Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| is the presence of trichomonas vaginalis a reliable predictor of coinfection with chlamydia trachomatis and/or neisseria gonorrhoeae in female ed patients? | the aim of the study was to determine if the presence of trichomonas vaginalis (tv) is associated with an increased risk of coinfection with chlamydia trachomatis (ct) and/or neisseria gonorrhoeae (ng) in female patients presenting to the ed with symptoms consistent with a sexually transmitted disease (std). | 2005 | 15765329 |
| synthesis and antiprotozoan evaluation of new alkyl-linked bis(2-thioxo-[1,3,5]thiadiazinan-3-yl) carboxylic acids. | two new series of several alkyl-linked bis(2-thioxo-[1,3,5]thiadiazinan-3-yl) carboxylic acids were synthesized in a two step procedure from the corresponding alkyl bis-dithiocarbamic salt intermediary. the novel compounds were evaluated for their activity in vitro against trypanosoma cruzi strain cl (clone cl b5) and trichomonas vaginalis strain jh 31a. | 2005 | 15848753 |
| kinetics of immunoglobulin g, m, a and igg subclass responses in experimental intravaginal trichomoniasis: prominence of igg1 response. | trichomoniasis, the most common nonviral sexually transmitted disease, is caused by infection with the protist trichomonas vaginalis. the clinical spectrum varies from an asymptomatic state to mild, moderate or severe symptoms. however, the exact factors leading to varied symptomatology have not been well elucidated. the mouse is a useful experimental model for intravaginal trichomoniasis, for understanding the role of local immune responses in the pathogenesis and varied symptomatology of this ... | 2005 | 16255745 |
| rifaximin, a peculiar rifamycin derivative: established and potential clinical use outside the gastrointestinal tract. | rifaximin is a poorly absorbed semisynthetic rifamycin derivative with a broad spectrum of antibacterial activity including gram-positive and gram-negative bacteria, both aerobes and anaerobes. although originally developed for the treatment of infectious diarrhea, the appreciation of the pathogenic role of gut bacteria in several organic and functional gastrointestinal diseases has increasingly broadened its clinical use. the availability of a topical formulation (a cream containing 5% of the d ... | 2005 | 15855757 |
| trichomonas vaginalis: identification of soluble and membrane-associated phospholipase a1 and a2 activities with direct and indirect hemolytic effects. | a direct hemolytic activity, dependent on phospholipase a (pla) activity, was located in the particulate subcellular fraction (p30) of trichomonas vaginalis. we identified soluble direct and indirect hemolytic activities in the spent medium and soluble fraction (s30) of t. vaginalis strain gt-13. spent medium showed the highest specific indirect hemolytic activity (siha) at ph 6.0 (91 indirect hemolytic units [hu]/mg/hr). spent medium and p30, but not s30, showed direct hemolytic activity. pla a ... | 2005 | 15856864 |
| measuring the significance of field validation in the college of american pathologists interlaboratory comparison program in cervicovaginal cytology: how good are the experts? | expert opinion is often used as a gold standard for gynecologic cytology in the evaluation of new technologies, in the legal setting, and in the validation of cases for use in educational programs and proficiency testing. however, the reliability of expert opinion alone in selecting slides of a specific cytodiagnosis that can be reproducibly and reliably identified by subsequent reviewers has not been determined. | 2005 | 15859630 |
| increased sensitivity of trichomonas vaginalis isolation from vaginal secretions by subsequent blind passage of preliminary negative cultures. | trichomonas vaginalis is a vaginal protozoon causing the most prevalent nonviral sexually transmitted disease. culture is considered as the most reliable and gold standard among conventional diagnostic methods in the detection of t. vaginalis. in the present study, the diagnostic effect of subsequent blind culturing of vaginal secretion samples in diamond's complete medium at every 48 h was investigated in 93 women. vaginal secretion samples were examined by wet mount and inoculated into diamond ... | 2005 | 15878448 |
| the diagnosis of trichomonas vaginalis in liquid-based pap tests: correlation with pcr. | the conventional papanicolaou smear (cps) is not considered accurate for the diagnosis of trichomonas vaginalis (t. vaginalis), and women noted to carry the organism on cps are recommended to undergo confirmatory testing. liquid-based preparations have been shown to facilitate the diagnosis of squamous lesions and may also facilitate the diagnosis of t. vaginalis. we used polymerase chain reaction (pcr) to investigate the accuracy of the diagnosis of t. vaginalis by the liquid-based pap test (lb ... | 2005 | 15880709 |
| prevalence of trichomonas vaginalis infection in and around surat. | trichomonas culture method was used for the diagnosis of trichomoniasis along with gram staining and wet mount preparation. we selected 51 urban women and 51 rural women in this study. samples were taken from both symptomatic and asymptomatic individuals. out of 102 samples 35 samples were positive for trichomonas vaginalis by culture method. 20 women were positive for trichomonas vaginalis by wet mount and 15 by gram staining technique. so it is a fact that culture of trichomonas vaginalis is a ... | 2005 | 16366122 |
| sexually transmitted infections among female sex workers in yunnan, china. | a cross-sectional prevalence survey of sexually transmitted infections (stis) was conducted among female sex workers (fsws) in kunming, yunnan province, china. a total of 505 fsws participated in the survey. all eligible participants gave informed consent. demographic, behavioral, and clinical information of the participants was gathered by direct structured interviews. tampon swabs were collected to test for chlamydia trachomatis, nesseria gonorrhoeae, and trichomonas vaginalis and serum specim ... | 2005 | 16375618 |
| premature with trichomoniasis: report of one case. | we report a case of an extremely-low-birth-weight premature infant with trichomonas vaginalis infection of the vagina and urinary tract. her mother is also infected with trichomonas vaginalis but is asymptomatic. the patient's illness started as an asymptomatic pyuria and later on at early infancy developed profuse malodorous vaginal discharge. her vaginal discharge was positive for trichomonas vaginalis, both on wet mount and papanicolaou smear, and the infection responded well to treatment wit ... | 2005 | 16381336 |
| sexually transmitted infections, blood-borne viruses and risk behaviour in an australian senior high school population--the shlirp study. | to determine the feasibility and acceptability of screening for sexually transmitted infections and blood-borne viruses and to study the profile of sexual activity and other risk behaviours in a senior high school population. | 2005 | 16402670 |
| bacterial vaginosis in female sex workers in chennai, india. | bacterial vaginosis (bv) causes obstetric and gynaecological complications and non-chlamydial/non-gonococcal pelvic inflammatory disease and has been shown to be associated with the risk of acquiring hiv and herpes simplex (hsv)-2 infections. this study investigated both the prevalence of bv and its association with stds among 582 female sex workers living in chennai, south india. blood, vaginal and endocervical swabs were tested for hsv-2, hiv, treponema pallidum, bv, chlamydia trachomatis, nei ... | 2005 | 16402677 |
| [the implication of trichomoniasis and giardiasis in the development of inflammatory diseases of small pelvic organs]. | 2005 | 16445233 | |
| microbicidal spermicide or spermicidal microbicide? | vaginal contraception, the oldest method of fertility regulation that remained virtually forgotten for a few decades has recently come under focal review due to an increase in stds and hiv infections worldwide. today it is being considered very strongly that a conceptual microbicidal spermicide can tender protection against pregnancy as well as stds (including aids), simultaneously. however the two activities (spermicidal and microbicidal) need to be integrated in vaginal preparations, as many w ... | 2005 | 16448947 |
| [rna interference to the expression of peroxiredoxin-related genes in trichomonas vaginalis]. | to inhibit the expression of the target genes of peroxiredoxin (prx) and thioredoxin reductase (trxr) by rna interference and evaluate its effect on the growth of trichomonas vaginalis. | 2005 | 16566216 |
| trichomonas vaginalis: paradigm of a successful sexually transmitted organism. | trichomonas vaginalis (tv) is one of the most successful protozoan pathogens and one of the most common sexually transmitted organism in females, yet it is also one of the most poorly investigated. by producing a wide array of glycosidases and cysteine proteinase enzymes, the organism can easily adapt to the environment, harvesting host proteins and dna for metabolism. with the ability to cause lesions, vaginitis and acute inflammatory disease of the genital mucosa, tv acts as a potential cataly ... | 2005 | 16411380 |
| [exogenous and endogenous infections of genital tract]. | the paper is an overview of etiological factors, clinical manifestations, diagnostics and treatment of genital tract infections. infections caused by trichomonas vaginalis, fungi candida, saccharomyces and geotrichum are discussed as well as syphilis, gonorrhea and infections with chlamydia, mycoplasms and ureaplasms. the problem of vaginal dysbacteriosis and mixed infections is pointed out. | 2005 | 16463706 |
| phylogenetic positions of several amitochondriate protozoa--evidence from phylogenetic analysis of dna topoisomerase ii. | several groups of parasitic protozoa, as represented by giardia, trichomonas, entamoeba and microsporida, were once widely considered to be the most primitive extant eukaryotic group--archezoa. the main evidence for this is their 'lacking mitochondria' and possessing some other primitive features between prokaryotes and eukaryotes, and being basal to all eukaryotes with mitochondria in phylogenies inferred from many molecules. some authors even proposed that these organisms diverged before the e ... | 2005 | 16483135 |
| structure of citrobacter freundii l-methionine gamma-lyase. | l-methionine gamma-lyase (mgl) is a pyridoxal 5'-phosphate (plp) dependent enzyme that catalyzes gamma-elimination of l-methionine. the crystal structure of mgl from citrobacter freundii has been determined at 1.9 a resolution. the spatial fold of the protein is similar to those of mgls from pseudomonas putida and trichomonas vaginalis. the comparison of these structures revealed that there are differences in plp-binding residues and positioning of the surrounding flexible loops. | 2005 | 16511092 |
| [incidence in manisa of trichomonas vaginalis in women with a vaginal discharge.] | vaginal discharge caused by trichomonas vaginalis is one of the most frequently encountered sexually transmitted infections in women. this study was planned to investigate the frequency of t. vaginalis in patients with a vaginal discharge. vaginal specimens were collected from 233 women with a vaginal discharge and from 100 women without any discharge as a control group. materials were taken with a speculum and sterile cotton swabs from the posterior fornix of the vagina; and inoculated into try ... | 2005 | 17167734 |
| prevalence and determinants of genital shedding of herpes simplex virus among women attending italian colposcopy clinics. | to determine the prevalence of herpes simplex virus (hsv) shedding among women attending italian colposcopy clinics and describe their lifestyle, demographic characteristics, genital symptoms and signs. | 2005 | 15596279 |
| evaluation of a cognitive-behavioral, group, randomized controlled intervention trial to prevent sexually transmitted infections and unintended pregnancies in young women. | few cognitive-behavioral interventions have focused on preventing sexually transmitted infections (stis) and unintended pregnancies (ups) in young, sexually active women in a single study. military recruit training provides a well-defined, national, nonclinic sample in which to evaluate such an intervention. | 2005 | 15530594 |
| a potentially functional mariner transposable element in the protist trichomonas vaginalis. | mariner transposable elements encoding a d,d34d motif-bearing transposase are characterized by their pervasiveness among, and exclusivity to, animal phyla. to date, several hundred sequences have been obtained from taxa ranging from cnidarians to humans, only two of which are known to be functional. related transposons have been identified in plants and fungi, but their absence among protists is noticeable. here, we identify and characterize tvmar1, the first representative of the mariner family ... | 2005 | 15371525 |
| hela cell nucleus, a source of thymidine for trichomonas vaginalis growing in vitro. | trichomonas vaginalis is a parasitic protist incapable of de novo purine and pyrimidine biosynthesis. the lack of these de novo syntheses of nucleotides is supplemented with purine and pyrimidine salvage pathways. likewise, t. vaginalis is incapable of converting its ribonucleotides to deoxyribonucleotides. therefore, the parasite must rely on the salvage of exogenous deoxyribonucleosides for dna synthesis. it has been demonstrated that the parasite can incorporate external adenine and guanine i ... | 2005 | 15381159 |
| [vulvovaginitis: vaginal ph changes and associated microflora]. | to establish a correlation between ph vaginal and the microflora associated in carriers of vulvovaginites. | 2005 | 17768818 |
| [evaluation of an agglutination method with latex particles sensitised for the diagnosis of vaginal trichomoniasis]. | a latex agglutination method for the detection of vaginal trichomoniasis, designed at the parasitology department of the national center of farming and stockbreeding health (cnsa, in spanish), was evaluated. 240 samples of vaginal exudates from women that visited the microbiology department of "10 de octubre" maternal and children's hospital, were analysed. this method was compared to the conventional direct examination used in the laboratories, taking the culture in diamond medium as an accurat ... | 2005 | 17966583 |
| the laboratory diagnosis of trichomonas vaginalis. | trichomonas vaginalis, a parasitic protozoa that causes the sexually transmitted infection trichomoniasis, is the sexually transmitted infection with the largest annual incidence, exceeding 170 million cases per year. the disease can be difficult to diagnose due to its heterogeneous presentation and problems with diagnostic testing. all diagnostic tests are fraught with imperfections, but the old, reliable wet mount examination (in trained hands), and the newer inpouch method may be advantageous ... | 2005 | 18159526 |
| screening for sexually transmitted infection pathogens in semen samples. | the transmission of sexually transmitted infection (sti) pathogens from an infected donor to the recipient of a semen donation in assisted conception may result not only in acute infection but also in long-term reproductive complications or adverse outcomes of pregnancy, including infection of the offspring. screening for bacterial sti pathogens, chlamydia trachomatis and neisseria gonorrhoeae is strongly recommended because these pathogens can cause serious reproductive complications in the rec ... | 2005 | 18159531 |
| comparative protein structure modeling using modeller. | functional characterization of a protein sequence is one of the most frequent problems in biology. this task is usually facilitated by accurate three-dimensional (3-d) structure of the studied protein. in the absence of an experimentally determined structure, comparative or homology modeling can sometimes provide a useful 3-d model for a protein that is related to at least one known protein structure. comparative modeling predicts the 3-d structure of a given protein sequence (target) based prim ... | 2006 | 18428767 |
| [the role of chlamydia trachomatis and some other associated microorganisms in endocervicitis]. | the objective of the study was to examine the prevalence of chlamydia trachomatis as a causative agent in women with clinically manifested endocervicitis and to find the age group where chlamydia trachomatis was most prevalent. 553 women sexually active women in reproductive ag with clinically manifested endocervicitis e were examined. chlamydial infection by direct immmunofluorescence was found in 239 (43, 2%) about 75% of the patients with chlamydial infection were in the age group 20-29 years ... | 2006 | 17168494 |
| trichomonas vaginalis and giardia intestinalis produce nitric oxide and display no-synthase activity. | 2006 | 17169054 | |
| hydrogenosomal activity of trichomonas vaginalis cultivated under different iron conditions. | to evaluate whether iron concentration in tym medium influence on hydrogenosomal enzyme gene expression and hydrogenosomal membrane potential of trichomonas vaginalis, trophozoites were cultivated in irondepleted, normal and iron-supplemented tym media. the mrna of hydrogenosomal enzymes, such as pyruvate ferredoxin oxidoreductase (pfor), hydrogenase, ferredoxin and malic enzyme, was increased with iron concentrations in t. vaginalis culture media, measured by rt-pcr. hydrogenosomal membrane pot ... | 2006 | 17170580 |
| failure to detect urethral trichomonas vaginalis in japanese men with or without urethritis. | trichomonas vaginalis may cause symptomatic or asymptomatic urethritis in men. there are few recent studies on the prevalence of t. vaginalis infection in japanese men, and quantification of the number of cases of urethritis attributable to this pathogen has not been performed in japan. the aim of this study was to determine the prevalence and morbidity of t. vaginalis infection in japanese men. | 2006 | 17083395 |
| high incidence of new sexually transmitted infections in the year following a sexually transmitted infection: a case for rescreening. | studies show 11% to 15% of women treated for chlamydia trachomatis are reinfected 3 to 4 months after treatment, suggesting the need for rescreening. there is little information on infections among men, infections with neisseria gonorrhoeae or trichomonas vaginalis, or long-term follow-up. | 2006 | 17043338 |
| dealing with trichomoniasis. | trichomoniasis is a common but less well known sexually transmitted infection affecting men and women. in men it is often asymptomatic and goes undetected. in women it can produce a profuse, frothy, unpleasant-smelling vaginal discharge with pruritus and soreness which is sometimes confused with vulvo-vaginal candidiasis (thrush) and bacterial vaginosis. women often mistakenly treat themselves for thrush with no result. diagnosis is by laboratory culture and treatment is with metronidazole. part ... | 2006 | 17139977 |
| vulnerability and risk factors for sexually transmitted infections and hiv among adolescents in kampala, uganda. | three hundred and six sexually experienced adolescents participated in a study on sexually transmitted infection (sti) prevalence and associated risk factors. the prevalence of neisseria gonorrhoea (ng), chlamydia trachomatis (ct), trichomonas vaginalis (tv) and syphilis was 4.5%, 9%, 8% and 4% for females and 4.7%, 5.7%, 0% and 2.8% for males. hiv-seropositivity was found in 15.2% of females and 5.8% of males. structured face-to-face interviews were used to obtain information about social backg ... | 2006 | 16971279 |
| methods for detection of trichomonas vaginalis in the male partners of infected women: implications for control of trichomoniasis. | trichomonas vaginalis infection in men is an important cause of nongonococcal urethritis. effective detection of the parasite in men using culture requires examination of multiple specimens. we compared culture and pcr-enzyme-linked immunosorbent assay in urethral swabs, urine, and semen for t. vaginalis detection in male sexual partners of women with trichomoniasis identified by wet mount and culture. trichomonads were detected by at least one positive test in 205/280 men (73.2%) who submitted ... | 2006 | 16971646 |
| identification and biochemical characterization of serine hydroxymethyl transferase in the hydrogenosome of trichomonas vaginalis. | serine hydroxymethyl transferase (shmt) is a pyridoxal phosphate (plp)-dependent enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and methylenetetrahydrofolate. we have identified a single gene encoding shmt in the genome of trichomonas vaginalis, an amitochondriate, deep-branching unicellular protist. the protein possesses a putative n-terminal hydrogenosomal presequence and was shown to localize to hydrogensomes by immunofluorescence analysis, providing ... | 2006 | 16980404 |
| trichomonas vaginalis lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells. | trichomonas vaginalis is one of the most common nonviral sexually transmitted human infections and, worldwide, has been linked to increased incidence of human immunodeficiency virus type 1 transmission, preterm delivery, low birth weight, cervical cancer, and vaginitis. the molecular pathways that are important in initiating host inflammatory and immune responses to t. vaginalis are poorly understood. here we report interactions of human cervicovaginal epithelial cells with the most abundant cel ... | 2006 | 16988255 |
| prevalence of trichomonas vaginalis isolates with resistance to metronidazole and tinidazole. | clinical isolates of trichomonas vaginalis were obtained from women consecutively enrolled in a study of partner notification. testing of susceptibility to metronidazole and tinidazole was performed. resistance to metronidazole and tinidazole was present in 17/178 (9.6%) and 1/178 (0.56%) strains, respectively. in vitro resistance was poorly correlated with clinical response to treatment. | 2006 | 17000740 |
| iron modulates ecto-phosphohydrolase activities in pathogenic trichomonads. | the presence of iron in the extracellular medium is essential for both in vivo and in vitro survival of pathogenic microorganisms, including trichomonas vaginalis and tritrichomonas foetus. in these parasites, iron is directly involved in the proliferation, protein expression and activation of critical enzymes. the purpose of this study was to investigate the role of iron in ecto-atpase, ecto-phophatase and secreted phosphatase activities of these trichomonads. we observed that trichomonads grow ... | 2006 | 17010660 |
| etiologic agents of cervicovaginitis in turkish women. | to investigate the distribution of microbiologic agents causing cervicovaginitis. | 2006 | 17013472 |
| [molecular cloning and characterization of a rras homologue gene from trichomonas vaginalis]. | to clone and characterize a rras-like gene from trichomonas vaginalis for studying cellular signal transduction pathways in the organism. | 2006 | 17094627 |
| [construction of prokaryotic expression vector for trichomonas vaginalis silent information regulator 2 and its expression]. | total rna was isolated from trichomonas vaginalis and tv-sir2-like cdna was amplified by rt-pcr and cloned into pgem-t easy plasmid. a fragment of tv-sir2-like cdna was subcloned into the expression vector pet-41b and expressed in e.coli bl21 with induction of iptg. the full-length of tv-sir2-like cdna was cloned and sequenced. the prokaryotic expression system of pet-41b/tv-sir2-like was constructed. the fusion protein of tv-sir2-like was expressed in e. coli bl21, occupying 30% of the total ba ... | 2006 | 17094634 |
| [investigation of the prevalence of trichomonas vaginalis with respect to diverse social variables in women (malatya, turkey)]. | trichomonas vaginalis is a monoxen parasite and its definitive host is human. it is known that the way of life is an important factor affecting incidence because of sexual transmission. in the present study, the incidence of t. vaginalis has been investigated with direct microscopy, giemsa staining and culture (cysteine-peptone-liver-maltose (cplm)). the study included 675 females with complaints of vaginal discharge who presented at different health departments or hospitals in the malatya regio ... | 2006 | 17106846 |
| [investigation of the prevalence of trichomonas vaginalis in women with complaints of vaginal discharge and itching]. | trichomonas vaginalis (t. vaginalis) is the most common pathogen that is sexually spread in women. in this study, the presence of trichomonas vaginalis was investigated in patients with vaginal discharge and itching who presented at the polyclinic of the mustafa kemal university medical faculty department of gynecology. these women were between 20-40 years of age. during gynecologic examination of patients, vaginal fluid samples were collected with swaps from the speculum and fornics to two tube ... | 2006 | 17106847 |
| [investigation of trichomonas vaginalis in patients with nonspecific vaginal discharge]. | trichomoniasis, a protozoan infection, has a worldwide distribution and is the most common nonviral, sexually transmitted disease. in this study, we examined the vaginal swab samples of 114 patients who presented at the obstetrics and gynecology outpatient clinic in ankara in 2004, for the presence of trichomonas vaginalis (t. vaginalis) using direct microscopy and culture methods. the samples were taken from patients with nonspecific vaginal discharge during their gynecological examination. twe ... | 2006 | 17106848 |
| bacterial vaginosis in women of low socioeconomic status living in slum areas in chennai, india. | bacterial vaginosis (bv) is a common cause of vaginitis among women of childbearing age. this study was performed to investigate the prevalence of bv and its association with sexually transmitted infections among 487 women of low socioeconomic status. blood, vaginal and endocervical swabs were tested for hiv, herpes simplex virus-2 (hsv-2), treponema pallidum, bv, chlamydia trachomatis, neisseria gonorrhoea and trichomonas vaginalis. of the women screened for bv, 120 (25, 95% ci 20.8-28.4) were ... | 2006 | 17112444 |
| the syndromic management of vaginal discharge using single-dose treatments: a randomized controlled trial in west africa. | to evaluate whether single-dose treatments are as effective as standard therapy in the syndromic management of vaginal discharge. | 2006 | 17128343 |
| association of genital shedding of herpes simplex virus type 2 and hiv-1 among sex workers in rural zimbabwe. | herpes simplex virus type 2 (hsv-2) facilitates sexual acquisition of hiv-1 but data on transmission are less clear. in this study the interaction between genital shedding of hiv-1 and hsv-2 was explored among zimbabwean sex workers. | 2006 | 16511420 |
| split-sample analysis of discarded cells from liquid-based pap smear sampling devices. | to examine cells that were retained on sampling devices used to collect thinprep (cytyc corp., boxborough, massachusetts, u.s.a) pap smears in order to evaluate both the number and significance of cells that are routinely discarded with these devices after liquid-based specimens are collected. | 2006 | 16514841 |
| hydrogenosomal succinyl-coa synthetase from the rumen-dwelling fungus neocallimastix patriciarum; an energy-producing enzyme of mitochondrial origin. | hydrogenosomes are hydrogen-producing organelles that are related to mitochondria and found in a variety of evolutionarily unrelated anaerobic microbial eukaryotes. similar to classic mitochondria, hydrogenosomes contain the enzyme catalyzing the only reaction of the citric acid cycle directly producing energy; succinyl-coa synthetase. we have isolated and characterized the genes encoding both subunits of this enzyme from the anaerobic chytrid fungus neocallimastix patriciarum, a model organism ... | 2006 | 16515848 |
| fe-hydrogenase maturases in the hydrogenosomes of trichomonas vaginalis. | assembly of active fe-hydrogenase in the chloroplasts of the green alga chlamydomonas reinhardtii requires auxiliary maturases, the s-adenosylmethionine-dependent enzymes hydg and hyde and the gtpase hydf. genes encoding homologous maturases had been found in the genomes of all eubacteria that contain fe-hydrogenase genes but not yet in any other eukaryote. by means of proteomic analysis, we identified a homologue of hydg in the hydrogenosomes, mitochondrion-related organelles that produce hydro ... | 2006 | 16524912 |
| nitric oxide radicals in leucocytes and vaginal washes of trichomonas vaginalis -infected symptomatic and asymptomatic women. | the clinical spectrum of trichomonas vaginalis infection varies from asymptomatic to mild, moderate or severe vaginitis. nitric oxide and other reactive nitrogen radicals produced by immune effector cells are important cytotoxic and cytostatic mediators against several microorganisms including parasites. in the present study, inducible nitric oxide synthase (inos) and reactive nitrogen intermediates (rni) were determined in leucocyte cultures (stimulated with t. vaginalis in vitro) and vaginal w ... | 2006 | 16529664 |
| patient-delivered partner treatment for trichomonas vaginalis infection: a randomized controlled trial. | infections with trichomonas vaginalis (tv) are common and recurrence rates are high. better methods of treating partners of women with trichomoniasis are needed. | 2006 | 16531939 |
| evolution of four gene families with patchy phylogenetic distributions: influx of genes into protist genomes. | lateral gene transfer (lgt) in eukaryotes from non-organellar sources is a controversial subject in need of further study. here we present gene distribution and phylogenetic analyses of the genes encoding the hybrid-cluster protein, a-type flavoprotein, glucosamine-6-phosphate isomerase, and alcohol dehydrogenase e. these four genes have a limited distribution among sequenced prokaryotic and eukaryotic genomes and were previously implicated in gene transfer events affecting eukaryotes. if our pr ... | 2006 | 16551352 |
| sexually transmitted infections among pregnant women attending an antenatal clinic in fuzhou, china. | to determine the prevalence of sexually transmitted infections (stis) among women attending an antenatal clinic in urban china and to show whether reported symptoms and findings on clinical examination predicted stis in this population. | 2006 | 16554698 |
| synthesis of benzenepropanamine analogues as non-detergent spermicides with antitrichomonas and anticandida activities. | fifteen analogues of benzenepropanamine were synthesized and evaluated for their spermicidal as well as microbicidal activities against trichomonas vaginalis and candida spp. several compounds showed appreciable dual activities. compound 12 exhibited good spermicidal (mec=0.1%) along with substantial anticandidal (mic=0.05%) activities, while compounds 3 and 6 showed significant microbicidal activities with moderate spermicidal effect. the sar of these structures is being discussed here in this ... | 2006 | 16793275 |
| trichomonas vaginalis and trichomoniasis in the republic of korea. | vaginal trichomoniasis, caused by trichomonas vaginalis, is the most common sexually transmitted disease. more than 170 million people worldwide are annually infected by this protozoan. in the republic of korea, 10.4% of women complaining of vaginal symptoms and signs were found to be infected with t. vaginalis. however, despite its high prevalence, the pathogenesis of t. vaginalis infection has not been clearly characterized although neutrophil infiltration is considered to be primarily respons ... | 2006 | 16809958 |
| sociodemographic markers and behavioral correlates of sexually transmitted infections in a nonclinical sample of adolescent and young adult women. | we examined sociodemographic markers and 3-month behavioral correlates of sexually transmitted infections (stis) in a nonclinical cross-section of adolescent and young adult women. | 2006 | 16826478 |
| is the presence of trichomonas on a pap smear associated with an increased incidence of bacterial vaginosis? | bacterial vaginosis (bv) is associated with significant morbidity. bacterial vaginosis is due to an overgrowth of anaerobic organisms in the vagina. it has been postulated that the presence of trichomonas creates an anaerobic environment that favors bv. thus, bv should be more frequent in the presence of trichomonas if trichomonas is creating a favorable anaerobic environment. | 2006 | 16829751 |
| symbiosis of mycoplasma hominis in trichomonas vaginalis may link metronidazole resistance in vitro. | fourteen of 28 trichomonas vaginalis isolates collected from patients in guangzhou, china from 2003 to 2004 were found to be naturally infected with mycoplasma hominis, as determined by pcr using specific primers. in vitro metronidazole sensitivity assay of the 28 isolates revealed four displaying low susceptibility [minimum lethal concentration (mlc)= approximately 13-25 microg/ml] and another four displaying high resistance (mlc=50-100 microg/ml). the overwhelming majority of these resistant i ... | 2006 | 16847608 |
| association of bacterial vaginosis, trichomonas vaginalis, and vaginal acidity with outcome of pregnancy. | bacterial vaginosis and trichomons vaginalis are believed to be the risk factors for preterm labor birth and preterm prelabor rupture of membranes. the objective of this study was to investigate the association between bacterial vaginosis, t.vaginalis, and vaginal ph with preterm labor birth and preterm prelabor rupture of membranes after excluding other known risk factors. | 2006 | 16859053 |
| [detection of mycoplasma hominis in trichomonas vaginalis by pcr]. | twelve of 28 isolates of trichomonas vaginalis collected from patients in hospitals in guangzhou were found naturally infected with mycoplasma hominis by pcr using specific primers. it suggests that the symbiosis of m. hominis in t. vaginalis also commonly occurs in china. | 2006 | 16862916 |
| [preparation, characterization and preliminary application of recombinant protein ap33 of trichomonas vaginalis]. | to clone ap33 gene of trichomonas vaginalis( t. v), construct prokaryotic expression system of the gene and identify its antigenicity and immunogenicity. | 2006 | 16866139 |
| [construction of eukaryotic expression recombinant plasmid of trichomonas vaginalis ferredoxin gene]. | total dna was extracted from t. vaginalis with chelex-100 method and used as templates for pcr. the ferredoxin gene was directionally cloned into plasmid pmd-18t vector and subcloned into eukaryotic expression vector pcdna3. 1 (+). the transformants were screened and identified by pcr and restriction analysis. the size of amplified ferredoxin gene was 306bp and the dna sequence of cloned gene was same with that published. | 2006 | 16866153 |
| predicting antitrichomonal activity: a computational screening using atom-based bilinear indices and experimental proofs. | existing trichomonas vaginalis therapies are out of reach for most trichomoniasis people in developing countries and, where available, they are limited by their toxicity (mainly in pregnant women) and their cost. new antitrichomonal agents are needed to combat emerging metronidazole-resistant trichomoniasis and reduce the side effects associated with currently available drugs. toward this end, atom-based bilinear indices, a new tomocomd-cardd molecular descriptor, and linear discriminant analysi ... | 2006 | 16875830 |
| lack of effectiveness of syndromic management in targeting vaginal infections in pregnancy in entebbe, uganda. | to measure the prevalence of reproductive tract infections (rtis) during pregnancy in entebbe, uganda, and to evaluate the current syndromic diagnosis and management approach in effectively targeting infections, such as bacterial vaginosis (bv) and trichomoniasis, that are associated with low birth weight and prematurity among newborns. | 2006 | 16877576 |
| trichomonas vaginalis initiator binding protein (ibp39) and rna polymerase ii large subunit carboxy terminal domain interaction. | the core promoter that directs rna polymerase to the start of transcription in the protist trichomonas vaginalis is an initiator (inr) element recognized by the inr binding protein, ibp39. this nuclear protein is composed of two domains: a 14.5 kda amino (n-terminal) and a 25 kda carboxy terminal domain (c-domain). here we describe the identification of an ibp39-interacting protein by screening a t. vaginalis expression library using a two-hybrid system with the ibp39 c-domain as bait. the ctd o ... | 2006 | 16879883 |
| genetic structure and evolution of the vps25 family, a yeast escrt-ii component. | vps25p is the product of yeast gene vps25 and is found in an endosomal sorting complex required for transport (escrt)-ii, along with vps22p and vps36p. this complex is essential for sorting of ubiquitinated biosynthetic and endosomal cargoes into endosomes. | 2006 | 16889659 |
| location of the cell-binding domain of cp65, a 65kda cysteine proteinase involved in trichomonas vaginalis cytotoxicity. | the cysteine proteinase (cp) of 65kda, cp65, binds to the surface of hela cells and is involved in trichomonas vaginalis cellular damage. to identify and locate the cp65 cellular-binding domain, we enriched the cp65 protein band by ammonium sulfate fractionation and ion-exchange chromatography and the n-terminal sequence was obtained. a 618bp gene fragment was obtained by pcr using genomic dna as template and primers derived from the n-terminal sequence of cp65 and the asn papain-catalytic conse ... | 2006 | 16891146 |
| prevalence of sexually transmitted infections among adolescents in kampala, uganda, and theoretical models for improving syndromic management. | to estimate the prevalence of treatable sexually transmitted infections (sti) in adolescents visiting a youth health clinic. to evaluate the algorithm for management of the abnormal vaginal discharge (avd) syndrome recommended in uganda's national guidelines and compare it with other theoretical flowchart models. | 2006 | 16488818 |
| throwing the dice for the diagnosis of vaginal complaints? | vaginitis is among the most common conditions women are seeking medical care for. although these infections can easily be treated, the relapse rate is high. this may be due to inadequate use of the diagnostic potential. | 2006 | 16503990 |
| the cost-effectiveness of treating male trichomoniasis to avert hiv transmission in men seeking sexually transmitted disease care in malawi. | allocation of funds to program areas where they may have an impact is critical to the success of any hiv control program. we examined the cost-effectiveness of providing first-line treatment for male trichomoniasis in malawi, a condition not commonly considered in syndromic management throughout sub-saharan africa. | 2006 | 16951650 |
| preterm birth due to maternal infection: causative pathogens and modes of prevention. | preterm birth represents a major problem for modern obstetrics due to its increasing frequency and the accompanying socioeconomic impact. although several maternal characteristics related to preterm birth have been identified, the etiology in most cases remains inadequately understood. various microorganisms have been linked to the pathogenesis of preterm birth. microbes may reach the amniotic cavity and fetus by ascending from the vagina and cervix, by hematogenous distribution through the plac ... | 2006 | 16953371 |
| viability of trichomonas vaginalis in urine: epidemiologic and clinical implications. | the impact of the viability of trichomonas vaginalis in urine on wet mount, culture, and pcr methods was assessed. to minimize the chance of false-negative results, urine specimens should be processed within 30 min of specimen collection and maintained at 37 degrees c, since temperature appears to affect the viability of trichomonas. | 2006 | 16928970 |
| [vaginal microbiocenosis in trichomoniasis]. | taxonomic complex of vaginal microflora and its persistence characteristics in trichomoniasis and microecological disturbances were studied. diminution of biotope colonization resistance was characterized by decreased autochthonous microorganisms' number and increased opportunistic microflora with expressed persistence. that could be of a great pathogenetic importance in the occurrence of reproductive tract inflammatory diseases of the bacterial etiology in addition of trichomoniasis in females. | 2006 | 16941883 |
| realtime pcr on urine sediments in the diagnosis of trichomoniasis in male subjects. | 2006 | 16942660 | |
| comparison between the gen-probe transcription-mediated amplification trichomonas vaginalis research assay and real-time pcr for trichomonas vaginalis detection using a roche lightcycler instrument with female self-obtained vaginal swab samples and male urine samples. | this study compared two assays for trichomonas vaginalis detection, gen-probe's transcription-mediated amplification (tma) assay for trichomonas vaginalis and btub fret pcr, using self-obtained clinical samples from 611 patients. infection status was defined as two positive results by two different tests. the initial tma assay sensitivity was 96.7%; specificity was 97.5%. the tma assay was comparable to btub fret pcr. | 2006 | 16943353 |
| persistent increase in the incidence of acute male urethritis diagnosed in general practices in france. | at-risk sexual behaviour seems to have increased in europe, possibly due to the reassuring efficacy of highly active antiretroviral treatments. | 2006 | 16464324 |
| the spermicidal and antitrichomonas activities of ssri antidepressants. | the study investigated spermicidal and antitrichomonas activities of selective serotonin reuptake inhibitor (ssri) antidepressants with a view to generate new lead for development of dual-function spermicidal microbicides, which is an urgent global need. fluoxetine, sertraline, and fluvoxamine exhibited both spermicidal and anti-sti (antitrichomonas) activities in vitro, whereas paroxetine and citalopram showed only the spermicidal activity. fluoxetine exhibited better activity profile than the ... | 2006 | 16464584 |
| multifarious transcriptional regulation of adhesion protein gene ap65-1 by a novel myb1 protein in the protozoan parasite trichomonas vaginalis. | the transcription efficiency of an adhesion protein gene, ap65-1, in trichomonas vaginalis varies with changes in the iron supply and with the growth stage. in the present study, two myb recognition elements, mre-1/mre-2r and mre-2f, were found to play antagonistic roles in regulating the iron-inducible activity of an ap65-1 reporter gene. intriguingly, either of these elements was shown to be sufficient to repress basal activity, but together they were also shown to activate growth-related acti ... | 2006 | 16467479 |
| decline in sexually transmitted infection prevalence and hiv incidence in female barworkers attending prevention and care services in mbeya region, tanzania. | to assess trends in sexually transmitted infection (sti) prevalence and hiv incidence and associated factors in a cohort of female barworkers exposed to behavioural interventions and sti screening and treatment. | 2006 | 16470126 |
| results of longterm hospital based cytological screening in asymptomatic women. | routine cytological screening has been carried out in 27,062 asymptomatic women attending gynaec and family planning o.p.d. of queen mary's hospital, lucknow, india (april 1971-december 2004). incidence of squamous intraepithelial lesion (sil) was found to be 5.9% in the series, while cervical malignancy was seen in 0.6% of cases. the study highlighted the immense utility of cytological screening in minimizing the incidence of carcinoma cervix in the segment of the urban population screened, as ... | 2006 | 16470854 |
| trichomonas vaginalis promotes apoptosis of human neutrophils by activating caspase-3 and reducing mcl-1 expression. | neutrophils are the predominant inflammatory cells found in the vaginal discharge of patients with trichomonas vaginalis infection. however, it is not known whether neutrophil apoptosis is induced by live t. vaginalis. therefore, we examined whether t. vaginalis can influence neutrophil apoptosis, and also whether caspase-3 and the bcl-2 family members are involved in the apoptosis. thus, human neutrophils were incubated with live t. vaginalis and neutrophil apoptosis was evaluated by giemsa, an ... | 2006 | 16916367 |
| [the incidence of trichomonas vaginalis in vaginal specimens from gynecologic patients]. | the aim of this preliminary study was to determine the incidence trichomonas vaginalis in our area. the group under investigation consisted of gynecologic patients with various complaints who came to the gynecology polyclinic of the cumhuriyet university medical hospital. vaginal smears were taken from the posterior fornics of these patients. three samples were taken from each person with sterile swaps. one of the samples was taken for cultivation, one for a direct preparation and the last for g ... | 2006 | 17160845 |
| the combined effect of two aqueous extracts on the growth of trichomonas vaginalis, in vitro. | in this study a trial was performed to determine the effect of a combination of two aqueous extracts of two medicinal plants namely the violet, viola odorata, and the rue, ruta graveolens, with concentrations of 0.15625, 0.3125, 10-20 mg/cm3 on the growth of trichomonas vaginalis cultured in (cm161) medium during periods of 24, 48, 72, and 96 hrs. the results showed that there is a variation of inhibition. complete inhibition was seen with a concentration 10 mg/cm3 for 48 hrs. during the 96 hr. ... | 2006 | 17309025 |
| [causation and treatment of infectious leucorrhoea at the cocody university hospital (abidjan, côte d'ivoire)]. | to determine the frequency of different bacteria causing infectious leucorrhoea and to calculate the percentage of cures after standard treatment. | 2006 | 17284396 |
| [spontaneous urogenital infection in macaques]. | rates of natural infection of macaques with microorganisms that cause urogenital tract infections (ugt)--chlamydias, mycoplasms, ureaplasms and trichomonades--have been assessed. 198 macaques (rhesus, javan and lapunder) aged from newborn to 25 years (born healthy or from females with complicated pregnancy or labour) were examined. high rate of infection (40%) was observed in healthy macaques by pcr assay. in animals born from females with complicated pregnancy or labour (abortion, complicated l ... | 2006 | 17297890 |
| trichomonas vaginalis infection. | 2006 | 17151049 | |
| comparison between secretory leukocytic protease inhibitor and reactive nitrogen intermediates levels in cervicovaginal secretions from symptomatic and asymptomatic trichomoniasis egyptians patients. | although trichomoniasis is one of the most widespread sexually transmitted diseases, limited information is known about the host and parasite factors which cause symptomatic versus asymptomatic infections. both of secretory leukocytic protease inhibitor (slpi) and reactive nitrogen intermediates (rni) are major effectors in the innate immune response against infection. this study aimed to compare the level of slpi and rni in relation to the vaginal complains among trichomoniasis egyptian patient ... | 2006 | 17153694 |
| trichomoniasis among women with vaginal discharge in benghazi city, libya. | from april 2000 to july 2001, clinical and wet-mount examination were performed on 2,450 women attending gynecologic department, el-keish polyclinic, benghazi city. the mean age was 34+/-7 (in years). 328 (13.4%) women were clinically diagnosed as having viginatis. sterile vaginal swab of the posterior fornix was stained by giemsa and examined as wet smear for trichomoniasis infection. 29/2450, (1.2%) had t. vaginalis. the infected women suffered vaginalis discharge (93.10%), burning (81.48%), v ... | 2006 | 17153709 |
| proteins of the glycine decarboxylase complex in the hydrogenosome of trichomonas vaginalis. | trichomonas vaginalis is a unicellular eukaryote that lacks mitochondria and contains a specialized organelle, the hydrogenosome, involved in carbohydrate metabolism and iron-sulfur cluster assembly. we report the identification of two glycine cleavage h proteins and a dihydrolipoamide dehydrogenase (l protein) of the glycine decarboxylase complex in t. vaginalis with predicted n-terminal hydrogenosomal presequences. immunofluorescence analyses reveal that both h and l proteins are localized in ... | 2006 | 17158739 |
| determination of cervicovaginal microorganisms in women with abnormal cervical cytology: the role of ureaplasma urealyticum. | to evaluate the existence of an association between cervicovaginal infections and precancerous lesions of the uterine cervix, through determination of prevalent cervicovaginal micro-organisms, alone and in association with human papillomavirus (hpv), in patients with abnormal and normal vaginal cytology. | 2006 | 17214350 |
| alternative drugs against trichomonas vaginalis. | to investigate the effect of drugs other than metronidazole, 3 non-pregnant women infected with trichomonas vaginalis were treated with doxycycline, 2 x 200 mg/day for 1 week. another 3 women were treated with praziquantel, single dose, 40 mg/kg body weight. no therapeutic effect was detected for either drug. in vitro, oxytetracycline led to death of t. vaginalis at a concentration of 15 mg in 0.5 ml medium. extract of myrtus communis caused death of t. vaginalis at ph 4.65, but failed to do so ... | 2006 | 17333809 |
| [study on genetic approach in the detection of cryptosporidium parvum and giardia lamblia in acquired immunodeficiency syndrome patients]. | to establish genetic method in detecting cryptosporidium parvum and giardia lamblia which often coinfected with aids patients. | 2006 | 17343184 |
| ureaplasma urealyticum and mycoplasma hominis infection in women with urogenital diseases. | the aim of the study was to assess the incidence of ureaplasma urealyticum (u. urealyticum) and mycoplasma hominis (m. hominis) infection in women with urogenital diseases. | 2006 | 17357319 |
| [molecular and chemical mechanism of trichomonas vaginalis-induced contact-dependent cytotoxicity]. | trichomonas vaginalis parasitizes in human genitourinary tract. the protozoon adhering to target cell plays a critical role in its contact-dependent cytotoxicity. the enzymes synthesized by t.voginalis can hurt vaginalis epithelial cells (vecs) directly. the focal immune reaction in the location parasitized by the parasite may provide an immunologic protection. meanwhile, inflammatory factors and immune cells may aggravate the situation. in general, the t. vaginalis-induced contact-dependent cyt ... | 2006 | 17361821 |
| [cloning and sequence analysis of a partial gene of trichomonas vaginalis dsrna virus]. | a pair of degenerate primers were designed following the published nucleotide sequence of trichomonas vaginalis virus(u08999, nc003873, nc003824, nc003834). using the total nucleic acids extracted from the trichomonas vaginalis as template, rt-pcr was performed with the primers to obtain a fragment of the tvv. the product was cloned, sequenced and compared with the sequences available in the genbank. the size of the amplified gene was 1 454bp, which shares 82.9% sequence identity with the tricho ... | 2006 | 17361826 |