Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the dose rate effects on the in vitro radiolysis products of magnesium-guanosine-5'-monophosphate complexes in aqueous solutions. | this study examined the effects on the radiolysis of magnesium-guanosine-5'-monophosphate complexes in deaerated aqueous solutions, investigated by fourier transform infrared (ft-ir) spectroscopy. it was found that when the system was irradiated with a dose rate of 500 rad/min (low dose rate), the oh radicals, h atoms and hydrated electrons (eaq-) produced from radiolysis of water reacted mainly by addition to the double bonds c4 = c5 and n7 = c8 of guanine. when the dose rate was 23,800 rad/min ... | 2007 | 2168727 |
| an analysis of maximum mandibular movements, craniofacial relationships and temporomandibular joint awareness in children. | this paper evaluates the relationship between maximum mandibular vertical opening and the following variables: chronological age, craniomandibular relationships, and temporomandibular joint awareness in children. the range of mandibular movement was evaluated in 189 children between the ages of 4 and 14 years, using the method of agerberg. measurements were found to be accurate and reliable only in the vertical plane. cephalometric tracings were made on 131 of the subjects. significant relations ... | 2007 | 2064068 |
| lead use in california industry: its prevalence and health implications. | an estimated 230,000 workers were reported to work in lead-using activities as a result of a 1986 statistical survey of employers in california. however, 68% of these workers worked in activities of relatively low hazard. this included 114,000 workers engaged in electronic soldering. over 1800 workers were reported to work in battery manufacture, a relatively high-hazard activity, but with consistent monitoring and protection. in contrast, over 4300 workers were involved in radiator repair activ ... | 2007 | 1951050 |
| distinction of mesothelioma from carcinoma in pleural effusions. an immunocytochemical study on routinely processed cytoblock preparations. | the study was designed to find out whether the commercially available antibodies bma 130 c, bma 120, v 9, kl 1, b 72.3 tag, hea 125 and ber-ep 4 would be of help in distinguishing carcinomas from a mesothelial process (mesothelioma/pleuritis) in pleural effusion specimens routinely processed by the cytoblock method. all of the 20 carcinomas included in the study but also 19 of the 20 mesotheliomas expressed cytokeratin (kl 1), whereas vimentin expression was found in 7 of the 20 carcinomas and 1 ... | 2007 | 1876528 |
| lauryl sulphate haemoglobin: a non-hazardous substitute for hicn in haemoglobinometry. | the haemiglobincyanide (hicn) method for measuring haemoglobin is used extensively worldwide; its advantage is the ready availability of a stable and internationally accepted reference standard/calibrator. however, its use may create a problem, especially in automated analysers, as the waste disposal of large volumes of reagent containing cyanide may constitute a potential toxic hazard. as an alternative, conversion of haemoglobin to a sulphate derivative by nontoxic sodium lauryl sulphate has b ... | 2007 | 1794230 |
| intracerebral hemorrhage, cerebral infarction, and subdural hematoma after acute myocardial infarction and thrombolytic therapy in the thrombolysis in myocardial infarction study. thrombolysis in myocardial infarction, phase ii, pilot and clinical trial. | in the thrombolysis in myocardial infarction, phase ii pilot and clinical trial, 908 patients [326 (35.9%) in the pilot study and 582 (64.0%) in the randomized study] were treated with 150 mg recombinant tissue-type plasminogen (rt-pa) activator in combination with heparin and aspirin, and 3,016 patients [64 (2.1%) in the pilot study and 2,952 (97.9%) in the randomized study] were treated with 100 mg rt-pa in combination with heparin and aspirin. adverse neurological events occurred in 23 patien ... | 2007 | 1899364 |
| inbreeding in saguenay-lac-st-jean (quebec, canada): a study of catholic church dispensations 1842-1971. | saguenay-lac-st-jean (slsj) is a rather geographically isolated region of quebec which shows a high occurrence of hereditary disorders. it has been suggested that high inbreeding might explain this situation. we studied the inbreeding in the slsj region by 10-year periods from 1842 till 1971 using the catholic church dispensations. the values of the mean inbreeding coefficient were found to be low during the whole period, reaching a peak of 22.94 x 10(-4) during the period 1902-1911. the values ... | 2007 | 1797631 |
| on talc, tremolite, and tergiversation. ter-gi-ver-sate: 2: to use subterfuges. | 2007 | 2039750 | |
| [membranous glomerulonephritis. experience at the instituto nacional de la nutrición salvador zubirán]. | the purpose of this retrospective study was to study the incidence of idiopathic and secondary forms of membranous nephropathy in our institution, its clinical course and progression to chronic renal failure, and the risk factors associated with it. two hundred fourteen (16%) of the 1,287 renal biopsies obtained between 1962 and 1988 were primary glomerular diseases and 28 of this 214 (13%) were idiopathic membranous nephropathy. on the other hand 59 of 1,287 biopsies were membranous nephropathy ... | 2007 | 2267443 |
| illumination and errors in dispensing. | the relationship between the level of illumination and the prescription-dispensing error rate in a high-volume army outpatient pharmacy was investigated. the prescription error rate was determined by direct, undisguised observation and retrospective prescription review under three levels of illumination (45, 102, and 146 foot-candles) during 21 consecutive weekdays. illumination was controlled in the prescription-checking area of the pharmacy by using additional fluorescent lamps and filters. th ... | 2007 | 1781468 |
| [retrospective epidemiologic analysis of the occurrence of measles in slovakia]. | the authors give a detailed account of the reported measles morbidity in slovakia during the past 50 years (almost half a million notified cases). before vaccination on a mass scale was introduced, some 1% of the notified cases died. the most frequent cause of death was pneumonia, followed by encephalitis. since 1977 there was not a single death from measles. before vaccination was introduced preschool children were affected most badly by the morbidity. after introduction of vaccination on a mas ... | 2007 | 1828717 |
| [internal tooth resorption]. | 2007 | 2206646 | |
| [laser coagulation of the cornea with a holmium:yag laser for correction of hyperopia]. | we present a new technique of hyperopic correction similar to radial thermokeratoplasty. a pulsed ho: yag laser was used that emitted radiation at 2.06 microns and was guided by a quartz fiber and a focusing hand piece. the penetration depth of this light in the cornea is about 400 microns. eight or 16 concentric point coagulations result in steepening in the central cornea. this refractive change is inverse related to the distance to the cornea center, and it increases linearly with increasing ... | 2007 | 1855726 |
| [laser coagulation of the cornea with a holmium:yag laser for correction of hyperopia]. | we present a new technique of hyperopic correction similar to radial thermokeratoplasty. a pulsed ho: yag laser was used that emitted radiation at 2.06 microns and was guided by a quartz fiber and a focusing hand piece. the penetration depth of this light in the cornea is about 400 microns. eight or 16 concentric point coagulations result in steepening in the central cornea. this refractive change is inverse related to the distance to the cornea center, and it increases linearly with increasing ... | 2007 | 1855726 |
| response by dr. alden n. haffner upon receipt of the koch memorial medal 1990. | 2007 | 1852404 | |
| mammography quality assurance. | 2007 | 1950889 | |
| alpha-fetoprotein. | the past year has seen major challenges to existing maternal serum alpha-fetoprotein testing protocols for both neural tube defects and chromosomal anomalies. these challenges are reviewed along with the physiology of alpha-fetoprotein; the use of amniocentesis, ultrasonography, and additional serum markers in women with elevated alpha-fetoprotein levels; and epidemiologic implications of maternal serum alpha-fetoprotein screening. | 2007 | 1717020 |
| alpha-fetoprotein. | the past year has seen major challenges to existing maternal serum alpha-fetoprotein testing protocols for both neural tube defects and chromosomal anomalies. these challenges are reviewed along with the physiology of alpha-fetoprotein; the use of amniocentesis, ultrasonography, and additional serum markers in women with elevated alpha-fetoprotein levels; and epidemiologic implications of maternal serum alpha-fetoprotein screening. | 2007 | 1717020 |
| lightness, embeddedness, and contrast. | 2007 | 5054071 | |
| [the topographic anatomical basis for ventriculosinusal shunting in hydrocephalus in nursing and young infants]. | the peculiarities in the structure and topographic anatomical relations of the superior longitudinal, lateral, and sigmoid sinuses were studied on block preparations of the posterior cranial fossa of 14 children under 3 years of age who died from somatic diseases. it was shown that intrasinus inclusions preventing catheterization of the sinus (bands and septa) were absent at the junction of the first angle of the sigmoid sinus with its descending part. the authors recommend this area for shuntin ... | 2007 | 2168650 |
| hybridocytochemistry with non-radioactive probes as a tool for biomedical research and cytodiagnostics. | the advent of dna and rna labelling techniques which allow non-autoradiographic detection of specific nucleic acid sequences has opened new possibilities for rapid diagnosis at the nucleic acid level. there are already practical applications. however, in order to fully compete with radioactive procedures an increase in sensitivity is still desired. | 2007 | 2435580 |
| [derivative spectroscopy and its use in sanitary hygiene control]. | an analytical method is developed for defining the residual quantities of rodenticide "brodifacoum" (talon) used in the control of harmful field rodents and especially for species microtus. the method is based on extracting the pesticide with chloroform and making use of spectrophotometry in the ultraviolet field in intervals 340-240 nm. the second derivative of the absorption is utilized as well as the band with maximum 288 nm and minimum 228 nm. the peak height in this interval is in proportio ... | 2007 | 2099444 |
| regulation of alcohol consumption by the renin-angiotensin system: a review of recent findings and a possible mechanism of action. | the renin-angiotensin system has traditionally been associated with the regulation of fluid and electrolyte balance. in this review we summarize the data which ascribes a completely new function to this system, i.e., the regulation of alcohol consumption. in addition, we suggest a possible mechanism for this effect based on the concept of a satiety or stop process. the approach taken was to examine the effect on alcohol intake of a wide variety of drug, genetic, dietary, surgical and neurosurgic ... | 2007 | 1852316 |
| discordant expression of tuberous sclerosis in monozygotic twins. | a set of 20-year-old female japanese twins, most probably monozygotic, had clinical evidence of tuberous sclerosis (ts). they were discordant for symptoms. one of the twins exhibited facial red-brown papules (adenoma sebaceum), a dorsal shagreen patch, intracerebral calcifications, angiomyolipoma in the right kidney, and hypopigmented macules; the other had only a few hypopigmented macules. modification of ts gene expression by the effects of environmental (extrinsic) factors is suggested. | 2007 | 1885853 |
| [brain stem auditory evoked potentials in the early period after a brain concussion]. | acoustic truncal evoked potentials (atep) were studied in 24 patients in the first 48 hours after concussion of the brain. the absolute peak latency of components 1, 3, and 5, the interpeak intervals 1-3, 3-5, and 1-5, and the interaural difference were determined. the results were compared with those of examination of a control group of 24 healthy individuals. individual and group appraisal failed to reveal any significant differences from normal values in none of the studied parameters in pati ... | 2007 | 2168645 |
| [insulin treatment of diabetes mellitus]. | 2007 | 2258670 | |
| [insulin treatment of diabetes mellitus]. | 2007 | 2258670 | |
| [difficulties in the diagnosis of primary malignant histiocytoma of the lung]. | a case is presented of a round coin shadow seen on a routine chest radiogram producing grave diagnostic problems. during hospitalization the patient presented neurological and bone and joint involvement symptoms. transthoracic needle biopsy of the lesion allowed to diagnose large cell cancer, but autopsy changed this diagnosis to malignant histiocytoma. | 2007 | 2168043 |
| [hyperoxalemia and secondary oxalosis of chronic renal insufficiency]. | 2007 | 2074920 | |
| [clinical significance of the determination of serum fructosamine and albumin in children with diabetes mellitus]. | the paper is devoted to a study of the role of serum glycoprotein fructosamine and serum albumin in the pathogenesis of a severe course of insulin dependent diabetes mellitus (iddm) in children. fructosamine was determined in 43 pediatric patients with iddm by direct spectrophotometry using hoffman-la-roche kits; albumin, c-peptide and malonic aldehyde were also determined. disorder of the mechanism of regulation of homeostasis by albumin was shown to play an important role in the pathogenesis o ... | 2007 | 2717569 |
| surgical aortic valvuloplasty using the cavitron ultrasonic surgical aspirator: an invasive hemodynamic follow-up study. | valve repair and calcium debridement in patients with calcific aortic stenosis, using the cavitron ultrasonic surgical aspirator (cusa), results in a reduction in the aortic valve gradient while potentially avoiding long-term problems inherent to prosthetic valves. invasive followup data in these patients has not previously been reported. ten patients in whom cusa debridement was performed underwent cardiac catheterization prior to and 8.0 +/- 2.5 months following the procedure. compared to base ... | 2007 | 1913786 |
| [age determination of venous thrombi by ultrasound]. | in addition to diagnosing deep-vein thrombosis of the lower limb and pelvis, determination of the age of the thrombus is of considerable importance in guiding treatment. the present study was done to find out whether thrombus age can be determined by ultrasound morphology. 138 examinations were carried out on 76 lower limbs with phlebographically demonstrated thrombosed veins. diameter of the thrombosed vein, echogenicity of the thrombus and demarcation of the venous wall from its surroundings w ... | 2007 | 1932731 |
| an entrepreneurial role for the nurse consultant. | 2007 | 2725970 | |
| [opinions and attitudes toward dentistry in early adolescence]. | 2007 | 1852655 | |
| how we teach ... practical skills in an undergraduate medical curriculum: 5 years of experience at gezira medical school (sudan). | an outline of an undergraduate course to teach medical students practical medical procedures early in their curriculum is described. introduction of those skill as early as the second year has given the student ample time to practice those skills before graduation. feedback has shown that the course has been successful in helping the students to master simple procedures by the time they graduate. early demonstration of complicated skills has also facilitated the ability and confidence of the stu ... | 2007 | 1865801 |
| [induction of lung maturation in the early stage of premature rupture of fetal membranes]. | 2007 | 1790890 | |
| utility of leopold maneuvers in screening for malpresentation. | malpresentation is a common complication of pregnancy. using ultrasound as the gold standard, we prospectively tested leopold maneuvers as a screen for nonvertex presentation in the third trimester. in our hands this technique performed poorly. values for sensitivity, specificity, positive predictive value, and negative predictive value were 28, 94, 24, and 95%, respectively, in a population with a 7% prevalence of noncephalic presentation. much of the correlation between the screening and diagn ... | 2007 | 1876372 |
| [growth and therapy in angle class ii/2--a longitudinal cephalometric study]. | the influence of both growth and functional therapy with the u-bow-activator type i on angle class ii subdivision 2 malocclusion is analysed in a cephalometric long term study. findings concerning skeletal problems: 1. therapeutically intended clockwise rotation of the mandible with vertically and sagittally controlled maxillary position, 2. achieving normal sagittal mandibular growth rates. dental problems are improved significantly. the results obtained by functional therapy and growth proved ... | 2007 | 2227736 |
| [growth and therapy in angle class ii/2--a longitudinal cephalometric study]. | the influence of both growth and functional therapy with the u-bow-activator type i on angle class ii subdivision 2 malocclusion is analysed in a cephalometric long term study. findings concerning skeletal problems: 1. therapeutically intended clockwise rotation of the mandible with vertically and sagittally controlled maxillary position, 2. achieving normal sagittal mandibular growth rates. dental problems are improved significantly. the results obtained by functional therapy and growth proved ... | 2007 | 2227736 |
| [lung cancer in women in moscow (epidemiology, etiology and surgical treatment)]. | in the studied period (1973-1987) the lung carcinoma morbidity rate among females of moscow, in general intensive indices (per 100,000 females), showed a tendency to reduce (18.4 in 1973 and 15.1 in 1987). a reverse tendency is mostly encountered in other countries. more than half of patients with lung carcinoma were over 70 years of age. the ratio of males with lung carcinoma to that of females grew from 3.0:1.0 in 1973-1977 to 3.6:1.0 in 1983-1987. in the period from 1979 to 1988, 380 females ... | 2007 | 2223213 |
| [lung cancer in women in moscow (epidemiology, etiology and surgical treatment)]. | in the studied period (1973-1987) the lung carcinoma morbidity rate among females of moscow, in general intensive indices (per 100,000 females), showed a tendency to reduce (18.4 in 1973 and 15.1 in 1987). a reverse tendency is mostly encountered in other countries. more than half of patients with lung carcinoma were over 70 years of age. the ratio of males with lung carcinoma to that of females grew from 3.0:1.0 in 1973-1977 to 3.6:1.0 in 1983-1987. in the period from 1979 to 1988, 380 females ... | 2007 | 2223213 |
| [lipoprotein (a)--a further risk factor in arteriosclerosis?]. | epidemiological studies have identified lipoprotein (a), which has been known since 1963, but has received attention only recently, as a further risk factor for premature arteriosclerosis. this substance is similar to low-density lipoprotein, but it's serum concentration (and thus the increase in risk) is genetically determined and highly variable from one individual to another. the usual dietary and drug measures have only little effect on lipoprotein (a) serum levels. although the physiologica ... | 2007 | 2147016 |
| ovarian cancer. | 2007 | 2042058 | |
| [long-term results of the treatment of ruptured intracranial aneurysms. study of a series of 328 patients hospitalized from 1972 to 1984]. | between 1972 to 1984, 328 patients were admitted in the department of neurosurgery, for the treatment of a ruptured intracranial aneurysm, and being clinically in grades i to iv. these patients were submitted to a deferred surgery protocol. 5.5% of the patients died before surgery, and 94.5% were actually operated on. the follow-up was at least over one year, and up to 14 years (mean 3 years and 8 months). the long term result was appreciated according to the physical status of the patients and ... | 2007 | 3374722 |
| access to information for adult adoptees. | 2007 | 1913424 | |
| paraquat poisoning with acute renal failure--a case report. | paraquat poisoning is relatively rare and is associated with mortality varying from 35 to 50%. a patient who consumed paraquat developed features of non-oliguric acute renal failure and recovered following haemodialysis. renal biopsy done during the early recovery phase showed features of acute tubulo-interstitial nephritis with no disruption of tubular basement membrane. on recovery the patient had no evidence of proximal renal tubular dysfunction. | 2007 | 2613639 |
| [transient polyuria in pregnancy in diabetes insipidus and gestational diabetes]. | two pregnant women developed overt polyuria (up to 11 l/day) and polydipsia during their second and third trimesters of pregnancy. in one patient hydronephrosis was present. both patients suffered from mild gestational diabetes mellitus. plasma sodium was 145 and 162 mmol/l. polyuria and urinary hypo-osmolality responded well to desmopressin acetate. after delivery, polyuria and polydipsia disappeared in one patient and significantly improved in the other. infusion of hypertonic saline one and t ... | 2007 | 1770904 |
| acute intoxication as a cause of wild bird mortality in the netherlands from 1975-1989. | 2007 | 1805755 | |
| [initial attitudes to orthodontic treatment--the results of a practical and clinical questionnaire study (i)]. | 312 patients from one clinic and nine offices, between eight and 15 years of age treated with bionators, their parents and a control group were questioned on various aspects of their treatment. findings regarding opinions about dentofacial appearance, initial motives and expectations are presented. aesthetic motives proved preponderant by seeking treatment though the children's ability to verbally differentiate aesthetic concepts was poor. above all, they expected visible treatment progress, abs ... | 2007 | 2227739 |
| [initial attitudes to orthodontic treatment--the results of a practical and clinical questionnaire study (i)]. | 312 patients from one clinic and nine offices, between eight and 15 years of age treated with bionators, their parents and a control group were questioned on various aspects of their treatment. findings regarding opinions about dentofacial appearance, initial motives and expectations are presented. aesthetic motives proved preponderant by seeking treatment though the children's ability to verbally differentiate aesthetic concepts was poor. above all, they expected visible treatment progress, abs ... | 2007 | 2227739 |
| renal damage in mice after sequential cisplatin and irradiation: the influence of prior irradiation on platinum elimination. | doses of 4-6 mg kg-1 c-ddp given 6 months before renal irradiation caused only a modest increase in functional radiation damage (def 1.1). these effects could be explained by additive toxicities and the damage was much less than when c-ddp was given 3-6 months after irradiation. pharmacokinetic studies did not demonstrate any decrease in the rate of platinum elimination after previous low-dose renal irradiation. | 2007 | 1924867 |
| effect of macrophages on interleukin-2 (il-2)- and il-4-induced murine lymphokine-activated killer activity. | the enhancing effect of macrophages on interleukin-2 (il-2)- and il-4-induced murine lymphokine-activated killer (lak) activity was investigated in this study. peritoneal macrophages significantly enhanced lak activity generated from accessory cell-depleted splenic lymphocytes in both il-2 and il-4 cultures. this effect was dependent on the number of macrophages and was not replaced by a factor derived from macrophages or lymphocytes. macrophages enhanced il-2- and il-4-induced lak activity agai ... | 2007 | 2045201 |
| [diagnosis and surgical treatment of traumatic damage to the sinus of valsalva following penetrating wounds of the heart]. | the authors had three patients with traumatic damage of the sinus of valsalva, caused by penetrating injury of the thorax inflicted with a knife. the patients were brought to the clinic in a grave condition with decompensation of systemic and pulmonary circulation. the anomaly called for emergency surgical correction under conditions of extracorporeal circulation. the diagnosis was based on the complaints, medical history, clinical picture, the results of functional methods of examination includ ... | 2007 | 2223226 |
| [diagnosis and surgical treatment of traumatic damage to the sinus of valsalva following penetrating wounds of the heart]. | the authors had three patients with traumatic damage of the sinus of valsalva, caused by penetrating injury of the thorax inflicted with a knife. the patients were brought to the clinic in a grave condition with decompensation of systemic and pulmonary circulation. the anomaly called for emergency surgical correction under conditions of extracorporeal circulation. the diagnosis was based on the complaints, medical history, clinical picture, the results of functional methods of examination includ ... | 2007 | 2223226 |
| [a congenital esophagotracheal fistula in an adult]. | 2007 | 2223222 | |
| [a congenital esophagotracheal fistula in an adult]. | 2007 | 2223222 | |
| [a juvenile form of osteochondrosis]. | 2007 | 2074966 | |
| occult rupture of spleen. | 2007 | 1800517 | |
| scalp cooling by cold air for the prevention of chemotherapy-induced alopecia. | a new system is described for cooling the scalp with cold air in order to prevent chemotherapy-induced alopecia. compressed air was cooled by means of a vortex tube built into a hair-drier cap. this system reduced the blood flow in the scalp to 35%, the surface temperature to 14.2 degrees c and the intradermal temperature at hair follicle level to 29.2 degrees c. the low temperature could be kept constant for at least one hour of cooling. by means of comparison, with cryogel packs the lowest epi ... | 2007 | 2074916 |
| isolation and characterization of pathogenic vibrio parahaemolyticus from diseased post-larvae of abalone haliotis diversicolor supertexta. | mass mortality among the post-larvae of cultured abalone haliotis diversicolor supertexta has occurred on the south coast of china since 2002. the diseased abalone are generally 10 to 30 days old, and typical signs of the disease include them turning white in colour and falling off the diatom films on which they were cultured. among sixteen different motile bacteria isolated from the diseased post-larvae, four were identified as vibrio parahaemolyticus on the basis of biochemical characteristics ... | 2007 | 17304623 |
| comparison of different primers for rapid detection of vibrio parahaemolyticus using the polymerase chain reaction. | the aim of this study was to compare different primers for rapid and effective detection of vibrio parahaemolyticus by polymerase chain reaction (pcr). | 2007 | 17309499 |
| o3:k6 serotype of vibrio parahaemolyticus identical to the global pandemic clone associated with diarrhea in peru. | to determine if the vibrio parahaemolyticus o3:k6 global pandemic clone has spread into peru. | 2007 | 17321179 |
| occurrence of vibrio spp. in blue mussels (mytilus edulis) from the german wadden sea. | the present study aimed at determining the contamination of blue mussels grown in germany with relevant bacterial organisms. seasonal variations were examined. for that purpose, mussel samples were taken regularly between june 2004 and may 2005 in seven shellfish-growing areas of the german wadden sea. a total of 90 samples were analysed. the analysis included the compulsory microbiological parameters escherichia coli and salmonella spp., as well as the determination of the contamination with vi ... | 2007 | 17331605 |
| purification and characterization of vibrio parahaemolyticus extracellular chitinase and chitin oligosaccharide deacetylase involved in the production of heterodisaccharide from chitin. | a chitin-degrading bacterial strain, kn1699, isolated from yatsu dry beach (narashino, chiba prefecture, japan), was identified as vibrio parahaemolyticus. treatment of powdered chitin with crude enzyme solution prepared from the supernatant of kn1699 cultures yielded a disaccharide, beta-d-n-acetylglucosaminyl-(1,4)-d-glucosamine (glcnac-glcn), as the primary chitin degradation product. the extracellular enzymes involved in the production of this heterodisaccharide, chitinase (pa-chi; molecular ... | 2007 | 17334758 |
| evaluation of a tissue culture-based approach for differentiating between virulent and avirulent vibrio parahaemolyticus strains based on cytotoxicity. | the ability of only a subset of vibrio parahaemolyticus strains to cause human infection underscores the need for an analytical method that can effectively differentiate between pathogenic strains and those that do not cause disease. we tested the feasibility of a tissue culture-based assay to determine whether clinical isolates could be differentiated from nonclinical isolates based on relative isolate cytopathogenicity. to screen for cytotoxic capability, we measured relative extracellular lac ... | 2007 | 17340868 |
| effects of heat-degraded sugars on survival and growth of vibrio parahaemolyticus and other bacteria. | we studied the effects of autoclaved (121 degrees c, 15 min) sugar solutions on the survival and growth of vibrio parahaemolyticus and other bacteria. the growth and survival of v. parahaemolyticus in luria-bertani media and phosphate buffer, respectively, were inhibited by the addition of d-glucose autoclaved in ph 8.0 phosphate buffer. the bactericidal effect of autoclaved d-glucose was very small when autoclaved in ph 7.0 phosphate buffer, but larger effects were observed when autoclaved in t ... | 2007 | 17340871 |
| expression, characterization and immunogenicity of a major outer membrane protein from vibrio alginolyticus. | vibrio alginolyticus is one of the vibrio pathogens common to humans and marine animals. during infection and induction of the host immune response, outer membrane proteins of bacteria play an important role. in this study, an outer membrane protein gene (ompw) was cloned from v. alginolyticus and expressed in escherichia coli. the 645 bp open reading frame (orf) encodes a protein of 214 amino acid residues with a predicted molecular weight of 23.3 kda. the amino acid sequence showed a high iden ... | 2007 | 17342258 |
| profile of acute immune response in chinese amphioxus upon staphylococcus aureus and vibrio parahaemolyticus infection. | amphioxus is traditionally considered as the living invertebrate most closely related to vertebrate. however, no systematic study was performed about how the amphioxus defends against the microbial invasion. here we reported a profile of gene transcription after staphylococcus aureus (s.c) and vibrio parahaemolyticus (v.p) challenged by suppression subtractive hybridization (ssh). when compared with mammals, amphioxus has the same acute immune defense genes (lectins, metalloproteinase, lysozymes ... | 2007 | 17343913 |
| variability of properties of vibrio parahaemolyticus strains isolated from individual patients. | infections by strains belonging to the o3:k6 pandemic clone of vibrio parahaemolyticus are prevalent in southern thailand, and serovariants of these strains have also been detected. v. parahaemolyticus strains lacking important virulence genes (tdh and trh) were isolated from 6.5 to 10.9% of clinical specimens during the period from 2000 to 2003. in order to understand whether changes to the characteristics of v. parahaemolyticus occur during infection, 10 isolates collected from each of 63 pati ... | 2007 | 17344357 |
| comment on "vibrio vulnificus and v. parahaemolyticus necrotising fasciitis in fishermen visiting an estuarine tropical northern australia location". | 2007 | 17350104 | |
| studies on the region involved in the transport activity of escherichia coli tolc by chimeric protein analysis. | gram-negative bacteria possess the outer membrane protein tolc which acts as an exit duct across the outer membrane. however, the region involved in the transport activity of tolc has remained unclear. we analyzed this region by creating chimeric tolcs. first, we expressed the genes for tolcs of vibrio parahaemolyticus (vp-tolc) and salmonella typhimurium (sal-tolc) in escherichia coli. the levels of sequence identity in the mature region of vp-tolc/ec-tolc and sal-tolc/ec-tolc with maximum matc ... | 2007 | 17350794 |
| inactivation of foodborne pathogens using a one atmosphere uniform glow discharge plasma. | this study was conducted to determine the efficacy of a one atmosphere uniform glow discharge plasma (oaugdp) for inactivation of foodborne pathogens and to evaluate the influence of growth temperature, ph, and culture age on their inactivation. escherichia coli o157:h7, listeria monocytogenes, staphylococcus aureus, bacillus cereus, salmonella enteritidis, vibrio parahaemolyticus, yersinia enterocolitica, and shigella flexneri were evaluated. three-strain mixtures of each bacterium were inocula ... | 2007 | 17378708 |
| growth response of vibrio cholerae and other vibrio spp. to cyanobacterial dissolved organic matter and temperature in brackish water. | environmental control of growth and persistence of vibrios in aquatic environments is poorly understood even though members of the genus vibrio are globally important pathogens. to study how algal-derived organic matter and temperature influenced the abundance of different vibrio spp., baltic sea microcosms inoculated with vibrio cholerae, vibrio vulnificus, vibrio parahaemolyticus, vibrio alginolyticus and native bacterioplankton, were exposed to different temperatures (12-25 degrees c) and ame ... | 2007 | 17386033 |
| adrenergic regulation of bacterial virulence. | 2007 | 17396992 | |
| modulation of pathogenicity with norepinephrine related to the type iii secretion system of vibrio parahaemolyticus. | norepinephrine (ne) controls the functions of the gastrointestinal tract, but its role in the pathogenicity of enteropathogens is not clear. we examined the effect of ne on the pathogenicity of vibrio parahaemolyticus with regard to its type iii secretion systems (ttsss). | 2007 | 17397007 |
| scrg, a ggdef-eal protein, participates in regulating swarming and sticking in vibrio parahaemolyticus. | in this work, we describe a new gene controlling lateral flagellar gene expression. the gene encodes scrg, a protein containing ggdef and eal domains. this is the second ggdef-eal-encoding locus determined to be involved in the regulation of swarming: the first was previously characterized and named scrabc (for "swarming and capsular polysaccharide regulation"). ggdef and eal domain-containing proteins participate in the synthesis and degradation of the nucleotide signal cyclic di-gmp (c-di-gmp) ... | 2007 | 17400744 |
| vibrio parahaemolyticus: a concern of seafood safety. | vibrio parahaemolyticus is a human pathogen that is widely distributed in the marine environments. this organism is frequently isolated from a variety of raw seafoods, particularly shellfish. consumption of raw or undercooked seafood contaminated with v. parahaemolyticus may lead to development of acute gastroenteritis characterized by diarrhea, headache, vomiting, nausea, and abdominal cramps. this pathogen is a common cause of foodborne illnesses in many asian countries, including china, japan ... | 2007 | 17418305 |
| the dominance of pandemic serovars of vibrio parahaemolyticus in expatriates and sporadic cases of diarrhoea in thailand, and a new emergent serovar (o3 : k46) with pandemic traits. | vibrio parahaemolyticus is a major cause of gastroenteritis worldwide. a total of 95 v. parahaemolyticus isolates belonging to 23 different serovars were identified in a case-control study of expatriates and thai adults from 2001 to 2002 in thailand. fifty-two per cent of isolates (49/95) were resistant to ampicillin and sulfisoxazole, but all isolates were susceptible to ciprofloxacin and trimethoprim-sulfamethoxazole, two antibiotics commonly used to treat traveller's diarrhoea. all isolates w ... | 2007 | 17446282 |
| [distribution of thermostable direct hemolysin (tdh)- and tdh-related hemolysin (trh)-producing vibrio parahaemolyticus in coastal shimane prefecture and tdh and trh v parahaemolyticus contamination of retail shellfish]. | we studied distribution of thermostable direct hemolysin (tdh)- and tdh-related hemolysin (trh)-producing vibrio parahaemolyticus in coastal sea water, sediment, and shellfish and related retail shellfish contamination in shimane prefecture, japan, between 2002 and 2004. v. parahaemolyticus was isolated from > 80% of sea water, sediment, and shellfish. the detection of tdh gene (tdh) and trh gene (trh)-positive v parahaemolyticus in sea water was 11%, in sediment 16%, and in shellfish 26%. the n ... | 2007 | 17447472 |
| cloning, expression and immunogenicty analysis of five outer membrane proteins of vibrio parahaemolyticus zj2003. | genes of five outer membrane proteins of vibrio parahaemolyticus zj2003, including ompw, ompv, ompk, ompu and tolc, were cloned and expressed as n-terminal his(6)-tagged proteins in escherichia coli. the recombinant fusion proteins were purified with nickel chelate affinity chromatography. to analyze the immunogenicity of these proteins, large yellow croaker (pseudosciaena crocea) were immunized by intraperitoneal injection. antibody response was assessed by method of enzyme-linked immunosorbent ... | 2007 | 17451968 |
| an evaluation of the use of remotely sensed parameters for prediction of incidence and risk associated with vibrio parahaemolyticus in gulf coast oysters (crassostrea virginica). | the u.s. food and drug administration recently published a vibrio parahaemolyticus risk assessment for consumption of raw oysters that predicts v. parahaemolyticus densities at harvest based on water temperature. we retrospectively compared archived remotely sensed measurements (sea surface temperature, chlorophyll, and turbidity) with previously published data from an environmental study of v. parahaemolyticus in alabama oysters to assess the utility of the former data for predicting v. parahae ... | 2007 | 17477256 |
| characteristics of virulent vibrio parahaemolyticus isolated from oregon and washington. | thirty-four virulent strains of vibrio parahaemolyticus containing tdh and/or trh genes isolated from oregon and washington coastal water were analyzed for o-group antigens and urease activity, and by pulsed-field gel electrophoresis. six o serotypes (o1, o3, o4, o5, o10, and o11) were identified among the isolates, with the o5 group (19 isolates) being the most prevalent, followed by the 01 group (9 isolates). nearly all (33 of 34) isolates were capable of producing urease, which reaffirmed the ... | 2007 | 17477276 |
| production of a recombinant chitin oligosaccharide deacetylase from vibrio parahaemolyticus in the culture medium of escherichia coli cells. | an open reading frame (orf) encoding chitin oligosaccharide deacetylase (pa-cod) gene and its signal sequence was cloned from the vibrio parahaemolyticus kn1699 genome and its sequence was analyzed. the orf encoded a 427 amino acid protein, including the 22 amino acid signal sequence. the deduced amino acid sequence was highly similar to several bacterial chitin oligosaccharide deacetylases in carbohydrate esterase family 4. an expression plasmid containing the gene was constructed and inserted ... | 2007 | 17479220 |
| microbiological study of cooked crustaceans and molluscan shellfish from uk production and retail establishments. | a study of cooked crustaceans and molluscan shellfish from retail and production premises was undertaken in the uk during 2003 to determine the microbiological safety of these products. according to criteria in european commission recommendation 2003/10/ec, 96% of 682 batches were of satisfactory/acceptable microbiological status while 4% of batches were unsatisfactory due to the presence of high levels of escherichia coli (3.3%; 10(2) to 10(6) cfu g(-1)), vibrio parahaemolyticus (0.4%; 10(2) to ... | 2007 | 17479385 |
| [characteristics of vibrio parahaemolyticus isolated from mussels (perna perna) commercialized at niterói, rio de janeiro]. | the marine ecosystem is the natural habitat of bacteria like vibrio parahaemolyticus, an important pathogen that cause human gastroenteritis associated with seafood consumption. in the present investigation, the presence of v. parahaemolyticus in 86 in natural and precooked mussel samples was evaluated. vibrio parahaemolyticus was isolated from 11.6% of the in natural and precooked mussels. all strains tested were urease-positive and 28.5% were kanagawa-positive, which suggests that they have pa ... | 2007 | 17486255 |
| an efficient system for markerless gene replacement applicable in a wide variety of enterobacterial species. | we describe an improved allelic-exchange method for generating unmarked mutations and chromosomal dna alterations in enterobacterial species. initially developed for use in salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. we have extended its use into related bacterial species that are more recalcitrant to genetic manipulations, including enterohemorrhagic and enteropathogenic escherichia coli and vibrio parahaemolyticus. data from over 50 experiments ... | 2007 | 17496950 |
| new role of c-terminal 30 amino acids on the insoluble chitin hydrolysis in actively engineered chitinase from vibrio parahaemolyticus. | a chitinase (vpchia) and its c-terminal truncated g589 mutant (vpchiag589) of vibrio parahaemolyticus were cloned by polymerase chain reaction (pcr) techniques. to study the role of the c-terminal 30 amino acids of vpchia in the enzymatic hydrolysis of chitin, both the recombinant vpchia and vpchiag589 encoded in 1,881 and 1,791 bp dna fragments, respectively, were expressed in escherichia coli using the pet-20b(+) expression system. the his-tag affinity purified vpchia and vpchiag589 enzymes ha ... | 2007 | 17508209 |
| vibrio parahaemolyticus strains isolated during investigation of the summer 2006 seafood related diarrhea outbreaks in two regions of chile. | nine hundred cases of seafood related diarrhea were reported in the region of puerto montt, chile during the austral summer of 2006. this is the continuation of the large outbreaks associated with the consumption of seafood containing the vibrio parahaemolyticus serovar o3:k6 pandemic clonal group that arose last decade in chile. the initial outbreaks occurred during the summer of 1998 in antofagasta (23 degrees 39's 70 degrees 24'w). subsequently, outbreaks there were rare, but since 2004 outbr ... | 2007 | 17521760 |
| detection of tdh and trh genes in vibrio parahaemolyticus isolated from corbicula moltkiana prime in west sumatera, indonesia. | the occurrence of vibrio parahaemolyticus in raw corbicula moltkiana prime from lake singkarak and pasar raya padang market and in cooked samples in west sumatera, indonesia, was studied. thirteen raw and seven cooked bivalve samples were positive using chromagar vibrio medium. all 47 v parahaemolyticus isolates were positive for toxr gene but negative for trh. however, 36% (17/47) of v parahaemolyticus strains were positive for tdh gene. antibiotic profiling showed that 76% and 38% of isolates ... | 2007 | 17539286 |
| effect of the addition of four potential probiotic strains on the survival of pacific white shrimp (litopenaeus vannamei) following immersion challenge with vibrio parahaemolyticus. | four bacterial strains isolated from the gastrointestinal tract of adult shrimp litopenaeus vannamei, vibrio alginolyticus utm 102, bacillus subtilis utm 126, roseobacter gallaeciensis slv03, and pseudomonas aestumarina slv22, were evaluated for potential use as probiotics for shrimp. in vitro studies demonstrated antagonism against the shrimp-pathogenic bacterium, vibrio parahaemolyticus ps-017. feeding shrimp with diets containing the potential probiotics showed the best feed conversion ratio ... | 2007 | 17544437 |
| real-time reverse transcription-pcr for transcriptional expression analysis of virulence and housekeeping genes in viable but nonculturable vibrio parahaemolyticus after recovery of culturability. | a real-time reverse transcription-pcr method was developed to determine whether the recovery of culturability of viable but nonculturable (vbnc) vibrio parahaemolyticus induced the expression of virulence genes coding for the thermostable direct hemolysin and for type iii secretion system 2 (ttss2). the culturability of clinical strain vp5 of v. parahaemolyticus in artificial seawater at 4 degrees c was monitored, and the vbnc state was obtained. one day after entry into the vbnc state, temperat ... | 2007 | 17557845 |
| vibrio parahaemolyticus o3:k6 epidemic diarrhea, chile, 2005. | 2007 | 17561569 | |
| distribution, genetic richness and phage sensitivity of vibrio spp. from coastal british columbia. | this study examined the distribution, susceptibility to viral infection and genetic diversity of vibrio spp. in the coastal waters and sediments of british columbia during summer (july and august). abundances of presumptive vibrio spp. ranged from 1.5 to 346 ml(-1) within the water column (1-291 m); whereas, abundances at the water-sediment interface were much higher (up to approximately 3 x 10(4)vibrio spp. cc(-1)), and decreased with sediment depth (down to 30 cm). the genetic diversity of vib ... | 2007 | 17564612 |
| catecholamine-induced stimulation of growth in vibrio species. | to evaluate the effect of norepinephrine (ne) and related compounds on the growth of bacteria, we have examined the effect of the neuroendocrine hormone ne and related compounds on the growth of vibrio parahaemolyticus and other human-pathogenic vibrio species (vibrio cholerae, vibrio vulnificus, and vibrio mimicus). | 2007 | 17576228 |
| vibrios of the spotted rose snapper lutjanus guttatus steindachner, 1869 from northwestern mexico. | to characterize and identify vibrios present in wild and cultured juvenile snappers (lutjanus guttatus) in northwestern mexico. | 2007 | 17578416 |
| comparison of four antibiotics with indigenous marine bacillus spp. in controlling pathogenic bacteria from shrimp and artemia. | use of antibiotics for the control of bacterial diseases in shrimp culture has caused several adverse impacts to the industry. this has resulted in the search for alternative environment friendly approaches to overcome bacterial infections. this study was conducted to investigate the use of beneficial bacteria as an alternative to antibiotics. ten pathogenic bacterial species isolated from shrimp, penaeus monodon, and artemia cysts were tested for susceptibility to indigenous marine bacillus sub ... | 2007 | 17584435 |
| isolation, sequencing and characterization of cluster genes involved in the biosynthesis and utilization of the siderophore of marine fish pathogen vibrio alginolyticus. | in fish pathogen vibrio alginolyticus mvp01, the isolated 11-gene cluster consisted of two divergently transcribed, fe(3+) and ferric uptake regulator (fur) regulated operons, pvsabcde and psua-pvuabcde, sharing high similarity with that related to siderophore biosynthesis and transportation locus in v. parahaemolyticus. siderophore biosynthesis or utilization was blocked when pvsa and pvsd of the pvsabcde operon or pvua, pvub and pvue of the psua-pvuabcde operon was single-gene in-frame mutated ... | 2007 | 17593352 |
| pathogenic bacteria associated with oysters (crassostrea brasiliana) and estuarine water along the south coast of brazil. | oysters and estuarine water samples were collected monthly, from june 1998 to march 1999, in the cananéia estuary, on the south coast of são paulo, brazil, and analyzed for bacterial hazards with and without depuration in filtered estuarine water. aeromonas spp., plesiomonas shigelloides, vibrio cholerae o1, vibrio parahaemolyticus, and vibrio vulnificus were counted in oyster samples using the most probable number (mpn) and their presence verified in the surrounding estuarine water samples. the ... | 2007 | 17613090 |
| a targeting approach for delivery of polymer microparticle-antibody conjugate against vibrio parahaemolyticus-induced cytotoxicity to human intestinal epithelial cells. | a major traditional of antibacterial drugs is antibiotic which promotes more rapid release of the toxins from bacteria cells in human body, which causes severe infection. the thermostable direct hemolysin (tdh) has been proposed as a major virulence factor of vibrio parahaemolyticus (vp). this study covers the preparation of polymer microparticle-antibody conjugate for the development of a drug targeting approach for antibacterial drug delivery. the chemical binding of antibodies (ab) to latex b ... | 2007 | 17613661 |
| rapid and specific identification of 5 human pathogenic vibrio species by multiplex polymerase chain reaction targeted to dnaj gene. | a multiplex polymerase chain reaction (pcr) method, specifically designed for application in routine diagnostic laboratories, was developed for identifying 5 human pathogen vibrio species: vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio mimicus, and vibrio alginolyticus. this assay directed toward the dnaj gene was tested on a total of 355 strains representing 13 vibrio species and 17 non-vibrio species. specific pcr fragments were produced in isolates belonging to the 5 targ ... | 2007 | 17614235 |