Publications

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antigenic properties of bacillus licheniformis cell wall components. 19714994588
velocity of chromosome replication in thymine-requiring and independent strains of bacillus subtilis.chromosomes in spores of a thymineless mutant of bacillus subtilis strain 168 were shown to have a replication fork, unlike chromosomes in spores of the thy(+) strain which are in a complete form. as a consequence the number of replication forks in germinating cultures is higher in the thy(-) strain than in the thy(+) one. chromosome replication time (c) in the thy(+) strain was found to be about 53 min for growth rates from 20 to 60 min. in the thy(-) strain, c was about 75 min at high growth r ...19714994603
[analysis of microbiological systems by means of reaction kinetics]. 19714994628
metabolic interlock. the role of the subordinate type of enzyme in the regulation of a complex pathway. 19714996881
nuclear and cell division in filamentous bacteria. 19714996896
independence of cell division and dna replication in bacillus subtilis. 19714996897
"alcalase" and serum proteins. 19714996789
[some data on somatic antigens of bac. anthracis]. 19714996428
cell walls of a teichoic acid deficient mutant of bacillus subtilis. 19714996443
binding of erythromycin to escherichia coli ribosomes. 19714996450
initial temperature sensitivity of streptomycin-dependent and tryptophan transformants of bacillus subtilis. 19714996512
transcription of nonrepeated dna in mouse brain.under normal conditions of dna renaturation, about 60 percent of mouse dna fragments renature at a rate consistent with their being present only once per sperm. these nonrepeated sequences (also called single-copy or unique) may be used in rna-dna hybridization experiments to provide quantitative estimates of rna diversity. about 10 percent of the mouse single-copy sequences are transcribed in mouse brain tissue. estimates of about 3 percent were obtained for mouse liver and kidney rna's. if onl ...19714996550
toxic factors in enzymes used in laundry products.autolyzates of bacillus subtilis used as enzymes in laundry products contain several potentially toxic substances.19714996594
[demonstration of a technic of extracting and purifying the peptidoglycan of a gram-negative bacterium]. 19714996651
gamma-ray induced strand breakage of bacillus subtilis dna irradiated in vivo. 19714996652
search for antagonistic actinomycetae in libyan soils. 19714996654
ribosomal rna synthesis is turned off during sporulation of bacillus subtilis. 19714995942
radiosensitization with iodine compounds. 3. macromolecular synthesis and repair in bacillus subtilis irradiated in the presence of iodoacetic acid, potassium iodide or potassium iodate. 19714995962
the action of crude bacterial alpha-amylase on tropocollagen. a case of strictly limited proteolysis. 19714996026
properties and localisation of mycobacillin-synthesising enzyme system in bacillus subtilis b3. 19714996109
the iron-uptake system of bacillus subtilis. 19714994092
the enhancement of immunoprecipitation using antisera from early stages of immunization. 19714994117
antibody production in mice. ii. the mechanism of antigenic stimulation in the secondary immune response. 19714994119
sensitivity to proteolytic enzymes in laundry detergents. 19714994220
production of active phage from b. subtilis cultures. 19714996188
[control of the serum phenylalanine level in the diet therapy of phenylketonuria]. 19714996198
rna synthesis during bacteriophage spo1 development: six classes of spo1 rna. 19714996233
certain aspects of spo1 development. 19714996234
transcription during bacteriophage spo1 development: mutations affecting the program of viral transcription. 19714996235
heterogeneity in nucleotide composition of bacillus subtilis dna. 19714996237
effect of isozanthopterin on the activity of transforming dna in bacillus subtilis. 19714996245
[concentration and separation of bacteria on dowex anionite]. 19714996294
interaction of glutaraldehyde with some micro-organisms. 19714101096
a modified rat mast cell technique for the detection of human ige-type reagins. 19714102848
improved techniques for the preparation of ultrathin frozen sections.ultrathin frozen sections of biological tissues for electron microscopy provide certain advantages in cytochemical studies in which the penetration of cells by large molecules is necessary and in morphological studies of cellular constituents which are dissolved by the reagents employed in routine plastic embedding. the recent introduction of several types of commercially available cryo-ultramicrotomes makes it possible for many laboratories to employ this valuable tool. this paper summarizes re ...19714103954
paracrystalline sheets reaggregated from solubilized exosporium of bacillus cereus.fragments of exosporium, isolated from dormant spores of bacillus cereus, were disintegrated by treatment with sodium dodecyl sulfate (sds) or with phenol and acetic acid. after centrifugation of each preparation, proteins in the supernatant fractions were resolved by disc gel electrophoresis into either two or eight bands, respectively. the sds-solubilized fraction contained spheroidal particles 11 to 44 nm in diameter. when centrifuged until clear, this fraction after dialysis still gave rise ...19714105032
comparison of the biochemistry and rates of synthesis of mesosomal and peripheral membranes in bacillus subtilis.the membrane vesicle (beaded chain) portion of the mesosomes and peripheral (ghost) membrane of bacillus subtilis were obtained by protoplast lysis and separated by differential and sucrose gradient centrifugation. electron microscopy revealed that both fractions were satisfactorily homogeneous. comparison of the two membrane preparations showed that they were similar with respect to total protein, total phosphorus, and lipid-soluble phosphorus content. their protein patterns on acrylamide gel e ...19714105034
the regulation of malate dehydrogenase in sporulation mutants of bacillus subtilis blocked in the citric acid cycle. 19714105893
similarity of bacillus subtilis bacteriophages pbs 1, 3 nt and i10. some remarks on the morphology of phage heads. 19714107335
immunogenic specificity of bacillus subtilis phage spo1-dna. 19714107659
purification and partial characterization of bacillus subtilis flagellar hooks.a method for preparing bacterial flagellar hook structures is described. the method involves isolating intact flagella from a mutant which makes thermally labile flagellar filaments and heat-treating them to disaggregate the filament preferentially. the resulting hook preparation can be separated and purified by velocity and isopycnic centrifugation. the purified hooks sediment at a relative s value of 77. on acrylamide gel electrophoresis in sodium dodecyl sulfate, they show one major and a num ...19714107809
mode of action of zorbamycin.zorbamycin (u-30,604e) induces rapid degradation of deoxyribonucleic acid (dna) and ribonucleic acid (rna) in bacillus subtilis cells. dna degradation is initiated first and is closely followed by the degradation of rna. no interaction between isolated dna and zorbamycin is observed. nucleic acid and protein syntheses are not inhibited by zorbamycin in cell-free systems. since the initial effect of the antibiotic is expressed at the level of the cellular dna fraction, we assume that zorbamycin s ...19714107810
distribution of the sites of alkaline phosphatase(s) activity in vegetative cells of bacillus subtilis.sites of alkaline phosphatase activity have been located by an electron microscopic histochemical (gomori) technique in vegetative cells of a repressible strain sb15 of bacillus subtilis, derepressed and repressed by inorganic phosphate, and in a mutant sb1004 which forms alkaline phosphatase in a medium high in phosphate. the sites of enzyme activity were revealed as discrete, dense, and largely spherical bodies of varying sizes (20 to 150 nm). cells of both repressible and repression-resistant ...19714108474
[a simple semi-quantitative method for the determination of rna-content in microorganisms using pseudoisocyanine]. 19714110594
inactivation of dihydrostreptomycin by staphylococcus aureus. 19714110812
fragmentation of deoxyribonucleic acid by bleomycin. 19714112751
immunological specificity of b. subtilis phage sp01 dna. 19714117643
clinical and laboratory evidence for inactivation of gentamicin by carbenicillin. 19714100015
the practice and application of electron microscope autoradiography. 19714109436
the adjuvant activity of bacillus subtilis and subtilisin. 19714157464
a study of workers exposed to detergent enzymes. 19714253729
sensitivity to enzyme additives in laundry detergent workers. 19714255272
the cell envelope in proteus vulgaris p 18. isolation and characterization of the peptidoglycan component. 19714255900
[binding of penicillins by the blood serum in rabbits with experimental anaphylactic inflammation]. 19714258087
the action of crude alpha-amylase from bacillus subtilis on cartilage glycosaminoglycans. 19714250495
attachment of flagellar basal bodies to the cell envelope: specific attachment to the outer, lipopolysaccharide membrane and the cyoplasmic membrane.a procedure is described for the purification of the escherichia coli outer membrane (lipopolysaccharide or l membrane) with flagella still attached. the resulting lipopolysaccharide membrane was in the form of vesicles that had a trilaminar structure in thin section and contained about 55% lipopolysaccharide and 45% protein. t2 or t4 phage preadsorbed to e. coli were found attached to the purified lipopolysaccharide membrane. flagella were bound to the purified lipopolysaccharide membrane speci ...19714250610
preferential transcription of bacillus subtilis light deoxyribonucleic acid strands during sporulation.messenger ribonucleic acid (rna) from log and sporulation stages of growth were transcribed mainly from the heavy strand of the complementary strands of bacillus subtilis deoxyribonucleic acid (dna). during sporulation a slight transcription shift from heavy to light dna strands was observed. rna-dna hybrid competition experiments revealed that this shift was due to sporulation-specific transcription from light-dna strands.19714324802
induction of citrate transport in bacillus subtilis during the absence of phospholipid synthesis.citrate transport can be induced in a glycerol-requiring mutant of bacillus subtilis even after deprival of glycerol. under these conditions de novo phospholipid synthesis is stopped although ribonucleic acid, deoxyribonucleic acid, and protein syntheses continue. it is concluded that the inducibility of bacterial transport systems must not necessarily require de novo phospholipid synthesis.19714324807
threonine metabolism in a strain of bacillus subtilis enzymic oxidation of 1-aminopropan-2-ol and aminoacetone. 19714334917
the effects of technical chlordane on energy metabolism of bacillus subtilis. 19714337269
the effects of technical chlordane on growth and energy metabolism of streptococcus faecalis and mycobacterium phlei: a comparison with bacillus subtilis. 19714337270
studies on dextrans and destranases. ix. dextrans elaborated by cariogenic organisms. 19714339591
phosphatases: applications of new methods for their separation. 19714326442
mechanisms for the regulation of enzyme synthesis. 19714326638
[polymyxin m synthesized by bacillus polymyxa var. ross under different conditions of cultivation]. 19714326875
glucosylation of teichoic acid: solubilization and partial characterization of the uridine diphosphoglucose: polyglycerolteichoic acid glucosyl transferase from membranes of bacillus subtilis.polyglycerolteichoic acid:glucosyl transferase (tag transferase), one of the three enzymes involved in the pathway leading to the glucosylation of teichoic acid in bacillus subtilis 168, was investigated. during the early stages of the growth of b. subtilis, tag transferase is predominantly a soluble enzyme found in the cytoplasm. as growth proceeds, the amount of soluble enzyme decreases and the proportion of insoluble, membrane-bound tag transferase increases, reaching a maximal value at the c ...19714327509
stimulation of bacitracin production in the mixed cultures of bacillus subtilis and pseudomonas sp. 19714327540
specific assay of aminoglycosidic- or polymyxin-type antibiotics present in human sera in combination with cephalosporins.monitoring of serum concentrations of aminoglycosidic or polymyxin antibiotics when administered concurrently with cephalosporins or penicillins requires a special assay technique. selective enzymatic degradation of the beta-lactam antibiotic from the serum specimen allows subsequent assay of the antibiotic being monitored. this report gives details of a simple procedure for laboratory production of a crude enzyme capable of degrading cephalosporins or penicillins. an assay procedure for quantit ...19714327609
[oxido-reduction reactions at the level of cytoplasmic membranes and mesosomes of bacillus subtilis]. 19714327794
atp: glutamine synthetase adenylytransferase from escherichia coli: purification and properties of a low-molecular weight enzyme form. 19714328161
synthesis and in vitro antimicrobial evaluation of hydrazones of 1-phenyl-, 1-benzyl-, and 1-benzhydryl-4-aminopiperazines. 19714328475
asymmetric template function of microbial deoxyribonucleic acids: transcription of messenger ribonucleic acid.in bacillus subtilis and escherichia coli, pulse-labeled ribonucleic acid (rna) synthesized during step-down growth hybridized preferentially with the heavy (h) strand of methylated albumin-kieselguhr-fractionated deoxyribonucleic acid (dna). at high rna inputs, the ratio of rna hybridized with the h strand to that hybridized with the light (l) strand was 8.7 for b. subtilis and 2.0 for e. coli. at high dna inputs, the h/l hybridization ratio increased by a factor of two. this change in the hybr ...19714328752
reactivative action of ethylenediaminetetraacetic acid or dipicolinic acid on inactive glucose dehydrogenase obtained from heated spores of bacillus subtilis.partially purified inactive glucose dehydrogenase obtained from spores which were heated at 87 or 90 c for 30 min is converted to an active from by the addition of ethylenediaminetetraacetic acid, dipicolinic acid, or some salts. the molecular weight of the inactive glucose dehydrogenase in the heated spores is about one-half of that of the active glucose dehydrogenase in the intact resting spores. the possibility is discussed that the active glucose dehydrogenase in the intact resting spores di ...19714329730
catalytic hydrogenation of viomycin and capreomycin. 19714329900
ribosomal ribonucleic acid synthesis in bacillus subtilis.the mode of biosynthesis of the 16s and 23s ribosomal ribonucleic acids (rrna) was studied in bacillus subtilis 168thy(-). three criteria were used to define the characteristics of the rrna species: (i) the time required at 37 degrees c to synthesize 16s and 23s rrna chains de novo in growing cultures; (ii) the degree of reactivity of the 3'-terminal groups of the rrna molecules with periodate and [carbonyl-(14)c]isonicotinic acid hydrazide; and (iii) the reactivity of the 5'-terminal regions of ...19714330148
primary sequence of trna val from escherichia coli b. i. oligonucleotide sequences of digests of escherichia coli trna val with rnase t and pancreatic rnase. 19714330328
structure and synthesis of a lipid-containing bacteriophage. v. phospholipids of the host bal-31 and of the bacteriophage pm2. 19714330370
altered deoxyribonucleic acid polymerase activity in a methyl methanesulfonate-sensitive mutant of bacillus subtilis.of six deoxyribonucleic acid repair mutants of bacillus subtilis assayed for deoxyribonucleic acid polymerase, only the methyl methanesulfonate-sensitive and ultraviolet light-sensitive mutant jb1-49(59) has impaired polymerase activity. extracts prepared by sonic treatment or gentle lysis had about 10% of the wild-type activity with poly d(a-t), an alternating copolymer of deoxyadenylate and deoxythymidylate, used as template. the sensitivity to methyl methanesulfonate and ultraviolet light and ...19714330738
inactivation of d-alanine carboxypeptidase by penicillins and cephalosporins is not lethal in bacillus subtilis.the d-alanine carboxypeptidase of bacillus subtilis is a particulate enzyme that is irreversibly inactivated by penicillins and cephalosporins. however, the lethal concentrations of these antibiotics are not the same as those that inhibit enzymatic activity in vitro. 6-aminopenicillanic acid inactivates at least 95% of the enzyme at nonlethal concentrations. conversely, cephalothin is lethal at concentrations that do not inactivate the enzyme. experiments with intact, growing cells confirm the r ...19714330943
mechanisms of active transport in isolated membrane vesicles. 2. the coupling of reduced phenazine methosulfate to the concentrative uptake of beta-galactosides and amino acids. 19714331061
distribution of ampicillin administered orally in three different forms in rabbit. 19714331145
electron microscopic study of the secretion of some bacterial enzymes and toxins. 19714331146
phenotypic reversion in some early blocked sporulation mutants of bacillus subtilis. genetic study of polymyxin resistant partial revertants. 19714331231
phenotypic reversion in some early blocked sporulation mutants of bacillus subtilis: isolation and phenotype identification of partial revertants.single-step mutants of bacillus subtilis blocked at stage zero of sporulation (spoo mutants) are pleiotropic. they are impaired in several traits, including increased sensitivity to polymyxin b, when compared to the wild type. revertants for one or another of the traits associated in this pleiotropy have been isolated and classified according to their phenotypes. attention was centered on partial revertants (spoo still), of which at least three classes could be recognized. class i partial revert ...19714331293
changes in terminal respiratory pathways of bacillus subtilis during germination, outgrowth and vegetative growth.the chemical and enzymatic properties of the cytochrome system in the particulate preparations obtained from dormant spores, germinated spores, young vegetative cells, and vegetative cells of bacillus subtilis pci219 were investigated. difference spectra of particulate fractions from dormant spores of this strain suggested the presence of cytochromes a, a(3), b, c(+c(1)), and o. all of the cytochrome components were present in dormant spores and in germinated spores and vegetative cells at all s ...19714331498
structure and biological activity of deoxyribonucleic acid from bacillus bacteriophage phi 105: effects of escherichia coli exonucleases.the effects of escherichia coli exonuclease i, exonuclease iii, and deoxyribonucleic acid (dna) polymerase on the biological activity of mature dna from temperate bacillus bacteriophage phi105 were investigated. intact dna loses infectivity rapidly upon exposure to exonuclease iii. although there is an overall decrease in marker rescue from exonuclease iii-digested dna, digestion preferentially affects markers at the end of the genetic map. this is taken to indicate a nonpermuted gene sequence i ...19714323711
dna polymerase activity in homogenates of cells infected with mc29 virus. 19714323849
microbial lipoamide dehydrogenase. purification and some characteristics of the enzyme derived from selected microorganisms. 19714323856
enzymatic basis of metabolic stimulation in leucocytes during phagocytosis: the role of activated nadph oxidase. 19714399354
acrylamide gel electrophoresis of intracellular proteins during early stages of sporulation in bacillus subtilis.acrylamide gel electrophoresis of unfractionated cellular extracts of bacillus subtilis is shown to be an effective method for characterizing many of the changes in protein composition, when coupled with specific histological-type staining reactions. the results obtained here by using extracts from cells at different stages of growth and sporulation are consistent with observations from other laboratories where extensively purified and highly characterized enzymes have been studied. in several i ...19714399643
quantitative studies on the salivary flora.in a quantitative bacteriological study of the salivary flora from 50 children the following aerobic organisms were identified and enumerated: alpha-haemolytic streptococci, beta-haemolytic streptococci, streptococcus faecalis, pneumococci, staphylococcus aureus, staph. albus and staph. citreus, neisseria spp, n. meningitidis, corynebacteria, aerobic lactobacilli, b. subtilis, h. influenzae, coliform organisms, and candida spp. many of the known potentially pathogenic members were present in lar ...19714399776
l-threonine acetaldehyde-lyase in a strain of bacillus subtilis. 19714401030
the effect of temperature on the survival of microorganisms in a deep space vacuum. 19714401390
how penicillin kills bacteria: progress and problems. 19714401416
fate of bacillus subtilis transforming deoxyribonucleic acid incorporated into transformable diplococcus pneumoniae. 19714395925
the biochemical and antimicrobial activities of phagocytizing cells. 19714395983
[bacteriostatic effect of tetracyclines-macrolides associations]. 19714397549
nadph-regenerating systems in microorganisms producing macrolide antibiotics. 19714397940
preparation and properties of a solubilized form of cytochrome p-450 from chick embryo liver microsomes. 19714398361
nicotinamide dinucleotides in microorganisms producing peptide and macrolide antibiotics. 19714398752
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