binding proteins participate in the morphological changes that occur during amoeboid movement. the gene encoding one of these proteins, the gelation factor abp-120, was identified and characterized from trophozoites of entamoeba histolytica. the sequence contains 2574 nucleotides, with an open reading frame of 858 amino acids, giving a protein of 93 kda belonging to the spectrin family. the n-terminal domain of abp-120 from e. histolytica revealed a consensus site for actin binding ... | 1996 | 8971707 |
| identification of novel genes from entamoeba histolytica by expressed sequence tag analysis. | shotgun sequencing of cdna clones is now an established approach to gain insight into the expressed nucleotide (nt) sequences in a given cell. we analysed 100 randomly picked cdna clones of the protozoan parasite, entamoeba histolytica, by nt sequencing, with a view to obtain novel gene sequences not detected so far by biochemical and genetic analyses. about 56% of the analysed clones showed significant homology with other genes in the database, including a number of genes whose presence may not ... | 1996 | 8973317 |
| identification and analysis of the u6 small nuclear rna gene from entamoeba histolytica. | among the small nuclear rnas (snrnas) involved in the spliceosomal processing of pre-mrna, u6 is the most conserved. as a first evidence for the presence of the splicing machinery in the amitochondrial protozoan entamoeba histolytica (eh), we have cloned the u6 snrna gene. we find that in this organism u6 is a single copy gene that is transcribed as a poly(a)- rna molecule of approximately 105 nucleotides. we have mapped the 5' end of the u6 snrna transcript, and identified typical elements of a ... | 1996 | 8973344 |
| persistent diarrhea caused by isospora belli: therapeutic response to pyrimethamine and sulfadiazine. | a 54-year-old human immunodeficiency virus (hiv)-positive homosexual man developed overwhelming watery diarrhea and marked weight loss over a 3-week period. although entamoeba histolytica and other nonpathogenic enteric protozoa were observed along with isospora belli in this patient's stool specimens, they were promptly eradicated after metronidazole (flagyl) treatment. the presence of i. belli oocysts in various stages of development in the stool and clinical symptoms related to isospora infec ... | 1996 | 8985661 |
| comparable growth of a trichomonas vaginalis strain in pehps and tyi-s-33 media. | pehps medium, developed for axenic cultivation of entamoeba histolytica and e. invadens, was also capable of supporting the growth of a trichomonas vaginalis strain, with an inoculum of 1 to 100 trichomonads/ml. the logarithmic growth phase in pehps or in tyi-s-33 medium lasted 72 h; yield (3.33 +/- 0.56 x 10(6) trichomonads/ml), duplication time (4.27 h), number of duplications (16.85), or increase ratio (33,328) in pehps medium showed no significant differences with those obtained in tyi-s-33 ... | 1996 | 8987196 |
| antioxidant defense mechanisms in parasitic protozoa. | many of the parasitic protozoa, such as entamoeba histolytica, giardia, trypanosoma, leishmania, and plasmodium, are considered to be anaerobes because they can be grown in vitro only under conditions of reduced oxygen tension. however, these parasitic protozoa have been found to be aerotolerant or microaerophilic, and also to consume oxygen to a certain extent. furthermore, these organisms are highly susceptible to exogenous reactive oxygen species, such as hydrogen peroxide. they must, therefo ... | 1996 | 8989515 |
| evidence for the existence of ganglioside molecules in the antigen of entamoeba histolytica. | gangliosides were found to be present in entamoeba histolytica. they were extracted from lyophilized trophozoites of the pathogenic strain hm-1:imss and purified by high performance thin-layer chromatography. two resorcinol-positive bands, comigrating with gm2 and gd1a were demonstrated, revealing the existence of ganglioside molecules in entamoeba histolytica. the gm2 content, determined as lipid-bound sialic acid, was 1.5 micrograms/10(8) amoebae, the content of the gd1a comigrating band was 0 ... | 1996 | 9223167 |
| [seasonality of parasites and intestinal bacteria in vegetables that are consumed raw in costa rica]. | in costa rica, a total of 640 samples from eight different vegetables used for raw consumption, were analyzed for the presence of intestinal parasites and fecal coliforms. eighty samples of each vegetable were analyzed, forty during the dry season and forty in the rainy. a greater, but unsignificant (p > 0.05) level of fecal coliforms was found during the dry season. levels of escherichia coli, were higher (p < 0.05) during the dry season in lettuce (latuca sativa) and cilantro (coleandrum sativ ... | 1996 | 9246361 |
| [diarrhea and aids: more complex diagnostic techniques; better therapeutic results]. | during 17 months, 73 hiv-positive patients with diarrhea lasting at least for 14 days, were studied prospectively. the patients had stool specimen examinations negative for enteric pathogens, or positive for one of them, but with no response to specific treatment. all patients were subcomitted to digestive endoscopy and biopsies were taken for microbiological and histological studies. the etiology of the diarrhea could be established in 48 patients (66%). in 45, the cause was an enteric infectio ... | 1996 | 9137663 |
| seroepidemiology of five major zoonotic parasite infections in inhabitants of sidoarjo, east java, indonesia. | we conducted a seroepidemiological survey of zoonotic parasite infection in inhabitants of east java, indonesia. the subjects of the survey were 244 persons selected from visitors to sidoarjo city hospital in east java between may 1992 and october 1993. ninety-seven had diarrhea and the rest came to the hospital for routine check-ups. all serum samples were tested for antibodies against five zoonotic parasites: toxoplasma gondii, entamoeba histolytica, toxocara canis. angiostrongylus cantonensis ... | 1996 | 9185269 |
| monoclonal antibody-based elisa for the detection of circulating entamoeba histolytica antigen in hepatic amebiasis in hamsters (mesocricetus auratus). | circulating amebic antigens were determined by using a sandwich elisa with specific monoclonal antibody in the sera of 35 group i hamsters, 7 of which were sacrificed at intervals after hepatic inoculation with 500,000 axenically grown hm:1:imss strain of e. histolytica trophozoites, 7 group ii infected hamsters in which metronidazole treatment was given and 18 group iii uninfected controls. amebic antigenemia was demonstrated in 5 of 7 (71.4%), 6 of 7 (85.7%), 7 of 7 (100%), 7 of 7 (100%) and 7 ... | 1996 | 9253880 |
| isoenzyme profile as parameter to differentiate pathogenic strains of entamoeba histolytica in brazil. | the isoenzyme profiles (ip) of 33 strains of entamoeba histolytica isolated from patients and carriers of two regions in brazil (amazonia and southeast) were determined. the enzymes phosphoglucomutase, glucose-phosphate isomerase, hexokinase and malic enzyme were considered. ip of the strains was correlated with culture conditions, time of maintenance in laboratory and clinical history of patients. the strains were maintained under polyxenic, monoxenic and axenic culture conditions: 27 polyxenic ... | 1996 | 9293086 |
| prevalence and etiology of intestinal parasites in lebanon. | as there are no studies done on the prevalence of intestinal parasites in lebanon since 1967, this study was undertaken to reveal the current patterns of intestinal parasitic infestations in lebanese patients from two geographic regions: beirut and tripoli. analysis was based on 33,253 stool specimens examined at the american university of beirut medical center (auh) and 11,611 specimens examined at the islamic hospital (ih) in tripoli over five and three years, respectively. the prevalence of i ... | 1996 | 9260400 |
| detoxicating enzymes of entamoeba histolytica and their detoxifying roles. | to investigate the entamoeba histolytica living in the low oxygen concentration colon of the host and how does it survive in the circumstance after invading the tissues with high oxygen concentration while obtaining oxygen without being damaged by the toxins. | 1996 | 9275357 |