Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| reduced catabolic protein expression in clostridium butyricum dsm 10702 correlate with reduced 1,3-propanediol synthesis at high glycerol loading. | higher initial glycerol loadings (620 mm) have a negative effect on growth and 1,3-propanediol (1,3-pdo) synthesis in clostridium butyricum dsm 10702 relative to lower initial glycerol concentrations (170 mm). to help understand metabolic shifts associated with elevated glycerol, protein expression levels were quantified by lc/ms/ms analyses. thirty one (31) proteins involved in conversion of glycerol to 1,3-pdo and other by-products were analyzed by multiple reaction monitoring (mrm). the analy ... | 2014 | 25401066 |
| evaluation of a modified cefsulodin-irgasan-novobiocin agar for isolation of yersinia spp. | y. enterocolitica and y. pseudotuberculosis are important food borne pathogens. however, the presence of competitive microbiota makes the isolation of y. enterocolitica and y. pseudotuberculosis from naturally contaminated foods difficult. we attempted to evaluate the performance of a modified cefsulodin-irgasan-novobiocin (cin) agar in the differentiation of y. enterocolitica from non-yersinia species, particularly the natural intestinal microbiota. the modified cin enabled the growth of y. ent ... | 2014 | 25170941 |
| prevalence and impact of extended-spectrum β-lactamase production on clinical outcomes in cancer patients with enterobacter species bacteremia. | we examined the prevalence of extended-spectrum β-lactamase (esbl) production and the impact of esbl on clinical outcomes in cancer patients with enterobacter spp. bacteremia. | 2014 | 25228840 |
| low-shear force associated with modeled microgravity and spaceflight does not similarly impact the virulence of notable bacterial pathogens. | as their environments change, microbes experience various threats and stressors, and in the hypercompetitive microbial world, dynamism and the ability to rapidly respond to such changes allow microbes to outcompete their nutrient-seeking neighbors. viewed in that light, the very difference between microbial life and death depends on effective stress response mechanisms. in addition to the more commonly studied temperature, nutritional, and chemical stressors, research has begun to characterize m ... | 2014 | 25149449 |
| a retrospective review of the clinical experience of linezolid with or without rifampicin in prosthetic joint infections treated with debridement and implant retention. | debridement and prosthesis retention, combined with a prolonged antibiotic regimen including rifampicin, is an accepted therapeutic approach when the duration of symptoms is less than 4 weeks and there are no radiological signs of loosening. the outcome of patients managed with this strategy has been previously assessed in several articles with success rates of 60-90%. this study aims to review the clinical experience with linezolid in 3 different hospitals from spain and france in patients with ... | 2014 | 25139552 |
| light scattering sensor for direct identification of colonies of escherichia coli serogroups o26, o45, o103, o111, o121, o145 and o157. | shiga-toxin producing escherichia coli (stec) have emerged as important foodborne pathogens, among which seven serogroups (o26, o45, o103, o111, o121, o145, o157) are most frequently implicated in human infection. the aim was to determine if a light scattering sensor can be used to rapidly identify the colonies of stec serogroups on selective agar plates. | 2014 | 25136836 |
| high isolation rates of multidrug-resistant bacteria from water and carpets of mosques. | there is little information regarding the isolation of antimicrobial-resistant potentially pathogenic bacteria from water and carpets of mosques worldwide. the objective of the present investigation is to determine the bacteriological quality of water and carpets of mosques in elkhomes city in libya. | 2014 | 25128691 |
| type ii and type iv topoisomerase mutations in clinical isolates of morganella morganii harbouring the qnrd gene. | the aim of this study was to show the emergence of the qnrd gene among fluoroquinolone-resistant morganella morganii isolate. the occurrence of mutations in dna gyrase (gyra and gyrb) and topoisomerase iv (parc,pare) genes was also investigated in this strain. | 2014 | 25106550 |
| antibiotic management of complicated intra-abdominal infections in adults: the asian perspective. | regional epidemiological data and resistance profiles are essential for selecting appropriate antibiotic therapy for intra-abdominal infections (iais). however, such information may not be readily available in many areas of asia and current international guidelines on antibiotic therapy for iais are for western countries, with the most recent guidance for the asian region dating from 2007. therefore, the asian consensus taskforce on complicated intra-abdominal infections (act-ciai) was convened ... | 2014 | 25568794 |
| emerging trends in invasive and noninvasive isolates of streptococcus agalactiae in a latin american hospital: a 17-year study. | streptococcus agalactiae or group b streptococcus (gbs) has been recognized as a lethal pathogen in neonates worldwide. s. agalactiae infections also severely affect pregnant women and immunosuppressed adults with substantial attributable morbidity and mortality. however, in latin america, studies on the epidemiology and behaviour of s. agalactiae infections remain limited. | 2014 | 25086463 |
| development of a rapid microarray-based dna subtyping assay for the alleles of shiga toxins 1 and 2 of escherichia coli. | in this study, we developed a new rapid, economic, and automated microarray-based genotyping test for the standardized subtyping of shiga toxins 1 and 2 of escherichia coli. the microarrays from alere technologies can be used in two different formats, the arraytube and the arraystrip (which enables high-throughput testing in a 96-well format). one microarray chip harbors all the gene sequences necessary to distinguish between all stx subtypes, facilitating the identification of single and multip ... | 2014 | 24899022 |
| comparative evaluation of two chromogenic tests for rapid detection of carbapenemase in enterobacteriaceae and in pseudomonas aeruginosa isolates. | we compared the performance of the carba np test and the rosco rapid carb screen kit for detecting carbapenemase-producing enterobacteriaceae and pseudomonas aeruginosa. both tests are rapid and highly sensitive; however, the carba np test showed superior specificity, and several uninterpretable results were observed with the rapid carb screen. | 2014 | 24850357 |
| a rapid matrix-assisted laser desorption ionization-time of flight mass spectrometry-based method for single-plasmid tracking in an outbreak of carbapenem-resistant enterobacteriaceae. | carbapenem-resistant enterobacteriaceae (cre) have spread globally and represent a serious and growing threat to public health. rapid methods for tracking plasmids carrying carbapenemase genes could greatly benefit infection control efforts. here, we demonstrate that real-time, direct tracking of a single plasmid in a bacterial strain responsible for an outbreak is possible using a commercial matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) system. in t ... | 2014 | 24850353 |
| high incidence and endemic spread of ndm-1-positive enterobacteriaceae in henan province, china. | the emergence and spread of new delhi metallo-β-lactamase 1 (ndm-1)-producing carbapenem-resistant enterobacteriaceae (cre) present an urgent threat to human health. in china, the bla(ndm-1 gene has been reported mostly in acinetobacter spp. but is rarely found in enterobacteriaceae. here, we report a high incidence and endemic spread of ndm-1-producing cre in henan province in china. sixteen (33.3%) of the 48 cre isolates obtained from patients during june 2011 to july 2012 were positive for bl ... | 2014 | 24777095 |
| complete sequencing of inci1 sequence type 2 plasmid pjie512b indicates mobilization of blacmy-2 from an inca/c plasmid. | sequencing of pjie512b, a 92.3-kb inci1 sequence type 2 (st2) plasmid carrying bla(cmy-2), revealed a bla(cmy-2) context that appeared to have been mobilized from an inca/c plasmid by the insertion sequence is1294. a comparison with published plasmids suggests that bla(cmy-2) has been mobilized from inca/c to inci1 plasmids more than once by is1294-like elements. alignment of pjie512b with the only other available inci1 st2 plasmid revealed differences across the backbones, indicating variabilit ... | 2014 | 24890591 |
| the occurrence of enterobacteriaceae producing kpc carbapenemases in a general hospital in curacao. | although the presence of carbapenemase-producing enterobacteriaceae (cpe) are extensively documented in north and south america. cpe have not been reported from curacao. however, recently intercontinental spread was suggested of a kpc carbapenemase producing klebsiella pneumoniae in a patient in the united kingdom with previous admission to a hospital in curacao in 2009. | 2014 | 25132965 |
| klebsiella: a long way to go towards understanding this enigmatic jet-setter. | klebsiella pneumoniae is the causative agent of a variety of diseases, including pneumonia, urinary tract infections, septicemia, and the recently recognized pyogenic liver abscesses (pla). renewed efforts to identify and understand the bacterial determinants required to cause disease have come about because of the worldwide increase in the isolation of strains resistant to a broad spectrum of antibiotics. the recent increased isolation of carbapenem-resistant strains further reduces the availab ... | 2014 | 25165563 |
| rapid identification of carbapenemase genes in gram-negative bacteria with an oligonucleotide microarray-based assay. | rapid molecular identification of carbapenemase genes in gram-negative bacteria is crucial for infection control and prevention, surveillance and for epidemiological purposes. furthermore, it may have a significant impact upon determining the appropriate initial treatment and greatly benefit for critically ill patients. a novel oligonucleotide microarray-based assay was developed to simultaneously detect genes encoding clinically important carbapenemases as well as selected extended (esbl) and n ... | 2014 | 25068267 |
| aerobic bacterial community of american cockroach periplaneta americana,a step toward finding suitable paratransgenesis candidates. | cockroaches mechanically spread pathogenic agents, however, little is known about their gut microbiota. identification of midgut microbial community helps targeting novel biological control strategies such as paratransgenesis. here the bacterial microbiota of periplaneta americana midgut, were identified and evaluated for finding proper paratransgenesis candidate. | 2014 | 26114142 |
| aerobic bacterial community of american cockroach periplaneta americana,a step toward finding suitable paratransgenesis candidates. | cockroaches mechanically spread pathogenic agents, however, little is known about their gut microbiota. identification of midgut microbial community helps targeting novel biological control strategies such as paratransgenesis. here the bacterial microbiota of periplaneta americana midgut, were identified and evaluated for finding proper paratransgenesis candidate. | 2014 | 26114142 |
| polymicrobial bloodstream infections in the neonatal intensive care unit are associated with increased mortality: a case-control study. | polymicrobial infections in adults and children are associated with increase in mortality, duration of intensive care and healthcare costs. very few studies have characterized polymicrobial bloodstream infections in the neonatal unit. considerable variation has been reported in incidence of polymicrobial infections and associated clinical outcomes. we characterized the risk factors and outcomes of polymicrobial bloodstream infections in our neonatal units in a tertiary hospital in north america. | 2014 | 25022748 |
| multiresistant extended-spectrum β-lactamase-producing enterobacteriaceae from humans, companion animals and horses in central hesse, germany. | multiresistant gram-negative bacteria producing extended-spectrum β-lactamases (esbls) are an emerging problem in human and veterinary medicine. this study focused on comparative molecular characterization of β-lactamase and esbl-producing enterobacteriaceae isolates from central hesse in germany. isolates originated from humans, companion animals (dogs and cats) and horses. | 2014 | 25014994 |
| anti-fungal activities of medicinal plants extracts of ivorian pharmacopoeia. | this study was to evaluate in vitro anti-fungal activity of aqueous and hydroethanolic from medicinal plants extracts collected in côte d'ivoire. | 2014 | 26401367 |
| simulate_pcr for amplicon prediction and annotation from multiplex, degenerate primers and probes. | pairing up primers to amplify desired targets and avoid undesired cross reactions can be a combinatorial challenge. effective prediction of specificity and inclusivity from multiplexed primers and taqman®/luminex® probes is a critical step in pcr design. | 2014 | 25005023 |
| identification of a two-component fatty acid kinase responsible for host fatty acid incorporation by staphylococcus aureus. | extracellular fatty acid incorporation into the phospholipids of staphylococcus aureus occurs via fatty acid phosphorylation. we show that fatty acid kinase (fak) is composed of two dissociable protein subunits encoded by separate genes. faka provides the atp binding domain and interacts with two distinct fakb proteins to produce acyl-phosphate. the fakbs are fatty acid binding proteins that exchange bound fatty acid/acyl-phosphate with fatty acid/acyl-phosphate presented in detergent micelles o ... | 2014 | 25002480 |
| antibiotic susceptibility profile of bacteria isolated from natural sources of water from rural areas of east sikkim. | contamination of water, food, and environment with antibiotic-resistant bacteria poses a serious public health issue. | 2014 | 25136156 |
| recurrent cholangitis by biliary stasis due to non-obstructive afferent loop syndrome after pylorus-preserving pancreatoduodenectomy: report of a case. | we report a 71-year-old man who had undergone pylorus-preserving pancreatoduodenectomy (pppd) using pppd-iv reconstruction for cholangiocarcinoma. for 6 years thereafter, he had suffered recurrent cholangitis, and also a right liver abscess (s5/8), which required percutaneous drainage at 9 years after pppd. at 16 years after pppd, he had been admitted to the other hospital because of acute purulent cholangitis. although medical treatment resolved the cholangitis, the patient was referred to our ... | 2014 | 25058778 |
| rapid diagnosis of streptococcus pneumoniae-induced haemolytic-uraemic syndrome. | 2014 | 24887232 | |
| comparison of antimicrobial sensitivity to older and newer quinolones versus piperacillin-tazobactam, cefepime and meropenem in febrile patients with cancer in two referral pediatric centers in tehran, iran. | infection in pediatric cancer patients has become a concerning problem due to increasing antimicrobial resistance. the goal of this study was to determine the antimicrobial resistance patterns of blood isolates from pediatric oncology patients in iran to determine if there was significant resistance to quinolones. | 2014 | 25045453 |
| isolation and identification of an enterobacter cloacae strain producing a novel subtype of shiga toxin type 1. | we describe here the isolation and identification of a shiga toxin 1 (stx1)-producing enterobacter cloacae strain, m12x01451, from a human clinical specimen. the bacterial isolate was identified as e. cloacae using a polyphasic approach that included phenotypic, genetic, and proteomic analyses. the m12x01451 stx1 was sequenced, and the holotoxin was found to share only 87% amino acid sequence identity with the nearest stx1 subtype reference sequence. sequence analysis of the regions immediately ... | 2014 | 24759708 |
| multiplex pcr for identification of two capsular types in epidemic kpc-producing klebsiella pneumoniae sequence type 258 strains. | we developed a multiplex pcr assay capable of identifying two capsular polysaccharide synthesis sequence types (sequence type 258 [st258] cps-1 and cps-2) in epidemic klebsiella pneumoniae st258 strains. the assay performed with excellent sensitivity (100%) and specificity (100%) for identifying cps types in 60 st258 k. pneumoniae sequenced isolates. the screening of 419 st258 clonal isolates revealed a significant association between cps type and k. pneumoniae carbapenemase (kpc) variant: cps-1 ... | 2014 | 24733470 |
| fructose-asparagine is a primary nutrient during growth of salmonella in the inflamed intestine. | salmonella enterica serovar typhimurium (salmonella) is one of the most significant food-borne pathogens affecting both humans and agriculture. we have determined that salmonella encodes an uptake and utilization pathway specific for a novel nutrient, fructose-asparagine (f-asn), which is essential for salmonella fitness in the inflamed intestine (modeled using germ-free, streptomycin-treated, ex-germ-free with human microbiota, and il10-/- mice). the locus encoding f-asn utilization, fra, provi ... | 2014 | 24967579 |
| impact of appropriate antimicrobial treatment on transition from ventilator-associated tracheobronchitis to ventilator-associated pneumonia. | two small randomized controlled trials have suggested beneficial effects of antibiotic treatment in patients with ventilator-associated tracheobronchitis (vat). the primary aim of this study is to determine the impact of appropriate antibiotic treatment on transition from vat to ventilator-associated pneumonia (vap) in critically ill patients. the secondary objective was to determine the incidence of vap in patients with vat. | 2014 | 24958136 |
| conservation of the low-shear modeled microgravity response in enterobacteriaceae and analysis of the trp genes in this response. | low fluid shear force, including that encountered in microgravity models, induces bacterial responses, but the range of bacteria capable of responding to this signal remains poorly characterized. we systematically analyzed a range of gram negative enterobacteriaceae for conservation of the low-shear modeled microgravity (lsmmg) response using phenotypic assays, qpcr, and targeted mutations. our results indicate lsmmg response conservation across enterobacteriacae with potential variance in up- o ... | 2014 | 25006354 |
| hygienic-sanitary evaluation of sushi and sashimi sold in messina and catania, italy. | sushi and sashimi are traditional japanese food, mostly consisting of raw seafood alone or in combination with rice. eating sushi and sashimi has become popular in many countries even outside japan. this food is not free from health risks such as ingestion of pathogenic bacteria or parasite. the aim of this study was to investigate on hygienic-sanitary quality of sushi and sashimi sold in the cities of messina and catania, southern italy. fifty samples (38 sushi and 12 sashimi) were analysed to ... | 2014 | 27800343 |
| β-carboxysomal proteins assemble into highly organized structures in nicotiana chloroplasts. | the photosynthetic efficiency of c3 plants suffers from the reaction of ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) with o2 instead of co2 , leading to the costly process of photorespiration. increasing the concentration of co2 around rubisco is a strategy used by photosynthetic prokaryotes such as cyanobacteria for more efficient incorporation of inorganic carbon. engineering the cyanobacterial co2 -concentrating mechanism, the carboxysome, into chloroplasts is an approach to enha ... | 2014 | 24810513 |
| phenotypic and molecular characterization of plasmid mediated ampc β-lactamases among escherichia coli, klebsiella spp., and proteus mirabilis isolated from urinary tract infections in egyptian hospitals. | the incidence of resistance by enterobacteriaceae to β-lactam/β-lactamase inhibitors combination is increasing in egypt. three phenotypic techniques, comprising ampc disk diffusion and inhibition dependent methods using phenylboronic acid (pba) and cloxacillin, were compared to pcr based method for detection of plasmid mediated ampc β-lactamase in common urinary tract isolates. a total of 143 isolates, including e. coli, klebsiella pneumonia, and proteus mirabilis, were collected from urinary tr ... | 2014 | 25003107 |
| characterization of shiga toxin-producing escherichia coli isolated from ground beef collected in different socioeconomic strata markets in buenos aires, argentina. | consumption of raw/undercooked ground beef is the most common route of transmission of shiga toxin-producing e. coli (stec). the aim of the study was to determine the stec contamination level of the ground beef samples collected in 36 markets of different socioeconomic strata in buenos aires, argentina, and the characterization of the isolated strains. ninety-one out of 252 (36.1%) samples were stx+. fifty-seven stec strains were recovered. eleven stec strains belonged to o157 serogroup, and 46 ... | 2014 | 25006586 |
| a case of extravascular hemolysis with tk-activation. | a 50-year-old female with ovarian cancer for 4 years presented with abdominal pain. she started antibiotics for possible infection, and developed extravascular hemolysis. all antigen detection tests were negative, but lectin panel suggested tk-activation. additional laboratory testing in conjunction with blood bank is essential to investigate rare cause of hemolysis. | 2014 | 25356271 |
| activities of ceftazidime and avibactam against β-lactamase-producing enterobacteriaceae in a hollow-fiber pharmacodynamic model. | avibactam is a novel non-β-lactam β-lactamase inhibitor that is currently undergoing phase 3 clinical trials in combination with ceftazidime. ceftazidime is hydrolyzed by a broad range of β-lactamases, but avibactam is able to inhibit the majority of these enzymes. the studies described here attempt to provide insight into the amount of avibactam required to suppress bacterial growth in an environment where the concentrations of both agents are varying as they would when administered to humans. ... | 2014 | 24687507 |
| safety and efficacy of long-term outpatient ertapenem therapy. | ertapenem is increasingly utilized in outpatient parenteral antimicrobial therapy (opat), but data regarding the efficacy and safety of long-term ertapenem therapy have been limited. we conducted a retrospective cohort study of adult patients who received outpatient ertapenem therapy at our center between 2010 and 2013. among 306 unique patients who were discharged on ertapenem therapy, the most common indications were intra-abdominal infections (38%), followed by pneumonia (12%), bone and joint ... | 2014 | 24709258 |
| fosfomycin susceptibility in carbapenem-resistant enterobacteriaceae from germany. | due to the increase in multidrug-resistant enterobacteriaceae, the interest in older antimicrobial agents, like fosfomycin, has increased. in this study, we used agar dilution for testing susceptibilities to fosfomycin in a collection of 107 carbapenem-nonsusceptible enterobacteriaceae isolates, of which 80 produced various types of carbapenemases, including kpc, vim, ndm, and oxa-48. overall, 78% of the strains had fosfomycin mics of ≤ 32 mg/liter and were thus considered to be susceptible acco ... | 2014 | 24648559 |
| a recent review on phytochemical constituents and medicinal properties of kesum (polygonum minus huds.). | medicinal plants and herbal preparations are gaining renowned interest in scientific communities nowadays due to their reliable pharmacological actions and affordability to common people which makes them effective in control of various diseases. polygonum minus (polygonaceae) locally known as kesum is an aromatic plant commonly used in malay delicacies. the plant is having potential applications due to its high volatile oil constituents in perfumes and powerful antioxidant activity. it has been ... | 2014 | 25182942 |
| description of a systematic pharmaceutical care approach intended to increase the appropriateness of medication use by elderly patients. | the pharmaceutical care practice represents a model of responsible pharmacist involvement in the pharmacotherapy optimization of various population groups, including the elderly, known to be at risk for drug-related problems. romanian pharmacists could use validated pharmaceutical care experiences to confirm their role as health-care professionals. this descriptive research presents the application in two real and different environments of practice of a structured pharmaceutical care approach co ... | 2014 | 26528011 |
| structural insights into higher order assembly and function of the bacterial microcompartment protein pdua. | bacterial microcompartments are large proteinaceous assemblies that are found in the cytoplasm of some bacteria. these structures consist of proteins constituting a shell that houses a number of enzymes involved in specific metabolic processes. the 1,2-propanediol-utilizing microcompartment is assembled from seven different types of shell proteins, one of which is pdua. it is one of the more abundant components of the shell and intriguingly can form nanotubule-like structures when expressed on i ... | 2014 | 24873823 |
| autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of bifidobacterium breve ucc2003. | in the present study we show that luxs of bifidobacterium breve ucc2003 is involved in the production of the interspecies signaling molecule autoinducer-2 (ai-2), and that this gene is essential for gastrointestinal colonization of a murine host, while it is also involved in providing protection against salmonella infection in caenorhabditis elegans. we demonstrate that a b. breve luxs-insertion mutant is significantly more susceptible to iron chelators than the wt strain and that this sensitivi ... | 2014 | 24871429 |
| holoptelea integrifolia (roxb.) planch: a review of its ethnobotany, pharmacology, and phytochemistry. | holoptelea integrifolia (ulmaceae) is a versatile medicinal plant used in various indigenous systems of medicine for curing routine healthcare maladies. it is traditionally used in the treatment and prevention of several ailments like leprosy, inflammation, rickets, leucoderma, scabies, rheumatism, ringworm, eczema, malaria, intestinal cancer, and chronic wounds. in vitro and in vivo pharmacological investigations on crude extracts and isolated compounds showed antibacterial, antifungal, analges ... | 2014 | 24949441 |
| structural and biochemical characterization reveals lysgh15 as an unprecedented "ef-hand-like" calcium-binding phage lysin. | the lysin lysgh15, which is derived from the staphylococcal phage gh15, demonstrates a wide lytic spectrum and strong lytic activity against methicillin-resistant staphylococcus aureus (mrsa). here, we find that the lytic activity of the full-length lysgh15 and its chap domain is dependent on calcium ions. to elucidate the molecular mechanism, the structures of three individual domains of lysgh15 were determined. unexpectedly, the crystal structure of the lysgh15 chap domain reveals an "ef-hand- ... | 2014 | 24831957 |
| chemical composition and antibacterial activity of essential oils from the tunisian allium nigrum l. | the chemical composition of the essential oils of different allium nigrum l. organs and the antibacterial activity were evaluated. the study is particularly interesting because hitherto there are no reports on the antibacterial screening of this species with specific chemical composition. therefore, essential oils from different organs (flowers, stems, leaves and bulbs) obtained separately by hydrodistillation were analyzed using gas chromatography-mass spectrometry (gc-ms). the antibacterial ac ... | 2014 | 26417280 |
| role of novel multidrug efflux pump involved in drug resistance in klebsiella pneumoniae. | multidrug resistant klebsiella pneumoniae have caused major therapeutic problems worldwide due to the emergence of the extended-spectrum β-lactamase producing strains. although there are >10 major facilitator super family (mfs) efflux pumps annotated in the genome sequence of the k. pneumoniae bacillus, apparently less is known about their physiological relevance. | 2014 | 24823362 |
| duct ectasia and periductal mastitis in indian women. | there is very little awareness of the general physicians and surgeons about the benign breast conditions such as duct ectasia (de) and periductal mastitis (pdm) causing nipple discharge. not only that these benign breast diseases ring a false alarm of cancer, they are also the second most common cause of benign breast diseases. the objective was to study the clinical and microbiological profiles of duct ectasia and periductal mastitis in indian women for better understanding of the disease proce ... | 2014 | 27011490 |
| duct ectasia and periductal mastitis in indian women. | there is very little awareness of the general physicians and surgeons about the benign breast conditions such as duct ectasia (de) and periductal mastitis (pdm) causing nipple discharge. not only that these benign breast diseases ring a false alarm of cancer, they are also the second most common cause of benign breast diseases. the objective was to study the clinical and microbiological profiles of duct ectasia and periductal mastitis in indian women for better understanding of the disease proce ... | 2014 | 27011490 |
| high dose tigecycline in critically ill patients with severe infections due to multidrug-resistant bacteria. | the high incidence of multidrug-resistant (mdr) bacteria among patients admitted to icus has determined an increase of tigecycline (tgc) use for the treatment of severe infections. many concerns have been raised about the efficacy of this molecule and increased dosages have been proposed. our purpose is to investigate tgc safety and efficacy at higher than standard doses. | 2014 | 24887101 |
| phenotypic, molecular and antimicrobial susceptibility assessment in isolates from chronic ulcers of cured leprosy patients: a case study in southern brazil. | one of the most stigmatizing physical sequelae of leprosy in cured patients is the development of chronic lower extremity ulcers. the bacterial diversity present in ulcers is considered one of the factors that can delay the healing process, as well as serve as a focus for severe secondary infections. | 2014 | 24937812 |
| rapid detection of β-lactamase-hydrolyzing extended-spectrum cephalosporins in enterobacteriaceae by use of the new chromogenic βlacta test. | the chromogenic βlacta test developed for the rapid detection of β-lactamase-hydrolyzing extended-spectrum cephalosporins in enterobacteriaceae revealed good performance with extended-spectrum β-lactamase (esbl) producers (97.5% true-positive results). however, false-negative results occurred with chromosomal ampc hyperproducers and plasmid ampc producers, whereas uninterpretable results were mostly due to vim-1 carbapenemase producers and possibly low levels of expressed esbls. | 2014 | 24574293 |
| travel-related carbapenemase-producing gram-negative bacteria in alberta, canada: the first 3 years. | we describe here the characteristics of alberta, canada, patients with infections or colonizations with carbapenemase-producing gram-negative bacteria during 2010 to 2013 that were linked to recent travel outside canada. antimicrobial susceptibility was determined by broth microdilution, and isolates were characterized using pcr, sequencing, and multilocus sequencing typing. a broth mating study was used to assess the transferability of resistance plasmids, which were subsequently characterized. ... | 2014 | 24599977 |
| dissemination of 16s rrna methylase arma-producing acinetobacter baumannii and emergence of oxa-72 carbapenemase coproducers in japan. | forty-nine clinical isolates of multidrug-resistant acinetobacter baumannii were obtained from 12 hospitals in 7 prefectures throughout japan. molecular phylogenetic analysis revealed the clonal spread of a. baumannii sequence type 208 (st208) and st455 isolates harboring the arma gene and st512 harboring the arma and blaoxa-72 genes. these findings show that a. baumannii isolates harboring arma are disseminated throughout japan, and this is the first report to show that a. baumannii strains har ... | 2014 | 24550340 |
| spread of ndm-1-producing enterobacteriaceae in a neonatal intensive care unit in istanbul, turkey. | twenty-two consecutive carbapenem-resistant enterobacterial isolates were recovered from patients hospitalized between january and april 2013 in different units at a university hospital in istanbul, turkey. these were klebsiella pneumoniae isolates producing the carbapenemases oxa-48, ndm-1, and kpc-2, enterobacter cloacae isolates producing ndm-1, and escherichia coli isolates producing oxa-48. most of the oxa-48-producing k. pneumoniae and all the ndm-1-producing e. cloacae were clonally relat ... | 2014 | 24550328 |
| identification of cmy-2-type cephalosporinases in clinical isolates of enterobacteriaceae by maldi-tof ms. | this study exploited the possibility to detect citrobacter freundii-derived cmy-2-like cephalosporinases in enterobacteriaceae clinical isolates using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms). periplasmic proteins were prepared using a modified sucrose method and analyzed by maldi-tof ms. a ca. 39,850-m/z peak, confirmed to represent a c. freundii-like β-lactamase by in-gel tryptic digestion followed by maldi-tof/tof ms, was observed only in cmy ... | 2014 | 24566177 |
| comparative analysis of virulence and resistance profiles of salmonella enteritidis isolates from poultry meat and foodborne outbreaks in northern jordan. | this study was conducted to isolate salmonella enteritidis from poultry samples and compare their virulence and antibiotic resistance profiles to s. enteritidis isolated from outbreaks in northern jordan. two hundred presumptive isolates were obtained from 302 raw poultry samples and were subjected to further analysis and confirmation. a phylogenic tree based on 16s rrna sequencing was constructed and selected isolates representing each cluster were further studied for their virulence in normal ... | 2014 | 24780883 |
| quinolone resistance mechanisms among extended-spectrum beta-lactamase (esbl) producing escherichia coli isolated from rivers and lakes in switzerland. | sixty extended-spectrum β-lactamase (esbl)-producing escherichia coli isolated from rivers and lakes in switzerland were screened for individual strains additionally exhibiting a reduced quinolone susceptibility phenotype. totally, 42 such isolates were found and further characterized for their molecular (fluoro)quinolone resistance mechanisms. pcr and sequence analysis were performed to identify chromosomal mutations in the quinolone resistance-determining regions (qrdr) of gyra, gyrb, parc and ... | 2014 | 24755830 |
| class 1 integrons and antibiotic resistance of clinical acinetobacter calcoaceticus-baumannii complex in poznań, poland. | sixty-three clinical isolates of acinetobacter calcoaceticus-baumannii complex were analyzed for the presence of integrons and antimicrobial resistance. class 1 integrons were detected in 40 (63.5 %) isolates. none of them had class 2 or class 3 integrons. the majority of the integrons contained aacc1-orfa-orfb-aada1 gene cassette array. the presence of integrons was associated with the increased frequency of resistance to 12 of 15 antimicrobials tested, multi-drug resistance phenotype, and the ... | 2014 | 24740302 |
| study of bacterial meningitis in children below 5 years with comparative evaluation of gram staining, culture and bacterial antigen detection. | bacterial meningitis is one of the most serious infections seen in infants and children, which is associated with acute complications and chronic morbidity. infections of central nervous system (cns) still dominate the scene of childhood neurological disorders in most of the developing tropical countries. | 2014 | 24959439 |
| the gut microbial community of midas cichlid fish in repeatedly evolved limnetic-benthic species pairs. | gut bacterial communities are now known to influence a range of fitness related aspects of organisms. but how different the microbial community is in closely related species, and if these differences can be interpreted as adaptive is still unclear. in this study we compared microbial communities in two sets of closely related sympatric crater lake cichlid fish species pairs that show similar adaptations along the limnetic-benthic axis. the gut microbial community composition differs in the speci ... | 2014 | 24733403 |
| evaluation of an automated rapid diagnostic assay for detection of gram-negative bacteria and their drug-resistance genes in positive blood cultures. | we evaluated the performance of the verigene gram-negative blood culture nucleic acid test (bc-gn; nanosphere, northbrook, il, usa), an automated multiplex assay for rapid identification of positive blood cultures caused by 9 gram-negative bacteria (gnb) and for detection of 9 genes associated with β-lactam resistance. the bc-gn assay can be performed directly from positive blood cultures with 5 minutes of hands-on and 2 hours of run time per sample. a total of 397 gnb positive blood cultures we ... | 2014 | 24705449 |
| bacterial load of german cockroach (blattella germanica) found in hospital environment. | cockroaches are among the most common pests in public dwellings and health facilities. their presence can raise safety concerns, especially as they maybe carriers of pathogenic organisms. | 2014 | 24766338 |
| surgical site infections in genital reconstruction surgery for gender reassignment, detroit: 1984-2008. | gender reassignment surgery (i.e., male-to-female or female-to-male) entails a series of complex surgical procedures. we conducted a study to explore epidemiologic characteristics of patients who underwent genital reconstruction operations as components of gender reassignment and to analyze risk factors for surgical-site infections (ssis) following these operations. | 2014 | 24180342 |
| ventilator-associated pneumonia in a tertiary care intensive care unit: analysis of incidence, risk factors and mortality. | ventilator-associated pneumonia (vap) is the most common nosocomial infection diagnosed in the intensive care unit (icu) and in spite of advances in diagnostic techniques and management it remains a common cause of hospital morbidity and mortality. | 2014 | 24872648 |
| variation in potency and spectrum of tigecycline activity against bacterial strains from u.s. medical centers since its approval for clinical use (2006 to 2012). | tigecycline was initially approved by the u.s. food and drug administration (fda) in june 2005. we assessed the evolution of tigecycline in vitro activities since the initial approval of tigecycline for clinical use by analyzing the results of 7 years (2006 to 2012) of data from the sentry antimicrobial surveillance program in the united states. we also analyzed trends over time for key resistance phenotypes. the analyses included 68,608 unique clinical isolates collected from 29 medical centers ... | 2014 | 24492361 |
| strategy for rapid detection of carbapenemase-producing enterobacteriaceae. | a prospective survey was conducted on 862 enterobacteriaceae isolates with reduced susceptibility to carbapenems. the carba np test, uv spectrophotometry, and a dna microarray were used to detect carbapenemase producers, and the results were compared to those from pcr and sequencing. the 172 carbapenemase producers were detected using the carba np test and uv spectrophotometry, whereas the dna microarray failed to detect imi producers. the use of the carba np test as a first screening, followed ... | 2014 | 24468779 |
| enterobacteriaceae with extended-spectrum- and pampc-type β-lactamase-encoding genes isolated from freshwater fish from two lakes in switzerland. | 2014 | 24449774 | |
| oxa β-lactamases. | the oxa β-lactamases were among the earliest β-lactamases detected; however, these molecular class d β-lactamases were originally relatively rare and always plasmid mediated. they had a substrate profile limited to the penicillins, but some became able to confer resistance to cephalosporins. from the 1980s onwards, isolates of acinetobacter baumannii that were resistant to the carbapenems emerged, manifested by plasmid-encoded β-lactamases (oxa-23, oxa-40, and oxa-58) categorized as oxa enzymes ... | 2014 | 24696435 |
| role of pseudomonas aeruginosa ampr on β-lactam and non-β-lactam transient cross-resistance upon pre-exposure to subinhibitory concentrations of antibiotics. | pseudomonas aeruginosa is one of the most dreaded opportunistic pathogens accounting for 10 % of hospital-acquired infections, with a 50 % mortality rate in chronically ill patients. the increased prevalence of drug-resistant isolates is a major cause of concern. resistance in p. aeruginosa is mediated by various mechanisms, some of which are shared among different classes of antibiotics and which raise the possibility of cross-resistance. the goal of this study was to explore the effect of subi ... | 2014 | 24464693 |
| integrons in the intestinal microbiota as reservoirs for transmission of antibiotic resistance genes. | the human intestinal microbiota plays a major beneficial role in immune development and resistance to pathogens. the use of antibiotics, however, can cause the spread of antibiotic resistance genes within the resident intestinal microbiota. important vectors for this are integrons. this review therefore focuses on the integrons in non-pathogenic bacteria as a potential source for the development and persistence of multidrug resistance. integrons are a group of genetic elements which are assembly ... | 2014 | 25437798 |
| clinical analysis of pulmonary infection in hemodialysis patients. | the present study aimed to investigate the pathogen distribution and drug resistance of lung infections in hemodialysis to guide clinical empirical pharmacy. the clinical data of 116 hemodialysis patients with pulmonary infection were analyzed. the majority of the 82 pathogens isolated from the sputa of patients were gram-negative bacteria (accounting for 71.95%). the results of the drug sensitivity test suggested that gram-negative bacilli had low resistance rates to piperacillin and tazobactam ... | 2014 | 24926372 |
| worldwide dissemination of the ndm-type carbapenemases in gram-negative bacteria. | the emergence of one of the most recently described carbapenemases, namely, the new delhi metallo-lactamase (ndm-1), constitutes a critical and growingly important medical issue. this resistance trait compromises the efficacy of almost all lactams (except aztreonam), including the last resort carbapenems. therapeutical options may remain limited mostly to colistin, tigecycline, and fosfomycin. the main known reservoir of ndm producers is the indian subcontinent whereas a secondary reservoir seem ... | 2014 | 24790993 |
| cerium oxide and iron oxide nanoparticles abolish the antibacterial activity of ciprofloxacin against gram positive and gram negative biofilm bacteria. | metal oxide nanoparticles have been suggested as good candidates for the development of antibacterial agents. cerium oxide (ceo2) and iron oxide (fe2o3) nanoparticles have been utilized in a number of biomedical applications. here, the antibacterial activity of ceo2 and fe2o3 nanoparticles were evaluated on a panel of gram positive and gram negative bacteria in both the planktonic and biofilm cultures. additionally, the effect of combining ceo2 and fe2o3 nanoparticles with the broad spectrum ant ... | 2014 | 24643389 |
| cerium oxide and iron oxide nanoparticles abolish the antibacterial activity of ciprofloxacin against gram positive and gram negative biofilm bacteria. | metal oxide nanoparticles have been suggested as good candidates for the development of antibacterial agents. cerium oxide (ceo2) and iron oxide (fe2o3) nanoparticles have been utilized in a number of biomedical applications. here, the antibacterial activity of ceo2 and fe2o3 nanoparticles were evaluated on a panel of gram positive and gram negative bacteria in both the planktonic and biofilm cultures. additionally, the effect of combining ceo2 and fe2o3 nanoparticles with the broad spectrum ant ... | 2014 | 24643389 |
| detection and occurrence of plasmid-mediated ampc in highly resistant gram-negative rods. | the aim of this study was to compare the current screening methods and to evaluate confirmation tests for phenotypic plasmidal ampc (pampc) detection. | 2014 | 24642853 |
| insusceptibility to disinfectants in bacteria from animals, food and humans-is there a link to antimicrobial resistance? | enterococcus faecalis (n = 834) and enterococcus faecium (n = 135) from blood and feces of hospitalized humans, from feces of outpatients and livestock and from food were screened for their susceptibility to a quaternary ammonium compound (didecyldimethylammoniumchloride, ddac) and to 28 antibiotics by micro-/macrodilution. the maximum ddac-mic in our field study was 3.5 mg/l, but after adaptation in the laboratory, mic values of 21.9 mg/l were observed. strains for which ddac had mics > 1.4 mg/ ... | 2014 | 24672513 |
| identification of phenolic compounds and evaluation of antioxidant and antimicrobial properties of euphorbia tirucalli l. | bioactive compounds extracted from natural sources can benefit human health. the aim of this work was to determine total phenolic content and antioxidant activity in extracts of euphorbia tirucalli l. followed by identification and quantification of the phenolic compounds, as well as their antibacterial activities. antioxidant activities were determined by dpph and abts(•+) assay. identification of phenolic compounds was performed using high-performance liquid chromatography (hplc), and antimicr ... | 2014 | 26784670 |
| genome sequencing, annotation of citrobacter freundii strain gtc 09479. | we report the 4.9-mb genome sequence of citrobacter freundii strain gtc 09479, isolated from urine sample collected during the year 1983 at gifu university graduate school of medicine, japan. this draft genome consist of 4,899,578 bp with 51.62% g + c, 4,574 predicted cdss, 72 trnas and 10 rrnas. | 2014 | 26484065 |
| the transcription factor gata3 is critical for the development of all il-7rα-expressing innate lymphoid cells. | innate lymphoid cells (ilcs) are critical in innate immune responses to pathogens and lymphoid organ development. similar to cd4(+) t helper (th) cell subsets, ilc subsets positive for interleukin-7 receptor α (il-7rα) produce distinct sets of effector cytokines. however, the molecular control of il-7rα(+) ilc development and maintenance is unclear. here, we report that gata3 was indispensable for the development of all il-7rα(+) ilc subsets and t cells but was not required for the development o ... | 2014 | 24631153 |
| a culture-based method for determining the production of secreted protease inhibitors. | we have developed a culture-based method for determining the production of secreted protease inhibitors. the assay utilizes standard proteolysis detection plates to support microbial growth followed by infiltrating the plate with a protease and subsequently detecting the remaining protein by trichloroacetic acid (tca) precipitation, or by bromocreosol green (bcg) or ponseau s (ps) staining. the presence of a protease inhibitor can be observed in the form of a protected zone of protein around the ... | 2014 | 24632514 |
| clinical manifestations of bacteremia caused by aeromonas species in southern taiwan. | this study is conducted to investigate the clinical characteristics of patients with bacteremia caused by aeromonas species. | 2014 | 24614100 |
| sequential isolation in a patient of raoultella planticola and escherichia coli bearing a novel iscr1 element carrying blandm-1. | the gene for new delhi metallo-β-lactamase 1 (ndm-1) has been reported to be transmitted via plasmids which are easily transferable and capable of wide distribution. we report the isolation of two ndm-1 producing strains and possible in vivo transfer of blandm-1 in a patient. | 2014 | 24594606 |
| activities of selected medicinal plants against multi-drug resistant gram-negative bacteria in cameroon. | medicinal plants are used worldwide for several human ailments including bacterial infections. the present work was designed to assess the in vitro antibacterial activities of some cameroonian medicinal plants including entada abyssinica, entada africana, pentaclethra macrophylla, allexis cauliflora, anthocleista leibrechtsiana, carapa procera, carica papaya and persea americana against gram-negative bacteria expressing multidrug resistant (mdr) phenotypes. | 2014 | 26060474 |
| widespread antibiotic resistance of diarrheagenic escherichia coli and shigella species. | antibiotic resistance of enteric pathogens particularly shigella species, is a critical world-wide problem and monitoring their resistant pattern is essential, because the choice of antibiotics is absolutely dependent on regional antibiotic susceptibility patterns. during summer 2013, an unusual increase in number of diarrheal diseases was noticed in isfahan, a central province of iran. therefore, the antibiotic resistance of diarrheagenic escherichia coli and shigella species isolated were eval ... | 2014 | 25002896 |
| antibacterial activities of jatropha curcas (linn) on coliforms isolated from surface waters in akure, nigeria. | this study investigated the antibacterial activities of hot water, ethanol and acetone extracts of jatropha curcas (linn) leaves on coliforms isolated from surface waters using growth inhibition indices based on agar plate technique. the percentage recovery of the extracts was 19.17%, 18.10% and 18.80% for hot water, ethanol and acetone respectively. phytochemical screening of the extracts was also determined. qualitative phytochemical screening showed that the plant extracts contained steroids, ... | 2014 | 24711746 |
| role of ompa in the multidrug resistance phenotype of acinetobacter baumannii. | acinetobacter baumannii has emerged as a nosocomial pathogen with an increased prevalence of multidrug-resistant strains. the role of the outer membrane protein a (ompa) in antimicrobial resistance remains poorly understood. in this report, disruption of the ompa gene led to decreased mics of chloramphenicol, aztreonam, and nalidixic acid. we have characterized, for the first time, the contribution of ompa in the antimicrobial resistance phenotype of a. baumannii. | 2014 | 24379205 |
| efficacy of humanized carbapenem and ceftazidime regimens against enterobacteriaceae producing oxa-48 carbapenemase in a murine infection model. | enterobacteriaceae producing the oxa-48 carbapenemase are emerging worldwide, leaving few treatment options. efficacy has been demonstrated in vivo with ceftazidime against a ceftazidime-susceptible oxa-48 isolate but not with imipenem despite maintaining susceptibility. the relationship between phenotype and in vivo efficacy was assessed for oxa-48 producers using humanized regimens of 2 g doripenem every 8 h (q8h; 4 h infusion), 1 g ertapenem q24h, 2 g ceftazidime q8h (2 h inf), and 500 mg lev ... | 2014 | 24379200 |
| effects of egg shell membrane hydrolysates on anti-inflammatory, anti-wrinkle, anti-microbial activity and moisture-protection. | this study was conducted to examine the effects of eggshell membrane hydrolysates (esmh) on the anti-inflammatory, anti-wrinkle, anti-microbial activity, and moisture-protection for cosmetic use. whole esmh (before fractionation), and fraction i (>10 kda), fraction ii (3-10 kda), and fraction iii (<3 kda) of the hydrolysates were assessed in this experiment. as lipopolysaccharide (lps) and ifn-γ caused the inflammation on raw264.7 cell, whole esmh and fraction i showed to be effective in inhibit ... | 2014 | 26760742 |
| carbapenemase genes among multidrug resistant gram negative clinical isolates from a tertiary hospital in mwanza, tanzania. | the burden of antimicrobial resistance (amr) is rapidly growing across antibiotic classes, with increased detection of isolates resistant to carbapenems. data on the prevalence of carbapenem resistance in developing countries is limited; therefore, in this study, we determined the prevalence of carbapenemase genes among multidrug resistant gram negative bacteria (mdr-gnb) isolated from clinical specimens in a tertiary hospital in mwanza, tanzania. a total of 227 mdr-gnb isolates were analyzed fo ... | 2014 | 24707481 |
| emergence of escherichia coli producing extended-spectrum ampc β-lactamases (esac) in animals. | in both humans and animals, the spread of extended-spectrum β-lactamases (esbl)/ampc producers has become a major issue, particularly due to the plasmidic dissemination of most of these genes. besides, over-expression of the chromosomal ampc gene was largely reported in human and animal enterobacteriaceae and, more recently, modifications within the coding region of the ampc gene [encoding extended-spectrum ampc β-lactamases (esacs)] were shown to be responsible for an hydrolysis spectrum expand ... | 2014 | 24592257 |
| molecular characterization of esbl-producing enterobacteriaceae in northern portugal. | extended-spectrum β-lactamases (esbls) prevalence was studied in the north of portugal, among 193 clinical isolates belonging to citizens in a district in the boundaries between this country and spain from a total of 7529 clinical strains. in the present study we recovered some members of enterobacteriaceae family, producing esbl enzymes, including escherichia coli (67.9%), klebsiella pneumoniae (30.6%), klebsiella oxytoca (0.5%), enterobacter aerogenes (0.5%), and citrobacter freundii (0.5%). β ... | 2014 | 24701189 |
| adjunctive management of central line-associated bloodstream infections with 70% ethanol-lock therapy. | ethanol is bactericidal against most pathogens implicated in central line-associated bloodstream infections (clabsis) and biofilms. current infectious diseases society of america guidelines cite insufficient evidence to support adjunctive ethanol-lock therapy (elt) for central venous catheter (cvc) salvage in patients with clabsi in combination with systemic antimicrobial treatment. we evaluated the safety and potential efficacy of 70% elt for clabsi at our institution after implementation of a ... | 2014 | 24526514 |
| structural and functional insights into peptidoglycan access for the lytic amidase lyta of streptococcus pneumoniae. | the cytosolic n-acetylmuramoyl-l-alanine amidase lyta protein of streptococcus pneumoniae, which is released by bacterial lysis, associates with the cell wall via its choline-binding motif. during exponential growth, lyta accesses its peptidoglycan substrate to cause lysis only when nascent peptidoglycan synthesis is stalled by nutrient starvation or β-lactam antibiotics. here we present three-dimensional structures of lyta and establish the requirements for substrate binding and catalytic activ ... | 2014 | 24520066 |
| isolation of cronobacter spp. (enterobacter sakazakii) from artisanal mozzarella. | cronobacter spp. (enterobacter sakazakii) is an opportunistic bacterial pathogen capable of causing disease and even fatalities in newborn infants within the first weeks of life if consumed as part of the diet. premature and immunocompromised newborn infants are at particular risk. the microorganism has been isolated from a variety of foods including contaminated infant milk formula powder and milk powder substitute. the study aimed to evaluate the level of microbiological contamination in 47 sa ... | 2014 | 27800308 |
| laser optical sensor, a label-free on-plate salmonella enterica colony detection tool. | we investigated the application capabilities of a laser optical sensor, bardot (bacterial rapid detection using optical scatter technology) to generate differentiating scatter patterns for the 20 most frequently reported serovars of salmonella enterica. initially, the study tested the classification ability of bardot by using six salmonella serovars grown on brain heart infusion, brilliant green, xylose lysine deoxycholate, and xylose lysine tergitol 4 (xlt4) agar plates. highly accurate discrim ... | 2014 | 24496794 |
| ndm-1 metallo-β-lactamase and arma 16s rrna methylase producing providencia rettgeri clinical isolates in nepal. | drug-resistant providencia rettgeri producing metallo-β-lactamase and 16s rrna methylase has been reported in several countries. we analyzed p. rettgeri clinical isolates with resistance to carbapenems and aminoglycosides in a hospital in nepal. | 2014 | 24484534 |