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convulsions in childhood shigellosis. clinical and laboratory features in 153 children.we studied 153 children who experienced convulsions associated with shigellosis. the male-female ratio was 1.2:1.0. thirty-six children had a previous history of febrile convulsions, and 31 children had a family history of convulsive disorder. most of the children were 0.5 to 3 years of age, although 49 (32%) were older than 3 years of age and 20 (13.1%) were older than 5 years of age. all children were febrile; in 75% of the children, the temperature was over 39 degrees c. the majority of the c ...19873544808
plasmid-mediated early killing of eucaryotic cells by shigella flexneri as studied by infection of j774 macrophages.in shigella flexneri a 220-kilobase plasmid encodes the ability to invade nonprofessional phagocytes by a mechanism similar to phagocytosis. in this report, the continuous macrophage cell line j774 was used to study the intracellular fate of both invasive and noninvasive strains. pwr100, the virulence plasmid of s. flexneri serotype 5, mediated very efficient and rapid killing of j774 macrophages, as measured by cellular detachment and uptake of trypan blue. for this to occur, the bacteria had t ...19873546130
the relevance of controlled fluoride release with bonded orthodontic appliances. 19873323277
[dependence of the antimicrobial effect on the antibiotic concentration in modelling pharmacokinetic profiles in vitro: a comparison of 2 regimens for the single intravenous administration of sisomicin].kinetics of sisomicin antimicrobial effect on e. coli was studied in an in vitro dynamic model. pharmacokinetic profiles observed in blood of humans treated with different polar dosing schedules i. e. bolus administration and continuous infusion were simulated. the first set of the experiments included simulating the profiles observed at short-term maintenance (0.25-20 min) of relatively high antibiotic levels after bolus administration (cmax = 0.125-10 micrograms/ml). the second set included si ...19873324953
[dependence of the antimicrobial effect on the antibiotic concentration in modelling pharmacokinetic profiles in vitro: a comparison of 2 regimens for the single intravenous administration of sisomicin].kinetics of sisomicin antimicrobial effect on e. coli was studied in an in vitro dynamic model. pharmacokinetic profiles observed in blood of humans treated with different polar dosing schedules i. e. bolus administration and continuous infusion were simulated. the first set of the experiments included simulating the profiles observed at short-term maintenance (0.25-20 min) of relatively high antibiotic levels after bolus administration (cmax = 0.125-10 micrograms/ml). the second set included si ...19873324953
a new keratinolytic proteinase from clinical isolates of trichophyton mentagrophytes. 19873325542
[economic loss from dysentery combined with ascariasis and trichocephaliasis]. 19873325790
ascending urinary tract infections in rats induced by staphylococcus saprophyticus and proteus mirabilis.ascending pyelonephritis was induced by staphylococcus saprophyticus and proteus mirabilis both by inoculating the organisms separately and in combination into rat bladders. bacterial cultures of tissue homogenates showed that pyelonephritis by both bacteria occurred significantly more often in rats where the two organisms were instilled concomitantly, suggesting a synergistic virulence between the two species. the antibody response to the different organisms was the same either the bacteria wer ...19873326399
effect of outer membrane proteins of shigella on delayed hypersensitivity in mice to sheep red blood cells.small doses of outer membrane proteins (omp) of shigella flexneri injected intraperitoneally into mice 1 to 3 days before or 3 days after sensitization of animals with sheep erythrocytes were found to increase delayed hypersensitivity as measured by the footpad reaction. in contrast, administration of higher doses of omp resulted in suppression of hypersensitivity response. cell transfer experiments showed that the spleen cells from sensitized and omp treated mice transferred stimulating and sup ...19873326545
multiple drug-resistance in shigella flexneri isolated from a patient with human immunodeficiency virus.persistent shigellosis, due to shigella flexneri resistant to multiple antibiotics, developed in a 40-yr-old homosexual man with human immunodeficiency virus infection. the shigella strain demonstrated resistance to ampicillin, tetracycline, and trimethoprim-sulfamethoxazole. although shigella flexneri isolates resistant to trimethoprim-sulfamethoxazole are uncommon in the united states, laboratories should monitor resistance patterns through routine in vitro susceptibility testing.19873326711
phylogeny of lymphocyte heterogeneity: identification and separation of functionally distinct subpopulations of channel catfish lymphocytes with monoclonal antibodies.the purpose of this study was to identify monoclonal antibodies reactive with channel catfish t cells. since a variety of commercially available anti-human and mouse t cell reagents failed to react with channel catfish leucocytes, the development of anti-fish t cell monoclonal antibodies was undertaken. one of these reagents, designated mab 13c10, reacted with channel catfish surface immunoglobulin negative, but not positive, lymphocytes. this reagent, when used in "panning" protocols, was able ...19873326758
differential clinical features and stool findings in shigellosis and amoebic dysentery.to obtain information that could assist the clinician to differentiate between shigellosis and amoebic dysentery, we compared clinical features and stool findings in 58 adult male patients in bangladesh. mean values indicated that patients with invasive amoebiasis were older and had a longer prehospital illness, a lower body weight, less frequent fever, a lower haematocrit and a higher white blood cell count than patients with shigellosis. the mean number of faecal leucocytes per mm3 was signifi ...19873328339
[heterogeneity of shigella flexneri during selective synchronization].the study of a number of main growth characteristics of s. flexneri (s) cells during the first and second cycles of their cultivation in a solid culture medium in peshkov's chamber from seed material, nonsynchronized and synchronized by c. e. helmstetter's method, has revealed that this method does not permit obtaining completely synchronized cultures. four physiological cell clusters, differing in the time of generation, growth rate, size at the beginning and the end of the cycle and the time o ...19873328453
[campylobacter jejuni in patients with diarrhea].in this study the stools 125 patients with diarrhea applied to research hospital of medical school of atatürk university and the stools of 69 healthy persons were investigated for gastroenteritis agents c. jejuni as well as salmonella and shigella. of the zooparasiter agents protozoon and helminths were also investigated. we determined the mentioned agents in 28 of 125 patients (% 22.40). c. jejuni was isolated the most frequent cause of diarrhea with 8.80 percent. c. jejuni was isolated in high ...19873328817
[construction of a typhoid-dysentery bivalent strain with typhoid ty21a and dysentery t32-istrati strains]. 19873329424
[biochemical aspects of an endotoxin model for endogenous uveitis]. 19873329451
a controlled trial of nebulized aminoglycoside and oral flucloxacillin versus placebo in the outpatient management of children with cystic fibrosis.six children with cystic fibrosis who had persistently had pseudomonas aeruginosa isolated from their respiratory tract, completed a double-blind cross-over comparison of oral flucloxacillin and nebulized aminoglycoside versus double placebo. the patients had higher fev1 results at the end of the month of active treatment than after the month of placebo.19873329531
[trial use of embryonal and early-fetal thymus extracts in the treatment of non-healing skin defects. ii. crural ulcers in humans]. 19873329747
xylose lysine desoxycholate agar for recovering shigella dysenteriae 1 and shigella flexneri from faeces. 19873330107
on a survey of plasmid dna in the clinically isolated shigella strains in shanghai area and comparison with those from south east asia. 19873331655
a retrospective analysis of the occurrence of shigella flexneri serotypes in some african countries. 19873333153
outbreak of dysentery due to shigella flexneri type 2a. 19873333777
erg, a human ets-related gene on chromosome 21: alternative splicing, polyadenylation, and translation.the avian acute leukemia virus e26 induces a mixed erythroid-myeloid leukemia in chickens and carries two distinct oncogenes, v-myb and v-ets. recently, a novel gene named erg, closely related to the v-ets oncogene, was identified in human colo 320 cells and the nucleotide sequence of its approximately 5.0-kilobase transcript, erg 1 was determined. in the present study, the nucleotide sequence of the alternatively spliced transcript, erg 2, was found to differ from erg 1 by a splicing event that ...19873299708
[growth and development of a population of the causative agent during an infection]. 19873299739
multiple enteric pathogens in a patient with diarrhoea.a woman who recently arrived in canada from india developed a febrile, diarrhoeal illness. stool culture revealed the presence of salmonella typhi, campylobacter fetus subspecies jejuni and shigella flexneri. concurrent infection with these three pathogens does not appear to have been previously reported. the possibility of multiple pathogens should be considered in travellers who fail to respond to treatment of a single organism.19873299934
corneal ulcer caused by shigella flexneri in an infant. 19873300355
epinephrine, glucose, and lactate infusion in exercising adrenodemedullated rats.the purpose of this study was to determine the metabolic function of the marked increase in plasma epinephrine which occurs in fasted rats during treadmill exercise. fasted adrenodemedullated (adm) and sham-operated (sham) rats were run on a rodent treadmill (21 m/min, 15% grade) for 30 min or until exhaustion. adm rats were infused with saline, epinephrine, glucose, or lactate during the exercise bouts. adm saline-infused rats showed markedly reduced endurance, hypoglycemia, elevated plasma ins ...19873298192
effects of marine mammal parasites on human health. 19873294653
molecular cloning of invasion plasmid antigen (ipa) genes from shigella flexneri: analysis of ipa gene products and genetic mapping.tn5-tagged invasion plasmid dna (pwr110) from shigella flexneri serotype 5 (strain m90t) was cloned into the expression vector lambda gt11. recombinant phage (lambda gt11sfl) expressing pwr110-encoded polypeptide antigens were identified by using rabbit antisera directed against s. flexneri m90t invasion plasmid antigens. antigens encoded by lambda gt11sfl recombinant phage were characterized by reacting affinity-purified antibodies, eluted from nitrocellulose-bound plaques of lambda gt11sfl rec ...19873294797
an aminoacyl-trna synthetase complex in escherichia coli.aminoacyl-trna synthetases from several strains of escherichia coli are shown to elute as a high-molecular-weight complex on 6% agarose columns (bio-gel a-5m). in contrast, very little synthetase activity was observed in such complexes on sephadex g-200 columns, suggesting that these enzymes may interact with or are dissociated during chromatography on dextran. the size of the complex observed on bio-gel a-5m was influenced by the method of cell breakage and the salt concentrations present in bu ...19873294804
an accessory protein identical to mouse egasyn is complexed with rat microsomal beta-glucuronidase and is identical to rat esterase-3.we report biochemical, immunological, and genetic studies which demonstrate that an accessory protein with the essential features of mouse egasyn is complexed with and stabilizes a portion of beta-glucuronidase in microsomes of rat liver. the accessory protein exists as a complex with beta-glucuronidase since it coprecipitates with beta-glucuronidase after treatment of extracts with a specific beta-glucuronidase antibody. the two proteins are associated by noncovalent bonds since they are easily ...19873294829
bacterial invasion of fibroblasts induces interferon production.although bacteria and/or bacterial products have been shown to induce interferon (ifn) production in cells of the immune system, the ability of non-lymphoid cells to produce ifn in response to bacterial products and/or bacterial invasion is currently unknown. in this study we examined murine and human fibroblasts for their ability to produce ifn in response to challenge with the invasive bacteria shigella flexneri and enteroinvasive escherichia coli. both human and murine primary cell cultures p ...19873295042
toxic megacolon complicating shigellosis. 19873295219
[dynamics of bacterial polysensitization in acute dysentery in children depending on the species of the causative agent].studies on polysensitization, evaluated by the neutrophil damage index, have been made with the use of dysenterin and the allergens of hemolytic staphylococci, enterococci and escherichia coli. the studies have revealed that both specific and nonspecific sensitization to hemolytic staphylococci and enterococci is higher in dysentery caused by shigella flexneri than in dysentery caused by s. sonnei and has the tendency to increase in the course of the disease.19873296580
[gardnerella vaginalis in nonspecific vaginitis]. 19873296818
[reye-like syndrome due to shigella flexneri in a child]. 19873301584
treatment of bacillary dysentery: a comparison between enoxacin and nalidixic acid.a prospective randomized study has been conducted in rwanda to compare the efficacy of enoxacin versus nalidixic acid for the treatment of bacillary dysentery in adults. seventeen patients received nalidixic acid and 21 received enoxacin. all isolates (37 shigella, 1 campylobacter) were sensitive in vitro to the two drugs. all patients showed a favourable clinical and bacteriological response and the results obtained with both drugs were comparable. enoxacin seems to be a good alternative to nal ...19873301783
clinical presentation of shigellosis during the 1984 epidemic of bacillary dysentery in west bengal. 19873301795
shigellosis in bangladesh: a reply. 19873302055
retardation of cell cycle progression in yeast cells recovering from dna damage: a study at the single cell level.pedigree analyses of individual yeast cells recovering from dna damage were performed and time intervals between morphological landmark events during the cell cycle (bud emergence and cell separation), were recorded for three generations. the associated nuclear behavior was monitored with the aid of dapi staining. the following observations were made: all agents tested (x-rays, mms, ems, mnng, nitrous acid) delayed the first bud emergence after treatment, which indicates inhibition of the initia ...19873302599
production of monospecific antibodies to a low-abundance hepatic membrane protein using nitrocellulose immobilized protein as antigen.membrane proteins from primary cultures of rat hepatocytes were separated by two-dimensional polyacrylamide gel electrophoresis. the proteins were transferred to nitrocellulose paper which was then dissolved in dimethyl sulfoxide and this mixture was used as a primary immunogen in rabbits. subsequent immunizations were performed using nonsolubilized protein immobilized on nitrocellulose paper. a monospecific polyclonal antibody was generated against a specific mitochondrial membrane protein (mp- ...19873304007
molecular cloning and expression of a synthetic dna coding for the antimicrobial protein of bull seminal plasma.a dna carrying the coding sequence for the antimicrobial protein from bull seminal plasma (sap) was obtained by enzymic ligation of six synthetic oligonucleotides. the 162 bp synthetic dna fragment was cloned into the c-terminal part of the lacz-gene employing the vector pur289. expression in e. coli in the presence of the inducer isopropylthiogalactoside (iptg) led to the formation of a fusion protein, which was shown by immuno-blotting to contain immuno-reactive antimicrobial protein. approxim ...19873304335
[reaction of the hematopoietic system in mice to the administration of virulent and avirulent strains of shigella flexneri 2a].the influence of live bacteria and antigenic preparations of virulent and avirulent stains of shigella flexneri 2a on the endogenous splenic colony formation in murine hematopoietic cells has been studied. the different stimulating activity of live microbial cells of virulent and avirulent strains shigella flexneri 2a and their antigenic preparations on the endogenous colony formation has been shown. the effect observed depends on the preparation doses and time of their administration before irr ...19873304434
a new chromogenic test for the detection of urokinase in the genus shigella.a simple test for detection of urokinase in shigella is described. the test is performed by suspending a loopful of bacteria in 100 microliters of a buffered 1mm solution of benzoyl-beta-alanyl glycyl-arginyl-4-nitroanilide acetate (chromozym u). enzymatic activity is revealed by formation of a yellow colour after 24 h of incubation at 37 degrees c. the test is able to differentiate serotypes of shigella dysenteriae, shigella flexneri, shigella boydii. the results suggest the possibility of incl ...19873306294
chronobiology and epidemiology.epidemiology and chronobiology are two emerging scientific disciplines of the 20th century. in the united states and certain european and asian countries, epidemiology is a well-developed science; however, in many countries the field is not yet well practiced. too, in several european countries and certain centers in the united states, chronobiology is quite well known and respected as a science. yet, in many countries this field is just now being recognized. although epidemiologists are concern ...19873306581
[morphological changes in the lungs after single whole-body irradiation in rabbits].morphological changes in rabbit lungs were studied after whole-body nonuniform irradiation (24 gy) the head and the abdomen being shielded. acute pneumonitis was shown to develop between the 17th and the 24th day after irradiation. no pulmonary fibrosis was registered 60 days following irradiation. embolised megakaryocytes were found in the lung capillaries between the 8th and the 24th day after irradiation.19873306764
atrio-ventricular block associated with shigella flexneri infection. 19873311739
entry of shigella flexneri into hela cells: evidence for directed phagocytosis involving actin polymerization and myosin accumulation.the enteroinvasive bacterium shigella flexneri expresses a plasmid-mediated capacity to penetrate into nonphagocytic cells. by using 7-nitrobenz-2-oxa-1,3-diazole-phallacidin (nbd-phallacidin), a fluorescent dye which specifically stains microfilaments, we observed condensations of filamentous actin underneath the plasma membrane of hela cells which interacted with the invasive isolate m90t. as demonstrated by indirect immunofluorescence with the antimyosin monoclonal antibody cc-212, myosin acc ...19873312007
introduction of shigella flexneri 2a type and group antigen genes into oral typhoid vaccine strain salmonella typhi ty21a.for protection against dysentery caused by shigella flexneri 2a, an in vivo-constructed recombinant plasmid with genes specifying the s. flexneri type and group antigens located near the pro (min 6) and his (min 44) chromosomal markers, respectively, was made and transferred to the gale salmonella typhi strain ty21a. strain ty21a carrying this recombinant plasmid was shown by immunological and biochemical analyses to express the s. flexneri 2a type and group antigens. mice immunized with this va ...19873312013
expression of campylobacter jejuni invasiveness in cell cultures coinfected with other bacteria.enteroinvasive salmonella, shigella, and escherichia coli strains were found to exert an effect which rendered campylobacter jejuni capable of intracellular localization in epithelial cells in vitro. when monolayers of hep-2 or a-549 cells were challenged with pure cultures of c. jejuni or campylobacter coli, none of the eight strains tested invaded the cells. in contrast, four of these strains were able to localize intracellularly when the cells were challenged with a mixture of campylobacters ...19873312015
[bacillary dysentery 1985]. 19873313536
hemolytic uremic syndrome associated with shigellosis: report of two cases.two patients with hemolytic uremic syndrome admitted to udornthani hospital, thailand, were reported. the preceding illness was shigellosis. the stool culture was positive for shigella dysenteriae in one patient and shigella flexneri in another. the management was successful with peritoneal dialysis in one and symptomatic treatment in another. both patients had complete recovery.19873313740
[prospects for using thymalin in treating bacterial intestinal infections]. 19873314133
technological investigations with attenuated strains of shigella for production of live vaccines. 1. cultivation in a fermenter of an attenuated strain s. flexneri 2a with two markers.a method was developed for cultivation of the strain shigella flexneri 2a 77 with two attenuated markers. it ensures the preservation of the initial properties of the strain. the regimen for control of dissolved oxygen level was optimized. it was established that yeast extract is a necessary component of the nutritive medium used. the role of the phase and quantity of the inoculum for growth of the bacterial population has been clarified. fresh fermenter culture, applied in rats, shows a high im ...19873314220
shigella bacteremia in adults. a report of five cases and review of the literature.shigella bacteremia is rare, occurring mainly in children. we describe five adult patients with shigella bacteremia and review data on 22 cases reported in the english-language medical literature. eighteen (67%) of 27 patients had either an underlying condition or were aged older than 65 years. most patients had clinical signs of acute febrile gastroenteritis. however, in six patients, the organism was not isolated from stool. species isolated from blood included shigella flexneri in 11 patients ...19873314766
a 2d-1h-n.m.r. study of some shigella flexneri o-polysaccharides.a 1h-n.m.r. study of the o-polysaccharides from different types of shigella flexneri has been performed. with the aid of 2d-n.m.r. techniques, namely, j-resolved, spin-spin correlation, and noesy experiments, most of the structural features of these polysaccharides could be deduced. sequences could generally be obtained from the noesy experiments. when using a prolonged mixing time in these experiments, cross-peaks due to spin diffusion from one anomeric proton to the anomeric proton of an adjac ...19873315202
[biological properties of opportunistic microorganisms isolated from patients with acute intestinal diseases].the comparative study of the incidence of the pathogenicity markers (dnaase, rnaase, phosphatase and hemolytic activity) in shigellae and salmonellae, acknowledged as the causative agents of intestinal infections, and in opportunistic bacteria isolated from the feces of patients with acute intestinal diseases and healthy persons has been made. the study has revealed that dnaase and rnaase activity occurs most frequently in shigella flexneri, in salmonellae and in opportunistic enterobacteria iso ...19873318225
shigellosis at a caribbean resort. hamburger and north american origin as risk factors.during a three-week period in 1984, 339 tourists and expatriate employees with diarrhea visited the infirmary at a caribbean resort club. epidemiologic studies suggested that over 60% of the 1,893 tourists at the resort club during that time may have been ill. shigella flexneri 4a was isolated from seven of 18 stool specimens. a few cases of diarrheal illness occurred at the resort club before the onset of the outbreak, which was temporally related to illness in a butcher. north american residen ...19873318410
[medico-genetic studies of human provisional organs]. 19873318529
improved immunohistochemical staining of angiotensin ii in rat brain using affinity purified antibodies.recent immunohistochemical studies that have sought to detect angiotensin ii/iii (aii/aiii) immunoreactive material in the brain have been forced to rely on a small number of antisera because most aii/aiii antibodies have unexplainably proved unsuitable for immunohistochemistry. although extremely useful tools, these antisera have suffered from high background staining. the purpose of this study was to re-examine and characterize the staining using the most popular aii/aiii antiserum (denise) be ...19873319044
pathogenesis of shigella infections. 19873321018
comparative aspects of canine and human atopic dermatitis. 19873321301
novel oral vaccines: the effectiveness of cloned gene products on inducing secretory immune responses. 19873321939
inhibition of enteropathogenic escherichia coli (strain rdec-1) adherence to rabbit intestinal brush borders by milk immune secretory immunoglobulin a. 19873321942
enhancement of the secretory iga response to enteropathogens. 19873321943
virulence of iron transport mutants of shigella flexneri and utilization of host iron compounds.mutants of shigella flexneri defective in aerobactin-mediated iron transport were assayed for virulence in several model systems. a tn5 insertion mutant was invasive in hela cells, lethal in the chicken embryo, and produced keratoconjunctivitis in the guinea pig, indicating little or no loss of ability to invade and multiply intracellularly. although the mutant failed to grow in low-iron medium in vitro, growth equivalent to that of the wild type was observed in hela cell lysates. thus, there ap ...19873028962
porous hydroxyapatite as a bone graft substitute in diaphyseal defects: a histometric study.porous hydroxyapatite (ip200), formed by conversion of the poritidae porites exoskeleton, has pores averaging 230 microns and pore interconnections averaging 190 microns in diameter. in the distal radial diaphyses of 14 dogs, bilateral 7.5 x 20 mm cortical windows were created and fitted with 5 x 7.5 x 20 mm blocks of ip200 implants and iliac autografts. both implanted and contralateral grafted radius specimens were retrieved at 3, 6, 12, 24, and 48 months. unstained undecalcified sections were ...19873029358
on the fidelity of dna replication: herpes dna polymerase and its associated exonuclease.procaryotic dna polymerases contain an associated 3'----5' exonuclease activity which provides a proofreading function and contributes substantially to replication fidelity. dna polymerases of the eucaryotic herpes-type viruses contain similar associated exonuclease activities. we have investigated the fidelity of polymerases purified from wild type herpes simplex virus, as well as from mutator and antimutator strains. on synthetic templates, the herpes enzymes show greater relative exonuclease ...19873029700
a cellular protein binds to a conserved sequence in the adenovirus type 2 enhancer.a sensitive gel retention assay has been utilized to detect proteins from uninfected hela nuclei which interact with the adenovirus type 2 enhancer. this assay has been employed to monitor fractionation of nuclear extracts. three enhancer binding factors were resolved by chromatography on deae-sepharose and one of the factors was further purified by chromatography on heparin-sepharose. dnase protection experiments have shown that the heparin-sepharose fraction contains a factor which binds predo ...19873031610
integration and expression of provirus in human cells transformed by avian sarcoma virus.previously, human diploid fibroblasts from some donors infected in vitro by avian sarcoma virus (asv) were transformed and found, by electron microscopy, to produce small numbers of virus particles that were infectious by bioassay; also, a line of human osteosarcoma cells infected with asv developed additional characteristics of transformation and released a small number of infectious virus particles. in this study the complete proviral sequence was shown to be integrated in the genome of these ...19873033382
effects of inhibitors of the cytoplasmic structures and functions on the early phase of infection of cultured cells with simian virus 40.to obtain information about cytoplasmic structures and functions involving the entry of simian virus 40 virions into cells, we examined whether the inhibitors that affect the functions and/or structure of lysosomes, cell membrane, and cytoskeletons inhibit expression of nuclear t antigen in the sv40-inoculated rat 3y1 and monkey cv-1 cells. chloroquine, methylamine, and butylamine did not inhibit t-antigen expression, suggesting that lysosomal acidification is not required for establishment of i ...19873033894
the effect of cycloleucine on sfv a7(74) infection in mice.cycloleucine (cl), a non-metabolizable amino acid analogue, was found to reduce thymus and spleen weights in semliki forest virus (sfv) strain a7(74) infected and control mice. the maximum effects were seen when three daily doses of cl were given to mice 24 h after an sfv a7(74) infection. in these mice thymus atrophy led to abolition of thymus dependent immune responses and changes in the pathological features of the viral infection--the most striking feature being prevention of demyelination. ...19873034321
chronic food restriction and the circadian rhythms of pituitary-adrenal hormones, growth hormone and thyroid-stimulating hormone.adult male sprague-dawley rats were subjected to food restriction so that they ate 65% of food ingested by control rats. while control rats had free access to food over the 24-hour period, food-restricted rats were provided with food daily at 10 a.m. the experimental period lasted for 34 days. on day 35, rats from both experimental groups were killed at 08.00, 11.00, 14.00, 24.00 and 02.00 h. food restriction modified the circadian rhythms of acth and corticosterone. in addition, total circulati ...19873035995
chronic food restriction and the circadian rhythms of pituitary-adrenal hormones, growth hormone and thyroid-stimulating hormone.adult male sprague-dawley rats were subjected to food restriction so that they ate 65% of food ingested by control rats. while control rats had free access to food over the 24-hour period, food-restricted rats were provided with food daily at 10 a.m. the experimental period lasted for 34 days. on day 35, rats from both experimental groups were killed at 08.00, 11.00, 14.00, 24.00 and 02.00 h. food restriction modified the circadian rhythms of acth and corticosterone. in addition, total circulati ...19873035995
[expressing plasmid vectors on the basis of escherichia coli beta-galactosidase gene fragments].with the use of synthetic dna fragments, a set of new plasmid vectors has been obtained. the vectors provided high level expression of peptides and small proteins in e. coli as fusions with fragments of beta-galactosidase of various length. these vectors were used to achieve expression of a synthetic gene for a functionally active fragment of bacteriorhodopsin. the yields of hybrid proteins consisting of beta-galactosidase and bacteriorhodopsin fragments were in the range of 5-30% from the total ...19873036168
a high-yield, high-purity elutriation method for preparing human granulocytes demonstrating enhanced experimental lifetimes.a counterflow centrifugal elutriation procedure for preparing human granulocytes is described. this method permits isolation of 1-2 x 10(9) granulocytes with a yield of 70% +/- 7% from a unit of whole blood in 3 h. these cells are 99% +/- 1% pure and 95-99% viable. when compared to cells prepared by a conventional procedure employing hypotonic lysis to remove red cells, the elutriated cells show enhanced oxidative responses resulting from enhanced longevity. other responses including receptor-me ...19873036986
structure of the 3' region of the human elastin gene: great abundance of alu repetitive sequences and few coding sequences.two overlapping clones encompassing 8.5 kb of the human elastin gene were isolated from two genomic libraries constructed by partial digestion with either haeiii/alui or sau3a and contained in lambda charon 4a or embl3, respectively. the 6 kb of dna comprising the most 3' portion of the gene were sequenced demonstrating an extremely low coding ratio since only three exons containing a total of 134 translated nucleotides were identified. two exons totaling 78 bp of translated sequences which were ...19873038460
structure of the 3' region of the human elastin gene: great abundance of alu repetitive sequences and few coding sequences.two overlapping clones encompassing 8.5 kb of the human elastin gene were isolated from two genomic libraries constructed by partial digestion with either haeiii/alui or sau3a and contained in lambda charon 4a or embl3, respectively. the 6 kb of dna comprising the most 3' portion of the gene were sequenced demonstrating an extremely low coding ratio since only three exons containing a total of 134 translated nucleotides were identified. two exons totaling 78 bp of translated sequences which were ...19873038460
complete amino acid sequence of cytochrome c551 from erythrobacter species strain och 114.the complete amino acid sequence of cytochrome c551 isolated from an aerobic photosynthetic bacterium, erythrobacter sp. strain och 114, was determined. the cytochrome molecule was composed of a total of 119 amino acid residues and its molecular weight including heme was calculated to be 13,235. the sequence was (sequence: see text). its molecular weight indicates that this cytochrome is of the l-type. sequence alignment with other bacterial cytochromes c shows that this cytochrome is similar to ...19873038859
[functional macrophage activity in infection with virulent and avirulent strains of the dysentery microbe].the effect of s. flexneri virulent and avirulent (vaccine) strains 2a on the cytoplasmic membrane of mouse macrophages has been studied by evaluating the action of these bacteria on the activity of 5-nucleotidase. the dynamics of the activity of 5-nucleotidase after the introduction of both virulent and avirulent strains has a phasic character with alternating rises and falls in the activity of this enzyme in comparison with the control. s. flexneri vaccine strain produces mainly a stimulating e ...19873039764
evaluation with an iuc::tn10 mutant of the role of aerobactin production in the virulence of shigella flexneri.to evaluate the role of aerobactin production in the virulence of shigella flexneri, a iuc::tn10 insertion mutant was obtained from strain m90t, a serotype 5 isolate. this mutant was tested for its ability to invade and kill hela cells in monolayers, to elicit keratoconjunctivitis in guinea pigs and to infect ligated segments of rabbit ileal loops. although this mutant did not grow in iron-depleted media, its ability to grow intracellulalry and eventually kill hela cells was unchanged from that ...19873040587
neurosecretion of human hypothalamic immunoreactive beta-endorphin: in vitro regulation by dopamine.an in vitro perifusion system was used to investigate immunoreactive beta-endorphin (beta-end-i) release from adult human hypothalami in response to dopamine (da) and the da receptor antagonist haloperidol (hal). administration of a 1 microm pulse of da consistently elicited a mean (+/- se) 88 +/- 9% increase (p less than 0.05, n = 5) in beta-end-i release, whereas 1 microm hal had no effect. administration of 1 microm da during three perifusions in which 1 microm hal was added to the medium fai ...19872951610
reversible harmless interruption of testicular blood supply in the ram.an effective method of interrupting testicular blood flow temporarily and repeatedly in the ram has been developed. blockade of flow has been achieved mechanically by an inflatable occluder placed around the testicular artery at the level of the spermatic cord. the effect of the blockade on total testicular blood supply was investigated using doppler flowmetry and a percutaneous xenon-133 injection method. with both approaches, the blood flow changes after inflation or deflation of the occluders ...19872953703
[a case of severe colitis with positive hemocultures for shigella flexneri]. 19872954108
balloon-dilatable arterial banding prosthesis: experimental study.two arterial banding prostheses were developed that could be dilated by an intraluminal balloon dilator. one prosthesis consisted of radiopaque umbilical tape with a pleat secured by four 8-0 silk sutures thinly coated with silicone type a medical adhesive. this design was used to band the main pulmonary artery in five dogs, the subclavian artery in five, and the aorta in one. the second prosthesis, a stainless steel fatigued helix encased in a siliconized shield, was used to band the main pulmo ...19872954182
[genetic characteristics and protective properties of neamin-resistant mutant salmonella typhimurium and s. dublin of the nea(r) str(s) and nea(r) str(r) 500 classes].the genetic analysis of attenuated mutants, class nea(r) str(s), with the use of bacteriophage p 22 has shown that mutation rendering the mutants resistant to neamine is localized in gene nea a. in experiments with the intraperitoneal infection of mice, the appearance of this mutation in s. typhimurium and s. dublin virulent strains has been found to lead to the decrease of virulence in 100% of clones. on the basis of the data obtained in this investigation, region str-spc in s. typhimurium and ...19872955615
[role of the m. canis carrier state of healthy animals in the epidemiology of zoonotic microsporum infection]. 19872956788
possible cellular mechanism for cerebral vasospasm after experimental subarachnoid hemorrhage in the dog.this study was undertaken to examine some of the cellular ionic mechanisms responsible for the cerebral vasospasm that occurs as a consequence of subarachnoid hemorrhage (sah). after cisternal injection of autologous blood we documented spasm of the basilar artery upon angiography from 4 to 7 d postictus in six dogs. when these basilar arteries were isolated we observed marked membrane depolarization and enhanced electrical spike activity compared with controls. the slope of the membrane potenti ...19872957393
the use of angiodynography to quantify blood flow in the canine aorta.previous attempts to quantify blood flow by means of ultrasound have been hampered by inaccurate diameter measurements and failure to account for the parabolic nature of the cross-sectional velocity profile. angiodynography (adg) provides a b-mode image of vessel walls, and the outer flow envelope is constantly monitored to provide phasic diameter measurements throughout the cardiac cycle. frequency shifts are color-coded to provide a real-time visual display of varying velocity patterns across ...19872957515
experimental evaluation of a new albumin-impregnated knitted dacron prosthesis.the performance of a new albumin-impregnated, knitted vascular graft stored damp in saline was assessed by a comparative study in the canine aorta. knitted grafts interposed into the descending thoracic aorta for 4 weeks included: albumin-coated and stored damp in saline (damp albuminated) (n = 6), albumin-coated and freeze-dried (dry albuminated) (n = 5), collagen-coated (n = 4), and uncoated (n = 4). the results of macroscopic and microscopic evaluation were subjected to one-way analysis of va ...19872962524
partial transformation of mouse fibroblastic and epithelial cell lines with the v-myc oncogene.to investigate the role of the myc gene in mammalian cell transformation, plasmid constructs containing the v-myc oncogene and a co-selectable g418 resistance marker were introduced into both mouse fibroblasts (nih-3t3) and bladder epithelial cells (bbn3 and bbn7). after transfection or microinjection of dna, no transformed foci could be detected on confluent monolayers but, when the cells were cultured under conditions in which individual cells were allowed to grow and form colonies, morphologi ...19873023119
recombinant human tissue-type plasminogen activator therapy in acute thromboembolic stroke.systemic fibrinolytic therapy for acute stroke is no longer recommended because of resulting systemic fibrinolysis and the risk of intracerebral hemorrhage. human tissue-type plasminogen activator (tpa) is a native enzyme that converts plasminogen to plasmin with subsequent clot lysis. the affinity for plasminogen is increased several-fold when the substrate is bound to fibrin. at appropriate dosage, "clot-specific" thrombolysis may be achieved at the surface of the thrombus without creating sys ...19873112328
histological changes following transplantation of developing teeth to more advanced functional positions. 19873113409
efficacy of a polyvalent protein-polysaccharide dysentery oral vaccine. 19873115716
investigation of a health care worker with symptomatic human immunodeficiency virus infection: an epidemiologic approach. 19873116462
the inhibition by valproic acid of the mitochondrial oxidation of monocarboxylic and omega-hydroxymonocarboxylic acids: possible implications for the metabolism of gamma-aminobutyric acid.the interactions of 1-5 mm valproic acid with the hepatic fatty acid oxidation are here described. valproic acid was not substrate for hepatic peroxisomal fatty acid oxidation. its activation outside the mitochondrial matrix compartment was poor when compared to that of octanoic acid, a fatty acid containing the same number of carbones. valproic acid did not inhibit the fatty acyl-coa oxidase nor the cyanide-insensitive acyl-coa oxidation. valproic acid inhibited the mitochondrial oxidations of ...19873117781
a new immunomodulating compound (as-101) with potential therapeutic application.there has been interest in the potential of synthetic compounds to modify immune responses by imitation of cytokine action. direct administration of interleukin 2 (il-2) in conjunction with adoptive transfer of lymphokine activated killer cells has been used in the treatment of cancer, but there are toxic effects resulting from the high doses of il-2 required. we have developed a new synthetic compound, ammonium tri-chloro(dioxoethylene-o,o'-)tellurate (as-101), which has immunomodulating proper ...19873118216
gonadotropin regulation of tissue-type and urokinase-type plasminogen activators in rat granulosa and theca-interstitial cells during the periovulatory period.plasminogen activators (pas) are believed to be involved in ovulation. because both tissue-type plasminogen activator (tpa) and urokinase-type plasminogen activator (upa) are secreted by cultured rat granulosa cells, we have examined the activities of these proteins in ovarian homogenates as well as granulosa and theca-interstitial (ti) cells during gonadotropin-induced ovulation. immature rats were injected with 20 iu pregnant mare serum gonadotropin (pmsg) to initiate follicle development, fol ...19873121412
evaluation of the 11co2 positron emission tomographic method for measuring brain ph. i. ph changes measured in states of altered pco2.the 11co2 method for measuring local brain ph with positron emission tomography (pet) has been experimentally evaluated, testing the adequacy of the kinetic model and the ability of the method to measure changes in brain ph. plasma and tissue time/activity curves measured during and following continuous inhalation of 11co2 were fit with a kinetic model that includes effects of tissue ph, blood flow, and fixation of co2 into compounds other than dissolved gas and bicarbonate ions. for each of ten ...19873121647
lactate dehydrogenase and malate dehydrogenase of sertoli cells in rats.lactate-dehydrogenase (ldh) and malate-dehydrogenase (mdh) activities and isozyme patterns of maturing sertoli cells were investigated in cultured cells obtained from 2-5-week-old rats. both enzymes, and more strikingly ldh, increased between 2 and 3 weeks of age. ldh isozyme complement included all five isozymes and, in the more mature rats, had a shift toward the isoforms rich in subunit. these data are consistent with an increasing production of lactate/pyruvate and with a finer regulation of ...19873122678
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