Publications

individual free fatty acids and lactate uptake in the human heart during severe sepsis.	coronary haemodynamics and myocardial metabolism of nonesterified fatty acids (nefa) and lactate were studied in 11 patients with severe sepsis, and compared to 10 control subjects. coronary sinus blood flow was evaluated by thermodilution. arterial and coronary sinus blood samples were collected for the measurement of lactate and total and individual nefa concentrations both in septic and control patients. there was an increase in lactate and total nefa arterial concentrations with a marked inc ...	1988	3232957
an increase of collagen biosynthesis precedes other symptoms of ethanol-induced liver damage in rats.	treatment of rats with 10% ethanol for 6 months induced a 3-4-fold increase of [3h]proline incorporation into liver collagen and enhanced the level of prolyl hydroxylase activity in liver and serum of intoxicated rats. several diagnostic tests used to assess liver disease failed to demonstrate any symptom of liver damage. therefore, increased collagen biosynthesis appears to precede other symptoms of liver damage induced by ethanol.	1988	3234225
[infection by the human immunodeficiency virus and the lymphadenopathy clinical picture. clinico-developmental study of 86 patients].		1988	3247624
[infection by the human immunodeficiency virus and the lymphadenopathy clinical picture. clinico-developmental study of 86 patients].		1988	3247624
[effect of the growth rate on the activity of nitrogen metabolism enzymes in chemostat cultures of candida diddensii and candida tropicalis].		1988	3247869
effects of an extract of feverfew (tanacetum parthenium) on arachidonic acid metabolism in human blood platelets.	it has been suggested that feverfew extracts (fe) inhibit platelet behaviour via effects on platelet sulphydryl (sh) groups. in the present study we found evidence that fe inhibits uptake as well as liberation of arachidonic acid (aa) into/from platelet membrane phospholipids (pl).	1988	3248111
depression of intestinal epithelial cell production rate by hindgut bypass in rats.	influence of hindgut bypass on the epithelial cell production rates of the small and large intestine was tested in rats. hindgut bypass decreased cryptal cell production rates of the jejunum, ileum, cecum, and distal colon by 40-65%. these results suggest that the entrance of ileal effluent into the hindgut and, presumably, the resultant microbial production of short-chain fatty acids are required to maintain the normal epithelial cell proliferation not only in the hindgut but also in the small ...	1988	3249918
a model for simultaneous study of pressure and electric events in the rabbit sphincter of oddi and duodenum.	an animal experimental model featuring simultaneous recordings of electromyography and pressure from the sphincter of oddi (so) and duodenum is demonstrated. on the basis of data from 10 rabbits, pressure recordings from the so were shown to have phasic activity with an average basic pressure peak interval of 2.6 to 3.5 sec. histographic analysis of so pressure recordings showed a multimodal appearance, suggesting that the activity is paced. a substantial amount of overlap between so and duodena ...	1988	3249919
significance of heel pad confinement for the shock absorption at heel strike.	shock absorption (sa) is a simple way to reduce the body load and can be used in the prevention and treatment of injuries. the heel pad is the most important shock absorber in the shoe heel complex. the purpose of this study was to investigate whether the sa at heel strike can be increased by heel support in people and shoes with high or low sa. the impact forces at heel strike were measured on an amti (r) force platform. fourteen legs were tested in seven persons (nine with normal and five with ...	1988	3253241
significance of heel pad confinement for the shock absorption at heel strike.	shock absorption (sa) is a simple way to reduce the body load and can be used in the prevention and treatment of injuries. the heel pad is the most important shock absorber in the shoe heel complex. the purpose of this study was to investigate whether the sa at heel strike can be increased by heel support in people and shoes with high or low sa. the impact forces at heel strike were measured on an amti (r) force platform. fourteen legs were tested in seven persons (nine with normal and five with ...	1988	3253241
phagocytic and bactericidal properties of macrophages of mice immunized with outer membrane proteins (omp) of shigella.	macrophages obtained from peritoneal exudates of mice immunized with the single doses (1 and 5 micrograms) of omp were shown to have stronger phagocytic as well as bactericidal properties in relation to shigella flexneri bacilli than nonactivated macrophages. macrophages from the animals immunized with 10, 20 and 30 micrograms omp doses showed phagocytic and bactericidal properties similar to those of nonactivated macrophages while the immunization with the dose 50 micrograms resulted in their s ...	1988	3254119
the role of serum factors and influence of intravenous immunoglobulins on phagocytosis of campylobacter species.		1988	3255396
the relationship between blood and fertility parameters in post partum dairy cows.		1988	3256237
the relationship between blood and fertility parameters in post partum dairy cows.		1988	3256237
the effects of anions on fluid reabsorption from the proximal convoluted tubule of the rat.	1. fluid reabsorption from surface proximal tubules of the rat was measured in vivo using stationary microperfusion techniques. reabsorptive rate (jv) was measured from droplets containing chloride as the main reabsorbable anion and when chloride was substituted by bromide, iodide, nitrate, acetate, isethionate or methylsulphate in either the tubular lumen alone or in both lumen and peritubular capillaries. 2. in tubules with an intact blood supply, droplet volume decreased in a manner best desc ...	1988	3256612
reticuloendothelial clearance and splenic mononuclear cell populations after resection and autotransplantation.	although the preservation of splenic tissue may prevent overwhelming infection after splenectomy, the degree of protection conferred by small remnants has not been optimal. we investigated whether either splenic reticuloendothelial clearance of a blood flow-dependent colloid or macrophage and t-cell populations might be altered by resection or autotransplantation of the spleen. our results have shown that bloodstream reticuloendothelial clearance of technetium 99m sulfur colloid is not impaired ...	1988	3257658
hydrogen peroxide-mediated inhibition of t-cell response to mitogens is a result of direct action on t cells.	hydrogen peroxide, a reactive oxygen intermediate produced by activated neutrophils, has been shown to inhibit the response of human t lymphocytes to mitogens and alloantigens. since hydrogen peroxide is known to react with iron and to induce lipid peroxidation, we compared the effects of hydrogen peroxide and a lipid peroxidation product, malondialdehyde, on the response of human peripheral blood mononuclear cells to t-cell mitogens. peripheral blood mononuclear cells pretreated with 1 mmol/l o ...	1988	3257684
lymphocyte chimerism in a full allogeneic composite tissue (rat-limb) allograft model prolonged with cyclosporine.	lew recipients of aci vascularized hind limb allografts were analyzed for lymphoid chimerism by a complement-dependent cytotoxicity assay using antisera produced across this strain combination. in assessing the technique, two lew recipients of sublethal irradiation (400 rad), aci bone-marrow allografts, and csa exhibited mixed lymphoid chimerism 23 days posttransplant. short-term csa-treated cta recipients that were assayed at various times following transplantation and underwent subacute reject ...	1988	3259042
human cryptosporidiosis in north queensland.	during a 12-month period, feces from 780 persons from the townsville region were evaluated by the kinyoun acid-fast strain, and 36 (4.6%) immunocompetent patients were found to have cryptosporidium oocysts. twenty-five index cases were identified; 13 (8.6%) cases from 151 patients were from palm island, an isolated aboriginal community in the wet tropics and 12 (1.9%) cases from 629 patients were from the dry tropics of townsville. all 11 secondary cases were associated with a person-to-person o ...	1988	3264149
electrophysiologic effects of aspirin on myocardium: interaction with catecholamine.		1988	3269682
electrophysiologic effects of aspirin on myocardium: interaction with catecholamine.		1988	3269682
contamination of freshly slaughtered pig carcasses with human pathogenic yersinia enterocolitica.	evidence is presented for the extent of contamination of freshly slaughtered pig carscasses with human pathogenic yersinia enterocolitica and shows the significance of faecal contamination as a source of infection. swab samples collected from the rectum and the surface of a total of 1458 pig carcasses were examined for the presence of human pathogenic y. enterocolitica. y. enterocolitica, biovar iv, serogroup 0:3, were isolated from the rectum of 360 pigs (24.7%). the organism was isolated from ...	1988	3275322
experimental portal fibrosis produced by intraportal injection of killed nonpathogenic escherichia coli in rabbits.	an attempt was made to develop an animal model for the study of the etiology of noncirrhotic portal fibrosis or idiopathic portal hypertension based on the assumption that it is related to chronic abdominal infection. rabbits were given killed nonpathogenic escherichia coli intraportally or intravenously. the animals to which a mixture of killed e. coli and rabbit antiserum (aggregated e. coli) was given intraportally developed remarkable histologic changes in the liver. the early inflammatory r ...	1988	3276575
shigella bacteremia.		1988	3277577
a basic replicon of virulence-associated plasmids of shigella spp. and enteroinvasive escherichia coli is homologous with a basic replicon in plasmids of incf groups.	shigella species and enteroinvasive escherichia coli strains carry a large (120- to 140-megadalton) plasmid called pinv, which contains genes essential for the invasiveness of these pathogens. hybridization with specific probes derived from the repfic and repfib replicons of the incf1 ent plasmid p307 showed that pinvs present in 35 clinical isolates are homologous with repfic but not repfib, regardless of the serogroup of the shigella or e. coli strain. repfic of p307, in turn, is very similar ...	1988	3278983
combined parenteral and oral immunization results in an enhanced mucosal immunoglobulin a response to shigella flexneri.	achieving a vigorous secretory immunoglobulin a (iga) response in intestinal secretions usually requires multiple doses of antigen given orally, while systemic immunity is more easily attained by parenteral immunization. this study examines the role of combined parenteral and oral immunizations to enhance the early mucosal immune response to an enteropathogen. we have used a chronically isolated intestinal-loop model in rabbits as a probe to monitor kinetically the initial (primary) local immune ...	1988	3278985
protein binding sites on escherichia coli 16s ribosomal rna; rna regions that are protected by proteins s7, s9 and s19, and by proteins s8, s15 and s17.	selected groups of isolated 14c-labelled proteins from e. coli 30s ribosomal subunits were reconstituted with 32p-labelled 16s rna, and the reconstituted complexes were partially digested with ribonuclease a. rna fragments protected by the proteins were separated by gel electrophoresis and subjected to sequence analysis. complexes containing proteins s7 and s19 protected an rna region comprising helices 29 to 32, part of helix 41, and helices 42 and 43 of the 16s rna secondary structure. additio ...	1988	3279390
toxoplasmosis and the acquired immune deficiency syndrome.	the literature on toxoplasmosis in the acquired immune deficiency syndrome is reviewed with reference to more than 140 cases from various centres. the incidence, pathogenesis, clinical features, diagnosis, treatment and prognosis are considered.	1988	3280690
quality of commercially produced shigella serogrouping and serotyping antisera.	shigella grouping antisera from five manufacturers and typing antisera from two were purchased and evaluated with homologous and heterologous shigella strains in the slide agglutination test. only 31 of 73 (42%) antisera were satisfactory. in many instances, the antisera gave negative, as opposed to weak, reactions when they should have given strong positive reactions. four reagents cross-reacted with shigella strains. of the 19 polyvalent grouping antisera to subgroups shigella dysenteriae sero ...	1988	3281972
cardiac nuclear medicine, present status, future hopes as seen from the view of a cardiologist.	the present techniques of nuclear medicine that are used in cardiology are critically evaluated. they are used mainly for the assessment of coronary circulation (measurement of regional flow and perfusion), on the determination of left ventricular muscle function (radionuclear ventriculography) and on the study of cardiac metabolism. the last-mentioned technique is unique insofar as (except for magnetic resonance imaging) there is no other method available to analyse myocardial metabolism noninv ...	1988	3282883
reaction of anti-hla-b monoclonal antibodies with envelope proteins of shigella species. evidence for molecular mimicry in the spondyloarthropathies.	immunologic cross-reactivity between enteric bacteria and the hla-b27 protein may play a role in the etiology of reiter's syndrome and reactive arthritis. the reactivity of two anti-b27 mab (b27m1 and b27m2) with envelope proteins of shigella flexneri isolated from reiter's syndrome patients was studied by western blot analysis. proteins with an apparent mr of approximately 36 and 23 kda reacted with both mab in ascites. mab against related hla class i ag b7 and b40 did not react with the 23 kda ...	1988	3283236
serum antibodies to lipopolysaccharide and natural immunity to shigellosis in an israeli military population.		1988	3283258
conservation of an atp-binding domain among reca proteins from proteus vulgaris, erwinia carotovora, shigella flexneri, and escherichia coli k-12 and b/r.	the purified reca proteins encoded by the cloned genes from proteus vulgaris, erwinia carotovora, shigella flexneri, and escherichia coli b/r were compared with the reca protein from e. coli k-12. each of the proteins hydrolyzed atp in the presence of single-stranded dna, and each was covalently modified with the photoaffinity atp analog 8-azidoadenosine 5'-triphosphate (8n3atp). two-dimensional tryptic maps of the four heterologous reca proteins demonstrated considerable structural conservation ...	1988	3286605
[clinico-immunological characteristics of mixed intestinal infections in children].		1988	3287323
[changes in the degree of sensitivity to antibiotics of shigella isolated from patients with dysentery in a city infectious disease hospital over the past 8 years].	changes in sensitivity of shigella and in particular shigella flexneri isolated from dysentery patients in a city infection hospital within 8 years i.e. from 1977 to 1985 were followed. it was shown that the changes were to the greater extent connected with preferable use of certain antibiotics in the hospital.	1988	3288150
suggestion to supplement shigella flexneri classification scheme with the subserovar shigella flexneri 4c: phenotypic characteristics of strains.	a new serovar of shigella flexneri has been isolated with increasing frequency in the ussr. it has the antigenic formula iv:7,8. we suggest that it be incorporated into the classification scheme as s. flexneri subserovar 4c. the 212 strains of this proposed subserovar examined to date display biochemical and serological properties typical of the species, are sensitive to the genus-specific bacteriophage, and cause keratoconjunctivitis in the sereny test.	1988	3290245
salmonellal mycotic abdominal-aortic aneurysm.	a patient is described with a mycotic abdominal-aortic aneurysm that was caused by salmonella typhimurium, who presented to hospital four months after a urinary-tract infection that was caused by the same organism. the urinary-tract infection may have resulted from an episode of transient salmonellal bacteraemia, the significance of which was not appreciated. the pathogenesis, clinical features, treatment and prognosis of arteritis due to salmonella species are reviewed. the early diagnosis and ...	1988	3292882
salmonellal mycotic abdominal-aortic aneurysm.	a patient is described with a mycotic abdominal-aortic aneurysm that was caused by salmonella typhimurium, who presented to hospital four months after a urinary-tract infection that was caused by the same organism. the urinary-tract infection may have resulted from an episode of transient salmonellal bacteraemia, the significance of which was not appreciated. the pathogenesis, clinical features, treatment and prognosis of arteritis due to salmonella species are reviewed. the early diagnosis and ...	1988	3292882
demetrio sodi-pallares: the man and his thought.		1988	3293862
identification of a unique lymphocyte subpopulation in the sheep uterus.	a panel of monoclonal antibodies was used to define the lymphocyte subpopulations in the sheep uterus at various stages of the oestrous cycle. a striking finding was that the majority of lymphocytes in the uterine and endometrial glandular epithelia belonged to a unique lymphocyte subpopulation that expressed the cd45r antigen but was negative for major histocompatibility complex (mhc) class ii molecules and expressed low or undetectable levels of the cd5 antigen. when examined under the electro ...	1988	2962930
relationship of free intracellular calcium to the cytolytic activity of entamoeba histolytica.	entamoeba histolytica adherence and destruction of host cells is required for in vivo pathogenicity; amebic in vitro adherence is mediated by a galactose- or n-acetyl-d-galactosamine-inhibitable surface lectin (gal/galnac adherence lectin). free intracellular ca2+ concentration [( ca2+]i) was measured in living amebae and target cells during amebic cytolysis of chinese hamster ovary (cho) cells and human polymorphonuclear neutrophils by utilizing the ca2+ probe fura-2 and computer-enhanced digit ...	1988	2897335
antimicrobial susceptibility of bordetella pertussis (part i).	in this review of the literature data are collected from the more recent studies on the susceptibility of bordetella pertussis to penicillins, cephalosporins, other beta-lactam antibiotics, aminoglycosides, tetracyclines, erythromycin, josamycin, co-trimoxazole and various other antibiotics. the methods of susceptibility testing of b. pertussis are discussed and suggestions for standardization are made.	1988	2897337
the high molecular weight receptor to transforming growth factor-beta contains glycosaminoglycan chains.	proteoglycans are constituents of the cell surface that may play important roles in the regulation of cell behavior. here we report that the 250-kda receptor subunit that binds the multifunctional protein, transforming growth factor-beta 1 (tgf-beta 1), contains chains of heparan sulfate and chondroitin sulfate and thus is a proteoglycan. digestion of tgf-beta 1-receptor complexes with glycosaminoglycan (gag)-specific degradative enzymes yield core proteins of 115-140 kda. cell monolayers that h ...	1988	2897367
a selective inhibitor of camp-specific phosphodiesterase, ro 20-1724, has no effect on the quantal release of acetylcholine from the mouse phrenic nerve.	the indirect twitch response of the mouse isolated phrenic nerve-diaphragm preparation, partially paralysed by tubocurarine, was restored only by about 10% by ro 20-1724 at 2 to 280 microm. the solvent vehicle, dimethylsulphoxide, also showed the same effect to a similar extent. intracellular recordings with glass microelectrodes revealed that ro 20-1724 (40 microm) affected neither the resting membrane potential, the amplitude and frequency of miniature endplate potentials nor the amplitude of ...	1988	2897454
apa i and sst i rflps at the insulin-like growth factor ii (igf2) locus on chromosome 11.		1988	2897668
human herpesvirus 6 and the henle-koch postulates.		1988	2901654
interaction between anti-influenza viral polymerase antibodies and rnp particles using the in vitro transcription process and an immunogold labelling technique.	immunogold labelling and in vitro transcription of influenza virus vrna have been used to analyse the interaction of anti-influenza polymerase antibodies with influenza-ribonucleoprotein (rnp) complexes. the polymerase proteins (p proteins) were localized exclusively at one end of the rnp segments. in the course of transcription the amount of p protein decreased significantly. the in vitro transcriptase activity y of influenza a virus rnp complexes in the presence of anti-polymerase antibodies t ...	1988	2904734
blockade of spinal pathways decreases pre- and postganglionic discharge differentially.	although discharge of many sympathetic nerves decreases substantially on transection of the spinal cord in cats, firing of splenic and mesenteric postganglionic nerves continues unabated (r. l. meckler and l. c. weaver, brain res. 338: 123-135, 1985; r. d. stein and l. c. weaver. j. physiol. lond. 396: 155-172, 1988). therefore, ongoing sympathetic outflow directed to the splanchnic circulation was proposed to be less dependent on supraspinal excitatory drive than that directed to other vascular ...	1988	2904774
pharmacological properties of the positive inotropic and alpha 1-adrenoceptor blocking agent saterinone.	the pharmacological properties of saterinone [+/-)-1,2-dihydro-5-[4-[2-hydroxy-3-[4-(2-methoxyphenyl)-1-piperazinyl] propoxy]phenyl]-6-methyl-2-oxo-3-pyridine-carbonitrile, bdf 8634) were investigated in isolated organs of the guinea pig and in human platelets. saterinone was found to be a potent antagonist at vascular alpha 1-adrenoceptors with a pa2-value of 8.46 +/- 0.12. besides its affinity for alpha 1-adrenoceptors saterinone exerted a positive inotropic effect in the isolated papillary mu ...	1988	2906245
[effects of prazosin, sgb-1534, dobutamine and isoproterenol on congestive heart failure in dogs].	effects of prazosin, dobutamine, isoproterenol and sgb-1534, a new alpha-blockade, on congestive heart failure (chf) in dogs were investigated. the model was made by protease injection into the left ventricular free wall, saline loading, and dextran and methoxamine infusions. by this maneuver, left atrial pressure (lap), systemic vascular resistance (svr) and left ventricular end-diastolic pressure (lvedp) were markedly increased, aortic blood flow (aobf) was decreased, and systemic blood pressu ...	1988	2906315
malaria as anthropo-ecosystem. part i: general concept.		1988	2906647
the epidemiology of s. sonnei and s. flexneri in pima county, arizona: an exploratory study.	investigation of 189 cases of shigellosis reported to the pima county, arizona health department in 1986 revealed that 23 per cent of cases could be attributed to travel to mexico, and 10 per cent to day care attendees and their household contacts. no source of infection or high-risk activity could be demonstrated for 43 per cent of the cases. households in which s. flexneri occurred were more likely to be characterized by crowded living situations and to have no known source of infection.	1988	3044149
isolation of shiga toxin-resistant vero cells and their use for easy identification of the toxin.	shiga toxin-resistant vero cells were isolated by treatment of the cells with nitrosoguanidine. these mutant cells were not affected by shiga toxin at more than 1 microgram/ml, although the parent vero cells were sensitive to 25 pg of the toxin per ml. immunofluorescence studies showed that all the mutant cells had lost toxin-binding capacity. the cytotoxic activities of various bacterial cultures against the parent and mutant cells were compared. all samples from 10 strains of shigella dysenter ...	1988	3045003
congo red binding and salt aggregation as indicators of virulence in shigella species.	smooth strains of shigella dysenteriae type 1, shigella flexneri, shigella boydii, and shigella sonnei which form pigmented colonies (pcr+) on congo red agar were virulent in the sereny test. smooth variants unable to bind congo red (pcr-) were avirulent. measurements of dye uptake from solution showed that s. dysenteriae type 1 bound the most dye, followed in order of uptake by s. flexneri, s. boydii, and s. sonnei. using the salt aggregation test (sat) to determine cell surface hydrophobicity, ...	1988	3045151
clinical and microbiologic features of shigella and enteroinvasive escherichia coli infections detected by dna hybridization.	to determine the clinical and microbiologic features of shigella and enteroinvasive escherichia coli (eiec) infections, we investigated 410 children with diarrhea and 410 control children without diarrhea who were seen at children's hospital, bangkok, thailand, from january to june 1985. shigella spp. were isolated from 96 (23%) and eiec were isolated from 17 (4%) of 410 children with diarrhea and from 12 (3%) and 6 (1%) of 410 control children, respectively. the isolation rates of both pathogen ...	1988	3045152
glycocalyx in virulent and avirulent strains of shigella flexneri.		1988	3045162
[morphologic manifestations of the medicinal lysis of a coronary artery thrombus].	morphologic studies of coronary arterial thrombi have shown immature fibrin fibres to prevail in those, with fibrin degradation products always present. this fact suggests that fibrinolytic components of hemostasis, limiting the rates of fibrin polymerization and maturation, may participate in thrombogenesis. at the same time, macrophages that phagocytose fibrin and blood cells (platelets and erythrocytes) become activated since the early hours of the thrombus' existence. selective administratio ...	1988	3045402
[mechanism of the nonspecific protective action of shigella antigens].	the mechanism promoting the nonspecific action of antigens obtained from s. flexneri and s. sonnei by a sparing method has been studied. these antigens stimulate the t- and b- systems of immunity, that is followed by activation of myelopoiesis and the humoral protective factors of the body, which seems to underlie the formation of resistance to infection caused by nonspecific microorganisms.	1988	3046195
immunoreactivity of a surface wall fraction produced by spherules of coccidioides immitis.	the membranous spherule outer wall (sow) isolated from liquid cultures of coccidioides immitis has been shown to elicit reactivity with human anti-coccidioides antibody by immunofluorescence and the immunodiffusion-tube precipitin assay. the serologically reactive components were extracted from sow with the nonionic detergent n-octyl-beta-d-glucopyranoside (og). the og-soluble fraction of sow was shown to be reactive with immunoglobulin g in 25 serum samples from coccidioidomycosis patients by a ...	1988	3047063
immunoreactivity of a surface wall fraction produced by spherules of coccidioides immitis.	the membranous spherule outer wall (sow) isolated from liquid cultures of coccidioides immitis has been shown to elicit reactivity with human anti-coccidioides antibody by immunofluorescence and the immunodiffusion-tube precipitin assay. the serologically reactive components were extracted from sow with the nonionic detergent n-octyl-beta-d-glucopyranoside (og). the og-soluble fraction of sow was shown to be reactive with immunoglobulin g in 25 serum samples from coccidioidomycosis patients by a ...	1988	3047063
[lipid peroxidation in the pathogenetic and clinical aspects of infectious diseases].		1988	3047989
blood-injury phobia: a review.	natural human uneasiness about blood, injury, or deformity sometimes becomes a specific phobia, which can lead to serious disability if vital medical procedures are refused. blood-injury phobia usually starts in childhood and is often familial. unlike other phobic cues, which cause persistent tachycardia, blood-injury phobic cues evoke an initial rise in heart rate followed by vasovagal bradycardia and, frequently, syncope. although blood-injury phobia may have an evolutionary, genetic, and phys ...	1988	3048117
deletion of the shiga toxin gene in a chlorate-resistant derivative of shigella dysenteriae type 1 that retains virulence.	we used a probe specific for detecting the structural-gene sequences of shiga toxin to analyze the genetic nature of toxin synthesis in mutant derivatives of shigella dysenteriae type 1. a chlorate-resistant (chl) mutant (725-78) of s. dysenteriae type 1 strain 3818t, which had retained virulence but had lost production of high levels of cytotoxic activity associated with shiga toxin synthesis, contained a complete deletion of the shiga toxin structural-gene sequences. these structural-gene sequ ...	1988	3049838
[pro-allergy role of infection. a component of the mode of reacting].	no organism from the cradle lives germ-free or allergen-free. the modalities by which infections facilitate conditions of abnormal reactivity, in particular respiratory asthma and allergy to cow milk proteins, are examined. 216 asthmatic children and 50 infants with rotavirus enteritis have been considered. infections, besides representing stimuli directly projected on the immune system, also constitute factors which more generally influence the way of reacting.	1988	3050902
[differentiation of virulent strains of shigella and entero-invasive escherichia from their avirulent variants using modified indirect immunoenzyme analysis].	the data obtained in this investigation confirm that the modified indirect enzyme immunoassay (eia) permits the differentiation of virulent bacteria of the genus shigella and enteroinvasive escherichia (group 1), regularly containing virulence plasmids with a molecular weight of 120-140 md, from their avirulent variants which have lost these plasmids (group 2). the ratio of the optic density (od) values of the positive control samples (the od of group 1) to the od values of the negative ones (th ...	1988	3051808
the persistence of shigella flexneri in the united states: increasing role of adult males.	the annual reported isolation rate of shigella flexneri decreased from 1964 to 1973, but has remained constant since then at 1 per 100,000. between 1975 and 1985, the median age of males from whom s. flexneri was isolated rose from 5 to 26 years. during this time, the isolation rate of s. flexneri rose more than five-fold among men, did not change in adult women, and decreased in children. by 1985, 23 per cent of reported s. flexneri isolates came from men aged 20-49. increased male homosexual t ...	1988	3052115
the epidemiology of shigellosis in israel.	analysis of shigella isolates referred for serotyping to the central shigella reference laboratory has revealed a slight predominance of s. sonnei (47%) over s. flexneri (44%) in israel. in female patients, the predominance of s. sonnei isolates was more accentuated (54 v. 37.5% for s. flexneri). on the other hand, an increased incidence of s. flexneri was observed in military personnel (59 v. 32% for s. sonnei), and even more so in the arab population (69 v. 19%). incidence peaked in summer (ju ...	1988	3052334
the ecology and pathogenicity of urease-producing bacteria in the urinary tract.	urease activity is a physiological function of many bacteria that enables these organisms to utilize urea as a source of nitrogen. the association of ureolytic bacteria with human or animal hosts varies widely from a commensal relationship as demonstrated with skin microflora, a symbiotic relationship in the gastrointestinal tract, to a pathogenic relationship in the urinary tract. since similar or identical species of bacteria such as staphylococcus aureus are found in all three environments, t ...	1988	3053050
the ecology and pathogenicity of urease-producing bacteria in the urinary tract.	urease activity is a physiological function of many bacteria that enables these organisms to utilize urea as a source of nitrogen. the association of ureolytic bacteria with human or animal hosts varies widely from a commensal relationship as demonstrated with skin microflora, a symbiotic relationship in the gastrointestinal tract, to a pathogenic relationship in the urinary tract. since similar or identical species of bacteria such as staphylococcus aureus are found in all three environments, t ...	1988	3053050
shigella flexneri bacteraemia in post partum period.		1988	3053638
[a case using plasmapheresis in treating infectious toxicosis in young infants].		1988	3054796
[stability of shigella flexneri attenuated strain 2a 77 ade- rif-r after cultivation in a fermenter and lyophilization].	the method for the cultivation of s. flexneri attenuated strain 2a 77 ade- rifr in a fermenter with the use of casein fermentative broth containing 200-250 mg% of amino nitrogen has been developed. no changes in the properties of the initial strain have been found after its cultivation in a fermenter or after the lyophilization of the cultures grown in a fermenter. after cultivation in casein fermentative broth with 100 mg% of amino nitrogen the presence of s-r dissociation has been established, ...	1988	3055763
characterization of invasion plasmid antigen genes (ipabcd) from shigella flexneri.	the large invasion plasmid of shigella flexneri m9ot-w was used to generate recombinant plasmids carrying the ipaa, -b, -c, and -d genes, whose products are associated with the entry of the bacteria into colonic epithelial cells. complete dna sequences of ipab, -c, and -d were determined. the proteins predicted (62, 42, and 37 kda, respectively) from the nucleotide sequences lack a signal-peptide sequence. hydrophilic segments of the ipab and ipac proteins were found to overlap known epitopic do ...	1988	3057506
persistent shigellosis.	shigella usually causes a self-limited infection which untreated lasts on average seven days (range one to 30 days). we report a patient who had persistent symptoms for 10 months caused by shigella flexneri which was finally identified on cultures from a rectal swab.	1988	3058558
affinity chromatography of reca protein and reca nucleoprotein complexes on reca protein-agarose columns.	we have analyzed the nature of reca protein-reca protein interactions using an affinity column prepared by coupling reca protein to an agarose support. when radiolabeled soluble proteins from escherichia coli are applied to this column, only the labeled reca protein from the extract was selectively retained and bound tightly to the affinity column. efficient binding of purified 35s-labeled reca protein required mg2+, and high salt did not interfere with the binding of reca protein to the column. ...	1988	3058701
[etiologic structure of bacterial dysentery in the ussr in 1983-1985].	the etiological structure of dysentery in the ussr in 1983-1985 is characterized. sonne dysentery was found to prevail in the territories with adequate water supply, while dysentery caused by shigella flexneri prevailed at the territories with unsatisfactory water supply. s. dysenteriae and s. boydii were found to play a limited role in the etiology of dysentery. in the presence of global pandemic, an increase in the isolation rate of s. dysenteriae i in the ussr is observed. the data on the bio ...	1988	3061262
a 13c-n.m.r. study of some shigella flexneri o-polysaccharides.	13c-n.m.r. data for some shigella flexneri o-polysaccharides are reported. it is concluded that the chemical shifts observed for the linear shigella flexneri type y o-polysaccharide are similar to those calculated by adding substituent shifts, obtained from disaccharides with similar stereochemistry around the glycosidic linkage, to the chemical shifts for the monomers. the influence of substitution with alpha-d-glucopyranosyl and/or o-acetyl groups in different positions of this polysaccharide ...	1988	3061647
[pathogenetic significance of virulence-associated genes in shigella flexneri].		1988	3063851
interaction between vestibulocollic and cervicocollic reflexes: automatic compensation of reflex gain by muscle afferents.		1988	3064144
[electron microscopic study of the interaction of virulent and avirulent shigella flexneri strains with the macrophages of murine peritoneal exudate].	the electron microscopic dynamic study of the interaction of s. flexneri virulent strain 2a and s. flexneri avirulent strain, genetically related to strain 2a and having the same antigenic structure, with peritoneal macrophages of cba mice at an early period showed differences in the cytotoxic action of these strains. even at the early period of the interaction between macrophages and virulent shigellae structural changes were observed in macrophages: ruptures in the membranes of parasitophore p ...	1988	3064506
[scientific bases of epidemiologic surveillance of intestinal infections].		1988	3064508
arginyl-trna synthetase from escherichia coli, purification by affinity chromatography, properties, and steady-state kinetics.	a blue sephadex g-150 affinity column adsorbs the arginyl-trna synthetase of escherichia coli k12 and purifies it with high efficiency. the relatively low enzyme content was conveniently purified by deae-cellulose chromatography, affinity chromatography, and fast protein liquid chromatography to a preparation with high activity capable of catalyzing the esterification of about 23,000 nmol of arginine to the cognate trna per milligram of enzyme within 1 min, at 37 degrees c, ph 7.4. the turnover ...	1988	3064807
arginyl-trna synthetase from escherichia coli, purification by affinity chromatography, properties, and steady-state kinetics.	a blue sephadex g-150 affinity column adsorbs the arginyl-trna synthetase of escherichia coli k12 and purifies it with high efficiency. the relatively low enzyme content was conveniently purified by deae-cellulose chromatography, affinity chromatography, and fast protein liquid chromatography to a preparation with high activity capable of catalyzing the esterification of about 23,000 nmol of arginine to the cognate trna per milligram of enzyme within 1 min, at 37 degrees c, ph 7.4. the turnover ...	1988	3064807
serological response to plasmid-encoded antigens in children and adults with shigellosis.	the immunological response to plasmid-encoded antigens of virulent shigella was determined in thai children less than 4 yr of age and in thai adults by immunoblot analysis and elisa. forty-two percent (8/19) of thai children and 4% (1/22) of thai adults with shigellosis developed a greater than or equal to 4-fold rise in igg antibody titer to water-extracted antigens of shigella flexneri m90t by elisa (p = 0.006). two children and one lactating mother with shigellosis developed a 4-fold rise in ...	1988	3066570
regulation of urea cycle enzymes.		1988	3067149
[characteristics of the course of acute dysentery in a 5-year-old child complicated 3 times by intestinal invagination].		1988	3067205
physiological and behavioral aspects of stress in adolescence.	gonadal and adrenal hormone levels appear to be linked to the stressors experienced by young adolescents. adjustment problems were accompanied by a profile of lower gonadal steroids and higher adrenal androgen levels, primarily androstenedione. later gonadal maturation may be a result of stress suppressing the reproductive axis. higher levels of androstenedione may be indicative of chronic levels of stress. however, the findings for androstenedione are complicated by the fact that androstenedion ...	1988	3067560
genetic relatedness of the basic replicon of the virulence plasmid in shigellae and enteroinvasive escherichia coli.	the regions required for replication (rep) and for stable maintenance (stb) of the 230 kilobase virulence plasmid, pmysh6000, in shigella flexneri 2a ysh6000 were identified on the restriction enzyme sa/l fragments, c and o, respectively. the stable plasmid, pmysh6610, consisting of adjacent fragments c and o was found to belong to the incompatibility group fl. in all strains of shigellae and enteroinvasive escherichia coli (eiec) tested, virulence plasmids were found to be highly homologous wit ...	1988	3070262
[vitamin d as an immuno-hematopoietic hormone. new perspectives on a previously known substance].	1,25-dihydroxyvitamin d3 (1.25[oh]2d3), the most active metabolite of vitamin d3, plays an important role in bone and mineral metabolism. there is growing evidence that the vitamin d endocrine system may be important in other systems as well: 1,25(oh)2d3 is synthesized in extrarenal tissue. macrophages from both normal human individuals and patients with sarcoidosis are capable of producing 1,25(oh)2d3. specific receptors for 1,25(oh)2d3 are present in a number of cells and tissues other than th ...	1988	3070739
[the importance of secondary immune deficiencies in the pathogenesis of dysentery].	some immunity indices were studied in patients with acute dysentery. the number of total and active t-lymphocytes, t mu- and t gamma-cells, t-rabbit, t-staphylococcal, b-total and b-lymphocytes forming rosettes with mouse erythrocytes were determined for assessment of the immune status. changes in the above indices with relation to the severity of the disease were noted. suppression of the t- and b-systems of immunity were noticeable in patients with a severe and lingering course of disease. del ...	1988	3070808
[free-radical oxidation of blood plasma lipids in patients with acute intestinal infections].	blood plasma free radical lipid oxidation was studied in 98 patients with acute dysentery (ad) and 64 patients with food toxinfection (fti). free radical lipid oxidation was assessed by the intensity of chemiluminescence and the level of lipid peroxides. activation of blood plasma lipid peroxidation was established with relation to a clinical variant of the disease. the effect of indomethacin on the parameters of free radical lipid oxidation was noted in patients with ad and fti.	1988	3070810
nucleotide sequence of the invasion plasmid antigen b and c genes (ipab and ipac) of shigella flexneri.	the nucleotide sequence of a 4.8 kilobase (kb) hindiii fragment from pwr100, the virulence plasmid of shigella flexneri 5, was determined and analysed. this fragment encodes polypeptides b (62 kilodalton, kd) and c (43 kd) which have already been described as two of the four immunogenic polypeptides of shigellae. the nucleotide sequence revealed that in addition to the ipab and ipac genes encoding polypeptides b and c, a third complete open reading frame was found within the fragment. the gene, ...	1988	3071655
chronic shigella infection in an hiv seropositive patient--ontario.		1988	3072101
outbreak of shigella flexneri in a nursing home--alberta.		1988	3072103
plasmid mediated drug resistance in shigella flexneri serotypes 1-6 during 1974-85.		1988	3072296
[an analysis of distribution of shigella groups for thirty-seven years (1949-1985) in china].		1988	3073017
[evaluation of selective properties of mac conkey's medium enriched with potassium tellurite for growing shigella and yersinia strains].		1988	3073281
shigellemia in an infant with intractable diarrhea.		1988	3074059
laboratory studies on the mode of action and application of antiestrogen for breast cancer therapy.		1988	3074131
laboratory studies on the mode of action and application of antiestrogen for breast cancer therapy.		1988	3074131