Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| phenotypic characterization of human pathogenic bacteria in fish from the coastal waters of south west cameroon: public health implications. | increasing economic and recreational opportunities, attractive scenery and a perception of a better quality of life are luring people to the coast. unfortunately, these activities together with the commensurate increase in population in the area inevitably result in pollution of coastal waters with excessive microorganisms and other pollutants. microbial pollutants not only contaminate the coastal water but also aquatic food sources, thus posing a health risk to consumers. fish is a major source ... | 2009 | 19658320 |
| involvement of t helper type 17 and regulatory t cell activity in citrobacter rodentium invasion and inflammatory damage. | citrobacter rodentium is a murine pathogen that transiently colonizes the lumen of the large intestine. c. rodentium induces colitis, but the relative importance and temporal induction of the t helper type 17 (th17) and regulatory t cell (t(reg)) pathways in protection from the infection and inflammation have not been assessed. our aim was to investigate the key immunological signalling events associated with successful clearance of c. rodentium. mice were challenged with luminescent-tagged c. r ... | 2009 | 19659780 |
| vasoactive intestinal peptide ameliorates intestinal barrier disruption associated with citrobacter rodentium-induced colitis. | attaching and effacing bacterial pathogens attach to the apical surface of epithelial cells and disrupt epithelial barrier function, increasing permeability and allowing luminal contents access to the underlying milieu. previous in vitro studies demonstrated that the neuropeptide vasoactive intestinal peptide (vip) regulates epithelial paracellular permeability, and the high concentrations and close proximity of vip-containing nerve fibers to intestinal epithelial cells would support such a func ... | 2009 | 19661153 |
| screening, purification, and identification of the enzyme producing n-(l-alpha-l-aspartyl)-l-phenylalanine methyl ester from l-isoasparagine and l-phenylalanine methyl ester. | screening was carried out for microorganisms able to produce n-(l-alpha-l-aspartyl)-l-phenylalanine methyl ester [apm] from l-isoasparagine and l-phenylalanine methyl ester hydrochloride. of the 422 strains examined, 44 strains belonging to the family enterobacteriaceae were found to produce apm. the enzyme catalyzing apm production was purified and identified as dipeptidase e. | 2009 | 19664550 |
| th17 cytokines and host-pathogen interactions at the mucosa: dichotomies of help and harm. | the mucosal surfaces are often the first site of interaction between pathogenic microorganisms and the host. activation of the mucosal immune response has the important function of containing an infection and preventing dissemination of pathogens to systemic sites (barrier function). numerous lines of evidence suggest that the barrier function is orchestrated by a subset of cytokines (interleukin (il-)17 and il-22), which belong to the th17 family. il-17 and il-22 induce expression of antimicrob ... | 2009 | 19665391 |
| [antimicrobial activity of tebipenem against various clinical isolates from various specimen, mainly urinary tract]. | tebipenem is the active metabolite of me1211, tebipenem pivoxil, a novel oral carbapenem that possesses potent activity against almost pathogens except for pseudomonas aeruginosa. in this study, we compared the susceptibility of tebipenem with current antibiotics against various organisms isolated from various specimen, mainly urinary tract. tebipenem had a potent activity against neisseria gonorrhoeae; its activity was comparable to it of cefixime that has most potent activity among oral antibi ... | 2009 | 19673353 |
| cxcr2-dependent mucosal neutrophil influx protects against colitis-associated diarrhea caused by an attaching/effacing lesion-forming bacterial pathogen. | enteropathogenic escherichia coli (epec) is a major cause of diarrheal disease in young children, yet symptoms and duration are highly variable for unknown reasons. citrobacter rodentium, a murine model pathogen that shares important functional features with epec, colonizes mice in colon and cecum and causes inflammation, but typically little or no diarrhea. we conducted genome-wide microarray studies to define mechanisms of host defense and disease in c. rodentium infection. a significant fract ... | 2009 | 19675161 |
| hidden qnrb12 gene in a finnish faecal microbiota isolate from 1994. | 2009 | 19692413 | |
| bacteria isolated from 25 hydatid cysts in sheep, cattle and goats. | bacteria were isolated from 12 of 25 hydatid cysts collected from the lungs and livers of cattle, sheep and goats slaughtered in the province of messina, sicily, italy. citrobacter freundii was isolated from seven of the cysts, aeromonas hydrophila from three, staphylococcus species from two, salmonella species from two and escherichia coli and proteus vulgaris from one. | 2009 | 19700784 |
| comparative study of anthocyanin composition, antimicrobial and antioxidant activity in bilberry (vaccinium myrtillus l.) and blueberry (vaccinium corymbosum l.) fruits. | simultaneous comparison of bilberry (vaccinium myrtillus l.) and blueberry (vaccinium corymbosum l) fruits for their anthocyanin composition, antimicrobial and antioxidant activity is reported. the aim of this study was to investigate and to compare anthocyanin composition, antimicrobial and antioxidant activity in bilberry and blueberry fruits and their skins. the investigations revealed that the highest amount of total anthocyanins was observed in fruits skins of blueberry cultivars. the resul ... | 2009 | 19702172 |
| an outer membrane protease of the omptin family prevents activation of the citrobacter rodentium phopq two-component system by antimicrobial peptides. | the phopq two-component system of the intracellular pathogen salmonella enterica senses and controls resistance to alpha-helical antimicrobial peptides (amps) by regulating covalent modifications of lipid a. a homologue of the phopq operon was found in the genome of the murine enteric extracellular pathogen, citrobacter rodentium. here we report that c. rodentium phopq was apparently unable to mediate activation of target genes in the presence of alpha-helical amps. however, these amps activated ... | 2009 | 19708916 |
| [formation of biofilms by fecal strains of enterobacteria and yeast fungi from candida genus]. | to assess the ability of bacteria and candida genus' yeast fungi from human fecal microflora to form biofilms. | 2009 | 19715202 |
| sulfate reduction and copper precipitation by a citrobacter sp. isolated from a mining area. | a strain of sulfate-reducing bacteria, designated strain 'dbm', was isolated from sediments of a mining area. phylogenetic analysis of the 16s rrna gene sequence of the isolate revealed that it was related to members of the genus citrobacter, with c. azor-4, c. freundii, c. braakii and c. werkmanii being the most closely related species (sequence similarity up to 98%). few studies have been done on sulfate reduction ability in citrobacter. electron microscopy studies showed that the morphology o ... | 2009 | 18977087 |
| role of rpos in the virulence of citrobacter rodentium. | citrobacter rodentium is a mouse enteropathogen that is closely related to escherichia coli and causes severe colonic hyperplasia and bloody diarrhea. c. rodentium infection requires expression of genes of the locus of enterocyte effacement (lee) pathogenicity island, which simulates infection by enteropathogenic e. coli and enterohemorrhagic e. coli in the human intestine, providing an effective model for studying enteropathogenesis. in this study we investigated the role of rpos, the stationar ... | 2009 | 18981255 |
| optimization of process parameters for the production of tyrosine phenol lyase by citrobacterfreundii mtcc 2424. | the process optimization using technological combinations for the production of tyrosine phenol lyase by citrobacter freundii mtcc 2424 has been carried out in this study. the maximum production of tyrosine phenol lyase (0.15 u) was obtained by culturing c. freundii mtcc 2424 in a medium containing (g/l) meat extract 5.0, yeast extract 5.0, peptone 2.5, and l-tyrosine 1.0 at 25 degrees c for 16 h in a temperature controlled orbital shaker. a 2.5-fold increase in enzyme activity with 1.3-fold dec ... | 2009 | 18993058 |
| beta-catenin stabilization imparts crypt progenitor phenotype to hyperproliferating colonic epithelia. | utilizing the citrobacter rodentium (cr)-induced transmissible murine colonic hyperplasia (tmch) model, we provide mechanistic basis of changes in beta-catenin/apc/ckiepsilon leading to progression and/or regression of hyperplasia in vivo. in response to cr-induced tmch, crypt lengths increased significantly between days 6-27 post-infection, followed by a steep decline by day 34. beta-cat(45)/total beta-catenin were elevated on day 1 post-infection, preceding changes in crypt length, and persist ... | 2009 | 18996369 |
| espt triggers formation of lamellipodia and membrane ruffles through activation of rac-1 and cdc42. | subversion of the eukaryotic cell cytoskeleton is a virulence strategy employed by many bacterial pathogens. due to the pivotal role of rho gtpases in actin dynamics they are common targets of bacterial effector proteins and toxins. ipgb1, ipgb2 (shigella), sifa, sifb (salmonella) and map and espm (attaching and effacing pathogens) constitute a family of type iii secretion system effectors that subverts small gtpase signalling pathways. in this study we identified and characterized espt from cit ... | 2009 | 19016787 |
| regulation of hepatic cytochrome p450 expression in mice with intestinal or systemic infections of citrobacter rodentium. | we reported previously that infection of c3h/heouj (heou) mice with the murine intestinal pathogen citrobacter rodentium caused a selective modulation of hepatic cytochrome p450 (p450) gene expression in the liver that was independent of the toll-like receptor 4. however, heou mice are much more sensitive to the pathogenic effects of c. rodentium infection, and the p450 down-regulation was associated with significant morbidity in the animals. here, we report that oral infection of c57bl/6 mice w ... | 2009 | 18971315 |
| a survey on species and prevalence rate of bacterial agents isolated from cockroaches in three hospitals. | the presence of cockroaches has health implications, such as nosocomial infection, as the insects move freely from areas within and around hospitals that may harbor pathogenic organisms. the goals of the present study were to determine species of bacteria isolated from cockroaches found in three tehran hospitals, and to determine distribution of potential vectors by species and sex. the study is descriptive laboratory research. a total of 305 cockroaches from three species were trapped and ident ... | 2009 | 18973441 |
| [emergence of vancomycin-dependent enterococci following glycopeptide therapy: case report and review]. | outbreaks of vancomycin-resistant enterococci have been increasingly reported in france over the last three years. we report here, the emergence of a vancomycin-dependent enterococci isolate following glycopeptide therapy. | 2009 | 18845404 |
| physicochemical treatments of anionic surfactants wastewater: effect on aerobic biodegradability. | the effect of different physicochemical treatments on the aerobic biodegradability of an industrial wastewater resulting from a cosmetic industry has been investigated. this industrial wastewater contains 11423 and 3148mgl(-1) of chemical oxygen demand (cod) and anionic surfactants, respectively. the concentration of cod and anionic surfactants were followed throughout the diverse physicochemical treatments and biodegradation experiments. different pretreatments of this industrial wastewater usi ... | 2009 | 18799262 |
| synthesis, characterization and antibacterial activity of some triazole mannich bases carrying diphenylsulfone moieties. | a series of mannich bases of 4-substituted 5-[4-(4-x-phenylsulfonyl)phenyl]-2,4-dihydro-3h-1,2,4-triazole-3-thiones, x=h, cl, br, 3 and 5 were synthesized and characterized on the basis of ir, nmr and elemental analyses. the potential antibacterial effects of the synthesized compounds were investigated using the acinetobacter baumanii atcc 19606; citrobacter freundii atcc 8090; pseudomonas aeruginosa atcc 9027; enterococcus faecalis atcc 19433; staphylococcus aureus atcc 12600; staphylococcus ep ... | 2009 | 18708273 |
| ecthyma gangrenosum with citrobacter freundii infection. | 2009 | 18759794 | |
| citrobacter infections in a general hospital: characteristics and outcomes. | in this investigation, we sought to investigate the characteristics of citrobacter spp. infections. a retrospective cohort study in a 700-bed, tertiary care, university hospital was carried out during the period from june 1994 to january 2006. seventy-eight patients (70 adults) with citrobacter spp. isolates were identified. c. freundii was more common (71.8%), followed by c. koseri (23.1%) and c. braakii (3.8%). the most common associated clinical syndromes were urinary tract infections (52.6% ... | 2009 | 18682995 |
| study of the bacterial ecosystem in tropical cooked and peeled shrimps using a polyphasic approach. | the characterization of the microbial ecosystem of cooked tropical shrimps was carried out using a polyphasic approach. first, culture-dependent methods were used for bacterial enumeration and the phenotypic and molecular identification of bacterial isolates. then, culture-independent methods, including pcr-ttge (v3 region of the 16s rrna gene), provided a fingerprinting of bacterial dna directly extracted from shrimps. two batches of cooked and peeled tropical shrimps were stored at 5 and 15 de ... | 2009 | 18573557 |
| citrobacter koseri meningitis: a neurosurgical condition? | a 2-month-old girl developed meningitis, ventriculitis and brain abscess in the course of citrobacter koseri infection. she was successfully treated with the combined use of antibiotics, intra-cavitary urokinase and surgery, thus avoiding the development of hydrocephalus and of ventricular loculation. c. koseri is a gram-negative pathogen with a strong predilection for the neonatal brain. brain abscesses develop in roughly 77% of cases, causing severe neurological sequels in one-half and death i ... | 2010 | 19717321 |
| prevalence of pseudomonas aeruginosa in post-operative wound infection in a referral hospital in haryana, india. | the objective of our study was to determine the prevalence of pseudomonas aeruginosa in the isolates of postoperative wound and its susceptibility pattern to commonly used antibiotics. | 2010 | 21346900 |
| akara ogbomoso: microbiological examination and identification of hazards and critical control points. | akara ogbomoso was examined toward the establishment of hazard analysis and critical control point (haccp). the akara was produced in residential buildings with the attendant consequence of contamination. there was ample growth of aerobes, coliforms, staphylococci, shigella and yeast/mold from the samples, water and cowpea pastes. microbial contaminations occur through the processing, which can be corrected through education by adopting good hygienic and manufacturing practices. the critical con ... | 2010 | 21339157 |
| [genetic environments of bla(ctx-m) genes located on conjugative plasmids of enterobacteriaceae nosocomial isolates collected in russia within 2003-2007]. | the study showed that bla(ctx-m) genes were present in the genomes of 71% of cephalosporin resistant enterobacteriaceae nosocomial isolates (n=833) collected in russian hospitals within 2003-2007, including 91% of e.coli, 90% of klebsiella spp., 38% of enterobacter spp., 31% of citrobacter spp. (n=9), and 36% of the other enterobacteriaceae species. the genes belonging to the following subtypes (clusters) were identified: bla(ctx-m-1) (529 bla(ctx-m-15) genes; 25 bla(ctx-m-3) genes; 1 bla(ctx-m- ... | 2010 | 21574418 |
| comparative study of biological hydrogen production by pure strains and consortia of facultative and strict anaerobic bacteria. | in this paper, a simple and rapid method was developed in order to assess in comparative tests the production of binary biogas mixtures containing co(2) and another gaseous compound such as hydrogen or methane. this method was validated and experimented for the characterisation of the biochemical hydrogen potential of different pure strains and mixed cultures of hydrogen-producing bacteria (hpb) growing on glucose. the experimental results compared the hydrogen production yield of 19 different p ... | 2010 | 21185171 |
| enteropathogenic and enterohemorrhagic escherichia coli type iii secretion effector espv induces radical morphological changes in eukaryotic cells. | enteropathogenic escherichia coli (epec) and enterohemorrhagic escherichia coli (ehec) are important human pathogens that rely on translocation of type iii secretion system (t3ss) effectors for subversion of signal transduction pathways and colonization of the mammalian gut mucosa. while a core set of effectors is conserved between epec and ehec strains, a growing number of accessory effectors that were found at various frequencies in clinical and environmental isolates have been recently identi ... | 2010 | 21189318 |
| strain-resolved community genomic analysis of gut microbial colonization in a premature infant. | the intestinal microbiome is a critical determinant of human health. alterations in its composition have been correlated with chronic disorders, such as obesity and inflammatory bowel disease in adults, and may be associated with neonatal necrotizing enterocolitis in premature infants. increasing evidence suggests that strain-level genomic variation may underpin distinct ecological trajectories within mixed populations, yet there have been few strain-resolved analyses of genotype-phenotype conne ... | 2010 | 21191099 |
| cd4(+) lymphoid tissue-inducer cells promote innate immunity in the gut. | fetal cd4(+) lymphoid tissue inducer (lti) cells play a critical role in the development of lymphoid tissues. recent studies identified that lti cells persist in adults and are related to a heterogeneous population of innate lymphoid cells that have been implicated in inflammatory responses. however, whether lti cells contribute to protective immunity remains poorly defined. we demonstrate that after infection with citrobacter rodentium, cd4(+) lti cells were a dominant source of interleukin-22 ... | 2010 | 21194981 |
| virulence regulation in citrobacter rodentium: the art of timing. | the mouse enteric pathogen citrobacter rodentium, like its human counterpart, enteropathogenic escherichia coli, causes attaching and effacing lesions in the intestinal epithelium of its host. this phenotype requires virulence factors encoded by the locus for enterocyte effacement (lee) pathogenicity island. for timely expression of these virulence determinants at the site of infection and for efficient delivery of some virulence factors into epithelial cells, c. rodentium utilizes a positive re ... | 2010 | 21255326 |
| incidence of fever and bacteriemia following flexible fiberoptic bronchoscopy: a prospective study. | the latest american heart association (aha) statement for preventing infectious endocarditis, has not recommended prophylactic antibiotic therapy prior to fiberoptic bronchoscopy (fb) except for patients with preexisting predisposing cardiac conditions. our aim was to determine the incidence of bacteriemia and fever following fb in our experience and compare with those which have been mentioned in aha guideline as well as other studies. venous blood of 85 consecutive patients was evaluated for b ... | 2010 | 21287478 |
| antimicrobial susceptibility and multiplex pcr screening of ampc genes from isolates of enterobacter cloacae, citrobacter freundii, and serratia marcescens. | the emergence of multiple drug resistance in enterobacteriaceae is of particular concern. the aim of this study was to evaluate the antimicrobial susceptibility and screen for the ampc gene in three members of the enterobacteriaceae family (enterobacter cloacae, citrobacter freundii, and serratia marcescens) found at taichung veterans general hospital during the past 5 years using multiplex polymerase chain reaction (pcr). | 2010 | 21291844 |
| microbial evaluation and public health implications of urine as alternative therapy in clinical pediatric cases: health implication of urine therapy. | cultural means of pediatric treatment during ill health is a mainstay in africa, and though urine has been known to contain enteric pathogens, urine therapy is still culturally applicable in some health conditions and also advocated as alternative therapy. the study therefore, is to evaluate the microbial contents and safety of urine. | 2010 | 21293739 |
| normal organ weights, serum chemistry, hematology, and cecal and nasopharyngeal bacterial cultures in the gray short-tailed opossum (monodelphis domestica). | gray short-tailed opossums (monodelphis domestica) currently are used in genetic, developmental, oncology, and neurologic research. little is known about their natural flora or potential for pathogenic infectious disease. the present study aims to improve existing comparative normal blood and organ weight values available to researchers and to describe flora of clinically normal m. domestica to obtain an understanding of potential pathogenic flora in clinically abnormal animals. for evaluation o ... | 2010 | 20819383 |
| [implication of extended-spectrum beta-lactamase enterobacteriaceae in nosocomial infections in neonates]. | the objective of this study was to determine the incidence of extended-spectrum beta-lactamase (esbls) enterobacteriaceae colonization and infection in hospitalized children. | 2010 | 20826324 |
| phytoremediation of mercury in pristine and crude oil contaminated soils: contributions of rhizobacteria and their host plants to mercury removal. | the rhizospheric soils of three tested legume crops: broad beans (vicia faba), beans (phaseolus vulgaris) and pea (pisum sativum), and two nonlegume crops: cucumber (cucumis sativus) and tomato, (lycopersicon esculentum) contained considerable numbers (the magnitude of 10(5)g(-1) soil) of bacteria with the combined potential for hydrocarbon-utilization and mercury-resistance. sequencing of the 16s rrna coding genes of rhizobacteria associated with broad beans revealed that they were affiliated t ... | 2010 | 20833430 |
| antibiotic susceptibility pattern in urinary isolates of gram negative bacilli with special reference to ampc β-lactamase in a tertiary care hospital. | resistance to higher antimicrobial agent is commonly seen in gram negative bacilli. this issue is a challenging problem to the medical practitioners in addition to it is financial impact on the health care system. | 2010 | 20842250 |
| detection of kpc-2-producing citrobacter freundii isolates in spain. | 2010 | 20851814 | |
| prevalence of bacteria and parasites in white ibis in egypt. | a field survey was conducted to evaluate the prevalence of bacterial infections among free-living white ibis (nipponia nippon) in which 92 bacterial isolates were recovered from 193 different internal organs of 55 apparently healthy ibis. escherichia coli and salmonella spp. were isolated at rates of 43.6% and 14.5%, respectively. the other bacterial pathogens isolated were shigella spp. (34.5%), enterobacter spp. (21.8%) citrobacter spp. (18.1%), klebsiella pneumonia (16.3), staphylococcus aure ... | 2010 | 20857378 |
| isolation and characterization of bacteria from the gut of bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion. | bombyx mori l. (lepidoptera: bombycidae) have been domesticated and widely used for silk production. it feeds on mulberry leaves. mulberry leaves are mainly composed of pectin, xylan, cellulose and starch. some of the digestive enzymes that degrade these carbohydrates might be produced by gut bacteria. eleven isolates were obtained from the digestive tract of b. mori, including the gram positive bacillus circulans and gram negative proteus vulgaris, klebsiella pneumoniae, escherichia coli, citro ... | 2010 | 20874394 |
| resistance phenotype-genotype correlation and molecular epidemiology of citrobacter, enterobacter, proteus, providencia, salmonella and serratia that carry extended-spectrum β-lactamases with or without plasmid-mediated ampc β-lactamase genes in thailand. | extended-spectrum β-lactamases (esbls) and plasmid-mediated ampc β-lactamases (pampcs) have been increasingly reported among less commonly encountered genera of enterobacteriaceae. however, little is known regarding the genetic characteristics of resistance genes and epidemiology of these genera. lack of accurate esbl and pampc detection may adversely affect therapeutic outcomes. this study investigated resistance phenotype-genotype correlation and molecular epidemiology among six genera of ente ... | 2010 | 20880563 |
| alimentary tract bacteria isolated and identified with api-20e and molecular cloning techniques from australian tropical fruit flies, bactrocera cacuminata and b. tryoni. | bacteria were isolated from the crop and midgut of field collected bactrocera cacuminata (hering) and bactrocera tryoni (froggatt) (diptera: tephritidae). two methods were used, firstly isolation onto two types of bacteriological culture media (pyea and tsa) and identification using the api-20e diagnostic kit, and secondly, analysis of samples using the 16s rrna gene molecular diagnostic method. using the api-20e method, 10 genera and 17 species of bacteria in the family enterobacteriaceae were ... | 2010 | 20883132 |
| characterization of the enterobacteriaceae community that developed during storage of minced beef under aerobic or modified atmosphere packaging conditions. | the whole cell protein and macrorestriction analysis of dna of enterobacteriaceae isolates recovered from minced beef stored at 0, 5, 10 and 15 °c aerobically and under modified atmosphere packaging consisting of 40% co(2)-30% o(2)-30% n(2) in the presence (map+) and absence (map-) of oregano essential oil were studied. sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) profiles obtained from whole cell protein analysis of the enterobacteriaceae isolates revealed seven groups. ... | 2010 | 21216482 |
| arsenite-oxidizing and arsenate-reducing bacteria associated with arsenic-rich groundwater in taiwan. | drinking highly arsenic-contaminated groundwater is a likely cause of blackfoot disease in taiwan, but microorganisms that potentially control arsenic mobility in the subsurface remain unstudied. the objective of this study was to investigate the relevant arsenite-oxidizing and arsenate-reducing microbial community that exists in highly arsenic-contaminated groundwater in taiwan. we cultured and identified arsenic-transforming bacteria, analyzed arsenic resistance and transformation, and determi ... | 2010 | 21216490 |
| antibiotic resistant bacteria as bio-indicator of polluted effluent in the green turtles, chelonia mydas in oman. | antibiotic resistant bacteria were studied as bio-indicators of marine polluted effluents during egg-laying in green turtles. a non-invasive procedure for sampling oviductal fluid was used to test for exposure of turtles to pollution in ras al-hadd, oman, which is one of the most important nesting beaches in the world. each sample was obtained by inserting a 15 cm sterile swab gently into the cloacal vent as the sphincter muscle is relaxed and the cloacal lining is unfolded to the outside. forty ... | 2010 | 21237506 |
| mind-bending infections. | in 1770, wolfgang von kempelen presented his famous chess-playing automaton, "the turk," at the court of maria theresa, the austro-hungarian empress. the instrument consisted of a life-size, pipe-wielding, turbaned model of a stereotypical turk seated behind a cabinet table, his right hand resting next to a chess board. "the turk" played a reasonably good game of chess, its mechanical arms deftly grasping the chessmen and moving them about the board. opening the doors of the cabinet, one could s ... | 2010 | 21637012 |
| determination of spatial and temporal colonization of enteropathogenic e. coli and enterohemorrhagic e. coli in mice using bioluminescent in vivo imaging. | infectious diarrhea is a major contributor of child morbidity and mortality in developing nations. murine models to study the pathogenesis of infectious diarrhea caused by organisms such as enteropathogenic e. coli (epec) and enterohemorrhagic e. coli (ehec) are not fully characterized. more emphasis has been placed on infection of mice with the murine specific pathogen citrobacter rodentium. while these three organisms are genetically related they are not identical. our goal was to better chara ... | 2010 | 21637016 |
| crystal structure of the ampr effector binding domain provides insight into the molecular regulation of inducible ampc beta-lactamase. | hyperproduction of ampc beta-lactamase (ampc) is a formidable mechanism of resistance to penicillins and cephalosporins in gram-negative bacteria such as pseudomonas aeruginosa and enterobacteriaceae. ampc expression is regulated by the lysr-type transcriptional regulator ampr. ampr and ampc genes form a divergent operon with overlapping promoters to which ampr binds and regulates the transcription of both genes. ampr induces ampc by binding to one member of the family of 1,6-anhydro-n-acetylmur ... | 2010 | 20594961 |
| guideline for phenotypic screening and confirmation of carbapenemases in enterobacteriaceae. | adequate detection of carbapenemase-producing enterobacteriaceae is crucial for infection control measures and appropriate choice of antimicrobial therapy. this guideline aims to improve the detection of carbapenemase-producing enterobacteriaceae in the routine setting of clinical microbiology laboratories. detection of carbapenemases in enterobacteriaceae includes a screening step followed by a genotypic and optional phenotypic confirmatory step. for all enterobacteriaceae, the meropenem screen ... | 2010 | 20598859 |
| interleukin-11 reduces tlr4-induced colitis in tlr2-deficient mice and restores intestinal stat3 signaling. | the roles of intestinal toll-like receptors (tlr) in the pathogenesis of colitis are not known. tlr2 and tlr4 appear to protect against dextran sodium sulfate-induced colitis by promoting mucosal integrity, but it is not clear whether this method of protection occurs in other models of colitis. we investigated the roles of tlr2 and tlr4 and the cell types that express these receptors during infectious colitis. | 2010 | 20600022 |
| modulation of hepatic cytochrome p450s by citrobacter rodentium infection in interleukin-6- and interferon-{gamma}-null mice. | after infection with citrobacter rodentium, murine hepatic cytochrome p450 (p450) mrnas are selectively regulated. several serum proinflammatory cytokines are elevated, the most abundant being interleukin-6 (il6). to elucidate the role of cytokines in the regulation of p450s during infection, we orally infected wild-type, il6(-/-), or interferon-γ(-/-) [ifnγ(-/-)] female c57bl/6j mice with c. rodentium and analyzed hepatic p450 expression 7 days later. the majority of p450 mrnas were equally aff ... | 2010 | 20719939 |
| chemical composition and antibacterial activity of essential oils of tripleurospermum disciforme in three developmental stages. | tripleurospermum disciforme (c.a. mey) schultz bip. (asteraceae) is a widespread biennial species which also has traditional medicinal uses. according to the few recent studies, essential oils of this species exhibit anti-inflammatory, antispasmodic, antiseptic, antifungal, antiulcer, and antioxidant activity. objective: the chemical compositions of the hydrodistilled oils of t. disciforme of iranian origin are studied in the stages of prior to flowering, flowering, and post flowering, for the f ... | 2010 | 20795784 |
| nosocomial diarrhoea in relation to sanitation state: a study in tikrit, iraq. | a cross-sectional hospital-based study of 259 children aged < 5 years was carried out in tikrit, iraq, to identify the prevalence of nosocomial diarrhoea and sources of contamination in the ward environment. nosocomial diarrhoea was diagnosed in 84 children (32.4%). children with diarrhoea were more likely than unaffected children to be bottle-fed, given unboiled water, to have unclean food containers and contaminated bed sheets. three out of 5 brands of formula milk (unopened cans) were contami ... | 2010 | 20799556 |
| sensitization of microcin j25-resistant strains by a membrane-permeabilizing peptide. | microcin j25 (mccj25) is a plasmid-encoded, 21-amino-acid, antibacterial peptide produced by escherichia coli. mccj25 inhibits rna polymerase and the membrane respiratory chain. mccj25 uptake into e. coli-sensitive strains is mediated by the outer membrane receptor fhua and the inner membrane proteins tonb, exbb, exbd, and sbma. this peptide is active on some e. coli, salmonella, and shigella species strains, while other gram-negative bacteria, such as clinical isolates of enterobacter cloacae, ... | 2010 | 20802073 |
| espj effector in enterohemorrhagic e. coli translocates into host mitochondria via an atypical mitochondrial targeting signal. | ehec is a bacterial pathogen causing diarrhea and hemorrhagic colitis in humans. to exert virulence, ehec exploits a subset of effectors that are translocated into host cells via the type iii secretion system. espj, which was recently identified as a type iii secreted effector, is conserved in related pathogens such as epec and citrobacter rodentium. however, the exact function of espj remains unclear. in the present study, we found that espj was unstable in host cells, which might be attributab ... | 2010 | 20618683 |
| production of antimicrobial silver nanoparticles in water extracts of the fungus amylomyces rouxii strain ksu-09. | a fungal strain, ksu-09, isolated from the roots of date palm (phoenix dactylifera), was identified as amylomyces rouxii based on sequence analysis of the internal transcribed spacer (its) region of its rrna genes. mycelia-free water extracts obtained from mycelium suspended in water for 72h facilitated the production of stable, predominantly monodispersed and spherical silver nanoparticles (agnps) in the size range of 5-27nm upon addition of 1mm silver nitrate, as determined by the xrd, afm and ... | 2010 | 20619641 |
| survival of campylobacter jejuni under conditions of atmospheric oxygen tension with the support of pseudomonas spp. | campylobacter jejuni is a major food-borne pathogen. despite causing enteritis in humans, it is a well-adapted intestinal microorganism in animals, hardly ever generating disease symptoms. nevertheless, as a true microaerophilic microorganism it is still puzzling how campylobacter cells can survive on chicken meat, the main source of human infection. in this study, we demonstrate that c. jejuni is able to withstand conditions of atmospheric oxygen tension when cocultured with pseudomonas species ... | 2010 | 20639377 |
| osteopontin mediates citrobacter rodentium-induced colonic epithelial cell hyperplasia and attaching-effacing lesions. | although osteopontin (opn) is up-regulated in inflammatory bowel diseases, its role in disease pathogenesis remains controversial. the objective of this study was to determine the role of opn in host responses to a non-invasive bacterial pathogen, citrobacter rodentium, which serves as a murine infectious model of colitis. opn gene knockout and wild-type mice were infected orogastrically with either c. rodentium or luria-bertani (lb) broth. mouse-derived opn(+/+) and opn(-/-) fibroblasts were in ... | 2010 | 20651246 |
| pyomyoma in a premenopausal woman with fever of unknown origin. | pyomyoma (suppurative leiomyoma) is a rare disease that is a serious complication. most cases have occurred in pregnant or postmenopausal women. | 2010 | 20664442 |
| production of biogenic amines "in vitro" in relation to the growth phase by enterobacteriaceae species isolated from traditional sausages. | histidine, lysine, ornithine and tyrosine decarboxylase activities were tested in 79 strains of enterobacteriaceae (41 of hafnia alvei, 17 of serratia liquefaciens, 5 of enterobacter cloacae, 4 of citrobacter braakii, 2 of proteus vulgaris, 2 of proteus mirabilis, 2 of providencia stuartii, 2 of klebsiella terrigena, 1 of rahnella aquatilis, 1 of salmonella arizonae, 1 of citrobacter youngae and 1 of escherichia coli) isolated from botillo, a spanish traditional sausage. in general, the strains ... | 2010 | 20673614 |
| evidence for a bimodal distribution of escherichia coli doubling times below a threshold initial cell concentration. | in the process of developing a microplate-based growth assay, we discovered that our test organism, a native e. coli isolate, displayed very uniform doubling times (tau) only up to a certain threshold cell density. below this cell concentration (<or= 100 -1,000 cfu ml-1 ; <or= 27-270 cfu well-1) we observed an obvious increase in the tau scatter. | 2010 | 20678197 |
| qnrb9 in association with tem-116 extended-spectrum beta-lactamase in citrobacter freundii isolated from sewage effluent: first report from italy. | the aim of the present study was to investigate the mechanism of quinolone and beta-lactam resistance in an isolate of citrobacter freundii pc2/08 collected from sewage effluent from l'aquila, italy. qnrb-9 and bla(tem-116 )were co-expressed in a large plasmid identified in c. freundi pc2/08 strain. compared to tem-1, tem-116 showed two single mutations: v84i and a184v. the plpc2/08 plasmid conferred resistance to several beta-lactams and fluoroquinolones. tazobactam could be considered a good i ... | 2010 | 20685627 |
| performance of a sulfidogenic bioreactor and bacterial community shifts under different alkalinity levels. | the performance of a sulfidogenic bioreactor and the response of bacterial populations to influent alkalinity changes were investigated. the bioreactor reached 40% of sulfate removal efficiency (sre) with 0 mg l(-1) of alkalinity, and single-stranded conformation polymorphism profiles showed that some members of bacteroides, dysgonomonas, sporobacter, quinella, and citrobacter became dominant populations. 16s rrna gene library analysis indicated that the actinobacteria group increased from 0% in ... | 2010 | 20688512 |
| bacteraemia caused by non-freundii, non-koseri citrobacter species in taiwan. | this study analysed the clinical characteristics of bacteraemia due to unusual citrobacter species. all non-freundii and non-koseri citrobacter isolates were identified to species level by two commercial identification methods and 16s rrna gene sequence analysis. a total of 306 patients with citrobacter spp. bacteraemia were identified from january 2000 through december 2009. four patients (1.3%) had c. braakii bacteraemia, and one had c. amalonaticus and c. sedlakii sepsis, respectively. miside ... | 2010 | 20708303 |
| novel changes in nf-{kappa}b activity during progression and regression phases of hyperplasia: role of mek, erk, and p38. | utilizing the citrobacter rodentium-induced transmissible murine colonic hyperplasia (tmch) model, we measured hyperplasia and nf-κb activation during progression (days 6 and 12 post-infection) and regression (days 20-34 post-infection) phases of tmch. nf-κb activity increased at progression in conjunction with bacterial attachment and translocation to the colonic crypts and decreased 40% by day 20. nf-κb activity at days 27 and 34, however, remained 2-3-fold higher than uninfected control. expr ... | 2010 | 20710027 |
| citrobacter koseri meningitis: another freediving risk? | we present a rare case of meningitis caused by citrobacter koseri in an immunocompetent adult who had recently been freediving. middle ear pressure changes from this recreational activity, and the subsequent inflammatory response, are likely to have provided this environmental organism access to the central nervous system, and thus the ability to cause clinically significant infection. | 2010 | 20933000 |
| structure of the o-polysaccharide of citrobacter youngae pcm 1503. | the o-polysaccharide (o-antigen) was obtained from the lipopolysaccharide of citrobacter youngae pcm 1503, which is currently classified to the o7 serogroup. the following structure of the tetrasaccharide repeating unit of the polysaccharide was established by sugar and methylation analyses along with 1d and 2d (1)h and (13)c nmr spectroscopy: structural and serological data of the o-antigen suggest that strain pcm 1503 should be reclassified to a new citrobacter serogroup. | 2010 | 20934177 |
| citrobacter freundii-induced cold agglutinin hemolysis. | 2010 | 20938661 | |
| inhibitory effects of lactic acid and lauricidin on spoilage organisms of chicken breast during storage at chilled temperature. | different concentrations of lauricidin (lu, containing 1% lactic acid) and lactic acid alone (la) were evaluated for their effectiveness in reducing naturally occurring microflora of raw chicken breasts. chicken breasts were dipped in 0 (control), 0.5, 1.0, 1.5, and 2.0% solutions of lu (w/v) or la (v/v) for 10, 20, and 30 min and stored at 4°c for 14 d. total plate counts (tpc) and populations of pseudomonas spp. and enterobacteriaceae were determined before and after dipping and after storing ... | 2010 | 20947197 |
| responding to infection and apoptosis--a task for th17 cells. | two of the critical cytokines required for the differentiation of t helper 17 (t(h)17) cells from naive cd4 t cells are transforming growth factor-beta (tgf-β) and interleukin-6 (il-6). innate recognition of apoptotic cells in the presence of toll-like receptor engagement directs the simultaneous synthesis of these cytokines by antigen-presenting cells (apcs), and as such provides a cytokine milieu that favors t(h)17 cell induction. in this situation, apcs are activated in response to ligands de ... | 2010 | 20958317 |
| citrobacter rodentium infection induces myd88-dependent formation of ubiquitinated protein aggregates in the intestinal epithelium. | citrobacter rodentium utilizes a type 3 secretion system (t3ss) to inject effector proteins into host intestinal epithelial cells, causing structural and functional changes in these cells during infection. here, we examined the effects of c. rodentium infection on host cell protein ubiquitination in vivo. we observed the appearance of ubiquitinated protein (ub(+)) aggregates in intestinal epithelial cells near the site of bacterial attachment. formation of aggregates was dependent on t3ss activi ... | 2010 | 20962508 |
| bacterial infection causes stress-induced memory dysfunction in mice. | the brain-gut axis is a key regulator of normal intestinal physiology; for example, psychological stress is linked to altered gut barrier function, development of food allergies and changes in behaviour. whether intestinal events, such as enteric bacterial infections and bacterial colonisation, exert a reciprocal effect on stress-associated behaviour is not well established. | 2010 | 20966022 |
| extremely drug-resistant citrobacter freundii isolate producing ndm-1 and other carbapenemases identified in a patient returning from india. | 2010 | 20974865 | |
| occurrence of gram-negative bacteria in hens' eggs depending on their source and storage conditions. | the aim of this study was to analyse the qualitative composition of gram-negative microbes, mainly of the family enterobacteriaceae, including pathogenic bacteria such as salmonella, in the albumens and yolks and on the shells of hens' eggs, depending on their source and on the temperature and duration of their storage. a total of 375 table eggs were studied, from a large-scale poultry farm, a small-scale poultry farm and a supermarket. each group was divided into 5 subgroups according to the te ... | 2010 | 21033566 |
| surveillance of antimicrobial susceptibility of aerobic and facultative gram-negative bacilli isolated from patients with intra-abdominal infections in china: the 2002-2009 study for monitoring antimicrobial resistance trends (smart). | the objective of this study was to investigate the distribution and susceptibility of aerobic and facultative gram-negative bacilli (gnb) isolated from patients with intra-abdominal infections (iais) in china. from 2002 to 2009, minimum inhibitory concentrations of 14 antibiotics for 3420 aerobic and facultative gnb from up to eight hospitals in six cities were determined by the broth microdilution method. enterobacteriaceae comprised 82.9% (2834/3420) of the total isolates, with escherichia col ... | 2010 | 21036547 |
| investigation of the cytotoxic capacity of some adherent opportunistic enterobacterial strains by the mtt assay and transmission electron microscopy. | the purpose of this study was to determine the cytotoxic effect on caco-2 intestinal cells of dialysates obtained from bacterial cultures of some enterobacterial opportunistic strains with different sources of isolation (food, stool culture, acute diarrhoea, urine culture), previously tested and selected for their intensive adherence and invasion capacity to the cellular substratum and also for their cytotoxic effect on cell monolayers. in this study the level of cytotoxicity was measured quanti ... | 2010 | 21053782 |
| bacterial decolorization of black liquor in axenic and mixed condition and characterization of metabolites. | the pulping byproducts (black liquor) cause serious environmental problem due to its high pollution load. in order to search the degradability of black liquor, the potential bacterial strains citrobacter freundii (fj581026) and citrobacter sp. (fj581023) were applied in axenic and mixed condition. results revealed that the mixed bacterial culture are more effective than axenic condition and can reduce 82% cod, 79% aox, 79% color and 60% lignin after 144 h of incubation period. additionally, the ... | 2010 | 21061144 |
| salkowski's reagent test as a primary screening index for functionalities of rhizobacteria isolated from wild dipterocarp saplings growing naturally on medium-strongly acidic tropical peat soil. | rhizobacteria isolated from wild dipterocarp saplings in central kalimantan, indonesia, were subjected to salkowski's reagent test, which is often used in detecting indolic substances. among 69 isolates grown in a low-nitrogen medium supplemented with l-tryptophan (trp), culture fluids of 29 strains were positive to the test, in which 17 bacteria turned red and other 10 pink. all the red type rhizobacteria actively converted trp into tryptophol (tol), while some yielded indole-3-acetic acid (iaa ... | 2010 | 21071871 |
| companion animals: a relevant source of extended-spectrum β-lactamase-producing fluoroquinolone-resistant citrobacter freundii. | 2010 | 21074371 | |
| rmtd2, a new allele of a 16s rrna methylase gene, has been present in enterobacteriaceae isolates from argentina for more than a decade. | the first allele of a 16s rrna methyltransferase gene, rmtd2, conferring very high resistance to all clinically available aminoglycosides, was detected in 7/1,064 enterobacteria collected in 2007. rmtd2 was located on a conjugative plasmid in a tn2670-like element inside a structure similar to that of rmtd1 but probably having an independent assembly. rmtd2 has been found since 1996 to 1998 mainly in enterobacter and citrobacter isolates, suggesting a possible reservoir in these genera. this pre ... | 2010 | 21078935 |
| characterisation of pdus, the pdu metabolosome corrin reductase, and evidence of substructural organisation within the bacterial microcompartment. | pdus is a corrin reductase and is required for the reactivation of the cobalamin-dependent diol dehydratase. it is one component encoded within the large propanediol utilisation (pdu) operon, which is responsible for the catabolism of 1,2-propanediol within a self-assembled proteinaceous bacterial microcompartment. the enzyme is responsible for the reactivation of the cobalamin coenzyme required by the diol dehydratase. the gene for the cobalamin reductase from citrobacter freundii (pdus) has be ... | 2010 | 21103360 |
| tumor suppressor foxo3 mediates signals from the egf receptor to regulate proliferation of colonic cells. | epithelial proliferation, critical for homeostasis, healing, and colon cancer progression, is in part controlled by epidermal growth factor receptor (egfr). proliferation of colonic epithelia can be induced by citrobacter rodentium infection, and we have demonstrated that activity of tumor suppressor foxo3 was attenuated after this infection. thus the aim of this study was to determine the contribution of foxo3 in egfr-dependent proliferation of intestinal epithelia and colon cancer cell lines. ... | 2010 | 21109589 |
| predation of human pathogens by the predatory bacteria micavibrio aeruginosavorus and bdellovibrio bacteriovorus. | the focus of this study was to evaluate the potential use of the predatory bacteria bdellovibrio bacteriovorus and micavibrio aeruginosavorus to control the pathogens associated with human infection. | 2010 | 21114596 |
| vi-crm 197 as a new conjugate vaccine against salmonella typhi. | an efficacious, low cost vaccine against typhoid fever, especially for young children, would make a major impact on disease burden in developing countries. the virulence capsular polysaccharide of salmonella typhi (vi) coupled to recombinant mutant pseudomonas aeruginosa exoprotein a (vi-repa) has been shown to be highly efficacious. we investigated the use of carrier proteins included in infant vaccines, standardized the conjugation process and developed key assays required for routine lot rele ... | 2010 | 21115057 |
| the apolipoprotein e-mimetic peptide cog112 inhibits nf-kappab signaling, proinflammatory cytokine expression, and disease activity in murine models of colitis. | inflammatory bowel disease (ibd), consisting of crohn's disease and ulcerative colitis, is a source of substantial morbidity and remains difficult to treat. new strategies for beneficial anti-inflammatory therapies would be highly desirable. apolipoprotein (apo) e has immunomodulatory effects and synthetically derived apoe-mimetic peptides are beneficial in models of sepsis and neuroinflammation. we have reported that the antennapedia-linked apoe-mimetic peptide cog112 inhibits the inflammatory ... | 2010 | 21115487 |
| laboratory detection of extended-spectrum-beta-lactamase-producing enterobacteriaceae: evaluation of two screening agar plates and two confirmation techniques. | the worldwide prevalence of extended-spectrum-beta-lactamase-producing esbl-producing enterobacteriaceae (esbl-e) is increasing, making the need for optimized detection techniques more urgent. in this study we investigated the performance of two esbl-e screening and two esbl-e confirmation techniques. in accordance with the dutch national guidelines (www.wip.nl), a collection of 642 highly resistant enterobacteriaceae strains, as identified by vitek2, was used to test the performances of two scr ... | 2010 | 21123527 |
| effect of transgenic rhizobacteria overexpressing citrobacter braakii appa on phytate-p availability to mung bean plants. | rhizosphere microorganisms possessing phytase activity are considered important for rendering phytate-p available to plants. in the present study, citrobacter braakii phytase gene (appa) was over-expressed in rhizobacteria possessing plant growth promoting (pgp) traits for increasing their potential as bioinoculants. appa was cloned under the lac promoter in the broad host-range expression vector pbbr1mcs2. transformation of the recombinant construct pcbappa resulted in high constitutive phytase ... | 2010 | 21124052 |
| recurrent lower limb abscesses as the primary manifestation of aorto-femoral graft paraprosthetic fistula. | a 62-year-old man was presented to our facility with recurrent right lower limb abscesses. he had an aortobifemoral graft for an aortoiliac occlusive disease. the diagnosis of paraprosthetic fistula was confirmed by performing a high-resolution contrast-enhanced computed tomography, whereas labeled leukocyte imaging provided a negative result. the graft present in the right limb was removed and extra-anatomical femoro-femoral bypass was performed along with segmental ileal resection of the affec ... | 2010 | 21126852 |
| a small-molecule inhibitor of the bacterial type iii secretion system protects against in vivo infection with citrobacter rodentium. | the type iii secretion system (t3ss) is highly conserved in many gram-negative pathogenic bacteria and functions as an injector of bacterial proteins (effectors) into host cells. t3sss are involved in establishing disease processes, but this machinery is not essential for bacterial growth or homeostasis. thus, t3ss is expected to be a candidate therapeutic target, and inhibitors of t3sss could potentially reduce virulence without causing bacterial death, thereby avoiding any subsequent developme ... | 2010 | 21139624 |
| novel genetic environment of the plasmid-mediated kpc-3 gene detected in escherichia coli and citrobacter freundii isolates from china. | the imipenem and meropenem-resistant strains citrobacter freundii hs70 and escherichia coli hs510 were isolated from patients in shanghai, china. by isoelectric focusing, pcr amplification and sequencing, these strains were each found to produce four β-lactamases: tem-1, kpc-3, shv-7 and ctx-m-14. a conjugation experiment and plasmid restriction digestion revealed that the bla (kpc-3) gene was located on the same plasmid in both isolates. bidirectional primer walking sequencing showed that the n ... | 2010 | 21153909 |
| kinetic and spectral parameters of interaction of citrobacter freundii methionine γ-lyase with amino acids. | kinetic parameters of citrobacter freundii methionine γ-lyase were determined with substrates in γ-elimination reactions as well as the inhibition of the enzyme in the γ-elimination of l-methionine by amino acids with different structure. the data indicate an important contribution of the sulfur atom and methylene groups to the efficiency of binding of substrates and inhibitors. the rate constants of the enzyme-catalyzed exchange of c-α- and c-β-protons with deuterium were determined, as well as ... | 2010 | 21166645 |
| structures of the o-polysaccharides of salmonella enterica o59 and escherichia coli o15. | the o-polysaccharide of salmonella enterica o59 was studied using sugar analysis and 2d (1)h and (13)c nmr spectroscopy, and the following structure of the tetrasaccharide repeating unit was established: →2)-β-d-galp-(1→3)-α-d-glcpnac-(1→4)-α-l-rhap-(1→3)-β-d-glcpnac-(1→ accordingly, the o-antigen gene cluster of s. enterica o59 includes all genes necessary for the synthesis of this o-polysaccharide. earlier, another structure has been reported for the o-polysaccharide of salmonella arizonae (s. ... | 2010 | 21167479 |
| citrobacter koseri as a cause of early periprosthetic infection after primary total hip arthroplasty. | periprosthetic joint infection in the acute setting is usually caused by gram-positive species and remains a major problem facing total joint surgeons. we report a case of a 53-year-old male who presented with drainage 3 weeks after primary total hip arthroplasty. citrobacter koseri was cultured from an infected hematoma in his deep tissues. surgical treatment included irrigation and debridement with femoral head and liner exchange. he received a 6-week course of ertapenem and is currently asymp ... | 2010 | 21167674 |
| thoracic empyema after laparoscopic cholecystectomy: an unusual cause. | thoracic empyema after laparoscopic cholecystectomy is a rare complication. it is associated with dropped gallstones during the operation. in this case, we report a hepatocellular adenoma hemorrhage underneath an old diaphragm rupture, causing empyema after laparoscopic cholecystectomy. | 2010 | 21170636 |
| recovery of cronobacter sakazakii from environmental surface swabbing materials using a 5-h enrichment procedure. | this study aimed to reduce the time taken to detect low numbers of cronobacter sakazakii inoculated onto environmental swabs (100, 10 or 1 cfu per swab) using a simple plating procedure for application in a dairy testing laboratory. three types of environmental swabs (biolab flexiswab, gauze swabs and the whatman swabcheck polywipe sponge) were inoculated with either cron. sakazakii in single culture or cron. sakazakii together with citrobacter freundii. a 5-h enrichment procedure of swabs in cr ... | 2010 | 20529408 |
| epithelial p38alpha controls immune cell recruitment in the colonic mucosa. | intestinal epithelial cells (iecs) compose the first barrier against microorganisms in the gastrointestinal tract. although the nf-kappab pathway in iecs was recently shown to be essential for epithelial integrity and intestinal immune homeostasis, the roles of other inflammatory signaling pathways in immune responses in iecs are still largely unknown. here we show that p38alpha in iecs is critical for chemokine expression, subsequent immune cell recruitment into the intestinal mucosa, and clear ... | 2010 | 20532209 |