Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| invasive amoebiasis is associated with the development of anti-neutrophil cytoplasmic antibody. | features of tissue damage in invasive amoebiasis, in particular polymorphonuclear neutrophil (pmn) degranulation and vasculitis, bear resemblance to that seen in wegener's granulomatosis, the latter being associated with the presence of anti-neutrophil cytoplasmic antibodies (anca). we therefore tested sera from patients with confirmed amoebic liver abscess (ala) for the presence of anca by means of an indirect fluorescent antibody test using pure neutrophils as substrate. anca was detected in 9 ... | 1994 | 8033420 |
| entamoeba histolytica and e. dispar. | 1994 | 8036693 | |
| adherence and cytotoxicity of entamoeba histolytica or how lectins let parasites stick around. | 1994 | 8039871 | |
| cloning of entamoeba genes encoding proteolipids of putative vacuolar proton-translocating atpases. | molecular cloning techniques were used to identify genes encoding the proteolipids of putative vacuolar proton-transporting atpases (v-atpases; ec 3.6.1.35) of entamoeba histolytica (ehvma3) and entamoeba dispar. the ehvma3 gene encoded a 177-amino-acid peptide, with an m(r) of 18,110, which showed extensive positional identities with peptides of e. dispar (92%), schizosaccharomyces pombe (58%), and humans (56%). | 1994 | 8039932 |
| transfection and transient expression of chloramphenicol acetyltransferase gene in the protozoan parasite entamoeba histolytica. | hybrid plasmids were constructed and used for successful transfection and transient expression of the chloramphenicol acetyltransferase (cat) gene in the protozoan parasite entamoeba histolytica. transfection was performed by electroporation of the amebae in a potassium phosphate-based buffer under conditions of 3000 v/cm and 25 microf, resulting in a time constant of 0.4 ms. expression of cat activity was achieved with constructs in which the cat coding region was flanked by untranslated upstre ... | 1994 | 8041751 |
| transient transfection of the enteric parasite entamoeba histolytica and expression of firefly luciferase. | development of dna-mediated transfection in entamoeba histolytica will facilitate basic research toward the control of this protozoan parasite. a transient transfection system was established by using the firefly luciferase gene ligated to the 5' and 3' flanking regions of the amebic hgl1 gene. the optimal construct tested encoded an hgl1-luciferase fusion protein and contained 1 kb of 5' flanking sequence with 16 bases of coding sequence from the hgl1 gene ligated in-frame to the luciferase sta ... | 1994 | 8041752 |
| [epidemiologic setting of the agricultural use of sewage: valle del mezquital, mexico]. | wastewater from mexico city is used to irrigate over 85,000 hectares mainly of fodder and cereal crops in the mezquital valley. a cross-sectional study method is being used to test the impact of exposure to raw wastewater and wastewater from storage reservoirs on diarrheal disease and parasitic infections in farmworkers and their families. the study population in the rainy season survey included 1,900 households: 680 households where the farmworker is exposed to untreated wastewater (exposed gro ... | 1994 | 8042068 |
| characterization of monoclonal antibodies to conserved antigens of entamoeba histolytica and e. dispar and development of a stool elisa. | eight murine monoclonal antibodies (mabs) were generated against entamoeba histolytica nih:200. all mabs reacted with three axenic strains of e. histolytica, nih:200, hm-1: imms, and saw 1734r clar, but not with enteric bacteria or giardia lamblia. five of the mabs reacted with low-molecular-weight, periodate-sensitive antigens of 14-21 kd, while one (ab31) reacted with high-molecular-weight, 90 to 200-kd protein determinants. mab pc14 appeared to be specific for antigen in its native state. ano ... | 1994 | 8050777 |
| exercise-induced diarrhea: when to wonder. | exercise-associated lower gastrointestinal symptoms seem to be a fairly common clinical finding occurring in anywhere from 10-50% of runners. however, it is unclear what percentage of those affected may have discrete medical conditions that are exacerbated by strenuous physical exertion. perhaps, difficult physical training sessions function as a "stress test" for the colon. a case report of exercise induced diarrhea is presented with a discussion of the current medical literature and proposed m ... | 1994 | 8007797 |
| [an analysis of the antigenic structure of the membrane complex in leishmania]. | 1994 | 8015520 | |
| symptomatic amebic colitis in a japanese homosexual aids patient. | a 30-year-old japanese homosexual aids patient was admitted to hospital because of kaposi's sarcoma and mild diarrhea on february 4, 1993. mud-like stool with blood and mucous was recognized after admission. although serologic tests for entamoeba histolytica were negative, trophozoites of e. histolytica were identified in his bloody stool. daily doses of 1,000 mg of metronidazole were given orally for 6 days and then 750 mg for 4 days, and good results were observed. his cd4 count was 19.5/mm3. ... | 1994 | 8019042 |
| isolation and characterization of a species-specific multicopy dna sequence from entamoeba histolytica. | a genomic library of entamoeba histolytica (pathogenic strain hm-1:imss) was screened to detect repetitive dna clones other than those from the highly abundant ribosomal dna (rdna). one such clone (hmc) had a 2.3 kb insert which hybridized with the main genome and not the rdna circle. southern hybridization of e. histolytica genomic dna, digested with ecor i and probed with hmc, showed multiple bands. the banding pattern was identical in all axenic pathogenic strains tested. differences, however ... | 1994 | 8022651 |
| intestinal parasitism in the united states: update on a continuing problem. | to document patterns of intestinal parasitism in the united states, we analyzed results of 216,275 stool specimens examined by the state diagnostic laboratories in 1987; parasites were found in 20.0%. percentages were highest for protozoans: giardia lamblia (7.2%), entamoeba coli and endolimax nana (4.2% each), blastocystis hominis (2.6%), and entamoeba histolytica (0.9%). the most commonly identified helminths were nematodes: hookworm (1.5%), trichuris trichiura (1.2%), and ascaris lumbricoides ... | 1994 | 8024063 |
| antiparasitic activity of diallyl trisulfide (dasuansu) on human and animal pathogenic protozoa (trypanosoma sp., entamoeba histolytica and giardia lamblia) in vitro. | garlic (allium sativum l.) and one of its major components, allicin, have been known to have antibacterial and antifungal activity for a long time. diallyl trisulfide is a chemically stable final transformation product of allicin which was synthesized in 1981 in china and used for treatment of bacterial, fungal and parasitic infections in man. the activity of diallyl trisulfide was investigated in several important protozoan parasites in vitro. the ic50 (concentration which inhibits metabolism o ... | 1994 | 8024350 |
| diagnosis of amebic dysentery by detection of entamoeba histolytica fecal antigen by an invasive strain-specific, monoclonal antibody-based enzyme-linked immunosorbent assay. | an invasive strain-specific monoclonal antibody against entamoeba histolytica has been used in a capture enzyme-linked immunosorbent assay (elisa) for the detection of invasive e. histolytica fecal antigen in clinical specimens and for the diagnosis of amebic dysentery in patients from bangladesh. the fecal antigen capture elisa (fac-elisa) did not cross-react with other parasite species in the clinical specimens or with noninvasive e. histolytica present in those specimens and in experimentally ... | 1994 | 8027351 |
| amebic abscess of the spleen complicated by metronidazole-induced neurotoxicity: case report. | we describe a patient with a liver abscess due to entamoeba histolytica, in whom metronidazole therapy (total dose, 21 g over 14 days) was complicated by reversible deafness, tinnitus, and ataxia and who relapsed 5 months later with a splenic abscess. two courses of tinidazole (the second in combination with percutaneous aspiration of the abscess) were required to achieve cure. | 1994 | 7986915 |
| prevalence of intestinal parasites in rural areas of district shahjahanpur, uttar pradesh. | a stool survey was carried out in some of the villages of dadraul and bhawal khera phc's of district shahjahanpur (uttar pradesh). out of 381 individuals examined 111 (29.2 per cent) were found positive for one or the other intestinal parasite. ascaris lumbricoides superseded all the parasites by showing a positivity of 17.85 percent. other parasites found were hookworm, hymenolepis nana, tapeworm, trichuris trichiura, enterobius vermicularis, entamoeba histolytica, e. coli and giardia lamblia. ... | 1994 | 7989672 |
| detection of immune complexes in tissues of persons infected with entamoeba histolytica. | immune complexes were detected by radioimmunoassay in colonic tissue from 71 to 122 patients (58%) excreting entamoeba histolytica cysts and in liver tissue from 5 of 16 of these patients. complexes were also found in colonic tissue from 9 of 56 other patients (16%) who were not excreting cysts. | 1994 | 7992334 |
| a beta 1 integrin-like molecule in entamoeba histolytica. | human invasive amoebiasis is highly destructive, causing rapid necrosis and liquefaction of all tissues reached by the trophozoites. degradation of extracellular matrix components (emc) has been demonstrated during invasion of the basal lamina. pursuing the idea that trophozoites might behave similarly to other invasive cells with respect to their interaction with emc, plasma membrane proteins biochemically or functionally related to integrins were looked for. a 140 kda molecular mass membrane p ... | 1994 | 7992351 |
| purification and immunologic characterization of a 30-kda cysteine proteinase of entamoeba histolytica. | a 30-kda cysteine proteinase was purified from extracts of axenically grown trophozoites of entamoeba histolytica strain hm1:imss. the purification procedure involved two consecutive chromatographic steps. sequence analysis revealed high similarity with histolysin and with other 27-kda cysteine proteinase. western-blot analysis using f(ab')2 fragments of a polyclonal antibody raised against the purified enzyme revealed that when the amebic extract was prepared in the absence of proteinase inhibi ... | 1994 | 7855127 |
| encysted ascites caused by entamoeba histolytica. | 1994 | 7860489 | |
| serodiagnostic evaluation of amoebic hepatitis. | 1994 | 7860573 | |
| effect of histamine and glucose on encystation of entamoeba histolytica in vitro. | isolates of entamoeba histolytica grown and maintained in modified boeck and drbohlav's medium were used to examine the effect of histamine and glucose on encystation process in vitro. this revealed that 60-74% of the active and motile trophozoites of e. histolytica encysted within 72 hours in the presence of histamine and glucose, in contrast to 10-20% encystment in controls (with out histamine and glucose); a highly significant difference (p < 0.001). these observations also suggest the possib ... | 1994 | 7863552 |
| [entamoeba histolytica (schaudinn 1903) and entamoeba dispar (e. brumpt 1925) are 2 different species]. | on the basis of epidemiologic and clinical data in humans, and of experimental studies in kittens, e. brumpt in 1925 showed that entamoeba histolytica producing quadrinucleate cysts was a complex of 2 species. he differentiated a pathogenic and invasive form, from a non pathogenic and non invasive one that he called entamoeba dispar. his explanation gained little support until recently, when sargeaunt reported a study of isoenzyme typing and allow then the classification of amoebae resembling e. ... | 1994 | 7866041 |
| [amoebiasis--a problem in patients with ulcerative colitis]. | the increasing problem is presented of entamoeba histolytica invasion in patients with ulcerative colitis. the diagnostic-therapeutic management in five patients with amoebiasis is discussed in detail. this parasitic invasion occurred in five out of 103 patients with ulcerative colitis which accounts for 4.85%. this data is important for the treatment of chronically ill patients with ulcerative colitis. | 1994 | 7941573 |
| the sensitivity of axenic 200:nih entamoeba histolytica to bacitracin and its zinc salt. | this study extends the previous knowledge of the action of bacitracin zinc against xenic cultures of entamoeba histolytica. results presented here, using axenic e. histolytica, confirm the enhanced activity of bacitracin zinc as compared to native bacitracin and demonstrate that this enhancement is due to a direct effect upon the trophozoite. in the presence of zinc, amoebastatic concentrations of bacitracin became amoebacidal. the expression of the amoebacidal characteristics of 5 g/l bacitraci ... | 1994 | 7942350 |
| secretory immunoglobulin a antibodies to the galactose-inhibitable adherence protein in the saliva of patients with amebic liver disease. | monoclonal antibodies directed against the 260-kd galactose-inhibitable adherence protein (giap) of entamoeba histolytica inhibit binding of amebic trophozoites to purified colonic mucins, suggesting that anti-giap secretory immunoglobulin a (siga) may have a role in host defense in invasive amebiasis. we determined by enzyme-linked immunosorbent assay (elisa) whether a salivary anti-giap siga response was present in patients from the republic of south africa with invasive e. histolytica infecti ... | 1994 | 7943572 |
| cyclospora infection in adults infected with hiv. clinical manifestations, treatment, and prophylaxis. | to determine the prevalence and clinical manifestations of cyclospora in haitians infected with human immunodeficiency virus (hiv) who have diarrhea and to evaluate therapy and prophylaxis. | 1994 | 7944073 |
| antibacterial activity of metal complexes of benzil and benzoin thiosemicarbazones. | the metal (ii) complexes of benzyl and benzoin thiosemicarbazones have been synthesized and characterized. they were studied for their antibacterial, antifungal and amoebicidal activity in vitro and for their toxicity in vivo. the ligands and their metal complexes exhibited low antibacterial and amoebicidal activity. the metal chelates of benzoin thiosemicarbazone seem to be the most active compounds. | 1994 | 7945719 |
| amebiasis: human infection with entamoeba histolytica. | 1994 | 7948936 | |
| [effects of monoclonal antibodies on phagocytosis of entamoeba histolytica to human erythrocytes]. | two monoclonal antibodies 3f7 and 4g6 were produced respectively against plasma membrane and nucleus-cytoplasm of entamoeba histolytica. the erythrocytophagous quantification and ability of pathogenic strain hm-1:imss were evaluated by microscopy immediately at 5 min and 10 min after incubation with mcab 3f7 and mcab 4g6. sera from normal mice and monoclonal antibody tce 04 against trypanosoma cruzi were prepared as negative controls. the mcab 3f7 showed stronger inhibitory action on the erythro ... | 1994 | 7955164 |
| entamoeba histolytica: mechanism of surface receptor capping. | binding of antibodies to the surface of the parasitic protozoan entamoeba histolytica induces capping and release of antigen-antibody complexes, in a process that may be an effective means of evading the host immune response. to analyze the mechanism of capping in e. histolytica trophozoites, optimal conditions were determined for cap induction by sera from patients with amebic liver abscess, by polyclonal anti-amebic antibodies, and by concanavalin a. cocapping of actin and myosin was documente ... | 1994 | 7957761 |
| entamoeba histolytica: pkc transduction pathway activation in the trophozoite-fibronectin interaction. | interaction of entamoeba histolytica trophozoites with fibronectin (fn) induces reorganization of the actin cytoskeleton and an increase in proteolytic activities that results in the degradation of the bound protein. the binding is mediated by a 37-kda fn "receptor" localized in the trophozoite surface and associated to the cytoskeleton. the intracellular signals triggered by the ligand-receptor interaction are not well understood but it is plausible that they drive the observed responses. to ad ... | 1994 | 7957762 |
| entamoeba histolytica: target cells killed by trophozoites undergo dna fragmentation which is not blocked by bcl-2. | amebic destruction of neutrophils and macrophages is contact-dependent. adherence is mediated by a galactose-specific surface lectin on the amebic membrane. the pathway by which contact-dependent cytolysis of the target cell occurs is unknown. we hypothesized that target cell death is due to the triggering of apoptosis (programmed cell death) by the amebae. the purpose of this study was to determine whether target cell dna is fragmented into a ladder pattern characteristic of apoptosis and to te ... | 1994 | 7957763 |
| characterization of two distinct gene transcripts for ribosomal protein l21 from pathogenic and nonpathogenic strains of entamoeba histolytica. | a second gene (rp-l21) copy, clone g34, coding for ribosomal (r-) protein l21, was isolated from the pathogenic (p) strain hm-1:imss cl6 of the intestinal parasite entamoeba histolytica (eh). the gene was compared to the previously isolated copy, gle3 [petter et al., mol. biochem. parasitol. 56 (1992) 329-334], with respect to its primary structure, mrna levels and binding to the r-complex during translation. unlike the gle3 gene copy [petter et al., mol. biochem. parasitol. 56 (1992) 329-334], ... | 1994 | 7959049 |
| [microbiological diagnosis of malaria and amoebiasis]. | all stages of malaria and amebiasis may be sensitively and specifically diagnosed by means of microbiological methods. microscopical parasitology holds a key position when supplemented by immunodiagnostic assays. molecular biology techniques are currently still inferior to the above procedure. tests for the differentiation between pathogenic and nonpathogenic entamoeba histolytica (entamoeba dispar) require further simplification before generally applicable. | 1994 | 7959857 |
| [therapy for malaria and amoebiasis]. | treatment of malaria depends on the infecting plasmodium species. in plasmodium falciparum malaria the treatment also depends on whether chloroquine resistances occur and whether the course is uncomplicated or complicated. uncomplicated cases are cared for with chloroquine and with mefloquine or halofantrine when the patient comes from areas with chloroquine resistances. patients with complicated plasmodium falciparum malaria must get chinine and doxycycline. a careful fluid balance is extremely ... | 1994 | 7959858 |
| detection of ubiquinone in parasitic and free-living protozoa, including species devoid of mitochondria. | ubiquinone (coenzyme q, coq) was analyzed and individual homologues quantified in 11 species of parasitic and free-living protozoa by a combination of thin-layer chromatography and high performance liquid chromatography. fast atom bombardment ionization-mass spectrometry was used for the first time to confirm the identity of the fractionated coq homologues and proved to be a fast, gentle and convenient method for ubiquinone identification. ubiquinone was detected in all organisms including those ... | 1994 | 7969263 |
| purification and molecular characterization of the nad(+)-dependent acetaldehyde/alcohol dehydrogenase from entamoeba histolytica. | a bifunctional 95 kda polypeptide (ehadh2) harbouring acetaldehyde dehydrogenase and alcohol dehydrogenase activities was purified to homogeneity from trophozoite extracts of the protozoan parasite entamoeba histolytica. kinetic studies revealed that the enzyme utilizes nad+ rather than nadp+ as cofactor. km values for acetyl-coa, acetaldehyde and ethanol were found to be 0.015, 0.15 and 80 mm respectively in the presence of 0.2 mm nad+. the primary structure of ehadh2 as deduced from respective ... | 1994 | 7980441 |
| [gnathostomiasis after a stay in thailand]. | during a 4-week holiday in thailand a 13-year-old boy from germany briefly fell ill with diarrhoea without blood-tinged stools and fever. 5 months later oedematous, reddish swellings occurred, at first on the right upper arm, later over the right side of the chest. especially at night these swellings were associated with severe local and generalized itching. bilaterally the axillary lymph-nodes were hard and slightly enlarged. there was a marked eosinophilia of 56%, the white count was 22,700/mi ... | 1994 | 7982372 |
| monoclonal antibody-based immunohistochemical demonstration of entamoeba histolytica in liver tissues of experimentally infected hamster (mesocricetus auratus). | histopathological changes and the presence of entamoeba histolytica trophozoites was sequentially followed after intrahepatic inoculation of the parasites in 42 hamsters, 35 of which received no treatment whereas the remaining seven were treated with metronidazole. the liver tissues were examined for amoebic trophozoites by a monoclonal antibody (mab)-based immunofluorescence assay (ifa), a mab-based immunoperoxidase (ipx) and h & e staining. the number of hamsters developing abscesses was incre ... | 1994 | 7982753 |
| expression and characterization of a rac family protein kinase of entamoeba histolytica. | we previously reported the isolation from entamoeba histolytica of a novel rac family protein kinase gene, termed ehrac1, for "related to camp-dependent protein kinases and protein kinase cs". to study the function and properties of this kinase gene further, we fused the full-length coding region and the truncated catalytic domain of the ehrac1 gene in frame with the gene encoding glutathione s-transferase in the pgex-kg vector and expressed the fusion in escherichia coli. the thrombin-cleaved a ... | 1994 | 7984173 |
| primary structure of the entamoeba histolytica gene (ehvma1) encoding the catalytic peptide of a putative vacuolar membrane proton-transporting atpase (v-atpase). | 1994 | 7984182 | |
| an intron-containing gene coding for a novel 39-kilodalton antigen of entamoeba histolytica. | 1994 | 7984185 | |
| [amebic liver abscess: review of 9 cases]. | amoebiasis is an infection of the large intestine produced by the protozoan entamoeba histolytica. more than 10% of the world population is estimated to be infected. the italian pattern of this infection is similar to the eec trend: the prevalence is approximately 0.012% during the years '88 and '89. hepatic abscesses usually present with moderate fever, weight loss and abdominal pain. during the last ten years nine patients with amoebic liver abscesses were admitted to our hospital, six males a ... | 1994 | 7761679 |
| standardization and evaluation of elisa for the serodiagnosis of amoebic liver abscess. | an elisa test for the serological diagnosis of amoebic liver abscess (ala) was standardized and evaluated in sera from three groups of patients: (1) three patients with diagnosis confirmed by isolation of the parasite, (2) thirty seven patients with diagnosis established by clinical findings and ultrasound studies and (3) seven patients whose diagnosis were established by clinical findings and a positive double immunodifusion test. ninety one serum samples from healthy subjects and 22 from patie ... | 1994 | 7823821 |
| cryptosporidiosis among medical patients with the acquired immunodeficiency syndrome in tikur anbessa teaching hospital, ethiopia. | fresh stool specimens, collected at random from 63 medical in-patients with acquired immunodeficiency syndrome (aids), were studied prospectively for cryptosporidium oocyst. the diagnosis of aids was made according to the clinical case definition of the bangui criteria. these patients presented with profuse watery diarrhoea, significant weight loss and other associated symptoms and signs of clinical manifestations of symptomatic human immunodeficiency virus (hiv) infection. using the modified ki ... | 1994 | 7835259 |
| enteropathogens associated with acute diarrhoeal diseases. | five types of escherichia coli are responsible for as much as 25% of all diarrheal diseases in developing countries. they tend to be transmitted via contaminated foods, particularly weaning foods, and water. they include enterotoxigenic, enteropathogenic, enteroadherent, enteroinvasive, and enterohemorrhagic e. coli. shigella species are responsible for 10-15% of acute diarrheas in children less than 5 years old and the most common etiologic agents of childhood dysentery. shigellosis is common i ... | 1994 | 7835992 |
| identification of a developmentally regulated transcript expressed during encystation of entamoeba invadens. | differentiation of trophozoites into cysts in entamoeba species has been described morphologically and to a lesser extent biochemically, but studies of stage specific gene expression have not been reported. at present entamoeba invadens is the only species that can be induced to differentiate in axenic culture and is a useful model system for the human parasite entamoeba histolytica. using this model system, we performed cdna-mrna hybridization experiments to compare the rna populations from tro ... | 1994 | 7838173 |
| intestinal parasitoses in the kandy area, sri lanka. | the prevalence of intestinal protozoa and geohelminths was assessed among two diverse populations in the kandy area: adults attending medical outpatients clinics at the teaching hospital peradeniya, and pre-school children in low-cost housing areas within the kandy municipality. in addition to a brief history and examination, a fresh stool sample was obtained and examined by direct smears in saline and iodine, and by formol-ether concentration. the children's stool samples were also examined for ... | 1994 | 7777909 |
| blastocystis hominis infection, a cause of human diarrhea. | blastocystis hominis has long been described as a non pathogenic protozoan parasite until recently when claims have been made that it can result in pathogenic conditions. of the 729 stool samples (614 from survey and 115 from pediatric wards) examined, 18.1% of them were found to be positive for one or more intestinal protozoan cyst. the commonest was giardia intestinalis (8.4%) followed by entamoeba coli (7.1%) and entamoeba histolytica (5.1%) in the normal children without symptoms of diarrhea ... | 1994 | 7777913 |
| [recent progress in the diagnosis of intestinal amebiasis. contribution of molecular biology]. | only 10% of entamoeba histolytica strains have been reported to have a pathogenic capacity. theoretically and under certain conditions, confirming this estimation should limit the number and the cost of the treatments administered to the four hundred million asymptomatic carriers in the world. several new routine tests which allow more accurate and easier diagnosis of intestinal amoebiasis and in particular the differential diagnosis between the pathogenic and nonpathogenic strains are or soon w ... | 1994 | 7802347 |
| intestinal amebiasis: histopathologic features in experimentally infected mice. | intestinal amebiasis is still an important health problem in developing regions of the world. in order to advance our knowledge on the pathogenesis and to test the relevance of recently obtained in vitro observations, suitable in vivo experimental models of intestinal amebiasis are needed. in the past a variety of laboratory animals have been used, but the mouse, whose genetics and immunology is well known, has been seldomly used. therefore, entamoeba histolytica strain hm1:imss was directly ino ... | 1994 | 7803977 |
| antiprotozoal activities of benzimidazoles and correlations with beta-tubulin sequence. | benzimidazoles have been widely used since the 1960s as anthelmintic agents in veterinary and human medicine and as antifungal agents in agriculture. more recently, selected benzimidazole derivatives were shown to be active in vitro against two protozoan parasites, trichomonas vaginalis and giardia lamblia, and clinical studies with aids patients have suggested that microsporidia are susceptible as well. here, we first present in vitro susceptibility data for t. vaginalis and g. lamblia using an ... | 1994 | 7811023 |
| nucleotide sequence organisation and analysis of the nuclear ribosomal dna circle of the protozoan parasite entamoeba histolytica. | we have sequenced the extrachromsomal ribosomal dna (rdna) circle of the human protozoan parasite entamoeba histolytica hm-1:imss and present here the complete sequence organisation of the 24.5-kb molecule. each circle contains two 5.9-kb rdna transcription units organised as inverted repeats. the regions downstream (3543 bp) and upstream (9216 bp) of the rdnas contain various families of short tandem repeats. some of the upstream repeats share extensive sequence homology with the downstream rep ... | 1994 | 7870125 |
| induction of the iron-containing superoxide dismutase in entamoeba histolytica by a superoxide anion-generating system or by iron chelation. | the regulation of superoxide dismutase (sod) expression was studied in 4 entamoeba histolytica isolates. in comparison to anaerobic conditions, cultivation of the amoebae in the presence of superoxide radical anions or a ferrous iron chelator revealed substantial increase of sod expression. under the different culture conditions, all sod activity could be exclusively attributed to an iron-containing type (fesod). northern blot analysis revealed that fesod expression was regulated on the transcri ... | 1994 | 7870132 |
| [necrotizing amebic colitis]. | we report a case of necrotizing amebic colitis in a 43 year-old patient, 10 years after a stay in senegal, successfully treated by right hemicolectomy. entamoeba histolytica were found in colon necrosis and amoebiasis serology was positive, leading to the diagnosis. the epidemiologic, physiopathologic, clinical and therapeutic features of this exceptional affection are described. a few cases have been previously reported in non endemic areas where pre-operative diagnosis is rarely made. in endem ... | 1994 | 7875425 |
| bile salts help entamoeba histolytica to evade from immune attack. | 1994 | 7896326 | |
| host-parasite relationships in amebiasis: conference report. | entamoeba histolytica has been redescribed as the separate species e. histolytica and entamoeba dispar. e. dispar is apparently never invasive in humans, while e. histolytica is the etiologic agent of amebic colitis and liver abscess. virulence factors that may enable e. histolytica to invade include a galactose-specific adhesin, secreted proteases, extracellular matrix receptors, and a cell surface lipophosphoglycan. progress in vaccine development includes the identification of the cysteine-ri ... | 1994 | 7908923 |
| brain abscess due to infection with entamoeba histolytica. | we successfully treated a serious case of entamoeba histolytica infection showing brain abscess, meningitis, and liver abscess by oral administration of metronidazole, intramuscular injection of dehydroemetine, and drainage of the brain abscess. the diagnosis of amebic brain abscess was based on a combination of clinical symptoms and signs, computed tomography and magnetic resonance imaging findings, a positive serologic test result for e. histolytica, a dramatic response to anti-amebic drugs af ... | 1994 | 7915500 |
| the use of an elispot assay to evaluate intestinal and systemic antibody responses to locally administered entamoeba histolytica antigen in mice. | the local induction of humoral immune response to entamoeba histolytica trophozoites in the gut has not been studied. this work reports some of our recent studies aimed to induce optimal immune responses against e. histolytica in mice and to describe a novel approach for monitoring mucosal immune responses induced in the gastrointestinal tract and expressed locally and systemically. we have compared the kinetics of both mucosal and systemic primary antibody responses to e. histolytica in the pey ... | 1994 | 7919810 |
| proteins of entamoeba histolytica trophozoites involved in the adhesion to target cells. | to identify the molecules involved in the adhesion of entamoeba histolytica trophozoites to target cells we used monoclonal antibodies (mabs) and adhesion-deficient mutants. human red blood cells (rbcs) were also used as specific carriers to identify the ameba molecules with affinity to the target cell receptors. mabs adh-1 and adh-2 inhibited adhesion of rbcs to the trophozoites and recognized a 112-kda surface protein that was present in the wild type strain, but was absent or modified in adhe ... | 1994 | 7919814 |
| a monoclonal antibody specific for a 40 kda polypeptide recognizes pathogenic strains of entamoeba histolytica. | in vitro interaction of entamoeba histolytica trophozoites with collagen induces the intracellular formation and release of electron-dense granules (edgs). we determined that four polypeptides in edgs total antigen by sds-page were absent in trophozoite extracts. a monoclonal antibody raised against these edgs recognized a polypeptide of 40 kda specific for the pathogenic strains of e. histolytica. in addition, this mab recognized a 96 kda peptide masked by carbohydrates. | 1994 | 7919818 |
| diagnosis of invasive amoebiasis--time to end the morphology era. | 1994 | 7926897 | |
| leucocyte migration inhibition index responses of patients of amoebic liver abscess to various antigens of entamoeba histolytica. | the whole amoebic lysate (wal), sephadex g-200 eluted fraction-i. (f-i), detergent dissected membrane proteins (ddmp), amoebic membrane glycoprotein (amg) and amoebal rna were extracted from e. histolytica. amoebal rna recognized 98.21% where as f-i, amg, ddmp and wal recognised 92.85, 91.07, 89.28 and 75% of total 56 clinically proven and amoebic serology positive amoebic liver abscess cases respectively. intensity of recognition of individual fraction i.e., leucocyte migration inhibition index ... | 1994 | 7927532 |
| entamoeba histolytica interactions with polarized human intestinal caco-2 epithelial cells. | to model the initial pathogenic effects of entamoeba histolytica trophozoites on intestinal epithelial cells, the interactions of e. histolytica hm1-imss trophozoites with polarized human intestinal caco-2 cell monolayers grown on permeabilized filters were examined. trophozoites, when incubated with the apical surface of the monolayers at 37 degrees c, induced a rapid decrease in transepithelial resistance over 15 to 60 min. the transmonolayer resistance response was not associated with changes ... | 1994 | 7927794 |
| heterogeneity of the ribosomal dna episome in strains and species of entamoeba. | ribosomal dna sequences in several species of the genus entamoeba are highly repeated and display restriction fragment-length polymorphism (rflp), which has been used to identify species and differentiate strains. however, the continuous variability of the non-transcribed repeat sequences in the ribosomal episome hinders an accurate typification. looking for more reliable markers, we used dna probes containing conserved sequences in the ribosomal episome--coding regions for the 16s and 5.8s rrna ... | 1994 | 7934884 |
| entamoeba histolytica has an alcohol dehydrogenase homologous to the multifunctional adhe gene product of escherichia coli. | entamoeba histolytica ferments glucose to ethanol under the anaerobic conditions of the human colon. there is special interest in this metabolic pathway because it provides an opportunity for parasite-specific chemotherapy. peptide sequences from a 97-kda e. histolytica protein, which was originally isolated because of extracellular matrix binding properties, were used to clone and sequence a gene that was found to encode an e. histolytica alcohol dehydrogenase and acetaldehyde dehydrogenase (eh ... | 1994 | 7935603 |
| myo-inositol trisphosphate-mediated calcium release from internal stores of entamoeba histolytica. | calcium mobilisation from internal stores of the parasitic protozoan entamoeba histolytica was studied by fluorescence measurements of the calcium indicator quin 2 and 45ca2+ incorporation studies in saponin-permeabilised amoebae. prior energy-dependent calcium sequestration was found to be necessary for subsequent release of calcium by inositol 1,4,5-trisphosphate (ins(1,4,5)p3). both ins(1,4,5)p3 and inositol 2,4,5-trisphosphate (ins(2,4,5)p3) could release calcium equally well from permeabili ... | 1994 | 7935629 |
| use of monoclonal anti-light subunit antibodies to study the structure and function of the entamoeba histolytica gal/galnac adherence lectin. | adherence of entamoeba histolytica trophozoites to host cells is mediated by a galactose (gal) and n-acetylgalactosamine (galnac)-specific surface lectin. the lectin is a heterodimeric protein composed of heavy (170 kda) and light (35-31 kda) subunits linked by disulfide bonds. polyclonal and monoclonal antibodies (mab) raised against a light subunit-glutathione-s-transferase fusion protein were used to probe its structure and function. four light subunit-specific mab were produced which recogni ... | 1994 | 7696848 |
| [epidemiology and etiology of infectious diarrheas. the case of mexico]. | gastrointestinal infections represent a health problem. it is estimated that 1647 million cases of diarrhea and 3.2 million deaths due to this cause occur among children less than five years of age per year. those belonging to this age group have 15 times more risk of dying because of diarrhea. cases of liquid acute diarrhea with blood represent 80% of cases, diarrhea with blood represent 10%. most frequent causes of liquid diarrhea are enterotoxigenic escherichia coli and rotaviruses and most f ... | 1994 | 7701141 |
| control of an amoebiasis outbreak in the philippi area near cape town. | previous studies in durban have shown that serological investigations, in combination with iso-enzyme electrophoresis, are invaluable for monitoring the endemicity of pathogenic strains of entamoeba histolytica. we therefore proposed that antibody profiles could be used to detect epidemic situations. an outbreak of amoebiasis in the normally non-endemic philippi area near cape town provided an opportunity for testing this hypothesis. seven of 9 patients presenting at a district hospital with inv ... | 1994 | 7709300 |
| [comparison of intestinal parasite infestation indexes among hiv positive and negative populations]. | this study was carried out on two groups of patients treated at the josé de san martín clinical hospital. one group was composed of 82 hiv-seropositive patients with no signs of diarrhea, and another one of 300 patients, not suspected of hiv infection, was considered as "control population". stool samples were collected from each patient and examined for intestinal parasites. these determinations were carried out at the department of clinical biochemistry, in the same hospital. three specimens w ... | 1994 | 7715428 |
| amoebapores, a family of membranolytic peptides from cytoplasmic granules of entamoeba histolytica: isolation, primary structure, and pore formation in bacterial cytoplasmic membranes. | three peptides with pore-forming activity were isolated from the cytoplasmic granules of pathogenic entamoeba histolytica by acidic extraction, gel filtration and reversed-phase high-performance liquid chromatography. partial amino acid sequence analysis of the three active peptides revealed that the most abundant of them was amoebapore and the other two were isoforms thereof. cloning and sequencing of genomic dna resolved the amino acid sequence of the two newly recognized peptides. the three p ... | 1994 | 7715451 |
| complement sensitivity of entamoeba histolytica and various nonpathogenic amoeba species. | culture forms of the potentially pathogenic entamoeba histolytica were compared to those of the nonpathogenic species of e. dispar, e. hartmanni, e. coli, endolimax nana, and e. moshkovskii regarding the sensitivity to lysis by human complement activated through the alternative pathway. e. dispar was found unique in its complement resistance; all other nonpathogenic isolates resembled e. histolytica in that they were complement sensitive. thus, a state of complement sensitivity is not a particul ... | 1994 | 7716404 |
| [a coprological study of intestinal infections in northern morocco (provinces of tangier, tetuán and larache)]. | an epidemiological study on intestinal parasites has been carried out in northern morocco (province of tangier, tetuan and larache). a comparative study has been made at a provincial, city and areas (urban and rural) level. also the spi (simple parasitism index) and the cpi (comparative parasitism index) distribution. | 1994 | 7716431 |
| [report on the first nationwide survey of the distribution of human parasites in china. 1. regional distribution of parasite species]. | a nationwide (taiwan province not included) survey of the distribution of human parasites in china during 1988-1992 was conducted under the auspices of the ministry of public health, with stratified masses randomly sampling. a total of 2,848 pilot sites in 726 counties with a population of 1,477,742 were surveyed, according to unified standard, unified diagnostic method and control quality. the overall infection rate of parasites was 62. 632%. among them, the infection rate was over 50% in 17 pr ... | 1994 | 7720194 |
| [effect of indirect fluorescent antibody test for the diagnosis of amoebiasis]. | indirect fluorescent antibody test (ifat) was performed by the method of [symbol: see text] (1971) with some slight modifications for the diagnosis of amoebiasis. the antigen used was prepared from 48-hour culture of entamoeba histolytica (strains g2, g3, and g5) in las diphasic medium and only those amoebae clinging the culture were collected. the serum samples or blood drops on filter paper were positive by the improved ifat in all of the 54 cases of amoebic liver abscess and the positive reac ... | 1994 | 7720200 |
| survey of the prevalence of intestinal parasites in children of primary school age. | a survey of the prevalence of intestinal parasites in primary school children was carried out in nimo in njikoka local government area of anambra state, nigeria. stool samples were taken from children belonging to eight (8) primary schools in the area. of the 1,536 stool samples examined using light microscopy after formalether centrifugation, 922 (60%) harboured one or more parasites. the prevalence of the parasites was as follows: ascaris lumbricoides (20.8%); trichuris trichiura, (15.3%); nec ... | 1994 | 7756189 |
| primary structure of an entamoeba histolytica nicotinamide nucleotide transhydrogenase. | 1994 | 7739679 | |
| [in vivo experimental models of amebiasis]. | in vivo experimental models for amebiasis have provided important information about the mechanisms of host-parasite interaction which determine the production of disease. in the laboratory, several species of rodents have been used to study the intestinal and hepatic amebiasis. for the former, the model of "washed-closed cecal loop" in guinea-pig and hamster has been useful to study the early invasive events. in gerbils we also produced early intestinal lesions by intracecal inoculation with mon ... | 1994 | 7557059 |
| [prevalence of infection by intestinal helminths and protozoa in school children from a coastal locality in the province of valdivia, chile]. | during july-august 1989, 219 coprological samples from primary school children from niebla and los molinos localities (39 degrees 52's, 73 degrees 26'w) in the coast of province of valdivia, chile, were analysed. prevalence % (in parentheses) of infection by intestinal protozoa and helminths were the followings: entamoeba histolytica: (18.0), entamoeba coli (34.0), endolimax nana (34.4), iodamoeba buetschlii (7.4), blastocystis hominis (64.3), giardia intestinalis (27.9) chilomastix mesnili (0.8 ... | 1994 | 7654292 |
| metastasis of entamoeba histolytica compared to colon cancer: one more step in invasion. | the multistep invasion process of metastasis is a well-conserved mechanism of normal symbiosis and pathogenic parasitosis. to illustrate this statement, we have compared cellular and molecular aspects of metastasis from colon cancer and enteric amebiasis caused by entamoeba histolytica. both types of pathogenic cell, cancer cells and amebic trophozoites, follow the same metastatic route for the liver and other organs. both establish invasion microecosystems implicating similar kinds of host cell ... | 1994 | 7657511 |
| human secretory immunoglobulin a anti-entamoeba histolytica antibodies inhibit adherence of amebae to mdck cells. | the presence of secretory immunoglobulin a (iga) anti-entamoeba histolytica antibodies in the saliva of patients with intestinal amebiasis was demonstrated by immunoblot assay, and the capacity of these antibodies to inhibit amebic adherence to a monolayer of mdck cells was analyzed. inhibition was due to iga antiamebic antibodies and in part to anti-gal-binding-lectin antibodies, as demonstrated by absorption experiments with total amebic extract and with the fraction of gal-binding lectin. the ... | 1994 | 8300242 |
| intestinal parasitic infections and swamp development in sierra leone. | the prevalence of entamoeba histolytica, giardia lamblia and other intestinal and urogenital parasites were assessed in five inland valley swamp (ivs) development faming communities in the moyamba district, south-central sierra leone. stool and urine samples were submitted by 1106 individuals and examined by the iron-haematoxylin staining and the formalin-ether concentration techniques for faecal sample and centrifugation method for the urine samples. the overall parasitic infection rate was 61. ... | 1994 | 12153344 |
| is phagocytosis related to virulence in entamoeba histolytica schaudinn, 1903? | phagocytosis has been suggested as a marker of pathogenicity and virulence in entamoeba histolytica. irmgard montfort and ruy pérez-tamayo here review the evidence for this suggestion. | 1994 | 15275444 |
| pathogenicity, virulence and entamoeba histolytica. | 1994 | 15275497 | |
| pore formation and cytolysis by entamoeba histolytica. | 1994 | 15275556 | |
| stool culture as a diagnostic aid in the detection of entamoeba histolytica in the faecal specimens. | "the use of stool culture in boeck & drbohlav's biphasic amoebic medium as a routine diagnostic aid in the detection of entamoeba histolytica in the faeces, is evaluated in the present study. a total of 3803 faecal specimens were examined for the presence of e.histolytica by direct smear, formalin ether concentration and culture during a study period of 1982-1990. a total of 259 stool specimens were positive for the parasite by any or all of these methods 42 (16.21%) stool specimen not cultured ... | 1995 | 9726144 |
| spontaneous release of cysteine proteinases but not of pore-forming peptides by viable entamoeba histolytica. | invasive properties of pathogenic entamoeba histolytica have been postulated to depend on the secretion or release of cysteine proteinases and pore-forming peptides (amoebapores) by trophozoites. to establish whether such toxic molecules are released by viable trophozoites or upon cellular disintegration, amoebae were maintained in various culture media, and activities in supernatants were monitored over time in correlation to cellular integrity. by measuring the release of the cytoplasmic marke ... | 1995 | 8559589 |
| identification of the galactose-adherence lectin epitopes of entamoeba histolytica that stimulate tumor necrosis factor-alpha production by macrophages. | the 170-kda subunit of the galactose-adherence lectin (gal-lectin) of entamoeba histolytica mediates adherence to human colonic mucins and intestinal epithelium as a prerequisite to amebic invasion. the gal-lectin is an immunodominant molecule and a protective antigen in the gerbil model of amebiasis. tumor necrosis factor alpha (tnf-alpha) produced by activated macrophages enhances nitric oxide-dependent cytotoxicity in host defense against e. histolytica. the purpose of this study was to ident ... | 1995 | 8618866 |
| evaluation of an enzyme-linked immunoassay for the detection in serum of antibodies to entamoeba histolytica. | a commercially available enzyme-linked immunoassay (elisa) was compared to an indirect hemagglutination test (iha) for the detection of antibodies to entamoeba histolytica on 225 patients' serums. all of the 107 serums that had iha titers of < 32 (interpreted as excluding the presence of invasive amebiasis) were elisa negative. sixty-three of 68 (93%) serums that had iha titers of > or = 128 (interpreted as indicative of the presence of active or recent infection) were elisa positive. fifty seru ... | 1995 | 8565416 |
| isolation and axenization of two entamoeba histolytica strains. | we describe the isolation and axenization of two e. histolytica strains, obtained from the stools of two patients with the clinical diagnosis of dysentery. we used pavlova's medium for initial polixenic culture, and tyi-s-33 (diamond's) medium for monoxenic and axenic cultures. in order to eliminate the microorganism contaminating the stools the following antibiotics were used: penicillin, streptomycin, ampicillin, chloramphenicol, kanamycin, nistatin, ceftriaxone and amphoterycin b. both strain ... | 1995 | 8566669 |
| rapid diagnosis of entamoeba infection by using entamoeba and entamoeba histolytica stool antigen detection kits. | humans are infected by two morphologically identical species of entamoeba: entamoeba histolytica causes amebic colitis and liver abscess, and entamoeba dispar is noninvasive. several weeks of culture and isoenzyme (zymodeme) analysis are required to differentiate e. histolytica from e. dispar. here we report a field trial of commercial antigen detection kits designed to rapidly detect and differentiate e. histolytica from e. dispar in stool specimens. stool specimens from 202 patients with diarr ... | 1995 | 8567882 |
| prevalence of enteric parasitic infection among hiv-infected attendees of an inner city aids clinic. | one hundred individuals confirmed to be infected with the human immunodeficiency virus (hiv), volunteered to participate in a pilot study comparing clinical status and enteric parasite burden with race, age, cd4 levels, risk factors and sexual practice. the prevalence of enteric parasites was 55 (55%) for giardia lamblia, 6 (6%) for cryptosporidium, 10 (10%) for isospora belli and 3 (3%) for entamoeba histolytica. there was no associations between demographic variables and the presence of parasi ... | 1995 | 8574144 |
| molecular analysis of two hexokinase isoenzymes from entamoeba histolytica. | the zymodemes, electrophoretic patterns of hexokinase, phosphoglucomutase and glucose phosphate isomerase isoenzymes, have been widely used to determine the pathogenicity of entamoeba histolytica isolates. although pathogenic and nonpathogenic forms of e. histolytica differ clearly in sequences of many homologous genes, a conversion between pathogenic and nonpathogenic zymodemes has been reported by several laboratories. to approach the question what might be the basis for the observed conversio ... | 1995 | 8577326 |
| identification and analysis of the start site of ribosomal rna transcription of entamoeba histolytica. | in this article we report the identification of the start site of ribosomal rna transcription unit of the enteric parasite e. histolytica. we cloned the upstream region of the ribosomal rna and we defined the 5' boundary of the transcription unit with nuclear run-on assays. we report that ribosomal transcription starts 2447 bp upstream the ssu ribosomal gene, at an adenosine residue. this data was supported both by s1 mapping and by primer extension analysis; that the mapped site was indeed the ... | 1995 | 8577327 |
| transcription of protein-coding genes in entamoeba histolytica is insensitive to high concentrations of alpha-amanitin. | 1995 | 8577336 | |
| kinetic mechanism of pyrophosphate-dependent phosphofructokinase from giardia lamblia. | the steady-state kinetics of the reaction catalyzed by inorganic-pyrophosphate-dependent d-fructose-6-phosphate 1-phosphotransferase from giardia lamblia have been investigated. the reactants for the forward and reverse reactions were the mg-chelated complexes of pyrophosphate (ppi) and pi. uncomplexed ligands were not substrates. in the direction of phosphorylation of fructose-6-phosphate (f6p), initial velocity double-reciprocal plots for both ppi and f6p were intersecting suggesting sequentia ... | 1995 | 8577346 |
| 'the tropics in our bathroom': chronic diarrhoea after return from the tropics. | interest in imported tropical diseases has increased with the rising number of travellers to the tropics. this is especially true in the case of tropical gastroenterologic disorders. the causative organisms of chronic diarrhoea are different from those causing acute diarrhoea. bacteria are relatively unusual; parasites, e.g. entamoeba histolytica or giardia lamblia or an opportunistic parasitic infestation associated with an hiv infection are more likely. furthermore, non-infectious causes, such ... | 1995 | 8578232 |