Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| herpes simplex virus type 2 growth and latency reactivation by cocultivation are inhibited with antisense oligonucleotides complementary to the translation initiation site of the large subunit of ribonucleotide reductase (rr1). | antisense oligonucleotides complementary to the translation initiation site of the herpes simplex virus type 2 (hsv-2) large subunit of ribonucleotide reductase (rr1) were studied for their ability to inhibit rr1 expression, hsv-2 growth, and its reactivation from latently infected ganglia. the oligomers caused a significant decrease (90%-97% inhibition) in hsv-2 rr1 expression and inhibited hsv-2 growth, with ic50 and ic90 values of 0.11 and 1.0 microm, respectively. the titers of hsv-2 mutants ... | 2000 | 10805158 |
| [the role of different microorganisms in the genesis of tubal-peritoneal infertility and in immunity]. | the character of microflora, directly causing inflammatory process in tuboperitoneal infertility, was studied. the study revealed the prevalence of microbial associations, chlamydia infection was detected in half of the women, and in 17% of the patients this infection was for the first time detected in the upper section of their reproductive apparatus, which was indicative of the role of chlamydia infection in the occlusion of the uterine tubes. opportunistic microflora persisting in persons wit ... | 2000 | 10808586 |
| decreased vaginal disease in j-chain-deficient mice following herpes simplex type 2 genital infection. | j-chain-deficient (jch(-/-)) mice were used to study the role of polymeric iga (piga) in primary disease and protective immunity following genital herpes simplex type 2 (hsv-2) infection. vaginal iga in the jch(-/-) mice was composed primarily of monomeric iga and was not associated with secretory component (sc). in contrast, vaginal iga in wild-type (wt) mice was predominantly polymeric and bound to sc. following hsv-2 genital infection, the jch(-/-) mice consistently exhibited fewer vaginal sy ... | 2000 | 10814580 |
| virological diagnosis of herpes simplex encephalitis. | the herpes simplex encephalitis (hse) represents one of the most severe infectious diseases of the central nervous system. as effective antiviral drugs are available, rapid and reliable diagnosis has become important. | 2000 | 10814936 |
| hsv-2 specific serology should be offered routinely to antenatal patients. | the most devastating consequence of genital herpes is neonatal herpes. it is clear that the majority of newborns acquire their infection by contact with infected genital secretions during delivery from an asymptomatic mother who acquired a first episode of genital herpes near the time of labour. since the majority of cases of first episode genital herpes during pregnancy are unrecognised, the prevention of neonatal transmission will depend upon the identification of the hsv serologically discord ... | 2000 | 10815025 |
| hsv-2 specific serology should not be offered routinely to antenatal patients. | the proposal to introduce antenatal screening for hsv has no evidence for a public or individual health benefit; indeed, it has the potential to increase anxiety for patients, with a minimal likelihood of reducing the risk of neonatal herpes infection. antenatal screening of an essentially healthy population of women must be validated in settings of different rates of neonatal hsv infection and the purposes and limitations of screening clearly outlined. identification of pregnant women at risk o ... | 2000 | 10815026 |
| herpes simplex virus peritonitis. | 2000 | 10816164 | |
| difference in incidence of spontaneous mutations between herpes simplex virus types 1 and 2. | spontaneous mutations within the herpes simplex virus (hsv) genome are introduced by errors during dna replication. indicative of the inherent mutation rate of hsv dna replication, heterogeneous hsv populations containing both acyclovir (acv)-resistant and acv-sensitive viruses occur naturally in both clinical isolates and laboratory stocks. wild-type, laboratory-adapted hsv type 1 (hsv-1) strains kos and cl101 reportedly accumulate spontaneous acv-resistant mutations at a frequency of approxima ... | 2000 | 10817703 |
| premarket evaluation of the pockit hsv-2 type-specific serologic test in culture-documented cases of genital herpes simplex virus type 2 [see comment]. | the genital herpes epidemic continues, in part, because patients with subclinical or atypical presentations cannot be identified by most herpes simplex virus (hsv) antibody tests. a new product, pockit hsv-2, has been developed to rapidly and accurately detect antibodies to hsv type 2 (hsv-2) in capillary blood or serum. | 2000 | 10821598 |
| testing for herpes simplex virus type 2: full-steam ahead? | 2000 | 10821599 | |
| seroprevalence of herpes simplex virus type 2 infection among attendees of a sexually transmitted disease clinic in italy. italian herpes forum. | an increased prevalence of herpes simplex virus type 2 (hsv-2) infection has been recently observed in industrialized countries. | 2000 | 10821604 |
| synthesis and antiviral activity of acyclovir-5'-(phenyl methoxy alaninyl) phosphate as a possible membrane-soluble nucleotide prodrug. | we describe a synthesis of acyclovir-5'-(phenyl methoxy alaninyl) phosphate (2) from acyclovir (1). this compound was designed to act as a lipophilic, membrane-soluble prodrug of the free nucleotide. however, the biological activities of this derivative against a range of viruses indicated poor intracellular phosphate delivery, in marked contrast to the earlier successful delivery of several dideoxy anti-hiv nucleotides. | 2000 | 10762044 |
| use of herpes simplex virus (hsv) type 1 iscoms 703 vaccine for prophylactic and therapeutic treatment of primary and recurrent hsv-2 infection in guinea pigs. | the effect of subunit vaccination on the incidence and severity of primary and recurrent genital herpes was investigated in the female guinea pig model of herpes simplex virus (hsv) type 2 genital infection. after prophylactic immunization with zwitterionic detergent-solubilized hsv-1 glycoproteins formulated with alhydrogel or as immunostimulating complex particles, significant reductions in the incidence and severity of primary herpetic illness were observed in both vaccinated groups compared ... | 2000 | 10762560 |
| herpes simplex virus type 1 as a cause of genital herpes: impact on surveillance and prevention. | this study compared characteristics of patients who had herpes simplex virus (hsv) type 1 with characteristics of patients who had hsv-2, by use of data from a cross-sectional analysis. data were collected in an urban sexually transmitted diseases clinic from patients who had positive genital hsv cultures. overall, 17.1% (95% confidence interval [ci], 14.9%-19.3%) of 1145 genital hsv isolates obtained during 1993-1997 were hsv-1. the proportion of hsv-1 among initial genital herpes infections wa ... | 2000 | 10762576 |
| characterization of an acyclovir-resistant herpes simplex virus type 2 strain isolated from a premature neonate. | acyclovir resistance is not a recognized problem among neonates with perinatal herpes simplex virus (hsv) infection. a premature newborn with neurocutaneous hsv infection was treated for 21 days with acyclovir. disseminated disease recurred 8 days later. a recurrent isolate was resistant to acyclovir and lacked thymidine kinase activity on the basis of a frameshift mutation in the thymidine kinase (tk) gene. compared with the sensitive isolate obtained during primary infection, replication of th ... | 2000 | 10762577 |
| impact of antivirals and emergence of drug resistance: hsv-2 epidemic control. | genital herpes, caused by herpes simplex virus type-2 (hsv-2), affects more people world-wide than any other sexually transmitted disease (std). antivirals are effective in decreasing the duration of symptoms and in reducing viral shedding; however, currently antiviral usage is extremely low. increased usage of antivirals would have a beneficial epidemic-level effect (due to the decreased transmission of drug-sensitive strains) as well as potentially a detrimental epidemic-level effect (if drug- ... | 2000 | 10763542 |
| susceptibility of clinical strains of herpes simplex virus to three nucleoside analogues. | the susceptibility of clinical isolates of herpes simplex virus 1 (hsv1) (58 strains) and 2 (hsv2) (17 strains) from the centre hospitalier et universitaire de nancy to three nucleoside analogues was compared by the dye uptake method. as expected, all strains of hsv2 were resistant to brovavir or sorivudine. aciclovir and penciclovir activities were comparable; 2 strains of hsv1 were resistant to these two compounds. four strains isolated from immunocompromised patients gave different results wi ... | 2000 | 10765035 |
| antiviral activity of ganciclovir elaidic acid ester against herpesviruses. | a fatty acid derivative of ganciclovir (gcv), elaidic acid ganciclovir (e-gcv), has been evaluated for its inhibitory activity against laboratory and clinical strains of herpes simplex type 1 (hsv-1) and type 2 (hsv-2), varicella-zoster virus (vzv) and human cytomegalovirus (hcmv) in human embryonic lung fibroblasts. gcv, cidofovir, acyclovir (acv), brivudin (bvdu) and foscarnet (pfa) were included as reference compounds. the viruses studied were wild-type, thymidine kinase-deficient (tk(-)) and ... | 2000 | 10771080 |
| synthesis and antiviral activities of enantiomeric 1-[2-(hydroxymethyl) cyclopropyl] methyl nucleosides. | cyclopropyl carbocyclic nucleosides have been synthesized from the key intermediate 2 which was converted to the mesylated cyclopropyl methyl alcohol 3. condensation of compound 3 with various purine and pyrimidine bases gave the desired nucleosides. all synthesized nucleosides were evaluated for antiviral activity and cellular toxicity. among them adenine 22 and guanine 23 derivatives showed moderate antiviral activity against hiv-1 and hbv. none of the other compounds showed any significant an ... | 2000 | 10772713 |
| efficacy of topical acyclovir monophosphate, acyclovir, or penciclovir in orofacial hsv-1 infections of mice and genital hsv-2 infections of guinea pigs. | the purpose of these studies was to compare the efficacy of acyclovir monophosphate (acvmp), acyclovir (acv), or penciclovir (pcv) against hsv-1 in an orofacial infection of mice and against acv sensitive and resistant genital hsv-2 infections of guinea pigs. treatment was initiated 24, 48, or 72 hours post inoculation with 5% acvmp, 5% acv (zovirax) or 1% pcv (denavir). in all experiments, similar efficacy was obtained for acvmp and acv, whereas pcv was considerably less effective. | 2000 | 10772730 |
| [the functions of alpha herpesvirus gene products]. | herpes simplex virus type 1(hsv-1), type 2(hsv-2) and varicella-zoster virus (vzv) belong to alphaherpesvirus family. these herpesviruses have large dna genomes which contain at least 69 genes. these viral genes are divided into two groups based on whether they are essential or dispensable for virus growth in cell culture. the essential gene products include transcriptional factor, viral dna replication, dna cleavage/packaging and virion components, while the dispensable gene products include de ... | 2000 | 10774196 |
| [seroepidemiology of alphaherpesviruses]. | the seroepidemiology of herpes simplex virus (hsv) type-1 and -2 was studied in different japanese populations, by applying hsv gg1 and gg2 type-specific antibody assays. hsv-1 infections correlated mostly with age and was widely prevalent among subjects over 40 years old. hsv-2 prevalence varied greatly among subgroups defined by sexual activity and were associated with risk behaviors, from 80% among prostitutes to 7% among pregnant women. since hsv-1 infection during childhood has been decreas ... | 2000 | 10774202 |
| [alpha-herpesvirus infection in the central nervous system]. | herpes simplex virus type 1 (hsv1) encephalitis is commonly observed, but hsv1 myelitis is rare. on the other hand, hsv2 is common etiologic agent for ascending necrotizing myelitis associated with diabetes mellitus, malignant tumors, aids and immunocompromised hosts. it is suggested that latent infection of the virus of root ganglia or primary infected virus is possibly followed by spread of infection to the central nervous system, particularly in immunocompromised patients. in this report, pat ... | 2000 | 10774207 |
| [sexually transmitted diseases of alpha herpes virus in women]. | among two alpha herpes viruses, herpes simplex virus(hsv) and varicella zoster virus(vzv), hsv infects genital sites and is frequently transmitted by sexual contact while vzv has quite different mode of transmission and rarely infects genital site except herpes zoster at the vulva. genital herpes is the second and the third leading cause of stds in women and men respectively. while 90% of male genital herpes was caused by hsv-2, 55% of female genital herpes was by hsv-2 and the remaining 45% by ... | 2000 | 10774210 |
| [ophthalmic area]. | although herpes simplex virus type 1 or herpes zoster virus is generally considered to cause ocular infection among alpha herpesviruses, herpes simplex virus type 2(hsv-2) retinitis has been recently reported. acute retinal necrosis(arn) syndrome is associated with herpesviruses, but the mechanisms remain unclear. the representative ocular infection by alpha herpesviruses is herpes simplex virus type 1(hsv-1) keratitis. the clinical types, ocular symptoms and therapy are reviewed in herpetic ocu ... | 2000 | 10774213 |
| risk factors for pre-cancerous lesions of the cervix. | studies on risk factors for pre-cancerous lesions of the uterine cervix have shown strong association with sexual practice. women with multiple sexual partners and intercourse at early age are at high risk. a role of male partners in further enhancing the risk has been identified. all these support the hypothesis relating to a sexually transmissible aetiological agent. an extensive review of the literature on the risk factors for pre-cancerous lesions of cervix has been carried out. the risk fac ... | 2000 | 10777005 |
| polyherbal formulations with wide spectrum antimicrobial activity against reproductive tract infections and sexually transmitted pathogens. | recent reports indicate high incidence of genital infections, most of which are sexually transmitted. although specific drugs and antibiotics are available for some, a safe spermicidal formulation with wide spectrum antimicrobial action would be a desirable addition to the presently available spermicides. | 2000 | 10735590 |
| hydrogels containing monocaprin prevent intravaginal and intracutaneous infections with hsv-2 in mice: impact on the search for vaginal microbicides. | hydrogel formulations containing the 1-monoglyceride of capric acid (monocaprin) possess potent in vitro microbicidal activity against hiv and hsv, chlamydia trachomatis and neisseria gonorrhoeae. these formulations were studied to determine whether they prevent intracutaneous and intravaginal infections of mice with hsv-2, a virus that is in vitro as sensitive to the virucidal action of the compound as is hiv. in mice intravaginal infection with hsv-2 and the associated mortality was prevented ... | 2000 | 10745241 |
| neonatal herpes simplex virus type ii. | this description of a tragic case of infection with neonatal herpes simplex virus type ii is presented to alert professional nurses to the fact that this disease is sometimes difficult to recognize, but must be considered when a newborn has any unusual symptoms. neonatal herpes simplex type ii may have a slow and insidious onset, and be masked by many other symptoms. even though the virus might be present in a newborn, the symptoms might not be apparent until several days after birth; this is a ... | 2000 | 10748589 |
| preclinical evaluation of "whole" cell vaccines for prophylaxis and therapy using a disabled infectious single cycle-herpes simplex virus vector to transduce cytokine genes. | the development of genetically modified "whole" tumor cell vaccines for cancer therapy relies on the efficient transduction and expression of genes by vectors. in the present study, we have used a disabled infectious single cycle-herpes simplex virus 2 (disc-hsv-2) vector constructed to express cytokine or marker genes upon infection. disc-hsv-2 is able to infect a wide range of tumor cells and efficiently express the beta-galactosidase reporter gene, granulocyte-macrophage colony-stimulating fa ... | 2000 | 10749137 |
| herpes simplex virus type-1 and -2 pathogenesis is restricted by the epidermal basement membrane. | murine flank scarification with hsv-1 and -2 results in primary lesions at the site of inoculation within three days and lesions at secondary sites within four days. the severity of the infection can be given a numerical value or "score" which is derived from the number and size of these lesions. using this model, we investigated the role of the epidermal basement membrane in hsv pathogenesis. we exposed murine epidermis to 5 x 10(4) plaque forming units of hsv-1 and -2, which by day 8 produced ... | 2000 | 10752560 |
| herpes simplex virus ul17 protein is associated with b capsids and colocalizes with icp35 and vp5 in infected cells. | a previous study using a mutant lacking the ul17 gene has suggested that the ul17 protein of herpes simplex virus type 1 (hsv-1) is required for the cleavage/packaging of viral dna. in this study, we have raised a rabbit polyclonal antiserum which specifically reacted with the ul17 protein which has an apparent molecular mass of 78-kda in the lysates of hsv types 1- and 2-infected vero cells. western blot analysis of intracellular capsids demonstrates that the ul17 protein was associated with b ... | 2000 | 10752563 |
| hla-dq tetramers identify epitope-specific t cells in peripheral blood of herpes simplex virus type 2-infected individuals: direct detection of immunodominant antigen-responsive cells. | ag-specific cd4+ t cells are present in peripheral blood in low frequency, where they undergo recruitment and expansion during immune responses and in the pathogenesis of numerous autoimmune diseases. mhc tetramers, which constitute a labeled mhc-peptide ligand suitable for binding to the ag-specific receptor on t cells, provide a novel approach for the detection and characterization of such rare cells. in this study, we utilized this technology to identify hla dq-restricted ag-specific t cells ... | 2000 | 10754321 |
| nectin2alpha (prr2alpha or hveb) and nectin2delta are low-efficiency mediators for entry of herpes simplex virus mutants carrying the leu25pro substitution in glycoprotein d. | the receptors for entry of herpes simplex viruses 1 and 2 (hsv-1 and -2), widely expressed in human cell lines, are members of a subset of the immunoglobulin superfamily exemplified by herpesvirus entry mediator c (hvec) and the herpesvirus immunoglobulin-like receptor (higr). this report focuses on two members of this subset, herpesvirus entry mediator b (hveb), recently designated nectin2/prr2alpha, and its splice variant isoform, nectin2/prr2delta. nectin2alpha and -delta share the ectodomain ... | 2000 | 10627537 |
| analysis of candidate-host immunogenetic determinants in herpes simplex virus-associated mollaret's meningitis. | infection due to herpes simplex virus (hsv) is associated with recurrent aseptic meningitis (mollaret's meningitis); however, the neuropathogenesis of this disease remains unknown. we collected 20 cerebrospinal fluid (csf) specimens that were positive for hsv dna by using polymerase chain reaction (pcr) assay from patients with a clinical diagnosis of mollaret's meningitis. patients were predominantly female (female:male, 22:1), with an average age of 32.8 years (range, 18-46 years). using direc ... | 2000 | 10619748 |
| cervical shedding of herpes simplex virus in human immunodeficiency virus-infected women: effects of hormonal contraception, pregnancy, and vitamin a deficiency. | genital shedding of herpes simplex virus (hsv) results in frequent transmission of infection to sexual partners and neonates. in a cross-sectional study, cervical shedding of hsv dna was detected in 43 (17%) cervical swab samples from 273 women seropositive for hsv-1, hsv-2, and human immunodeficiency virus type 1 (hiv-1). cervical shedding of hsv was significantly associated with oral contraception (adjusted odds ratio [aor], 4.5; 95% confidence interval [ci], 1.7-12.2), use of depo-medroxyprog ... | 2000 | 10608751 |
| limited antibody-dependent cellular cytotoxicity antibody response induced by a herpes simplex virus type 2 subunit vaccine. | the humoral response to a herpes simplex virus (hsv) type 2 subunit vaccine containing recombinant glycoproteins b (gb2) and d (gd2) was tested in 3 groups of patients. these included hsv-seronegative, hsv-1-seropositive, and hsv-2-seropositive individuals. there were excellent antibody responses, as measured by gb2- and gd2-specific elisas and hsv-2 neutralization assays. however, in 2 hsv-2 antibody-dependent cellular cytotoxicity (adcc) assays, there were relatively low antibody responses, es ... | 2000 | 10608784 |
| etiology of sexually transmitted infections among street-based female sex workers in dhaka, bangladesh. | an etiological study of sexually transmitted infections (stis) was conducted among female sex workers (fsws) in dhaka, bangladesh. endocervical swab and blood samples from 269 street-based fsws were examined for neisseria gonorrhoeae, chlamydia trachomatis, and trichomonas vaginalis as well as for antibodies to treponema pallidum and herpes simplex virus 2 (hsv-2). sociodemographic data and data regarding behavior were also collected. a total of 226 of the 269 fsws (84%) were positive for the st ... | 2000 | 10699032 |
| therapeutic vaccination against hsv-2: influence of vaccine formulation on immune responses and protection in mice. | therapeutic immunisation may represent a means of influencing viral infections that persist in the host by modulating the nature or level of host immunity. to assess the influence of the form of the antigenic stimulus on immunity to type-2 herpes simplex virus (hsv-2), mice pre-infected with sublethal doses of hsv-2 were immunised with various hsv-2 vaccine formulations prior to challenge infection with heterologous hsv-1. measurements of interleukin-2 (il-2), interleukin-4 (il-4) and interferon ... | 2000 | 10699326 |
| dna immunization utilizing a herpes simplex virus type 2 myogenic dna vaccine protects mice from mortality and prevents genital herpes. | a gene transfer vector for dna immunization was developed in which the promoter was derived from the murine muscle creatine kinase (mck) gene; a gene expressed only in differentiated skeletal muscle. in vitro, we observed high-level, but unrestricted, gene expression from the cytomegalovirus (cmv) promoter unlike expression from the mck promoter which was weak but restricted to myofibers. a myogenic dna vaccine (mdv) that encoded the glycoprotein d gene from herpes simplex virus type-2 (hsv-2) w ... | 2000 | 10699332 |
| [herpes simplex virus type 1 and 2 in switzerland]. | a worldwide increase in the incidence of genital herpes infections has been described in recent years. transmission of the herpes simplex virus type 2 (hsv-2) by asymptomatic seropositive subjects is considered to be a relevant mode of infection. the seroprevalence of hsv-2 varies considerably between different populations. in europe, data are scarce and the epidemiological situation in switzerland is unknown. in 1997 we performed serological examinations in 151 adult volunteers (87% between 20 ... | 2000 | 10701231 |
| high prevalence of herpes simplex virus type 2 in acute retinal necrosis syndrome associated with herpes simplex virus in japan. | to determine the type of herpes simplex virus in acute retinal necrosis syndrome associated with herpes simplex virus. | 2000 | 10704570 |
| enhancement of antiherpetic activity of cytarabine by hydroxyurea in human embryonic skin-muscle fibroblasts. | pharmacological induction of low deoxyribonucleoside triphosphate (dntp) levels is a novel approach to combine inhibition of virus replication. in accordance with this concept, the alteration of antiherpes activity of cytarabine (arac) in combination with hydroxyurea (hu), an inhibitor of ribonucleotide reductase, was studied. the results were confirmed by virus yield assays in human embryonic skin-muscle fibroblasts (hesmf). it was demonstrated that hu significantly enhanced the antiviral activ ... | 2000 | 10705616 |
| necrotizing ulcerative stomatitis in human immunodeficiency virus-seropositive individuals: a review of the histopathologic, immunohistochemical, and virologic characteristics of 18 cases. | the purpose of this retrospective study was to delineate the histopathologic, immunohistochemical, and virologic characteristics of 18 cases of necrotizing ulcerative stomatitis. | 2000 | 10710458 |
| antibody responses, cytokine levels and protection of mice immunised with hsv-2 antigens formulated into nisv or iscom delivery systems. | the immunogenicity of a type 2 herpes simplex virus (hsv-2) antigen preparation following its formulation into immunostimulating complexes (iscoms) or non-ionic surfactant vesicles (nisv) was investigated in a murine model. the immune responses induced by each formulation were characterised by antigen specific total and subclass serum responses, and by lymphocyte proliferation and cytokine (interleukin-2 (il-2), interleukin-4 (il-4) and interferon-gamma (ifn-gamma)) production by in vitro restim ... | 2000 | 10715522 |
| anti-herpes simplex virus activities of crude water extracts of thai medicinal plants. | a number of thai medicinal plants, recommended as remedies for herpesvirus infection and have been used in primary health care were investigated for their intracellular activities against herpes simplex viruses (hsv). centella asiatica l., maclura cochinchinensis cornor, and mangifera indica l. contained both anti-hsv-1 and -2 activities, as determined by plaque inhibition assay. an inhibition of the production of infectious hsv-2 virions from infected vero cells could also be demonstrated. comb ... | 2000 | 10715843 |
| anti-herpes simplex virus component isolated from maclura cochinchinensis. | the powerful anti-herpes simplex virus (hsv) activity of maclura cochinchinensis in vitro prompted us to carry out biologically-guided separation of the active component(s). ethyl acetate and methanol extracts exhibited anti-hsv-2 activity at ec50 values of 38.5 micrograms/ml and 50.8 micrograms/ml, respectively. although petroleum ether extract was inactive, a chloroform extract was too toxic to the test cell culture to perform the test. biologically-guided chromatographic separation of the eth ... | 2000 | 10715844 |
| the cause of invasive cervical cancer could be multifactorial. | cancer of the cervix is the third most common cancer among women worldwide, with incidence rates ranging from 3.8 per 100,000 women per year in israel to 48.2 per 100,000 per year in colombia. epidemiologic and clinical data suggest that human papillomaviruses, especially hpv-16 and hpv-18, play the major role in the etiology of cervical cancer. however, many investigators acknowledge that hpv is neither necessary nor sufficient in the etiology of cervical cancer and that a multifactorial etiolo ... | 2000 | 10721464 |
| antiviral properties of a series of 1,6-naphthyridine and 7, 8-dihydroisoquinoline derivatives exhibiting potent activity against human cytomegalovirus. | a series of 1,6-naphthyridine (l. chan, h. jin, t. stefanac, j. f. lavallee, g. falardeau, w. wang, j. bedard, s. may, and l. yuen, j. med. chem. 42:3023-3025, 1999) and isoquinoline (l. chan, h. jin, t. stefanac, w. wang, j. f. lavallee, j. bedard, and s. may, bioorg. med. chem. lett. 9:2583-2586, 1999) analogues exhibiting a high level of anti-human cytomegalovirus (hcmv) activity were investigated in a series of studies aimed at better understanding the mechanism of action of some representat ... | 2000 | 10722493 |
| immunisation with phage displaying peptides representing single epitopes of the glycoprotein g can give rise to partial protective immunity to hsv-2. | filamentous phage displaying peptides representing single epitopes of the glycoprotein g of hsv-2 (gg2) were used as immunogens via the subcutaneous route in balb/c mice without additional adjuvant. the phage were isolated from a random phage peptide display library and contain 15-mer peptide inserts that mimic epitopes of gg2. in each case, an antibody response to gg2 was generated that was dependent on the dose of phage administered and on the presence of the peptide insert. phage displaying e ... | 2000 | 10725197 |
| glycoprotein g of herpes simplex virus type 1: identification of type-specific epitopes by human antibodies. | serological diagnosis of herpes simplex virus (hsv) infections requires assays based on antigens that expose type-specific determinants. this study was designed to outline the b-cell epitopes of the type-specific glycoprotein g-1 (gg-1) of hsv type 1 (hsv-1), by investigating the reactivity of human anti-gg-1 antibodies, purified from 21 hsv-1-isolation-proven patient sera, to cellulose-bound synthetic peptides spanning the entire gg-1 sequence. the epitope mapping demonstrated that these antibo ... | 2000 | 10725430 |
| reactivation of genital herpes simplex virus type 2 infection in asymptomatic seropositive persons. | most persons who have serologic evidence of infection with herpes simplex virus (hsv) type 2 (hsv-2) are asymptomatic. historically, it has been assumed that these persons have less frequent viral reactivation than those with symptomatic infection. | 2000 | 10727588 |
| herpes simplex virus infections. new treatment approaches make early diagnosis even more important. | the herpes simplex viruses hsv-1 and hsv-2 cause problems for millions of people worldwide. infections range from simple cold sores and fever blisters to severe central nervous system disorders. development of effective antiviral medications has made prompt recognition important in primary care practice. appropriate therapy can significantly reduce both medical and psychosocial ramifications of herpes infections and can greatly improve the quality of life for many patients. | 2000 | 10728143 |
| herpes simplex virus virion host shutoff (vhs) activity alters periocular disease in mice. | during lytic infection, the virion host shutoff (vhs) protein of herpes simplex virus (hsv) mediates the rapid degradation of rna and shutoff of host protein synthesis. in mice, hsv type 1 (hsv-1) mutants lacking vhs activity are profoundly attenuated. hsv-2 has significantly higher vhs activity than hsv-1, eliciting a faster and more complete shutoff. to examine further the role of vhs activity in pathogenesis, we generated an intertypic recombinant virus (kosv2) in which the vhs open reading f ... | 2000 | 10729135 |
| persistence of infectious herpes simplex virus type 2 in the nervous system in mice after antiviral chemotherapy. | young adult mice were inoculated with herpes simplex virus type 2 (hsv-2) in the ear pinna. a relatively severe infection resulted, and 45% of the mice died by 11 days postinfection. therapy at 1 mg/ml by means of the drinking water with either famciclovir for periods of 5 or 10 days or valaciclovir for 5, 10, 15, or 20 days decreased clinical signs and reduced mortality to 15% or less. throughout a period of 27 days, mice were tested daily for the presence of infectious virus in the ear pinna, ... | 2000 | 10602729 |
| human immunodeficiency virus infection and genital ulcer disease in south africa: the herpetic connection. | while genital ulcers are a risk factor in hiv infection, the association of specific agents of genital ulcer disease (gud) with hiv infection may vary. | 2000 | 10654864 |
| herpes simplex type 2 infection in the developing world: is it time to address this disease? | 2000 | 10654865 | |
| medical care expenditures for genital herpes in the united states. | approximately 45 million americans have serologic evidence of hsv-2 infection and hsv-2 seroprevalence in the united states has increased 30% over the past two decades. despite rapid increases in hsv-2 prevalence, the last estimate of the u.s. national direct medical cost for genital herpes (gh) was completed in 1985. the objective of this study is to assess the u.s. direct medical expenditures for gh and its complications to assist policy makers in allocating limited std resources efficiently. | 2000 | 10654866 |
| diagnosis of herpes simplex virus infections in the clinical laboratory by lightcycler pcr. | herpes simplex virus (hsv) causes several clinical manifestations in both normal and immunocompromised hosts; this agent is the most frequently detected virus in diagnostic laboratories. recovery of the virus in cell culture is considered the "gold standard" for detection of this virus from sources other than cerebrospinal fluid. lightcycler is a newly developed, commercially available system designed to rapidly perform pcr, with real-time detection of pcr products by a fluorescence resonance en ... | 2000 | 10655387 |
| carrageenans from chilean samples of stenogramme interrupta (phyllophoraceae): structural analysis and biological activity. | carrageenans extracted from cystocarpic and tetrasporic stenogramme interrupta were analysed by chemical and spectroscopic methods. the carrageenan from cystocarpic plants is composed predominantly of 0.5 m kcl-insoluble and 1 m kcl-soluble fractions. the insoluble fraction contained iota-carrageenan as the major component with alpha-carrageenan and pyruvated carrageenan as minor components. the soluble fraction is highly heterogeneous and did not contain the precursors mu- and nu-carrageenans. ... | 2000 | 10656412 |
| psychosocial impact of type-specific herpes simplex serological testing on asymptomatic sexual health clinic attendees. | the usefulness of type-specific testing for herpes simplex virus type 2 (hsv-2) is much debated with proponents arguing for likely change in the sexual behaviour of asymptomatic carriers and opponents suggesting that testing may have significant adverse psychological impact while not promoting behaviour change. in the present study we examine the impact of hsv-2 serological testing on psychological well-being, self-esteem, anxiety, sexual self-perceptions and sexual practices among a sample of 1 ... | 2000 | 10667895 |
| poly(sodium 4-styrene sulfonate): an effective candidate topical antimicrobial for the prevention of sexually transmitted diseases. | presently marketed vaginal barrier agents are cytotoxic and damage the vaginal epithelium and natural vaginal flora with frequent use. novel noncytotoxic agents are needed to protect women from sexually transmitted diseases. one candidate compound is a high-molecular-mass form of soluble poly(sodium 4-styrene sulfonate) (t-pss). the antimicrobial activity of t-pss was evaluated in primary culture systems and in a genital herpes murine model. results obtained indicate that t-pss is highly effecti ... | 2000 | 10669374 |
| herpes simplex virus type 2 shedding in human immunodeficiency virus-negative men who have sex with men: frequency, patterns, and risk factors. | we investigated the frequency, site, and risk factors for herpes simplex virus (hsv) shedding in 30 human immunodeficiency virus (hiv)-negative hsv type 2 (hsv-2)-seropositive men who have sex with men. subjects collected daily hsv culture samples from genital, perianal, and oral areas for 100 days and maintained diaries of signs and symptoms. sixteen men (53.3%) shed hsv-2, and 9 (56.3%) of 16 men who were also hsv type 1 (hsv-1)-seropositive shed hsv-1. overall, hsv-2 was isolated on 3.1% of t ... | 2000 | 10671325 |
| asymptomatic herpes simplex virus shedding and russian roulette. | 2000 | 10671326 | |
| herpes simplex virus dna in amniotic fluid without neonatal infection. | twenty-one pregnant women were studied to determine the distribution of herpes simplex virus (hsv): 10 had symptomatic genital herpes, including 1 with primary cervical hsv infection, and 11 had asymptomatic genital herpes. samples from vesicles, the cervix, and amniotic fluid (af) were analyzed with 2 separate polymerase chain reaction (pcr) tests and with viral culture. for newborns, clinical examinations and pharyngeal hsv cultures were performed, and cord blood igm antibodies to hsv were mea ... | 2000 | 10671342 |
| viral causes of the acute retinal necrosis syndrome. | the primary goal of this study was to determine the viral cause of the acute retinal necrosis syndrome in 28 patients (30 eyes). a secondary goal was to investigate possible associations between viral cause and patient age, and viral cause and central nervous system disease. | 2000 | 10682968 |
| immunologic strategies for herpes vaccination. | 2000 | 10683051 | |
| immunologic strategies for herpes vaccination. | 2000 | 10683052 | |
| genital herpes and public health: addressing a global problem. | genital herpes can be caused by herpes simplex virus 2 (hsv-2) or, less commonly, by herpes simplex virus 1 (hsv-1). with a seroprevalence of antibodies to hsv-2 of 22% in the general population, genital herpes is 1 of the 3 most prevalent sexually transmitted diseases (stds) in the united states. a central issue in the public health problem of genital herpes is the high proportion of genital hsv infections that are unrecognized by both patients and clinicians. persons who are hsv-2 seropositive ... | 2000 | 10683059 |
| the cyclohexene ring system as a furanose mimic: synthesis and antiviral activity of both enantiomers of cyclohexenylguanine. | both enantiomers of cyclohexenylguanine were synthesized in a stereospecific way starting from the same starting material: r-(-)-carvone. both compounds showed potent and selective anti-herpesvirus activity (hsv-1, hsv-2, vzv, cmv). the binding of both cyclohexene nucleosides in the active site of hsv-1 thymidine kinase was investigated, and a model for the binding of both enantiomers is proposed. the amino acids involved in binding of the optical antipodes are the same, but the interaction ener ... | 2000 | 10691698 |
| in vitro and in vivo evaluations of sodium lauryl sulfate and dextran sulfate as microbicides against herpes simplex and human immunodeficiency viruses. | the efficacy of sodium lauryl sulfate (sls), a sulfated anionic chaotropic surfactant, and dextran sulfate (ds), a polysulfated carbohydrate, against herpes simplex virus (hsv) and human immunodeficiency virus (hiv) infections was evaluated in cultured cells and in different murine models of hsv infection. results showed that both sls and ds were potent inhibitors of the infectivities of various hsv-1 and hsv-2 strains. pretreatment of hiv-1 (strain nl4-3) with sls also reduced its infectivity t ... | 2000 | 10618073 |
| effect of route of vaccination with vaccinia virus expressing hsv-2 glycoprotein d on protection from genital hsv-2 infection. | to evaluate the effects of the route of vaccination on protection against genital hsv infection guinea pigs were vaccinated twice with vaccinia recombinants expressing hsv-2 glycoprotein d or controls by intradermal, intranasal or intravaginal inoculation. following hsv-2 intravaginal challenge, immunization by all 3 routes reduced vaginal hsv shedding and acute genital disease. the intravaginal vaccination, however, most effectively reduced primary and recurrent hsv disease. vaccinia and hsv an ... | 2000 | 10618532 |
| [alpha-herpes virus infections--male genital herpes]. | in japan, male genital herpes is the third popular male std, the most popular one being gonococcal infection and the second chlamydial infection. as specific clinical findings, superficial ulcer lesions with pain are formed in the genital area, especially in the prepuce. after hsv infection in genital mucocutaneous sites, viral particles are transported to the neurons. the state of subsequent hsv infection from external genitalia to the neurons is known as latent infection. 76% of our cases of t ... | 2000 | 10774209 |
| monitoring drug resistance for herpesviruses. | herpes simplex type 1 (hsv-1) and type 2 (hsv-2), varicella-zoster virus (vzv) and human cytomegalovirus (cmv) cause diseases that are usually self-limiting in the immunocompetent host. however, hsv-1, hsv-2, vzv, and cmv are major causes of morbidity and mortality in the immunocompromised patient. prolonged antiviral treatment is often required for the clinical management of herpesvirus infections in the immunocompromised patient, and this favors the emergence of drug-resistant strains. the iso ... | 2000 | 21331907 |
| a colorimetric microtiter plate polymerase chain reaction system that detects herpes simplex virus in cerebrospinal fluid and discriminates genotypes 1 and 2. | herpes simplex virus (hsv) is an ubiquitous agent responsible for a wide variety of human infections. in addition to epithelial infections such as gingivostomatitis, pharyngitis, genital herpes, whitlow, conjunctivitis, and keratitis, hsv is an important cause of central nervous system (cns) infections and accounts for 2-19% of human encephalitis cases (1,2). the clinical spectrum of cns diseases has been recently expanded; for example, most cases of benign recurrent aseptic meningitis (mollaret ... | 2001 | 21370153 |
| nested multiplex polymerase chain reaction for the diagnosis of cutaneous herpes simplex and herpes zoster infections and a comparison with electronmicroscopy. | herpes simplex virus (hsv) and varicella zoster virus (vzv) are common causes of cutaneous and mucocutaneous vesicular eruptions. laboratory diagnostic techniques include tzanck smears, electronmicroscopy, antigen detection and viral culture. this paper describes a nested multiplex polymerase chain reaction with respective sensitivities of 0.0001, 0.01 and 0.1 tcid50 for vzv, hsv-1 and hsv-2. the assay was used in (a) a salvage capacity for slides already processed for electronmicroscopy, and (b ... | 2001 | 11130887 |
| susceptibility to acyclovir of herpes simplex virus isolates obtained between 1977 and 1996 in japan. | the susceptibility of genital herpes to acyclovir (acv) in immunocompetent women was examined, as was the frequency of acv-resistant viruses by analyzing 56 clinical isolates in japan between 1977 and 1996. the mean susceptibilities of herpes simplex virus (hsv) type 1 and type 2 were 0.13+/-0.74 and 0.42+/-0.14 microg/ml, respectively, assessed by the 50% inhibitory concentration of plaque formation. the susceptibility to acv of clinical isolates did not changed since 1977, and also that of nin ... | 2001 | 11130888 |
| prospective study of pathogen burden and risk of myocardial infarction or death. | we previously demonstrated that the risk of coronary artery disease (cad) increased in relation to the number of pathogens (the "pathogen burden") in a cross-sectional study. in the present prospective study with a different patient cohort, we evaluated the effect of pathogen burden on the risk of myocardial infarction (mi) or death among cad patients. | 2001 | 11136684 |
| role of polymorphonuclear leukocytes in resolution of hsv-2 infection of the mouse vagina. | a naturally occurring population of polymorphonuclear leukocytes (pmns) was detected in the vaginal lumen of uninoculated mice. a large population of these cells also infiltrated the vaginal mucosa following intravaginal hsv-2 inoculation. we examined the role of pmns in preventing infection of the vaginal mucosa, virus clearance, and limiting virus spread to the lumbosacral ganglia. depletion of pmns prior to hsv-2 inoculation did not increase the incidence of infection suggesting that the smal ... | 2001 | 11137112 |
| differential roles of b cells and ifn-gamma-secreting cd4(+) t cells in innate and adaptive immune control of genital herpes simplex virus type 2 infection in mice. | the role of b, cd4(+) t and cd8(+) t cells in both primary genital infection with attenuated herpes simplex virus type 2 (hsv-2) and development of protective immunity to a later challenge with virulent hsv-2 using lymphocyte-deficient mice has been elucidated. following primary inoculation with attenuated thymidine kinase-deficient (tk(-)) hsv-2, b cell-deficient (micromt) mice developed a local viraemia and transient genital inflammation, suggesting a role for b cells in the innate control of ... | 2001 | 11257190 |
| induction of herpes simplex virus gb-specific cytotoxic t lymphocytes in tap1-deficient mice by genetic immunization but not hsv infection. | loading of most endogenous peptides on major histocompatibility complex class i molecules is conditional on their transport into the endoplasmic reticulum (er) by the peptide transporter tap. we describe an hsv-2/1 cross-reactive cytotoxic t-cell (ctl) epitope that is processed in a tap1-independent manner in vivo following immunization of tap1-/- mice with naked dna or a recombinant vaccinia virus. these data indicated that tap1-independent processing of endogenous proteins is sufficient to pri ... | 2001 | 11259190 |
| syntheses and structure--activity relationships of novel apio and thioapio dideoxydidehydronucleosides as anti-hcmv agents. | on the basis of the fact that apio dideoxynucleosides, in which the furanose oxygen and the c2 of the 2,3-dideoxyribose are transposed, exhibited potent anti-hiv activity and 2',3'-dideoxy-2',3'-didehydronucleosides also showed potent anti-hiv activity, we synthesized apio dideoxydidehydronucleosides in which the oxygen atom and the double bond of the 2,3-dideoxy-2,3-didehydroribose are exchanged. the thioapio dideoxydidehydronucleosides were also synthesized since sulfur serves as a bioisostere ... | 2001 | 11262090 |
| identification of herpes simplex virus genital infection: comparison of a multiplex pcr assay and traditional viral isolation techniques. | genital herpes simplex virus (hsv) is of major public health importance, as indicated by the marked increase in the prevalence of genital herpes over the past two decades. viral culture has traditionally been regarded as the gold standard for diagnosis. in this study, we compared viral culture and the amplification of hsv dna by the polymerase chain reaction (pcr) with respect to sensitivity, cost, clinical utility, and turnaround time. patient sample swabs from 100 individuals were inoculated o ... | 2001 | 11266519 |
| porcine cells are susceptible to infection by human alpha herpesviruses. | 2001 | 11267020 | |
| management of neonatal herpes simplex virus infection. | herpes simplex viruses (hsv) are ubiquitous pathogens which can be transmitted vertically causing significant morbidity and mortality in neonates. neonatal hsv infection is infrequent with an incidence ranging from 1 in 3,500 to 1 in 20,000, depending on the population. neonatal hsv infection is much more frequent in infants born to mothers experiencing a primary hsv infection with an incidence approaching 50%, while infants born to mothers experiencing recurrent hsv infection have an incidence ... | 2001 | 11269641 |
| sexually transmitted infections and cervicovaginal dysplasia in a cohort of human immunodeficiency virus-positive women in turin. | the correlation between sexually transmitted infections and cervicovaginal dysplasia has been evaluated in a cohort of 135 women who tested positive for human immunodeficiency virus type i (hiv-1) and were admitted to amedeo di savoia hospital of turin during the years 1997 and 1998 (stages b2 and b3 or c2 and c3). of these women. 31 presented with sexually transmitted diseases (stds; mean age, 33.5 +/- 5.9 years). among them, 14 were affected by cervicovaginal dysplasia of differing severity; h ... | 2001 | 11270419 |
| porcine hvec, a member of the highly conserved hvec/nectin 1 family, is a functional alphaherpesvirus receptor. | human herpesvirus entry mediator c (hvec) is an alphaherpesvirus receptor which binds to virion glycoprotein d (gd). we identified porcine hvec and studied its interaction with pseudorabies virus (prv) and herpes simplex virus type 1 (hsv-1) gd. porcine and human hvec have 96% amino acid identity and hvec from african green monkey, mouse, hamster, and cow are similarly conserved. porcine hvec mediates entry of hsv-1, hsv-2, prv, and bovine herpesvirus type 1. truncated soluble forms of hsv-1 and ... | 2001 | 11277703 |
| herpesvirus infection of seborrheic keratoses. | we present three examples of patients with seborrheic keratoses complicated by necrotizing herpesvirus infection. two patients had localized cutaneous herpetic infections, and the third patient had a generalized cutaneous herpesvirus infection. two of the lesions were thought to be squamous cell carcinoma. the third was clinically identified as inflamed seborrheic keratosis. herpesvirus infection was not clinically suspected in two of the patients. the histologic changes were similar in all case ... | 2001 | 11285412 |
| herpes simplex virus type 1 entry is inhibited by the cobalt chelate complex ctc-96. | the ctc series of cobalt chelates display in vitro and in vivo activity against herpes simplex virus types 1 and 2 (hsv-1 and hsv-2). the experiments described here identify the stage in the virus life cycle where ctc-96 acts and demonstrate that the drug inhibits infection of susceptible cells. ctc-96 at 50 microg/ml has no effect on adsorption of virions to vero cell monolayers. penetration assays reveal that ctc-96 inhibits entry of the virus independent of gc and cellular entry receptors. th ... | 2001 | 11287561 |
| prevalence and risk factors of hsv-1 and hsv-2 antibodies in european hiv infected women. | to investigate the prevalence and risk factors of hsv-1 and hsv-2 antibodies in hiv infected women and the association between recurrent genital ulcerations and hiv disease progression in hsv-2 positive women. | 2001 | 11287691 |
| human papillomaviruses and cervical cancer in bangkok. i. risk factors for invasive cervical carcinomas with human papillomavirus types 16 and 18 dna. | personal interviews, tests for antibodies to herpes simplex virus type 2, treponema pallidum, and hepatitis b, tests for hepatitis b surface antigen (hbsag), and polymerase chain reaction-based assays for human papillomavirus (hpv) dna in cervical scrapings were obtained from 190 women with squamous cell and 42 women with adenomatous cervical carcinoma and from 291 hospitalized controls diagnosed in bangkok, thailand, between september 1991 and september 1993. risk was strongly associated with o ... | 2001 | 11296143 |
| synthesis of 1-(2-deoxy-beta-d-ribofuranosyl)-2,4-difluoro-5-substituted-benzene thymidine mimics, some related alpha-anomers, and their evaluation as antiviral and anticancer agents. | a group of unnatural 1-(2-deoxy-beta-d-ribofuranosyl)-2,4-difluorobenzenes having a variety of c-5 substituents (h, me, f, cl, br, i, cf3, cn, no2, nh2), designed as thymidine mimics, were synthesized for evaluation as anticancer and antiviral agents. the coupling reaction of 3,5-bis-o-(p-chlorobenzoyl)-2-deoxy-alpha-d-ribofuranosyl chloride with an organocadmium reagent [(2,4-difluorophenyl)2cd] afforded a mixture of the alpha- and beta-anomeric products (alpha:beta = 3:1 to 10:1 ratio). treatm ... | 2001 | 11303557 |
| synthesis of 1-(2-deoxy-beta-d-ribofuranosyl)-2,4-difluoro-5-substituted-benzenes: "thymine replacement" analogs of thymidine for evaluation as anticancer and antiviral agents. | a group of unnatural 1-(2-deoxy-beta-d-ribofuranosyl)-2,4-difluorobenzenes having a variety of c-5 two-carbon substituents [-c...c-x, x = i, br; -c...ch; (e)-ch=ch-x, x = i, br; -ch=ch2; -ch2ch3; -ch(n3) ch2br], designed as nucleoside mimics, were synthesized for evaluation as anticancer and antiviral agents. the 5-substituted (e)-ch=ch-i and -ch2ch3 compounds exhibited negligible cytotoxicity in a mtt assay (cc50 = 10(-3) to 10(-4)m range), relative to thymidine (cc50 = 10(-3) to 10(-5)m range) ... | 2001 | 11303562 |
| [intrauterine herpes simplex virus infection]. | early fetal herpes simplex virus (hsv) infection is rarely documented. only the minority of affected fetuses survive this condition. | 2001 | 11305194 |
| antitumor agents. 207. design, synthesis, and biological testing of 4beta-anilino-2-fluoro-4'-demethylpodophyllotoxin analogues as cytotoxic and antiviral agents. | 2-fluoropodophyllotoxin (11) and several 4beta-anilino-2-fluoro-4'-o-demethyl analogues were synthesized and evaluated in both antineoplastic and antiviral assays. these compounds were moderately active against some cancer cell lines, but they were less active than the corresponding nonfluorinated analogues. compound 11 exhibited the best activity against kb carcinoma with a gi(50) of approximately 30 nm. most compounds exhibited moderate activity against hcmv with id(50) and id(90) values in th ... | 2001 | 11311065 |
| prevalence and risk factors for herpes simplex virus type 2 infection among middle-age women in brazil and the philippines. | data comparing type-specific herpes simplex virus type 2 (hsv-2) seroprevalence and risk factors between comparable populations are largely unavailable, particularly from less-developed countries. | 2001 | 11318248 |
| herpes simplex virus type 2 as a cause of acute retinal necrosis syndrome in young patients. | to determine the causative virus in acute retinal necrosis (arn) syndrome in a series of patients by calculation of modified witmer coefficients. | 2001 | 11320015 |
| the burden of reproductive-organ disease in rural women in the gambia, west africa. | data on the epidemiology of reproductive-organ morbidity are needed to guide effective interventions, to set health-care priorities, and to target future research. this study aimed to find out the prevalence of reproductive-organ disease in a sample of rural gambian women. | 2001 | 11323043 |
| incidence of herpes simplex virus type 2 infection in the united states. | between the time that two large, national surveys were conducted, the second national health and nutrition examination survey (1976-1980) and the third national health and nutrition examination survey (1988-1994), prevalence of herpes simplex virus type 2 (hsv-2) infection in the united states increased by 30%. from these survey data, the authors estimated the incidence of hsv-2 infection in the civilian, noninstitutionalized population aged > or = 12 years by means of a mathematical model that ... | 2001 | 11323323 |
| effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in balb/c mice. | to study the effect of aminoguanidine (amg), an inhibitor of nitric oxide production, on the ocular infection of balb/c mice with herpes simplex virus (hsv) type 1 strain f and hsv-2 strain g. | 2001 | 11328740 |