Publications

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Chemical composition and biological activity of the volatile extracts of Achillea millefolium.In this study, flowering aerial parts of wild Achillea millefolium growing on the Mediterranean coast (Sardinia Island, Italy) and on the Atlantic coast (Portugal- Serra de Montemuro) were used as a matrix for supercritical extraction of volatile oil with CO2 (SFE). The collected extracts were analyzed by GC-FID and GC-MS methods and their composition were compared with that of the essential oil isolated by hydrodistillation. A strong chemical variability in essential oils depending on the origi ...201122164800
[superficial mycoses in the city of valparaiso, chile: period 2007-2009].superficial mycoses are infections of the keratinous tissue caused by dermatophytes, yeasts and environmental fungi.201122051613
[mycological and epidemiological aspects of tinea capitis in the sousse region of tunisia].tinea capitis continues to be considered a public health problem in tunisia. the purpose of our study was to investigate trends in the incidence and the mycological and epidemiological aspects of tinea capitis in the sousse region (central tunisia).201121893228
Broad spectrum antidermatophytic drug for the control of tinea infection in human beings.During antifungal evaluation of various plant extracts, free and bound flavonoids of Piper betle were found to be most effective as an antidermatophytic against human pathogenic dermatophytes Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum gypseum and Candida albicans. Dermatophytic fungi cause both superficial and internal mycoses. These mycoses, although normally not lethal, are unpleasant and difficult to cure and cause considerable financial losses. Earlier workers prove that a ...201121929713
tinea faciei due to microsporum canis in children: a survey of 46 cases in the district of cagliari (italy).dermatophytoses are frequent in children, but involvement of the facial skin has peculiar aspects that should be considered a separate entity: tinea faciei. microsporum canis infection in tinea faciei has not been widely documented. to review cases of tinea faciei due to m. canis in children diagnosed at the dermatology clinic, university of cagliari. between 1990 and 2009, all children with dermatophyte infections of the facial skin were recruited for the study after parental consent. diagnosis ...201122011084
identification of novel secreted proteases during extracellular proteolysis by dermatophytes at acidic ph.the dermatophytes are a group of closely related fungi which are responsible for the great majority of superficial mycoses in humans and animals. among various potential virulence factors, their secreted proteolytic activity attracts a lot of attention. most dermatophyte-secreted proteases which have so far been isolated in vitro are neutral or alkaline enzymes. however, inspection of the recently decoded dermatophyte genomes revealed many other hypothetical secreted proteases, in particular aci ...201121919205
analysis of the differentially expressed genes in microsporum canis in inducing smooth skin and scalp tissue conditions.microsporum canis is a common zoophilic dermatophyte, which causes a range of infections. to explore the pathogenic mechanism of tinea capitis, we used the suppression subtractive hybridization (ssh) technique to investigate the differences in gene expression between different cultures of microsporum canis incubated on three different types of mineral media containing child glabrous skin, child scalp tissue and adult scalp tissue. using dot-blot hybridization and real-time pcr technique, we succ ...201121883399
Antifungal activity and chemical composition of essential oils from Smyrnium olusatrum L. (Apiaceae) from Italy and Portugal.The essential oils and supercritical CO(2) extracts of wild Smyrnium olusatrum L. growing in Sardinia (Italy) and in Portugal were investigated. For the study, oils were isolated from total plant aerial part (umbels containing seeds). The content of ß-phellandrene (67.3% vs. 42.7%) and a-pinene (31.9% vs. 1.2%), respectively, the main components of Portuguese and Italian essential oils, declined during the maturation stage of the umbels. Contrarily, some other important components, particularly ...201121902563
Assessment of antimicrobial, insecticidal and genotoxic effects of Melia azedarach L. (chinaberry) naturalized in Anatolia.In this study, antibacterial, antifungal, insecticidal and genotoxic activities of the fruit and leaf extracts of Melia azedarach of Turkish origin were evaluated for the first time. The antimicrobial activity was assessed against Gram (+) and ( - ) bacteria, four Candida species and three dermatophytic fungus (Trichophyton rubrum, Epidermophyton floccosum and Microsporum gypseum). The insecticidal activity of the methanolic fruit extract was performed against the larvae of Aedes aegpyti, Culex ...201122149768
Decreasing Prevalence of Microsporum canis Infection in Korea: Through Analysis of 944 Cases (1993-2009) and Review of Our Previous Data (1975-1992).Since 1957, Microsporum (M.) canis has been one of the common causative agents of dermatophytosis in Korea. We analyzed 944 patients infected with M. canis who attended outpatient clinic over a 17-year period (1993-2009). M. canis infections were diagnosed by mycological examinations, including direct microscopic examinations with 15% KOH and cultures on potato dextrose agar complemented with 0.5% chloramphenicol. Mycological examinations confirmed 164,903 cases of dermatophytosis, 944 (0.6%) of ...201122187358
design, synthesis and antifungal activity of some new imidazole and triazole derivatives.triazole and imidazole are incorporated into the structures of many antifungal compounds. in this study a novel series of 1,2,4-triazole, imidazole, benzoimidazole, and benzotriazole derivatives was designed as inhibitors of cytochrome p450 14α-demethylase (14dm). these structures were docked into the active site of mt-cyp51, using autodock program. sixteen compounds with the best binding energy were synthesized. the chemical structures of the new compounds were confirmed by elemental and spectr ...201121984016
Identification of rare macroconidia-producing dermatophytic fungi by real-time PCR.To understand the pathogenicity and clinical significance of dermatophytes (also known as ringworms), the correct identification of these molds is essential. However, in routine practice they are notoriously difficult to classify and identify. The morphology of macroconidia, which are abundantly produced under suitable in vitro conditions, have provided useful criteria for the identification of many of the dermatophytes. However, several of them, including Microsporum audouinii, M. ferrugineum, ...201121954954
microsporum praecox: molecular identification of a new case and review of the literature.we report a rare case of dermatophytosis due to microsporum praecox in a 28-year-old female horse rider. the skin lesion was located on the right external malleolus. microscopic examination of skin scrapings revealed a dermatophyte which was also isolated in culture. the identification of m. praecox was confirmed by molecular biology (sequence analysis of pcr products amplified from internal transcribed spacer regions with universal primers). combined antifungal therapy with oral terbinafine and ...201120658321
a case of kerion celsi caused by microsporum gypseum in a boy following dermatoplasty for a scalp wound from a road accident.we report a rare case of kerion celsi of the scalp caused by microsporum gypseum in a boy 1 month after he received dermatoplasty for a scalp injury from a road accident. species identification was performed by observation of morphologic and biochemical characteristics and sequencing of the internal transcribed spacers (its) 1 and 2. treatment with oral terbinafine and topical naftifine-ketoconazole cream after washing the hair with ketoconazole shampoo was effective.201120662636
use of itraconazole and either lime sulphur or malaseb concentrate rinse ® to treat shelter cats naturally infected with microsporum canis: an open field trial.in an open non-randomized study, 90 cats with severe dermatophytosis were treated with 21 days of oral itraconazole at 10 mg/kg and one of three topical antifungal rinses applied twice weekly: lime sulphur (lso); reformulated lime sulphur with an odour-masking agent (lsr); or a 0.2% miconazole nitrate and 0.2% chlorhexidine gluconate rinse (mc). weekly examinations and fungal cultures were used to monitor the cats' response to therapy. if at day 42 of treatment cats were still strongly fungal cu ...201120604908
changes of dermatophytoses in southwestern greece: an 18-year survey.the isolation and distribution rate of dermatophytes as causative agents of superficial mycoses of skin, hair, and nails during an 18-year period (1991-2008) at a university hospital are presented. a comparative analysis of epidemiological differences within the first (1991-1999) and the second 9-year period (2000-2008) was performed. skin scrapings, nail, and hair specimens were examined by a direct microscopic examination and culture. identification of dermatophyte species was based on macrosc ...201121331751
meta-analysis of randomized, controlled trials comparing griseofulvin and terbinafine in the treatment of tinea capitis.griseofulvin has been the standard treatment for tinea capitis but newer antifungal agents, particularly terbinafine, are increasingly being used because of their shorter duration of treatment and more consistent absorption rates.201121334096
multilocus differentiation of the related dermatophytes microsporum canis, microsporum ferrugineum and microsporum audouinii.microsporum ferrugineum, an uncommon causative agent of dermatophytosis, has restricted endemicity. iranian strains suspected to be m. ferrugineum from two patients with tinea were analysed using the rdna internal transcribed spacer (its) region and the partial β-tubulin (bt2) and translation elongation factor 1-α (tef1) genes. strains were compared to reference strains to differentiate m. ferrugineum from its relatives microsporum canis and microsporum audouinii. inter-species differences for t ...201221903828
two pediatric cases of microsporum persicolor infection. 201222250631
antifungal property of quaternized chitosan and its derivatives.five water-soluble chitosan derivatives were carried out by quaternizing either iodomethane or n-(3-chloro-2-hydroxypropyl) trimethylammonium chloride (quat188) as a quaternizing agent under basic condition. the degree of quaternization (dq) ranged between 28±2% and 90±2%. the antifungal activity was evaluated by using disc diffusion method, minimum inhibitory concentration (mic) and minimum fungicidal concentration (mfc) methods against trichophyton rubrum (t. rubrum), trichophyton mentagrophyt ...201222100980
Enzymatic activity of Microsporum canis and Trichophyton mentagrophytes from breeding rabbits with and without skin lesions.Microsporum canis and Trichophyton mentagrophytes are zoophilic dermatophytes which can cause skin infections in animals and humans. The clinical expression of this infection strongly varies depending on host, fungal species as well as enzyme production. No comparative studies are available on the enzymatic activities of M. canis and T. mentagrophytes isolated from breeding rabbits. Thus, the aim of this work was to assess the capability of M. canis and T. mentagrophytes isolated from rabbits bo ...201222175244
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