Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| diarrhea in one to three week-old piglets associated with clostridium perfringens type a. | investigation on the aetiology of diarrhoea in piglets of one to three weeks of age revealed high numbers of clostridium perfringens type a in intestinal contents of severely affected animals. experimental infections with hysterectomy derived, colostrum deprived piglets performed in an isolator resulted in a clinical picture indistinguishable from the clinical signs observed under field conditions i.e. creamy diarrhoea, emaciation, abundant gas in the gut but usually low mortality rate. the pred ... | 1983 | 6329060 |
| proteolysis of sialoglycoprotein by pasteurella haemolytica cytotoxic culture supernatant. | proteolytic enzyme activity releasing sialo glycopeptides from 3h-labeled human erythrocyte ghosts was detected in cytotoxic (leukotoxic) culture supernatants from 9 of 12 pasteurella haemolytica serotypes. microcrystalline cellulose thin-layer chromatograms of radioactive water-soluble products showed the following two radioactive peaks: a high-mobility minor peak (rf, 0.54 to 0.74), identified as sialic acid, and a low-mobility major peak (rf, 0.18 to 0.21), partially characterized as a trichl ... | 1983 | 6352504 |
| anaerobic bacteria as cause of infections in female genital organs. | anaerobic bacteria constitute a substantial component of the normal vaginal flora and of the outer cervical canal. consequently, one would expect infections emanating from the vaginal flora to be caused to a substantial degree by anaerobic bacteria. the anaerobes may contribute in colpitis, but their role is difficult to prove in this situation, since sampling only yields normal flora components. one clue that anaerobes may contribute to colpitis is the circumstance that the flora under those co ... | 1983 | 6353553 |
| comparison of three methods for anaerobe identification. | in this study we evaluated the ability of three commercial methods, api 20a (analytab products, plainview, n.y.), minitek (bbl microbiology systems, cockeysville, md.), and anaerobe-tek (flow laboratories, inc., mclean, va.), to accurately identify 165 recent clinical and 38 stock isolates of anaerobic bacteria without supplemental tests or gas-liquid chromatography. strains included 89 bacteroides spp., 12 fusobacteria, 10 gram-positive, nonsporing rods, 43 clostridium spp., 15 streptococcus in ... | 1983 | 6355150 |
| decreased [20-3h]phorbol-12,13-dibutyrate binding to phospholipase c pretreated epidermal cells. | incubation of mouse epidermal cells (hel-37) with phospholipase c (clostridium perfringens) caused about a 50% decrease in the specific binding of [20-3h]phorbol-12,13-dibutyrate. phospholipase c caused a decrease in the apparent number of binding sites from 2.86 x 10(5) to 1.21 x 10(5) per cell with little effect on ligand affinity. the decrease was observed with purified phospholipase containing no detectable protease activity, and when cells were fixed with glutaraldehyde. the phorbol-12,13-d ... | 1983 | 6573950 |
| binding of clostridium perfringens 125i-labeled delta-toxin to erythrocytes. | hemolytically active, 125i-labeled delta-toxin from clostridium perfringens was used to study the binding of this cytolysin to sheep, goat, human, rabbit, horse, mouse, and guinea pig erythrocytes. the extent of toxin binding was correlated with the known hemolytic specificity of the toxin. detailed studies of the binding were carried out on sheep erythrocytes which showed the highest sensitivity to lysis by delta-toxin. simultaneous determination of toxin binding and release of intracellular 86 ... | 1983 | 6822539 |
| clostridium perfringens cellulitis and immune-mediated hemolytic anemia in a horse. | a 10-year-old quarter horse mare was referred for evaluation and treatment of a large pectoral skin slough and hemoglobinuria. the skin slough was secondary to clostridium perfringens cellulitis and associated gas gangrene. cold hemagglutinin disease was diagnosed and was suspected to be secondary to c perfringens septicemia. the autoimmune hemolytic anemia, severe intravascular hemolysis, and hemoglobinuria were treated with dexamethasone and hydrocortisone. the infection was treated with 20 x ... | 1983 | 6826449 |
| bacteria in enteric lesions of cattle. | thirty-nine species of bacteria were isolated from or demonstrated in the abomasal and small and large intestinal mucosa of 23 adult cattle and 41 calves and identified. the bacteria isolated were related to the gross and microscopical lesions. campylobacters, clostridium perfringens type a, c sordellii, actinobacillus lignieresii, fusobacterium necrophorum, mycobacterium paratuberculosis, aeromonas hydrophila and escherichia coli were all associated with specific lesions. the relationship of ot ... | 1983 | 6829137 |
| photolabeling of staphylococcal alpha-toxin from within rabbit erythrocyte membranes. | intrinsic membrane proteins of rabbit red blood cells were labeled with the photoreactive amphipatic reagent 12-(4-azido-2-nitrophenoxy) stearoyl (1-14c) glucosamine, which inserts into the hydrophobic membrane region and generates a reactive nitrene upon ultraviolet irradiation. photolabeling of membrane-bound staphylococcal alpha-toxin after lysis of probe-treated rabbit red blood cells by this toxin implies its penetration into the hydrophobic region of the outer leaflet of the membrane. in c ... | 1983 | 6838569 |
| association of iota-like toxin and clostridium spiroforme with both spontaneous and antibiotic-associated diarrhea and colitis in rabbits. | a helically coiled, anaerobic, gram-positive sporeforming bacillus, identified as clostridium spiroforme, was isolated from the cecal contents of all of 27 rabbits with spontaneous diarrhea, at a mean concentration of 10(6.0) spores per g of material. all of these rabbits also had a toxin present in their cecal contents that was neutralized by anti-clostridium perfringens type e iota toxin, but not by other clostridial antitoxins. in addition, four rabbits with clindamycin-associated colitis wer ... | 1983 | 6841578 |
| myonecrotic gas gangrene of the extremities. | twenty-three patients with proven myonecrotic gas gangrene of the extremities caused by clostridium perfringens bacteria were treated by a combination of surgical measures, benzyl-penicillin and hyperbaric oxygen at three atmospheres. 87 per cent (20/23) of the cases were cured and half of the surviving patients (10) had to be amputated at least one level higher than on admission. no single factor could be established that influenced the course of the infection comparing age under and over 50 ye ... | 1983 | 6845998 |
| exogenous phospholipase enzymes mimic effects of phenylephrine on ca2+ transport in hepatocytes. | phospholipase c from clostridium perfringens induced the release of 45ca2+ from isolated rat hepatocytes incubated at 0.1 mm extracellular ca2+ with a time course similar to that for the action of phenylephrine. under the conditions of these experiments, no significant damage to the plasma membrane was detected in the presence of phospholipase c. little 45ca2+ release was induced by bee venom phospholipase a2. at 1.3 mm extracellular ca2+, both phospholipase enzymes stimulated the initial rate o ... | 1983 | 6847637 |
| effects of phospholipase c on the na+-ca2+ exchange and ca2+ permeability of cardiac sarcolemmal vesicles. | we have examined the effects of phospholipase c pretreatment on ca2+ transport in highly purified canine cardiac sarcolemmal vesicles. na+-ca2+ exchange, measured as nai+-dependent ca2+ uptake, is stimulated when 10-70% of the membrane phospholipid has been hydrolyzed. although the phospholipase c treatment also increases sarcolemmal passive ca2+ flux, the membrane maintains a sufficient permeability barrier for enhanced transport (via na+-ca2+ exchange) to be observed. these effects can be obta ... | 1983 | 6853558 |
| activation of the calcium permeability of erythrocyte membranes by perfringolysin o. | calcium ions inhibited perfringolysin o-induced hemolysis at a concentration lower than 1 mm, but not the hemolysis by digitonin at 10 mm. the introduction of calcium ions into ghosts inhibited the lysis more strongly than the addition of calcium ions outside ghosts. when erythrocytes were treated with perfringolysin o in the presence of 1 mm cacl2 containing 45cacl2, the radioactivities inside cells rapidly increased during incubation. on the other hand, when perfringolysin o-treated erythrocyt ... | 1983 | 6619118 |
| cefamandole-erythromycin-heparin peritoneal irrigation: an adjunct to the surgical treatment of diffuse bacterial peritonitis. | the enthusiasm for the use of peritoneal irrigation has waxed and waned since its introduction by dr. pierce in 1905. the purpose of this study was to devise a relatively low-cost irrigant that could be used for continuous intraperitoneal lavage, with the intent of decreasing abscess formation following surgical treatment for generalized bacterial peritonitis. a solution of 1 l of normal saline containing 50 mg erythromycin, 50 mg cefamandole, 500 u heparin, and 5 meq kcl was proven in in vitro ... | 1983 | 6623358 |
| freeze-dried mixed cultures as samples for proficiency tests and collaborative studies in food microbiology. | a method is presented for preparing samples of freeze-dried mixtures of microorganisms for proficiency tests and collaborative studies. the samples may include most microorganisms that are found in routine analysis in food laboratories. transport of samples during 48 h did not decrease the number of microorganisms, nor was the variability among samples significantly affected by transport. the standard deviation of counts after 5 weeks of storage varied from 0.04 (staphylococcus aureus) to 0.17 ( ... | 1983 | 6643366 |
| detection of d-erythro and l-threo sphingosine bases in preparative sphingosylphosphorylcholine and its n-acylated derivatives and some evidence of their different chemical configurations. | sphingosylphosphorylcholine prepared from native sphingomyelin by the kaller procedure was found to comprise about 70% of the l-threo (2s, 3s) isomer and 30% of the d-erythro (2s, 3r) isomer. this analytical result was obtained by gas-liquid chromatography (glc) of trimethylsilyl derivatives of n-acetylsphingosines which were prepared by enzymatic hydrolysis of synthetic n-acetylsphingosylphosphorylcholines with clostridium perfringens phospholipase c. some other evidence of the different chemic ... | 1983 | 6654881 |
| [anaerobic effect of tinidazol compared with metronidazol, ornidazol, cefoxitin and lamoxactam]. | the in vitro inhibitory activity of tinidazole, metronidazole, ornidazole, cefoxitin and moxalactam was determined against 150 isolates of clinically important anaerobes including bacteroides fragilis, bacteroides bivius and clostridium perfringens by means of agar dilution tests. the members of 18 gramnegative and 14 grampositive species were inhibited by tinidazole at less than or equal to 0,01-8 micrograms/ml thus being without exception susceptible to the drug. a similar in vitro activity wa ... | 1983 | 6676177 |
| in vitro activity of u-57930e against anaerobic bacteria and its comparison with clindamycin, ampicillin, carbenicillin and tetracycline. | the in vitro activity of u-57930e, a pipecolic acid amide of clindamycin, was compared with those of clindamycin, ampicillin, carbenicillin and tetracycline against 321 anaerobic clinical isolates. the mic (micrograms/ml) of u-57930e that inhibited 95% bacteroides fragilis, peptococcus prevotii, b. melaninogenicus and p. asaccharolyticus was 0.0625; 0.03125 for peptostreptococcus anaerobius, b. vulgatus, propionibacterium and peptococcus species. clindamycin, on the other hand, gave mic values o ... | 1983 | 6678915 |
| [efficacy of tinidazole against anaerobes in comparison with metronidazole, ornidazole, cefoxitin and lamoxactam]. | the in vitro inhibitory activity of tinidazole, metronidazole, ornidazole, cefoxitin and moxalactam was determined against 150 isolates of clinically important anaerobes including bacteroides fragilis, bacteroides bivius and clostridium perfringens by means of agar dilution tests. the members of 18 gramnegative and 14 grampositive species were inhibited by tinidazole at less than or equal to 0,01-8 micrograms/ml thus being without exception susceptible to the drug. a similar in vitro activity wa ... | 1983 | 6681175 |
| phospholipids chiral at phosphorus. preparation and spectral properties of chiral thiophospholipids. | the thiophospholipid 1,2-dipalmitoyl-sn-glycero-3-thiophosphocholine (dppsc) was shown to be a mixture of two diastereomers by 31p nuclear magnetic resonance. the isomer that resonates at the lower field in cdcl3 (56.12 ppm) was designated as isomer a and the other (resonates at 56.07 ppm) as isomer b. phospholipase a2 from four different sources (bee venom, naja naja venom, crotalus adamanteus venom, and porcine pancreas) was shown to hydrolyze the isomer b of dppsc specifically, whereas phosph ... | 1983 | 6688028 |
| effect of erythromycin on microbial antagonisms: a study in gnotobiotic mice associated with a human fecal flora. | the effect of erythromycin base was studied on intestinal resistance to colonization of gnotobiotic mice inoculated with a human fecal flora and challenged with six microbial strains potentially pathogenic for immunocompromised patients. fecal concentrations of erythromycin were greater than 1,000 micrograms/g in the human donor and in mice. total intestinal bacterial counts were not significantly different in the human donor and in the recipient mice and were not affected by erythromycin treatm ... | 1983 | 6413596 |
| pigbel in papua new guinea: an ancient disease rediscovered. | a hypothesis suggested in this paper is that pigbel, or enteritis necroticans was a common disease in mediaeval europe when human habitats, food hygiene, protein deficiency and periodic meat feasting formed the basics of village life as they do in many third world cultures today. based on the papua new guinea experience with pigbel, it is suggested that health authorities should look closely at the epidemiology of the acute surgical abdomen in such communities. enteritis necroticans may be the i ... | 1983 | 6307898 |
| a nosocomial outbreak of food poisoning caused by enterotoxigenic clostridium perfringens. | 1983 | 6308401 | |
| [personal experience with the diagnosis and treatment of anaerobic infections in surgery]. | 1983 | 6308837 | |
| the use of radiolabelled triostin antibiotics to measure low levels of binding to deoxyribonucleic acid. | triostin antibiotics, which contain a cyclic peptide with a disulphide bridge, have been prepared by growing streptomyces triostinicus in the presence of inorganic [35s]-sulphate. the labelled triostin a has been shown to behave in all respects similarly to the authentic natural product and to enable a much more sensitive radiochemical adaptation of the solvent-partition method for determining antibiotic binding to dna. by this means, binding isotherms at low, biologically relevant levels (down ... | 1983 | 6309135 |
| enterotoxemia in the goat: the humoral response and local tissue reaction following vaccination with two different bacterin-toxoids. | a vaccination trial involving 72 goats was designed to compare the epsilon antitoxin titres and local reactions at the injection sites, of two commercial enterotoxemia vaccines. three dosage regimens were used for each vaccine (12 goats per group). although no significant differences were noted in humoral immune response between the two vaccines (p = 0.05), one vaccine regime resulted in low titres (p = 0.05) on two occasions. local tissue reactions at injection sites persisted for six months in ... | 1983 | 6309346 |
| isolation and characterization of the trisialogangliosides from bovine adrenal medulla. | trisialogangliosides were isolated from bovine adrenal medulla by deae-sephadex a-25 and iatrobeads column chromatography. their structures were elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. the complete structures of trisialogangliosides, a to d, were identified as follows. a: gt1b, iv3neuac, ii3 (neuac)2-ggose4cer. b: gt1b(neuac/neuac-neugc-); iv3neuac, ii3 (neuac alpha 2-8 neugc-)ggose4cer. c: gt1b (neugc/neuac-neuac-); iv3neugc, ii3 (neuac alpha 2-8 neuac ... | 1983 | 6310012 |
| isolation of clostridium perfringens from the canine genital tract. | 1983 | 6310848 | |
| quantification of bovine igg, igm and iga antibodies to clostridium perfringens b-toxin by enzyme immunoassay i. preparturient immunization for enhancement of passive transfer of immunity. | a quantitative competitive binding "triple-sandwich" enzyme immunoassay was developed and used to evaluated pathogen/class-specific antibody responses in holstein-friesian cows vaccinated against clostridium perfringens b-toxin. vaccination of cows at six weeks and again at two weeks prepartum increased pathogen-specific igg levels in each dam's colostrum and respective calf's serum. pathogen-specific igg and igm concentrations in dams' sera and colostra were related to subsequent pathogen-speci ... | 1983 | 6310852 |
| quantification of bovine igg, igm and iga antibodies to clostridium perfringens b-toxin by enzyme immunoassay ii systemic effects of maternally derived antibodies on immunization of newborn calves. | a quantitative competitive binding "triple sandwich" enzyme immunoassay was used to evaluate pathogen/class-specific antibody responses in forty-eight holstein-friesian calves vaccinated against clostridium perfringens b-toxin at various ages postpartum. to assure a wide serum pathogen/class-specific immunoglobulin concentration range, one-half of all calves were from previously vaccinated dams. calves initially vaccinated at three days produced both a primary and secondary pathogen-specific ant ... | 1983 | 6310853 |
| conditions for production of initiation protein for germination of clostridium perfringens spores. | the amount of initiation protein (ip) produced by clostridium perfringens was increased by approximately one-third by modification of duncan-strong sporulation medium to include 0.5% glucose, 2% proteose peptone, and 2% sodium phosphate. ip but no spores were produced by the wild type when grown at 46 degrees c and by a mutant blocked at stage 0 of sporulation. ip appears to be a vegetative cell product, perhaps an overproduced autolysin or pathogenicity factor. | 1983 | 6311387 |
| selective release of the disaccharide 2-acetamido-2-deoxy-3-o-(beta-d-galactopyranosyl)-d-galactose from epiglycanin by endo-n-acetyl-alpha-d-galactosaminidase. | epiglycanin, the major glycoprotein of ta3-ha mammary carcinoma ascites cells, was radiolabeled with tritium in the terminal d-galactose and 2-acetamido-2-deoxy-d-galactose residues. alkaline-borohydride treatment, reported to release five o-glycosyl-linked chain types from epiglycanin, resulted in the cleavage of 98-99% of the radioactivity from the protein. of this, 63% of the radioactivity from epiglycanin and 70% from asialoepiglycanin co-migrated with an authentic sample of 2-acetamido-2-de ... | 1983 | 6311416 |
| analysis of the common polysaccharide antigens from the cell envelope of clostridium perfringens type a. | the major, common antigen of clostridium perfringens type a, isolated and purified independently from three selected strains (hobbs 5, hobbs 9, and hobbs 10), is composed of equimolar amounts of 2-acetamido-2-deoxy-d-mannose (man-nac) and 2-acetamido-2-deoxy-d-glucose (glcnac). the purified antigen gave a strong immunoprecipitin line by double immunodiffusion in gel. smith degradation of the major, common antigen caused decomposition of all of the glcnac, without concomitant loss in mannac, or a ... | 1983 | 6311417 |
| [prevention of anaerobic infections of ruminants in madagascar by intradermal administration of ultrafiltered toxoids of locally specific clostridia]. | 1983 | 6311508 | |
| investigation of the survival characteristics of rhodococcus coprophilus and certain fecal indicator bacteria. | rhodococcus coprophilus and clostridium perfringens survived in fresh water samples held at 5, 20, and 30 degrees c for over 17 weeks, whereas escherichia coli and fecal streptococci disappeared after 5 weeks at all three temperatures. r. coprophilus survived for more than 8 months in sterilized sewage and deionized water at all three temperatures, whereas in normal sewage held at 20 degrees c, the survival time was 12 to 26 weeks. in samples held at 30 degrees c, survival times were shorter, pr ... | 1983 | 6312883 |
| comparison of iron milk and official aoac methods for enumeration of clostridium perfringens from fresh seafoods. | fresh seafoods collected from seattle area markets were evaluated for the presence and numbers of clostridium perfringens. in a comparison of the official aoac shahidi-ferguson perfringens and the simple iron milk methods, 29 of 287 samples of fresh fish and shellfish were positive for c. perfringens. all 29 positive samples were detected by the iron milk medium; however, only 7 of the 29 were detected by the aoac methods. a statistical evaluation of the data showed a significant difference betw ... | 1983 | 6313601 |
| resistance of clostridium perfringens to beta-lactam antibiotics mediated by a decreased affinity of a single essential penicillin-binding protein. | benzylpenicillin-resistant mutants of clostridium perfringens have been isolated by in vitro selection. the sole mechanism of resistance was a decreased affinity of the highest molecular weight penicillin-binding protein (pbp1) for the antibiotic. | 1983 | 6313857 |
| enteritis necroticans in the southern highlands of papua new guinea. | 1983 | 6313956 | |
| in vitro and in vivo evaluations of the antibiotic efrotomycin. | experiments were conducted to determine the potential of the antibiotic efrotomycin as a growth permittant for poultry and to further elucidate the mode of action of antimicrobial agents for that purpose. efrotomycin as the semipurified antibiotic and as fermentation solids demonstrated excellent activity against clostridium perfringens at .1 to .2 ppm based on suppression of gas production in an anaerobic tube test. supplementing a soybean protein and sucrose-based diet with levels of 2.2, 11, ... | 1983 | 6314312 |
| effects of diet and bacitracin on growth, feed efficiency, and populations of clostridium perfringens in the intestine of broiler chicks. | experiments were conducted to demonstrate the activity of bacitracin as a growth permittant for poultry and to further elucidate the mode of action of antimicrobial agents for that purpose. supplementing a soybean protein and sucrose-based diet with 2.2, 11, and 55 ppm of bacitracin resulted in significant improvements in weight gain and feed efficiency of chicks fed the higher levels. in a second experiment, graded levels of bacitracin from 1.1 to 55 ppm were fed. a regression analysis of the i ... | 1983 | 6314313 |
| effect of the antibiotic thiopeptin on clostridium perfringens and growth and feed efficiency of broiler chicks. | experiments were conducted to determine the potential of the antibiotic thiopeptin as a growth permittant for poultry. thiopeptin as the semipurified antibiotic and as fermentation solids demonstrated activity against clostridium perfringens at .03 ppm based on suppression of gas production in an anaerobic tube test. supplementing a soybean protein and sucrose-based diet with levels of 2.2, 11, and 55 ppm of thiopeptin from each of the two sources resulted in significant improvements in weight g ... | 1983 | 6314314 |
| [in vitro sensitivity of clostridium perfringens to 11 antimicrobial drugs]. | 1983 | 6314443 | |
| [an outbreak of clostridium perfringens enteritis in suckling piglets in switzerland]. | 1983 | 6314500 | |
| [determination of the enzymatic traits of anaerobic gram-negative nonsporulating bacteria]. | the method requiring the use of a semi-liquid medium with the reduced content of protein hydrolysates and with bromo-thymol blue added as indicator and permitting the determination of sugar fermentation by bacteroids and fusobacteria is presented. the determination is made by a change in the color of the indicator and the intensity of microbial growth (opacity) after the incubation of the inoculated medium under free access of air. a study has been made for comparing the above method with the el ... | 1983 | 6314710 |
| [determination of in vivo and in vitro toxin activity. i. a new method of determining in vitro activity]. | the potency of 5 toxins of different microbial species has been studied by common in vivo methods and by the in vitro method based on measuring the relative test-microbe growth inhibiting units 50. the possibility of using this method for the determination of toxicity in vitro with a view of studying the potency of diphtheria and gas-gangrene exotoxins, as well as that of pseudomonas aeruginosa exotoxin, is substantiated. | 1983 | 6314713 |
| [comparative evaluation of the methods of determining perfringens a antitoxin]. | the methods for determining the level of type a perfringens antitoxin in human blood sera were examined and compared. the ratios for correlating the data obtained in the toxin neutralization test (nt) in vivo, in the passive hemagglutination test (pht), and as a result of the enzyme-labeled immunosorbent assay (elisa) with regard to the antitoxin level measured in the nt in vitro were equal to 0.88, 0.64 and 0.39, respectively. the sensitivity of the nt in vivo and in vitro was 0.25 iu/ml, that ... | 1983 | 6315110 |
| preparation of neuraminidase-resistant human alpha 1-protease inhibitor and its clearance in rat blood circulation. | the sialic acid residues of human alpha 1-protease inhibitor were modified by periodate oxidation and subsequent reductive amination with ethanolamine and sodium cyanoborohydride. the modified inhibitor retained its original trypsin inhibitory activity and was not digested by neuraminidase from clostridium perfringens. the modified inhibitor disappeared from rat blood circulation at the same rate as the native inhibitor. | 1983 | 6315502 |
| increase in osmotic fragility of bovine erythrocytes induced by bacterial phospholipases c. | bovine erythrocytes were treated with each of three bacterial phospholipases c; phosphatidylcholine-hydrolyzing phospholipase c (pcase) of clostridium perfringens, sphingomyelinase c (smase) of bacillus cereus and phosphatidylinositol-specific phospholipase c (piase) of bacillus thuringiensis. an increase in osmotic fragility was detected by means of a coil planet centrifugation (cpc) apparatus (biomedical systems co., tokyo) after the treatment with these enzymes. the peak of hemolysis normally ... | 1983 | 6302097 |
| studies on the interactions between phospholipids and membrane-bound enzymes in microsomes. effects of phospholipases c on kinetic properties of the glucose-6-phosphatase system in rat liver microsomes. | through kinetic analysis, the relationships between the glucose-6-phosphatase system and constituent phospholipids were studied in rat liver microsomes. when phosphoglycerides such as phosphatidylcholine and phosphatidylethanolamine on the microsomal membrane were hydrolyzed by phospholipase c of c. perfringens, the activities of glucose-6-p phosphohydrolase and glucose-6-p:glucose phosphotransferase both decreased with or without subsequent exposure to taurocholate. in these cases, the michaeli ... | 1983 | 6302099 |
| detection of clostridium perfringens enterotoxin by tissue culture and double-gel diffusion methods. | culture filtrates of four enterotoxin-producing strains of clostridium perfringens type a produced morphological changes in cells of 10 of 12 established mammalian cell lines tested, and particularly vero (african green-monkey kidney) and mdck (dog kidney) cells. double-gel diffusion and vero-cell tests detected enterotoxin in culture filtrates of, respectively, 29 (28%) and 56 (54%) of 103 strains of c. perfringens tested. the feasibility of cell-culture assays for the routine detection of c. p ... | 1983 | 6302258 |
| configuration of substrate and products of n-acetylneuraminate pyruvate-lyase from clostridium perfringens. | to characterize the true substrate for aldolase from clostridium perfringens (optimum ph = 7.2) several experiments were carried out wherein the substrate neu5ac was generated in situ at ph 5.4 by the action of sialidase on its substrate neu5ac(alpha, 2 leads to 3) lactose. the alpha-anomer formed in this reaction was found to be split by aldolase at this ph into mannac and pyruvate. beta-neu5ac as such was not converted at ph 5.4. however, when it was first mutarotated until the equilibrium mix ... | 1983 | 6301475 |
| the specificity of viral and bacterial sialidases for alpha(2-3)- and alpha(2-6)-linked sialic acids in glycoproteins. | the anomeric specificity of six sialidases (vibrio cholerae, arthrobacter ureafaciens, clostridium perfringens, newcastle disease virus, fowl plague virus and influenza a2 virus sialidases) was assessed with sialylated antifreeze glycoprotein, ovine submandibular gland glycoprotein and alpha 1-acid glycoprotein, resialylated specifically in alpha(2-3) or alpha(2-6) linkage with n-acetylneuraminic acid or n-glycolylneuraminic acid using highly purified sialyltransferases. the rate of release of s ... | 1983 | 6301560 |
| vitamin e-enhanced humoral antibody response to clostridium perfringens type d in sheep. | 1983 | 6301619 | |
| susceptibility of 50 isolates of clostridium perfringens to cefotaxime, fosfomycin, penicillin g and vancomycin; variable tolerance for vancomycin. | 50 strains of clostridium perfringens recovered from human fecal specimens were examined for susceptibility to cefotaxime (ctx), fosfomycin (fosfo), penicillin g (pen g) and vancomycin (vanco) with the aid of a microtiter procedure. the geometric mean minimal inhibitory concentrations (mics) of ctx, fosfo, pen g and vanco were 0.81, 48.3, 0.15 and 0.33 micrograms/ml, respectively; the geometric mean bactericidal concentrations (mbcs) of the four drugs were 1.25, 109.8, 0.53 and 9.05 micrograms/m ... | 1983 | 6301777 |
| identification of a 50,000 mr protein from rabbit brush border membranes that binds clostridium perfringens enterotoxin. | a protein that binds clostridium perfringens enterotoxin was extracted with np-40 from rabbit intestinal brush border membranes. this protein was partially purified by affinity chromatography on enterotoxin-coupled cnbr-activated sepharose 4b. the molecular weight of this protein was approximately 50,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. affinity-purified samples containing this protein specifically inhibited biological activity of the enterotoxin on ver ... | 1983 | 6303335 |
| empyema caused by clostridium perfringens. | trauma, chest surgery or other invasive procedures and underlying lung disease are often found to precede clostridial empyema. a case is described in which empyema caused by clostridium perfringens was not associated with any of these factors. | 1983 | 6303544 |
| [investigations on an outbreak of clostridium perfringens food poisoning]. | 1983 | 6303724 | |
| sequence of the amino-terminal part of enterotoxin from clostridium perfringens type a: identification of points of trypsin activation. | the sequence of the first 66 amino acids of the amino-terminal part of the enterotoxin from clostridium perfringens type a is presented. the trypsin activation of the enterotoxin involves hydrolysis of lys15-glu16 and lys25-thr26 bonds. the n-terminal sequence of the trypsin-activated enterotoxin has limited homology with the sequence of the n-terminal region of the cholera toxin b subunit. | 1983 | 6303961 |
| a note on the antibiotic susceptibilities of clostridium perfringens serotypes isolated from meat. | 1983 | 6303997 | |
| mechanism of chemical manipulation of the heat resistance of clostridium perfringens spores. | the mechanism of chemical manipulation of the heat resistance of clostridium perfringens type a spores was studied. spores were converted to various ionic forms to base-exchange technique and these spores were heated at 95 degrees c. of the four ionic forms, i.e. ca2+, na+, h+ and native, only hydrogen spores appeared to have been rapidly inactivated at this temperature, when survivors were enumerated on the ordinary plating medium. however, the recovery of the survivors was improved when the pl ... | 1983 | 6303999 |
| recurrent diarrhoea in a dog associated with clostridium perfringens type a. | a case of chronic intermittent diarrhoea in a dog is reported. large numbers of clostridium perfringens type a were recovered from the faeces. a toxin neutralised by c perfringens type a antitoxin was demonstrated in the same samples. no clear predisposing factors contributing to the condition could be determined. the dog recovered following a 10 day course of metronidazole at 20 mg/kg/day per os and a change to a bland diet. | 1983 | 6304987 |
| acute clostridial enteritis--or pig-bel? | acute postprandial gastric dilatation and associated clostridial enteritis necroticans is a well recognised but unusual clinical condition. non-pathological overeating, gastric distension, and clostridial enteritis, termed pig-bel, has been reported in papua, new guinea. a similar condition (darmbrand) was reported from germany after the second world war, but it is not a condition seen in our society today. gastric dilatation alone may be seen in individuals with anorexia nervosa, who may occasi ... | 1983 | 6305781 |
| 'gas gangrene' after a below-knee amputation. | 1983 | 6305823 | |
| evidence for mucins and sialic acid as receptors for pseudomonas aeruginosa in the lower respiratory tract. | the nature of the receptors for mucoid and nonmucoid pseudomonas aeruginosa was investigated by using adherence to injured tracheal epithelium as a model. bovine submaxillary mucin and crude rat tracheal mucin inhibited the adherence of both types of p. aeruginosa. among the sugars present in these mucins only n-aceylneuraminic acid inhibited adherence. inhibition of adherence probably involved the binding of n-acetylneuraminic acid to the bacterial cells and not to the tracheal cells. the mucoi ... | 1983 | 6305842 |
| induction of ornithine decarboxylase by treatment of guinea pig lymphocytes with phospholipase c. | treatment of guinea pig lymphocytes with clostridium perfringens phospholipase c but not with naja naja snake venom phospholipase a2 increased ornithine decarboxylase activity. the increase in ornithine decarboxylase activity was suppressed by actinomycin d or cycloheximide, suggesting that de novo syntheses of rna and protein are necessary for the increase in the enzyme activity. these results suggest that the activation of phospholipase c rather than that of phospholipase a2 is responsible for ... | 1983 | 6299785 |
| [emphysematous cholecystitis caused by clostridium perfringens. sonographic and computer tomographic findings]. | 1983 | 6299914 | |
| neonatal diarrhea of pigs in quebec: infectious causes of significant outbreaks. | to evaluate the relative importance of the various enteropathogens causing neonatal diarrhea in quebec farrowing operations, observations were made on 749 diarrheic pigs from 325 outbreaks of diarrhea. they were one to 15 days of age, and were obtained alive for necropsy generally within 48 hours of the onset of diarrhea. some pigs were from severe, explosive outbreaks of diarrhea with high morbidity and mortality rates, while others were from herds with chronic neonatal diarrhea with lower morb ... | 1983 | 6299483 |
| immunisation schedule for the prevention of infectious necrotic enteritis caused by clostridium perfringens type c in piglets. | 1983 | 6301137 | |
| flow dichroism evidence for tilting of the bases when dna is in solution. | flow linear dichroism studies of dnas from clostridium perfringens, escherichia coli, and micrococcus luteus are extended into the vacuum uv region to 175 nm. the short-wavelength pi pi region shows a less negative reduced linear dichroism than the well-studied long-wavelength pi pi region. these results are independent of the type of dna and the concentration of salt over the range 0.001 to 1.0 m. this proves that the bases are not perpendicular to the flow orientation. assuming that the dna te ... | 1983 | 6300899 |
| clostridium perfringens and other anaerobes isolated from bile. | clostridium perfringens was isolated from bile in 13 cases of 150 patients examined. the serotypes of c perfringens strains isolated from bile and faeces were investigated using antisera to hobbs' type 1-17. two or more serological types were often found in a single specimen, but in the same patient the serotypes of c perfringens strains isolated from the bile were identical with those from the faeces. beta-glucuronidase production in these c perfringens serotypes was tested with the api-strep s ... | 1983 | 6298284 |
| sendai virus envelopes can mediate epstein-barr virus binding to and penetration into epstein-barr virus receptor-negative cells. | epstein-barr virus (ebv) receptor-negative cells were treated with uv-inactivated sendai virus (sv) or with reconstituted sv envelopes having a low hemolytic activity and then assayed for ebv binding or for susceptibility to ebv infection. ebv binding was assessed by using both unlabeled and fluoresceinated ebv preparations. it was found that sv or sv envelope treatment renders these cells able to bind ebv. various experiments were performed to clarify the mechanism of this sv-induced binding. t ... | 1983 | 6298467 |
| clostridial infection of a perinephric hematoma. | 1983 | 6298473 | |
| food poisoning due to clostridium perfringens in the united states. | 1983 | 6296240 | |
| spontaneous bacterial peritonitis and pneumoperitoneum. a false surgical emergency. | 1983 | 6296474 | |
| regulation of phosphatidylcholine biosynthesis in mammalian cells. i. effects of phospholipase c treatment on phosphatidylcholine metabolism in chinese hamster ovary cells and lm mouse fibroblasts. | addition of phospholipase c from clostridium perfringens to cultures of chinese hamster ovary (cho) cells resulted in rapid degradation of cellular phosphatidylcholine with concomitant release of phosphocholine. the rate of incorporation of radiolabeled choline into lipids was increased 2-fold in phospholipase c-treated cho cells as compared to untreated controls. the only enzyme in the pathway of phosphatidylcholine biosynthesis with increased activity in phospholipase c-treated cells was ctp:p ... | 1983 | 6296084 |
| regulation of phosphatidylcholine biosynthesis in mammalian cells. ii. effects of phospholipase c treatment on the activity and subcellular distribution of ctp:phosphocholine cytidylyltransferase in chinese hamster ovary and lm cell lines. | ctp:phosphocholine cytidylyltransferase was located in both the cytosolic and particulate fractions from chinese hamster ovary cells. the activity of the cytosolic form of the enzyme was greatly enhanced by incubation with sonicated preparations of several different lipids, although incubations with either phosphatidylcholine or 1,2-sn-diolein did not increase activity. the activation of the cytidylyltransferase in chinese hamster ovary cells treated with phospholipase c from clostridium perfrin ... | 1983 | 6296085 |
| [staphylococcal coagglutination for the detection of the enterotoxin of clostridium perfringens]. | the staphylococcal coagglutination technique has been used in recent years for detecting various bacterial antigens and toxins. it is a simple and rapid technique which requires no specialized equipment. this report describes a staphylococcal coagglutination procedure for assaying c. perfringens enterotoxin. its sensitivity and specificity were studied with the aid of various enterotoxin preparations and culture filtrates of c. perfringens and other clostridium species. this assay detects betwee ... | 1983 | 6101068 |
| pyruvoyl-dependent histidine decarboxylases from clostridium perfringens and lactobacillus buchneri. comparative structures and properties. | histidine decarboxylase (ec 4.1.1.22) was purified to homogeneity from clostridium perfringens and also from lactobacillus buchneri. both enzymes are composed of alpha and beta subunits, with an essential pyruvoyl group bound to the alpha subunit. in this respect and also in molecular weight of both the alpha and beta subunits and the native enzyme, they closely resemble the previously described (riley, w.d., and snell, e. e. (1970) biochemistry 9, 1485-1491) histidine decarboxylase from lactoba ... | 1983 | 6294108 |
| establishment of minimum inhibitory concentrations of cefoperazone for control and reference anaerobic organisms. | the minimum inhibitory concentrations of cefoperazone were determined in a collaborative study for eight national committee for clinical laboratory standards control and reference strains of anaerobic bacteria by agar and microdilution techniques with several types and sources of media. recommended minimum inhibitory concentrations for the control strains, bacteroides fragilis atcc 25285 and bacteroides thetaiotaomicron atcc 29741, are 32 to 64 micrograms/ml and 64 micrograms/ml, respectively. c ... | 1983 | 6222065 |
| teichomycin: in-vitro and in-vivo evaluation in comparison with other antibiotics. | teichomycin, a new glycopeptide antibiotic with a spectrum of activity similar to that of vancomycin, was highly active against staphylococci, streptococci and gram-positive anaerobes (propionibacterium acnes, clostridium perfringens and cl. difficile). ninety per cent of the staphylococcus aureus and streptococcal strains, including enterococci, were inhibited by 0.4 mg/l; 90% of staph. epidermidis strains were susceptible to 1.6 mg/l. vancomycin was less active than teichomycin against all cli ... | 1983 | 6223907 |
| immunomodulation of primary antitoxic-type immunity with inactivated brucella suspensions. | 1983 | 6234766 | |
| ganglioside-basic protein interaction: protection of gangliosides against neuraminidase action. | the ability of acidic phospho- and sphingolipids to interact with basic proteins was studied by double diffusion analysis. the phospholipids, tri- and diphosphoinositide, and the sphingolipid, sulfatide, interacted with myelin basic protein as evidenced by precipitin line formation. of the sialoglycosphingolipids (gangliosides) tested, only the myelin-specific monosialoganglioside, gm4, formed a precipitin line with myelin basic protein. in addition, myelin basic protein retarded the activity of ... | 1983 | 6192246 |
| allogenic modification of rat sarcoma cells in vitro by staphylococcal exotoxin. | the effect of toxin preparations from clostridium welchii and staphylococcus aureus on the growth of some experimental animal tumor cell lines was investigated. while clostridium toxin exerted considerable cytotoxicity, it did not influence either in vivo or in vitro growth of recovered cells. however, staphylotoxin treated sarcoma cells, while showing normal in vitro growth and metabolism, exhibited decreased growth rates when transplanted into susceptible hosts. this effect was demonstrable ev ... | 1983 | 6197664 |
| bacteriocinogenic plasmids developments and recent insights. | 1983 | 6201152 | |
| [direct immunofluorescence reaction for the detection of the causative agent of anaerobic gas infection]. | 1983 | 6201663 | |
| combination chemotherapy with clostridium perfringens phospholipase c and cytosine antimetabolites: complementary inhibition directed at membrane lipids. | tumor cell membranes were susceptible to the action of clostridium perfringens phospholipase c, and this was reflected by inhibition of cellular replication in culture. the differential susceptibility of two neoplastic cell lines to this enzyme was studied in detail. the growth of sarcoma 180 cells cultured in fischer's medium was markedly inhibited by phospholipase c; whereas, in contrast, cultured l1210 leukemia cells were relatively resistant to the cytotoxic effects of this enzyme. the diffe ... | 1983 | 6085755 |
| [recognition of the pathogenesis of enteritis caused by type a clostridium perfringens]. | 1983 | 6093271 | |
| on the disposition of phospholipids in freshly isolated myelin sheath preparations from bovine brain. | the extent of availability of the four major classes of phospholipids in freshly isolated bovine brain myelin has been studied using phospholipase c (clostridium perfringens), phospholipase a (bee venom), sphingomyelase (staphalococcus aureus) and trinitrobenzenesulphonate. the results have been compared with those of a previous study (gwarsha et al., 1984). taken together, the data suggest that 47-67% of the phosphatidylcholine, 45-66% of the ethanolamine-containing phosphoglycerides, 52-55% of ... | 1984 | 20488087 |
| clinical models for anaerobic bacterial infections in dogs and their use in testing the efficacy of clindamycin and lincomycin. | two canine models of clinical anaerobic bacterial infections were developed for the study of the clinical parameters associated with these infections and for evaluation of antimicrobial agents that might be useful in therapy. in model i, a mixed culture of bacteroides fragilis, b melaninogenicus, and fusobacterium necrophorum was used as the inoculum. in model ii, a mixed culture of b fragilis and clostridium perfringens combined with an infection enhancer (sterile cinder dust) was used as the i ... | 1984 | 24049887 |
| carcinoma of the colon presenting as pulmonary microabscesses and septicaemia due to clostridium perfringens. | 1984 | 6093279 | |
| [anaerobic dysentery in pigs]. | anaerobic dysentery (necrotic enteritis) of sucking pigs was demonstrated on an industrial swine-breeding farm. the disease was found to be caused by clostridium perfringens, type c, and attacked up to 3 per cent of all sucking pigs at the age of 1 to 5 days, mortality rate ranging as high as 50 per cent. outbreaks were recorded only in boxes fixed on the floor of the premise itself. the control of the disease made use of complex measures, including washing of sows with disinfection solution pri ... | 1984 | 6093346 |
| an affinity technique for the isolation of clostridium perfringens type c from man and pigs in papua new guinea. | necrotising enteritis caused by clostridium perfringens type c (cwc) is an important cause of morbidity and death in highland regions of papua new guinea (png) and the organism is readily isolated from clinical cases. although detected in fluorescent antibody stains of faecal cultures, cwc has previously only once been isolated from normal people or from pigs in png. the difficulty results from the greater numbers of the much more common cl. perfringens type a (cwa) which is morphologically and ... | 1984 | 6094417 |
| spontaneous clostridial empyema and pyopneumothorax. | five patients developed pleural empyema due to clostridium perfringens in the absence of penetration of the thorax; two of the patients presented with pyopneumothorax. thirteen additional cases from the literature are reviewed. predisposing factors to the development of pleural empyema appear to include aspiration pneumonia, pulmonary embolization and infarction, and bacteremia from other foci. pleural disease and pulmonary tuberculosis may also predispose patients to pleural empyema. treatment ... | 1984 | 6095404 |
| common action of certain viruses, toxins, and activated complement: pore formation and its prevention by extracellular ca2+. | haemolysis by sendai virus, alpha-toxin, and activated complement is inhibited by high concentrations of divalent cations. in daudi cells, sublytic amounts of these agents induce the following changes: collapse of surface membrane potential, uptake of na+ and loss of k+ from cells, and leakage of phosphorylated metabolites from cells. the changes induced by sendai virus and complement are sensitive to physiological concentrations of extracellular ca2+. it is concluded that fluctuations in plasma ... | 1984 | 6095941 |
| [h-nmr spectroscopy. specificity of microbial sialidases against complex substrates]. | the specificities of one viral and five bacterial sialidases were investigated by 1h-nmr-spectroscopy with substrates or substrate mixtures containing two sialic acid residues of different linkage types. this technique allows - in contrast to the methods used before - the simultaneous determination of the rates of hydrolysis of both neuac linkages in a single experiment. the substrate specificities of the enzymes are discussed on the basis of the relation of the rate constants k/k'. the data obt ... | 1984 | 6096253 |
| biochemical properties of clostridium perfringens type a strains isolated from various sources. | 1984 | 6096268 | |
| substrate specificity of cholylglycine hydrolase for the hydrolysis of bile acid conjugates. | the substrate specificity of cholylglycine hydrolase has been investigated using bile acid conjugates with modifications in the steroid ring system, the side chain, or the amino acid moiety. epimerization at c-3 and c-7 did not affect the activity of the enzyme while oxidation of the three nuclear hydroxyl groups reduced the affinity of the enzyme toward the substrate. elongation of the side chain by one or three carbons inhibited enzyme activity. conjugates prepared from c24 bile acids and anal ... | 1984 | 6096355 |
| experimental and spontaneous clostridial enteropathies of laboratory and free living lagomorphs. | the enteric diseases of hares, european and cottontail rabbits, which are caused by members of the genus clostridium are reviewed. disease caused by c. perfringens types a and e, c. spiroforme, c. difficile, c. sordellii, c. tympany cuniculi and clostridial enterotoxins are included. tyzzer's disease also is discussed. | 1984 | 6096630 |
| the iron milk most probable number method for enumeration of clostridium perfringens in the diet and the intestine of the chick. | seven experiments were conducted to evaluate the iron milk most probable number method for enumeration of clostridium perfringens in the diet and the intestine of broiler chicks. levels of 50 ppm of neomycin and 20 ppm of polymixin improved the iron milk tube method when compared to the tsn (tryptone-sulfite-neomycin) agar plate method. low numbers of the organism, approximately 5 per gram, were detected in the practical diet fed to the chicks. vegetative cell numbers of c. perfringens increased ... | 1984 | 6096841 |