Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [antibiotic resistance of anaerobic bacteria (author's transl)]. | reference data on the overall resistance patterns of anaerobic bacteria are needed since in individual clinical cases susceptibility testing is complicated by the long generation time and difficulties of test conditions. benzylpenicillin and tetracyclines are the drugs of choice against clostridium perfringens. newer cephalosporins are also active, whereas other clostridium species have been found resistant. beta-lactam antibiotics show high activity against peptococcaceae; resistance to clindam ... | 1982 | 6286503 |
| partial purification and properties of phosphatidylserine synthase from clostridium perfringens. | the membrane-associated phospholipid biosynthetic enzyme cytidine 5'-diphospho-1,2-diacyl-sn-glycerol:l-serine o-phosphatidyltransferase (phosphatidylserine synthase; ec 2.7.8.8) was partially purified 337-fold from a cell-free extract of the gram-positive pathogenic anaerobe clostridium perfringens (atcc 3624). the purification procedure included extraction from the cell envelope with the nonionic detergent triton x-100, followed by affinity chromatography on cytidine 5'-diphosphate-diacylglyce ... | 1982 | 6286597 |
| rapid and sensitive sialidase assay by high-performance liquid chromatography and its application to detection of sialidase in human urine. | 1982 | 6286699 | |
| a simple device for growing clostridium perfringens and its application in bacteriocin studies. | a simple device constructed of common laboratory material served as a minifermenter for the growth of clostridium perfringens. a constant flow of nitrogen gas into a culture tube containing c. perfringens assured agitation of the culture and a mechanism for dispensing small volumes of liquid from the culture without disturbing the growth environment. the method was applied to examining the growth-inhibiting effect of bacteriocins of c. perfringens where a very economical use of radioactive isoto ... | 1982 | 6288212 |
| quantitation of binding and subcellular distribution of clostridium perfringens enterotoxin in rat liver cells. | binding of enterotoxin from clostridium perfringens type a was studied in suspensions of parenchymal and nonparenchymal cells from rat liver. in hepatocytes, 1.5 x 10(6) specific binding sites per cell with an association constant of 3.2 x 10(6) m-1 were found. about 1% of the added toxin was nonspecifically bound to the hepatocytes. at concentrations of toxin below 0.1 micrograms/ml, 80% of the toxin density of 7 x 10(6) cells per ml. binding did not increase after the cells became permeable to ... | 1982 | 6288564 |
| purification of chlamydia trachomatis lymphogranuloma venereum elementary bodies and their interaction with hela cells. | a procedure has been developed to yield infectious elementary bodies of the lymphogranuloma venereum strains lgv 434 and 404 of chlamydia trachomatis, labelled during intracellular growth in hela 229 cells. the final preparation, obtained after velocity sedimentation of a polycarbonate membrane-filtered sample through a sucrose gradient, is free of host proteins and, more importantly, of chlamydial reticulate bodies. using such purified preparations, it was found that the association of lgv 434 ... | 1982 | 6288839 |
| implication of plasma free hemoglobin in massive clostridial hemolysis. | prolonged tissue oxygenation and maintenance of intravascular oncotic pressure are severely impaired with the absence of an effective rbc mass. we recently encountered a patient with a nondetectable hematocrit value (0%) attributed to clostridium perfringens hemolysis. the patient maintained normal bp, tissue oxygenation, and mentation and survived longer than four hours, despite ineffective transfusion therapy. plasma free hemoglobin was responsible for the preservation of tissue oxygenation, i ... | 1982 | 6288977 |
| [clinicomorphological characteristics of suppurative wounds treated with iruksol]. | 1982 | 6289004 | |
| methane and hydrogen production by human intestinal anaerobic bacteria. | the gas above liquid cultures of a variety of human intestinal anaerobic bacteria was sampled and analysed by headspace gas chromatography. hydrogen production was greatest with strains of the genus clostridium, intermediate with anaerobic cocci and least with bacteroides sp. very few strains produced methane although small amounts were detected with one strain of b. thetaiotaomicron, c. perfringens and c. histolyticum. there may be a relationship between these anaerobic bacteria and several gas ... | 1982 | 6289602 |
| [method of determining the antibiotic sensitivity of anaerobic microorganisms using standard disks]. | three variants of the procedure for determination of antibiotic sensitivity in anaerobic microorganisms with the use of standard paper discs were developed. according to the first variant the solid nutrient medium is melted at 46 degrees c and mixed with the culture of the microbe being tested. the mixture is added to the cover of a petri dish. when the medium becomes solid, antibiotic sensitivity discs are placed onto the agar surface. after that one more layer of the medium is added. the mediu ... | 1982 | 6289725 |
| examination of bovine faeces for the isolation and identification of clostridium species. | 1982 | 6279555 | |
| [growth and fatty acid composition of clostridium perfringens cells under constant ph values]. | 1982 | 6278011 | |
| acute gastric dilatation in nonhuman primates: review and case studies. | acute gastric dilatation occurs sporadically in laboratory-housed nonhuman primates. clinical histories often include chronic drug administration, food restriction, accidental overfeeding, and prior anesthesia. monkeys may be found dead or may have clinical signs of colic, abdominal distention, and dyspnea. death in untreated cases is due to impaired venous return and cardiopulmonary failure. gastric distention with fermented gaseous ingesta and congestion of the abdominal viscera are the predom ... | 1982 | 6153002 |
| change in conformation of various dnas on melting as followed by circular dichroism. | 1982 | 6277406 | |
| stimulation of sporulation of clostridium perfringens by papaverine. | papaverine induced sporulation in clostridium perfringens, strains fd-1 and ps52; growth was markedly slowed under these conditions. papaverine induced sporulation in the presence of glucose, a sporulation repressor, although increasing glucose concentrations overcame the papaverine effect. papaverine induced sporulation of strain fd-1 more effectively than did theophylline. | 1982 | 6277684 |
| cdp-diacylglycerol synthase activity in clostridium perfringens. | ctp:phosphatidate cytidylyltransferase (cdp-diacylglycerol synthase; ec 2.7.7.41) was identified in the cell envelope fraction of the gram-positive anaerobe clostridium perfringens. the association of this enzyme with the cell envelope fraction of cell extracts was demonstrated by glycerol density gradient centrifugation and by activity sedimenting with the 100,000 x g pellet. the enzyme exhibited a broad ph optimum between ph 6.5 and ph 7.5. enzyme activity was dependent on magnesium (5 mm) or ... | 1982 | 6275792 |
| microbiological studies on hamburgers. | one hundred and eighty-two raw, 112 pre-cooked and 750 cooked hamburgers composed mainly of beef or beef and pork were subjected to microbiological examination.raw hamburgers gave total bacterial counts from 10(6) to 10(8) per g, counts of enterobacteriaceae from 10(4) to 10(6) per g, of escherichia coli from 10(3) to 10(5), of group d streptococci from 10(2) to 10(4), of staphylococcus aureus from 3 to 10(2) and of clostridium perfringens less than 10 bacteria per g. of the samples, 32% contain ... | 1982 | 6276464 |
| [dry medium for isolating clostridium perfringens]. | the possibility of using blood substitutes (hydrolysin, casein hydrolysate "tsolipk" and amino peptide) with expired shelf life as a culture medium for the isolation of cl. perfringens has been studied. dry culture medium based on these inedible products has been developed. to stimulate bacterial growth, fodder yeast extract has been used. the suppression of the growth of extraneous microflora is ensured by the use of antibiotics: polymyxin sulfate and mycerin sulfate. the recommended medium is ... | 1982 | 6282016 |
| human neutrophil peptide receptors: mobilization mediated by phospholipase c. | incubation of human neutrophils with phospholipase c from clostridium perfringens caused an increase in the ability of the treated cells to bind the chemotactic peptide, f-met-leu-phe. the increase in binding was related to an increase in specific binding of the ligand. the increase in specific binding was, in turn, related to an increased number of peptide receptors. the dissociation constant (kd) for the tripeptide was not altered, on the average, by enzyme treatment. the increase in peptide r ... | 1982 | 6282127 |
| effects of cholesterol evulsion on susceptibility to perfringolysin o of human erythrocytes. | human erythrocytes preincubated with a phosphatidylcholine suspension (preincubated cells) showed decreased susceptibility to perfringolysin o, the decrease being strongly affected by preincubation time and temperature, and the phosphatidyl choline concentration. the binding of the toxin to the preincubated cells also decreased with the preincubation time and reached minimum at 37 degrees c for 6 h. through this preincubation, about 30% of cholesterol was removed from cells without lysis. the su ... | 1982 | 6282326 |
| interactions of dna with divalent metal ions. iv. competitive studies of mn2+ binding to at- and gc-rich dnas. | 1982 | 6282356 | |
| [clostridium perfringens sepsis following criminal abortion]. | 1982 | 6281674 | |
| in vivo effects of enterotoxin from clostridium perfringens type a in the rabbit colon: binding vs. biologic activity. | rabbit colonic loops were exposed to purified enterotoxin from clostridium perfringens type a and perfused for analysis of the effects of the enterotoxin on transport and surface structure of the colon. the colon showed no apparent response to the enterotoxin in the transport of fluid and electrolytes as compared with untreated animals. there was a significant increase in levels of luminal fluid protein, corresponding to an observed increase in secretion of mucus. histologic sections showed no e ... | 1982 | 6279735 |
| radiolytic pathways in gamma-irradiated dna: influence of chemical and conformational factors. | 1982 | 6278529 | |
| clostridium perfringens empyema unresponsive to penicillin. | 1982 | 6279898 | |
| [effect of passage on the fatty acid composition of the lipids in clostridium perfringens a]. | 1982 | 6280021 | |
| increase of plasma neuraminidase activity in experimental peritonitis. | 1982 | 6280198 | |
| immune regulatory factors in sheep fetuses. | after an antigenic stimulus in vitro, a fraction was found in the supernatant fluids of spleen (s-1) and thymus (t-1) cell cultures of 70 to 80-day-old sheep fetuses infected with ovine adenovirus in utero. the fraction agreed with alpha-1-fetoprotein in mobility and its molecular weight was 67 000. injected into adult sheep, s-1 and t-1 had a suppressive effect on the humoral immune response to both adenovirus and clostridium perfringens d vaccine. tests with peripheral lymphocytes suggested th ... | 1982 | 6283803 |
| a preliminary study on the pathogenicity of axenically grown entamoeba histolytica for the golden hamster. | 1982 | 6284104 | |
| significance of clostridial bacteremia. | 1982 | 6280507 | |
| microbiology of ova harvested from commercially slaughtered mature chickens. | the microbiological quality of ova harvested from mature chickens in commercial slaughter plants was studied. greatest contamination of the product occurred at the location where ova were drawn from the carcasses. water fluming and chilling procedures caused some reduction in contamination levels. total aerobic plate counts, coagulase-positive staphylococci, presumptive clostridium perfringens and enterobacteriaceae counts of chilled ova were low, and no salmonellae were detected on these sample ... | 1982 | 6283510 |
| [emphysematous cholecystitis. report of 2 cases]. | 1982 | 6283608 | |
| tampon associated toxic shock syndrome. | 1982 | 6283629 | |
| the potential of bacteriocin typing in the study of clostridium perfringens food poisoning. | a range of 49 bacteriocins was used to type 311 strains of clostridium perfringens isolated from food poisoning outbreaks. strains of same serotype within an outbreak showed similar patterns of susceptibility to bacteriocins, whereas strains of different serotype isolated from different sources produced many variations in bacteriocin susceptibility patterns. the 311 strains, along with isolates from a wide range of sources were screened for their ability to produce bacteriocins. a much greater p ... | 1982 | 6294146 |
| isolation and properties of metronidazole-resistant mutants of clostridium perfringens. | clostridium perfringens strains resistant to metronidazole and tinidazole were isolated from the sensitive parent strain cm288 after mutagenesis with n-methyl-n'-nitro-n-nitrosoguanidine. strain cm288 was already resistant to rifampicin and nalidixic acid; these genetic markers helped to confirm the identity of mutants. all mutants showed similar characteristics: they grew more slowly than the parent strain and failed to reach the same maximum turbidity; uptake of metronidazole and tinidazole fr ... | 1982 | 6294298 |
| [neonatal cecal perforation. clostridium perfringens enterocolitis with septicemia and hemolysis. total megacolon]. | 1982 | 6294584 | |
| [necrotic enteritis in piglets due to clostridium perfringens type "c"]. | 1982 | 6294823 | |
| [isolation of a purified theta-hemolysin from c1. perfingens type a and its use for titrating toxins and sera]. | purified cl. perfringens theta-hemolysin with a specific activity of 15-16 x 10(5) hu per mg of protein nitrogen, toxic for mice when injected intravenously, has been obtained by precipitation with zinc chloride, gel filtration through sephadex g-100 and ion-exchange chromatography on deae sephadex a-50. 1 dcl of the toxin is 0.2-0.4 mg. the yield of theta-hemolysin amounting to 20-25% has been achieved, the degree of its purification reaching 80- to 120-fold. equine anti-theta hemolytic serum h ... | 1982 | 6295033 |
| clostridium perfringens type a toxins in relation to nephritis and uric acid diathesis in captive willow ptarmigan (lagopus l. lagopus). | 1982 | 6295118 | |
| extraction of clostridium perfringens spores from bottom sediment samples. | two extraction-separation procedures were developed and evaluated for use in conjunction with the mcp membrane filter method for the enumeration of clostridium perfringens spores in bottom sediments. in the more facile of the two procedures, a distilled-water suspension of the sediment sample is pulse sonicated for 10 s and allowed to settle. portions of the supernatant are then removed for membrane filtration. this procedure is recommended for general use. the more complicated procedure is reco ... | 1982 | 6295278 |
| selective cytotoxicity of clostridium perfringens delta toxin on rabbit leukocytes. | clostridium perfringens delta toxin was selectively cytotoxic for various rabbit leukocyte populations. the sensitivity of these populations to the toxin varied, depending on the tissue from which they were derived, from 28% (appendix) to 41% (bone marrow) and 32% (spleen). macrophages were uniformly killed by delta toxin, whereas thymocytes were essentially resistant. selective cytotoxicity was correlated to the specific binding of the radiolabeled toxin by target cells. the relationship betwee ... | 1982 | 6295950 |
| in-vitro activity of ceftizoxime against anaerobic bacteria and comparison with other cephalosporins. | 1982 | 6296028 | |
| percutaneous fixation of left displaced abomasum, using a bar suture. | 1982 | 6284690 | |
| a comparison of the levels of faecal indicator bacteria in water and human faeces in a rural area of a tropical developing country (sierra leone). | the levels of faecal coliforms (fc), indole-positive fc (presumptive escherichia coli), faecal streptococci (fs), streptococcus faecalis and clostridium perfringens in the natural water sources used by 29 rural settlements in sierra leone were investigated. levels of the same indicators in human faeces were also investigated. the incidence of salmonella spp. in both habitats and the temperature, ph and conductivity of water sources were also recorded. all water sources were contaminated with the ... | 1982 | 6284838 |
| clinical laboratory evaluation of a reverse camp test for presumptive identification of clostridium perfringens. | ninety-six percent of clostridium perfringens isolates from clinical specimens were reverse camp test positive, whereas several other clostridium species tested were reverse camp test negative. c. perfringens was detected by direct inoculation of clinical specimens to reverse camp plates, and the reverse camp procedure provided reliable presumptive identification of this organism. | 1982 | 6296196 |
| [method of obtaining an erythrocyte diagnosticum for detecting cl. perfringens type a enterotoxin]. | erythrocytic immunoglobulin diagnostic reagent for detecting cl. perfringens enterotoxin in the indirect hemagglutination test has been obtained. the diagnostic reagent has been prepared on the basis of antibodies of cl. perfringens enterotoxin with the use of acroleinized tanned sheep red blood cells. the indirect hemagglutination test with this reagent is characterized by high specificity and sensitivity (the method permits to detect enterotoxin protein at a concentration of up to 0.005 microg ... | 1982 | 6297189 |
| [protective properties of theta-hemolysin obtained by affinity chromatography]. | the data on the study of the protective activity of theta hemolysin and cl. perfringens lecithinase preparations and the corresponding antitoxic sera obtained by indirect immune affinity chromatography are presented. experiments in mice and guinea pigs indicate that the injection of antihemolytic serum and immunization with anatheta hemolysin ensures the protection of the animals from theta toxin. the enrichment of analecithinase preparation with anatheta hemolysin has been found to increase its ... | 1982 | 6297191 |
| clostridium perfringens meningitis. | in this report, primary clostridium perfringens meningitis, a rare condition, is described in a 62-year-old man who developed fatal clostridial meningitis following surgical evacuation of a subdural hematoma. this is the third case report of clostridial meningitis as a complication of craniotomy. clostridial infection should be considered in any patient with meningitis with a history of surgical intervention, or head injury, even if only slight. | 1982 | 6298137 |
| mechanism of antimicrobial resistance and resistance transfer in anaerobic bacteria. | the antimicrobial susceptibility pattern of anaerobic bacteria has been changing over the past decade. this paper reviews the mechanisms by which these organisms have become resistant to selected antibiotics and reviews data demonstrating that bacteroides fragilis and clostridium perfringens possess systems for transferring resistance determinants. within bacteroides there is widespread resistance to penicillins, cephalosporins and tetracycline compounds while there have been reports of resistan ... | 1982 | 6300995 |
| [clostridium perfringens as a cause of food poisoning]. | 1982 | 6301099 | |
| [emphysematous cholecystitis. microbiological study of a case]. | 1982 | 6298911 | |
| [antibacterial effects of niridazole. ii. effects on aerobic and anaerobic bacteria]. | niridazole which is chemically related to metronidazole is endowed with much better antibacterial activity. first, several genera of aerobic bacteria, such as salmonella and escherichia, are susceptible to niridazole, whereas metronidazole is completely ineffective. there exist, however, some particular strains which are naturally resistant to niridazole. the in vitro activity of niridazole is still increased, if these facultative anaerobic bacteria are tested under anaerobic growth conditions. ... | 1982 | 6299027 |
| enteritis necroticans (pigbel) an unrecognised preventable disease? | 1982 | 6299659 | |
| detection of clostridium perfringens type a enterotoxin by elisa. | 1982 | 6301213 | |
| further development of a bacteriocin typing system for clostridium perfringens. | 1982 | 6302063 | |
| [in vitro susceptibility of clostridium perfringens to 6 antimicrobial agents]. | 1982 | 6306749 | |
| antibody response to pigbel vaccine in infants less than one year old. | 1982 | 6306949 | |
| virginiamycin effects on controlling necrotic enteritis infection in chickens. | duplicate trials were conducted with male broiler chickens to evaluate virginiamycin as treatment against experimentally induced necrotic enteritis infection. each trial consisted of seven treatments, each replicated four times, with 10 birds per replicate. two treatments were fed control ration (noninfected control and infected control) and the five remaining treatments were fed virginiamycin at 5, 10, 15, 20, or 40 g/ton. birds were orally dosed with 10 ml of clostridium perfringens culture at ... | 1982 | 6806795 |
| the bacterial flora of neonates with congenital abnormalities of the gastro-intestinal tract. | the development of the bacterial flora of neonates with congenital abnormalities of the gastro-intestinal tract was studied in 31 infants during the first 10 days of life. specimens were collected from the umbilicus, mouth and gastro-intestinal tract on the pre-operative day, at operation and on post-operative days 1, 2, 3, 5, 7 and 10. bacteria were isolated semi-quantitatively on a variety of plain and selective media and identified by conventional methods.staphylococcus albus was the predomin ... | 1982 | 7057028 |
| fatal clostridium perfringens infection after normal term pregnancy. | we describe a case of fatal gas gangrene of the uterus after an apparently normal term pregnancy in a 22-year-old woman. the patient died less than 15 h after the onset of symptoms, which consisted of a single episode of vomiting and increasing hypogastric pain. hypotension, tachycardia and renal failure were the dominant clinical features. the pathogenesis, diagnosis and treatment of uterine gas gangrene are discussed. | 1982 | 7073337 |
| [necrotising enteritis (author's transl)]. | 4 cases of severe necrotising enteritis are dealt with. the small intestine was affected 3 times, the colon once, one patient died after resection of the affected bowel segment. in 2 additional cases a large resection of the small intestine became necessary. one patient was treated conservatively. the bacteriological evidence of clostridium perfringens type c in the intestinal wall allows the assumption of a primarily bacterial genesis of this disease. its aetiology, clinical and pathological-an ... | 1982 | 7102142 |
| [acute clostridium perfringens peritonitis in prolonged presence of a pessary]. | 1982 | 7106632 | |
| malignant oedema caused by clostridium perfringens type a in a horse. | 1982 | 7120271 | |
| pathogenic anaerobes. | anaerobes are prevalent on all mucosal surfaces and virtually all anaerobic infections are endogenous. two thirds of anaerobic infections involve five anaerobic organisms or groups--the bacteroides fragilis group, the bacteroides melaninogenicus-bacteroides asaccharolyticus group, fusobacterium nucleatum, the anaerobic cocci, and clostridium perfringens. conditions that lower the oxidation-reduction potential and disrupt the mucosal surface (eg, vascular problems, malignant neoplasms, and surger ... | 1982 | 7125789 |
| water disinfection with the hydrogen peroxide-ascorbic acid-copper (ii) system. | treatment of secondary effluents with hydrogen peroxide (10 mg/liter)-ascorbic acid (10 mg/liter)-cu2+ (0.5 mg/liter) for 60 min resulted in around 99% reduction of the initial plate count. hydrogen peroxide could be replaced by other peroxygen compounds; ascorbic acid could be replaced by other reducing agents, of which sodium sulfite and ethanol were the most effective. cu2+, however, could not be replaced by other metal ions without loss of bactericidal efficiency of the ternary combination. ... | 1982 | 7138000 |
| [binding of cationized ferritin to the surface of the palatine crest in the rat]. | recently, we suggested that the cell coat of the surface epithelial cells of the palatal shelves and especially its sialyl groups are implied in the adhesion process which initiates the constitution of the secondary palate. in this work, use of cationized ferritin which interacts with anionic receptors of the plasma membrane give us the possibility to render these sialic acids visible. after five minutes incubation in presence of cationized ferritin, the labeling particles are distributed in mon ... | 1982 | 7139138 |
| [gas gangrene caused by clostridium perfringens infection]. | 1982 | 7154794 | |
| biotransformation of 1-nitropyrene in intestinal anaerobic bacteria. | mutagenic nitroaromatic compounds have recently been found in photocopies, urban atmosphere, automobile exhaust and wastewater. 1-nitropyrene (1-np) is readily formed when pyrene, ubiquitous in the environment, is exposed to nitrogen dioxide in the urban atmosphere or in automobile exhaust, and is highly mutagenic, inducing 449 his+ revertants/plate/nmol from salmonella typhimurium strain ta98 in the absence of s9 fraction in the salmonella-microsome test. it is possible to swallow sputum or som ... | 1982 | 7167066 |
| age-development changes in susceptibility of erythrocytes to perfringolysin o. | susceptibility to perfringolysin o of erythrocytes from mice of different ages was examined. erythrocytes of mice younger than 5 weeks' old were more resistant to the toxin than those of young adult and adult mice. erythrocytes of aged mice were about 3.5 times more susceptible to the toxin than erythrocytes from 4-week-old mice. the membrane cholesterol content of erythrocytes appeared to be maintained at a constant level throughout the ages of mice examined. about 5% of the total membrane chol ... | 1982 | 7176704 |
| [microbiological studies of beef tartar]. | two hundred lots of 'filet américain' (a mixture of minced meat, acid sauce, condiments, salt, etc., meant to be eaten raw) were sampled from retailers and examined for several types of micro-organism; 185 lots had been prepared by the retailer, fifteen on an 'industrial' scale. pork had been used in seventy-three lots (including fourteen 'industrial' lots); beef was present in all lots, horse meat in none of them. on the whole, the bacterial state of the meats in which pork had been used was fo ... | 1982 | 7179231 |
| n-formimidoyl thienamycin (mk0787): in vitro activity against anaerobic bacteria. | the in vitro activity of n-formimidoyl thienamycin (mk0787) was tested against 239 anaerobic bacteria clinical isolates: 70 of bacteroides fragilis, 18 of b. distasonis, 16 of b. thetaiotaomicron, 10 of b. vulgatus, 24 of bacteroides spp., 22 of b. melaninogenicus (all three subspecies), 26 of fusobacterium spp., 10 of peptococcus spp., 15 of peptostreptococcus spp., 15 of clostridium perfringens, and 13 of clostridium spp. ninety-five percent of the isolates were inhibited by less than or equal ... | 1982 | 6952818 |
| in vitro and in vivo activity of dl-8280, a new oxazine derivative. | dl-8280, 9-fluoro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-2,3-dihydro-7h- pyrido-(1,2,3-de)1,4-benzoxazine-6-carboxylic acid, is a new nalidixic acid analog with a broad spectrum of antibacterial activity against gram-negative and gram-positive bacteria, including obligate anaerobes. the activity of dl-8280 against enterobacteriaceae, pseudomonas aeruginosa, haemophilus influenzae, neisseria gonorrhoeae, and clostridium perfringens was roughly comparable to that of norfloxacin and far exceede ... | 1982 | 6960805 |
| [location of exchangeable cations within the spore of clostridium perfringens]. | 1982 | 6296480 | |
| enrichment medium and control system for isolation of campylobacter fetus subsp. jejuni from stools. | enrichment culture with a semisolid medium increased by 6% the isolation rate of campylobacter fetus subsp. jejuni. the semisolid enrichment medium was also used successfully as a transport medium for campylobacter isolates. a blood agar plate streaked with pseudomonas aeruginosa, clostridium perfringens, and a laboratory strain of campylobacter was a good control system for the microaerophilic atmosphere. good growth of all three organisms indicated satisfactory conditions for culturing campylo ... | 1982 | 6764764 |
| [production, purification and characterization of some clostridium perfringens toxins]. | cultures of clostridium perfringens produce a factor which hydrolyzes phospholipids. in this paper we report the separation and the purification from the growth medium of clostridium perfringens of two phospholipase activities: a specific sphingomyelinase, and a specific lecithinase. both the phospholipase activities have been purified 300-fold by fractionation of the growth medium with ammonium sulfate, filtration on sephadex g-100 and chromatography on deae cellulose. the sphingomyelinase acti ... | 1982 | 6315516 |
| benzylpenicillin-induced filament formation of clostridium perfringens. | growth of clostridium perfringens with low concentrations of benzylpenicillin inhibited septum formation and division of the organisms. this resulted in continued growth of the organisms as aseptate filaments. the effect was reversed on removal of the antibiotic. the composition of walls isolated from organisms grown with the antibiotic was similar to that of walls from untreated bacteria. in addition, both contained non-n-acetylated glucosamine residues in their peptidoglycan. no differences we ... | 1982 | 6306143 |
| hemorrhagic enterotoxemia caused by clostridium perfringens type c in a foal. | a four day old appaloosa foal in alberta died from hemorrhagic enterotoxemia. beta-toxin of clostridium perfringens was demonstrated in the intestinal contents of the foal and a pure culture of c. perfringens type c was grown from the small intestine. histological examination showed hemorrhage and extensive necrosis of the small intestinal mucosa. areas of necrotic tissue were surrounded by massive numbers of clostridia-like rods. there was also a moderate degree of thyroid hyperplasia. this is ... | 1982 | 17422190 |
| case report of necrotic enteritis in neonatal pigs caused by clostridium perfringens type c. | 1983 | 6306319 | |
| metabolism of primary bile acids by clostridium perfringens. | 1983 | 6306343 | |
| lincomycin dose response for treatment of necrotic enteritis in broilers. | a dose response study was conducted to determine the appropriate (optimal) lincomycin concentration in drinking water for the treatment of necrotic enteritis (ne) in broilers. the study was replicated twice over time using a total of 2,895 broilers. birds were raised in a facility containing a built-up litter from a source that experienced ne. they were commingled from 1 day of age until ne was observed. lincomycin was mixed in drinking water at a rate of 0, .528, 2.114, 8.454, or 33.818 mg/lite ... | 1983 | 6306631 |
| therapeutic effect of optimal lincomycin concentration in drinking water on necrotic enteritis in broilers. | the efficacy of 16.9 mg lincomycin/liter of drinking water was evaluated for the treatment of necrotic enteritis (ne) in 743 broiler-type chickens. birds were raised in a facility containing a built-up litter obtained from a source that experienced ne. they were commingled from 1 day of age until ne was observed. two groups of 6 pens each were given 0 or 16.9 mg lincomycin/liter of drinking water. water medication was offered fresh daily for 7 days and the study was terminated 3 weeks after init ... | 1983 | 6306632 |
| transferable tetracycline resistance in clostridium perfringens strains of porcine origin. | these studies represent the first systematic survey of the incidence of conjugative antibiotic resistance in clostridium perfringens. ninety-two antibiotic-resistant porcine strains were examined to see if they could donate their antibiotic-resistance determinants to sensitive recipient strains. fifteen of the 89 tetracycline-resistant strains transferred their tetracycline resistance in mixed-plate mating experiments but no transfer of macrolide-lincosamide resistance was detected. the efficien ... | 1983 | 6318939 |
| effect of clostridium perfringens epsilon toxin on the cardiovascular system of rats. | pressor activity was demonstrated by the intravenous injection of purified epsilon toxin of clostridium perfringens type d but not by that of epsilon prototoxin. the activity was completely abolished by c. perfringens type b or d antiserum, but not by type a, c, or e antiserum. the rise in blood pressure caused by the toxin was accompanied by a decrease in the blood flow in skin without any change of heart rate and electrocardiogram readings. these results indicate that epsilon toxin possesses p ... | 1983 | 6315593 |
| phorbol esters, phospholipase c, and growth factors rapidly stimulate the phosphorylation of a mr 80,000 protein in intact quiescent 3t3 cells. | addition of biologically active phorbol esters to intact quiescent 3t3 mouse cells stimulates an extremely rapid (detectable within seconds) phosphorylation of a mr 80,000 cellular protein (termed "80k"). phorbol 12,13-dibutyrate enhances 80k phosphorylation in a dose-dependent manner; half-maximal effect is obtained at 32 nm. the possibility that this phosphorylation is related to the activation of ca2+-activated phospholipid-dependent protein kinase is suggested by the fact that phospholipid b ... | 1983 | 6316349 |
| susceptibility testing of anaerobic bacteria. | 1983 | 6316785 | |
| [pathogenicity of various toxinogenic types of clostridium perfringens administered by mouth to axenic and holoxenic mice]. | axenic mice died with signs of enterotoxaemia after oral ingestion of clostridium perfringens type c or d. under the same conditions, c. perfringens type b was less pathogenic, and types a and e showed no pathogenicity. the microflora of conventional mice prevented the establishment of c. perfringens types b, c and d in the digestive tract and protected them against the pathogenicity of these strains. toxins produced in the caecum of monoxenic mice harbouring c. perfringens type c were not neutr ... | 1983 | 6316828 |
| influence of carbohydrates on growth and sporulation of clostridium perfringens in a defined medium with or without guanosine. | clostridium perfringens strains nctc 8238, nctc 8798, nctc 8679, 8-6, fd-1, and ps52 formed high levels of heat-resistant spores in a defined medium (d) with various sugars as energy sources. strain ps49 formed high levels of heat-resistant spores when grown with dextrin and methylxanthines. the experiments showed the possibility of carrying out experiments on the sporulation of certain c. perfringens strains in a completely defined medium, without using the ill-defined polysaccharide dextrin. t ... | 1983 | 6316852 |
| cyclic amp is not detectable in clostridium perfringens. | cyclic amp was not detected (less than 5 x 10(-9) m intracellular concentration) at any stage of growth or sporulation of two strains of clostridium perfringens grown with or without methylxanthines. only bacillus and lactobacillus, genera belonging to the same phylogenetic cluster, have previously exhibited undetectable levels of cyclic amp. | 1983 | 6317152 |
| purification and some properties of nitrite reductase from clostridium perfringens. | nitrite reductase from clostridium perfringens was purified by chromatographies on deae-cellulose, deae-sephadex, sephadex g-150, and hydroxylapatite and by isoelectric focussing to a homogeneous state, showing essentially a single protein band in disc gel electrophoresis and a single immuno-precipitation line in double diffusion against antiserum obtained from immunized rabbits. the reductase was induced in the presence of nitrate. it had a molecular weight of 54,000 and showed no absorption pe ... | 1983 | 6317661 |
| clostridium perfringens sepsis involving mother and neonate with survival of both. | 1983 | 6318196 | |
| survival of cultured cells after functional and structural disorganization of plasma membrane by bacterial haemolysins and phospholipases. | lesions were induced in the plasma membranes of cultured human fibroblasts by membrane damaging toxins of bacterial origin (haemolysins). structural disorganization of the membrane was measured as leakage of a radiolabelled small cytoplasmic marker and functional membrane damage was measured as decreased uptake of aminoisobutyrate. cell survival was scored 24 and 48 hr later by measuring uptake of trypan blue and by light microscopical evaluation of cell morphology and proliferation. the membran ... | 1983 | 6318394 |
| [bacteriological study of sporadic cases of diarrhea probably due to clostridium perfringens]. | 1983 | 6319507 | |
| influence of diets low in protein or lysine on the cecal flora of rats with reference to cecal contents. | to determine the effect of a certain diet on the intestinal flora of rats, the cecal flora of rats fed a low protein or low lysine diet was examined. total counts of bacteria in the cecal contents of rats given the four kinds of diet (a normal protein diet, a low protein diet, a normal lysine diet and a low lysine diet) were not significantly different. the counts of streptococcus, enterobacteriaceae, and clostridium perfringens in the cecal contents from rats fed the low protein diet were signi ... | 1983 | 6319650 |
| [detection of clostridium perfringens enterotoxin in cases of food poisoning]. | 1983 | 6319951 | |
| enteritis and diarrhea. | 1983 | 6320516 | |
| stimulation of prothrombinase activity of platelets and erythrocytes by sub-lytic treatment with phospholipase c from clostridium welchii. | treatment of platelets or red cells with small amounts of phospholipase c from clostridium welchii enables both cells, prior to the onset of lysis, to stimulate prothrombin conversion by coagulation factor xa and va in the presence of calcium. phospholipase c treatment of both cells also exposes significant amounts of phosphatidylserine at the outer surface. the level of phosphatidic acid formed from diglycerides produced by phospholipase c action, is similar to that formed in activated platelet ... | 1983 | 6320817 |
| susceptibility testing of anaerobic bacteria. | with the significant increase in resistance of anaerobic bacteria to antimicrobial agents in recent years, susceptibility testing of these organisms becomes very important. in addition to survey studies, hospitals should be determining their local patterns, and therapy of at least the more serious infections should be guided by susceptibility tests. of the various tests available, those most suitable for smaller hospitals for guidance of patient management are the broth disc elution procedure an ... | 1983 | 6323094 |
| [relations between food poisoning and ascariasis]. | during the examination of faeces for the causative agents of food poisoning, the search was also extended to the occurrence of worm eggs. ascaris lumbricoides eggs occurred six times more frequently in persons affected with food poisoning than in consumers who were not harmed by the intake of poisoned food. this relationship was observed in non-specific cases of food poisoning and in those of unknown aetiology, whereas it was less pronounced in cases of specific poisoning. this difference has no ... | 1983 | 6322473 |
| studies on the beta-glucuronidase production of clostridia. | we investigated the beta-glucuronidase-producing capacity of 197 strains of 19 different species of clostridia, using the procedure described by kilian and bülow (8). most of the hobbs' serotyped strains of clostridium perfringens (104 out of 109 strains) were found to produce beta-glucuronidase, whereas non-serotyped strains (21 out of 32 strains) did not. some strains of other species such as clostridium beijerinckii (c. beijerinckii), c. clostridiforme, c. difficile, c. limosum, and c. ramosu ... | 1983 | 6326418 |
| use of enzyme linked immunosorbent assay (elisa) in the quantitation of clostridium perfringens type a enterotoxin and antienterotoxin antibodies. | elisa procedure for quantitation of enterotoxin of clostridium perfringens type a was worked out. it was possible to detect as little as 0.0022 microgramme of enterotoxin. when applied to quantitate the enterotoxin in laboratory contaminated faeces and cooked minced beef, elisa detected as little as 0.024 microgramme of enterotoxin. elisa procedure for determination of titre of antibodies to enterotoxin was also developed using hyperimmunised rabbit and sheep sera. it was possible to use this pr ... | 1983 | 6327556 |