Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| clonal expansion of human b73.1-positive natural killer cells or large granular lymphocytes exerting strong antibody-dependent and -independent cytotoxicity and occasionally lectin-dependent cytotoxicity. | b73.1-positive natural killer (nk) cells were separated from b73.1- cells by fluorescent-activated cell sorting and used for the generation of cytotoxic clones. the b73.1+ and b73.1- cells were cultured at one responder cell per well in medium with feeder cells and interleukin 2 but without lectins. the plating efficiency of b73.1- cells (about 4%) but not of b73.1+ cells was increased 3- to 7-fold by addition of lectin. all b73.1+ clones showed a high cytolytic activity against a wide variety o ... | 1983 | 6679886 |
| zollinger-ellison syndrome, prolonged cimetidine administration and nasopharyngeal carcinoma:--a coincidence? | a patient with zollinger-ellison syndrome who developed an undifferentiated nasopharyngeal carcinoma (npc) after 3 years of daily treatment with a least 1.0 g cimetidine is described. the question is raised whether large doses of cimetidine, administered for long periods to patients perhaps already immunologically compromised, may change t-suppressor cell functions so as to enable proliferation of oncogenic viruses (e.g. eb virus) and the appearance of tumor. we feel that when the population at ... | 1983 | 6323340 |
| epstein-barr virus and the x-linked lymphoproliferative syndrome. | 1983 | 6324556 | |
| problems in pediatric otorhinolaryngic pathology. iv. epithelial and lymphoid tumors of the sinonasal tract and nasopharynx. | the clinical and histopathologic diagnostic problems of childhood nasopharyngeal carcinoma, sinonasal epithelial tumors, and burkitt's lymphoma are described, and the relationship of the epstein-barr virus to tumors is discussed. | 1983 | 6327553 |
| restrictions upon epstein-barr virus infection of the leukemic cell are demonstrated in patients with hairy cell leukemia. | we tested the hypothesis that epstein-barr virus (ebv) might actually infect leukemic hairy cells in vivo by examining those cells for the ebv-receptor, ebv nuclear antigen (ebna) and membrane antigen (ma), for spontaneous transformation and rescue of infectious virus and for presence of ebv genome. ebv-receptors were found on subpopulations of leukemic cells from each of 7 patients with hairy cell leukemia (hcl) tested. ma was present on low numbers (1-5 per cent) of fresh leukemic cells of 7 p ... | 1983 | 6329936 |
| phenotypic and cytogenetic characteristics of a new epstein-barr virus negative cell line (skw 4) derived from a b-cell lymphoma. | a new epstein-barr virus nuclear antigen (ebna) negative cell line skw 4 has been established in vitro from a patient with diffuse histiocytic lymphoma. the skw 4 seems to be an authentic human tumour cell line as evidenced by its ebv negativity, monoclonality and aneuploidy tested during early in vitro passage. the cell line expresses surface mu and kappa-chains, hla-dr antigen, c3 and fc receptors and b-cell lineage antigens. the karyotypic analyses demonstrated many numerical and structural a ... | 1983 | 6329938 |
| an enzyme-linked immunosorbent assay for an antigen related to non-a, non-b hepatitis and its antibody: partial characterization of the antigen and chimpanzee transmission. | an enzyme-linked immunosorbent assay (elisa) was developed based on sera from patients convalescent from non-a, non-b hepatitis and haemophilia a patients who had been frequently treated with commercial blood products. using this elisa, an antigen was detected which appears to be related to non-a, non-b hepatitis. the antigen is provisionally designated as ds-antigen (ds-ag). the serum of another patient with haemophilia a, which was strongly positive for the ds-ag, caused a typical case of non- ... | 1983 | 6403665 |
| gamma interferon synthesis by human thymocytes and t lymphocytes inhibited by cyclosporin a. | cyclosporin a depresses the synthesis of gamma interferon by human thymocytes and t lymphocytes in vitro. this observation is of potential clinical significance because the long-term treatment of transplant patients with cyclosporin a, a widely used immunosuppressive agent, can give rise to b-cell lymphoma resulting from epstein-barr virus activation. | 1983 | 6407112 |
| a monoclonal antibody defining human b cell differentiation antigen (hlb-1 antigen). | a new monoclonal antibody specific for human b cell differentiation antigen (hlb-1) is produced by a hybridoma established by fusion of splenocytes of mice immunized with the epstein-barr virus (ebv)-transformed peripheral b cell line, rpmi-8057. this monoclonal, antibody designated anti-hlb-1 monoclonal antibody (anti-hlb-1), reacted with surface immunoglobulin (sig)-positive b cells of normal peripheral blood and lymphoid tissues and sig-positive leukemic cells. the cells of t cell leukemia, n ... | 1983 | 6408355 |
| hepatitis in patients with acute nonlymphocytic leukemia. | three consecutive groups (university of maryland cancer center protocols 7110, 7405, and 7802) of patients with acute nonlymphocytic leukemia who achieved a complete hematologic remission with similar antileukemic therapy were reviewed for the development of hepatitis. ninety-four (73 percent) experienced viral hepatitis; eight had type b hepatitis and 86 had non-a/non-b hepatitis. the hepatitis was mild in all patients. hepatitis secondary to cytomegalovirus, herpes simplex virus, epstein-barr ... | 1983 | 6412546 |
| an epidemic of non-a, non-b hepatitis in japan. | an epidemic of viral hepatitis was studied in the okitsu area of shimizu city in japan from april 1980 to november 1981. one hundred thirty-six serum samples from patients with viral hepatitis were tested for hepatitis b virus, hepatitis a virus, epstein-barr virus, and cytomegalovirus infection. immunoserological data showed the outbreak of viral hepatitis to be non-a, non-b hepatitis. epidemiological investigations revealed that the mode of the outbreak was from person to person by close conta ... | 1983 | 6414290 |
| immunologic abnormalities in patients with hemophilia a. | to assess the immunologic status of healthy persons with hemophilia a, we performed studies of t cell immunity in 21 patients, 10 given only cryoprecipitate and 11 given factor viii concentrate. patients in the factor viii group had significantly decreased helper/suppressor t cell ratios. both groups had diminished mononuclear cell response to phytohemagglutinin and normal mixed lymphocyte culture, compared with controls. abnormalities in t cell number or function did not correlate with the pres ... | 1983 | 6415259 |
| characterization of a monoclonal antibody that reacts with an activation antigen on human b cells: reactions on mitogen-stimulated blood lymphocytes and cells of normal lymph nodes. | we describe a monoclonal antibody that reacts with human b-lymphoid cells. we have characterized the reactions of this antibody on normal blood lymphocytes, with and without pokeweed mitogen stimulation, bone marrow lymphocytes, and on frozen sections of normal lymph nodes. the antibody, b532, appears to recognize an activation antigen on human b cells. this activation antigen can apparently be induced both by infection with epstein-barr virus and by stimulation with antigens and mitogens, but i ... | 1983 | 6416334 |
| prospective study of post-transfusion hepatitis after cardiac surgery in a british centre. | a series of 248 consecutive patients undergoing cardiac surgery were examined in a prospective study of post-transfusion hepatitis in a single british centre. patients received a total of 1796 units of blood or blood products (mean blood transfusion 6.28 units per patient). during five to 30 days after operation 38 of the patients showed an increase in serum transaminase activities. there was no serological evidence for fresh infection by hepatitis a or b virus, cytomegalovirus, epstein-barr vir ... | 1983 | 6416439 |
| uv light-induced immunoglobulin heavy-chain class switch in a human lymphoblastoid cell line. | during attempts to select nonsecretory variants from 0.467.3, and epstein-barr virus-transformed human lymphoblastoid cell line that secretes small amounts of igm lambda, we exposed the cells to uv light. cells that survived the irradiation were subcultured and their supernatants were screened for immunoglobulin production by an enzyme-linked immunosorbent assay (elisa). although stable nonsecretory variants were not isolated, we report here that an immunoglobulin class switch occurred in the uv ... | 1983 | 6417540 |
| antibodies to human squamous cell carcinoma. | we are studying membrane antigens of human squamous cell cancer with the use of naturally occurring autologous antibodies from patients' sera, along with a set of other serologic reagents and monoclonal antibodies raised against cultured squamous cell lines. twenty-eight squamous cell carcinoma cell lines have been established in our laboratory from tissues obtained from 23 patients. antibody reactivity has been found against the autologous tumor cell line in 13 of 23 patients. one of these is o ... | 1983 | 6417595 |
| an enzyme-linked immunosorbent assay for the detection of hepatitis non-a, non-b antigen and antibody. | an enzyme-linked immunosorbent assay (elisa) was developed, based on sera from patients convalescent from non-a, non-b hepatitis and haemophilia a patients. using this elisa an antigen (ds-ag) was detected which appears to be related to non-a, non-b hepatitis. the serum of another patient with haemophilia a, which was strongly positive for the ds-ag, caused non-a, non-b hepatitis in a chimpanzee. ds-ag could be detected in the serum of the chimpanzee during the acute phase of the infection. the ... | 1983 | 6418592 |
| hla-a, -b, and -dr antigens in north african patients with nasopharyngeal carcinoma. | seventy-six north african patients (most from algeria) affected with nasopharyngeal carcinoma (npc) have been studied for their hla-a, -b, and -dr phenotypes and compared with a control north african population. antigens hla-a3, hla-b5 and hla-bw15 were found more frequently in the npc group than in the control group (30.3% vs 17.6%, 38.2% vs 24.4% and 9.2% vs 0.8%, respectively). hla-aw33, hla-b14 and hla-dr4 were less frequent in the patients than in the controls (3.9% vs 16.8%, 1.3% vs 16% an ... | 1983 | 6419386 |
| isolation of daudi cells with reduced sensitivity to interferon. i. characterization. | treatment of daudi cells with successively increasing concentrations of interferon-alpha resulted in the selection of a cell population which multiplied in the continued presence of 10(4) units/ml of interferon-alpha. a number of clones of interferon-resistant daudi cells were isolated from this population. two clones, dif2 and dif3, were found to exhibit moderate and pronounced resistance, respectively, to both the antiviral and antiproliferative actions of human interferons-alpha and -beta. th ... | 1983 | 6420510 |
| role of t cell subpopulations in the control of the proliferative potential of ebv transformed b cells. | 1983 | 6152688 | |
| [lymphoblastoid cell lines transformed by the epstein-barr virus. cellular model in culture for the study of lysosomal enzyme disorders]. | 1983 | 6320695 | |
| [tonsillar cancer and epstein-barr virus]. | epstein-barr virus is an important aetiological factor in the development of nasopharyngeal carcinoma (npc). serology suggested that besides npc some other tumours located within the lymphoepithelial ring of the throat (waldeyer's ring) might be associated with ebv. nucleic-acid hybridizations were carried out to detect the presence of ebv-dna in tumour biopsies derived from tonsillar carcinomas. we were able to demonstrate the presence of ebv-dna in the tumour cells of two poorly differentiated ... | 1983 | 6323895 |
| [analysis of viral capsid anti-antigen antibodies of the epstein barr virus in infectious mononucleosis. comparative evaluation of a fluorescence method with classic methods]. | 708 sera were tested by a fa method for the detection of antibodies anti-viral capsid antigen in epstein-barr virus disorders to establish its effective value in infectious mononucleosis diagnosis. the arise of igg class's antibody was not significative between recent infectious mononucleosis's patients and controls. preliminary study of a test to research igm anti-viral capsid antigen confirmed its value in infectious mononucleosis's diagnosis, but only in early stages of infection. at least, a ... | 1983 | 6330785 |
| the mlc response of patients with infectious mononucleosis to epstein-barr virus-transformed cells. | lymphocytes of 6 patients with infectious mononucleosis were found to be capable of responding in mixed lymphocyte culture (mlc) to epstein-barr virus (ebv)--transformed lymphoblastoid cell lines and raji cells, whereas their response to pwm-induced blasts and non-t cells was significantly depressed. this means that despite the suppression of cellular reactivity, observed in infectious mononucleosis, the specific response to ebv-infected cells remains unaffected. | 1983 | 6331340 |
| the epstein-barr virus (ebv)-transformed lymphoblastoid cell lines (lcl) as stimulating cells in mixed lymphocyte culture (mlc). | the mlc response of normal lymphocytes to different kinds of ebv-transformed lcl was investigated. the response to allogeneic lcl cells remarkably exceeded the response to xenogeneic and autologous lcl cells. stimulation performed with ebv-transformed and normal cells obtained from the same donors showed a correlation in the reaction levels (q = 0.434). this incomplete correlation was not improved by the reduction of the number of stimulating lcl cells. in mlc stimulated with lcl no statisticall ... | 1983 | 6331341 |
| studies of the epstein-barr virus receptor found on raji cells. i. extraction of receptor and preparation of anti-receptor antibody. | raji, a human lymphoblastoid cell line, expresses a membrane receptor (ebvr) specific for epstein barr virus (ebv). a component that binds ebv was extracted from this cell line by treatment of the cells for 3 hr on ice with tris buffer containing 10% glycerol. the treatment reduced the capacity of the cells to bind virus, and after concentration the receptor extract (re) inhibited both ebv binding and superinfection of fresh raji cells. similarly prepared extracts of ebvr- cells lacked such acti ... | 1983 | 6337215 |
| invasion of burkitt's lymphoma cell lines by yersinia enterocolitica. | we attempted to determine whether or not yersinia enterocolitica could invade two cell lines derived from burkitt's lymphoma (bl), p3hr-1, and raji cells, and if the expression of epstein-barr virus (ebv) genome could be activated by the invasion of the organisms into the cells. the invasiveness of y. enterocolitica into bl cells was examined morphologically, using transmission and scanning electron microscopy, and the induction of ebv antigens in the bl cells was examined by indirect immunofluo ... | 1983 | 6338506 |
| antibody moieties within circulating immune complexes in heart transplant recipients. | circulating immune complexes were isolated from the sera of cardiac allograft recipients by bovine conglutinin/anti-conglutinin co-precipitation, or by gel filtration and protein a-sepharose affinity chromatography. the antibody moieties within these isolated immune complexes were tested for specificity against heterologous anti-thymocyte globulins by solid phase radioimmunoassay, and bacterial and viral antigens by indirect immunofluorescence. the results showed that in addition to possessing s ... | 1983 | 6339123 |
| a human lymphocyte-associated antigen involved in cell-mediated lympholysis. | human lymphocyte function antigen (hlfa) is a cell surface protein defined by two monoclonal antibodies mhm23 and mhm24. it is present on both b and t lymphocytes but in greater amounts on the latter. both antibodies precipitated antigen, from radiolabeled hsb-2 cells, which ran as two chains on sodium dodecyl sulfate polyacrylamide gel electrophoresis at 180 and 94 kda. neither antibody inhibited binding of the other, indicating that distinct epitopes were recognized. both antibodies were shown ... | 1983 | 6339253 |
| development of a serum-free medium which supports the long-term growth of human and nonhuman primate lymphoid cells. | the ability to grow lymphoid cells in serum-free media affords the advantage of separately analyzing those components found to be involved in proliferation and differentiation. iscove's medium (imdm) supplemented with bovine serum albumin or casein, cholesterol, ferrous chloride, insulin, beta-mercaptoethanol, l-alpha-phosphatidylcholine, and transferrin supported the long-term proliferation of a gibbon ape lymphoma t-cell line, mla144. these cells continue to produce interleukin 2 (il-2, t-cell ... | 1983 | 6341383 |
| "in vitro" and "in vivo" leukocyte migration inhibitory factor production in acute infectious mononucleosis patients. | since infectious mononucleosis (im) mononuclear cells spontaneously release the leukocyte (migration) inhibitory factor (lif) in culture and since previous experiments in our laboratory have demonstrated that one or more substances with lif-like activity are detectable in the serum (serum lif) of young patients with heterophile-positive im, an investigation was carried out to determine both the in vitro and in vivo lif production in a series of im patients sampled during the acute phase of the i ... | 1983 | 6357231 |
| a comparative analysis of the phenotypic characteristics of available fusion partners for the construction of human hybridomas. | of several human fusion partners available for the production of monoclonal antibodies, only sko-007 and rpmi 8226 have phenotypic features characteristic for myeloma cells. cells from both lines exhibited abundant rough endoplasmic reticulum (rer) with a prominent golgi apparatus, few free ribosomes, condensed nuclear chromatin, and absence of the epstein-barr virus determined nuclear antigen (ebna). however, following prolonged passage of these lines, the amount of immunoglobulin (ig) producti ... | 1983 | 6332055 |
| two epstein-barr viral nuclear neoantigens distinguished by gene transfer, serology, and chromosome binding. | we recently identified, by means of cotransformation of ltk- cells, a region of the epstein-barr virus (ebv) genome (the bamhi k fragment) that encodes or induces an ebv nuclear neoantigen (ebna) serologically related to the ebna found in lymphoid cells carrying the entire ebv genome. we now find that a second ebv dna fragment, bamhi m, is also able to give rise to cotransformed ltk- cells with stable expression of a nuclear antigen. the bamhi k and m fragments have no apparent dna homology. man ... | 1983 | 6324183 |
| a sensitive assay to evaluate antistreptococcal antibodies using an in vitro-produced monoclonal human rheumatoid factor. | a human monoclonal rheumatoid factor (rf) produced in vitro by an epstein-barr virus (ebv) immortalized cell line is used in an assay to detect and quantitate antibodies directed against streptococcal antigens in human sera. the assay, based on the binding of the rheumatoid factor to igg-immune complexes, applies this monoclonal antibody in its iodinated form. the assay can easily quantitate igg-antistreptococcal antibodies and enables the quantitation of these specific antibodies in sera that c ... | 1983 | 6388967 |
| a lymphoblastoid cell line with high phagocytic activity established from peripheral blood of a patient with chronic myelocytic leukemia in blast crisis. | a human hematopoietic cell line (k-23-m) was established from a patient with chronic myelocytic leukemia in blast crisis. morphologically, the cultured cells were lymphoblastoid cells that produced iga and were epstein-barr viral nuclear antigen positive. but they showed high phagocytic activity to glutaraldehyde-treated sheep red cells and had properties of a monocyte or macrophage that included surface fc receptors, alpha-naphthyl butyrate esterase positivity blocked by naf, migration in soft ... | 1983 | 6321044 |
| the 53,000-dalton cellular protein and its role in transformation. | 1983 | 6321377 | |
| transformation of cultured cells by human herpesviruses. | 1983 | 6321380 | |
| establishment of a ph1 chromosome-positive cell line from chronic myelogenous leukemia in blast crisis. | a new hematopoietic cell line, designated kcl-22, was established in vitro by cultivation of pleural effusion cells obtained from a woman with chronic myelogenous leukemia in blast crisis. kcl-22 grew in suspension culture with a doubling time of 24 h and consisted of immature undifferentiated cells which were positive for periodic acid-schiff and acid phosphatase staining. chromosome analysis of the kcl-22 line showed a female karyotype with double ph1 chromosomes and additional chromosome abno ... | 1983 | 6321612 |
| circumstances favoring jaw tumors in burkitt's lymphoma. | in burkitt's lymphoma, dental structures may provide the route for epstein-barr virus (ebv) in saliva to penetrate the jaws, thereby promoting tumor formation. in children, ebv could enter tooth sockets exposed following deciduous tooth loss and thereby contact jaw marrow lymphocytes stimulating neoplastic transformation. marrow contact by ebv probably also occurs through carious teeth. jaw tumors are rare in adults because their jaw marrow is no longer hematopoietic and so lacks the lymphoid su ... | 1983 | 6321920 |
| a comparison of epstein-barr virus-specific t-cell immunity in rheumatoid arthritis and osteoarthritis patients. | the level of epstein-barr virus (ebv)-specific t-cell-mediated immunity in 20 rheumatoid arthritis (ra) patients was compared with 16 age- and sex-matched osteoarthritis (oa) patients using the regression of ebv-transformation assay. the results show that the level of ebv-specific t-cell immunity in ra patients is significantly depressed compared with oa patients (p less than .001) or healthy laboratory controls (p less than .001). in contrast, lymphocytes from ra and oa patients showed a simila ... | 1983 | 6318713 |
| pre-b cells in x-linked agammaglobulinemia. | 1983 | 6317082 | |
| [investigation of 2 families with clustering of nasopharyngeal carcinoma and detection of serum vca-iga antibody among their members]. | 1983 | 6317146 | |
| autoantibodies in three populations of burkitt's lymphoma patients. | autoantibodies against actin, intermediate filaments, single stranded dna and histones were found in the sera of burkitt's lymphoma (bl) patients and normal controls from tropical africa, where there is a high incidence of bl. both groups had significantly higher levels of these autoantibodies than caucasian normal controls or patients with other malignant diseases, as well as epstein-barr (eb) virus positive and eb virus negative bl patients from regions of north africa and france where the inc ... | 1983 | 6317239 |
| epstein-barr virus and infectious mononucleosis in children. | the presence of igg antibodies to epstein-barr virus (ebv) has been investigated in two groups of children aged 3 to 35 months. the test group consisted of 31 children from an institution in which a high incidence of suspected infectious mononucleosis (imn) was found. the control group comprised 54 children, aged 1 to 26 months, with noninfectious diseases. in the test group, 29 of the 31 children were positive for igg anti-ebv antibodies in the serum, whereas only 5 children were positive in th ... | 1983 | 6319323 |
| transfer of viral antibodies from mother to infant. | antibody titers to epstein-barr virus (ebv), cytomegalovirus (cmv), herpes simplex virus (hsv) type 1, varicella-zoster virus (vzv) and rubella virus (rv) were determined in maternal and in newborn cord sera in 258 consecutive deliveries. infants born with one or more of the following risk factors--weight less than 2.5 kg, apgar score less than 7, clinical jaundice, meconium-stained amniotic fluid, birth defects and respiratory distress syndrome--were considered to be at risk. similar geometric ... | 1983 | 6319326 |
| clinical and virological observations in childhood hodgkin's disease in israel. | antibody levels to epstein-barr virus (ebv), cytomegalovirus (cmv), herpes simplex virus (hsv) and varicella-zoster virus (vzv) were determined in children with hodgkin's disease (hd) before therapy, as well as in healthy, age-matched controls. included were 21 israeli-born children, aged 2.5 to 14.5 years: 13 jews whose parents were of african-asian origin, 1 jew whose parents were of european origin, and 7 arabs. most of them were from large families (average 5.5 children per family) and low s ... | 1983 | 6319327 |
| serologic diagnosis of nasopharyngeal carcinoma. a double-blind study of four eb virus antibodies with evaluation by sequential discrimination. | it is generally known that a close relationship exists between epstein-barr virus (ebv) and nasopharyngeal carcinoma (npc). recently, patients with early lesions of npc have been detected in the general population by use of serologic mass survey. using the double-blind method, we have studied the diagnostic value of the four ebv antibody titers, vca-iga, vca-igg, ea-iga and ea-igg, in four groups of subjects, each consisting of 50 persons: patients with nasopharyngeal carcinoma (npc group), pati ... | 1983 | 6319338 |
| aids: a medical conundrum. | 1983 | 6319462 | |
| [symptomatologic value of the assay of ebv antibodies in carcinoma of the nasopharynx]. | 1983 | 6319536 | |
| dna-binding proteins specified by herpesvirus saimiri. | herpesvirus saimiri-specific proteins from the nuclear fractions of productively infected owl monkey kidney cells were dissociated from virus and host dna by treatment with 2 m-nacl or separation on urografin density gradients. empty virus capsids remained intact and could be separated from major non-structural proteins (110k, 51k and 48k) and from a subset of structural proteins (130k, 29k and 12k), either by urografin gradient sedimentation or differential centrifugation. the dna in such solub ... | 1983 | 6319554 |
| [development of kawasaki disease and the initial infection by eb virus]. | 1983 | 6319775 | |
| [gianotti-crosti syndrome caused by epstein-barr virus]. | 1983 | 6318642 | |
| t-cell control of herpesvirus infections: lessons from the epstein-barr virus. | 1983 | 6320264 | |
| [study of virus-associated lymphomas in primates using a model of malignant lymphoma of the baboon]. | an outbreak of malignant lymphoma was registered in a population of hamadryas baboons. animals inoculated with blood from patients with various forms of leukemia were planted into the stock 10 years ago. 218 out of 3219 animals have already died from lymphoma. the morphological pattern of horizontally-spreading lymphoma was similar to basic cell patterns of hodgkin's and non-hodgkin lymphoma in man. as far as the immunologic classification of tumor lymphoid cells is concerned, baboon lymphomas w ... | 1983 | 6320540 |
| human-human b cell hybridomas from in vitro stimulated lymphocytes of patients with common variable immunodeficiency. | human-human b cell hybridomas have been established from the peripheral blood lymphocytes of patients with common variable immunodeficiency (cvi) by fusion with an hgprt-negative b lymphoblastoid cell line. igm-secreting hybridomas were successfully obtained from cvi lymphocytes after stimulation for 5 days in vitro with a combination of pwm and staphylococcus aureus strain cowan i. fusion of peripheral blood lymphocytes that were stimulated for 5 days in vitro with a single mitogen resulted in ... | 1983 | 6313803 |
| fatal illness in a 2-week-old infant: diagnosis by detection of epstein-barr virus genomes from a lymph node biopsy. | 1983 | 6313890 | |
| epstein-barr virus transcription in nasopharyngeal carcinoma. | sequences which encode epstein-barr virus (ebv) rna in nasopharyngeal carcinoma (npc) tissue have been identified. we utilized human biopsy material directly as well as npc grown in nude mice. total rna was extracted from the tumor material and separated into polyadenylated and nonpolyadenylated fractions by oligodeoxythymidylate-cellulose chromatography. this material was used as template to construct 32p-labeled cdna. the labeled cdnas were hybridized to southern blots of recombinant ebv dna f ... | 1983 | 6313960 |
| two distant clusters of partially homologous small repeats of epstein-barr virus are transcribed upon induction of an abortive or lytic cycle of the virus. | the two regions of the epstein-barr virus genome carrying partially homologous clusters of short tandem repeats (dsl and dsr [duplicated sequences, left and right, respectively] ) are transcribed into polyadenylated rna upon spontaneous or chemical induction of the lytic virus cycle. in raji, an epstein-barr virus genome carrying a nonproducer cell line, transcription of dsl and dsr is only observed upon induction of an abortive life cycle of the virus. in the nonproducer cell line raji, the pol ... | 1983 | 6313969 |
| [mechanism of human b lymphocyte activation. ii. determination of ebv-stimulated b cell function]. | 1983 | 6313993 | |
| recurrent cerebellitis. a case report of a possible relationship with epstein-barr infection. | 1983 | 6314037 | |
| [antibodies against non-structural antigens of epstein-barr virus in children with infectious mononucleosis]. | 1983 | 6314071 | |
| viruses and cancer: herpesviruses and tumors in the head and neck. a review. | 1983 | 6314221 | |
| chronic disseminated epstein-barr virus infection and humoral immunodeficiency: detection of viral antigen by elisa inhibition assay. | 1983 | 6314287 | |
| the association of epstein-barr virus with nasopharyngeal carcinoma. | 1983 | 6314350 | |
| vaccine control of malignancies induced by lymphotropic herpesviruses. | 1983 | 6314351 | |
| scanning immuno-electron microscopy of a monoclonal, epstein-barr virus(ebv) transformed human cell line producing rheumatoid factor in-vitro. | selected lymphocytes from a patient with rheumatoid arthritis were infected with epstein-barr virus (ebv) and the emerging cell line (rf-an) has now been successfully maintained in culture for more than two years. multiparameter studies, including surface markers, ultra-structure and scanning immuno-electron microscopy, were utilized to characterize and evaluate this unique human cell line. like other b-lymphoblastoid cell lines, rf-an cells display multiple microvilli, contain surface and intra ... | 1983 | 6314493 |
| [distribution of ebv-vca and ea antibody in the general population in a high incidence area of nasopharyngeal carcinoma]. | 1983 | 6317314 | |
| non-a, non-b hepatitis in hemodialysis. | episodes of presumable non-a, non-b hepatitis were determined among hemodialysis patients during a two year period as well as possible secondary infections in household contacts. we determined hepatitis a and b markers by commercial ria kits, and igm-anti-cmv and igm-anti-ebv by elisa techniques. 154 dialysis patients, 118 relatives of patients, and 42 members of the staff were included in the study. 71% of the center dialysis patients, 63% of the home dialysis patients, 50% of the staff, and 19 ... | 1983 | 6317495 |
| a 3q+ marker chromosome in ebv-carrying nasopharyngeal carcinomas. | the malignant epithelial cells of two anaplastic ebv-carrying nasopharyngeal carcinomas (npc) were investigated cytogenetically by giemsa banding technique. both tumors had a similar 3q+ marker chromosome with an involvement of band q25, either a duplication of the region q25-q27, or insertion of an unidentified segment at band q25. | 1983 | 6317575 |
| epstein-barr virus-associated antibody pattern in untreated non-hodgkin lymphoma patients. relationship to clinical variables and lymphocyte functions. | sera from 296 unselected and untreated patients with non-hodgkin lymphoma (nhl) classified according to the rappaport and the kiel systems were analyzed for antibodies to epstein-barr virus (ebv). the aim of the study was to determine whether antibody spectra and titers to ebv-coded antigens correlated to clinical and immunological variables and whether the titers were of any prognostic significance. increased antibody titers to eb viral capsid antigen (vca) and slightly raised titers to early a ... | 1983 | 6317577 |
| follow-up studies on epstein-barr virus iga/vca antibody-positive persons in zangwu county, china. | serological mass surveys were carried out in zangwu county, china, using an immunoenzymatic test. 3,533 persons were found to have epstein-barr virus (ebv) iga/vca antibody among 148,029 persons age 30 years and older who were tested during 1978-1980. among the iga/vca antibody-positive persons, 55 nasopharyngeal carcinoma (npc) cases were detected. follow-up studies were carried out yearly on the iga/vca antibody-positive persons for 1-3 years, and 32 additional npc patients were diagnosed. iga ... | 1983 | 6317603 |
| the epstein-barr virus-determined nuclear antigen: a previously identified 48k component and higher-molecular-weight forms of the antigen are structurally related. | the epstein-barr virus(ebv)-determined nuclear antigen (ebna) was purified from a variety of ebv-carrying nonproducer cells. antigen was detected by western blotting and subsequent visualization of complexes with antiserum/protein a. all cell lines contained several ebna components, ranging in molecular weight from 48,000 (48k) to 73k. even higher-molecular-weight forms of ebna-positive immunoreactivity were detected, but in much lower yield and in only some preparations (81k in raji; 120k in na ... | 1983 | 6317606 |
| an immunoprecipitation blocking assay for the analysis of ebv induced antigens. | a technique for the analysis of ebv antigens in extracts of unlabelled ebv infected cells has been developed. using this technique we have demonstrated that the ebv early antigen complex consists of several proteins and is not completely expressed in chemically induced raji cells. studies with a range of sera from patients with infectious mononucleosis and nasopharyngeal carcinoma have shown that despite similar titers by the indirect fluorescent antibody test different populations of proteins w ... | 1983 | 6317709 |
| use of cloned probes to detect epstein-barr viral dna in tissues of patients with neoplastic and lymphoproliferative diseases. | cloned fragments of the epstein-barr virus (ebv) genome were used to examine tissues from 145 patients for the presence of ebv dna by two techniques: (1) nucleic acid hybridization of cell spots from which the dna had been extracted in situ and (2) hybridization of dna that had been transferred to nitrocellulose by southern blotting. ebv dna was found in tissues from four adults and five children with american burkitt's lymphoma, infectious mononucleosis, lymphoma following bone marrow transplan ... | 1983 | 6317774 |
| burkitt lymphoma. ebv nucleoid-like structures and lack of capsids in lymphoblasts. | burkitt lymphoma biopsies and the csf malignancy have been investigated ultrastructurally. the tumor cells were found to contain vermicellar bodies formed by rod-like structures similar to those suggested to represent hsv nucleoids in hsv encephalities. but, unlike the hsv disease and bl in culture, no nucleocapsids nor empty capsids were detected. it is suggested that the rods in the burkitt lymphoblasts may well represent ebv nucleoids and that the absence of capsids may be another characteris ... | 1983 | 6317878 |
| [fluctuations of eb virus-related antibody titer associated with aging]. | 1983 | 6317916 | |
| lymphocyte subpopulations and reactivity during and after infectious mononucleosis. | t- and b-lymphocyte numbers, as well as lymphocyte reactivity to mitogens in vitro, were studied and correlated to other laboratory tests during the acute phase of infectious mononucleosis (30 patients) and up to one year thereafter. during the acute disease an absolute increase in both t- and b-lymphocyte numbers was recorded, the relative increase in b-lymphocytes occurring at the start. b-lymphocyte numbers fell after the second week and t-lymphocyte numbers after the fourth week of disease. ... | 1983 | 6317995 |
| [antibodies against human t-cell leukemia virus (htlv) in male homosexuals with acquired immunodeficiency syndrome]. | 1983 | 6318098 | |
| [burkitt's acute lymphoblastic leukemia. incidence and cytogenetic aspects]. | we report 4 cases of patients, 5, 16, 31 and 55 years old respectively, with burkitt type all. three of them presented with a hypoaesthesia of the chin tip. the cytological features of abnormal cells were those of l3 subgroup in the fab classification; they expressed surface immunoglobulin markers. ebv serology was positive in two cases but the titres did not indicate a recent infection. the translocation t(8;14) (q24;q32) was found in all 4 patients. in one case, it was possible to characterize ... | 1983 | 6318188 |
| [rheumatoid polyarthritis. the role of immune complexes]. | 1983 | 6318214 | |
| the science base underlying research on acquired immune deficiency syndrome. | in order to define the clinical syndrome of aids and begin to deal with it effectively, scientists needed to understand how the immune system works. fortunately, considerable knowledge was available: research in immunology over the last two decades had provided the technological advances and basic information about cell-mediated immunity that were necessary for identification of the syndrome. without this knowledge base, immune suppression would not have been recognized as the common link among ... | 1983 | 6318255 |
| prevalence of antibodies to epstein-barr virus (ebv) in childhood and adolescence in denmark. | a seroepidemiologic survey on the prevalence of antibodies to epstein-barr virus (ebv) in denmark was carried out. sera from 518 children and 178 adults were investigated for igg antibodies to the viral capsid antigen by an immunofluorescence assay. the proportion of subjects with antibodies increased from 15% at 1 yr to 60-65% in the 4-6 yr age group, was stationary at the age of 7-14, and then increased further progressively with age, to reach about 95% in late adulthood. among preschool child ... | 1983 | 6318303 |
| burkitt's lymphoma in thai children: an analysis of 25 cases. | twenty five cases of burkitt's lymphoma in thai children were diagnosed at the department of pediatrics, siriraj hospital during the period of 13 years (january 1969 to april 1982). males were more affected than females with the ratio of 1.7:1. the age ranged from 2 to 11 years with the median age of 4-5 years. the most common clinical manifestations were abdominal mass associated with nausea vomiting, abdominal pain, anorexia, weight loss and generalized lymphadenopathy which occurred in 50-60% ... | 1983 | 6318366 |
| failure to demonstrate concomitant antibody changes to viral antigens other than epstein-barr virus (ebv) during or after infectious mononucleosis. | a search for antibody rises to viral antigens other than to epstein-barr virus, the causative agent, has been carried out in serial serum samples from 82 patients with infectious mononucleosis (im). fourfold or greater rises in titer rarely occurred and did did not cluster in time. no rises occurred to cytomegalovirus, only 1.2 percent to herpes simplex virus, and 8.5 percent to varicella zoster virus. rises to measles antibody were found in 7.5 percent of patients and to rubella in 10.4 percent ... | 1983 | 6318461 |
| enzyme-linked immunosorbent assay for detection of antibodies to epstein-barr virus antigens. | enzyme-linked immunosorbent assay (elisa) was standardized for measurement of antibody activity of reference human and baboon (papio hamadryas) sera to soluble epstein-barr virus (ebv) antigens. a comparison with the immunofluorescent (if) method showed that elisa detects antibody specifically and sensitivity. in elisa, herpesvirus papio (hvp) nuclear antigen (hupna) positive baboon serum reacted with ebv nuclear antigen (ebna), as a further indication of the antigenic similarity between hvp and ... | 1983 | 6318497 |
| antibodies to herpes simplex and epstein-barr viruses in faroese children: association with sibship size, height and age. | antibody to herpes simplex virus (hsv) type 1 and epstein-barr virus (ebv) capsid antibody were determined in 333 children from the faroe islands, aged 4, 8 and 13 years. an analysis of multi-way frequency tables was performed, testing seropositivity for each virus against combinations of the following variables: sibship size, birth order, mother's age at birth, birth weight, actual height and weight, age and sex. hsv-seropositivity was associated to sibship size, height and age, while ebv-serop ... | 1983 | 6318512 |
| lifestyle and antiviral antibody studies among homosexual men in denmark. | two-hundred and fifty-nine male homosexuals (hs) of a large and a small danish town were studied for antibodies to various virus and their statistical relationship to sex habits, drug use and other lifestyle factors. prevalence rates against cytomegalovirus (cmv) were 86.7% of hs men from copenhagen and 73.3% of hs men from the smaller community of aarhus, against 30% of a control group. antibody prevalence in homosexuals was significantly related to number of years of homosexual activity (p les ... | 1983 | 6318513 |
| helper and suppressor t-cells regulating killer cells of eb virus infected cells. | using an in-vitro human lymphocyte system, a study was performed to determine whether helper t-cells and suppressor t-cells control the generation of killer cells against autologous b-cells transformed by epstein-barr (eb) virus. when t-cells were treated with mitomycin c and reacted in the presence of macrophages with target cells bearing hla-dr, helper t-cells were induced but suppressor t-cells were not. in contrast, when t-cells were reacted in the absence of macrophages to target cells lack ... | 1983 | 6314594 |
| an epstein-barr virus-negative b lymphoma cell line (thp-2) from a burkitt's lymphoma of a japanese patient. | an epstein-barr virus-negative lymphoma cell line, thp-2, was established from a burkitt's lymphoma taken from a japanese patient. thp-2 cells grew in single cell suspension with a doubling time of 24 hr; the cells carried surface-bound mu-lambda immunoglobulins and formed rosettes with igg antibody-coated ox erythrocytes. thp-2 cells expressed b1, common acute lymphoblastic leukemia (all) (j5) and ia-like antigens of their surface as defined by monoclonal antibodies. epstein-barr virus-associat ... | 1983 | 6314596 |
| epstein-barr virus antibody titers in cancer of the head and neck. | pretreatment serum samples from 39 white american patients with epidermoid cancer of various sites of the head and neck, including the nasopharynx, were studied for both iga and igg antibodies to the epstein-barr virus (ebv)-specific antigens. of the various serological assays, igg anti-viral capsid antigen (vca) was least specific and was present frequently at high titers in both the groups with nasopharyngeal carcinoma (npc) and groups with other cancers (oc). the geometric mean titer (gmt) of ... | 1983 | 6314955 |
| [clinical aspects of virus-induced tumors]. | 1983 | 6314957 | |
| inhibition of 12-o-tetradecanoylphorbol-13-acetate-induced ornithine decarboxylase activity in mouse epidermis by sweetening agents and related compounds. | the effects of naturally occurring sweetening agents, which inhibited the induction of epstein-barr virus-associated early antigen (ebv-ea) induced by 12-o-tetradecanoylphorbol-13-acetate (tpa), and related compounds on the induction of ornithine decarboxylase (odc) by tpa is examined. application of glycyrrhetinic acid or steviol to mouse skin 1 h before tpa treatment showed a remarkable decrease in tpa-induced odc activity. post-treatment with glycyrrhetinic acid or steviol 1 h after applicati ... | 1983 | 6315213 |
| immunological properties of lymphoblastoid cell lines (uhkt) - 1, 2, 3, 4, 5, 6) established from cases of human leukemias. | immunological characteristics of stabilized cell lines (uhkt-1, 2, 3, 4, 5, 6) established from cells of peripheral blood of patients with leukemia were studied. attention was focussed on the analysis of various membrane markers (e. g. t, ia-like, antigens, membrane immunoglobulin, receptors for sheep erythrocytes, for igg-fc fragment, for the third component of the complement and expression of beta-2 microglobulin). the cells of these lines were also tested for the presence of epstein-barr viru ... | 1983 | 6315324 |
| acyclovir. a review of its pharmacodynamic properties and therapeutic efficacy. | acyclovir (aciclovir) is a nucleoside analogue antiviral drug related to cytarabine, idoxuridine, trifluridine and vidarabine. in common with these earlier antivirals, acyclovir is active against some members of the herpesvirus group of dna viruses. the efficacy of topical acyclovir has been convincingly demonstrated in ocular herpetic keratitis, and in initial and primary initial genital herpes infection, but little or no clinical benefit was seen when non-primary initial genital infections wer ... | 1983 | 6315332 |
| common multigenic activation in different human neoplasias. | it was previously shown that a common multigenic component, designated as tumor-specific dna (tsdna), was transcriptionally active in human lymphoid neoplasias and only slightly active, if at all, in normal lymphoid cells, including the priess cell line immortalized by epstein-barr virus. in the burkitt lymphoma-derived raji cell line, tsdna corresponded to 2500-3000 distinct transcription units, arbitrarily defined as encoding mrna chains of 1000 kds each. in the present study, radioactive raji ... | 1983 | 6315395 |
| transfer of the epstein-barr virus genes coding for small rnas to human lymphoid cells with a vector carrying a dominant selectable marker. | epstein-barr virus (ebv)-negative, burkitt-like lymphoma-derived cells were transformed with a transducing vector (psv2-gpt) containing the escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase (xgprt) and with a derivative of psv2-gpt that carries the genes for the ebv-associated small rnas on the ecori j fragment of b95-8 ebv dna inserted at the unique ecori site (pj-gpt). cells transformed with psv2-gpt and pj-gpt express the e. coli gpt gene to approximately the same ex ... | 1983 | 6315418 |
| epstein-barr virus markers in a series of burkitt's lymphomas from the west nile district, uganda. | in an epidemiological survey in the west nile district of uganda, 70 pathologically confirmed bl cases were detected over a 5-yr period; this corresponded to an annual incidence rate of 1.6 per 100,000 general population or about 5 per 100,000 children in the age group 5-14 yr. of the confirmed cases which were examined by ebv/dna molecular hybridization, 96% were found to contain an average of 38 ebv genome equivalents per tumour cell, whereas none of the examined unconfirmed cases did. duplica ... | 1983 | 6315443 |
| direct action of cyclosporin a on human b-lymphocytes with regard to immunoglobulin production. | highly purified preparations of human b-lymphocytes were cultured with or without cyclosporin a (cya; 1 microgram/ml) for 8 days with pokeweed mitogen (pwm) in the presence of helper factors or with epstein-barr virus (ebv). the amounts of igg, igm, iga and ige produced in cultures were measured by radioimmunoassays or by reverse hemolytic plaque-forming cell assays. the results demonstrate that whereas cya had a strong suppressive effect on the production of immunoglobulins (ig) by pwm-activate ... | 1983 | 6315572 |
| presence of ebv-dna sequences in nasopharyngeal cells of individuals without iga-vca antibodies. | exfoliated nasopharyngeal (np) cells from 62 normal cantonese chinese having iga/vca antibodies for more than a year and from 39 similar persons without iga/vca antibodies, were tested for the presence of ebv/dna sequences by spot followed by blot hybridization tests, using the cloned internal repeat of b95-8 viral dna as probe. thirteen out of 62 specimens from iga/vca-positive (21%) and six out of 39 specimens (15.4%) from iga/vca-negative individuals were found to contain ebv/dna sequences. f ... | 1983 | 6315600 |
| japanese burkitt's lymphoma: clinicopathological review of 14 cases. | the clinicopathological features of 14 japanese patients with burkitt's lymphoma (seven studied by us and seven reported by others) are reviewed. five patients were 12 years of age or younger. six patients presented with abdominal masses and five with jaw tumors. twelve patients manifested blood and/or marrow involvement at the time of initial diagnosis (four patients) or later in the course of the disease (eight patients). most of the patients had a rapid clinical course in spite of combination ... | 1983 | 6315992 |