Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| klebsiella pneumoniae population genomics and antimicrobial-resistant clones. | antimicrobial-resistant klebsiella pneumoniae (kp) has emerged as a major global public health problem. while resistance can occur across a broad range of kp clones, a small number have become globally distributed and commonly cause outbreaks in hospital settings. here we describe recent comparative genomics investigations that have shed light on kp population structure and the evolution of antimicrobial-resistant clones. these studies provide the basic framework within which genomic epidemiolog ... | 2016 | 27742466 |
| international nosocomial infection control consortium report, data summary of 50 countries for 2010-2015: device-associated module. | we report the results of international nosocomial infection control consortium (inicc) surveillance study from january 2010-december 2015 in 703 intensive care units (icus) in latin america, europe, eastern mediterranean, southeast asia, and western pacific. | 2016 | 27742143 |
| tigecycline-amikacin combination effectively suppresses the selection of resistance in clinical isolates of kpc-producing klebsiella pneumoniae. | by far, only tigecycline, colistin, and some aminoglycosides still show favorable in vitro activities against carbapenem-resistant enterobacteriaceae. however, rapid emergence of resistance often occurs during long-term treatment in clinic, challenging these last resort antimicrobials. in this study, we measured mutant prevention concentration (mpc) and mutant selection window (msw) of tigecycline, colistin and amikacin alone and in combination for clinical isolates of kpc-producing k. pneumonia ... | 2016 | 27594855 |
| patients with culture negative pyogenic liver abscess have the same outcomes compared to those with klebsiella pneumoniae pyogenic liver abscess. | etiologic organism is not frequently isolated despite multiple blood and fluid cultures during management of pyogenic liver abscess (pla). such culture negative pyogenic liver abscess (cnpla) is routinely managed by antibiotics targeted to klebsiella pneumoniae. in this study, we evaluated the outcomes of such clinical practice. | 2016 | 27733320 |
| effects of scutellaria baicalensis on activity and biofilm formation of klebsiella pneumoniae(△). | <strong>objective</strong> to explore the effects of scutellaria baicalensis on activity and biofilm formation of klebsiella pneumonia (kp).<strong>methods</strong> the broth and agar dilution methods were carried out to determine minimum inhibitory concentration and minimum bactericidal concentration of scutellaria baicalensis for tw518. vitek-32 system was used to assay tw518 susceptibility to antibiotics. kp biofilms were formed in vitro and stained with baclight live/dead stain. the class in ... | 2016 | 27733226 |
| nosocomial outbreak of kpc-2- and ndm-1-producing klebsiella pneumoniae in a neonatal ward: a retrospective study. | the spread of resistance to carbapenems among enterobacteriaceae has become a major public health problem in recent years. in this study, we describe an outbreak of klebsiella pneumoniae in the neonatal ward. first, we aimed to study the drug resistance, genetic relatedness, and transmission mechanism of carbapenem-resistant k. pneumoniae; second, we implemented infection control measures to contain the outbreak. | 2016 | 27733128 |
| molecular detection of carbapenemase-producing genes in referral enterobacteriaceae in south africa: a short report. | molecular confirmation of carbapenemase-producing enterobacteriaceae (cpe) was introduced in south africa (sa) at the end of 2011. we report on the detection of these resistance genes based on referral isolates. enterobacteriaceae with non-susceptibility to any of the carbapenems according to defined criteria for antimicrobial susceptibility testing results were sent to a reference laboratory. a proportion of isolates had limited demographic, epidemiological and clinical data available. organism ... | 2016 | 27725012 |
| draft genome sequence of a ctx-m-15-producing klebsiella pneumoniae sequence type 340 (clonal complex 258) isolate from a food-producing animal. | klebsiella pneumoniae carrying blactx-m-15 have been widely disseminated in hospital settings. in this regard, most clinically important strains belong to clonal complex 28 (cc258), which includes sequence type 340 (st340). in this study, we present the draft genome sequence of a ctx-m-15-producing st340 k. pneumoniae strain isolated from a food-producing animal in brazil. | 2016 | 27664870 |
| draft genome sequence of a klebsiella pneumoniae strain (new sequence type 2357) carrying tn3926. | we present the draft genome sequence of a klebsiella pneumoniae carbapenemase-producing sequence type 2357 (st2357) strain, nb60, which contains drug-resistant genes encoding resistance to beta-lactams, fluoroquinolones, aminoglycosides, trimethoprim-sulfamethoxazole, colistin, macrolides, and tetracycline. strain nb60 was isolated from human blood, making it an important tool for studying k. pneumoniae pathogenesis. | 2016 | 27660779 |
| draft genome sequence of klebsiella pneumoniae strain as isolated from zhejiang provincial hospital of tcm, china. | klebsiella pneumoniae is a gram-negative, nonmotile, encapsulated, lactose-fermenting, facultative anaerobic, rod-shaped bacterium. here we present draft genome assemblies of klebsiella pneumoniae as, which was isolated in china. the genomic information will provide a better understanding of the physiology, adaptation, and evolution of k. pneumoniae. | 2016 | 27660770 |
| polymyxin b resistance in carbapenem-resistant klebsiella pneumoniae, são paulo, brazil. | 2016 | 27648951 | |
| outbreak of oxa-48-producing klebsiella pneumoniae involving a sequence type 101 clone in batna university hospital, algeria. | seven nonredundant ertapenem-resistant klebsiella pneumoniae isolates were collected between may 2014 and 19 january 2015 in the nephrology and hematology units of batna university hospital in algeria. all strains coproduced the blaoxa-48, blactx-m-15, blashv-1, and blatem-1d genes. six of these isolates belonged to the pandemic clone sequence type 101 (st101). the blaoxa-48 gene was located on a conjugative incl/m-type plasmid. this is the first known outbreak of oxa-48-producing k. pneumoniae ... | 2016 | 27645236 |
| dissemination of imp-4-encoding pimp-hz1-related plasmids among klebsiella pneumoniae and pseudomonas aeruginosa in a chinese teaching hospital. | a total of 26 blaimp-4-carrying strains of pseudomonas aeruginosa and klebsiella pneumoniae were isolated from 2009 to 2013 in a chinese teaching hospital, and these strains can be assigned into multiple sequence types or allelic profiles as determined by multilocus sequence typing. of these strains, p. aeruginosa p378 and k. pneumoniae 1220 harbor the imp-4-encoding plasmids pp378-imp and p1220-imp, respectively, whose complete nucleotide sequences are determined to be genetically closely relat ... | 2016 | 27641711 |
| crp-cyclic amp regulates the expression of type 3 fimbriae via cyclic di-gmp in klebsiella pneumoniae. | klebsiella pneumoniae is the predominant pathogen isolated from liver abscesses of diabetic patients in asian countries. however, the effects of elevated blood glucose levels on the virulence of this pathogen remain largely unknown. type 3 fimbriae, encoded by the mrkabcdf genes, are important virulence factors in k. pneumoniae pathogenesis. in this study, the effects of exogenous glucose and the intracellular cyclic amp (camp) signaling pathway on type 3 fimbriae expression regulation were inve ... | 2016 | 27631471 |
| microbiology of non-tunnelled catheter-related infections. | aerobic bacterial infections often complicate vascular access in patients receiving haemodialysis, leading to catheter-related blood stream infections (crbsi). various studies report gram - positive bacteria, staphylococcus aureus (s. aureus) in particular, as the most common aetiologic agent. studies on microbiological analysis in this subset of population from india are very few. | 2016 | 27630843 |
| production of xylonic acid by klebsiella pneumoniae. | the glucose oxidation pathway is important for glucose catabolism in klebsiella pneumoniae. gluconic acid and 2-ketogluconic acid are intermediates of this pathway, and the production of these two chemicals has been developed in k. pneumoniae mutants. catalysis characteristic research in this study has shown that xylose is a suitable substrate of the glucose dehydrogenase of this pathway. here, using xylose as substrate, xylonic acid was accumulated in the broth of k. pneumoniae culture, and thi ... | 2016 | 27629123 |
| clinical outcomes, drug toxicity, and emergence of ceftazidime-avibactam resistance among patients treated for carbapenem-resistant enterobacteriaceae infections. | thirty-seven carbapenem-resistant enterobacteriaceae (cre)-infected patients were treated with ceftazidime-avibactam. clinical success and survival rates at 30 days were 59% (22/37) and 76% (28/37), respectively. in 23% (5/22) of clinical successes, cre infections recurred within 90 days. microbiologic failure rate was 27% (10/37). ceftazidime-avibactam resistance was detected in 30% (3/10) of microbiologic failures. | 2016 | 27624958 |
| klebsiella pneumoniae siderophores induce inflammation, bacterial dissemination, and hif-1α stabilization during pneumonia. | klebsiella pneumoniae is a gram-negative pathogen responsible for a wide range of infections, including pneumonia and bacteremia, and is rapidly acquiring antibiotic resistance. k. pneumoniae requires secretion of siderophores, low-molecular-weight, high-affinity iron chelators, for bacterial replication and full virulence. the specific combination of siderophores secreted by k. pneumoniae during infection can impact tissue localization, systemic dissemination, and host survival. however, the ef ... | 2016 | 27624128 |
| molecular epidemiology of kpc-producing klebsiella pneumoniae from invasive infections in italy: increasing diversity with predominance of the st512 clade ii sublineage. | the spread of carbapenem-resistant enterobacteriaceae (cre) represents one of the most worrisome problems for clinical medicine worldwide. in italy, the antibiotic-resistance-istituto superiore di sanità surveillance network, in collaboration with the committee for antimicrobial agents of the italian society of clinical microbiologists, promoted a study to investigate the carbapenem-resistance mechanisms, clonal relatedness and capsular typing of a recent collection of carbapenem-resistant klebs ... | 2016 | 27585968 |
| high minimum inhibitory concentration of imipenem as a predictor of fatal outcome in patients with carbapenem non-susceptible klebsiella pneumoniae. | carbapenem resistance in klebsiella pneumoniae is important because of its increasing prevalence and limited therapeutic options. to investigate the clinical and microbiological characteristics of patients infected or colonized with carbapenem non-susceptible k. pneumoniae (cnskp) in taiwan, we conducted a retrospective study at taipei veterans general hospital from january 2012 to november 2013. carbapenem non-susceptibility was defined as a minimum inhibitory concentration (mic) of ≥2 mg/l for ... | 2016 | 27585787 |
| antimicrobial susceptibility and molecular epidemiology of multidrug-resistant klebsiella pneumoniae in central china. | extended-spectrum-beta-lactamase-producing and carbapenemase-producing klebsiella pneumoniae has rapidly spread through clinical units worldwide. this study investigated the epidemiology and resistance profiles of k. pneumoniae strains isolated in central china between 2009 and 2014. antimicrobial susceptibility tests and polymerase chain reaction were used to investigate the prevalence and resistance of esbls and carbapenemases produced by k. pneumoniae, and the prevalence of kpc-producing k. p ... | 2016 | 27580572 |
| emergence of multidrug-resistant new delhi metallo-β-lactamase-1-producing klebsiella pneumoniae in egypt. | despite expansion of the new delhi metallo-β-lactamase-1 (ndm-1) worldwide, the incident of outbreaks regarding egypt is still uncommon. in this survey, we denounce the emanation of multidrug-resistant ndm-1-producing klebsiella pneumoniae in egypt. we have reclaimed 46 unrepeatable carbapenem-resistant k. pneumoniae isolates at el-demerdash hospital, ain shams university, cairo, egypt. all the isolates showed a decreased sensitivity to imipenem and meropenem via the disc diffusion method. among ... | 2016 | 27575913 |
| plasmid-mediated mcr-1 gene in colistin-resistant clinical isolates of klebsiella pneumoniae in france and laos. | 2016 | 27572402 | |
| enhanced promoter activity by replenishment of sigma factor rpoe in klebsiella pneumoniae. | plasmid-dependent overexpression of enzyme(s) aims to divert carbon flux toward a desired compound. one drawback of this strategy is compromise of growth due to massive consumption of host resources. here we show that replenishment of sigma factor rpoe improves the growth of klebsiella pneumoniae. the gene rpoe was expressed alone or coexpressed with ald4 (an aldehyde dehydrogenase from saccharomyces cerevisiae) in k. pneumoniae. we found that the ald4 activity was higher in the strain coexpress ... | 2016 | 27570311 |
| an outbreak of infections caused by a klebsiella pneumoniae st11 clone coproducing klebsiella pneumoniae carbapenemase-2 and rmtb in a chinese teaching hospital. | klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae bacteria, which cause serious disease outbreaks worldwide, was rarely detected in xiangya hospital, prior to an outbreak that occurred from august 4, 2014, to march 17, 2015. the aim of this study was to analyze the epidemiology and molecular characteristics of the k. pneumoniae strains isolated during the outbreak. | 2016 | 27569227 |
| clinical features and molecular epidemiology of plasmid-mediated dha-type ampc β-lactamase-producing klebsiella pneumoniae blood culture isolates, hong kong. | knowledge of risk factors and clinical characteristics of bacteraemia caused by plasmid-mediated ampc β-lactamase (pampc)-producing klebsiella pneumoniae (pampc-kp) is not well described. this was a retrospective cohort study of patients with k. pneumoniae bacteraemia in three hong kong regional hospitals. demographic and clinical data were retrieved from medical records. pulsed-field gel electrophoresis (pfge) and multilocus sequence typing (mlst) were performed for molecular epidemiology. a to ... | 2016 | 27568104 |
| effect in virulence of switching conserved homologous capsular polysaccharide genes from klebsiella pneumoniae serotype k1 into k20. | the capsular polysaccharides in different serotypes of klebsiella pneumoniae (kp) coded by the (cps) gene cluster are characterized by a conserved and a hyper-variable region. we performed a virulence study by switching genes in the highly conserved region of the cps cluster between strains. six genes in the cps conserved region in serotype k20, including galf, acidppc, wzi, wza, wzb and wzc, were knocked out and replaced by the homologous genes from serotype k1. compared to the parental k20 str ... | 2016 | 27565716 |
| rare case of polymicrobial keratitis with balantidium coli. | to report a rare case of polymicrobial keratitis due to balantidium coli and gram-negative bacteria, pseudomonas aeruginosa and klebsiella pneumoniae, in a soft contact lens (cl) wearer. | 2016 | 27560034 |
| potential transfer of extended spectrum β-lactamase encoding gene, blashv18 gene, between klebsiella pneumoniae in raw foods. | this study investigated the transfer frequency of the extended-spectrum β-lactamase-encoding gene (blashv18) among klebsiella pneumoniae in tryptic soy broth (tsb), pasteurized milk, unpasteurized milk, alfalfa sprouts and chopped lettuce at defined temperatures. all transconjugants were characterized phenotypically and genotypically. kp04(δkm) and kp08(δkm) isolated from seed sprouts and kp342 were used as recipients in mating experiments with k. pneumoniae atcc 700603 serving as the donor. in ... | 2016 | 27554144 |
| mgrb variants in colistin-susceptible and colistin-resistant klebsiella pneumoniae st258. | resistance determinants of a colistin susceptible and five colistin resistant klebsiella pneumoniae st258 from a hungarian outbreak were investigated. a novel mgrb variant in colistin susceptible strain was found. elevated phop and arn gene expressions and wild-type pmrb in all colistin resistant k. pneumoniae were detected. all strains lacked mcr-1. | 2016 | 27553451 |
| identification of a capsular variant and characterization of capsular acetylation in klebsiella pneumoniae pla-associated type k57. | klebsiella pneumoniae can cause community-acquired pyogenic liver abscess (pla). capsular polysaccharide (cps) is important for its virulence. among 79 capsular (k) types discovered thus far, k57 is often associated with pla. here, we report the identification of a k57 variant. cps gene locus sequencing revealed differences between the k57 reference strain 4425/51 (ref-k57) and a variant, the pla isolate a1142. while ref-k57 cps contained orf13 encoding a putative acetyltransferase, the insertio ... | 2016 | 27550826 |
| reinfection and relapse of recurrent bacteremia caused by klebsiella pneumoniae in a medical center in taiwan. | to delineate the genetic relatedness of klebsiella pneumoniae causing recurrent bacteremia. | 2016 | 27546104 |
| molecular epidemiology of extensively drug-resistant klebsiella pneumoniae outbreak in wenzhou, southern china. | the emergence of extensively drug-resistant (xdr) klebsiella pneumoniae has become a major challenge worldwide. in this study, we characterized the phenotypes and genetic features of nine xdr k. pneumoniae isolates from an integrated hospital in zhejiang province, china, from september to october 2014. these xdr k. pneumoniae possessed at least five resistance determinants which contribute to highly resistant to β-lactam, β-lactam/inhibitor combinations, aminoglycosides, quinolones, carbapenems, ... | 2016 | 27542822 |
| emergence of klebsiella pneumoniae co-producing oxa-48, ctx-m-15, and arma in greece. | 2016 | 27542333 | |
| the modulation of mir-155 and mir-23a manipulates klebsiella pneumoniae adhesion on human pulmonary epithelial cells via integrin α5β1 signaling. | micro-rnas (mirnas) critically regulate several host defense mechanisms, but their roles in the bacteria-epithelium interplay remain unclear. our results displayed that the expression of mir-155 and mir-23a were down-regulated in k. pneumoniae-infected pulmonary epithelial cells. the elevated bacterial adhesion on a549 cells followed the enhancement of the cellular levels of these two mirnas. meanwhile, a mechanistic study demonstrated that mir-155 promoted integrin α5β1 function and resulted in ... | 2016 | 27534887 |
| esbl-producing strain of hypervirulent klebsiella pneumoniae k2, france. | 2016 | 27532217 | |
| carbapenemase-producing klebsiella pneumoniae in broiler poultry farming in egypt. | this study investigated the occurrence of carbapenem-resistant klebsiella pneumoniae strains in broiler chickens, drinking water and humans working in contact with chickens and identified the carbapenem resistance determinants among isolates from different sources. internal organs and droppings were collected from 100 broilers with signs of respiratory disease at five broiler farms in egypt. additionally, 20 drinking water samples and 49 faecal samples from workers and veterinarians working at t ... | 2016 | 27530998 |
| ceftazidime/avibactam: a novel cephalosporin/nonbeta-lactam beta-lactamase inhibitor for the treatment of complicated urinary tract infections and complicated intra-abdominal infections. | there has been greater interest in developing additional antimicrobial agents due to the increasing health care costs and resistance resulting from bacterial pathogens to currently available treatment options. gram-negative organisms including enterobacteriaceae and pseudomonas aeruginosa are some of the most concerning threats due to their resistance mechanisms: extended-spectrum beta-lactamase production and klebsiella pneumoniae carbapenemase enzymes. ceftazidime is a third-generation broad-s ... | 2016 | 27528799 |
| detection of blakpc and blages carbapenemase genes in klebsiella pneumoniae isolated from hospitalized patients in kashan, iran. | klebsiella pneumoniae carbapenemase (kpc)-producing bacteria are among the highly antimicrobial resistant gram negative bacteria and infections due to them are an increasingly major health problem worldwide. | 2016 | 27527726 |
| alterations in outer membrane permeability favor drug-resistant phenotype of klebsiella pneumoniae. | the increasing number of infections caused by multidrug-resistant and pandrug-resistant bacteria represents a serious worlwide problem. drug resistance limits available antimicrobials and can lead to suboptimal treatment of bacterial infections. it can be predicted that resistance to more antimicrobial drugs will be acquired by even more bacteria in the future. among the distinct resistance strategies, preventing drug entrance to intracellular compartment through modification of membrane permeab ... | 2016 | 27526080 |
| antimicrobial resistance among aerobic biofilm producing bacteria isolated from chronic wounds in the tertiary care hospitals of peshawar, pakistan. | chronic wound infections impose major medical and economic costs on health-care systems, cause significant morbidity, mortality and prolonged hospitalisation. the presence of biofilm producing bacteria in these wounds is considered as an important virulence factor that leads to chronic implications including ulceration. the undertaken study aimed to isolate and identify the biofilm aerobic bacterial pathogens from patients with chronic wound infections, and determine their antibiotics resistance ... | 2016 | 27523661 |
| klebseq, a diagnostic tool for surveillance, detection, and monitoring of klebsiella pneumoniae. | health care-acquired infections (hais) kill tens of thousands of people each year and add significantly to health care costs. multidrug-resistant and epidemic strains are a large proportion of hai agents, and multidrug-resistant strains of klebsiella pneumoniae, a leading hai agent, have caused an urgent public health crisis. in the health care environment, patient colonization by k. pneumoniae precedes infection, and transmission via colonization leads to outbreaks. periodic patient screening f ... | 2016 | 27510832 |
| endotipsitis caused by extremely drug-resistant klebsiella pneumoniae. | 2016 | 27509714 | |
| acinetobacter spp. are associated with a higher mortality in intensive care patients with bacteremia: a survival analysis. | it has been challenging to determine the true clinical impact of acinetobacter spp., due to the predilection of this pathogen to colonize and infect critically ill patients, who often have a poor prognosis. the aim of this study was to assess whether acinetobacter spp. bacteremia is associated with lower survival compared with bacteremia caused by other pathogens in critically ill patients. | 2016 | 27506948 |
| invasive liver abscess syndrome predisposed by klebsiella pneumoniae related prostate abscess in a nondiabetic patient: a case report. | prostate abscess is usually a complication of acute urinary tract infection. invasive liver abscess syndrome is characterized with klebsiella pneumoniae related multiple organ metastasis. concomitant pyogenic liver abscess and prostate abscess have rarely been reported. recurrent episode of liver abscess is even rarer. | 2016 | 27506523 |
| influence of camp receptor protein (crp) on bacterial virulence and transcriptional regulation of alls by crp in klebsiella pneumoniae. | camp receptor protein (crp) is one of the most important transcriptional regulators, which can regulate large quantities of operons in different bacteria. the gene alls was well-known as allantoin-utilizing capability and involving in bacterial virulence in klebsiella pneumoniae (k. pneumoniae). the specific dna recognition motif of transcription regulator crp was found in alls promoter region. therefore, this study is aimed to investigate the function of crp on virulence and its transcriptional ... | 2016 | 27502416 |
| synergistic effects of baicalein with cefotaxime against klebsiella pneumoniae through inhibiting ctx-m-1 gene expression. | generation of extended- spectrum β- lactamases is one of the major mechanisms by which clinical klebsiella pneumoniae develop resistance to antibiotics. combined antibiotics prove to be a relatively effective method of controlling such resistant strains. some of chinese herbal active ingredients are known to have synergistic antibacterial effects. this study is aimed to investigate synergistic effects of chinese herbal active ingredients with cefotaxime on the extended- spectrum β- lactamase pos ... | 2016 | 27502110 |
| association between presence of virulence genes and antibiotic resistance in clinical klebsiella pneumoniae isolates. | to investigate the presence of rmpa and wcag virulence genes and class 1, 2, and 3 integrons, and to evaluate a relationship between antibiotic resistance and virulence in klebsiella pneumoniae methods: we collected a total of 200 k. pneumoniae isolates from hospitals in tehran, iran. antibiotic susceptibility was determined using the disk diffusion method. the extended-spectrum β-lactamase (esbl) producers were detected using the combination disk method. we detected the rmpa and wcag genes and ... | 2016 | 27498999 |
| in vivo efficacy of single phage versus phage cocktail in resolving burn wound infection in balb/c mice. | klebsiella pneumoniae is one of the most predominant pathogens associated with burn wound infections, causing considerable morbidity and mortality. the indiscriminate usage of antibiotics has led to the development of resistant strains, which have contributed towards the inefficacy of antibiotics. phage therapy is a promising alternative to hinder the progression of pathogenic bacteria. however, phage bacterial resistance is already well known but the use of phage cocktails can overcome this dra ... | 2016 | 27498362 |
| successful treatment of urinary tract infection in kidney transplant recipients caused by multiresistant klebsiella pneumoniae producing new delhi metallo-beta-lactamase (ndm-1) with strains genotyping. | klebsiella pneumoniae new delhi metallo-beta-lactamase-1 (ndm-1) strains have recently become a new threat in kidney transplant recipients due to the strains' resistance to almost all antibiotics, including carbapenems. | 2016 | 27496450 |
| oral decontamination with aminoglycosides is associated with lower risk of mortality and infections in high-risk patients colonized with colistin-resistant, kpc-producing klebsiella pneumoniae. | invasive infections caused by kpc-producing klebsiella pneumoniae (kpckp) are associated with very high mortality. because infection is usually preceded by rectal colonization, we investigated if decolonization therapy (dt) with aminoglycosides had a protective effect in selected patients. | 2016 | 27494911 |
| nosocomial outbreak of oxa-48-producing klebsiella pneumoniae in a chinese hospital: clonal transmission of st147 and st383. | in china, the spread and outbreak of oxa-48-producing enterobacteriaceae remains largely unknown. | 2016 | 27490695 |
| klebsiella pneumoniae bloodstream infection, antimicrobial resistance and consumption trends in ireland: 2008 to 2013. | this study aimed to describe the epidemiology and antimicrobial resistance trends of klebsiella pneumoniae bloodstream infection (bsi) in ireland, in conjunction with national antimicrobial consumption data, during the period 2008 to 2013. a retrospective cohort study of k. pneumoniae bsi cases was conducted, based on notifications from irish microbiology laboratories to the health protection surveillance centre (hpsc). in total, 1942 k. pneumoniae bsi cases were identified over 6 years, with 31 ... | 2016 | 27488438 |
| high prevalence of hypervirulent klebsiella pneumoniae infection in china: geographic distribution, clinical characteristics, and antimicrobial resistance. | hypervirulent klebsiella pneumoniae (hvkp) is traditionally defined by hypermucoviscosity, but data based on genetic background are limited. antimicrobial-resistant hvkp has been increasingly reported but has not yet been systematically studied. k. pneumoniae isolates from bloodstream infections, hospital-acquired pneumonia, and intra-abdominal infections were collected from 10 cities in china during february to july 2013. clinical data were collected from medical records. all k. pneumoniae isol ... | 2016 | 27480857 |
| genetic diversity of klebsiella pneumoniae isolates during an outbreak in a non-human primate research colony. | klebsiella pneumoniae can be a serious pathogen in non-human primates, particularly neotropical monkeys. | 2016 | 27477611 |
| bloodstream infections caused by klebsiella pneumoniae carbapenemase 2-producing k. pneumoniae at a hematology department in wenzhou, china. | the increasing prevalence of klebsiella pneumoniae carbapenemase 2-producing k. pneumoniae (kpc-2-kp) infections can become a new life-threatening complication for hematological patients. five cases of kpc-2-kp bloodstream infections have been identified in our hematology department over the past 10 years. the current treatment options do not show satisfactory efficacy, especially for bloodstream infections. the treatment of these five cases was unsuccessful, mainly due to the high minimum inhib ... | 2016 | 27477421 |
| molecular signature of extended spectrum β-lactamase producing klebsiella pneumoniae isolated from bovine milk in eastern and north-eastern india. | the present study reports on 23 extended spectrum β-lactamase producing klebsiella pneumoniae (kp), isolated from milk samples (n=340) of healthy cows (n=129) and cows with subclinical (n=159) and clinical (n=52) mastitis, from three different states of india viz. west bengal, jharkhand and mizoram. seven of them were ampc type β-lactamase producers, as well. the esbl producing kp were significantly (p=0.006, χ2=10.04, df=2) and more frequently detected in milk samples of mastitic cows than heal ... | 2016 | 27473782 |
| carbapenem-resistant enterobacteriaceae in special populations: solid organ transplant recipients, stem cell transplant recipients, and patients with hematologic malignancies. | carbapenem-resistant enterobacteriaceae (cre) are a major global public health concern and pose a serious threat to immunocompromised hosts, particularly patients with hematologic malignancies and solid organ (sot) and stem cell transplant recipients. in endemic areas, carbapenem-resistant klebsiella pneumoniae infections occur in 1-18% of sot recipients, and patients with hematologic malignancies represent 16-24% of all patients with cre bacteremia. mortality rates approaching 60% have been rep ... | 2016 | 27470662 |
| [rapid increase in resistance to third generation cephalosporins, imipenem and co-resistance in klebsiella pneumoniae from isolated from 7,140 blood-cultures (2010-2014) using ears-net data in spain]. | an analysis was made about the evolution of resistance to 3rd generation cephalosporins, imipenem, and other antibiotics in invasive isolates of klebsiella pneumoniae (k. pneumoniae) according to the spanish ears-net database (2010-2014). | 2016 | 27469416 |
| knockdown of hmgn2 increases the internalization of klebsiella pneumoniae by respiratory epithelial cells through the regulation of α5β1 integrin expression. | integrin receptors, a large family of adhesion receptors, are involved in the attachment of klebsiella pneumoniae to respiratory epithelial cells, and subsequently cause the internalization of k. pneumoniae by host cells. although a number of molecules have been reported to regulate the expression and activity of integrin receptors in respiratory epithelial cells, the specific underlying molecular mechanisms remain largely unknown. high mobility group nucleosomal binding domain 2 (hmgn2), a non- ... | 2016 | 27460641 |
| endogenous endophthalmitis in the american and korean population: an 8-year retrospective study. | to study the clinical features of endogenous endophthalmitis (ee) in sample patient populations from the usa and south korea over an 8-year period. | 2016 | 27459423 |
| successful treatment of a disseminated infection with extensively drug-resistant klebsiella pneumoniae in a liver transplant recipient with a fosfomycin-based multidrug regimen. | donor-derived infections with multidrug-resistant gram-negative bacteria are associated with poor outcomes, in part because of limited treatment options. here, we describe a case of donor-derived, disseminated infection with colistin-resistant, carbapenemase-producing klebsiella pneumoniae in a liver transplant recipient that was cured with addition of intravenous fosfomycin to a multidrug regimen, in conjunction with aggressive surgical source control. intravenous fosfomycin represents a promis ... | 2016 | 27458980 |
| performance of four different agar plate methods for rectal swabs, synergy disk tests and metallo-β-lactamase etest for clinical isolates in detecting carbapenemase-producing klebsiella pneumoniae. | the aims of the study were to compare four different agar plate methods in the identification of carbapenemase-producing klebsiella pneumoniae (cp-kp) from rectal samples and to assess the role of phenotypic methodologies in the identification of carbapenemase type from clinical k. pneumoniae isolates. two chromogenic agars (brilliance cre and chromagar kpc) were compared to macconkey agar plates with ertapenem (ert) or imipenem (imp) disks for the identification of cp-kp from 912 rectal swabs. ... | 2016 | 27452448 |
| phenotypic and molecular characterization of antimicrobial resistance in klebsiella spp. isolates from companion animals in japan: clonal dissemination of multidrug-resistant extended-spectrum β-lactamase-producing klebsiella pneumoniae. | the emergence of antimicrobial resistance in klebsiella spp., including resistance to extended-spectrum cephalosporins (esc) and fluoroquinolones, is of great concern in both human and veterinary medicine. in this study, we investigated the prevalence of antimicrobial resistance in a total of 103 klebsiella spp. isolates, consisting of klebsiella pneumoniae complex (kp, n = 89) and k. oxytoca (ko, n = 14) from clinical specimens of dogs and cats in japan. furthermore, we characterized the resist ... | 2016 | 27446056 |
| kpc-3-producing klebsiella pneumoniae in portugal linked to previously circulating non-cg258 lineages and uncommon genetic platforms (tn4401d-incfia and tn4401d-incn). | kpc-3-producing bacteria are endemic in many countries but only recently became apparent their wide distribution in different portuguese hospitals. the aim of this study is to characterize genetic backgrounds associated with bla kpc-3 among klebsiella pneumoniae isolates recently identified on non-hospitalized patients in portugal. twenty kpc-producing k. pneumoniae identified between october 2014 and november 2015 in three different community laboratories were characterized. isolates were mainl ... | 2016 | 27446040 |
| persistent cutaneous abdominal ulcerations secondary to diffuse dermal angiomatosis: an underestimated sign for severe atherosclerosis: a case report. | diffuse dermal angiomatosis (dda) is a rare, acquired, reactive vascular proliferation, clinically characterized by livedoid erythematous-violaceous plaques, which frequently evolve to ulceration and necrosis. histopathologically, it is manifested by a diffuse proliferation of endothelial cells within the full thickness of the dermis. dda has been mainly associated with severe peripheral atherosclerosis. | 2016 | 27442644 |
| adaptive laboratory evolution of klebsiella pneumoniae for improving 2,3-butanediol production. | microbial production of 2,3-butanediol is limited by the toxic components in the lignocellulose hydrolysate. to improve the 2,3-butanediol production via klebsiella pneumoniae from cotton stalk hydrolysate, a method coupling a high tolerance of strain and detoxification of the hydrolysate was thus investigated in this study. the strain tolerance of k. pneumoniae to the cotton stalk hydrolysate was improved via an adaptive laboratory evolution, which involved a stepwise increase in the hydrolysat ... | 2016 | 27442598 |
| epidemiology of carbapenem resistant klebsiella pneumoniae infections in mediterranean countries. | infections by carbapenem-resistant enterobacteriaceae (cre), in particular, carbapenem-resistant klebsiella pneumoniae (crkp), are a significant public health challenge worldwide. resistance to carbapenems in enterobacteriaceae is linked to different mechanisms, including the production of the various types of enzymes like kpc, vim, imp, ndm, and oxa-48. despite several attempts to control the spread of these infections at the local and national level, the epidemiological situation for crkp had ... | 2016 | 27441063 |
| the role of budabc on 1,3-propanediol and 2,3-butanediol production from glycerol in klebsiella pneumoniae cicim b0057. | 1,3-propanediol (1,3-pd) is an important compound from which many others can be synthesized. 2,3-butanediol (bdo) is the key by-product in the biosynthesis of 1,3-pd from glycerol, but it impedes its downstream purification. in klebsiella, the buda, budb and budc genes encode enzymes that are responsible for the synthesis of bdo. in this study, 3 individual antisense rnas were designed to repress the expression and hence activity of buda-c. compared with the parent strains, the activities of bud ... | 2016 | 27439015 |
| klebsiella pneumoniae esbl forming spheroplasts in the fresh and unstained urine sediment. | a 60 year-old man was submitted to kidney transplantation in 2013 due to chronic renal insufficiency caused by hypertension. he had recurrent episodes of urinary tract infection and came to the hospital due to a 4 day-long fever, abdominal pain, burning urination and nausea. routine urinalysis revealed a picture of infection (> 50 leucocytes/high power field associated to massive bacteriuria). the urine sediment revealed elongated like elements with an enlarged part in the middle of the structur ... | 2016 | 27438984 |
| klebsiella pneumoniae infection leads to a poor visual outcome in endogenous endophthalmitis: a 12-year experience in southern taiwan. | to compare the characteristics, visual outcome, and prognostic factors of patients with endogenous endophthalmitis and to determine the association of endophthalmitis with klebsiella pneumoniae infection. | 2016 | 27438458 |
| carriage of multidrug resistant bacteria on frequently contacted surfaces and hands of health care workers. | maximal contact between the patients and health care workers (hcws) happens in the intensive care units (icu). control of nosocomial infections requires compliance with hand hygiene and contamination free surfaces. | 2016 | 27437214 |
| molecular typing and genetic environment of the blakpc gene in chilean isolates of klebsiella pneumoniae. | the aim of this work was to determine the genetic environment and transferability of blakpc as well as the pulsotypes of kpc-producing klebsiella pneumoniae strains isolated from clinical samples in chilean hospitals. seventeen strains, principally isolated in santiago (the capital of chile) during the years 2012 and 2013, were included. the genetic environment of blakpc was elucidated by pcr mapping and sequencing. molecular typing was performed by pulsed-field gel electrophoresis (pfge) and mu ... | 2016 | 27436389 |
| spotlight on ceftazidime/avibactam: a new option for mdr gram-negative infections. | during the last decade infections caused by mdr gram-negative bacteria (gnb) have become increasingly prevalent. because of their high morbidity and mortality rates, these infections constitute a serious threat to public health worldwide. ceftazidime/avibactam is a new approved agent combining ceftazidime and a novel β-lactamase inhibitor with activity against various β-lactamases produced by mdr gnb. avibactam has a spectrum of inhibition of class a and c β-lactamases, including esbls, ampc and ... | 2016 | 27432599 |
| immunotherapies against antibiotics-resistant klebsiella pneumoniae. | 2016 | 27431874 | |
| emergence and plasmid analysis of klebsiella pneumoniae kp01 carrying blages-5 from guangzhou, china. | klebsiella pneumoniae strain kp01 carrying blages-5 was identified from a patient in guangzhou, china. high-throughput sequencing assigned blages-5 to a 28.5-kb nonconjugative plasmid, pges-gz. a 13-kb plasmid backbone sequence on pges-gz was found to share high sequence identities with plasmids from gram-negative nonfermenters. a novel class 1 integron carrying a gene cassette array of orf28-orf28-blages-5 was identified on pges-gz, within which orf28 encoded a hypothetical protein possibly cor ... | 2016 | 27431225 |
| experimental single-strain mobilomics reveals events that shape pathogen emergence. | virulence genes on mobile dnas such as genomic islands (gis) and plasmids promote bacterial pathogen emergence. excision is an early step in gi mobilization, producing a circular gi and a deletion site in the chromosome; circular forms are also known for some bacterial insertion sequences (iss). the recombinant sequence at the junctions of such circles and deletions can be detected sensitively in high-throughput sequencing data, using new computational methods that enable empirical discovery of ... | 2016 | 27378783 |
| efficient transmission of incfiiy and incl plasmids and klebsiella pneumoniae st101 clone producing oxa-48, ndm-1 or oxa-181 in bucharest hospitals. | 2016 | 27378198 | |
| lactobacillus salivarius reverse diabetes-induced intestinal defense impairment in mice through non-defensin protein. | altered intestinal microbiota and subsequent endotoxemia play pathogenic roles in diabetes. we aimed to study the mechanisms of intestinal defense impairment in type 1 diabetes and the effects of lactobacillus salivarius as well as fructooligosaccharides (fos) supplementation on diabetes-induced bacterial translocation. alterations in the enteric microbiome, expression of mucosal antibacterial proteins and bacteria-killing activity of the intestinal mucosa in streptozotocin (stz)-induced diabeti ... | 2016 | 27376728 |
| decontamination of indoor air to reduce the risk of airborne infections: studies on survival and inactivation of airborne pathogens using an aerobiology chamber. | although indoor air can spread many pathogens, information on the airborne survival and inactivation of such pathogens remains sparse. | 2016 | 27375064 |
| countrywide dissemination of a dha-1-type plasmid-mediated ampc β-lactamase-producing klebsiella pneumoniae st11 international high-risk clone in hungary, 2009-2013. | the first plasmid-mediated ampc β-lactamase-producing klebsiella pneumoniae (pampc kp) isolate was detected in december 2009 in hungary. hungarian microbiological laboratories were asked to send all kp strains showing cefoxitin resistance and decreased susceptibility or resistance to any third-generation cephalosporins to the reference laboratories at the national center for epidemiology. investigation was conducted in order to outline spatio-temporal distribution and genetic characterization of ... | 2016 | 27375036 |
| [impact of extended-spectrum β-lactamase on clinical outcome and medical cost in patients with bloodstream infection due to klebsiella pneumoniae]. | to evaluate the impact of extended-spectrum β-lactamase (esbl) on clinical outcome and medical cost in patients with bloodstream infection (bsi) due to klebsiella pneumoniae. | 2016 | 27373357 |
| risk factors for infections due to carbapenem-resistant klebsiella pneumoniae after open heart surgery. | patients undergoing major surgery are at increased risk of developing infections due to resistant organisms, including carbapenem-resistant klebsiella pneumoniae (cr-kp). in this study, we assessed risk factors for cr-kp infections after open heart surgery in a teaching hospital in northern italy. | 2016 | 27371609 |
| boronic acid disk diffusion for the phenotypic detection of polymerase chain reaction-confirmed, carbapenem-resistant, gram-negative bacilli isolates. | the middle east is regarded as a secondary reservoir for oxa-48 and new delhi metallo-β-lactamase (ndm) carbapenemases. one of the main challenges in clinical microbiology diagnostics is the detection of carbapenemases. for this reason simple screening methods have been sought to detect carbapenemase producers to determine appropriate therapeutic measures and implement infection control interventions. the present study aimed to evaluate the efficacy of the modified hodge test (mht) and a boronic ... | 2016 | 27368987 |
| antibacterial constituents of fructus chebulae immaturus and their mechanisms of action. | to extract, purify, and identify the effective constituents of aqueous extract of fructus chebulae immaturus, and analyze the bactericidal effects of total tannins. | 2016 | 27368700 |
| complement resistance mechanisms of klebsiella pneumoniae. | the current emergence of antibiotic-resistant bacteria causes major problems in hospitals worldwide. to survive within the host, bacterial pathogens exploit several escape mechanisms to prevent detection and killing by the immune system. as a major player in immune defense, the complement system recognizes and destroys bacteria via different effector mechanisms. the complement system can label bacteria for phagocytosis or directly kill gram-negative bacteria via insertion of a pore-forming compl ... | 2016 | 27364766 |
| rise and fall of kpc-producing klebsiella pneumoniae in new york city. | the study objective was to examine the epidemiological trends of kpc-producing klebsiella pneumoniae in new york city medical centres. | 2016 | 27353464 |
| [distribution of pathogenic bacteria and its influence on expression of bcl-2 and bax protein after hsct in the patients with hematological malignancies]. | to investigate the distribution of pathogenic bacteria in the patients with hematologic malignancies received hematopoietic stem cell transplantation (hsct) and its influence on the expression of bcl-2 and bax proteins. | 2016 | 27342520 |
| deletion of ldha and aldh genes in klebsiella pneumoniae to enhance 1,3-propanediol production. | to improve 1,3-propanediol (1,3-pd) production and reduce byproduct concentration during the fermentation of klebsiella pneumonia. | 2016 | 27341834 |
| complete genome sequence of the first kpc-type carbapenemase-positive proteus mirabilis strain from a bloodstream infection. | sequencing of the blakpc-positive strain proteus mirabilis aouc-001 was performed using both the miseq and pacbio rs ii platforms and yielded a single molecule of 4,272,433 bp, representing the complete chromosome. genome analysis showed the presence of several acquired resistance determinants, including two copies of blakpc-2 carried on a fragment of a kpc-producing plasmid previously described in klebsiella pneumoniae. | 2016 | 27340072 |
| gluconic acid production by gad mutant of klebsiella pneumoniae. | klebsiella pneumoniae produces many economically important chemicals. using glucose as a carbon source, the main metabolic product in k. pneumoniae is 2,3-butanediol. gluconic acid is an intermediate of the glucose oxidation pathway. in the current study, a metabolic engineering strategy was used to develop a gluconic acid-producing k. pneumoniae strain. deletion of gad, resulting in loss of gluconate dehydrogenase activity, led to the accumulation of gluconic acid in the culture broth. gluconic ... | 2016 | 27339313 |
| lactobacillus casei triggers a tlr mediated rack-1 dependent p38 mapk pathway in caenorhabditis elegans to resist klebsiella pneumoniae infection. | in the present study, the effect of lactic acid bacteria (lab) was investigated at the molecular level using the model organism caenorhabditis elegans against klebsiella pneumoniae. out of the 13 lab screened, lactobacillus casei displayed excellent protective efficacy by prolonging the survival of k. pneumoniae-infected nematodes. pretreatment with l. casei significantly decreased bacterial colonization and rescued k. pneumoniae-infected c. elegans from various physiological impairments. the co ... | 2016 | 27338631 |
| mobilization of carbapenemase-mediated resistance in enterobacteriaceae. | there has been a dramatic increase in the last decade in the number of carbapenem-resistant enterobacteriaceae, often leaving patients and their providers with few treatment options and resultant poor outcomes when an infection develops. the majority of the carbapenem resistance is mediated by bacterial acquisition of one of three carbapenemases (klebsiella pneumoniae carbapenemase [kpc], oxacillinase-48-like [oxa-48], and the new delhi metallo-β-lactamase [ndm]). each of these enzymes has a uni ... | 2016 | 27337454 |
| evaluation of antibacterial activities of colistin, rifampicin and meropenem combinations against ndm-1-producing klebsiella pneumoniae in 24 h in vitro time-kill experiments. | to investigate the activity of colistin alone or in double and triple combination with rifampicin and meropenem against ndm-1-producing klebsiella pneumoniae. | 2016 | 27334665 |
| first report of a clinical isolate of new delhi metallo-β-lactamase-producing klebsiella pneumoniae in brazil. | 2016 | 27328614 | |
| emergence and characterization of tigecycline resistance in multidrug-resistant klebsiella pneumoniae isolates from blood samples of patients in intensive care units in northern china. | serious infections in intensive care unit patients caused by multidrug-resistant (mdr) klebsiella pneumoniae represent a major threat worldwide owing to increased mortality and limited treatment options. with the application of tigecycline for mdr pathogens, tigecycline-non-susceptible k. pneumoniae isolates have recently emerged in china. to identify the susceptibility profile of mdr k. pneumoniae to tigecycline and evaluate the molecular characterization of tigecycline resistance, 214 mdr k. p ... | 2016 | 27324378 |
| biofilm inhibitory effect of chlorhexidine conjugated gold nanoparticles against klebsiella pneumoniae. | klebsiella pneumoniae (k. pneumoniae) is one of the major pathogen associated with nosocomial infections, especially catheter associated urinary tract infections which involved biofilm formation. this study was designed to evaluate the antibiofilm efficacy of gold nanoparticle conjugated with chlorhexidine (au-chx) against k. pneumoniae isolates. au-chx was synthesized and analyzed for stability by using uv-visible spectrophotometry, atomic force microscopy (afm), fourier transform infrared spec ... | 2016 | 27321770 |
| solar photocatalysis as disinfection technique: inactivation of klebsiella pneumoniae in sewage and investigation of changes in antibiotic resistance profile. | the presence of pathogenic microorganisms in wastewater and their resistant nature to antibiotics impose effective disinfection treatment for public health and environmental protection. in this work, photocatalysis with metal-doped titania under artificial and natural sunlight, chlorination and uv-c irradiation were evaluated for their potential to inactivate klebsiella pneumoniae in real wastewater. their overall effect on antibiotic resistance profile and target antibiotic resistance genes (ar ... | 2016 | 27316624 |
| comparison of six commercial kits to extract bacterial chromosome and plasmid dna for miseq sequencing. | we compared commercial kits for extraction of genomic dna from the gram-negative bacterium klebsiella pneumoniae for subsequent miseq sequencing. purification of dna was based on matrix binding (silica or anion exchange resin) or differential precipitation (salting out), respectively. the choice of extraction kit had little effect on sequencing quality and coverage across drastically different replicons, except for an apparent depletion of small plasmids (<5 kb) during precipitation-based extrac ... | 2016 | 27312200 |
| klebsiella pneumoniae: going on the offense with a strong defense. | klebsiella pneumoniae causes a wide range of infections, including pneumonias, urinary tract infections, bacteremias, and liver abscesses. historically, k. pneumoniae has caused serious infection primarily in immunocompromised individuals, but the recent emergence and spread of hypervirulent strains have broadened the number of people susceptible to infections to include those who are healthy and immunosufficient. furthermore, k. pneumoniae strains have become increasingly resistant to antibioti ... | 2016 | 27307579 |