Publications

definition of subclasses of adenosine receptors associated with adenylate cyclase: interaction of adenosine analogs with inhibitory a1 receptors and stimulatory a2 receptors.	the structure-activity relationships of 63 adenosine analogs as agonists for the a1 adenosine receptors that mediate inhibition of adenylate cyclase activity in rat fat cells and for the a2 adenosine receptors that mediate stimulation of adenylate cyclase in rat pheochromocytoma pc12 cells and human platelets were determined. the lack of correspondence between the structure-activity relationships of these analogs at the a1 and a2 receptors appear definitive in terms of establishing the existence ...	1987	3580959
physiologic and biochemical effects of immobilization on muscle.	muscle strength rapidly declines during limb immobilization because of a decrease in muscle size and a decrease in tension per unit of muscle cross-sectional area. muscle fatigability also increases rapidly after limb immobilization. muscles within limbs fixed by plaster casts have lower levels of resting glycogen and adenosine triphosphate (atp), a more rapid depletion of muscle glycogen and atp during work, a greater increase in lactate during work, and a decreased capacity to oxidize fatty ac ...	1987	3581565
visual and oculomotor function in optic chiasma-sectioned rabbits.	anatomical and physiological findings indicate that the crossed optic fibres of the rabbit have a crucial role in binocular vision. in order to directly examine the visual functions of the uncrossed fibre system, a technique of sectioning the optic chiasma midsagitally was developed. both normal and chiasma-sectioned rabbits were tested on a variety of visual discrimination tasks as well as such oculomotor control functions as the optokinetic and vestibulo-ocular reflexes. following transection ...	1987	3582536
the connections of the peripeduncular area studied by retrograde and anterograde transport in the rat.	lying just ventral to the medial geniculate nucleus and dorsal to the cerebral peduncle, the peripeduncular area (ppa) seems to be implicated in the expression of several sexual reflexes in rodents. the aim of the present investigation was to establish the organization of the afferent and efferent projections of the rat's ppa in an attempt to provide a neural substrate corresponding to the behavioral observations. based on both retrograde and anterograde transport methods, our results suggest th ...	1987	3584548
development of neural receptors for serotonin in the murine bowel.	during the ontogeny of the enteric nervous system, the varicosities of mature neurons contact dividing neural precursors, which persist in the developing murine gut for several weeks postnatally. this phenomenon has led to the hypothesis that the release of transmitter from mature neurons may influence the subsequent development of uncommitted neuroblasts. in order to test this hypothesis, it is necessary to know the timing of the expression of postsynaptic receptors for enteric neurotransmitter ...	1987	3584550
abnormal daily periodicity of serum thyrotropin (tsh) and evidence for defective tsh suppression in a case of non-neoplastic syndrome of inappropriate tsh secretion.	a non-neoplastic syndrome of inappropriate secretion of tsh (itshs) was diagnosed in a hemithyroidectomized and clinically euthyroid 44-yr-old man, who also exhibited limping (perthes' disease), genu valgum, pes supinatus and lateral nystagmus. computed tomography demonstrated an enlarged sella turcica due to empty sella. baseline serum t3, t4, free t3, free t4 and tsh fluctuated between 179 and 274 ng/dl, 6.0 and 13.2 micrograms/dl, 4.2 and 6.0 pg/ml, 7.6 and 15.3 pg/ml, and 4.3 and 33.0 microu ...	1987	3584859
lyme disease in texas.		1987	3591105
lyme disease in texas.		1987	3591105
the growth rate of sensory nerve fibres in the mammalian embryo.	i have used a novel quantitative electron microscopic method to determine the rate at which nerve fibres grow towards their targets during development. the rate of recruitment of nerve fibres to the maxillary nerve of the mouse embryo was determined by counting the number of axon profiles in the nerve sectioned close to its emergence from the trigeminal ganglion at closely staged intervals throughout its early development. the rate of change of fibre number with distance along this nerve was det ...	1987	3652972
expression of liver fluke antigens by mouse cells.		1987	3654047
expression of liver fluke antigens by mouse cells.		1987	3654047
[3-dimensional reentry in paroxysmal ventricular tachycardias: the results of electrophysiological mapping].	the mechanism of sustained ventricular tachycardias with a focal type of activation initiated by an extrastimulus was studied in isolated rabbit heart ventricle preparations by endocardial and epicardial mapping. sixty-four electrograms were simultaneously recorded using unipolar electrodes (32 on each side of the preparation). the electrodes were spaced 3 mm apart. data recording and processing were computerized. sustained (greater than 0.5 min) tachycardias of a focal type were registered in 6 ...	1987	3656897
influence of nifedipine on arterial baroreflex modulation of heart rate control during dynamic increases in arterial pressure: studies in normal man.	studies in animals have demonstrated that calcium channel blocking agents exert important influences on autonomic mechanisms in addition to their direct vascular effects. previous studies in our laboratory showed that clinical doses of nifedipine sensitized baroreceptor-mediated control of peripheral vascular resistance in normal human subjects. however, baroreflex control of vascular tone does not necessarily imply parallel control of heart rate. a series of experiments was therefore performed ...	1987	3661368
[volatile anesthetics and n-alcohols inhibit the uptake of noradrenaline in pheochromocytoma cells].	studies with perfused animal organs did not show a significant inhibition of the norepinephrine uptake by volatile anesthetics however, the uptake of norepinephrine into isolated chromaffin granules proved to be halothane-sensitive , and there is indirect evidence for an inhibition of norepinephrine uptake by halothane in the dog saphenous vein. so far no literature is available on norepinephrine uptake into cultured cells in the presence of volatile anesthetics. the rat pheochromocytoma cell li ...	1987	3661947
vasculitis and renal disease in nail-patella syndrome: case report and literature review.	a 57 year old man with nail-patella syndrome (nps) and associated renal disease is described who developed an inflammatory polyarthropathy and polyarteritis-like vasculitis. vasculitis and serum complement abnormalities have not previously been reported in nps. nps is a rare autosomal dominant connective tissue disorder affecting both mesenchymal and ectodermal tissue. the condition is reviewed with particular reference to its renal pathology, including the distinctive electron microscopic (em) ...	1987	3662646
immediate and long-term effects of opiate antagonists on postictal behaviour following amygdala kindling in the rat.	male wistar rats implanted with bipolar electrodes in the amygdaloid complex were kindled. subcutaneous injection of naloxone or naltrexone in low doses--0.12 and 0.24 mg/kg, respectively--dramatically reduced the postictal behavioural depression (bd) at 10 or 60 min. remarkably, the bd was still reduced one day later. it would appear that the brain mechanisms involved in postictal bd use mu-receptors since bd is quite sensitive to low doses of the preferential antagonists naloxone and naltrexon ...	1987	3666034
liquid chromatographic analysis of alizapride and metoclopramide in human plasma and urine using solid-phase extraction.	a universal rapid, sensitive and selective high-performance liquid chromatographic method with uv detection at 230 nm has been developed for the determination of benzamide drugs in human plasma and urine. sample pretreatment is carried out using solid-phase extraction columns, resulting in very high extraction recoveries of the compounds investigated (alizapride, metoclopramide, alpiropride, amisulpride). the detector response is linear from 25 to 10,000 ng/ml, and the detection limit is 3 ng/ml ...	1987	3667781
infection following a dog bite.		1987	3668208
effect of dietary glycine on methionine metabolism in rats fed a high-methionine diet.	the alleviation mechanism of methionine toxicity by dietary glycine was investigated in weanling rats fed a high-methionine diet. when rats were fed a 10% casein diet containing 2% methionine, the activities of methionine adenosyltransferase, cystathionine beta-synthase, and cystathionine gamma-lyase, which participate in the methionine metabolism in the transsulfuration pathway, were significantly enhanced. but the addition of 2% glycine to the high methionine diet did not cause further increas ...	1987	3668700
properties of equine anti-lipopolysaccharide hyperimmune plasma: binding to lipopolysaccharide and bactericidal activity against gram-negative bacteria.	anti-lipopolysaccharide equine hyperimmune plasma (anti-lps), which has been used successfully to treat lps (endotoxin)-mediated disorders, has been further characterised. igg present in anti-lps had the highest affinity for lps prepared from salmonella typhimurium, s. typhi, s. abortus equi and shigella flexneri and intermediate affinity for escherichia coli o55:b5, e. coli o127:b8 and s. enteritidis. anti-lps destroyed by means of complement activation a wide range of gram-negative bacteria, i ...	1987	3669052
conservative management of muscle-infiltrating bladder cancer: prospective experience.	between may 1979 and july 1983, 217 consecutive patients with documented primary bladder tumors invading muscle were evaluated to determine the fate of patients with conservatively treated muscle-infiltrating bladder cancer. the disease was re-staged by urine cytology, bimanual examination with the patient under anesthesia and transurethral biopsy or resection. of the 217 patients 172 underwent total or partial cystectomy and 45 (21 per cent, 37 with stage t2, 7 with stage t3a and 1 with stage t ...	1987	3669160
conservative management of muscle-infiltrating bladder cancer: prospective experience.	between may 1979 and july 1983, 217 consecutive patients with documented primary bladder tumors invading muscle were evaluated to determine the fate of patients with conservatively treated muscle-infiltrating bladder cancer. the disease was re-staged by urine cytology, bimanual examination with the patient under anesthesia and transurethral biopsy or resection. of the 217 patients 172 underwent total or partial cystectomy and 45 (21 per cent, 37 with stage t2, 7 with stage t3a and 1 with stage t ...	1987	3669160
[role of hippocampal acetylcholine-sensitive neurons in different stages of nictitating membrane conditioning of rabbits].		1987	3672137
[an example of bvd infection from practice].		1987	3672471
[biopharmaceutical evaluation of the rectal administration of mefenamic acid. iv. biological availability of mefenamic acid on the base of polyoxyethylene glycol with the addition of polysorbates].		1987	3673627
measurement of branched-chain ketoacids in plasma by high-performance liquid chromatography.	branched-chain ketoacids were isolated from plasma or serum samples by acidification, passage through a cationic exchange resin, ether extraction, and extraction of the ether layer with phosphate buffer. the recovery of 2-[1-14c]ketoisocaproate taken through these procedures averaged 95 +/- 3%. branched-chain ketoacids were measured by high-performance liquid chromatography using a single mobile phase (sodium phosphate:acetonitrile). in normal human subjects, mean +/- sd fasting levels of 2-keto ...	1987	3674376
sample preparation for gas chromatography with electron capture detection: determination of total and free thyroxin in serum.	relatively clean gas chromatography with electron capture detection (gc-ecd) chromatograms are obtained for both total and free thyroxin (t4) in serum by improving sample preparation. this is based on establishing a sequence of steps that cumulatively overcome two classes of interference: those present in the initial sample and those introduced by the procedure. the main source for the latter contaminants is the derivatization step, a problem that was largely overcome by employing hplc after thi ...	1987	3674391
changes in intraconceptus pressure in the rabbit during days 7-10 of pregnancy.	the present study established the magnitude of and temporal changes in the pressure within in situ rabbit conceptuses during early pregnancy. at the 7th, 8th, 9th, or 10th day post coitum (dpc), animals were anesthetized, and the number and dimensions of implantation sites were recorded. conceptus pressure was measured by the servo-nulling method employing a glass micropipette. the mean number of sites was 6/cornu; spacing appeared normal. implantation dome volume increased from 7 through 10 dpc ...	1987	3593839
phenylphosphonate: a 31p-nmr indicator of extracellular ph and volume in the isolated perfused rabbit bladder.	31p-nmr has been used extensively to estimate intracellular ph. it also can be used to measure extracellular ph and volume when an nmr-detectable extracellular phosphorous probe is used. phosphonic acids have been suggested as useful 31p-nmr extracellular markers. the present study was designed to assess the utility of phenylphosphonic acid (ppa) as a 31p-nmr extracellular marker in perfused smooth muscle. rabbit bladder strips were exposed to ppa concentrations of 1-20 mm. tension development i ...	1987	3594737
storage conditions and stability of thyrotropin and thyroid hormones on filter paper.	we evaluated the effects of temperature and humidity on thyroid hormones (t4, t3) and thyrotropin (tsh) measured in blood spots dried on filter paper. rias for t4 and t3 blood spots were optimized to measure these analytes over the neonatal and euthyroid adult reference intervals. sensitivities of the t3 and t4 assays were 0.5 and 10 nmol/l, respectively. a blood-spot immunoradiometric assay for tsh involving magnetizable particles was developed with a sensitivity of 6 milli-int. units/l. contro ...	1987	3594835
storage conditions and stability of thyrotropin and thyroid hormones on filter paper.	we evaluated the effects of temperature and humidity on thyroid hormones (t4, t3) and thyrotropin (tsh) measured in blood spots dried on filter paper. rias for t4 and t3 blood spots were optimized to measure these analytes over the neonatal and euthyroid adult reference intervals. sensitivities of the t3 and t4 assays were 0.5 and 10 nmol/l, respectively. a blood-spot immunoradiometric assay for tsh involving magnetizable particles was developed with a sensitivity of 6 milli-int. units/l. contro ...	1987	3594835
effect of postmortem factors on muscarinic receptor subtypes in rat brain.	although prior studies have supported the validity of measuring total muscarinic receptor binding in postmortem brain, there has not been a study of postmortem effects on muscarinic receptor subtypes, m1 and m2, defined by high and low affinity for pirenzepine, respectively. we have examined in rat brain the effect of postmortem delay at room temperature, storage at 4 degrees c and -20 degrees c, and multiple freeze/thaw cycles on total muscarinic binding, measured with [3h]quinuclidinylbenzilat ...	1987	3598588
immunizations among hospital personnel.	to minimize the likelihood of transmission of certain infectious diseases within the hospital, the 5 million us health care personnel are becoming a special target group for immunization programs. a review of immunizations conducted by a hospital employee health service demonstrated that 80% of hospital employees are adequately protected against tetanus/diphtheria, and 97% are immune to rubella. in contrast, only 28% of at-risk employees are immunized against hepatitis b and only 2% are immunize ...	1987	3598735
[hypolipidemic action of the racemic methyl ester of 16,16-dimethyl-d-homo-8-isoestrone].	racemic 16,16-dimethyl-d-homo-8-isoestrone methyl ester (substance 1) was shown to decrease the content of cholesterol in blood serum and liver tissue of animals with induced hyperlipidemia (rats, guinea pigs, rabbits) as well as to decrease the rate of atherosclerotic impairments in rabbit aorta. as distinct from usual estrogen, the substance i did not cause hypertriglyceridemia but normalized triglycerides in blood and liver tissue. substance i exhibited low toxic effects.	1987	3604144
[hypolipidemic action of the racemic methyl ester of 16,16-dimethyl-d-homo-8-isoestrone].	racemic 16,16-dimethyl-d-homo-8-isoestrone methyl ester (substance 1) was shown to decrease the content of cholesterol in blood serum and liver tissue of animals with induced hyperlipidemia (rats, guinea pigs, rabbits) as well as to decrease the rate of atherosclerotic impairments in rabbit aorta. as distinct from usual estrogen, the substance i did not cause hypertriglyceridemia but normalized triglycerides in blood and liver tissue. substance i exhibited low toxic effects.	1987	3604144
effect of protein-modifying reagents on water-soluble and membrane bound ovarian lh/hcg receptor.	preincubation of corpora lutea membrane and follicular fluid water-soluble receptor with protein-modifying reagents suggests that sulfhydryl, histidyl and tyrosyl groups are involved in the interaction of hcg with the receptor.	1987	3609166
serological studies of british leptospiral isolates of the sejroe serogroup. iii. the distribution of leptospires of the sejroe serogroup in the british isles.	some 94 strains of leptospires belonging to the sejroe serogroup isolated in the british isles were identified to the serovar level using specific factor sera. seventy strains were identified as leptospira interrogans serovar hardjo, 66 from cattle, 2 from pigs and 1 each from a sheep foetus and a human. twenty-four strains were identified as l. interrogans serovar saxkoebing, most strains were isolated from either wood mice, bank or field voles but strains were also isolated from badgers, a fox ...	1987	3609169
effects of hypercholesterolemia on the microsomal membrane fluidity of intimal-medial versus medial layers of swine aorta: implications for the pathogenesis of vasospasm.	the hypothesis was examined that hypercholesterolemia induces a decrease in arterial microsomal membrane fluidity. to investigate this hypothesis, the fluorescence anisotropy (r) of 1,6-diphenylhexa-1,3,5-triene was measured in aortic microsomes isolated from the intimal-medial (im) and medial (m) layers of swine thoracic aortas. after 10 weeks of feeding a high fat (10% lard) diet, serum cholesterol increased 2.3-fold compared to 3.6-fold in pigs fed a similar diet supplemented with 2% choleste ...	1987	3609245
[effect of piracetam on rat reproductive function and the sexual development of their progeny].	administration of piracetam to infantile rats in a daily dose of 100 mg/kg for 10 days caused acceleration of sexual maturation of females and a slight delay of maturation of males. antenatal administration of the same dose of piracetam exerted a depressive action on the development of the organs of the reproductive system in males from the offspring with a simultaneous increase in the pituitary gonadotropic activity.	1987	3609283
[contractile properties of isolated lung tissue preparations].		1987	3609372
large scale multiplication of bacillus thuringiensis h. 14 asporogenic mutants & b. sphaericus strains for mosquito control.		1987	3610270
thermally induced disulfide bond exchanges in human ige.	the thermally induced changes in human ige was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. when the ige (1 mg/ml) was heated at 56 degrees c for several hours in the absence of sodium dodecyl sulfate, it gave aggregated forms that could be reduced completely to heavy and light chains with dithiothreitol. on the other hand, in the presence of sodium dodecyl sulfate (0.1%), the ige was changed by heating to five components with small mol. wts dependent on temperat ...	1987	3614214
detecting in vitro anthelmintic effects with a micromotility meter.	an in vitro target parasite anthelmintic assay utilizing a micromotility meter has been developed and validated. haemonchus contortus, an economically important ruminant helminth with worldwide distribution, was the parasite used in the model. four commercially available ruminant anthelmintics (albendazole, ivermectin, levamisole hydrochloride and coumaphos) were initially evaluated at concentrations of 200, 150, 100 and 50 micrograms ml-1. all four significantly affected helminth motor activity ...	1987	3617429
differences in the stimulation of repair replication by 3-aminobenzamide in lymphoblastoid cells damaged by methylmethanesulfonate or ultraviolet light.	human lymphoblastoid cells damaged by u.v. light accumulated dna breaks in the presence of cytosine arabinoside and hydroxyurea at a frequency similar to that of cells damaged by methylmethanesulfonate. 3-aminobenzamide (1 mm) reduced the net strand-break frequency detected after either kind of damage. repair replication, however, was stimulated only in methylmethanesulfonate-damaged cells. this stimulation is therefore not related directly to the dna strand-break frequencies and concomitant pol ...	1987	3621467
effect of apoe on triglyceride emulsion interaction with hepatocyte and hepatoma g2 cells.	the uptake and internalization of a triglyceride emulsion by rat hepatocytes in culture less than 24 hr was either inhibited or uninfluenced by apoe. apoe significantly increased the uptake of these emulsions in later cultures. specific low density lipoprotein (ldl) binding was similar for hepatocyte monolayers prior to and after 24 hr. rat hepatocytes in culture for 2 days, which were treated with collagenase, detached and then replated within 1 hr and were apoe-responsive in 2 hr. heparin inhi ...	1987	3625037
protein binding of glycopeptide antibiotics with diverse physical-chemical properties in mouse, rat, and human serum.	in previous studies of the pharmacokinetics and urinary excretion of nine glycopeptides with diverse isoelectric points (pi), as pi decreases, the total systemic and renal clearance, urinary recovery, and volume of distribution decrease, whereas the half-life increases. with glycopeptides of similar pi, clearance decreases and half-life increases with increasing lipophilicity. the present study examines the serum protein binding of these glycopeptide antibiotics in mouse, rat, and human serum an ...	1987	3625479
[proliferative activity of experimental brain tumors].	the method of autoradiography was used to study the parameters of the cell cycle and the intensity of proliferative processes in three transplantable rat brain tumors differing in the duration of the latent period and sensitivity to chemotherapy. the tumors were found to be similar in the duration of the cell cycle and its phases, the mean values of the label index and growth fraction, and were characterized by the irregularity of the proliferative processes within the tumor; the label index was ...	1987	3564792
[mechanisms of tumorigenesis].	in the etiology of human cancers mutational events, selective amplification of specific cellular sequences after treatment of cells with chemical and physical carcinogens, and viruses are discussed. in cancers of the anogenital tract it appears that these as well as possibly additional human cancers are caused by the persistence of specific viral genes and the failure of an intracellular surveillance system for such genes. the modification of host cell genes should primarily result from interact ...	1987	3566871
an examination of ciliary neuronotrophic factors from avian and rodent tissue extracts using a blot and culture technique.	we have previously reported a technique for determining the apparent molecular weight (mr) of ciliary neuronotrophic factors (cntfs) in crude extracts. this method involves sds-polyacrylamide gel electrophoresis of the extract. western blotting and culture of purified ciliary ganglion neurons on the paper containing the blotted lane. neurons will survive only if in direct contact with the trophic factor band and the surviving neurons, when stained with a vital dye, will outline the cntf band the ...	1987	3567656
an examination of ciliary neuronotrophic factors from avian and rodent tissue extracts using a blot and culture technique.	we have previously reported a technique for determining the apparent molecular weight (mr) of ciliary neuronotrophic factors (cntfs) in crude extracts. this method involves sds-polyacrylamide gel electrophoresis of the extract. western blotting and culture of purified ciliary ganglion neurons on the paper containing the blotted lane. neurons will survive only if in direct contact with the trophic factor band and the surviving neurons, when stained with a vital dye, will outline the cntf band the ...	1987	3567656
target cues are not required for the guidance of sympathetic preganglionic axons.	the patterns of preganglionic projections in the avian sympathetic system are highly specific. preganglionic axons arising from the t1 spinal segment, for example, project predominantly in the rostral direction, whereas t4 preganglionic axons project predominantly in the caudal direction. moreover, each ganglion receives projections from a distinct set of spinal cord segments. these patterns are established during embryonic life and are correct from the outset. in this study, i have examined the ...	1987	3567660
target cues are not required for the guidance of sympathetic preganglionic axons.	the patterns of preganglionic projections in the avian sympathetic system are highly specific. preganglionic axons arising from the t1 spinal segment, for example, project predominantly in the rostral direction, whereas t4 preganglionic axons project predominantly in the caudal direction. moreover, each ganglion receives projections from a distinct set of spinal cord segments. these patterns are established during embryonic life and are correct from the outset. in this study, i have examined the ...	1987	3567660
comparisons of time intervals and plasma lh concentrations during the ovulatory cycle of broiler breeder hens maintained under either a 24 h light:dark cycle or continuous light.	the possibility that egg production in broiler breeder hens may be increased by selection for reduced oviposition interval under continuous light was investigated by comparing the pattern of pre-ovulatory releases of plasma luteinising hormone (lh) and the associated ovipositions in the same broiler hens maintained under normal cycles (15.25 h light/d) or continuous light. the lighting conditions had no effect on plasma concentrations of lh before and at the pre-ovulatory lh peak in first, mid-s ...	1987	3567681
comparisons of time intervals and plasma lh concentrations during the ovulatory cycle of broiler breeder hens maintained under either a 24 h light:dark cycle or continuous light.	the possibility that egg production in broiler breeder hens may be increased by selection for reduced oviposition interval under continuous light was investigated by comparing the pattern of pre-ovulatory releases of plasma luteinising hormone (lh) and the associated ovipositions in the same broiler hens maintained under normal cycles (15.25 h light/d) or continuous light. the lighting conditions had no effect on plasma concentrations of lh before and at the pre-ovulatory lh peak in first, mid-s ...	1987	3567681
effects of d,l-propranolol on apomorphine induced stereotyped behavior in rats.	the effects of d,l propranolol on stereotyped behavior induced by apomorphine administration was studied quantitatively. d,l propranolol caused a 2.5 leftward displacement of the control dose-response curve constructed to apomorphine-induced stereotypy; the ed50 for apomorphine was reduced from 1.87 +/- 0.38 to 0.74 +/- 0.17. the potentiation was dependent on both d,l-propranolol and apomorphine doses. results are discussed in the light of a possible interference of the central noradrenergic sys ...	1987	3569842
toxicity of methotrexate in rheumatoid arthritis.	seventy-two patients with rheumatoid arthritis had been treated with pulse weekly oral methotrexate with a mean followup of one year. minor side effects (oral ulcers, transient elevation of liver enzymes, nausea, vomiting) were present in 46 patients (63.8%), whereas major side effects (severe infection, cytopenia, respiratory failure, seizures, gastrointestinal bleeding) were present in 7 (9.7%), 2 of whom died. patients with major side effects had shorter disease duration and increased frequen ...	1987	3572937
hmp-shunt and cholesterol metabolism in experimental models involving normal and preneoplastic liver growth.	previous studies from our laboratories have shown a stimulation of hmp-shunt, cholesterol metabolism and dna synthesis during cell proliferation. in order to understand the co-ordinated regulation of these pathways during cell growth, the above metabolic pathways were studied in: liver regeneration after partial hepatectomy, lead-induced liver hyperplasia, liver cell proliferation induced by insulin in streptozotocin-diabetic rats, liver cell proliferation in fasted rats after refeeding and, hep ...	1987	3554466
shigellosis in the south-western cape of good hope 1968-85.	during the period 1968-85 shigella organisms were isolated from stool specimens of 1562 patients attending tygerberg hospital, situated in the south-western province of the cape of good hope of the republic of south africa. shigella flexneri (72% of patients) was the commonest subgroup identified. sh. sonnei was the second-commonest isolate (20%), with smaller numbers of sh. boydii (5%) and sh. dysenteriae (3%). sh. dysenteriae has not been isolated since 1979. in 1985 30% of isolates were resis ...	1987	3556443
voluntary hyperventilation changes recruitment order of parasternal intercostal motor units.	the order of recruitment of single-motor units in parasternal intercostal muscles during inspiration was studied in normal human subjects during quiet breathing and voluntary hyperventilation. electromyograms were recorded from the second and third intercostal spaces by means of bipolar fine wire electrodes. flow at the mouth, volume, end-expired co2, and rib cage and abdominal anterior-posterior diameters were monitored. single-motor units were identified using criteria of amplitude and shape, ...	1987	3558179
nucleotide sequences and properties of the sites involved in lysogenic insertion of the bacteriophage hp1c1 genome into the haemophilus influenzae chromosome.	bacteriophage hp1c1 lysogenizes its host haemophilus influenzae rd by inserting its genome into the bacterial chromosome. the dna segments corresponding to the integration regions on the phage and host chromosomes and the two junctions formed between phage and host sequences on lysogenic insertion were isolated and propagated in escherichia coli hb101 as hybrid plasmids by using pbr322 as the vector. the nucleotide sequences in the vicinity of the point of recombinational insertion were determin ...	1987	3491821
local and systemic antibody responses to shigella infection in rhesus monkeys.		1987	3549918
[isolation and characteristics of shigella antigens during the course of dysentery].	in 2,436 fecal samples and 1,272 urinary samples taken from 633 patients with dysentery caused by s. flexneri, s. newcastle and s. sonnei, shigella antigens were detected by means of the passive hemagglutination test with antibody diagnostic agents and the antibody neutralization test. the antigen-binding activity of shigellae in urine dynamically increased in the course of dysentery. the comparison of the parallel results of both serological tests made it possible to evaluate the dispersion of ...	1987	3551415
antibacterial activity and mechanism of action of 3'-azido-3'-deoxythymidine (bw a509u).	the thymidine analog 3'-azido-3'-deoxythymidine (bw a509u; azidothymidine [azt]) had potent bactericidal activity against many members of the family enterobacteriaceae, including strains of escherichia coli, salmonella typhimurium, klebsiella pneumoniae, shigella flexneri, and enterobacter aerogenes. azt also had bactericidal activity against vibrio cholerae and the fish pathogen vibrio anguillarum. azt had no activity against pseudomonas aeruginosa, gram-positive bacteria, anaerobic bacteria, m ...	1987	3551832
failure of klebsiella pneumoniae antibodies to cross-react with peripheral blood mononuclear cells from patients with ankylosing spondylitis.	cross-reactivity between antibodies to 2 strains of klebsiella pneumoniae (k43 and f77) and the peripheral blood lymphocytes of patients with ankylosing spondylitis (as) was examined in 3 separate antibody binding and cytotoxicity assays. using k pneumoniae antisera in a chromium release cytotoxicity assay, we found no difference in the reactions of cells from as patients and those from control subjects. this result contrasts with the results of previous studies. similarly, using an enzyme-linke ...	1987	3551965
localization of melanotropin-like peptides in the central nervous system of two insect species, the migratory locust, locusta migratoria, and the fleshfly, sarcophaga bullata.	by use of well characterized antisera in the peroxidase-antiperoxidase method, we were able to demonstrate alpha msh and beta msh immunoreactive cells and nerve fibres within the nervous system of adults and larvae of locusta migratoria and 3-, 5- and 8-day-old adult sarcophaga bullata. in neither of these insect species, any immunoreaction was obtained with a gamma 3msh-antiserum. double immunohistochemical stainings revealed that alpha msh-like and beta msh-like substances are located in diffe ...	1987	3552238
dirofilaria immitis: identification and characterization of circulating parasite antigens.	a method for the identification of circulating parasite antigens in filarial nematode infections was developed using canine infections with dirofilaria immitis as a model. filarial antigens ranging in molecular weight from 211 to 13 kda were extracted from the sera of microfilaremic dogs by a solid phase immunobinding procedure and identified by immunostaining of western blots. a major antigen of 104 kda was selected for further characterization. the 104 kda circulating antigen showed antigenic ...	1987	3552713
congo red binding phenotype is associated with hemin binding and increased infectivity of shigella flexneri in the hela cell model.	wild-type isolates of shigella flexneri bind the dye congo red from solid media, thus producing red (crb+) colonies. mutants which fail to bind the dye produce white colonies (crb-) and are avirulent in a variety of systems. in s. flexneri the ability to bind congo red correlates with the ability to bind hemin and protoporphyrin ix. binding of hemin by crb+ s. flexneri was observed both in solid media and in liquid assays. results of competition experiments suggest that congo red and hemin bind ...	1987	3553000
pathology as it relates to ear surgery. vi. cochlear implantation.	the surgical anatomy and pathology of the cochlea have been reviewed in relation to cochlear implant surgery. animal experimentation, as well as human temporal bone studies, have shown that the implant electrodes were well tolerated by the cochlea. the possible chemical and mechanical trauma induced by the electrodes can be avoided by better choice of shape, size, length and material of the implants. long-term electrical stimulation did not seem to cause any deleterious effects on the neuronal p ...	1987	3553399
local cerebral metabolic effects of l-dopa therapy in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced parkinsonism in monkeys.	the quantitative 2-deoxy[14c]glucose autoradiographic method was used to map the distribution of alterations in local cerebral glucose utilization that accompanies clinically effective chronic l-dopa therapy of rhesus monkeys made parkinsonian by the administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (mptp). this pattern of changes was compared to the effects of a similar treatment regimen in normal monkeys. l-dopa (100 mg with 10 mg carbidopa) was administered orally to normal and p ...	1987	3497401
[experimental testing of the left-ventricular and biventricular assistant systems in dogs].	the left-ventricular (lvad) and bi-ventricular assistant system (bvad) were tested in dogs. our hitherto existing experiences give information specially for the indication of lvad and bvad. the risk of a belated application of the lvad exists in case of only a clinical control by pressure parameters for the degree of a cardiac insufficiency caused by coronary starvations. an aortic pressure of nearly 150/70 mm hg could be reached by means bvad on condition of heart fibrillation and a left-atrial ...	1987	3497493
expression of the c-myc proto-oncogene in growth stimulated porcine thyroid follicle cells.	in the present investigation the expression of c-myc in porcine thyroid follicle cells following the incubation with tsh or egf was studied. egf did not only have a mitotic effect on thyroid follicles but also increased c-myc mrna. the increase in c-myc rna is an early event in egf stimulated thyroid cells. tsh had no effect on c-myc rna levels of porcine thyroid cells. egf-binding and sensitization to the mitotic activity of egf, however, is increased by tsh. while tsh stimulates camp productio ...	1987	3497508
difference in monoamine oxidase b activity between c57 black and albino nmri mouse strains may explain differential effects of the neurotoxin mptp.	monoamine oxidase b (mao-b) is the key enzyme in the conversion of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(mptp) to n-methyl-4-phenyl-pyridinium ion (mpp+) which causes degeneration of dopaminergic nigral neurons. using a histochemical tetrazolium method for mao-b with tyramine as substrate and chlorgyline for the inhibition of mao-a, black c57 mice were found to have a higher brain mao-b activity than similar aged albino nmri mice. the difference, which was in general density rather than d ...	1987	3498133
further evidence that there is more than one adrenal 21-hydroxylase system.	the 21-hydroxylase activity of microsomes isolated from bovine adrenal cortex have been assayed using [21-3h]17-hydroxypregnenolone and [1,2-3h]17-hydroxyprogesterone as substrates. when the assays are performed in the presence of an nadh regenerating system, to inhibit steroid 3 beta-hydroxy isomerase-dehydrogenase activity, the microsomes oxidize the 3 beta-hydroxy-5-ene steroid at a rate of 0.37 nmol/min.nmol cytochrome p-450 and the 3-keto-4-ene steroid at a rate of 6.4 nmol/min.nmol. when t ...	1987	3501041
the prevalence of chlamydia trachomatis in a young, sexually-active population.	a prospective study was carried out to determine the prevalence of chlamydia trachomatis among 1000 sexually-active women at the family planning association clinic in melbourne. this organism was isolated from the cervices of 5.1% of screened women. the women were surveyed about their sexual and gynaecological history, and symptoms of discharge or pain. it was found that women who gave positive results for the presence of chlamydia were younger, and had commenced intercourse at an earlier age. r ...	1987	3501060
detection of colonies of bacteroides gingivalis by a rapid fluorescence assay for trypsin-like activity.		1987	3507625
neutral endopeptidase-24.11 (enkephalinase). biosynthesis and localization in human fibroblasts.	the biosynthesis, glycosylation and subcellular localization of the neutral endopeptidase-24.11 were studied in cultured human fibroblasts. the enzyme was synthesized as a precursor (mr 88,000) containing four or five n-linked oligosaccharides. within 1 h the synthesis-mature (mr 94,000) endopeptidase-24.11 was formed and contained sialylated oligosaccharides. the half-life of endopeptidase-24.11 was 3.7 days and in the presence of 10 mm-nh4cl it increased to 6 days. mature endopeptidase-24.11 w ...	1987	3481263
actions of prostaglandins e2 and f2 alpha on release of 14c-labelled mucins from rat submandibular salivary acini in vitro.	mucin secretion was studied in vitro using well-characterized preparations of isolated acini. pge2 significantly (p less than 0.05) increased release of [14c]-glucosamine labelled mucins at the highest concentration tested (10(-5) m), but was ineffective at lower doses (10(-9)-10(-6) m). pgf2 alpha had no effect on mucin secretion over this concentration range. pge2 (10(-9)-10(-5) m) did not modify isoproterenol stimulated mucin secretion. the cyclooxygenase inhibitor indomethacin (10(-7)-10(-5) ...	1987	3482153
[comparison of different campylobacter-selective media in the study of feces samples of domestic animals].		1987	3426537
neutron scattering determination of the binding of prothrombin to lipid vesicles.	low-angle neutron scattering is used to study the binding of human prothrombin to small single-bilayer vesicles consisting of phosphatidylcholine and phosphatidylserine (1/1 w/w). the radius of gyration of prothrombin indicates that it is an elongated molecule. the vesicles alone were not observed to coalesce, and their molecular weight, outer radius, and average surface area per lipid were respectively (1.6 +/- 0.32) x 10(6), 114 +/- 4 a, and 110 +/- 18 a2. these values were independent of the ...	1987	3427105
modulation of the defective natural killer activity seen in thalassaemia major with desferrioxamine and alpha-interferon.	we previously observed that natural killer (nk) activity toward k562 cells is markedly depressed in patients with beta-thalassaemia major. here we report that these patients also exhibit significantly decreased (p less than 0.005) nk cytotoxicity against human fibroblasts infected with herpes simplex virus-type 1 (hsv-1) and that the amount of alpha-interferon (alpha-ifn) generated during the latter assays is significantly less than normal (p less than 0.005). this decreased production of alpha- ...	1987	3427826
delivery of theophylline into excised human skin from alkanoic acid solutions: a "push-pull" mechanism.	human skin samples are permeable to theophylline delivered from 1.5% solutions in various alkanecarboxylic acids and their mixtures. the respective permeability coefficients of theophylline, calculated from steady-state flux, correlate negatively with the permeability coefficients of the donor carboxylic acids and positively with the excess free energy of theophylline in the donor phase, or "push" effect. an exception is propionic acid which enhances the penetration of theophylline by promoting ...	1987	3430340
localization of source of immunosuppressive factor in the human male reproductive tract.	the source of the immunosuppressive factor (isf) in the human male reproductive tract which suppresses various in vitro immune responses has been identified. we describe here the prostatic localization of this factor, based on investigating the fractions of split ejaculates, ejaculates of individuals with blocked epididymis and the secretions of the prostate obtained by prostatic massage.	1987	3430481
[a new measurement of pulmonary venous compliance by r-synchronized volume loading].		1987	3432757
heterologous protection in murine cutaneous leishmaniasis.	mice immunized with a glycolipid antigen (gl) of leishmania major plus adjuvant are relatively resistant to subsequent infection with this protozoan parasite. the gl is affinity purified on the monoclonal antibody wic-79.3 which is l. major-specific and does not react with l. donovani. when another monoclonal, wic-108.3, which cross-reacts with several leishmania species, is used to affinity purify gl from l. donovani, the eluted material can partially protect genetically resistant mice against ...	1987	3436638
physiological and non-physiological forms of iron affect differently proliferation and ferritin synthesis in human mononuclear cells in vitro.	the effect of iron in different forms on the proliferative response to mitogen and ferritin content of human mononuclear leukocytes (mnl) was studied in vitro. iron-loaded transferrin (fetf) but not apotransferrin (apotf) significantly enhanced the mitogenic response of mnl in serum-free culture. removal of tf from serum markedly reduced its effectiveness as a serum supplement. this effect could be almost totally reversed when the serum was reconstituted with both iron and apotf. four forms of i ...	1987	3441921
lipid a and related compounds. xiv. a new synthesis of lipid y, the reducing sugar moiety of salmonella- and proteus-type lipid a.		1987	3442881
[pharmacological studies on y-8894. (vii). effects on transient cerebral ischemia-induced amnesia in rats].	the effects of y-8894 on experimental amnesia in rats induced by transient cerebral ischemia (600 sec) according to the method of pulsinelli and brierley were studied using the one trial passive avoidance response and the pole climbing discrete avoidance response. all drugs were administered to the rats immediately after recirculation. the following results were obtained: 1) in the one trial passive avoidance response test, y-8894 (2.5, 5 and 10 mg/kg, i.p.) improved significantly the decreased ...	1987	3443414
genetic parameter estimates for preweaning growth traits in santa gertrudis cattle.	genetic parameters were estimated for birth weight and weaning weight from records collected on 1,894 santa gertrudis calves (939 bulls, 955 heifers) during the 8-yr period, 1978 through 1985. variance and covariance components were estimated separately by sex and combined across sexes utilizing mixed-model, least-squares procedures (henderson's method 3). the mathematical model assumed for estimating variance and covariance components by sex included effects of year, sire-within-year and age of ...	1987	3443570
serological investigations on the cause of diarrhoea on the cape verde islands.	in the serological tests 45.9% of all samples reacted positive against rota virus. 20.8% of samples revealed entamoeba histolytica positive sera, 25% shigella flexneri positive sera, 12.3% shigella sonnei and 13% shigella dysenteriae positive sera. campylobacter jejuni antibodies were found in 19% of samples, whereas 6.3% of sera reacted positive against yersinia enterocolitica type 3 and 4.8% against type 9.	1987	3443746
serological investigations for the cause of diarrhoea among patients and personnel in the minna and abeokuta hospitals/nigeria.	188 blood samples were investigated for antibodies against bacteria causing diarrhoea in two hospitals in minna and abeokuta in nigeria. antibodies were found against entamoeba histolytica, yersinia enterocolitica, campylobacter jejuni, salmonella typhi, salmonella paratyphi c, shigella dysenteriae, shigella sonnei, shigella boydii, shigella flexneri and rotavirus.	1987	3443752
moniliella suaveolens var. nigra.		1987	3444445
some biological properties of outer membrane proteins of shigella.	outer membrane proteins (omp) derived from two antigenically different representatives of shigella (sh. flexneri 3a and sh. sonnei phase i) were tested for the toxicity, pyrogenicity, ability to induce shwartzman reaction as well as for their influence on the leukocyte system. ld50 dose determined on mice was 28 mg/kg for omp of sh. flexneri and 23 mg/kg for omp of sh. sonnei. both preparations injected intravenously to rabbits caused moderate increase of body temperature, expressed by the value ...	1987	3334007
secretory activity and endocrine regulation of male accessory glands in the blood-sucking bug panstrongylus megistus (hemiptera: reduviidae).	the epithelial cells of panstrongylus megistus male accessory glands (mag) present ultrastructural characteristics of a secretory cell. their secretory products are accumulated in the lumen of the four mag lobes. during the first 8 days of adult life a strong secretion activity occurs, accumulating enough material to produce the first spermatophore. cerebral neurosecretions as well as juvenile hormone are both involved in mag secretory activity regulation. juvenile hormone seems to be the respon ...	1987	3334404
interaction of the formed elements of blood with the coronary vasculature in vivo.	considerable attention is being given to the interactions that occur among blood platelets, neutrophils, and the vascular endothelium. there is an increasing awareness that the various blood elements interact in the process of thrombus formation and vascular occlusion. in addition, interactions among these cells can lead to the formation and release of vasoactive substances that have the potential to modulate regional blood flow. this review focuses on the coronary vascular bed and an assessment ...	1987	3542578
expression of myosin heavy chain isoforms in regenerating myotubes of innervated and denervated chicken pectoral muscle.	monoclonal antibodies were prepared to stage-specific chicken pectoral muscle myosin heavy chain isoforms. from comparison of serial sections reacted with these antibodies, the myosin heavy chain isoform composition of individual myofibers was determined in denervated pectoral muscle and in regenerating myotubes that developed following cold injury of normal and denervated muscle. it was found that the neonatal myosin heavy chain reappeared in most myofibers following denervation of the pectoral ...	1987	3542634
cloning of genes from members of the family enterobacteriaceae with mini-mu bacteriophage containing plasmid replicons.	an in vivo cloning system that uses derivatives of the escherichia coli bacteriophage mu with plasmid replicons has been extended to five different species of the family enterobacteriaceae. mu and these mini-mu replicon elements were introduced into strains of e. coli, shigella flexneri, salmonella typhimurium, citrobacter freundii, and proteus mirabilis by infection, by transformation, or by conjugation with newly constructed broad-host-range plasmids containing insertions of these elements. ly ...	1987	3542967
phosphorylation, glycosylation, and proteolytic activity of the 52-kd estrogen-induced protein secreted by mcf7 cells.	we have studied the posttranslational modifications of the 52-kd protein, an estrogen-regulated autocrine mitogen secreted by several human breast cancer cells in culture (westley, b., and h. rochefort, 1980, cell, 20:353-362). the secreted 52-kd protein was found to be phosphorylated mostly (94%) on high-mannose n-linked oligosaccharide chains, and mannose-6-phosphate signals were identified. the phosphate signal was totally removed by alkaline phosphatase hydrolysis. the secreted 52-kd protein ...	1987	3543022
phosphorylation, glycosylation, and proteolytic activity of the 52-kd estrogen-induced protein secreted by mcf7 cells.	we have studied the posttranslational modifications of the 52-kd protein, an estrogen-regulated autocrine mitogen secreted by several human breast cancer cells in culture (westley, b., and h. rochefort, 1980, cell, 20:353-362). the secreted 52-kd protein was found to be phosphorylated mostly (94%) on high-mannose n-linked oligosaccharide chains, and mannose-6-phosphate signals were identified. the phosphate signal was totally removed by alkaline phosphatase hydrolysis. the secreted 52-kd protein ...	1987	3543022
shigellosis in bangladesh.		1987	3543150
a cytosolic protein contains a cryptic mitochondrial targeting signal.	cytosolic dihydrofolate reductase from mouse contains a cryptic mitochondrial targeting sequence. if this sequence is attached to the amino terminus of 'passenger' proteins which by themselves cannot enter mitochondria, the resulting fusion proteins are transported into yeast mitochondria.	1987	3543689