Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| community respiratory viral infection in adult lung transplant recipients. | to define the epidemiology, clinical manifestations, and long-term complications of respiratory viral infections in adult lung transplant recipients. | 1998 | 9554629 |
| respiratory syncytial virus. understanding this pediatric threat. | 1998 | 9555284 | |
| rsv, recurrent wheezing, and ribavirin. | 1998 | 9556004 | |
| reduced long-term respiratory morbidity after treatment of respiratory syncytial virus bronchiolitis with ribavirin in previously healthy infants: a preliminary report. | previously healthy infants less than 6 months of age with severe respiratory syncytial virus bronchiolitis who required hospitalization were identified from hospital records. infants had been treated either conservatively (control group, n = 19) or with ribavirin added to conservative management (study group, n = 22). all infants underwent a 1-year follow-up after the initial illness. there was a significant reduction in the prevalence of reactive airway disease in the group treated with ribavir ... | 1998 | 9556006 |
| detection of respiratory syncytial virus rna in blood of neonates by polymerase chain reaction. | during the winter season of 1994/1995, nasopharyngeal aspirates and blood samples of neonates who were admitted to the neonatal intensive care unit (nicu) (group 1) and infants with respiratory tract disease (group 2) were examined prospectively for the presence of respiratory syncytial virus (rsv). examination of nasal washes were done by antigen detection and blood samples were tested by nested reverse transcription and polymerase chain reaction (rt-pcr). the results of the 41 neonates studied ... | 1998 | 9557299 |
| the class ii membrane glycoprotein g of bovine respiratory syncytial virus, expressed from a synthetic open reading frame, is incorporated into virions of recombinant bovine herpesvirus 1. | the bovine herpesvirus 1 (bhv-1) recombinants bhv-1/eg(ori) and bhv-1/eg(syn) were isolated after insertion of expression cassettes which contained either a genomic rna-derived cdna fragment (bhv-1/eg(ori)) or a modified, chemically synthesized open reading frame (orf) (bhv-1/eg(syn)), which both encode the attachment glycoprotein g of bovine respiratory syncytial virus (brsv), a class ii membrane glycoprotein. northern blot analyses and nuclear runoff transcription experiments indicated that tr ... | 1998 | 9557663 |
| recombinant vaccinia virus coexpressing the f protein of respiratory syncytial virus (rsv) and interleukin-4 (il-4) does not inhibit the development of rsv-specific memory cytotoxic t lymphocytes, whereas priming is diminished in the presence of high levels of il-2 or gamma interferon. | in order to investigate if immune responses to the fusion (f) protein of respiratory syncytial virus (rsv) could be influenced by cytokines, recombinant vaccinia viruses (rvv) carrying both the f gene of rsv and the gene for murine interleukin-2 (il-2), il-4, or gamma interferon (ifn-gamma) were constructed. in vitro characterization of rvv revealed that insertion of the cytokine gene into the vp37 locus of the vaccinia virus genome resulted in 100- to 1,000-fold higher expression than insertion ... | 1998 | 9557697 |
| recombinant respiratory syncytial virus (rsv) bearing a set of mutations from cold-passaged rsv is attenuated in chimpanzees. | a set of five missense mutations previously identified by nucleotide sequence analysis of subgroup a cold-passaged (cp) respiratory syncytial virus (rsv) has been introduced into a recombinant wild-type strain of rsv. this recombinant virus, designated ra2cp, appears to replicate less efficiently in the upper and lower respiratory tracts of seronegative chimpanzees than either biologically derived or recombinant wild-type rsv. infection with ra2cp also resulted in significantly less rhinorrhea a ... | 1998 | 9557743 |
| respiratory syncytial virus infection of human alveolar epithelial cells enhances interferon regulatory factor 1 and interleukin-1beta-converting enzyme gene expression but does not cause apoptosis. | the induction kinetics of the transcriptional activities of interferon regulatory factor 1 (irf-1), interleukin-1beta-converting enzyme (ice), and cpp32 by respiratory syncytial virus (rsv) infection of human type ii alveolar epithelial cells (a549 cells) were analyzed semiquantitatively by reverse transcriptase pcr. the appearance of ice and cpp32 protein in cell lysate was examined by western blotting analysis. the induction of apoptosis by rsv infection was examined by the appearance of dna f ... | 1998 | 9557748 |
| [epidemiology of paget's disease. peculiarities in the province of zamora]. | some paramyxovirus (measles, respiratory syncytial virus, and dog's distemper virus) are currently considered to be responsible for paget's disease of the bone. a relevant role is also given to inheritance as predisposing factor. some authors have found an association between hla antigens with this disease, but without unanimous agreement. although this hypothesis of an interaction between a genetic factor and a viral infectious agent is the most accepted universally, there is not yet a definiti ... | 1998 | 9558919 |
| a number-needed-to-treat analysis of the use of respiratory syncytial virus immune globulin to prevent hospitalization. | to estimate how many infants in selected high-risk subgroups would require treatment with respiratory syncytial virus immune globulin (rsv-ig) to avoid 1 hospital admission and to determine whether this is economically justified. | 1998 | 9559712 |
| cohorting of infants with respiratory syncytial virus. | during two winter periods (1994-1995 and 1995-1996), nasopharyngeal aspirates were obtained from infants and young children with an acute respiratory illness, after initial assessment in an area with six cubicles which serves as an admissions unit. aspirates were sent for rapid diagnostic testing. respiratory syncytial virus (rsv) positive patients were cohorted into two six-bedded bays on the paediatric wards. over the two successive winter periods studied, 347 rsv positive patients were assign ... | 1998 | 9561471 |
| acute respiratory infections: the forgotten pandemic. communiqué from the international conference on acute respiratory infections, held in canberra, australia, 7-10 july 1997. | acute respiratory infections kill 4 million children every year in developing countries, and most of these deaths are caused by pneumonia. this huge loss of life goes virtually unnoticed, despite the fact that we have two very effective ways of preventing many of the deaths from pneumonia: haemophilus influenzae type b vaccine, and standardised antibiotic treatment regimens. although h. influenzae type b vaccine has virtually eliminated diseases caused by this organism in children in developed c ... | 1998 | 9562104 |
| detection of intrauterine viral infection using the polymerase chain reaction. | intrauterine viral infection commonly presents as nonimmune hydrops fetalis or intrauterine growth restriction. cytomegalovirus (cmv) and parvovirus are commonly recognized causes of fetal infection using serology and cultures. we used the polymerase chain reaction (pcr) to evaluate the frequency of fetal viral infection and the associated clinical course and outcome. specimens (amniotic fluid, fetal blood, pleural fluid, tissue) from 303 abnormal pregnancies at risk for viral infection and 154 ... | 1998 | 9562961 |
| structural homology of the central conserved region of the attachment protein g of respiratory syncytial virus with the fourth subdomain of 55-kda tumor necrosis factor receptor. | the attachment protein g of respiratory syncytial virus (rsv) has a modular architecture. the ectodomain of the protein comprises a small folded conserved region which is bounded by two mucin-like regions. in this study, a sequence and structural homology is described between this central conserved region of rsv-g and the fourth subdomain of the 55-kda tumor necrosis factor receptor (tnfr). the three-dimensional structures of rsv-g and human tnfr were previously determined with nmr spectroscopy ... | 1998 | 9568029 |
| cell-specific expression of rantes, mcp-1, and mip-1alpha by lower airway epithelial cells and eosinophils infected with respiratory syncytial virus. | respiratory syncytial virus (rsv) is the major cause of acute bronchiolitis in infancy, a syndrome characterized by wheezing, respiratory distress, and the pathologic findings of peribronchial mononuclear cell infiltration and release of inflammatory mediators by basophil and eosinophil leukocytes. composition and activation of this cellular response are thought to rely on the discrete target cell selectivity of c-c chemokines. we demonstrate that infection in vitro of human epithelial cells of ... | 1998 | 9573240 |
| the major component of ikappabalpha proteolysis occurs independently of the proteasome pathway in respiratory syncytial virus-infected pulmonary epithelial cells. | previously we showed that infection of human type ii airway epithelial (a549) cells with purified respiratory syncytial virus (prsv) induced interleukin-8 transcription by a mechanism involving cytokine-inducible cytoplasmic-nuclear translocation of the rela transcription factor. in unstimulated cells, rela is tethered in the cytoplasm by association with the ikappab inhibitor and can be released only following ikappab degradation. in this study, we examined the spectrum of ikappab isoform expre ... | 1998 | 9573251 |
| simultaneous detection and identification of human parainfluenza viruses 1, 2, and 3 from clinical samples by multiplex pcr. | reverse transcription (rt)-pcr assays have been widely described for use in the diagnosis of human parainfluenza viruses (hpivs) and other respiratory virus pathogens. however, these assays are mostly monospecific, requiring separate amplifications for each hpiv type. in the present work, we describe multiplex rt-pcr assays that detect and differentiate hpiv serotypes 1, 2, and 3 in a combined reaction. specifically, a mixture of three pairs of primers to conserved regions of the hemagglutinin-n ... | 1998 | 9574711 |
| sustaining membership lecture award. dogma, the shade that blinds. | at the heart of medical science is the responsibility for investigators and practitioners to use the scientific method to seek and apply new knowledge to better understand the mechanisms, diagnosis, treatment, and prevention of disease. at times it is difficult to differentiate hypothesis or speculation from documented fact. this essay describes an episode in human biology and medicine in which authoritative speculation evolved into dogma and impeded investigators' proper interpretation of data ... | 1998 | 9575775 |
| concomitant viral and bordetella pertussis infections in infants. | 1998 | 9576398 | |
| risk factors for respiratory syncytial virus associated apnoea. | respiratory syncytial virus (rsv) infections are characterized by upper or lower respiratory tract symptoms including bronchiolitis and pneumonia. apnoea may be the first sign of disease in children with rsv infection. the aims of this study were the identification of independent risk factors for rsv associated apnoea and the prediction of the risk for mechanical ventilation in children with rsv associated apnoea. medical records of children younger than 12 months of age admitted with rsv infect ... | 1998 | 9578972 |
| [acute respiratory infections in pediatric intensive care units. a multicenter prospective study]. | the purpose of this study was to know the etiology, clinical background, treatment an evolution of severe infectious diseases in children admitted to pediatric intensive care units (picus). | 1998 | 9580512 |
| inactivation of human immunodeficiency virus type 1, hepatitis a virus, respiratory syncytial virus, vaccinia virus, herpes simplex virus type 1, and poliovirus type 2 by hydrogen peroxide gas plasma sterilization. | studies were conducted to determine the capability of a hydrogen peroxide gas plasma sterilization process to inactivate several types of viruses. six test agents were used: hiv type 1, human hepatitis a virus, respiratory syncytial virus, vaccinia, herpes simplex virus type 1, and poliovirus type 2. | 1998 | 9584802 |
| prevalence of antibodies to bovine virus diarrhoea virus and other viruses in bulk tank milk in england and wales. | bulk tank milk samples from 1070 dairy herds in england and wales were tested by elisa for antibodies to bovine virus diarrhoea virus (bvdv). a subset of 341 herds was tested by elisa for antibodies to bovine herpesvirus 1 (bhv-1), bovine respiratory syncytial virus (brsv) and bovine coronavirus (bcv). none of the herds had less than 40 dairy cows and none had been vaccinated against bvdv. the prevalence of bvdv antibody-positive herds in the national population was estimated at 95 per cent and ... | 1998 | 9586130 |
| detection of respiratory syncytial virus a and b and parainfluenzavirus 3 sequences in respiratory tracts of infants by a single pcr with primers targeted to the l-polymerase gene and differential hybridization. | a reverse transcription-pcr and hybridization-enzyme immunoassay (rt-pcr-eia) has been developed to identify the major agents of bronchiolitis in infants: respiratory syncytial viruses a and b (rsva and rsvb) and parainfluenzavirus 3 (piv3). two primer sets (p1-p2 and p1-p3) were selected in a conserved region of the polymerase l gene. in infected cell cultures, this method detected rsva (n = 14), rsvb (n = 13), and piv3 (n = 13), with the exclusion of piv1 (n = 4), piv2 (n = 3), measles virus ( ... | 1998 | 9508315 |
| respiratory syncytial virus infection in adult bmt recipients: effective therapy with short duration nebulised ribavirin. | respiratory syncytial virus (rsv), a common cause of both upper and lower respiratory tract infection (lrti) in infants and children, is rarely described as an infective agent in adults. it has been reported in bone marrow transplant (bmt) recipients and patients with malignancy immunosuppressed by chemotherapy. such reports are often associated with a high mortality. we report an outbreak of rsv infection which occurred predominantly in bmt recipients in which early investigation and institutio ... | 1998 | 9509980 |
| peds nurses prepare for rsv season. | 1998 | 9511716 | |
| respiratory syncytial virus-associated infections in adult recipients of solid organ transplants. | although respiratory syncytial virus (rsv) infection is known to cause severe pulmonary infections in bone marrow transplant recipients, less is known concerning its clinical course, diagnosis, and treatment in solid organ transplant recipients. | 1998 | 9513859 |
| antiviral drugs in healthy children. | several antiviral agents are available to treat viral illnesses in healthy children. in some children, treatment with acyclovir is an alternative to vaccination for the treatment and prevention of chickenpox. acyclovir also can be useful in the treatment or prevention of herpes simplex infections in neonates. ribavirin, once recommended as routine therapy for high-risk infants with respiratory syncytial virus disease, is now reserved for use in selected children. amantadine and rimantidine are e ... | 1998 | 9518953 |
| monoclonal antibody neutralization escape mutants of respiratory syncytial virus with unique alterations in the attachment (g) protein. | five monoclonal antibody (mab) neutralization escape mutants of respiratory syncytial virus (rsv) were produced by growing the long strain rsv (group a virus) in the presence of a neutralizing, group cross-reactive mab specific for the attachment protein (g). four viruses (rsv-2, -6, -14 and -15) had amino acid replacements clustered within a highly conserved centrally located 13 amino acid region (position 164-176). reactivity with group a-specific mabs and with polyclonal anti-g serum was main ... | 1998 | 9519826 |
| acute otitis media in children with bronchiolitis. | we investigated the prevalence and the etiology of acute otitis media (aom) in children with bronchiolitis to determine whether aom in such children is due entirely or mainly to respiratory syncytial virus (rsv), in which case routine antimicrobial treatment would not be appropriate. | 1998 | 9521943 |
| respiratory syncytial virus immunoglobulin and monoclonal antibodies in the prevention and treatment of respiratory syncytial virus infection. | respiratory syncytial virus (rsv) infection is particularly severe in infants with bronchopulmonary dysplasia (bpd) and in premature infants without bpd. attempts to develop a vaccine against rsv have been unsuccessful. passive immunoprophylaxis of premature infants with or without bpd using a hyperimmune human globulin against rsv (rsvig) decreases the severity of rsv infection such that the rate of hospitalization following infection is reduced by 40%. the severity of illness among hospitalize ... | 1998 | 9523402 |
| priming with secreted glycoprotein g of respiratory syncytial virus (rsv) augments interleukin-5 production and tissue eosinophilia after rsv challenge. | the respiratory syncytial virus (rsv) g glycoprotein promotes differentiation of type 2 cd4+ t lymphocytes and induces an eosinophilic response in lungs of rsv-infected mice. a unique feature of g is that a second initiation codon in the transmembrane region of the glycoprotein results in secretion of soluble protein from infected cells. recombinant vaccinia viruses that express wild-type g (vvwt g), only secreted g (vvm48), or only membrane-anchored g (vvm48i) were used to define the influence ... | 1998 | 9525607 |
| respiratory syncytial virus affects pulmonary function in balb/c mice. | balb/c mice inoculated intranasally with respiratory syncytial virus (rsv) were studied in a whole-body plethysmograph to determine if signs of respiratory illness similar to those observed in human infants could be detected. also, responsiveness to methacholine was assessed. rsv-infected mice showed significantly higher respiratory rates than did controls (409.2 vs. 305.2 breaths/min, p < .0001). significantly increased airway responsiveness to methacholine was noted, infected mice responding t ... | 1998 | 9466511 |
| relationship of serum antibody to risk of respiratory syncytial virus infection in elderly adults. | the relationship of serum antibody to respiratory syncytial virus (rsv) and risk of rsv infection was prospectively evaluated in frail elderly persons. baseline blood samples from 22 subjects who developed symptomatic rsv infection during the 26-month study and from 22 control subjects who did not become infected with rsv were compared. the mean serum igg titer to rsv fusion protein was significantly lower in the rsv-infected group than in the controls (15.4 +/- 1.6 vs. 16.4 +/- 1.8, p = .05), a ... | 1998 | 9466538 |
| safety and immunogenicity of a purified f protein respiratory syncytial virus (pfp-2) vaccine in seropositive children with bronchopulmonary dysplasia. | respiratory syncytial virus (rsv) causes serious respiratory illness in preterm children with bronchopulmonary dysplasia. in a prospective randomized placebo-controlled trial, 21 children received one dose of pfp-2 (purified fusion [f] protein) vaccine or influenza vaccine (placebo). children were followed for adverse reactions and rsv illness over two respiratory seasons. sera were obtained for determination of igg titers to rsv f protein and neutralizing antibody titers before and 1, 6, and 12 ... | 1998 | 9466539 |
| viruses and bacteria in the etiology of the common cold. | two hundred young adults with common colds were studied during a 10-month period. virus culture, antigen detection, pcr, and serology with paired samples were used to identify the infection. viral etiology was established for 138 of the 200 patients (69%). rhinoviruses were detected in 105 patients, coronavirus oc43 or 229e infection was detected in 17, influenza a or b virus was detected in 12, and single infections with parainfluenza virus, respiratory syncytial virus, adenovirus, and enterovi ... | 1998 | 9466772 |
| respiratory syncytial virus pneumonitis-induced diffuse alveolar damage in an autologous bone marrow transplant recipient. | a 24-year-old woman with recurrent hodgkin's lymphoma, stage iib nodular sclerosing type, underwent an autologous bone marrow transplantation. forty-five days after transplantation, an upper respiratory tract infection developed that progressed to respiratory distress necessitating mechanical ventilation. an open-lung biopsy demonstrated diffuse alveolar damage. after an extensive search for the cause of the respiratory compromise, we detected respiratory syncytial virus in a bronchoalveolar lav ... | 1998 | 9472999 |
| respiratory syncytial virus. | 1998 | 9473944 | |
| respiratory syncytial virus immune globulin intravenous (human) receives marketing approval in canada. | 1998 | 9481469 | |
| [ribavirin administration by aerosol]. | this article presents the recommended procedures to administer ribavirin by means of an aerosol as followed at the maternity-childcare university hospital in vall d'hebron, barcelona. ribavirin is a wide spectrum anitviral drug applied by aerosol means against severe infections caused by the respiratory syncytial virus. said administering technique is complex and requires a considerable degree of nursing care, knowledge regarding its correct use, and hospital care. bear in mind that careless adm ... | 1998 | 9534572 |
| passive protection of gnotobiotic calves using monoclonal antibodies directed at different epitopes on the fusion protein of bovine respiratory syncytial virus. | two neutralizing, fusion-inhibiting bovine monoclonal antibodies (mabs; b4 and b13) directed at different epitopes on the fusion protein of respiratory syncytial virus (rsv) protected the lungs of gnotobiotic calves from rsv infection. the mabs were administered intratracheally 24 h before the calves were challenged with bovine rsv. a third, nonneutralizing, non-fusion-inhibiting but complement-fixing mab, b1, provided no significant protection against infection, and the disease was not exacerba ... | 1998 | 9534958 |
| isolation of a second recombinant human respiratory syncytial virus monoclonal antibody fragment (fab rsvf2-5) that exhibits therapeutic efficacy in vivo. | a second human respiratory syncytial virus (rsv)-neutralizing monoclonal antibody was isolated and its binding site was identified. fab f2-5 is a broadly reactive fusion (f) protein-specific recombinant fab generated by antigen selection from a random combinatorial library displayed on the surface of filamentous phage. in an in vitro plaque-reduction test, the fab rsvf2-5 neutralized the infectivity of a variety of field isolates representing viruses of both rsv subgroups a and b. the fab recogn ... | 1998 | 9534985 |
| sequential annual administration of purified fusion protein vaccine against respiratory syncytial virus in children with cystic fibrosis. | we recently showed the clinical benefit of the pfp-2 vaccine for respiratory syncytial virus (rsv) for children with cystic fibrosis (cf). | 1998 | 9535249 |
| the clinical spectrum of respiratory syncytial virus disease in the gambia. | respiratory syncytial virus (rsv) is a well-recognized cause of lower respiratory tract infections in early childhood in industrialized countries, but less is known about rsv infection in developing countries. | 1998 | 9535250 |
| international variation in the management of infants hospitalized with respiratory syncytial virus. international rsv study group. | respiratory syncytial virus (rsv) is a frequent cause of hospitalization among infants. to compare patient management in europe, the united states, and australia, we analyzed the charts of 1,563 pediatric patients hospitalized with laboratory-confirmed rsv lower respiratory infections during recent rsv seasons. half of patients had been seen initially as outpatients. median duration of hospitalization was 4 days in australia, finland, the united kingdom, and the united states, and 8 or 9 days in ... | 1998 | 9537488 |
| expression of the neutrophil chemoattractant interleukin-8 in the lesions of bovine pneumonic pasteurellosis. | we investigated the expression of interleukin-8 (il-8) in pneumonic pasteurellosis of cattle because neutrophils are important mediators of tissue injury in this disease and because il-8 is a major neutrophil chemoattractant in other species. we also compared il-8 expression in bacterial and viral pneumonia, since the latter lacks the severe neutrophil exudation typical of pneumonic pasteurellosis. il-8 expression was assessed by northern analysis, in situ hybridization, enzyme-linked immunosorb ... | 1998 | 9539366 |
| identification of a conserved neutralization site in the first heptad repeat of the fusion protein of respiratory syncytial virus. | a large set of monoclonal antibodies (mabs) directed against the fusion glycoprotein complex f1f2 of bovine respiratory syncytial virus (brsv) and several polyclonal sera from infected or vaccinated animals were tested in pepscan to locate linear epitopes on the f-protein. the polyclonal sera mapped to antigenic sites that correspond exactly to known antigenic sites on the f protein of human rsv. only the neutralizing mab 3 could be mapped with pepscan. mab 3 reacted with three successive overla ... | 1998 | 9541615 |
| baculovirus expression of the fusion protein gene of bovine respiratory syncytial virus and utility of the recombinant protein in a diagnostic enzyme immunoassay. | the fusion (f) protein of bovine respiratory syncytial virus (brsv) was expressed by using a baculovirus vector. antigenicity was tested by immunofluorescence analysis with f-specific monoclonal and polyclonal antibodies. antibodies to recombinant f protein raised in a rabbit neutralized brsv and human respiratory syncytial virus infectivity when tested in a plaque reduction assay. the recombinant f protein was evaluated as a source of antigen in an enzyme-linked immunosorbent assay (elisa), and ... | 1998 | 9542947 |
| impact of influenza and other community-acquired viruses. | the incidence of community-acquired pneumonia peaks during the winter season each year. increases in mortality from these pneumonias and influenza-like illnesses (p&i) above an "epidemic threshold" for 2 or more weeks generally signify increased numbers of influenza virus infections in the community, although peaks in p&i mortality typically lag a few weeks behind peaks in influenza virus activity. most of the pneumonias cases found during influenza virus epidemics are due to secondary bacterial ... | 1998 | 9543472 |
| a peptide mimic of a protective epitope of respiratory syncytial virus selected from a combinatorial library induces virus-neutralizing antibodies and reduces viral load in vivo. | respiratory syncytial virus (rsv) is the most important cause of bronchiolitis and pneumonia in infants and young children worldwide. as yet, there is no effective vaccine against rsv infection, and previous attempts to develop a formalin-inactivated vaccine resulted in exacerbated disease in recipients subsequently exposed to the virus. in the work described here, a combinatorial solid-phase peptide library was screened with a protective monoclonal antibody (mab 19) to identify peptide mimics ( ... | 1998 | 9499058 |
| autocrine regulation and experimental modulation of interleukin-6 expression by human pulmonary epithelial cells infected with respiratory syncytial virus. | the mechanisms of regulation of interleukin-6 (il-6) production in respiratory syncytial virus (rsv)-infected respiratory epithelial cells were evaluated in a549 cell cultures. incubation with purified rsv resulted in significant production of il-1alpha, il-1beta, il-6, and tumor necrosis factor alpha (tnf-alpha). addition of saturating concentrations of neutralizing antibodies against il-1alpha, il-1beta, or tnf-alpha into purified rsv-infected cell cultures resulted in a significant inhibition ... | 1998 | 9499112 |
| safety, tolerance and pharmacokinetics of a humanized monoclonal antibody to respiratory syncytial virus in premature infants and infants with bronchopulmonary dysplasia. medi-493 study group. | respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract infection in infants. medi-493 (palivizumab) is a humanized monoclonal antibody to the fusion protein of rsv and is active in animal models for prevention of pulmonary rsv replication. | 1998 | 9493805 |
| evaluation of a single dilution elisa system for detection of seroconversion to bovine viral diarrhea virus, bovine respiratory syncytial virus, parainfluenza-3 virus, and infectious bovine rhinotracheitis virus: comparison with testing by virus neutralization and hemagglutination inhibition. | a single-dilution quantitative enzyme-linked immunosorbent assay (elisa) system, based on commercial elisa kits, for the simultaneous detection of seroconversion to bovine viral diarrhea virus (bvdv), bovine respiratory syncytial virus (brsv), parainfluenza-3 virus (pi3v), and infectious bovine rhinotracheitis virus (ibrv) was evaluated by testing acute and convalescent serum pairs from 564 cattle in 145 outbreaks of respiratory disease. seroconversion to bvdv, brsv, pi3v and ibrv was detected i ... | 1998 | 9526859 |
| lower airway disease caused by respiratory syncytial virus. | respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract disease in infants and young children. most infections due to rsv are mild and do not require hospitalization. rsv causes both upper respiratory tract infections as well as lower respiratory tract infections. infants with underlying disease states like bronchopulmonary dyslasia, congenital heart disease and prematurity appear more prone to develop severe infection and have a higher incidence of hospitalization. ... | 1998 | 10771985 |
| subgroup determination of respiratory syncytial virus by rt-pcr. | pcr optimization for differentiation of rsv subgroup a (rt-pcr-1) and rsv subgroup b (rt-pcr-2) were developed. various conditions of rt-pcr-1 and rt-pcr-2 were summarized. these methods are highly specific and sensitive to differentiate rsv subgroup a and rsv subgroup b from the other respiratory viruses. | 1998 | 10772559 |
| anaphylactic sensitization to aeroantigen during respiratory virus infection. | virus infections frequently exacerbate asthma, and in some cases may even precipitate its onset. although this association is well known, experimental investigation has been hampered by the lack of adequate models. | 1998 | 10024221 |
| rsv-associated hospitalizations in alaska native infants. | retrospective reviews for 1986-1992 suggested that alaska native children experience high rates of respiratory syncytial virus (rsv)-associated hospitalization; however, the epidemiology of rsv infections has been poorly characterized. | 1998 | 10093285 |
| an infection out of season: respiratory syncytial virus. | 1998 | 10095672 | |
| [rhinovirus infections in hospitalized children: a 3-year study]. | rhinoviruses (rh) are responsible for acute respiratory illnesses, mainly in the upper respiratory tract. | 1998 | 10223104 |
| [respiratory syncytial virus infections and ribavirin: the controversy continues]. | 1998 | 10223129 | |
| [is the use of ribavirin aerosols in respiratory syncytial virus infections justified? clinical and economic evaluation]. | the controversy about the use of ribavirin aerosol for children at risk (cardiopathy, pneumopathy, premature and immunodeficient patients), in case of respiratory syncytial virus (rsv) infection, led us to stop its prescription in 1993 and study prospectively the patients admitted during the following winters. | 1998 | 10223131 |
| [penicillin-resistant pneumococcal pneumonia (serotype p14), and coinfection with respiratory syncytial virus and mycoplasma]. | a 3-year old child was admitted for a pneumococcal pneumonia with pleural effusion, initially treated with amoxicillin and clavulanic acid. clinical deterioration suggested a resistance to conventional antibiotics which was confirmed by bacteriological investigation. a co-infection with respiratory syncitial virus and mycoplasma pneumoniae was associated. under adapted antibiotherapy, the clinical course improved. | 1998 | 10223160 |
| -prevention of respiratory syncytial virus infection-. | respiratory syncytial virus (rsv) is responsible for annual outbreaks of severe respiratory disease among infants. its prevention has been considered for many years but the first vaccination trials resulted in diseases of increased severity. new vaccines are in progress with promising results, although their efficacy in the presence of maternal antibodies, and their tolerance in very young babies, remain to be demonstrated. concerning passive immunization, intravenous anti-rsv immunoglobulins ha ... | 1998 | 10328005 |
| [clinical and experimental studies in treating infantile acute respiratory tract infection with feiyan chuansou oral liquid]. | to study the therapeutical mechanism of feiyan chuansou oral liquid (fcol) in treating infantile acute respiratory trct infection. | 1998 | 11367653 |
| respiratory syncytial virus infection among young children with acute respiratory tract infection in iraq. | the incidence of respiratory syncytial virus infection was assessed among 516 children under 5 years with acute respiratory infection and 57 control children free of respiratory infection to determine its relation to epidemiological variables. respiratory syncytial virus was detected in 188 (37.6%) children with acute respiratory infection and in none of the control group. the infection was highest in those with severe acute respiratory infection, particularly severe bronchiolitis and pneumonia ... | 1999 | 10983533 |
| recent advances in the understanding of childhood asthma. | the worldwide increase in diagnosed cases of asthma in the last 15 years, in spite of the better understanding of the pathogenesis of the disease and the availability of more potent bronchodilators and anti-inflammatory agents, has been the concern of clinicians worldwide. for pediatricians, it is most disturbing to learn that asthma is the most common chronic disease in childhood. in addition, asthma hospitalization rates are highest among children, and death rates from asthma are among the hig ... | 1999 | 10910604 |
| respiratory syncytial virus infection of human respiratory epithelial cells enhances both muscarinic and beta2-adrenergic receptor gene expression. | the possible changes in transcriptional activities of the m1 muscarinic acetylcholine receptor (machr) and beta2-adrenergic receptor (ar) genes in respiratory syncytial virus (rsv)-infected human type 2 alveolar epithelial cells (a549 cells) were analyzed semiquantitatively by reverse transcription-polymerase chain reaction (rt-pcr). rsv enhanced m1 machr gene expression significantly at 4 hrs post infection (p.i.), and this enhancement persisted until 10 hrs, after peaking at 7 hrs. beta2-ar ge ... | 1999 | 10749375 |
| bronchiolitis in infants. | bronchiolitis is a common disease of the lower respiratory tract of infants, resulting from inflammatory obstruction of the small airways. it is a predominant viral illness, in which the rsv (respiratory syncytial virus) is the agent in more that 50% of cases. it is not known how many children with genetic predisposition to atopy develop asthma symptoms after bronchiolitis, however the relationship between the two affections is not understood. we show that a major proportion of babies with a pos ... | 1999 | 11073128 |
| pulmonary function and exercise challenge test in thai children: 10 years post respiratory syncytial virus infection. | to report the occurrence of asthma, pulmonary function and exercise challenge test abnormalities found in thai children, who 10 years earlier had lower respiratory tract infection caused by respiratory syncytial virus (rsv-lri). | 1999 | 10730535 |
| synergistic effects of concurrent challenge with bovine respiratory syncytial virus and 3-methylindole in calves. | to evaluate the potential synergy between bovine respiratory syncytial virus (brsv) and 3-methylindole (3mi) in inducing respiratory disease in cattle. | 1999 | 10328425 |
| absence of lung immunopathology following respiratory syncytial virus (rsv) challenge in mice immunized with a recombinant rsv g protein fragment. | the relative immunopathogenic potential of a recombinant fusion protein incorporating residues 130-230 of respiratory syncytial virus (rsv-a) g protein (bbg2na), formalin-inactivated rsv-a (fi-rsv), and phosphate-buffered saline (pbs) was investigated in mice after immunization and rsv challenge. fi-rsv priming resulted in massive infiltration of b cells and activated cd4(+) and cd8(+) t lymphocytes in mediastinal lymph nodes (mln) and lungs, where eosinophilia and elevated ifn-gamma, il-2, -4, ... | 1999 | 10329575 |
| clinical impact of preoperative respiratory syncytial virus testing. | the purpose of this study was to determine if preoperative assessment (i.e., physical examination and history review) of pediatric patients can predict positive test results for respiratory syncytial virus (rsv) infection, surgery cancellations, and adverse anesthetic outcomes. approximately 400 pediatric patients were identified for the study from infection control statistics and classified by rsv test result and surgery cancellation status into three groups for analyses. researchers analyzed r ... | 1999 | 10332554 |
| a novel and effective intranasal immunization strategy for respiratory syncytial virus. | in designing subunit vaccination strategies for respiratory syncytial virus (rsv), immunization by mucosal routes may present a realistic alternative to parenteral administration for inducing protective immune responses. to this end, we have utilized the balb/c mouse model and an adjuvant formulation containing caprylic/capric glycerides (ccg) and polyoxyethylene-20-sorbitan monolaurate (ps). the intranasal (i.n.) delivery of purified natural f protein (3 microg per vaccine) formulated with ccg- ... | 1999 | 10333241 |
| [broncho-alveolitis in infants. diagnosis and treatment]. | 1999 | 10337225 | |
| respiratory syncytial virus infects the bonnet monkey, macaca radiata. | the bonnet monkey model of respiratory syncytial virus (rsv) infection may be a useful nonhuman primate model for studying rsv disease in humans because bonnet monkeys can predictably be infected to obtain an orderly sequence of morphologic, cytologic, virologic, serologic, and inflammatory changes related to time of infection. young feral bonnet monkeys, macaca radiata, were infected endotracheally with 10(6) plaque-forming units (pfu) of the long strain of rsv. rsv was recovered from the anima ... | 1999 | 10347274 |
| phenylephrine hydrochloride nose drops in adjunctive treatment of respiratory syncytial virus infection. | 1999 | 10349087 | |
| [detection of respiratory syncytial virus using immunohistochemistry. report of 53 cases of sudden infant death]. | we analyzed 53 cases of sudden infant death to detect immunohistochemical expression of respiratory syncytial virus on pulmonary sections. the virus was identified in 7 cases. the immunohistochemical staining was intracytoplasmic and mainly observed in bronchioles. among these 7 cases, 6 showed severe pulmonary lesions which were assumed to be accountable for decrease. the inflammatory lesions related to respiratory syncytial virus were diffuse, located with the same intensity to either bronchi, ... | 1999 | 10349473 |
| association of radiologically ascertained pneumonia before age 3 yr with asthmalike symptoms and pulmonary function during childhood: a prospective study. | epidemiologic evidence suggests an association between reports of pneumonia in early life and the subsequent development of diminished lung function. however, no studies are available in which the diagnosis of pneumonia was based on radiologic evidence. lower respiratory illnesses with or without a radiologically confirmed diagnosis of pneumonia were assessed in a study of 888 children enrolled at birth. pulmonary function tests, markers of atopy, asthma diagnosis, and prevalence of respiratory ... | 1999 | 10351936 |
| respiratory syncytical virus-induced chemokine expression in the lower airways: eosinophil recruitment and degranulation. | characterization of chemokine expression patterns in virus-infected epithelial cells provides important clues to the pathophysiology of such infections. the aim of this study was to determine the chemokine response pattern of respiratory epithelium when infected with respiratory syncytial virus (rsv). macrophage inflammatory protein-1-alpha (mip-1-alpha), interleukin-8 (il-8), and rantes concentrations were measured from rsv-infected hep-2, mrc-5, and wi-38 cell culture supernatants daily follow ... | 1999 | 10351940 |
| analysis of ruminant respiratory syncytial virus isolates by rnase protection of the g glycoprotein transcripts. | two different respiratory syncytial virus (rsv) radiolabeled probes were used to characterize the genetic heterogeneity of 25 ruminant rsv isolates by the ribonuclease protection assay. a 32p-radiolabeled antisense rna probe was transcribed from cloned ovine and bovine rsv g glycoprotein genes and then hybridized with total rna isolated from infected cells with various ruminant rsv isolates. the results of this study, along with previously published nucleotide sequence data of the ovine rsv g gl ... | 1999 | 10353351 |
| a direct comparison of the activities of two humanized respiratory syncytial virus monoclonal antibodies: medi-493 and rshzl9. | two humanized monoclonal antibodies, medi-493 and rshz19, were developed independently as potential improvements over rsv-igiv for prevention of respiratory syncytial virus (rsv) infection. rsv-igiv is a polyclonal human antibody preparation for intravenous infusion enriched for rsv neutralizing activity. a phase iii clinical trial showed that medi-493 significantly reduced hospitalizations due to rsv infection. in a separate trial, rshz19 failed to show significant efficacy. in new studies, the ... | 1999 | 10353858 |
| severe respiratory syncytial virus disease in alaska native children. rsv alaska study group. | hospitalization rates for respiratory syncytial virus (rsv) infection range from 1 to 20/1000 infants. to determine the rate and severity of rsv infections requiring hospitalization for infants in the yukon-kuskokwim (yk) delta of alaska, a 3-year prospective surveillance study was conducted. the annual rate of rsv hospitalization for yk delta infants <1 year of age was 53-249/1000. rsv infection was the most frequent cause of infant hospitalization. rsv disease severity did not differ among non ... | 1999 | 10353859 |
| [round table: urticaria in relation to infections]. | 1) to study the clinical and analytic features of infectious disease associated to urticaria in children. 2) to look into the probable etiology of the infectious disease. 3) to determine atopic predisposition and previous urticarial episodes and to rule out the involvement of antibiotics. | 1999 | 10354011 |
| crystal structure of two cd46 domains reveals an extended measles virus-binding surface. | measles virus is a paramyxovirus which, like other members of the family such as respiratory syncytial virus, is a major cause of morbidity and mortality worldwide. the cell surface receptor for measles virus in humans is cd46, a complement cofactor. we report here the crystal structure at 3.1 a resolution of the measles virus-binding fragment of cd46. the structure reveals the architecture and spatial arrangement of two glycosylated short consensus repeats with a pronounced interdomain bend and ... | 1999 | 10357804 |
| viral infection modulates expression of hypersensitivity pneumonitis. | hypersensitivity pneumonitis (hp) is a granulomatous, inflammatory lung disease caused by inhalation of organic ags, most commonly thermophilic actinomycetes that cause farmer's lung disease. the early response to ag is an increase in neutrophils in the lung, whereas the late response is a typical th1-type granulomatous disease. many patients who develop disease report a recent viral respiratory infection. these studies were undertaken to determine whether viruses can augment the inflammatory re ... | 1999 | 10358192 |
| safety and pharmacokinetics of an intramuscular monoclonal antibody (sb 209763) against respiratory syncytial virus (rsv) in infants and young children at risk for severe rsv disease. | we conducted a multicenter, double-blind, placebo-controlled, randomized trial of a humanized monoclonal antibody against a respiratory syncytial virus (rsv) fusion protein (sb 209763) to evaluate its safety, pharmacokinetics, and fusion inhibition and neutralization titers. forty-three infants who were either delivered prematurely (</=35 weeks' gestation) or exhibited bronchopulmonary dysplasia were administered either single or repeat (two doses, 8 weeks apart) intramuscular injections of sb 2 ... | 1999 | 10223933 |
| airborne transmission of bhv1, brsv, and bvdv among cattle is possible under experimental conditions. | to control the diseases caused by bovine herpesvirus 1 (bhv1), bovine respiratory syncytial virus (brsv), and bovine virus diarrhoea virus (bvdv), it is crucial to know their modes of transmission. the purpose of this study was to determine whether these viruses can be transmitted by air to a substantial extent. calves were housed in two separate isolation stables in which a unidirectional airflow was maintained through a tube in the wall. in one stable, three of the five calves were experimenta ... | 1999 | 10227122 |
| protective efficacy against respiratory syncytial virus following murine neonatal immunization with bbg2na vaccine: influence of adjuvants and maternal antibodies. | alum-adsorbed bbg2na, a recombinant vaccine derived in part from the respiratory syncytial virus (rsv) subgroup a g protein, induced moderate antibody titers after 1 immunization in 1-week-old mice but conferred complete lung protection upon rsv challenge. the anti-bbg2na igg1-igg2a neonatal isotype profile was suggestive of dominant th2 responses compared with those in adults. formulation of bbg2na with a th1-driving adjuvant efficiently shifted neonatal responses toward a more balanced and adu ... | 1999 | 10228051 |
| upper respiratory tract infections. | in reviewing recent advances in upper respiratory tract infections, we focus on five key topics. first, the use of ribavirin in the treatment of respiratory syncytial virus infection has been limited to the immunosuppressed. prophylaxis in high-risk patients with specific immunoglobulin is effective and a new monoclonal antibody shows promise. second, the efficacy of neuraminidase inhibitors in the treatment of influenza has become established. there are unresolved concerns about early implement ... | 1999 | 10228740 |
| an in vitro selected binding protein (affibody) shows conformation-dependent recognition of the respiratory syncytial virus (rsv) g protein. | using phage-display technology, a novel binding protein (z-affibody) showing selective binding to the rsv (long strain) g protein was selected from a combinatorial library of a small alpha-helical protein domain (z), derived from staphylococcal protein a (spa). biopanning of the z-library against a recombinant fusion protein comprising amino acids 130-230 of the g protein from rsv-subgroup a, resulted in the selection of a z-affibody (z(rsv1)) which showed g protein specific binding. using biose ... | 1999 | 10231093 |
| a single amino acid substitution in the phosphoprotein of respiratory syncytial virus confers thermosensitivity in a reconstituted rna polymerase system. | the single amino acid change gly172 to ser in the phosphoprotein (p) of respiratory syncytial virus (rsv) has previously been shown to be responsible for the thermosensitivity and protein-negative phenotype of tsn19, a mutant of the b subgroup rsn-2 strain. this single change was inserted into the p gene of the a subgroup virus rss-2, and the resulting phenotype was observed in a plasmid-driven reconstituted rsv rna polymerase system. expression from a genome analogue containing two reporter gen ... | 1999 | 10233981 |
| the major attenuating mutations of the respiratory syncytial virus vaccine candidate cpts530/1009 specify temperature-sensitive defects in transcription and replication and a non-temperature-sensitive alteration in mrna termination. | the live-attenuated respiratory syncytial virus vaccine candidate cpts530/1009 was previously shown to contain two separate amino acid changes in the l protein, mutations 530 and 1009 (phe-521-->leu and met-1169-->val, respectively, according to the amino acid sequence of the l protein). each mutation independently specifies temperature-sensitive (ts) and attenuation phenotypes. in this study, we examined the effects of these mutations on transcription and rna replication, using complete infecti ... | 1999 | 10233984 |
| molecular epidemiology of respiratory syncytial virus in the gambia. | respiratory syncytial virus (rsv) infection in the gambia occurs seasonally in association with the rainy season. this study examined the genetic variability of rsv isolates from four consecutive epidemics from 1993-6. each epidemic was made up of a number of variants which were replaced in subsequent epidemics. analysis of attachment (g) protein gene sequences showed that isolates were closely related to those observed in the rest of the world. however, many isolates from 1993 and 1994 were unl ... | 1999 | 10098799 |
| the effects of bovine respiratory syncytial on normal ovine lymphocyte responses to mitogens or antigens in vitro. | in the present study peripheral blod mononuclear cells (mnc) obtained from normal uninfected lambs were used to study the possible effects of bovine respiratory syncytial virus (brsv) on lymphocyte responses to the mitogens, phytohaemagglutinin (pha), concanavalin a (con a) and pokeweed mitogen (pwm) in vitro. live brsv had a depressive effect on the proliferative responses of normal mnc to pha, con a and pwm. inactivated brsv and a commercial preparation of prostaglandin e2 were also found to d ... | 1999 | 10099024 |
| efficacy of rd3-0028 aerosol treatment against respiratory syncytial virus infection in immunosuppressed mice. | rd3-0028, a benzodithiin compound, has antiviral activity against respiratory syncytial virus (rsv) in cell culture. we used a mouse model of rsv infection to determine the in vivo effect of rd3-0028. cyclophosphamide (cyp)-treated, immunosuppressed mice were inoculated intranasally. the lungs of the mice were removed on day 4. the virus titers of the lungs of rd3-0028-treated mice were compared to the virus titers of the lungs of virus-inoculated, untreated control mice. in an effort to increas ... | 1999 | 10103176 |
| immunoprophylaxis with palivizumab, a humanized respiratory syncytial virus monoclonal antibody, for prevention of respiratory syncytial virus infection in high risk infants: a consensus opinion. | 1999 | 10093942 | |
| questions about palivizumab (synagis) | 1999 | 10026068 | |
| questions about palivizumab (synagis) | 1999 | 10026069 | |
| virus-inducible expression of a host chemokine gene relies on replication-linked mrna stabilization. | analysis of the host response to viral infection generally has focused on the capacity of viruses to activate or repress transcription of cellular genes, and this approach is also characteristic of work on rna viruses such as respiratory syncytial virus (rsv). in the present study, it appeared initially that rsv-driven expression of a critical immune regulator, the beta-chemokine rantes (regulated upon activation, normal t cell expressed and secreted), in primary-culture airway epithelial cells ... | 1999 | 10318944 |