Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| functional mapping of the epstein-barr virus genome: identification of sites coding for the restricted early antigen, the diffuse early antigen, and the nuclear antigen. | attempts were made to functionally map antigenic expression of the epstein-barr virus (ebv) to specific regions on the ebv genome, using the b95-8 strain. experiments were performed to map the expression of early antigen (ea), both restricted and diffuse (r and d, respectively), and the ebv nuclear antigen (ebna), using intact b95-8 dna, cloned bamhi fragments or charon 4a fragments. dna preparations were microinjected into two ebv genome-negative epithelial tumor cell lines. expression of ebv a ... | 1983 | 6310877 |
| identification of a region of the epstein-barr virus (b95-8) genome required for transformation. | in a previous study the identification of a region(s) of the epstein-barr virus (ebv) genome, which is associated with transformation, was attempted by marker rescue. a transforming ebv was rescued from d98/hr-1 hybrid cells, which contain the non-transforming hr-1 ebv genome, after transfection with specific bamhi and charon 4a fragments (j. stoerker and r. glaser, proc. nat. acad. sci. usa 80, 1726-1729, 1983). in this study, characterization of the ebv dna in four human lymphoblastoid cell li ... | 1983 | 6310878 |
| [hepatitis syndrome following heart surgery using extracorporeal circulation]. | 195 patients who underwent heart operations by means of extra-corporal circulation were followed-up for virus hepatitis in a prospective study. 15 cases of hepatitis occurred, 6 developed hepatitis type b, 9 hepatitis non-a-non-b. 41 out of 142 patients examined had no antibodies against cytomegaly virus, in 7 patients a seroconversion took place. epstein-barr-virus does not play a clinical role. | 1983 | 6310912 |
| acquired immunodeficiency syndrome in infants. | 1983 | 6310988 | |
| epstein-barr virus (ebv) induced polyclonal and monoclonal b-cell lymphoproliferative diseases occurring after renal transplantation. clinical, pathologic, and virologic findings and implications for therapy. | nineteen renal allograft recipients developed b-cell lymphoproliferative diseases. clinically there were two groups: a) young patients (mean age, 23 years) who presented soon (mean, 9 months) after transplantation or antirejection therapy with fever, pharyngitis, and lymphadenopathy resembling infectious mononucleosis, and b) older patients (mean age, 48 years) who presented later (mean, 6 years) after transplantation with localized tumor masses. histologically, the diseases were classified as p ... | 1983 | 6311121 |
| studies of the epstein-barr herpesvirus relationship with hodgkin's disease and the use of virus immunological markers in differential diagnostics of nasopharyngeal carcinoma. | 1983 | 6311138 | |
| on epstein-barr virus (ebv) infection in greenland eskimos, danes in greenland, and danes in denmark. | 1983 | 6311139 | |
| the significance of serologic test for eb-virus antibody in occult type nasopharyngeal carcinoma. | since antibody titers against epstein-barr virus (ebv) antigens are often elevated in sera of patient with active undifferentiated and nonkeratinizing nasopharyngeal carcinoma, anti-ebv serologic tests have been applied for diagnosis of metastatic lymphadenopathy in the neck without an obvious source and/or suspicious tumor of the nasopharynx. in this study it is emphasized that the serologic test procedure was helpful in diagnosing a patient with nasopharyngeal carcinoma that was initially occu ... | 1983 | 6311149 |
| human alpha-interferon enhances in vitro igm rheumatoid factor synthesis by lymphocytes from normal subjects and rheumatoid arthritis patients. | alpha-interferon (alpha-ifn) was added to pokeweed mitogen (pwm) or epstein-barr virus stimulated human peripheral blood mononuclear cell cultures from normal subjects or patients with rheumatoid arthritis (ra). alpha-ifn enhanced in vitro production of pwm induced igg and igm, and significantly enhanced pwm induced igm rheumatoid factor (igm-rf) production by lymphocytes both from normal subjects and ra patients. enhancement was recorded whether cells were preincubated with alpha-ifn for 16 hou ... | 1983 | 6311224 |
| serum anti-ebv iga is inversely related to tumor infiltration by surface iga positive lymphocytes in nasopharyngeal carcinoma. | the mean number of tumor-infiltrating lymphoid cells with surface iga was moderately higher in primary nasopharyngeal carcinoma (npc) than in their neck gland metastases or in other head and neck tumors. within the group of primary npc, patients with large numbers of iga positive infiltrating cells had a significantly lower mean iga antibody titer to epstein-barr viral capsid antigen. it is unlikely therefore that the unique, frequent elevation of this antibody titer in npc is due to stimulation ... | 1983 | 6311307 |
| new method for detecting epstein-barr virus association in nonproducer lymphoblastoid cell lines. | epstein-barr virus association in nonproducer human lymphoblastoid cell lines can be demonstrated by the presence of the virus genome (nucleic acid hybridization studies) or by the detection of the virus-coded complement-fixing antigen (complement fixation and/or anti-complement immunofluorescent test). this paper describes an enzyme immunoassay for the detection of epstein-barr virus complement-fixing antigen and its application to the demonstration of epstein-barr virus association in nonprodu ... | 1983 | 6311404 |
| increased sensitivity to natural killing in raji cells is due to effector recognition of molecules appearing on target cell membranes following ebv cycle induction. | previous studies demonstrated that nk resistant epstein-barr virus (ebv) carrying human lymphoblastoid cell lines become sensitive to nk cell-mediated destruction following induction of the viral cycle by superinfection with the p3hr-1 substrain of ebv or chemicals. in the present report we analysed the cellular membrane changes that were related to the development of sensitivity to nk activity in raji cells with metabolic inhibitors. nk sensitivity does not develop in p3hr-1 superinfected raji ... | 1983 | 6311470 |
| rheumatoid arthritis. | 1983 | 6311471 | |
| [serological and biochemical studies in late stage of nasopharyngeal carcinoma]. | serological and biochemical studies were done at the national taiwan university hospital, on 243 patients with biopsy-proved anaplastic nasopharyngeal carcinoma (npc) in various clinical conditions. the remarkable elevation of both igg and iga antibody titers against eb virus was specific for npc patients. the seropositive rates ranged from 75% to 100% in various stages of npc patients. serum igg and iga levels were also increased moderately and nonspecifically in stages ii-iv and recurrent pati ... | 1983 | 6311498 |
| the clinical use of intravenous acyclovir. | acyclovir (acycloguanosine) is a new antiviral compound with activity against certain herpes viruses. acyclovir is phosphorylated preferentially in virus-infected cells into its active form, acyclovir triphosphate, an inhibitor of viral-induced dna polymerase. acyclovir, which possesses an acyclic carbohydrate moiety, also causes premature dna chain termination. acyclovir has shown clinical activity against herpes simplex virus (hsv) types 1 and 2 and varicella zoster virus (vzv), but its useful ... | 1983 | 6311503 |
| evidence that the alpha 2hs glycoprotein receptor and the epstein-barr virus nuclear antigen (ebna) are identical. | evidence is presented which suggests that the alpha 2hs glycoprotein (alpha 2hs) receptor on lymphocytes transformed by epstein-barr virus (ebv) and the ebv-determined nuclear antigen (ebna) are immunologically related, and may be identical. western blotting, using both anti-ebna and anti-alpha 2hs receptor serum identified a similar mr 48 000 component in labelled detergent-solubilized transformed cell supernatants. the mr 48 000 component recognized by anti-ebna serum had a pi between 7-8, sim ... | 1983 | 6311627 |
| aids update. | 1983 | 6311656 | |
| the igg subclass responses of human lymphocytes to b-cell activators. | the induction of igg synthesis by normal human lymphocytes in response to a range of b-cell activators has been studied by indirect cytoplasmic immunofluorescence using monoclonal antibodies to the four igg subclasses. considerable variation was found between cells from different donors tested with the same activator but the following trends were observed:- epstein-barr virus and branhamella catarrhalis induced a predominantly igg3 response, whereas the most prevalent cells in cultures stimulate ... | 1983 | 6311731 |
| comparison of the specific igm and igg antibody response in humans induced by antigen (tetanus toxoid) or a polyclonal activator (ebv) in vitro. | culture conditions were established for the reproducible induction of specific antibody responses in vitro using peripheral blood lymphocytes from tetanus toxoid (tt) immunized donors. maximum anti-tt antibody responses (250-350 ng/ml) were detected on day 9 of culture, with optimum quantities of antigen (0.1-1.0 micrograms/ml tt) or epstein-barr virus (ebv). the antigen-driven antibody response unlike the ebv-induced polyclonal response was both t-cell and monocyte-dependent. tt stimulated main ... | 1983 | 6311755 |
| proton-translocating atpase and lysosomal cystine transport. | a proton-translocating atpase was identified in highly purified lysosomes from epstein-barr virus-transformed human lymphoblasts. activity of this atpase caused acidification of highly purified, fluorescein isothiocyanate dextran-loaded lysosomes and correlated with the atp-dependent efflux of lysosomal cystine. the lysosomal atpase was distinct from mitochondrial f1-atpase in its responses to a variety of inhibitors. although atp-dependent lysosomal cystine efflux is not demonstrable in culture ... | 1983 | 6311822 |
| cell membrane potential changes follow epstein-barr virus binding. | upon binding to receptor-bearing target cells, viruses cause cell membrane potential changes. epstein-barr virus causes a biphasic membrane potential change in receptor-bearing b lymphocytes but not receptor-negative t lymphocytes, as measured by flow cytometry or cyanine dye uptake. membrane potential changes from ebv binding to receptor-bearing cells resemble electrical responses of other cells following ligand binding to transmembrane receptors. | 1983 | 6311847 |
| epstein-barr virus induces normal b cell responses but defective suppressor t cell responses in patients with systemic lupus erythematosus. | epstein-barr virus (ebv) is a polyclonal b cell activator, independent of helper t cells, which induces the generation of suppressor t cells in vivo and in vitro. given the complexity of the immunologic abnormalities in patients with systemic lupus erythematosus (sle), we used ebv as a tool to examine the following questions: a). are sle b cells primarily defective? and b). does ebv stimulate the generation of suppressor activity from sle t cells? it was found that b cells from sle patients infe ... | 1983 | 6311898 |
| monoclonal anti-parasite and anti-rbc antibodies produced by stable ebv-transformed b cell lines from malaria patients. | to produce human monoclonal antibodies associated with infectious disease, peripheral blood lymphocytes (pbl) from patients with plasmodium falciparum malaria were transformed with eb-virus in vitro. to enrich for malaria-specific b cells, pbl were incubated for 3 days with unsoluble p. falciparum antigen before ebv-transformation. furthermore, cyclosporin a was added during and after transformation to eliminate t cell suppression of b cell growth. microcultures were screened for antibodies agai ... | 1983 | 6311902 |
| enzyme-linked immunosorbent assay for the detection of epstein-barr virus-induced antigens and antibodies. | two epstein-barr virus (ebv)-specific elisa tests were developed. one, based on the use of crude extracts from virus producer cells highly induced in the presence of ara c (providing ea + vca- cells) or in the absence of the drug (providing ea + vca + cells) is suitable for the detection of antibodies directed against antigen complexes associated with the lytic virus cycle; i.e., ea, vca and presumably also ma. the second, performed with purified ebna, can be used for the detection of antibodies ... | 1983 | 6311908 |
| enzyme-linked immunosorbent assay for susceptibility to varicella. | nonspecific reactions to the membrane antigen of varicella-zoster virus were found by fluorescent antibody assay in the sera of three children susceptible to the virus; one of these children subsequently developed varicella. none of the three sera, however, contained antibody to varicella-zoster virus that was detectable by enzyme-linked immunosorbent assay (elisa). moreover, the results of the elisa clearly distinguished immune from susceptible individuals. the negative range was established wi ... | 1983 | 6311911 |
| chronic active epstein-barr virus infections in two immunodeficient patients. | 1983 | 6312007 | |
| sexually transmitted viral infections in homosexual men. | 1983 | 6312215 | |
| african type burkitt's lymphoma in a 69-year-old french caucasian woman. | 1983 | 6312410 | |
| acute cerebellar ataxia and infectious mononucleosis. | a 28-year-old man, who presented with acute cerebellar ataxia, was found to have haematological features of infectious mononucleosis. there was serological evidence of recent infection with epstein-barr virus. it is speculated that cerebellar dysfunction results from virus-induced inflammatory changes within the central nervous system. | 1983 | 6312442 |
| antibodies to epstein-barr virus associated antigens in pima indians with and without rheumatoid arthritis. | we investigated anti-rheumatoid arthritis-associated nuclear antigens (rana) and other anti-epstein-barr virus (ebv) antibodies in a uniquely controlled study in female pima indians of arizona, an ra prone population. four groups of age and sex-matched individuals were formulated: (1) individuals positive for rheumatoid factor (rf) who had clinical evidence of rheumatoid arthritis (ra); (2) individuals seropositive for rf, but without arthritis; (3) individuals seronegative for rf, but with vari ... | 1983 | 6312543 |
| acute transverse myelopathy in epstein-barr virus infection. case reports. | two patients with acute transverse myelopathy in association with epstein-barr virus infection are described. evidence of epstein-barr virus infection should be sought in any young patient with an acute neurological illness, even in the absence of the usual features of infectious mononucleosis. the cases described confirm previous reports that the prognosis in patients with neurological complications of this infection is generally good. | 1983 | 6312618 |
| [epstein-barr virus and its role in various rheumatic diseases]. | 1983 | 6312625 | |
| [nasopharyngeal carcinoma]. | 1983 | 6312656 | |
| infectious mononucleosis and syphilis testing: a similar history and future. | 1983 | 6312796 | |
| phosphorylation of acyclovir in vitro in activated burkitt somatic cell hybrids. | acyclovir [9-(2-hydroxyethoxymethyl)guanine] (acv), a potent antiviral compound, was phosphorylated to the same extent by extracts from untreated and iododeoxyuridine-treated epstein-barr virus-containing latent d98/hr-1 somatic hybrid cells. atp was the preferred phosphate donor over other nucleoside triphosphates. the cytosol extract from d98/hr-1 cells effected optimum phosphorylation of thymidine at ph 8.0, whereas acv was phosphorylated equally well over a wide ph range. electrophoretic ana ... | 1983 | 6312870 |
| effects of dna synthesis inhibitors on early antigen expression following primary infection or superinfection by epstein-barr virus. | seven lymphoid cell lines previously characterized with respect to their resident epstein-barr virus (ebv) genome content were infected or superinfected with concentrated ebv from supernatant of the p3hr-1 cell line. immunofluorescence assays were conducted on smears 48 hours after infection, using human sera containing antibodies to ebv early antigen (ea). two ebv nuclear antigen (ebna) negative cell lines containing no detectable resident ebv dna and five ebna positive cell lines containing eb ... | 1983 | 6312934 |
| acyclovir and epstein-barr virus infection. | acyclovir (acv) has an ed50 of 0.3 microm against ebv replication in vitro. based on these and other data we carried out a pilot, double-blind, placebo-controlled trial of acv for treatment of infectious mononucleosis. only patients with relatively severe illness requiring hospital management were enrolled. ten subjects with proven infectious mononucleosis received placebo and 10 acv. the drug was administered intravenously at 8-hourly intervals in a total daily dosage of 1500 mg/m2 for 5 days. ... | 1983 | 6313591 |
| use of acyclovir for prophylaxis of herpes infections in severely immunocompromised patients. | two consecutive studies are reported. in study 1, 59 patients with acute leukaemia undergoing remission induction therapy or bone marrow transplantation were given either acyclovir (5 mg/kg) or placebo by intravenous infusion twice daily during the period of neutropenia. all patients were seropositive (greater than or equal to 1:8) for herpes simplex virus. acyclovir provided total protection against herpes simplex virus infection in bone marrow transplant patients with 0/10 versus 5/10 infectio ... | 1983 | 6313594 |
| the spectrum of antiviral activities of acyclovir in vitro and in vivo. | in vitro sensitivity data suggest that acyclovir should be effective against clinical manifestations of herpes simplex virus types 1 and 2, varicella-zoster virus and possibly epstein-barr virus. the clinical potential against herpes simplex virus types 1 and 2 is further supported by results in animal models. human cytomegalovirus and the veterinary herpes viruses, with the possible exception of equine herpes virus type 1, may be insufficiently sensitive to be amenable to treatment. | 1983 | 6313598 |
| rheumatoid arthritis xii: epidemiology and genetics. | 1983 | 6306359 | |
| [the epstein-barr virus infects immunologic cells]. | 1983 | 6306366 | |
| clustered repeat sequences in the genome of epstein barr virus. | the genome of epstein-barr virus is composed of unique dna interspersed with repetitive sequences. this organization suggests that epstein-barr virus provides a useful model for studying the function(s) of repetitive sequences in eukaryotic chromosomes. the primary structure of two of the repeat sequences, the 3072 bp large internal repeat, or bamhi-w repeat, and a smaller 125 bp, g, c-rich noti repeat, are presented here. their structures and possible functions are discussed. | 1983 | 6306567 |
| gianotti-crosti syndrome in an infant with epstein-barr virus infection. | 1983 | 6306608 | |
| 9-([2-hydroxy-1-(hydroxymethyl)ethoxy]methyl)guanine: a selective inhibitor of herpes group virus replication. | 9-([2-hydroxy-1-(hydroxymethyl)ethoxy]methyl)guanine (2'-nor-2'-deoxyguanosine; 2'ndg) selectively inhibits the replication of herpes group viruses. in cell culture studies 2'ndg was at least 10-fold more potent than acyclovir (acv) in inhibition of human cytomegalovirus replication and epstein-barr virus-induced lymphocyte transformation and was about as effective as acv in inhibition of herpes simplex viruses 1 and 2 and varicella zoster virus. orally administered 2'ndg was 6- to 50-fold more ... | 1983 | 6306664 |
| epstein-barr virus: inhibition of replication by three new drugs. | epstein-barr virus (ebv) is the cause of infectious mononucleosis and is associated with three human malignancies. acyclovir [9-(2-hydroxyethoxymethyl)guanine], the first clinically useful drug effective against replication of ebv, is without effect against latent or persistent ebv infection. three nucleoside analogs, e-5-(2-bromovinyl)-2'-deoxyuridine, 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine, and 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-methyluracil are potent inhibito ... | 1983 | 6306771 |
| acyclovir. | acyclovir, the new virucidal drug recently approved by the food and drug administration for the treatment of herpes simplex virus (hsv), is the first available effective drug to treat such infections. acyclovir boasts activity against four of the five major herpes-group viruses, including herpes simplex (types 1 and 2), varicella-zoster, and epstein-barr. the drug, phosphorylated preferentially in viral cells, exclusively attacks infected cells possessing virus-specific dna polymerase, giving ac ... | 1983 | 6306847 |
| [acute cerebellar ataxia associated with infectious mononucleosis--a case report and review of the literature]. | a 20-year-old man, a college student, was admitted to kochi municipal central hospital with a month's history of slurring of speech and unsteadiness of gait. he had developed fever, sore throat and cervical lymphadenopathy. on admission, the throat was mildly injected, and enlarged lymph nodes were palpable in the lateral cervical regions. his speech was slightly slurred. bilateral dysmetria, dyssynergia and intention tremor were noted in both extremities. the gait was grossly ataxic. plantar re ... | 1983 | 6307327 |
| the relationship between the epstein-barr virus and rheumatoid arthritis. | 1983 | 6307454 | |
| acyclovir prophylaxis against herpes virus infections in severely immunocompromised patients: randomised double blind trial. | twenty patients undergoing allogeneic bone marrow transplantation and 39 patients receiving remission induction chemotherapy for acute leukaemia were entered into a double blind, placebo controlled stratified trial of acyclovir prophylaxis against herpes group virus infections. within the transplant group intravenous acyclovir 5 mg/kg twice daily given throughout the period of granulocytopenia completely prevented oropharyngeal herpes simplex virus infection compared with a 50% incidence in the ... | 1983 | 6307464 |
| limiting dilution analysis of epstein-barr virus-induced immunoglobulin production. | the major goal of this work is to establish a culture system for the growth of human b lymphocytes at the single-cell level so that the immunoglobulin secreted by the clonal progeny of that cell can be analyzed. a method which involves culturing small numbers (1-1000) of lymphocytes, which have been infected with epstein-barr virus (ebv) prior to plating, in round-bottom microtiter plates is described. a feeder layer of irradiated (2500 r) umbilical cord blood lymphocytes to which phytohemagglut ... | 1983 | 6307533 |
| effects of cyclosporin a on immunoglobulin production by eb virus stimulated lymphocytes. | cyclosporin a (cy a) enhances immunoglobulin (ig) production by eb virus stimulated peripheral blood lymphocytes from normal subjects with prior immunity to eb virus. in 11 normal adults, this enhancement of ig production correlated with t cell-mediated cytotoxic regression. cultures from normal healthy adults without prior immunity showed a diminution of ig production when cy a was added. however, cy a had no effect on ig production by cord blood lymphocytes in the first week of culture but the ... | 1983 | 6307560 |
| a human b-cell line (bes-1) forming rosettes with sheep erythrocytes, producing forssman antibody, and phagocytizing sheep erythrocytes. | a lymphoid cell line, designated bes-1, belonging to "lymphoblastoid cell lines with b-cell properties (b-lcl)" and derived from some normal b lymphocytes, was established in an attempt at primary culture of peripheral blood from a patient with acute myelogenous leukemia (aml). bes-1 cells unexpectedly formed rosettes with sheep erythrocytes (srbc), which are usually a t-cell marker, and phagocytized srbc. subsequent study indicated that bes-1 cells produce antibody specific to forssman (f) anti ... | 1983 | 6307565 |
| frequency of anti-dna antibody producing cells from normals and patients with systemic lupus erythematosus. | the frequency of anti-dna antibody producing cells from normals and patients with systemic lupus erythematosus (sle) was determined. peripheral blood lymphocytes (pbl) from normals and patients with sle were cultured for 8 and 15 days with and without transformation by epstein-barr virus (ebv). culture supernatants were examined for the presence of anti-dna antibody using an enzyme-linked immunosorbent assay. we found that pbl from patients with sle spontaneously produce anti-dna antibodies wher ... | 1983 | 6307568 |
| studies on the relationship between hla dr4 and in vitro igm rheumatoid factor production. | the relationship between the presence of the dr4 antigen and other hla antigens and in vitro production of igm-rheumatoid factor by lymphocytes from a group of healthy young subjects was examined. pokeweed and epstein--barr virus-stimulated lymphocyte cultures were examined for the production of rheumatoid factor and immunoglobulins. no significant correlation was found between the presence of the dr4 antigen and in vitro production of igm rheumatoid factor. the presence of the b18 antigen seeme ... | 1983 | 6307569 |
| elevated antibody titers to epstein-barr virus and low natural killer cell activity in patients with chediak-higashi syndrome. | four venezuelan patients with the autosomal recessive chediak-higashi syndrome (chs) were studied. the results confirm the severe reduction in natural killer (nk) cell activity, as previously described and showed also a decline in the activity of cells involved in antibody-dependent cellular cytotoxicity (adcc). no defect was found in the production of immunoglobulins and of specific antibodies to measles, varicella, herpes simplex, and cytomegalo viruses. two of the patients had extremely high ... | 1983 | 6307571 |
| nucleic acid hybridization for the detection of viral genomes. | 1983 | 6307595 | |
| studies on nasopharyngeal carcinoma in the sudan--i. epidemiology and aetiology. | three hundred and seventy-four cases of nasopharyngeal carcinoma (npc) recorded in the sudan cancer registry (scr) and 512 cases seen at the radiation and isotope centre, khartoum (rick) were analysed. npc formed 5.8% of all cancer cases in the scr and 7.2% at the rick; this is the highest frequency so far reported outside the chinese. the male/female ratio was 3:1; npc was the commonest tumour in males at the rick (12.1%) and second commonest in the scr (9.2%). it tended to occur in younger pat ... | 1983 | 6307705 |
| [new aspects in kaposi's sarcoma]. | 1983 | 6307839 | |
| association of some supraglottic laryngeal carcinomas with eb virus. | thin sections of biopsies from five patients with supraglottic laryngeal carcinoma who possessed antibody to early antigen of eb virus were examined for the presence of eb virus nuclear antigen (ebna) and eb virus dna by cytological hybridization in situ. both ebna and eb virus dna were found in the tumour cells of three of the patients. the present findings suggest an association between eb virus and supraglottic carcinoma of the larynx. | 1983 | 6307894 |
| antigenicity of hla-d locus antigens in human cell-mediated lympholysis. | 1983 | 6308312 | |
| infectious mononucleosis in patients aged 40 to 72 years: report of 27 cases, including 3 without heterophil-antibody responses. | epstein-barr-virus (ebv)-induced infectious mononucleosis usually occurs in young adults between the ages of 15 and 30. when it occurs in older individuals, it frequently presents diagnostic problems. this report describes data from 27 such patients aged 40 to 72, all of whom had definitive evidence of a current ebv primary infection. protracted fever, jaundice, pleural effusion, anemia, or the guillain-barré syndrome were dominant clinical findings among these patients. fourteen patients were h ... | 1983 | 6308383 |
| application of epstein-barr virus serology to the diagnosis and staging of north american patients with nasopharyngeal carcinoma. | from 1978 to 1981, 151 patients with nasopharyngeal carcinoma (npc) were enrolled in a prospective, collaborative study of north american patients, most of them white. thirty-seven had world health organization (who) type 1 tumors, and 114 had who types 2 and 3 tumors. the anti-epstein-barr virus (ebv) profile of elevated antibody titers directed against viral capsid antigen and early antigen was seen in 85% of the patients with who types 2 and 3 tumors but in only 16% of the patients with who t ... | 1983 | 6308537 |
| viral infection in the renal transplant patient. | with the advances that have occurred over the last two decades in the prevention and treatment of bacterial and fungal infection, viral infection has been recognised as an important problem in renal transplant patients. four groups of viruses--the herpesviruses, hepatitis viruses, papovaviruses, and adenoviruses - appear to have a particular impact on this patient population, especially the first two of these. the effects of these viruses can be categorised as follows: the production of infectio ... | 1983 | 6308602 |
| analyses of bromodeoxyuridine-associated sister chromatid exchanges (sces) in bloom syndrome based on cell fusion: single and twin sces in endoreduplication. | when bloom syndrome (bs) cells labeled with bromodeoxyuridine (brdurd) for one round of dna replication were fused with nonlabeled normal cells, the hybrid cells had a normal level of sister chromatid exchange (sce) at the first mitosis after fusion. however, when normal cells treated with mitomycin c (mc) were fused with nontreated normal cells, the mc-induced sce was not affected by fusion with normal cells. single and twin sces were analyzed in the colcemid-induced endoreduplicated normal and ... | 1983 | 6308619 |
| efficient generation in vitro, from human peripheral blood cells, of monoclonal epstein-barr virus transformants producing specific antibody to a variety of antigens without prior deliberate immunization. | this paper describes a simple protocol for the efficient generation of large numbers of human monoclonal antibody-producing cells. this system is based on initial limiting-dilution culture after epstein-barr virus exposure of highly enriched precursors selected from peripheral blood mononuclear cells. precursors can be enriched by using rosetting or panning approaches. antibodies to erythrocytes, a mouse mammary carcinoma, dna, and sperm antigens, produced without any deliberate immunization, ar ... | 1983 | 6308627 |
| two small rnas encoded by epstein-barr virus can functionally substitute for the virus-associated rnas in the lytic growth of adenovirus 5. | adenovirus (ad) serotypes 2 and 5 synthesize large amounts of two low molecular weight rnas designated virus-associated (va) 1 and 2. recently, genetic and biochemical approaches have been used to show that ad2 va1 rna is required for efficient translation of viral mrnas produced at late times after infection. primate cells harboring the epstein-barr virus genome (ebv) synthesize large amounts of two low molecular weight rnas: eber1 and eber2. striking similarities of gene organization have been ... | 1983 | 6308649 |
| rearrangement and amplification of c-abl sequences in the human chronic myelogenous leukemia cell line k-562. | structural rearrangements of specific cellular sequences (c-onc genes) homologous to acute transforming retrovirus oncogenes (v-onc genes) have been recently described in various malignancies. here we show that human cellular sequences (c-abl) homologous to the transforming sequences of the mouse abelson leukemia virus (v-abl) are amplified some 4- to 8-fold in k-562, a philadelphia chromosome-positive cell line derived from a patient with chronic myelogenous leukemia in blast crisis. restrictio ... | 1983 | 6308652 |
| differential expression of the normal and of the translocated human c-myc oncogenes in b cells. | we have investigated whether the translocated and the untranslocated human c-myc oncogenes of burkitt lymphoma cells are equally or differentially expressed in host mouse b cells. the human c-myc mrna levels in somatic cell hybrids between mouse plasmacytoma cells and burkitt lymphoma cells with either the t(8;14) or the t(2;8) chromosome translocation were determined by using the nuclease s1 protection procedure. although both the human parental lines and the hybrid cells carrying the transloca ... | 1983 | 6308654 |
| the igg subclass distribution of thyroglobulin antibody synthesized in culture. | thyroglobulin autoantibodies synthesized by hashimoto lymphocytes in culture and present in serum have been analysed in terms of their igg subclass distribution. the autoantibodies produced in vitro were frequently igg4 or igg1, whether pokeweed mitogen or epstein-barr virus was used to stimulate the cultures, and the subclass distribution of these thyroglobulin antibodies was similar to that observed in the patients' serum. it appears therefore that the antibodies synthesized in vitro in respon ... | 1983 | 6308751 |
| post-perfusion syndrome due to epstein-barr virus. report of two cases and review of the literature. | the post-perfusion syndrome is rarely due to epstein-barr virus (ebv), because most adult patients already carry protective neutralizing antibodies in their sera. data are presented from two patients in whom heterophil-antibody-positive infectious mononucleosis developed 35 and 48 days after open-heart surgery. the diagnoses of their predominantly febrile illnesses were delayed because of late appearance of atypical lymphocytes and heterophil antibodies. ebv-specific antibody responses were also ... | 1983 | 6308858 |
| morphological evidence of viruses in nasopharyngeal carcinoma? | human tumor tissue from patients with nasopharyngeal carcinoma was investigated electron microscopically before and after transplantation to nude mice. although we were unable to find distinct herpes type-c particles in human tissue, these particles could be seen following tissue transplantation to nude mice in two cases. the findings are discussed as to whether those particles are either endogenous in nude mice or expressed by the tumor tissue as a consequence of transplantation. | 1983 | 6308945 |
| national case-control study of kaposi's sarcoma and pneumocystis carinii pneumonia in homosexual men: part 2. laboratory results. | the centers for disease control conducted a case-control study to investigate an outbreak of kaposi's sarcoma and pneumocystis carinii pneumonia in homosexual men. the occurrence of these diseases was found to be associated with certain aspects of lifestyle, including a greater number of male sex partners per year, exposure to feces during sex, history of syphilis and non-b hepatitis, treatment for enteric parasites, and use of various illicit substances. laboratory studies reflected both this l ... | 1983 | 6309049 |
| [epstein-barr virus infection in children: study of infectious mononucleosis without heterophil antibodies]. | 1983 | 6309061 | |
| epstein-barr virus-specific serodiagnostic tests in carcinomas of the head and neck. | sera from 256 patients with cancers of the head and neck were examined for their profiles of igg and iga antibodies to epstein-barr virus (ebv)-specific, viral capsid antigen (vca), the diffuse (d) and the restricted (r) components of the early antigen (ea) complex, and the ebv-associated nuclear antigen (ebna), in order to assess the value of these procedures in the routine diagnosis of poorly or undifferentiated nasopharyngeal carcinoma (npc). in 13 npc patients, the carcinoma had invaded cerv ... | 1983 | 6309355 |
| karyotypic findings in a case of prolymphocytic leukemia with a history of radiation exposure. | cytogenetic examination, utilizing b- and t-cell mitogens, of the peripheral blood lymphocytes of a patient with the prolymphocytic variant of chronic lymphocytic leukemia (cll) and a history of radiation exposure revealed two abnormal clones. one clone with 48 chromosomes (+t(6;12),6q-, +12,14q+) may be derived from cll cells, whereas the clone with 46 chromosomes and a ring #11 possibly originated from normal t and/or b cells affected by past irradiation. | 1983 | 6309359 |
| epstein-barr virus-activating principle in the ether extracts of soils collected from under plants which contain active diterpene esters. | soil samples were collected from the ground under the plants of euphorbiaceae and thymelaeaceae known to possess epstein-barr virus-activating diterpene esters. in a test system, the ether extracts of such soil samples at a concentration of 20 micrograms/ml induced epstein-barr virus early antigen in approximately 5-25% of the non-producer raji cells. these findings suggest a possible interaction between plant-derived diterpene esters and the human system, and provide a new aspect in considering ... | 1983 | 6309360 |
| chinese and african euphorbiaceae plant extracts: markedly enhancing effect on epstein-barr virus-induced transformation. | chinese and african euphorbiaceae plant extracts were shown to have a markedly enhancing effect on epstein-barr virus (ebv)-induced transformation of human lymphocytes. when 5 x 10(5) cord blood lymphocytes were seeded into the semisolid agar medium immediately after ebv exposure, 3-10 times more colonies developed in the presence of the plant extracts at their optimal doses. when a smaller number of 5 x 10(4) cells were seeded, transformed colonies were also observed in the presence of the plan ... | 1983 | 6309363 |
| self-stimulating growth factor production by b-cell lines derived from burkitt's lymphomas and other lines transformed in vitro by epstein-barr virus. | kinetics of cell growth of human b-lymphocyte lines derived from burkitt's lymphomas and lines derived from the epstein-barr virus transformation of normal adult lymphocytes were studied. cells seeded at high densities (5 x 10(4)/ml) grew in an exponential manner; those seeded at low densities (5 x 10(3) to 1 x 10(4) began to grow exponentially only after a lag period of 3 to 4 days. transfer of stationary low-density cell populations into filtered media from growing high-density cultures (condi ... | 1983 | 6309371 |
| enzyme-linked immunosorbent assay for antibodies to epstein-barr virus-associated early antigens and the viral capsid antigen. | the determination of serum antibody titers to epstein-barr virus (ebv)-associated antigens is of diagnostic and prognostic importance in the case of patients with ebv-associated tumors, ie, burkitt's lymphoma and nasopharyngeal carcinoma, and in infectious mononucleosis. enzyme-linked immunosorbent assays (elisa) were developed for the detection of antibodies to ebv-associated early antigens (ea) and the viral-capsid antigen. the antibody titers obtained by the elisa method correlate well with t ... | 1983 | 6309387 |
| clinical consequences of epstein-barr virus infection and possible control by an anti-viral vaccine. | 1983 | 6309441 | |
| characterization of the suppressor activity in lymphocytes from patients with common variable hypogammaglobulinemia: evidence for an associated primary b-cell defect. | the pathogenetic mechanisms responsible for the impaired immunoglobulin production in common variable hypogammaglobulinemia (cvh) are diverse with abnormalities in both b cells and immunoregulatory t cells. production of igg, igm, and igm-rheumatoid factor (igm-rf) was measured in pokeweed mitogen (pwm) or epstein-barr virus (ebv)-stimulated cultures using various combinations of cvh, cord blood mononuclear cells (cbmc), and normal adult control b and t cells. the following results were obtained ... | 1983 | 6309448 |
| immunological control of epstein-barr virus-transformed lymphocytes in multiple sclerosis. | t-cell control of epstein-barr virus (ebv)-infected autologous lymphocytes was examined in 25 multiple sclerosis (ms) patients and in 25 healthy individuals, all seropositive for ebv. by assessing regression of cell transformation, 17 patients and all healthy subjects were scored as "regressors." eight ms patients proved to be "nonregressors," indicating loss of cytotoxic t-cell control. in this group there was apparently a concurrent fall in the mean titer of antibody to epstein-barr nuclear an ... | 1983 | 6309449 |
| epstein-barr virus specific t-cell response in nasopharyngeal carcinoma patients. | there is a substantial body of evidence suggesting an association between epstein-barr virus (ebv) and undifferentiated nasopharyngeal carcinoma (npc). the present study has compared a group of npc patients (newly diagnosed and long-term survivors) and controls for ebv-specific t-cell immunity using the regression of transformation assay. newly diagnosed patients (17 tested) when compared with either long-term survivors (20 tested) or controls (30 tested) showed a significant impairment in virus ... | 1983 | 6309678 |
| purification of a protein (60k/58k) associated with the epstein-barr virus-induced early antigen complex in raji cells. | a double antibody sandwich elisa has been established for the detection and quantitation of ebv-associated early antigens (ea) in iudr-induced raji cells. the ea complex extracted from raji cells could be separated by ion exchange chromatography and isoelectric focusing into several components. one ea-associated subspecificity has been purified by deae-, cm-, and blue-sepharose chromatography followed by isoelectric focusing. the isolated protein has an apparent molecular weight of 240,000 +/- 2 ... | 1983 | 6309679 |
| epstein-barr virus (ebv), lymphocytes and transformation. | 1983 | 6309856 | |
| a culture method giving substantial yields of normal nasopharyngeal epithelial cells for work with epstein-barr virus. | a culture method, utilising a feeder layer of lethally irradiated 3t3 fibroblasts and medium supplemented with hydrocortisone, cholera toxin, and epidermal growth factor, has been elaborated for the in vitro growth of normal human nasopharyngeal epithelial cells. this method allowed the cells to be grown in vitro for periods of up to 146 days, very considerably longer than in previously reported studies, and ensured that the cultures remained largely free from contaminating human fibroblasts. it ... | 1983 | 6309877 |
| oncological consequences of impaired immune surveillance against ubiquitous viruses. | the immune system is constantly challenged by ubiquitous viruses. multiple immune defenses have evolved to meet these challenges, and thus immunocompetent individuals successfully respond to infection without sequela. x-linked lymphoproliferative syndrome patients, renal allograft recipients, and acquired immunodeficiency syndrome patients share impaired immune surveillance as a common feature. such individuals are variously susceptible to numerous untoward complications following infection with ... | 1983 | 6309892 |
| acquired hypogammaglobulinemia following infectious mononucleosis. | the case of a 17-year-old male who developed hypogammaglobulinemia following infectious mononucleosis is presented. the family history revealed that a male sibling had died some years earlier with encephalitis also following infectious mononucleosis. laboratory investigations revealed adequate numbers of t and b cells and normal proportions of helper and suppressor t lymphocytes but poor in vitro responses to mitogens and an absence of hypersensitivity to skin test antigens. serial serological e ... | 1983 | 6309893 |
| epstein-barr virus-induced immunoglobulin synthesis by b cells from individuals with late-onset panhypogammaglobulinemia. | epstein-barr virus (ebv) transformation was used to examine the differentiation potential of circulating b cells from eight individuals with late-onset panhypogammaglobulinemia. cytoplasmic and secreted immunoglobulins were evaluated by immunofluorescence and radioimmunoassay. ebv-infected cultures of b cells from patients and healthy controls generated similar numbers of igm-secreting plasma cells, but relatively few igg and iga plasma cells were induced in cultures of patients' b cells. as fur ... | 1983 | 6309895 |
| immunoregulatory human t lymphocytes triggered as a consequence of viral infection: clonal analysis of helper, suppressor inducer and suppressor effector cell populations. | the regulatory functions of a series of human t cell clones specific for an autologous epstein-barr virus transformed b lymphoblastoid cell line were examined. two t4+ t cell clones, termed at4ii and at4iv, and one t8+ clone, at8iii, were maintained in culture for greater than or equal to 9 months and were characterized in detail. both t4+ clones provided helper function for autologous b cell immunoglobulin production when added to unstimulated peripheral blood mononuclear cells. in addition, th ... | 1983 | 6309959 |
| a study of the functional capabilities of human neonatal lymphocytes for in vitro specific antibody production. | the ability of neonatal b and t cells to participate in the in vitro production of anti-influenza virus antibody was studied. peripheral blood mononuclear cells from nearly all normal adults produce anti-influenza virus antibody when stimulated in vitro with type a influenza virus. cord blood mononuclear cells, however, consistently failed to do so. using epstein-barr virus activation or coculture with irradiated adult t cells in the presence of influenza virus to identify precursor b cells for ... | 1983 | 6309961 |
| epstein-barr virus early antigen induction in raji cells by chinese medicinal herbs. | ether extracts of 495 chinese medicinal herbs from 106 families were studied for epstein-barr virus (ebv) early antigen (ea) induction in the raji cell system. 15 herbs from 10 families were found to have inducing activity. water extracts of the same herbs also had inducing activity, but it was not as strong. the significance of these herbs in the activation of ebv in vivo and their relation to the development of nasopharyngeal carcinoma are discussed. no ea-inducing activity was found in 73 sam ... | 1983 | 6305871 |
| human cytomegalovirus specific iga antibodies detected by immunoperoxidase assay in serum of patients with cytomegalovirus infections. | cytomegalovirus (cmv)-specific iga antibodies were determined by an immunoperoxidase assay in sequential serum samples of 10 patients with cmv infection in order to evaluate the feasibility of the use of this technique for diagnosis. in parallel, igm and igg antibodies to cmv were studied by enzyme-linked immunosorbent assay (elisa) and by the immunoperoxidase assay, respectively. cmv iga antibodies were detected in all 10 cmv patients studied. specific igm was detected earlier than iga in only ... | 1983 | 6306032 |
| x-linked lymphoproliferative syndrome. natural history of the immunodeficiency. | the x-linked lymphoproliferative syndrome is characterized by immunodeficiency to epstein-barr virus (ebv) manifested by severe or fatal infectious mononucleosis and acquired immunodeficiency. we studied immune responses in six males of a well-characterized kindred with the x-linked lymphoproliferative syndrome. two males were studied before and during acute fatal ebv infection. both individuals demonstrated normal cellular and humoral immunity before ebv infection. during acute ebv infection, b ... | 1983 | 6306053 |
| t cell factor-mediated suppression of immunoglobulin secretion by a human b lymphoblastoid cell line. | the epstein barr virus-transformed b lymphoblastoid cell line jr-2, which spontaneously secretes igg detectable by a hemolytic plaque technique, is suppressed by the concanavalin a-induced t cell-derived nonspecific suppressor factor t-sf in a dose-dependent manner. this suppression is reversible after 24 hr and is not due to a cytotoxic mechanism. the t-sf does not affect proliferation of jr-2. suppression by the t cell factor is inhibited or blocked by 2-mercaptoethanol or l-rhamnose and is al ... | 1983 | 6306101 |
| analysis of the defects responsible for the impaired regulation of ebv-induced b cell proliferation by rheumatoid arthritis lymphocytes. ii. role of monocytes and the increased sensitivity of rheumatoid arthritis lymphocytes to prostaglandin e. | diminished regulation of ebv-induced b cell proliferation by t cells from patients with rheumatoid arthritis (ra) is paralleled by diminished production of gamma-interferon in response to autologous but not allogeneic stimulation. we have shown that the adherent cell subpopulation within the autologous ra stimulators plays a major role in the ra defect. in analyzing the mechanisms responsible for the adherent cell effect in ra, we examined the contribution of prostaglandin production. indomethac ... | 1983 | 6306107 |
| complement receptor expression by neoplastic and normal human cells. | complement receptor (cr) expression in cell lines derived from burkitt's lymphoma (bl), epstein barr virus-transformed cord blood lymphocytes (cb), and peripheral lymphocytes from patients with infectious mononucleosis (im) was examined. red cell intermediates bearing various densities of c4b, c3b, c3bi, or c3d were tested for rosette formation with the cell lines. in addition, a series of studies was performed under conditions that precluded the cleavage of cellbound c3b by factor i (c3bina). t ... | 1983 | 6306108 |
| consequences of infections by epstein-barr virus. | 1983 | 6306186 | |
| identification of polypeptide components of the epstein-barr virus early antigen complex with monoclonal antibodies. | three monoclonal antibodies were produced against the epstein-barr virus-induced early antigen complex. these antibodies were shown to be specific for the early antigen complex by the fact that they only reacted with cells supporting a permissive or abortive epstein-barr virus infection and their synthesis was not affected by inhibitors of viral dna synthesis. one monoclonal antibody, designated r3, was directed against a diffuse component of the early antigen complex since it reacted by immunof ... | 1983 | 6306272 |
| cytomegalovirus isolations from cell cultures derived from epstein-barr virus-associated nasopharyngeal carcinoma. | cytomegalovirus (cmv) was isolated in cell cultures derived from 2 of 11 nasopharyngeal carcinoma (npc) biopsy specimens from north african patients. all these cases were epstein-barr virus (ebv)-associated npc. morphologic cytopathic changes and viral replication not associated with ebv were observed after 2 months in culture. virus identification was achieved by immunofluorescence studies, and cell culture antigens were tested by the use of complement fixation and indirect hemagglutination. al ... | 1983 | 6306325 |