Publications

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physiological characterization of antibiotic resistant mutants of bacillus subtilis. 19724622637
patterns of oxygen interchange between water, substrates, and phosphate compounds of escherichia coli and bacillus subtilis. 19724622742
kinetic complexity of rna molecules. 19724622766
comparison of procedures for extracting transfer rna from spores of bacillus. 19724622780
protein turnover in asporogenic bacillus megaterium km under limited nitrogen supply. 19724622790
stimulation of the development of competence by culture fluids in bacillus subtilis transformation. 19724622862
[the mechanism of action of alpha-amylase on amylose]. 19724622864
bacteriolytic enzymes produced by myxococcus xanthus.the bacteriolytic activities in the culture fluid of myxococcus xanthus were purified and separated into six active fractions by the use of bio-gel cm-2 and bio-gel p-60. these fractions were identified as: (i) an amidase, (ii) a glucosaminidase, (iii) a glucosaminidase and an amidase, (iv) a protease with probable amidase activity, (v) another protease with probable amidase activity, and (vi) a peptidase active on both d-alanyl-diaminopimelate and d-alanyl-lysine peptide bonds. on one occasion, ...19724622898
number of deoxyribonucleic acid uptake sites in competent cells of bacillus subtilis.two direct methods are presented for estimating the average number of deoxyribonucleic acid (dna) uptake sites in competent cells of bacillus subtilis from measurement of (14)c- or (3)h-thymine-labeled dna uptake by competent culture. advantage is taken of two facts: (i) effective contact between competent cells and transforming dna molecules is established within a short time after mixing them together, and (ii) dna molecules enter the competent b. subtilis cells in a linear fashion at a finite ...19724622899
regulation of the bacterial cell wall: analysis of a mutant of bacillus subtilis defective in biosynthesis of teichoic acid.bacillus subtilis 168ts-200b is a temperature-sensitive mutant of b. subtilis 168 which grows as rods at 30 c but as irregular spheres at 45 c. growth at the nonpermissive temperature resulted in a deficiency of teichoic acid in the cell wall. a decrease in teichoic acid synthesis coupled with the rapid turnover of this polymer led to a progressive loss until less than 20% of the level found in wild-type rods remained in spheres. extracts of cells grown at 45 c contained amounts of the enzymes i ...19724622900
initiation of spore germination in glycolytic mutants of bacillus subtilis.enzyme activities of glycolysis and glyconeogenesis are present in spores of bacillus subtilis, the rate-limiting step of glucose (glc) metabolism being its phosphorylation. glc allows initiation of germination in the presence of fructose (fru) and asparagine (asn), not because it is used via the embden-meyerhof path, but because it is oxidized in the nonphosphorylated form via the spore-specific glc dehydrogenase. spores of mutants lacking glc-phosphoenolpyruvate transferase, fru-6-p-kinase, or ...19724622901
aggregation of deoxyribonucleic acid and the helping effect in transformation.deoxyribonucleic acid aggregates in the presence of a component of neopeptone at low ionic strength and thereby loses its ability to bind to competent bacteria. the reversible and concentration-dependent nature of this aggregation is the basis of the helping effect in transformation.19724622904
mutagen stability of alkylation-sensitive mutants of bacillus subtilis.a series of mutations of bacillus subtilis, conferring sensitivity to methyl methanesulfonate (mms), were transferred by transformation to a suppressible his(-) stock. the introduction of certain sensitivity mutations prevented the ultraviolet- or mms-induced, but not the spontaneous, reversion of his(-) to his(+). not all sensitivity mutations led to this resistance to mutagenesis; a strain with altered deoxyribonucleic acid (dna) polymerase activity behaved almost normally with respect to its ...19724622905
characterization of s-adenosylmethionine: ribosomal ribonucleic acid-adenine (n 6 -) methyltransferase of escherichia coli strain b.this study is concerned with the isolation and characterization of the enzyme, s-adenosylmethionine:ribosomal ribonucleic acid-adenine (n(6-)) methyl-transferase [rrna-adenine (n(6)-) methylase] of escherichia coli strain b, which is responsible for the formation of n(6)-methyladenine moieties in ribosomal ribonucleic acids (rrna). a 1,500-fold purified preparation of the species-specific methyltransferase methylates a limited number of adenine moieties in heterologous rrna (micrococcus lysodeik ...19724622906
control of fatty acid synthesis in bacteria.when glycerol-requiring auxotrophs of bacillus subtilis are deprived of glycerol, the synthesis of fatty acids continues at an apparent rate of 20 to 50% that of supplemented cultures. the newly synthesized fatty acids are not incorporated into phospholipid and accumulate as free fatty acids. these molecules undergo a much more rapid turnover than phospholipid fatty acids, and the rate of turnover is sufficient to indicate that the rate of fatty acid synthesis in glycerol-deprived cultures is si ...19724622908
[asparaginase activity of some bacteria]. 19724622929
effect of triton x-100 on sweet potato -amylase. 19724622930
effect of caffeine on the recombination process of bacillus subtilis. 19724622966
the effect of laundry detergent containing b. subtilis enzyme in producing sensitization reactions in guinea pigs. 19724622992
[active metabolites of oxacillin]. 19724623086
amino acid control of messenger ribonucleic acid synthesis in bacillus subtilis. 19724623129
phi29 bacteriophage structural proteins. 19724623200
dominance relationships in mixedly infected bacillus subtilis.the progeny released from bacillus subtilis cells mixedly infected with bacteriophages beta22, sp82, and sp02(c1) have been studied at varying multiplicities of infection and orders of addition and with different host strains of the bacterium. in b. subtilis 168, sp02(c1) was subordinate to both sp82 and beta22 and did not yield significant numbers of progeny even when added 5 min before the superior phage. dominance in mixed infections of beta22 and sp82 was host-dependent. in b. subtilis 168, ...19724623223
new deoxyribonucleic acid polymerase induced by bacillus subtilis bacteriophage pbs2.the deoxyribonucleic acid (dna) of bacillus subtilis phage pbs2 has been confirmed to contain uracil instead of thymine. pbs2 phage infection of wild-type cells or dna polymerase-deficient cells results in an increase in the specific activity of dna polymerase. this induction of dna polymerase activity is prevented by actinomycin d and chloramphenicol. in contrast to the major b. subtilis dna polymerase, which prefers deoxythymidine triphosphate (dttp) to deoxyuridine triphosphate (dutp), the dn ...19724623224
a bacteriophage of bacillus subtilis which forms plaques only at temperatures above 50 c. 3. inhibition of tsp-1-specific deoxyribonucleic acid synthesis at 37 c and 45 c.the effect of temperature on phage-specific deoxyribonucleic acid (dna) synthesis was studied in tsp-1-infected bacillus subtilis. this was facilitated by selectively inhibiting host dna synthesis with 6-(p-hydroxyphenylazo)-uracil. the results indicated that tsp-1 dna synthesis did not continue at 37 c and was immediately shut down after transfer to this temperature. incubation at 45 c greatly reduced tsp-1 dna synthesis. phage-specific dna synthesis could resume at 53 c, however, when the infe ...19724623225
effect of no 2 on airborne venezuelan equine encephalomyelitis virus.studies were conducted to determine the effect of nitrogen dioxide (no(2)) on aerosol survival and biological decay rate of venezulean equine encephalomyelitis (vee) virus and spores of bacillus subtilis var. niger. the no(2) concentrations used in the experiments were 0.5, 5, and 10 ppm at 24 c and 85% rh. the survival of airborne vee virus disseminated as particles 1 to 5 mum in diameter was significantly influenced by the presence of 5 ppm of no(2). at this concentration, the biological decay ...19724623277
sporostatic and sporocidal properties of aqueous formaldehyde.aqueous formaldehyde is shown to exert both sporostatic and sporocidal effects on bacillus subtilis spores. the sporostatic effect is a result of the reversible inhibition of spore germination occasioned by aqueous formaldehyde; the sporocidal effect is due to temperature-dependent inactivation of these spores in aqueous formaldehyde. the physicochemical state of formaldehyde in solution provides a framework with which to interpret both the sporostatic and sporocidal properties of aqueous formal ...19724623282
comparison of deoxyribonucleic acid uptake and marker integration in bacilli and protoplasts of bacillus subtilis.trp(+)his(-) donor deoxyribonucleic acid (dna) was added to highly competent trp(-)his(+) recipient bacilli and to protoplasts prepared from these bacilli, and the cell-dna complexes were incubated for 30 min. the complexes were then washed and lysed, and their dna was analyzed on a trp(-)his(-) strain for the donor marker trp(+), the resident marker his(+), and for the recombinant trp(+)his(+) combination. the extracts of the bacillary complexes contained a normal percentage of donor markers (0 ...19724623311
[regulation of nucleotide biosynthesis]. 19724623403
replication of viral dna in spo1-infected bacillus subtilis. i. replicative intermediates. 19724623500
replication of viral dna in spo1-infected bacillus subtilis. ii. dna maturation during abortive infection. 19724623501
[binding sites of subtilisin]. 19724623542
[in vivo degradation of bacterial ribosomes and the occurrence of abnormal particles]. 19724623543
ultraviolet inactivation and excision-repair in bacillus subtilis. ii. differential inactivation and differential repair of transforming markers. 19724623568
ultraviolet inactivation and excision-repair in bacillus subtilis. i. construction and characterization of a transformable eightfold auxotrophic strain and two ultraviolet-sensitive derivatives. 19724623569
gentamicin therapy in renal failure: a nomogram for dosage. 19724623584
[metabolic products of microorganisms. 94. comparison of sideramines as growth factors and as antagonists of the sideromycins]. 19724623589
[metabolic products of microorganisms. 96. mechanism of action of the macrolide-type antibiotic, chlorothricin. i. inhibitor of the pyruvate carboxylase from bacillus subtilis]. 19724623590
trace elements for growth and bulbiformin production by bacillus subtilis. 19724623605
unrelatedness of temperate bacillus subtilis bacteriophages sp02 and phi105.spo2 and phi105 are temperate bacillus subtilis bacteriophages which have been suggested to belong to a cluster of related bacteriophages. in the present work, we show that spo2 does not complement any of the 11 essential genes known in phi105 and that the phages do not recombine. deoxyribonucleic acid (dna)-dna hybridization shows less than 10% homology between spo2 and phi105 dna. dna synthesis in phi105 shows a greater dependence on host functions than does spo2 dna synthesis. growth of phi10 ...19724623614
nucleic acid synthesis in bacteriophage spo2c 1 -infected bacillus subtilis.the synthesis of host macromolecules was shut off very slowly and incompletely by bacteriophage spo2c(1). no change in the rate of incorporation of radioactive precursors into protein and ribonucleic acid (rna) could be detected after infection, and the rate of incorporation of thymidine was increased only slightly. the relative proportions of phage and host species of nucleic acids at various intervals in the latent period were determined by means of nucleic acid hybridization. phage-specific r ...19724623615
pyruvate kinase of bacillus subtilis. 19724623707
the effect of penicillin on competence in bacillus subtilis cultures growing in chemostat at different doubling times. 19724623869
mechanism of biological action of a new benzindazole compound. 19724623882
binding and release from cell walls: a unique approach to the purification of autolysins. 19724624004
sterilization by electrohydraulic discharges. 19724624006
de novo protein synthesis during the development of competence in bacillus subtilis. 19724624011
[studies on chemotherapeutics. vii. on the antimicrobial activity and syntheses of halogen substituted-diphenylether compounds]. 19724624023
formation of a 4-nitroquinoline-i-oxide complex with dna in normal and repair deficient strains of bacillus subtilis. 19724624026
[fatal shock due to live culture of bacillus cereus]. 19724624046
the combined bactericidal effect of acetic acid and a moderate heat treatment in the preparation of a concentrated haemodialysis solution. 19724624095
buoyant density studies on natural and synthetic deoxyribonucleic acids in neutral and alkaline solutions. 19724624113
characterization of lysine transfer ribonucleic acid from vegetative cells and spores of bacillus subtilis. 19724624117
purification of the bacteriophage sp01 transcription factor 1. 19724624121
tolerance of staphylococcal thermonuclease to stress.remarkable tolerance to prolonged heating, prolonged storage, and bacterial proliferation was exhibited by staphylococcal thermonuclease in foods and broth. a purified enzyme preparation added to brain heart infusion broth was unaffected by the growth of five bacterial species. minimal inactivation was effected by bacillus subtilis. optimal growth of streptococcus faecalis var. liquifaciens caused extensive inactivation of thermonuclease. however, storage at room temperature or the addition of 5 ...19724624218
analysis of temperature-sensitive sporulation mutants of bacillus subtilis by transduction. 19724624394
characteristics of an aminopeptidase activity from the cultural fluid of bacillus subtilis. 19724624550
antibacterial properties of silver chelates of uracil and uracil derivatives in vitro. 19724624600
a new dipeptide antibiotic from streptomyces collinus, lindenbein. 19724624614
effect of tunicamycin on microorganisms: morphological changes and degradation of rna and dna induced by tunicamycin. 19724624615
discussion paper: bacterial transport mechanisms as studied in cytoplasmic membrane vesicles. 19724624659
the microbial flora of the gut of the pouch-young and the pouch of a marsupial, setonix brachyurus. 19724624672
air-borne b. subtilis enzymes in the detergent industry. 19724624735
ribosomal rna synthesis in vitro: a protein-dna complex from bacillus subtilis active in initiation of transcription.a protein-dna transcription complex, isolated from logarithmic-phase cells of bacillus subtilis, is active in the initiation of new rounds of rna synthesis in vitro. transcription directed by the endogenous dna of the complex is sensitive to inhibitors of initiation. in addition, the effect of addition of a competing template indicates that rna polymerase in the complex is bound to endogenous dna in a dissociable, preinitiation state. hybridization-competition analysis of the rna product obtaine ...19724624758
early extracellular events in infection of competent bacillus subtilis by dna of bacteriophage sp82g.analysis, by the recovery of specific genetic "markers," of the effects of dnase i, physical shear, and temperature shock on dna-cell complexes demonstrates that sequential attachment of both ends of bacteriophage sp82g dna to bacillus subtilis precedes entry of the dna molecule into the cell, and that each attachment is end-and time-specific. the first attachment involves an initial reversible phase, followed by irreversible binding. after a latent period, the second end then attaches to the ce ...19724624760
continuous production of extracellular protease by bacillus subtilis in a two-stage fermentor. 19724624771
positional distribution of fatty acids in phospholipids from bacillus subtilis. 19724624831
sporulation in bacillus subtilis. genetic analysis of oligosporogenous mutants. 19724625070
lysis of streptococci by an extracellular lysin produced by competent group h streptococcus strain challis. 19724625071
sporulation in bacillus subtilis. characterization of oligosporogenous mutants and comparison of their phenotypes with those of asporogenous mutants. 19724625072
regulation of rna synthesis. 19724625105
studies on the mode of action of nalidixic acid. 19724625117
bacteriophage sp82g inhibition of an intracellular deoxyribonucleic acid inactivation process in bacillus subtilis.the stability of sp82g bacteriophage deoxyribonucleic acid (dna) after its uptake by competent bacillus subtilis was examined by determining the ability of superinfecting phage particles to rescue genetic markers carried by the infective dna. these experiments show that a dna inactivation process within the cell is inhibited after infection of the cell by intact phage particles. the inhibition is maximally expressed 6 min after phage infection and is completely prevented by the addition of chlor ...19724625174
preparation and use of rna-cellulose columns. 19724625254
fractionation of dna from bacillus subtilis and its transforming activity for various markers. 19724625310
dry heat inactivation of bacillus subtilis var. niger spores as a function of relative humidity.dry heat sterilization of bacillus subtilis var. niger spores at 105 c is enhanced in the relative humidity range 0.03 to 0.2%. d-values of 115 and 125 c are predicted by a kinetic model with parameters set from 105 c data. these predictions are compared to observations.19724625341
simple liquid scrubber for large-volume air sampling.a new large-volume air sampler called the "simple liquid scrubber" is described. it can recover a high percentage of microorganisms from large volumes of air, up to 950 liters/min, and concentrate them into a small volume of liquid at a ratio of about 400,000 to 1. the principle of operation of the scrubber is based on the production of a fine mist in a rapidly moving airstream with ultimate collection of the airborne particles by impingement into the film of liquid formed upon impaction of the ...19724625342
scanning electron microscopy of microbial cells on membrane filters.scanning electron micrographs of a pseudomonas species, staphylococcus aureus, and bacillus subtilis on two membrane filtration systems are compared.19724625343
jolipeptin, a new peptide antibiotic. ii. the mode of action of jolipeptin. 19724625359
[thymine requiring strains]. 19724625414
[dna synthesizing mutants of bacillus subtilis]. 19724625415
[new method of phage isolation]. 19724625417
[synchronized cultures]. 19724625418
sterile topical dosage forms. i. laboratory phase. 19724625585
ultraviolet inactivation and excision-repair in bacillus subtilis. 3. sensitized photoinactivation of transforming dna, and the effect of thymine dimers on differential marker inactivation and differential marker repair. 19724625592
ultraviolet inactivation and excision-repair in bacillus subtilis. iv. integration and repair of ultraviolet-inactivated transforming dna. 19724625593
survival of common terrestrial microorganisms under simulated jovian conditions. 19724625765
antimicrobial activity of a series of new 5-nitro-2-furaldehyde aminoacethydrazones. 19724625792
genetic and transfection studies with b. subtilis phage sp50. 3. biological effects of dna cleavage and the physical basis of the map. 19724625810
nalidixic acid does not inhibit bacterial transformation. 19724625811
calcium inhibition of antibacterial activity of kanamycin. 19724625824
water-insolubilisation of glycoside hydrolases with cross-linked poly(acryloylaminoacetaldehyde dimethyl acetal) (enzacryl polyacetal). 19724625874
[antimicrobial activity of 5-oxothiazolidine-2-thione and rhodanine derivatives]. 19724625881
effect of glutaraldehyde on the outer layers of escherichia coli. 19724626088
the use of density gradient centrifugation for the separation of germinated from nongerminated spores. 19724626090
action of supermutagens on the transforming dna of bacillus subtilis. 19724626133
[corneal ulcer and causative organisms]. 19724626203
variability of dna content in individual cells of bacillus. 19724626246
[mutants of bacillus subtilis marburg with late blockage in their sporulation]. 19724626264
in-use contamination of intravenous solutions in flexible plastic containers. 19724626385
Displaying items 3501 - 3600 of 32189