Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| chronic infection with camelid hepatitis e virus in a liver transplant recipient who regularly consumes camel meat and milk. | there have been increasing reports of food-borne zoonotic transmission of hepatitis e virus (hev) genotype 3, which causes chronic infections in immunosuppressed patients. we performed phylogenetic analyses of the hev sequence (partial and full-length) from 1 patient from the middle east who underwent liver transplantation, and compared it with other orthohepevirus a sequences. we found the patient to be infected by camelid hev. this patient regularly consumed camel meat and milk, therefore came ... | 2016 | 26551551 |
| serologic and molecular survey of hepatitis e virus in german deer populations. | hepatitis e virus (hev) is a human pathogen that is primarily transmitted by the fecal-oral route and causes a usually self-limiting acute viral hepatitis. the virus is endemic in developing countries of africa, asia, and latin america and is responsible for sporadic cases in industrialized countries. in western europe, an increasing number of autochthonous cases have been associated with zoonotic transmissions of hev from domestic and wild animals. in germany, animal reservoirs for hev have bee ... | 2016 | 26528571 |
| incidence and duration of hepatitis e virus infection in dutch blood donors. | the incidence of hepatitis e virus (hev) infection in the netherlands is high. blood donors are not routinely screened for hev infection, but since january 2013, donations used for the production of solvent/detergent (s/d)-treated plasma have been screened for hev rna. | 2016 | 26559806 |
| role of asparagine at position 562 in dimerization and immunogenicity of the hepatitis e virus capsid protein. | the hepatitis e virus (hev) capsid protein, porf2, contains 2 potential n-glycosylation sites, n137 and n310, located in the s domain, and one site, n562, in the p domain. the last domain located at positions 454-606 aa forms a protruding spike from the shell, with n562 being located in the apical center of the spike, which is also a cell-attachment region and neutralizing antigenic site. here, we expressed in pichia pastoris a recombinant polypeptide p179 comprising the region of 439-617 aa of ... | 2016 | 26584510 |
| the association of cytokines and micronutrients with hepatitis e virus infection during pregnancy and the postpartum period in rural bangladesh. | hepatitis e virus (hev) infection is severe during pregnancy, with a pregnant case fatality rate around 30%. in bangladesh, plasma samples from 1,100 women during the first trimester (tm) and third tm of pregnancy and 3 months postpartum (pp) were tested for anti-hev igg. during this time, 40 women developed antibody responses to hev. these seroconverters are classified as the cases (incidence = 46 infections per 1,000 person-years). all except one seroconversion occurred between the third tm an ... | 2016 | 26621563 |
| stem cell-derived hepatocytes: a novel model for hepatitis e virus replication. | yearly, approximately 20million people become infected with the hepatitis e virus (hev) resulting in over 3million cases of acute hepatitis. although hev-mediated hepatitis is usually self-limiting, severe cases of fulminant hepatitis as well as chronic infections have been reported, resulting annually in an estimated 60,000 deaths. we studied whether pluripotent stem cell (psc)-derived hepatocytes, mesodermal and/or neuroprogenitor cells support hev replication. | 2016 | 26626494 |
| acute and chronic hepatitis e virus infection in human immunodeficiency virus-infected u.s. women. | exposure to hepatitis e virus (hev) is common in the united states, but there are few data on prevalence of hev/human immunodeficiency virus (hiv) coinfection in u.s. | 2016 | 26646162 |
| hepatitis e virus orf1 encoded non structural protein-host protein interaction network. | hepatitis e virus orf1 encoded non-structural polyprotein (nsp) consist of multiple domains, namely: methyltransferase, y-domain, protease, x-domain, helicase and rna dependent rna polymerase. we have attempted to identify human liver cell proteins that are interacting with hev orf1 encoded functional domains by using y2h screening. a total of 155 protein-protein interactions between hev-orf1 and human proteins were identified. comparative analysis of the hev-orf1-human interaction network with ... | 2016 | 26689634 |
| hepatitis e virus genotype 3 infection in a tertiary referral center in the netherlands: clinical relevance and impact on patient morbidity. | hepatitis e virus (hev) genotype 3 infections can have important clinical consequences. | 2016 | 26687439 |
| hepatitis e virus-induced cryoglobulinemic glomerulonephritis in a nonimmunocompromised person. | hepatitis e virus (hev)-related kidney disease and symptomatic cryoglobulinemia have been observed in solid-organ transplant recipients. however, hev rna in the cryoprecipitate has not yet been assessed. we report what to our knowledge is the first documented case of autochthonous hev-induced cryoglobulinemic crescentic and membranoproliferative glomerulonephritis in an immunocompetent man with no notable medical history. he presented with edema, hypertension, increased serum creatinine level, a ... | 2016 | 26682764 |
| diagnostic performance of five assays for anti-hepatitis e virus igg and igm in a large cohort study. | determination of anti-hepatitis e virus (anti-hev) antibodies is still enigmatic. there is no gold standard, and results obtained with different assays often diverge. herein, five assays were compared for detection of anti-hev igm and igg. serum samples from 500 swedish blood donors and 316 patients, of whom 136 had suspected hev infection, were analyzed. concordant results for igm and igg with all assays were obtained only for 71% and 70% of patients with suspected hepatitis e, respectively. th ... | 2016 | 26659210 |
| coastal clustering of hev; cornwall, uk. | autochthonous hepatitis e virus (hev) infection is a porcine zoonosis and increasingly recognized in developed countries. in most cases the route of infection is uncertain. a previous study showed that hev was associated geographically with pig farms and coastal areas. | 2016 | 26709884 |
| seroprevalence of hepatitis e virus in autoimmune hepatitis patients in the netherlands. | in recent years chronic courses of hepatitis e virus (hev) infection have been described in immunosuppressed individuals. this may implicate a potential role for hev in the development of autoimmune diseases, including autoimmune hepatitis (aih). here we investigated the prevalence of hev-antibodies in aih patients in an endemic central european country. | 2016 | 27014749 |
| hepatitis e virus infection among solid organ transplant recipients at a north american transplant center. | background. autochthonous hepatitis e virus (hev) infection has been reported in over 200 solid organ transplant (sot) recipients since 2006, yet little is known about the burden of hev among sot recipients in north america. we performed a retrospective, cross-sectional study to investigate the prevalence and risk factors associated with hev infection among sot recipients at our institution. methods. children and adults (n = 311) who received allografts between 1988 and 2012 at the johns hopki ... | 2016 | 27014710 |
| concomitant outbreaks of yellow fever and hepatitis e virus in darfur states, sudan, 2012. | yellow fever (yf) is a vector-borne disease transmitted to humans by infected aedes mosquitoes, while hepatitis e virus (hev) is a waterborne disease that is transmitted through the fecal-oral route. both diseases have very close clinical presentation, namely fever, jaundice, malaise, and dark urine; they differ in severity and outcome. | 2016 | 26829534 |
| hepatitis e virus infection among blood donors in the south caribbean: is screening warranted? | 2016 | 26819367 | |
| no evidence of genotype-3 hepatitis e virus-induced myocarditis. | 2016 | 26815640 | |
| hepatitis e virus infection in the liver transplant recipients: clinical presentation and management. | hepatitis e virus (hev) is an emerging pathogen and an increasingly recognized cause of graft hepatitis, especially in the post-orthotopic liver transplantation immunocompromised population. the exact incidence and prevalence of hev infection in this population remains unclear but is certainly greater than historical estimates. identifying acute hev infection in this population is imperative for choosing the right course of management as it is very difficult to distinguish histologically from ac ... | 2016 | 26807207 |
| human liver chimeric mice as a new model of chronic hepatitis e virus infection and preclinical drug evaluation. | hepatitis e virus (hev) is a major cause of acute hepatitis as well as chronic infection in immunocompromised individuals; however, in vivo infection models are limited. the aim of this study was to establish a small animal model to improve our understanding of hev replication mechanisms and permit the development of effective therapeutics. | 2016 | 26805671 |
| ferret hepatitis e virus infection induces acute hepatitis and persistent infection in ferrets. | ferret hepatitis e virus (hev), a novel hepatitis e virus, has been identified in ferrets. however, the pathogenicity of ferret hev remains unclear. in the present study, we compared the hev rna-positivity rates and alanine aminotransferase (alt) levels of 63 ferrets between before and after import from the us to japan. we found that the ferret hev-rna positivity rates were increased from 12.7% (8/63) to 60.3% (38/63), and alt elevation was observed in 65.8% (25/38) of the ferret hev rna-positiv ... | 2016 | 26790932 |
| chemically activatable viral capsid functionalized for cancer targeting. | to design a theranostic capsule using the virus-like nanoparticle of the hepatitis e virus modified to display breast cancer cell targeting functional group (lxy30). | 2016 | 26786134 |
| full coding hepatitis e virus genotype 3 genome amplification method. | hepatitis e virus (hev) genotype 3 produces zoonotic infection associated with the consumption of infected animals. hev infections can become chronic in immunocompromised (ic) patients. the viral genome has three well defined open reading frames (orf1, orf2 and orf3) within which various domains and functions have been described. this paper (i) describes a new method of complete sequencing of the hev coding region through overlapping pcr systems, (ii) establishes a consensus sequence and polymor ... | 2016 | 26784284 |
| a trivalent vaccine candidate against hepatitis e virus, norovirus, and astrovirus. | hepatitis e virus (hev), norovirus (nov), and astrovirus (astv) are enterically-transmitted viral pathogens causing epidemic or endemic hepatitis (hev) and gastroenteritis (nov and astv) respectively in humans, leading to significant morbidity and mortality worldwide. while a recombinant subunit vaccine against hevs is available in china, there is no commercial vaccine or antiviral against nov or astv. we report here our development of a trivalent vaccine against the three viral pathogens throug ... | 2016 | 26778421 |
| seroepidemiology of hepatitis e in selected population groups in croatia: a prospective pilot study. | hepatitis e has become an emerging infection in many european countries. we analysed the prevalence of hepatitis e virus (hev) infection in selected population groups in croatia. overall hev igg seropositivity was 5.6%, while 1.9% participants showed igm antibodies suggestive of recent infection. no igm-positive sample was positive for hev rna. hev igg antibodies were most prevalent in alcohol abusers (8.9%) and war veterans (8.6%), compared with 6.1% among injecting drug users and 2.7% in healt ... | 2016 | 26776465 |
| prophylaxis against hepatitis e: at risk populations and human vaccines. | hepatitis e is an emerging global disease caused by hepatitis e virus (hev) infection. while in developing countries the infection was primarily due to poor sanitary conditions through intake of contaminated water or undercooked meats of infected animals, increasing cases of chronic hepatitis e resulting in rapidly progressive liver cirrhosis and end-stage liver disease have been reported in organ transplant patients or in immune compromised patients in developed countries. fortunately, hepatiti ... | 2016 | 26775537 |
| a public health initiative on hepatitis e virus epidemiology, safety and control in portugal--study protocol. | the discovery of autochthonous hepatitis e in industrialized countries has changed the understanding of hepatitis e virus (hev) infection in these regions, now known to be mainly due to zoonotic transmission of genotype 3. the foodborne route of transmission via consumption of contaminated meat from hev infected pigs is well documented as well as the direct occupational exposure to animal reservoirs. accumulating evidence also points to an emerging potential threat to blood safety after the iden ... | 2016 | 26774897 |
| orf3 of hepatitis e virus inhibits the expression of proinflammatory cytokines and chemotactic factors in lps-stimulated human pma-thp1 cells by inhibiting nf-κb pathway. | hepatitis e virus (hev) is one of the primary causative agents of acute hepatitis. it is noteworthy that hev can develop chronic infection and even lead to liver cirrhosis; however, the mechanism has not been revealed. in this study, the elisa assay was used to detect protein levels, and we found that hev open reading frame 3 (orf3) protein inhibited the expression of proinflammatory cytokines (tumor necrosis factor-alpha [tnf-α], interleukin [il]-1β, il-6, il-8, il-12p40, and il-18) and chemota ... | 2016 | 26771290 |
| production of infectious ferret hepatitis e virus in a human hepatocarcinoma cell line plc/prf/5. | a strain of ferret hepatitis e virus (hev), sf4370, isolated from an imported ferret was used to inoculate a human hepatocarcinoma cell line, plc/prf/5. the virus genome and capsid protein were detected in the cell culture supernatant. immunofluorescence microscopy indicated that the capsid protein was located in the cytoplasm. the virus particles were purified from the culture supernatant by sucrose gradient ultracentrifugation. the capsid protein with molecular mass of ∼72 kda was detected in ... | 2016 | 26763355 |
| prevalence of anti-hepatitis e virus antibodies and first detection of hepatitis e virus in wild boar in slovenia. | hepatitis e is an emerging zoonotic disease caused by hepatitis e virus (hev). in this study, we investigated hev presence in a wild boar (sus scrofa) population of slovenia. a total of 288 wild boar serum samples were collected throughout the country, and hev infection was investigated by serology, using enzyme-linked immunosorbent assay (elisa) and by hev rna detection using a real-time pcr assay. antibodies against hev were detected in 30.2% (87/288) of animals tested, whereas hev rna was det ... | 2016 | 26757050 |
| hepatitis e virus outbreak in postearthquake nepal: is a vaccine really needed? | 2016 | 26756604 | |
| hepatitis e virus seroprevalence in free-ranging deer in canada. | hepatitis e virus infection (hev) is an important public health concern not only in traditional endemic areas, but also in some industrialized countries where both domesticated and wild animals have been recognized as potential zoonotic reservoirs implicated in hev transmission. while the prevalence of infection in the deer population in europe and asia has been thoroughly investigated, it remains largely undetermined in north america. we assessed the presence of hev in three different species o ... | 2016 | 26752436 |
| simulation of viral hepatitis e in marmosets. | we developed a model of hepatitis e virus infection in common marmosets (callithrix jacchus) and determined optimal route of infection, duration, clinical and virological characteristics of infection in laboratory animals. using this model, we demonstrated that replication of hepatitis e virus primarily occurs in the liver, while virus replication presumed to take place in the intestine was not confirmed in this experiment. | 2016 | 26750931 |
| proposed reference sequences for hepatitis e virus subtypes. | the nomenclature of hepatitis e virus (hev) subtypes is inconsistent and makes comparison of different studies problematic. we have provided a table of proposed complete genome reference sequences for each subtype. the criteria for subtype assignment vary between different genotypes and methodologies, and so a conservative pragmatic approach has been favoured. updates to this table will be posted on the international committee on taxonomy of viruses website (http://talk.ictvonline.org/r.ashx?c). ... | 2016 | 26743685 |
| seroprevalence of hepatitis e virus infection among dogs in several developed cities in the guangdong province of china. | hepatitis e virus (hev), as a zoonotic disease virus, was seldom studied in dogs especially in stray dogs. as previously reported, dog might be an accidental host of hev for human beings and some risk factors might play an important role in hev transmission. thus, we designed this study to evaluate the seroprevalence of hev infection among dogs in several cities in guangdong province of china. this surveillance may help us understand risk factors including location, gender, live type, and diet h ... | 2016 | 26743155 |
| development and evaluation of a rt-lamp assay for rapid detection of hepatitis e virus from shellfish. | hepatitis e virus (hev) has becoming a well known zoonotic enteric pathogen and circulated widely inter human-animal-water-food. generally, detection of the virus has relied on conventional reverse transcription-pcr (rt-pcr) and taqman/sybr quantitative real-time rt-pcr (rt-qpcr), but these tools are usually disadvantages in time-consuming and expensive instruments required. in the present study, we report here on the development of a one-step single-tube reverse transcription-loop-mediated isot ... | 2016 | 26741532 |
| hepatic failure after pig heart transplantation into a baboon: no involvement of porcine hepatitis e virus. | after transplantation of pig hearts into baboons, a particularly high increase of liver parameters was observed in 1 animal. to evaluate whether porcine hepatitis e virus (hev) was involved in the pathological changes, the donor pig and the recipient baboon were screened for the presence of hev. | 2016 | 26739324 |
| when should ribavirin be started to treat hepatitis e virus infection in transplant patients? | 2016 | 26729540 | |
| hepatitis e, the neglected one. | hepatitis e virus (hev) infection is a worldwide disease. it is the first cause of acute viral hepatitis in the world with an estimated 20 million cases every year and 56 000 deaths. in developing countries, hepatitis e is a waterborne infection. in these countries, hev genotypes 1 and 2 cause large outbreaks and affect young subjects with a significant mortality rate in pregnant women and patients with cirrhosis. in the developed countries, hev genotypes 3 and 4 are responsible for autochthonou ... | 2016 | 26725910 |
| antigen detection and apoptosis in mongolian gerbil's kidney experimentally intraperitoneally infected by swine hepatitis e virus. | we examined the effect of hepatitis e virus (hev) on the renal tissue pathogenesis, morphological damages and related molecular mechanisms following swine hev suspension intraperitoneally inoculation in mongolian gerbils. the microscopic and ultramicroscopic analyses of kidney tissue structure were carried out at different points after inoculation of hev. the immunohistochemistry, real-time pcr and western blot were performed to explore the molecular mechanisms associated with hev presence in th ... | 2016 | 26724751 |
| hepatitis e virus and neurological injury. | hepatitis e is hyperendemic in many developing countries in asia and africa, and is caused by hepatitis e virus (hev) genotypes 1 and 2, which are spread via the faecal-oral route by contaminated water. recent data show that hev infection is also endemic in developed countries. in such geographical settings, hepatitis e is caused by hev genotypes 3 and 4, and is mainly a porcine zoonosis. in a minority of cases, hev causes acute and chronic hepatitis, but infection is commonly asymptomatic or un ... | 2016 | 26711839 |
| acute hepatitis e virus infection in human immunodeficiency virus-positive men and women in nepal: not quite a rare entity. | 2016 | 26845252 | |
| virology, serology, and demography of hepatitis e viremic blood donors in south east england. | hepatitis e virus (hev) genotype 3 (g3) in england comprises two principal phylogenetic groups (group 1 and group 2) and can be transmitted by transfusion. unselected screening identified 79 viremic donors; 76 participated in a follow-up study. | 2016 | 26841005 |
| hepatitis e in transplantation. | hepatitis e virus (hev) has a worldwide distribution and is known to cause acute and fulminant hepatitis. however, over the last few years, it has been shown to also cause chronic hepatitis and cirrhosis in immunosuppressed patients, especially solid-organ-transplant patients. in immunocompetent and immunosuppressed patients, hev is also associated with extra-hepatic manifestations, such as neurological symptoms and kidney injury. unfortunately, a diagnostic assay for hev infection is still not ... | 2016 | 26838163 |
| distinct entry mechanisms for nonenveloped and quasi-enveloped hepatitis e viruses. | the hepatitis e virus (hev) sheds into feces as nonenveloped virions but circulates in the blood in a membrane-associated, quasi-enveloped form (ehev). since the ehev virions lack viral proteins on the surface, we investigated the entry mechanism for ehev. we found that compared to nonenveloped hev virions, ehev attachment to the cell was much less efficient, requiring a longer inoculation time to reach its maximal infectivity. a survey of cellular internalization pathways identified clathrin-me ... | 2016 | 26865708 |
| investigation of suspected viral hepatitis outbreaks in north west india. | hepatitis e (hev) infection is diagnosed on the basis of serum anti-hev igm detection. in outbreaks, early diagnostic method is important for prompt control measures. this study compared 3 diagnostic methods in 60 serum samples collected in suspected hev outbreaks. the suitability of saliva samples for antibody detection was also evaluated in 21 paired serum saliva samples. the anti-hev igm, hev-ag, and hev-rna were detected in serum samples of 52 (86.66%), 16 (26.66%), and 18 (30%) patients, re ... | 2016 | 26853491 |
| cross-genotype-specific t-cell responses in acute hepatitis e virus (hev) infection. | hepatitis e is an inflammatory liver disease caused by infection with the hepatitis e virus (hev). in tropical regions, hev is highly endemic and predominantly mediated by hev genotypes 1 and 2 with >3 million symptomatic cases per year and around 70 000 deaths. in europe and america, the zoonotic hev genotypes 3 and 4 have been reported with continues increasing new infections per year. so far, little is known about t-cell responses during acute hev genotype 3 infection. therefore, we did a com ... | 2016 | 26852892 |
| immunogenicity difference between two hepatitis e vaccines derived from genotype 1 and 4. | we investigated the immunogenicity difference between hepatitis e vaccine p239 derived from hepatitis e virus (hev) genotype 1 and vaccine p179 derived from hev genotype 4; and the presence of genotype-specific neutralizing epitopes. hev orf2 recombinant proteins (p166w01, p166mex, p166us and p166chn) derived from the four hev genotypes were used to detect anti-hev iggs in sera of mice and humans vaccinated with p179 or p239 and in sera of rhesus monkey challenged with hev genotype 1 or 4 strain ... | 2016 | 26850829 |
| hepatitis e virus: current epidemiology and vaccine. | hepatitis e virus infections have been continuously reported in indian subcontinent, africa, southeast and central asia, posing great health threats to the public, especially to pregnant women. hecolin® is the only licensed hev vaccine developed by xiamen innovax biotech co., ltd. extensive characterizations on antigenicity, physicochemical properties, efficacy in clinical trials, and manufacturing capability have made hecolin® a promising vaccine for hev control. however, there are many obstacl ... | 2016 | 27184971 |
| blood-borne hepatitis e virus transmission: a relevant risk for immunosuppressed patients. | 2016 | 27178472 | |
| hepatitis e virus mutations associated with ribavirin treatment failure result in altered viral fitness and ribavirin sensitivity. | ribavirin monotherapy is the preferred treatment for chronic hepatitis e, although occasional treatment failure occurs. we present a patient with chronic hepatitis e experiencing ribavirin treatment failure with a completely resistant phenotype. we aimed to identify viral mutations associated with treatment failure and explore the underlying mechanisms. | 2016 | 27174035 |
| endoplasmic reticulum stress induced synthesis of a novel viral factor mediates efficient replication of genotype-1 hepatitis e virus. | hepatitis e virus (hev) causes acute hepatitis in many parts of the world including asia, africa and latin america. though self-limiting in normal individuals, it results in ~30% mortality in infected pregnant women. it has also been reported to cause acute and chronic hepatitis in organ transplant patients. of the seven viral genotypes, genotype-1 virus infects humans and is a major public health concern in south asian countries. sporadic cases of genotype-3 and 4 infection in human and animals ... | 2016 | 27035822 |
| isolation of hepatitis e virus from breast milk during acute infection. | 2016 | 27025819 | |
| a novel blocking elisa for detection of antibodies against hepatitis e virus in domestic pigs. | hepatitis e virus (hev) infects both humans and animals, with an overall human mortality rate generally less than 1%, but as high as 20% among pregnant women. hev strains fall into 4 major genotypes. zoonotic genotypes 3 and 4 associate with sporadic human and animal hev cases in many industrialized countries. to date, collective evidence implicates pigs as the main hev reservoir, justifying the importance of monitoring hev infection rates in pig herds to prevent human illness. due to the lack o ... | 2016 | 27023902 |
| analysis of adaptive mutations selected during the consecutive passages of hepatitis e virus produced from an infectious cdna clone. | to characterize the genomic mutations of hepatitis e virus (hev) during consecutive passages associated with adaptation to growth in cell culture, a cloned genotype 3 hev [pje03-1760f/wt, starting virus (sv)] was passaged 10 times in a549 cells, and the entire genomic sequence of the passage 10 (p10) progeny was determined. compared to sv, p10 virus possessed two non-synonymous (t2808c and a5054g) and four synonymous mutations (c1213t, t2557c, c3118t and c4435t) in the orf1. full-length infectio ... | 2016 | 27485920 |
| hepatitis e virus infections in travellers: assessing the threat to the australian blood supply. | in many developed countries hepatitis e virus (hev) infections have occurred predominantly in travellers to countries endemic for hev. hev is a potential threat to blood safety as the virus is transfusion-transmissible. to minimise this risk in australia, individuals diagnosed with hev are deferred. malarial deferrals, when donors are restricted from donating fresh blood components following travel to an area in which malaria is endemic, probably also decrease the hev risk, by deferring donors w ... | 2016 | 27483488 |
| a rat model for hepatitis e virus. | hepatitis e virus (hev) is one of the prime causes of acute viral hepatitis, and chronic hepatitis e is increasingly recognized as an important problem in the transplant setting. nevertheless, the fundamental understanding of the biology of hev replication is limited and there are few therapeutic options. the development of such therapies is partially hindered by the lack of a robust and convenient animal model. we propose the infection of athymic nude rats with the rat hev strain la-b350 as suc ... | 2016 | 27483350 |
| hepatitis e virus antibody prevalence, rna frequency, and genotype among blood donors in cambodia (southeast asia). | recent studies conducted in developed countries described hepatitis e virus (hev) as an emerging infectious threat to blood safety. however, data on hev among blood donors from southeast asia are lacking. | 2016 | 27480100 |
| susceptibility of pigs to zoonotic hepatitis e virus genotype 3 isolated from a wild boar. | in europe, zoonotic hepatitis e virus (hev) genotype 3 strains mainly circulate in humans, swine and wild boar. the aim of this study was to investigate the potential transmission of a wild boar originating hev strain (wbhev) to swine by intravenous or oral inoculation and to study the consequences of infection of a wbhev strain, a wbhev strain previously passaged in a pig and a swine hev strain after oral inoculation. firstly, an intravenous infection was performed for which five piglets were d ... | 2016 | 27477944 |
| evolution of hepatitis e virus-associated meningo-polyradiculoneuropathy on ribavirin. | 2016 | 27470489 | |
| increased hepatitis e virus seroprevalence correlates with lower cd4+ cell counts in hiv-infected persons in argentina. | hepatitis e virus (hev) is a single-stranded rna virus that can cause hepatitis in an epidemic fashion. hev usually causes asymptomatic or limited acute infections in immunocompetent individuals, whereas in immunosuppressed individuals such as transplant recipients, hev can cause chronic infections. the risks and outcomes of hev co-infection in patients infected with human immunodeficiency virus (hiv) are poorly characterized. we used a third generation immunoassay to measure serum igg antibodie ... | 2016 | 27467394 |
| crystal structure of the human astrovirus capsid protein. | human astrovirus (hastv) is a leading cause of viral diarrhea in infants and young children worldwide. hastv is a nonenveloped virus with a t=3 capsid and a positive-sense rna genome. the capsid protein (cp) of hastv is synthesized as a 90-kda precursor (vp90) that can be divided into three linear domains: a conserved n-terminal domain, a hypervariable domain, and an acidic c-terminal domain. maturation of hastv requires proteolytic processing of the astrovirus cp both inside and outside the hos ... | 2016 | 27466429 |
| hepatitis e: prevention and treatment. | infection with the hepatitis e virus (hev) is very common worldwide. the epidemiology, viral genotypes, and transmission routes differ between low-resource countries and economically developed countries. these differences have resulted in the design of diverse prevention and treatment strategies to combat hev. | 2016 | 27454402 |
| hepatitis e viral loads in plasma pools for fractionation. | it is now recognized that blood donors may be silently infected with hepatitis e virus (hev) and that plasma pools used in the manufacture of plasma-derived medicinal products may also contain detectable virus rna. the occurrence of hev-infected blood and plasma donors can vary considerably depending on local epidemiology. | 2016 | 27453197 |
| production of infectious dromedary camel hepatitis e virus by a reverse genetic system: potential for zoonotic infection. | the pathogenicity, epidemiology and replication mechanism of dromedary camel hepatitis e virus (dchev), a novel hepatitis e virus (hev), has been unclear. here we used a reverse genetic system to produce dchev and examined the possibility of zoonotic infection. | 2016 | 27449916 |
| viral macro domains reverse protein adp-ribosylation. | adp-ribosylation is a posttranslational protein modification in which adp-ribose is transferred from nad(+) to specific acceptors to regulate a wide variety of cellular processes. the macro domain is an ancient and highly evolutionarily conserved protein domain widely distributed throughout all kingdoms of life, including viruses. the human targ1/c6orf130, macrod1, and macrod2 proteins can reverse adp-ribosylation by acting on adp-ribosylated substrates through the hydrolytic activity of their m ... | 2016 | 27440879 |
| islet cell transplantation from göttingen minipigs to cynomolgus monkeys: analysis of virus safety. | xenotransplantation using pig cells, tissues or organs may be associated with the transmission of porcine zoonotic micro-organisms. hepatitis e virus (hev), porcine cytomegalovirus (pcmv) and porcine endogenous retroviruses (pervs) are potentially zoonotic micro-organisms which do not show clinical symptoms in pigs and which are due to the low expression level difficult to detect. göttingen minipigs (gömp) are often used for biomedical investigations and they are well characterized concerning th ... | 2016 | 27440468 |
| possible foodborne transmission of hepatitis e virus from domestic pigs and wild boars from corsica. | 2016 | 27437570 | |
| genotype 1 hepatitis e virus infection with acute acalculous cholecystitis as an extrahepatic symptom: a case report. | hepatitis e virus (hev) causes an acute viral hepatitis that is transmitted enterically. it is epidemic in africa, asia, the middle east, and central america. it is known that hev can cause extrahepatic manifestations. here, we report the first case of acalculous cholecystitis as an extrahepatic symptom of hev. | 2016 | 27433137 |
| hepatitis e virus: assessment of the epidemiological situation in humans in europe, 2014/15. | hepatitis e virus (hev) is endemic in eu/eea countries, but the understanding of the burden of the infection in humans is inconsistent as the disease is not under eu surveillance but subject to national policies. | 2016 | 27393938 |
| detection and genetic characterization of hepatitis e virus (hev) genotype 3 subtype c in wild boars in italy. | hepatitis e virus (hev) was detected in stools collected from wild boars in italy, with an overall prevalence of 1.5 % (3/196). the sequence of a ~3.0-kb portion at the 3' end of the genome of one such strain, hev/wb/p6-15/ita, was determined. in the full-length orf2, which encodes the capsid protein, the virus was genetically closest to wild boar and human hev strains currently classified as genotype 3 subtype c. interestingly, the 3' end of orf2 of the wb/p6-15/ita matched the 340-nucleotide ( ... | 2016 | 27393602 |
| guillain-barré and miller fisher syndromes in patients with anti-hepatitis e virus antibody: a hospital-based survey in japan. | there have been reports from several countries that hepatitis e virus (hev) infection is frequently associated with guillain-barré syndrome (gbs). this study aimed to determine the frequency of hev infection associated with gbs in japanese patients, and to clarify the clinical characteristics of these patients. sera obtained from 63 patients with gbs or miller fisher syndrome (mfs) and 60 control subjects were examined for both hev-igm and hev-igg. of the 63 patients, three were positive for bot ... | 2016 | 27389141 |
| low transfusion transmission of hepatitis e among 25,637 single-donation, nucleic acid-tested blood donors. | hepatitis e virus genotype-3 (hev-gt-3) causes autochthonous infections in western countries, with a primary reservoir in animals, especially pigs. hev transfusion transmission has been reported, and hev-gt-3 prevalence is high in some european countries. the prevalence of hev rna was investigated among danish blood donors, and the prevalence of hev transfusion-transmitted infection (tti) was investigated among recipients. | 2016 | 27385646 |
| hepatitis e virus: a cross-sectional serological and virological study in pigs and humans at zoonotic risk within a high-density pig farming area. | an increase in autochthonous hepatitis e virus (hev) infections has been recorded in italy suspected to be zoonotically transmitted from pigs; this study was carried out to determinate the seroprevalence and risk factors associated with hepatitis hev exposition, both in swine and humans working in pig farms, located within a high-density pig farming area in piedmont region, north-western italy. the presence of viral rna in human and swine samples was also evaluated, and phylogenetic analysis was ... | 2016 | 27380833 |
| [neurological symptoms with a hepatitis e virus infection]. | infection with hepatitis e virus genotype 3 (hev3) is an emerging zoonosis in the industrialized world. the infection usually proceeds asymptomatically. extrahepatic sequelae including neurological symptoms have been described. | 2016 | 27378260 |
| the relationship between serology of hepatitis e virus with liver and kidney function in kidney transplant patients. | although hepatitis e virus (hev) is well known to cause acute hepatitis, there are reports showing that hev may also be responsible for progression of acute to chronic hepatitis and liver cirrhosis in patients receiving organ transplantation. in this study, we aimed to evaluate the prevalence of hev in patients with kidney transplantation. in this study, 110 patients with kidney transplantation were recruited, and anti-hev igg, creatinine, alanine transaminase (alt), aspartate aminotransferase ( ... | 2016 | 27366144 |
| hepatitis e in israel: a nation-wide retrospective study. | to investigate the epidemiology, risk factors and clinical course of acute hepatitis e virus (hev) infection in israel, an industrialized country. | 2016 | 27350735 |
| corrigendum to "hepatitis e virus infection in the hiv-positive patient" [j. clin. virol. 80 (2016) 102-106]. | 2016 | 27345249 | |
| hepatitis e virus infection--a new threat for europe. | of 20 million of patients infected with hepatitis e virus (hev) worldwide 57 thousand dies each year. hev-infection is not longer regarded as a diseases in developing endemic countries of asia, africa and latin america. the majority of european countries faces increasing number of endemic infections. they are caused by seven different genotypes and be responsible for acute and chronic infections. hev is of zoonotic origin causing infections in pigs and boars which are a source of infection for h ... | 2016 | 27344467 |
| clinical and virological profiling of sporadic hepatitis e virus infection in china. | recently, genotype 4 hev (hev-4) associated hepatitis e has overtaken hev-1 infections in china, but few studies reported the clinical and virological features of hev-4 infection. | 2016 | 27343562 |
| ifn regulatory factor 1 restricts hepatitis e virus replication by activating stat1 to induce antiviral ifn-stimulated genes. | ifn regulatory factor 1 (irf1) is one of the most important ifn-stimulated genes (isgs) in cellular antiviral immunity. although hepatitis e virus (hev) is a leading cause of acute hepatitis worldwide, how isgs counteract hev infection is largely unknown. this study was conducted to investigate the effect of irf1 on hev replication. multiple cell lines were used in 2 models that harbor hev. in different hev cell culture systems, irf1 effectively inhibited hev replication. irf1 did not trigger if ... | 2016 | 27328944 |
| hepatitis e virus rna-dependent rna polymerase: rna template specificities, recruitment and synthesis. | hepatitis e virus (hev) is a positive-sense rna virus and member of the genus orthohepevirus in the family hepeviridae. although hev rna-dependent rna polymerase (hev-rdrp) plays an important role in the hev life cycle, its template specificities are not completely understood. we expressed hev-rdrp protein with his-tag in a bacterial system and analysed template specificities using different putative cis-regulatory elements in the hev genome. the enzyme showed highest affinity for the 3' non-cod ... | 2016 | 27324050 |
| clinical and virological features of acute hepatitis e in gunma prefecture, japan between 2004 and 2015. | to evaluate the clinical and virological features of acute hepatitis e (ah-e) in gunma prefecture and focus on the hepatitis e virus (hev) infection in immunocompromised patients. | 2016 | 27322051 |
| detection of subtle differences in analogous viral capsid proteins by allowing unrestricted specific interaction in solution competition elisa. | assay artifacts were reported in plate-based immuoassays during the assessment of specific molecular interactions owing to the surface induced aggregation/conformational changes. to circumvent surface adsorption and associated artifacts, we used a solution competition elisa by allowing unrestricted interaction between binding partners to occur in solution for better discrimination between epitopes with subtle differences. a difference of two orders of magnitude in binding to neutralizing antibod ... | 2016 | 27321427 |
| report of a novel c1483w mutation in the hepatitis e virus polymerase in patients with acute liver failure. | here, we report the molecular alterations in the hev genome from patients with acute liver failure (alf) and acute viral hepatitis (avh) from north india, including pregnant women and its association with the poor outcome of the disease. we partially sequenced the rna dependent rna polymerase (rdrp) region of the orf 1 protein in the hev genome from representative samples from patients with alf and avh and identified two novel mutations cysteine 1483 tryptophan and asparagine 1530 threonine in 1 ... | 2016 | 27320795 |
| hepatitis e virus infection in dromedaries, north and east africa, united arab emirates, and pakistan, 1983-2015. | a new hepatitis e virus (hev-7) was recently found in dromedaries and 1 human from the united arab emirates. we screened 2,438 dromedary samples from pakistan, the united arab emirates, and 4 african countries. hev-7 is long established, diversified and geographically widespread. dromedaries may constitute a neglected source of zoonotic hev infections. | 2016 | 27315454 |
| acute graft dysfunction 17 years after liver transplant: a challenging clinical and histologic manifestation of hepatitis e. | acute hepatitis e virus infection after liver transplant is a challenging clinical phenomenon. due to its unspecific clinical and histological presentation, the diagnosis of acute or chronic hepatitis e virus infection can be difficult in unclear cases of elevated liver enzymes. here, we report the case of a 56-year-old male patient who presented to our center for 17-year follow-up after liver transplant with α1-antitrypsin deficiency. the patient was asymptomatic but had remarkably increased tr ... | 2016 | 27310664 |
| epidemiology of hepatitis e virus in iran. | iran is known as an endemic country for hepatitis e virus (hev) infection, while there are variations in the epidemiology of hev infection throughout the country. the available epidemiological studies in different regions of iran show hev seroprevalence of 1.1%-14.2% among general population, 4.5% -14.3% among blood donors, 6.1%-22.8% among injecting drug users, 6.3%-28.3% among hemodialysis patients, 1.6%-11.3% among patients infected with other hepatitis viruses, 27.5% among patients with chro ... | 2016 | 27298557 |
| excretion of infectious hepatitis e virus into milk in cows imposes high risks of zoonosis. | hepatitis e virus (hev) represents the main cause of acute hepatitis worldwide. hev infection in immunocompromised patients involves a high risk for the development of chronic hepatitis. because hev is recognized as a zoonotic pathogen, it is currently believed that swine is the primary reservoir. however, this is not sufficient to justify the strikingly high seroprevalence of hev in both developing and western countries. thus, this study aimed to identify new zoonotic sources that bear a high r ... | 2016 | 27286751 |
| differential immune responses in mice immunized with recombinant neutralizing epitope protein of hepatitis e virus formulated with liposome and alum adjuvants. | in the developing countries, hepatitis e virus (hev) is a predominant cause of sporadic acute hepatitis in adults and waterborne epidemics leading to high mortality in pregnant women. vaccine development mainly focuses on the structural capsid protein open-reading-frame-2 (orf-2) of the virus. we successfully evaluated liposome-adjuvanted recombinant neutralizing epitope protein (rnep), a part of orf-2, 458-607aa, in mice and rhesus macaques. we compared immune response to adjuvants alone, rnep ... | 2016 | 27285290 |
| divergent hepatitis e virus in birds of prey, common kestrel (falco tinnunculus) and red-footed falcon (f. vespertinus), hungary. | hepatitis e virus (hev), family hepeviridae, has raised considerable public health concerns because of its zoonotic potential; however, the animal to animal transmissions and the natural chain of hepevirus infections in wildlife are less known. using random amplification and next generation sequencing technology a novel hev in birds of prey was serendipitously identified in hungary. hev rna was detected in total of 2 (18%) of the 11 and 1 (14%) of the 7 faecal samples from common kestrels and re ... | 2016 | 27282471 |
| epidemiological characteristics and clinical manifestations of hepatitis e virus infection in bulgaria: a report on 20 patients. | hepatitis e is one of the leading clinical manifestations of acute viral hepatitis in developing countries. in industrialized countries, during the past several years, sporadic "autochthonous" cases of hev infection have been increased. | 2016 | 27276860 |
| the orf3 protein of genotype 1 hepatitis e virus suppresses tlr3-induced nf-κb signaling via tradd and rip1. | hepatitis e virus (hev) genotype 1 infection is common and can emerge as outbreaks in developing areas, thus posing a threat to public health. however, due to the absence of feasible animal models, the mechanism of he pathogenesis remains obscure. the hev pathogenic mechanism has been suggested to be mediated by the immune system and not by direct viral duplication. we firstly discovered that the open reading frame 3 (orf3) protein of genotype 1 hev downregulates tlr3-mediated nf-κb signaling in ... | 2016 | 27270888 |
| [infection by the hepatitis e virus as a precipitating factor of parsonage-turner syndrome]. | 2016 | 27270680 | |
| hepatitis e seroprevalence and related risk factors among seafood processing workers: a cross-sectional survey in shandong province, china. | this study aimed to assess the seroprevalence of hepatitis e virus (hev) infection and the potential risk factors for acquiring hev infection in the seafood processing factories in yantai city of shandong province, china. | 2016 | 27265612 |
| new insights into the hepatitis e virus genotype 3 phylodynamics and evolutionary history. | hepatitis e virus (hev) is an emergent hepatotropic virus endemic mainly in asia and other developing areas. however, in the last decade it has been increasingly reported in high-income countries. human infecting hev strains are currently classified into four genotypes (1-4). genotype 3 (hev-3) is the prevalent virus genotype and the mostly associated with autochthonous and sporadic cases of hev in developed areas. the evolutionary history of hev worldwide remains largely unknown. in this study ... | 2016 | 27264728 |
| [hepatitidis e virus]. | the number of detected cases of hepatitis e (he) shows an increasing trend in the czech republic, probably due the introduction of new diagnostic methods and greater awareness of he. in most cases, the source of infection has remained unconfirmed or entirely unclear. the causative agent, the hepatitis e virus (hev), belongs to the hepeviridae family. recently, many isolates from mammals, birds, and fish have been identified. hev is widespread among farm pigs and wild boar populations all over th ... | 2016 | 27246639 |
| hepatitis e virus genotype 3 in sewage and genotype 1 in acute hepatitis cases, israel. | hepatitis e virus (hev) is an emerging infectious agent in developed countries. hev genotypes 1 (g1) and 3 (g3) have been identified in environmental and clinical samples in europe. in israel, the overall prevalence of anti-hev igg antibodies was found to be 10.6%; however, reports of hev infection are scarce. in this study, the presence of hev in israel was investigated using 169 sewage samples from 32 treatment facilities and 49 samples from acute hepatitis patients, all collected between 2013 ... | 2016 | 27246446 |
| hepatitis e virus infection in the hiv-positive patient. | hepatitis e virus (hev) is a rna virus that can cause hepatitis. in immunocompetent individuals, infection with hev usually leads to asymptomatic seroconversion. however, in immunosuppressed patients, such as transplant recipients, hev can develop into a chronic infection. studies regarding the seroprevalence and clinical implications of hev in patients infected with the human immunodeficiency virus (hiv) are conflicting. levels of cd4 count in blood seem to be the most widely associated risk fa ... | 2016 | 27243210 |
| e hepatitis seroprevalence in a population of patients with transaminases increased at le mans' hospital (france). | in europe, infection through the hepatitis e virus presents itself under the form of autochthone, sporadic cases with zoonotic transmission. the diagnosis is more frequent in the case of male subjects above 50, with a generally severe clinical presentation. moreover, a transition to chronicity is described in the case of immunocompromised patients. in france and in the world, seroprevalence has not clearly been established yet. in the hospital of le mans, sarthe, france, the serologic status reg ... | 2016 | 27237808 |
| hepatitis e virus (hev) orf2 antigen levels differentiate between acute and chronic hev infection. | hepatitis e virus (hev) genotype 3 infections are frequent in europe and north america, with acute and chronic courses described in the literature. hev rna detection by real-time polymerase chain reaction (pcr) is the gold standard for diagnosis. recently, an anti-hev antigen (ag)-specific enzyme-linked immunosorbent assay (elisa) directed against the hev capsid became commercially available. the effectiveness of anti-hev ag-specific elisa at detecting hev genotype 3 infections remains undefined ... | 2016 | 27234418 |