Publications

polymicrobial septicemia due to shigella flexneri and pseudomonas aeruginosa: first report.	an invasion of the bloodstream by members of the genus shigella is extremely rare. when it does occur, the etiologic agent is usually a single organism. a report of the first case of shigella bacteremia that was complicated by pseudomonas aeruginosa sepsis is reported.	1987	3123706
effects of cadmium on human platelet reactions.	human platelets incubated with cd2+ took up the cation slowly, and the uptake was speeded up by ionophore a23187. the capacity of human platelets to accumulate cd2+ was large, equivalent to 10 nmol cd2+ per mg protein. the effects of cd2+ on protein phosphorylation and serotonin release of human platelets were studied. washed platelets incubated with cd2+ showed a general increase in protein phosphorylation concurrent with a slow release of serotonin. in the presence of ionophore a23187, however ...	1987	3125819
pulmonary clearance of pseudomonas aeruginosa in neutropenic mice. effects of systemic immunization.	systemic immunization with killed pseudomonas improves pulmonary clearance of these bacteria in normal mice. to test the effects of active systemic immunization on early pulmonary clearance of pseudomonas in neutropenic mice, balb/c mice were intraperitoneally immunized with 10(8) pseudomonas or injected with saline twice weekly for 2 wk. ten days after the last immunization, immunized and control mice received tail vein injections with either nitrogen mustard or saline daily for 3 days. two day ...	1987	3109290
inner-city survey for tuberculosis: evaluation of diagnostic methods.	a total of 1,271 persons living in a socially and economically depressed, inner-city area of vancouver, british columbia, voluntarily attended a tuberculosis case-finding campaign. chest x-ray, on-the-spot specimen of sputum, and tuberculin skin test were offered at the time of the first attendance. all 3 diagnostic methods were found to be well accepted, with 93% of the participants having an x-ray, over 95% producing a sputum specimen, and almost 95% having a tuberculin test (a quarter of thes ...	1987	3109292
effects in rats of dietary protein inadequacy on lactose production, milk volume and components of the lactose synthetase complex (ec 2.4.1.22).	lactose synthetase (ls) is a complex of alpha-lactalbumin and n-acetylglucosamine galactosyl transferase (gt). ls is the rate-limiting enzyme in lactose biosynthesis. lactose is the main osmotic component of milk and may thereby affect milk volume. dietary protein inadequacy reduces milk volume and lactose content. the study investigates the role of ls activity in these phenomena. lactating rats were fed adequate and inadequate amounts of protein of high (milk protein) and low (cereal protein) q ...	1987	3109312
purification and characterization of dihydrofolate reductase from soybean seedlings.	dihydrofolate reductase (dhfr; ec 1.5.1.3) was purified to homogeneity from soybean seedlings by affinity chromatography on methotrexate-aminohexyl sepharose, gel filtration on ultrogel aca-54, and blue sepharose chromatography. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the enzyme gave a single protein band corresponding to a molecular weight of 22,000. the enzyme is not a 140,000 da heteropolymer as reported by others. amino acid sequence-specific antibodies to intact human d ...	1987	3109322
studies on the rate-determining factor in testosterone hydroxylation by rat liver microsomal cytochrome p-450: evidence against cytochrome p-450 isozyme:isozyme interactions.	the aim of the present study was to examine a recent proposal that inhibitory isozyme:isozyme interactions explain why membrane-bound isozymes of rat liver microsomal cytochrome p-450 exert only a fraction of the catalytic activity they express when purified and reconstituted with saturating amounts of nadph-cytochrome p-450 reductase and optimal amounts of dilauroylphosphatidylcholine. the different pathways of testosterone hydroxylation catalyzed by cytochromes p-450a (7 alpha-hydroxylation), ...	1987	3109324
coagulation defects of aflatoxin intoxicated rabbits.	twelve new zealand white rabbits were intoxicated with aflatoxin b1. most rabbits developed a coagulation defect near the time of death. immediately prior to death there were significant decreases in factors v, vii, and viii coagulant activities and fibrinogen concentration without a change in plasma fibrin(ogen) degradation product concentration, platelet number, and detectable plasma fibrin monomers. microscopic evidence of disseminated intravascular coagulation was present in one rabbit with ...	1987	3103313
characterization of self-transmissible plasmids determining lactose fermentation and multiple antibiotic resistance in clinical strains of klebsiella pneumoniae.	the lactose fermentation (lac+) and antibiotic resistance (r+) phenotypes were conjugally transferred from klebsiella pneumoniae strains (k166, k182, k186, k218, and k220) to salmonella typhi, s. typhimurium, shigella flexneri, and vibrio cholerae. the genes for lactose fermentation and antibiotic resistance were located on the plasmids. further analysis of plasmid dna from these isolates indicated the presence of multiple plasmids (mr ranged less than 2.7 to 70 x 10(6)). the lac+r+ plasmids p16 ...	1987	3107002
osteonectin content in human osteogenesis imperfecta bone shows a range similar to that of two bovine models of oi.	samples of human osteogenesis imperfecta (oi) bone were analyzed for osteonectin content by sds gel electrophoresis and immunodetection on western blots. the oi bone osteonectin content varied from normal to severely depressed. previously, we showed that two clinically identical but genetically unrelated bovine models of oi were differentiated biochemically by their bone osteonectin content: one oi model had normal bone osteonectin while the other was severely depressed in this parameter. the da ...	1987	3107778
in vivo studies on intracellular ph, focal flow, and vessel diameter in the cat cerebral cortex: effects of altered co2 and electrical stimulation.	the time course of changes in cortical tissue ph (phi) and blood flow during cortical seizures in halothane-anesthetized cats was examined. the clearance of the molecular form of umbelliferone (um) was used to estimate focal cortical blood flow (cbfu), whereas the ratio of the molecular to the ionic form of the molecule was used to concurrently calculate the local phi. resting phi and flow in normocarbic animals was 7.116 +/- 0.008 and 46 +/- 8 ml/100 g/min, respectively. respiratory induced alt ...	1987	3108271
effects of growth hormone-releasing hormone and corticotropin-releasing hormone on the release of thyrotropin-releasing hormone from the rat hypothalamus in vitro.	effects of growth hormone-releasing hormone (grh) and corticotropin-releasing hormone (crh) on the release of immunoreactive thyrotropin-releasing hormone (ir-trh) from the rat hypothalamus in vitro were studied. the rat hypothalamus was incubated in medium 199 with 1.0 mg/ml of bacitracin (ph 7.4) for 20 min. the amount of ir-trh release into the medium was measured by radioimmunoassay. the ir-trh release from the rat hypothalamus was inhibited significantly in a dose-related manner with the ad ...	1987	3131150
on the mechanism of antithrombotic action of flavonoids.	flavonols (quercetin and rutin) and flavanes (cyanidol and meciadonol) were studied for their effect on non-enzymatic lipid peroxidation, lipoxygenase and cyclo-oxygenase activities, binding to albumin and platelet membranes. these biochemical properties of four flavonoids were compared with respect to their antithrombotic action in vivo and their efficacy at influencing the platelet-endothelium interaction in vitro. all four flavonoids inhibited the ascorbate-stimulated formation of malondialde ...	1987	3101704
hypersensitivity to shigella outer membrane proteins in mice.	mice sensitized with living sh. flexneri 3a or with outer membrane proteins (omp) derived from these bacteria showed arthus and delayed type hypersensitivity (dth) when injected in the footpad with omp. the delayed reactivity to omp was transferable to normal mice by spleen cells obtained from donor animals which were previously sensitized with living bacteria.	1987	2977277
the pattern of atrial natriuretic peptide release during ventricular tachycardia in man.	1. plasma levels of atrial natriuretic peptide (anp) are high in many patients with tachycardia, but patterns of release with onset and termination of tachycardia and relationships to haemodynamic recordings are not clear. blood for anp measurements was therefore drawn from the coronary sinus, femoral artery and femoral vein, and simultaneous haemodynamic recordings were made in five patients before, during and after induction of stable ventricular tachycardia for 30 min. 2. tachycardia induced ...	1987	2963719
high affinity aldosterone binding sites (type i receptors) in rat heart.	1. the use of the receptor stabilizing agent sodium molybdate, and of ru26988 to exclude [3h]-aldosterone binding from type ii glucocorticoid receptors, has enabled the characterization of high affinity type i aldosterone binding sites in rat atrial and ventricular cytosols. 2. in adult male and female rats the affinity of binding (kd 4 degrees c) is approximately 1-2 nmol/l for both atria and ventricles; specificity of binding is similar to that for type i sites in classical aldosterone target ...	1987	2966024
why patient education. ii. the educational experience--planning, implementing and evaluating teaching for patients with diabetes mellitus.		1987	259961
migrant health question.		1987	259979
do surgeons know about aorn standards?		1987	260641
enzymology--part 1.		1987	260526
effect of the standard on size of the horizontal-vertical illusion.	70 subjects participated in a study designed to replicate the 1968 claim of gardner and long that the horizontal-vertical illusion is larger with the vertical than with the horizontal as standard. the effect did not appear in exp. 1 when subjects made judgments under both the reproduction and graded-series methods but did in a pilot study and in exp. 2, although more strongly with the method of reproduction.	1987	2235250
[nursing in uproar?].		1987	260379
[norwegian health workers in lebanon].		1987	260367
[determination and application of the so-called biological age].		1987	211834
inactivation of bacteria in sewage sludge by gamma radiation.	the survival of certain bacterial cultures suspended in sewage sludge and exposed to gamma-radiation was studied. the inactivation patterns of most of the organisms were significantly different when irradiation was performed using sewage samples collected in the summer and monsoon seasons. the summer sample collected from the anaerobic digestor afforded significant protection to both gram negative and gram positive organisms. this was evident by the increase in dose required to bring about a 6 l ...	1987	15092791
[combined resections in the surgery of lung cancer].	operations were performed on 3,313 patients for lung carcinoma in the department of surgery of the lungs and mediastinum of the national research center of surgery, ams ussr, from 1963 till 1.01.90. among these patients 263 (7.9%) were treated by combined operations (resection of the lung together with part of the chest wall, trachea and its bifurcation, pericardium, diaphragm, superior vena cava, pulmonary artery, aorta, and atrium, and also with removal of metastatic lymph nodes of the contral ...	1988	1434353
linear dichroic spectra and fluorescence polarization of biliverdin.		1988	1141595
nursing preferences for clientele.		1988	259418
conduct and utilization of clinical nursing research.		1988	259389
the radiological features of alcoholic ulcero-osteolytic neuropathy in blacks.	the radiological features in the bones and the angiographic changes in the vessels of the lower limbs of 15 chronic alcoholic men are discussed. the distal arteries demonstrate the features of neovascularization. infective and resorptive changes in the bones of the forefeet are described. these features are related to chronic infection or to the toxic effects of the alcohol.	1988	211654
[report on health facilities. 12. midwifery practice at the obstetrical department of kanto teishin hospital: emphasis on health education at a hospital associated with an industry].		1988	259673
guidance on symptoms of illness in babies: a further service from the fsid.		1988	259593
spontaneous rupture of the urinary bladder.		1988	266610
parallel studies of hla antigens in patients with rheumatic heart disease and scleritis: comparisons with three control populations.	parallel studies were carried out on hla antigens in patients with rheumatic heart disease and scleritis. comparison with one control population showed a significant excess of bw15 in both disease samples, while a comparison with two other control populations, showed the excess not to be significant. possible reasons for this discrepancy are discussed, together with the effect on statistical significance of a small percentage of false antigen assignments in one of the samples. a small systematic ...	1988	266606
glomus tumors with special reference to children's feet.		1988	211151
[without vocal cords].		1988	259378
[procedure for immobilization of comminuted fractures of the zygomatic arch].	the authors suggest a method for the reduction and immobilisation comminutive fractures in varus of the zygomatic arch. this procedure consists of the introduction of a franchebois balloon filled with physiological saline into the external space of the manducatory sysarcoses, thereby maintaining the calibe of the zygomato-temporo-malar canal. the procedure falls within the context of the functional treatment of fractures of the facial skeleton.	1988	266263
a proposed mechanism of action for alg and atg in severe acquired aplastic anaemia.	in this study we have shown that various batches of anti-lymphocyte globulin (alg), anti-thymocyte globulin (atg) and human intravenous immunoglobulin (iv ig) all contain antibodies with the capacity to block lymphocyte receptors for the fc region of igg, i.e., fc gamma receptors. these antibodies may exert an effect on fc gamma receptor bearing suppressor t cell function in vivo. since t cells have been implicated in the pathogenesis of acquired severe aplastic anaemia, it is conceivable that a ...	1988	2967377
[shigella serotypes and drug resistance patterns of strains isolated in etimesgut, turkey, in 1987].	drug resistance patterns of shigella strains were investigated in a prospective manner in etimesgut district during a period of 1 year. thirty strains shigella were isolated, belonged to three subgroups with preponderance of sh. flexneri (70%), followed by sh. sonnei (27%) and sh. boydii (3%). the resistance was highest with streptomycin (80%), followed by trimethoprim-sulfamethoxazole (tmp-smz) (53%) and ampicillin (43%). only three strains (10%) were sensitive to all eight antibiotics tested. ...	1988	3078794
[the possibility of using the demonstration of shigella antigens in the study of the epidemic process in dysentery].	the detection of shigella antigens in feces in accordance with the results of the passive hemagglutination test is more effective than the bacteriological method for finding out persons infected with shigella, especially in the absence of pronounced clinical manifestations of dysentery, and can be used in the study of the mechanism of the epidemic process. the examination of two groups, each consisting of permanent and temporary members, has revealed that the use of this method for detection of ...	1988	2977464
[the results of a seroepidemiologic study of newcastle dysentery].	differences in the monthly distribution in the number of seropositive individuals among children and adults in years with different intensity of the epidemic process have been revealed. immunity in cases of dysentery caused by s. newcastle reflects the yearly and seasonal activation of the epidemic process in this shigella infection both in children and adults, the seroconversion characteristics observed in the year of a high morbidity level being a more objective criterion indicating the beginn ...	1988	2977465
[experimental study of atherosclerosis. i. anti-atherosclerotic actions of chinese herb effective component 8501].		1988	2978250
genetics and regulation of enterobactin genes in shigella flexneri.	although shigella flexneri possesses the genes for two siderophore systems, enterobactin and aerobactin, the enterobactin system is only rarely utilized. to investigate the regulation of enterobactin expression in s. flexneri, all of the genes specifically required for synthesis and transport of enterobactin were cloned from both an expressing (ent+) and a nonexpressing (ent-) strain. notable differences between the cloned genes included endonuclease restriction site changes and the presence of ...	1988	2973458
[clinical characteristics of dysentery today].		1988	2973539
myosin atpase activity during avian cardiac and skeletal muscle development.	the myosin atpase activity and myosin light chain composition in developing chick heart and skeletal muscles were studied and compared. embryonic myosin was purified and characterized from day 7 to day 19 of embryogenesis. embryonic cardiac myosin generally showed the same ca2+-activated myosin atpase activity level as the adult value. in comparison, pooled pectoralis and hindlimb skeletal muscles from day 10 through day 19 showed myosin atpase activities that were all significantly less than th ...	1988	2976104
apparent hyperalgesia in the mouse tail-flick test due to increased tail skin temperature after lesioning of serotonergic pathways.	the relationship between tail skin temperature and responsiveness to noxious radiant heat in the tail-flick test was investigated in mice. a significant negative correlation between tail skin temperature and tail-flick latency was found when the tail skin temperature was increased by elevating the ambient temperature. after intracerebroventricular injection of the serotonin neurotoxin 5,7-dihydroxytryptamine (5,7-dht, 80 micrograms) tail skin temperatures were increased and tail-flick latencies ...	1988	2976242
[rpo pathway as a possible recombination mechanism of the interaction between dna of transducing phages and the escherichia coli genome].	the conditions affecting recombination of dnas of transducing lambdoid rifd phages with the chromosome of escherichia coli k-12 reca in the region of homology were studied. in support to the previously obtained data, the int system was shown to take no part in the process. the homologous character of recombination interactions and their dependence on efficiency of transcription were demonstrated. it is therefore suggested that recombination takes the rpo pathway. similar peculiarities were revea ...	1988	2976700
death despite malaria prophylaxis.		1988	3133035
processing of neurofilament proteins from perikaryal to axonal type.	in previous studies, neuronal cell bodies, excised by hand from bovine spinal ganglia, were analyzed and heterogeneous intermediate and high molecular weight neurofilament proteins that differed in electrophoretic mobility from their axonal counterparts were demonstrated. in the present experiment, intermediate and high molecular weight neurofilament proteins of the axonal type were treated with alkaline phosphatase, and neurofilament proteins enriched in perikaryal type proteins were labeled wi ...	1988	3137483
[dysenteric arthritis--a case-related observation].		1988	3140351
expression of four virulence antigens of shigella flexneri is positively regulated at the transcriptional level by the 30 kilodalton virf protein.	on the virulence plasmid of shigella flexneri the virg region required for cell-to-cell spread of the bacterium encodes a 130 kilodalton (kd) antigen and region-2 essential for the bacterial invasion of epithelial cells encodes 57, 43 and 39 kd antigens. the expression of these four antigens is positively regulated by the 30 kd protein encoded by virf, whose nucleotide sequence had been determined and which was previously found to be essential for virulence. an approximately 3.8 kilobase (kb) rn ...	1988	3141742
short- and long-term effects of a single dose of bisphosphonates on retinoid-induced bone resorption in thyroparathyroidectomized rats.	the inhibitory effect of a single subcutaneous (s.c.) dose of three different bisphosphonates (bps)--4-amino-1-hydroxybutylidene-1,1-bisphosphonate (ahbubp), 2-(2-pyridinyl)-ethylidene-1,1-bisphosphonate (2-pebp), and dichloromethylene-bisphosphonate (cl2mbp)--was studied in a model of retinoid-induced bone resorption, which consists of assessing the hypercalcemic effect of the arotinoid ro 13-6298 given s.c. for three days in thyroparathyroidectomized (tptx) rats. the retinoid was given on day ...	1988	3145796
plasmid dna survey of clinically isolated shigella strains in shanghai area.		1988	3145835
effects of maternal exposure to polychlorobiphenyls (pcbs) on f1 generation behavior in the rat.	the effect of fenclor 42 (pcb) exposure of female rats (fischer 344 strain) was studied through assessment of the behavioral development of their f1 progeny. female rats were exposed to pcb according to the following treatment schedule: (a) (5 days) 2 weeks prior to mating, (b) during gestation (days 6-15 of pregnancy), (c) during lactation (days 1-21 after delivery). behavioral endpoints of motor reflexes, motor coordination, activity (preweaning behaviors), and learning (postweaning behavior) ...	1988	3146520
scopolamine produces environment-specific conditioned activity that is not blocked by pimozide in rats.	a classical conditioning paradigm was employed to determine if the stimulant effects of the anticholinergic scopolamine could show conditioning. in experiment 1 rats had 12 60-min pairings of scopolamine (1.0 or 8.0 mg/kg, ip) with a distinctive environment that monitored horizontal and vertical activity. experimental (paired) groups received the drug 30 min prior to each session, whereas control (unpaired) groups received saline. following each session the paired groups were injected with salin ...	1988	3146771
effects of interferon alpha on performance in man: a preliminary report.	the effects of three doses of interferon alpha (1.5 mu, 0.5 mu and 0.1 mu) on performance were studied. the injection of 1.5 mu ifn produced symptoms and performance changes which closely resembled those found in volunteers with influenza. specifically, volunteers were slower at responding when they were uncertain when a target stimulus would appear, but were not impaired on a pursuit tracking task or syntactic reasoning task. the results suggest that interferon-induced changes in cns function p ...	1988	3146777
[the effect of long-term exposure to gamma rays (6,7 gy) on the activity of monoamine oxidase in the hypothalamus of sheep during the anestrus period].		1988	3146833
the influence of duration of photoperiod and hemicastration on growth and testicular and endocrine functions of boars.	yorkshire boars were used to evaluate the influence of duration of photoperiod and hemicastration on growth and testicular and endocrine functions. at 10 wk of age, 5 hemicastrate (hc) and 5 intact (i) boars were assigned to either 8 or 16 hr of light daily until 6 mo of age. body weights were recorded biweekly throughout the experiment. venous cannulae were placed in all boars at 6 mo of age, and serum was collected at 30 min intervals from 0800 to 2000 hr. gonadotropin releasing hormone (gnrh) ...	1988	3147165
carbonic anhydrase isoenzymes in the caecum and colon of normal and germ-free rats.	histochemical and immunocytochemical methods were used to study the presence of carbonic anhydrase (ca) isoenzymes in the caecum and colon of normal and germ-free rats. very high enzyme activity was demonstrated by histochemistry in the caecum and proximal colon of normal rats, while the activity decreased in the distal colon. very strong immunostaining for the isoenzyme ca i was found in the cytoplasm of surface cells and upper gland cells in the caecum and colon of normal rats. in the distal c ...	1988	3147569
in vivo measurement of intracellular ph in human brain during different tensions of carbon dioxide in arterial blood. a 31p-nmr study.	the effect of changes in carbon dioxide tension in arterial blood upon intracellular ph in brain tissue was studied in seven healthy volunteers, aged 22-45 years. the ph changes were monitored by use of 31p nuclear magnetic resonance spectroscopy, performed on a whole-body 1.5 tesla siemens imaging system. the measurements were carried out during hyperventilation and with the subject breathing atmospheric air containing 5 vol. % and 7 vol. % carbon dioxide. intracellular ph increased significant ...	1988	3147578
the development of immunological relationship between mother and fetus under physiological and pathological conditions.	on the basis of results of our research and review of literature, the complex of immuno logical influences, operating during the development of the human fetus, were evaluated. it is obvious that during the early stages of pregnancy the conceptus is protected by non-specific mechanisms, i.e. hormonally (hcg, progesterone) and by certain properties of the trophoblast (barrier function, immunologically inert surface). specific immunological tolerance is formed by gradual penetration of trophoblast ...	1988	3147589
comparison of the invasion strategies used by salmonella cholerae-suis, shigella flexneri and yersinia enterocolitica to enter cultured animal cells: endosome acidification is not required for bacterial invasion or intracellular replication.	strains of escherichia, salmonella, shigella and yersinia actively enter eukaryotic cells. several techniques were used to compare and contrast the invasion mechanisms of salmonella cholerae-suis, yersinia enterocolitica and shigella flexneri. three animal cell lines (cho, hep-2 and mdck) were examined for susceptibility to bacterial entry by these strains. levels of intracellular bacteria varied widely between cell lines, but cho cells were the most susceptible to bacterial invasion, hep-2 inva ...	1988	3147700
[lysosomal enzyme activity in the nerve tissue of various species of animals after administration of a neurotoxic pesticide aphos].		1988	3149613
production of prostaglandins and hete-compounds by isolated-cultivated bovine aortic endothelial cells.	the main product of aa conversion by baec is pgi2. other pgs were synthesized by confluent cells only in traces with exception of a hht-like compound which has still to be characterized in more details. a significant release of hete was only detected when baec were destroyed. under these conditions about 1 pmole/10(5) cells 15- and 12 hete were released by baec. taken into account the amounts of hete produced by endothelial cells as well as the partially contradictory activities of 12- and 15-he ...	1988	3150276
anti-cancer therapy with valine-depleted amino acid imbalance solution.	for the purpose of developing amino acid imbalance solution applicable to cancer treatment, we prepared seven kinds of amino acid imbalance solutions based on a 10% balanced amino acid solution and investigated the anti-tumor effects of each solution. the administration of valine-depleted amino acid solution for 8 days at a daily dose of 53 ml/rat (79.5 kcal/rat) resulted in the most significant inhibitory effects on the growth of hepatoma (ah109a) and mammary tumors (mrmt-1), the rate being 82. ...	1988	3150873
urinary infection and staghorn calculus in the left kidney.		1988	3153046
estimation of pyruvate dehydrogenase (e1) activity in human skeletal muscle; three cases with e1 deficiency.	pyruvate dehydrogenase (e1) catalyzes the rate-limiting step of the pyruvate dehydrogenase complex. since e1 activity of human muscle tissue is low, a sensitive method is needed for diagnostic purposes. measurement of 14co2 production from [1-14c]pyruvate provides a specific and sensitive assay for measuring e1 activity. we use as artificial electron acceptor dichlorophenolindophenol (dcpip) instead of the often applied ferricyanide. the method can be applied to small muscle samples obtained by ...	1988	3127087
feline aortic thromboemboli.		1988	3128904
inhibition of beta-bungarotoxin binding to brain membranes by mast cell degranulating peptide, toxin i, and ethylene glycol bis (beta-aminoethyl ether)-n,n,n',n'-tetraacetic acid.	the presynaptically active snake venom neurotoxin beta-bungarotoxin (beta-butx) is known to affect neurotransmitter release by binding to a subtype of voltage-activated k+ channels. here we show that mast cell degranulating (mcd) peptide from bee venom inhibits the binding of 125i-labeled beta-butx to chick and rat brain membranes with apparent ki values of 180 nm and 1100 nm, respectively. the mechanism of inhibition by mcd peptide is noncompetitive, as is inhibition of 125i-beta-butx binding b ...	1988	3130091
luteolytic potency of 16-phenoxy-derivatives of prostaglandin f2 alpha.	the binding of 16-phenoxy derivatives of prostaglandin (pg) f2 alpha to rat luteal membranes, and also their abortifacient potency in pregnant rats, have been studied. competitive binding studies with various pg-analogues were performed in ovaries of juvenile rats pretreated with pmsg and hcg, and in parallel studies the abortifacient potency of these substances was tested in pregnant rats. it was observed that this class of derivatives bound to the pgf2 alpha receptor as well as, or even better ...	1988	3162421
transforming growth factor-beta-induced growth inhibition and cellular hypertrophy in cultured vascular smooth muscle cells.	we have explored the hypothesis that hypertrophy of vascular smooth muscle cells may be regulated, in part, by growth inhibitory factors that alter the pattern of the growth response to serum mitogens by characterizing the effects of the potent growth inhibitor, transforming growth factor-beta (tgf-beta), on both hyperplastic and hypertrophic growth of cultured rat aortic smooth muscle cells. tgf-beta inhibited serum-induced proliferation of rat aortic smooth muscle cells (ed50 = 2 pm); this is ...	1988	3166463
a correlation between regional acetylcholinesterase activity in rat brain and performance in a spatial task.	the acquisition and retention of a water maze task was examined in 12 intact, young wistar rats. acetylcholinesterase activity in 43 discrete brain regions was then measured in the same rats by quantitative histochemistry. individual learning and retention indices were found to be significantly correlated with acetylcholinesterase (ache) levels in specific regions, e.g. cholinergic nuclei; the ventral pallidum and nucleus basalis; and in the dentate gyrus of the hippocampus. high levels of ache ...	1988	3166718
changes in vascular reactivity in experimental diabetic rats: comparison with hypothyroid rats.	the responsiveness to vasoactive agents in the perfused mesenteric vascular bed of streptozocin-induced diabetic rats was examined and compared with that of propylthiouracil-induced hypothyroid rats. diabetic rats at 4 and 8 weeks after the induction of diabetes showed a significant decrease in isoproterenol-induced vasodilatation. in addition, the contractile responses to norepinephrine and 5-hydroxytryptamine and the vasodilative response to acetylcholine were significantly decreased in 12-wee ...	1988	3167224
production of tumor necrosis factor in nude mice by muramyl peptides associated with bacterial vaccines.	gram-negative vaccines can elicit the production of tumor necrosis factor (tnf) in mice primed by muramyl dipeptide (mdp) or by its lipophilic derivative mdp-dipalmitoyl glycerol (mdp-gdp). in mice pretreated with mdp and particularly with mdp-gdp, bordetella pertussis vaccine was shown to be more effective than typhoid vaccine. the time course of tnf production in the blood did not indicate any difference between the effect of mdp or of mdp-gdp. in both cases the cytotoxic activity reached maxi ...	1988	3167834
production of human-human hybridomas secreting monoclonal antibodies reactive to breast cancer cell lines.	we produced human-human hybridomas by fusing lymph node lymphocytes of breast cancer patients with a fusion partner, ho-323 cells, in the presence of 50% polyethylene glycol, and screened hybridomas producing monoclonal antibody (moab) reactive to a breast cancer cell line, mcf-7. among 11 hybridomas secreting igm reactive to mcf-7, four hybridomas (h15a3, h15f2, k15b4, and k15c3) produced igm reacting specifically to breast cancer cell lines (mcf-7 and hbc-5). among them, h15a3 proliferated in ...	1988	3170451
effect of vagotomy on bile acid absorption by rat small intestine in vitro.		1988	3175250
multiple cysteine proteinase forms during the life cycle of dictyostelium discoideum revealed by electrophoretic analysis.	proteinases of the cellular slime mould dictyostelium discoideum have been analysed using electrophoresis on polyacrylamide gels containing gelatin (gelatin/page). multiple proteinase forms were apparent in vegetative myxamoebae, but the presence of individual enzyme forms depended on the manner in which the cells were grown. axenic cells had a characteristic a-pattern of proteinases consisting of six bands, the most active enzymes having apparent mr values of 51,000 and 45,000 (these have been ...	1988	3178750
activity of glucosidase inhibitors against hiv infections.		1988	3182426
heterogeneous colony forming ability in vitro of the abnormal clone-derived granulocyte-macrophage precursors in myelodysplastic syndromes.	the clonal origin of granulocyte-macrophage colony forming cells (cfu-gm) in the myelodysplastic syndrome (mds) was cytogenetically studied. chromosome analysis was carried out on single gm-colonies from six patients with mds, whose bone marrow cells had chromosome abnormalities. abnormal clone-derived cfu-gm were grown in four patients under the presence of human placental conditioned medium. in the remaining two, all analysed colonies revealed a normal karyotype, although the majority of metap ...	1988	3184986
measurement of local cerebral glucose utilization before and after v-p shunt in congenital hydrocephalus in rats.	local cerebral glucose utilization (lcgu) was measured by the [14c]deoxyglucose autoradiographic method in rats with congenital hydrocephalus before and after a v-p shunt. lcgu was decreased throughout the brain in hydrocephalus. the most severely affected regions were found in the cortex, with which a marked degree of subcortical cerebrospinal fluid (csf) edema was associated; regions in the brain-stem structures were less affected. these metabolic disturbances were improved in many regions aft ...	1988	3185424
thoracic cord compression in scheuermann's disease.	a 29-year-old man with spastic paraparesis of insidious onset was discovered to have thoracic cord compression by a combination of kyphosis and thoracic disc herniation. radiologic evaluation showed evidence of scheuermann's disease, of which thoracic kyphosis is one manifestation. neurologic signs are uncommon in scheuermann's disease and few cases have been successfully managed with resolution of neurologic deficit. this patient made a virtually complete recovery after thoracic cord decompress ...	1988	3187711
progressive ankylosis in mice. an animal model of spondylarthropathy. i. clinical and radiographic findings.	to determine its similarity to human spondylarthropathies, we studied murine progressive ankylosis, a spontaneously occurring disorder of joints in mice. clinically, peripheral joints were inflamed initially, then became ankylosed in a predictable sequence from distal to proximal. forefeet were involved before hindfeet. axial joint involvement produced severe spinal ankylosis. extraarticular manifestations included balanitis and crusting skin lesions. radiographically, bony erosions and calcific ...	1988	3190783
characterization of two deletions that remove the entire human zeta-alpha globin gene complex (- -thai and - -fil).	we have fully characterized two alpha thalassaemia mutants that occur in southeast asia, - -thai and - -fil. each mutant is due to a deletion that removes the entire zeta-alpha-globin gene complex. localization of the 5' breakpoints described here, allows the identification of unique fragments that are specific for each of the two mutations. this information can be used to assess the frequency of these mutants in southeast asia and will be of value in prenatal testing for alpha thalassaemia in t ...	1988	3191033
[effect of the induction of liver cytochrome p-450 by a chemically inert fluorocarbon on the reactions of immediate and delayed hypersensitivity].	the effect of chemically inert perfluorodecalin (pfd) on the development of hypersensitive reactions of immediate and delayed types in guinea pigs and mice was studied. it was found that administration of pfd to animals both before sensitization (mice) and during the development of sensitization (guinea pigs) reduced the degree of the both types of hypersensitivity.	1988	3191983
mobile sequences in the pyruvate dehydrogenase complex, the e2 component, the catalytic domain and the 2-oxoglutarate dehydrogenase complex of azotobacter vinelandii, as detected by 600 mhz 1h-nmr spectroscopy.	600 mhz 1h-nmr spectroscopy demonstrates that the pyruvate dehydrogenase complex of azotobacter vinelandii contains regions of the polypeptide chain with intramolecular mobility. this mobility is located in the e2 component and can probably be ascribed to alanine-proline-rich regions that link the lipoyl subdomains to each other as well as to the e1 and e3 binding domain. in the catalytic domain of e2, which is thought to form a compact, rigid core, also conformational flexibility is observed. i ...	1988	3191993
the disposition of [2,3-14c]-methyl and [2,3-14c]-2-ethylhexyl acrylate in male wistar albino rats.	the disposition of methyl [2,3-14c]-acrylate (ma) and 2-ethylhexyl [2,3-14c]-acrylate (eha) following intraperitoneal and oral administration to rats has been studied. the 14c found in the tissues was mainly associated with liver, kidneys and lungs. loss of 14c from these tissues occurred fairly rapidly, excluding the rats given eha intraperitoneally. most of the administered acrylates underwent rapid metabolism and excretion with expired air (more than 50% of the dose and urine (10-50% of the d ...	1988	3196152
dog owners and their use of veterinary services.		1988	3198454
phaeohyphomycosis caused by alternaria alternata in a cat.	phaeohyphomycosis caused by alternaria alternata was diagnosed in a 6-year-old cat. a lesion in the nose resulted in enlargement of the dorsum of the nose. similar appearing lesions had been removed from the dorsum of the nose 1 and 4 years earlier. the lesion recurred 3 months after surgical excision and irregular administration of ketoconazole. a second cytoreductive operation followed by 5 months' treatment with ketoconazole resolved the infection. nasal trauma occurring at 8 months and at 5 ...	1988	3198465
a novel 190 kda calmodulin-binding protein associated with brain actomyosin.	we present evidence for a novel calmodulin-binding protein associated with ca2+-sensitive, non-muscle actomyosin from rabbit brain. gel filtration chromatography of brain actomyosin and sds-page analysis of resulting fractions revealed the presence of a stable complex of a 190 kda polypeptide and calmodulin. furthermore, brain actomyosin was retained on a column of conjugated calmodulin-sepharose 4b and eluted by 6 m urea in the presence of ca2+. further elution with 6m urea plus egta released t ...	1988	3203157
[ontogenetic characteristics of ascorbic acid binding by nerve tissue proteins].	the highest ascorbic acid content has been found in a low-molecular fraction (m. w. less than 6000) of water-soluble proteins from brain tissue homogenates of rats, the lowest one--in a high-molecular fraction (m. w. about 65,000). the content of protein-bound ascorbic acid decreases during postnatal life. this process is accompanied by changes in the ratio of the reduced and oxidated forms of the ascorbic acid in favour of the latter.	1988	3206951
connective tissue remodelling in the ovine cervix during pregnancy and at term.	cervical ripening and dilation in the ewe has been quantitated in terms of the connective tissue content of the tissue and the biosynthesis of the macromolecular components which make up the extracellular matrix of this tissue. during pregnancy there is a marked increase in the total tissue mass of the cervix along with the total mass of hydroxyproline (collagen) and hexuronate (proteoglycans and hyaluronate). the concentration of hydroxyproline of the cervix based on wet weight of the tissue de ...	1988	3208512
connective tissue remodelling in the ovine cervix during pregnancy and at term.	cervical ripening and dilation in the ewe has been quantitated in terms of the connective tissue content of the tissue and the biosynthesis of the macromolecular components which make up the extracellular matrix of this tissue. during pregnancy there is a marked increase in the total tissue mass of the cervix along with the total mass of hydroxyproline (collagen) and hexuronate (proteoglycans and hyaluronate). the concentration of hydroxyproline of the cervix based on wet weight of the tissue de ...	1988	3208512
paracrine control of immature sertoli cells by adult germ cells, in the rat (an in vitro study). cell-cell interactions within the testis.	enriched populations of germ cells prepared from adult rats were found to influence 20-day-old rat sertoli cell secretory activity by stimulating androgen-binding protein (abp) and inhibiting oestradiol-17 beta production in the presence of follicle-stimulating hormone (fsh) as well as of dibutyryl cyclic amp (dbcamp). among the different populations tested in coculture, pachytene spermatocytes were the most effective at stimulating abp and inhibiting oestradiol production, whereas early spermat ...	1988	3208988
gut-associated lymphoid tissue in the large intestine of calves. ii. electron microscopy.	scanning electron microscopy of lymphoid tissue in the large intestine of three germfree calves (age 3, 6, and 7 days) revealed two different units: propria nodules and lymphoglandular complexes (lgc). propria nodules had lymphoid tissue predominantly in lamina propria and were covered by distinct follicle-associated epithelium which lacked goblet cells; nodules were surrounded by wide crypts, which were also lined by follicle-associated epithelium towards the luminal side. lymphoglandular compl ...	1988	3212895
[facilitatory effect of trifluoperazine on adrenergic transmitter release from rat portal vein].		1988	3218532
arautp-affi-gel 10: a novel affinity absorbent for the specific purification of dna polymerase alpha-primase.	for the specific purification of eukaryotic dna-dependent dna polymerase alpha, we prepared two novel affinity resins bearing 5-(e)-(4-aminostyryl) arautp as a ligand. one of them was arautp-sepharose 4b which was coupled directly with the ligand and the other was arautp-affi-gel 10 which was coupled with the ligand through a spacer. no dna polymerase alpha-primase activity from cherry salmon (oncorhynchus masou) testes was bound on the arautp-sepharose 4b in all cases examined. on the other han ...	1988	3218743
affinity of microorganisms of the genus ureaplasma to the reproductive organs of cattle.	the purpose of this work was to define more precisely the role of ureaplasma organisms in the aetiology of granular vulvovaginitis and balanoposthitis (gvvbp) of cattle. to contribute to this question the frequency and degree of infection with ureaplasmas in two main groups of cattle was taken into account: (a) in cattle with symptoms of the mentioned disease, (b) in cattle without clinical symptoms. the samples of semen from 301 sires with symptoms of gvvbp and from 43 healthy sires as also vag ...	1988	3219829
epidemiology of hiv infection in africa.	hiv infection in africa is primarily acquired through heterosexual activity, accounting for up to 80% of cases. prostitutes and sexually promiscuous individuals are at particularly high risk of acquiring infection via this route. in the general population, women between the ages of 18 and 30 years are at increased risk of transmission. the role of cofactors, particularly concurrent sexually transmitted diseases (stds), appears to facilitate heterosexual spread. these groups represent opportuniti ...	1988	3225742
age and lpr dependent induction of increased sensitivity to the toxic effects of lipopolysaccharide and indomethacin in mrl mice: evidence for res activation during disease progression.	injection of 4.5-6 month old female mrl-lpr/lpr mice with 200-250 micrograms lipopolysaccharide led to the death of the animals within two days. treatment of age matched female control mice (mrl-+/+) or young (3 month) mrl-lpr/lpr mice did not lead to similar toxicity. however, pretreatment of young mice with the pyridine extract of residue of corynebacterium parvum (alternatively designated propionibacterium acnes) prior to lipopolysaccharide injection, led to the rapid death of 100% of the mic ...	1988	3230580
[the genetic activity of an alkaline solution of formaldehyde].	toxicity and genetic activity of disinfectant consisting of 1.5% paraform and 0.5% naoh were studied. it was found that the preparation caused a weak mutagenic effect on crepis capillaris and chlorella roots, but had no effect in the ames test and gonadal cells of mammals. the studies of mutagenic effect of the preparation on somatic cells of white mice show limits of the cytogenetic effect at the level of 3.5 g/kg (1/10 ld50).	1988	3232201