Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| fatal lymphohistiocytic proliferation owing to epstein-barr virus. | 1982 | 6278168 | |
| human lymphoma-lymphoma hybrids and lymphoma-leukemia hybrids. i. isolation, characterization, cell surface markers, and b-cell markers. | four new somatic cell hybrids were obtained by fusion of various burkitt's lymphoma (bl)-derived cell lines that had different selective markers: raji-p3hr-1, daudi-raji, and a p3hr-1-p3hr-1 "autohybrid" derived from two p3hr-1 sublines. in addition, a hybrid was obtained between the daudi (bl) line and the human leukemia cell line k562. the hybrids were extensively characterized by means of chromosome, isozyme, and hla surface markers. the phenotypic differences between the parent cell lines al ... | 1982 | 6278187 |
| human lymphoma-lymphoma hybrids and lymphoma-leukemia hybrids. ii. epstein-barr virus induction patterns. | in addition to the spontaneous expression of markers of the early and late phases of the viral cycle [epstein-barr virus (ebv), early viral cycle antigens (ea), and viral capsid antigen (vca)] by a panel of hybrid cell lines derived by fusion of human hematopoietic cell lines, the induction of these markers by three chemical inducers [5-iodo-2'-deoxyuridine, the tumor promoter 12-o-tetradecanoylphorbol 13-acetate (tpa), and sodium butyrate] were analyzed. a variant of the prototype producer of ... | 1982 | 6278188 |
| tumor promoter 12-o-tetradecanoylphorbol 13-acetate: effect on complement and epstein-barr virus receptors in human lymphoblastoid cell lines. | the effect of the tumor promoter 12-o-tetradecanoylphorbol 13-acetate (tpa) on the c3 and epstein-barr virus (ebv) receptors in various human lymphoblastoid cells was investigated with the use of the erythrocyte-antibody-complement (eac) rosette formation method and a quantitative bioassay for ebv receptors. tpa caused a significant decrease of c3 receptors in the cultures of both raji and sb4 cells (by approximately 50% of the c3 receptors in untreated cultures at 10 ng/ml). kinetic studies rev ... | 1982 | 6278193 |
| selective inhibition of immunoregulatory cell functions by cyclosporin a. | 1982 | 6278023 | |
| generation in vitro of hla-restricted eb virus-specific cytotoxic human t cells by autologous lymphoblastoid cell lines: the role of previous eb virus infection and foetal calf serum. | 1982 | 6277802 | |
| detection of epstein-barr viral dna internal repeats in the nasopharyngeal mucosa of chinese with iga/ebv-specific antibodies. | fifty-six south chinese individuals exhibiting iga antibodies to ebv for 18 months and presenting nasopharyngeal abnormalities were biopsied. four nasopharyngeal carcinomas, two at a very early stage, were detected. in 14 further individuals, without clinical or histopathological evidence of tumor, ebv/dna internal repeats and/or ebna were detected in the biopsied mucosae. the presence of iga/ebv antibodies and/or ebv markers in the nasopharyngeal mucosa may characterize pre-cancerous conditions ... | 1982 | 6277806 |
| natural and antibody-dependent cell-mediate cytotoxicity to cultured target cells superinfected with epstein-barr virus. | the relationship between natural cell-mediated cytotoxicity (ncmc) and antibody-dependent cell-mediated cytotoxicity (adcc) was examined in an epstein-barr virus-infected target cell system. the total adcc reactivity to epstein-barr virus-infected target cells varied considerably with different effector cells, indicating contributions to specificity by the effector cells as well as by antibodies in the sera. to investigate the role of each reactant, the effector cells, sera, and target cells wer ... | 1982 | 6277480 |
| establishment and characterization of an epstein-barr virus-negative lymphoma b-cell from a patient with a diffuse large cell lymphoma. | cell line (lnpl) was established from a tumor biopsy of a nasopharyngeal "histiocytic" lymphoma composed of large noncleaved b-cells. lnpl expressed immunoglobulin m kappa surface immunoglobulin, as did the original tumor. lnpl also expressed ia antigen but lacked epstein-barr virus nuclear antigen. it exhibited a translocation between chromosomes 8 and 14 which has been described in certain other malignant lymphoproliferative disorders. an autologous t-cell line lacked the translocation between ... | 1982 | 6277483 |
| epstein-barr virus (ebv) receptor implantation onto human b lymphocytes changes immunoglobulin secretion patterns induced by ebv infection. | mosaic membrane vesicles containing both epstein-barr virus (ebv) receptors and sendai virus envelope proteins were allowed to form by the previously described membrane solubilization and co-reconstitution technique. the vesicles were allowed to fuse with the membranes of normal human b lymphocytes, whereafter the cells were infected with transforming ebv (b95-8 substrain). compared to similarly infected but otherwise unmanipulated cells, the receptor-implanted lymphocytes responded with a large ... | 1982 | 6277643 |
| human cancers and human viruses. | 1982 | 6277420 | |
| b cell, helper t cell, and suppressor t cell abnormalities contribute to disordered immunoglobulin synthesis in patients following bone marrow transplantation. | the pathogenic mechanisms responsible for the hypogammaglobulinemia that occurs in patients who have undergone bone marrow transplantation were studied using peripheral blood lymphocytes in an in vitro immunoglobulin biosynthesis assay. none of the nine marrow recipients between 2 and 15 months after transplantation produced normal amounts of igg, iga, or igm in response to pokeweed mitogen (a t cell-dependent activator). however, three of these same patients responded to epstein-barr virus (a h ... | 1982 | 6277055 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 9-1982. atypical white cells in the blood of an elderly woman. | 1982 | 6276749 | |
| new epstein-barr virus variants from cellular subclones of p3j-hr-1 burkitt lymphoma. | 1982 | 6276755 | |
| the immunology of cancer of the head and neck with particular reference to oral cancer. | 1982 | 6276835 | |
| [reactivation of epstein-barr virus during primary cytomegalovirus infection in the healthy adult]. | 1982 | 6276860 | |
| dna and protein synthesis in normal and transformed lymphocytes exposed to abrin. | the dose dependent effects of abrin, a toxic d-galactose binding plant lectin, on 3h-tdr and 14c-leucine uptake are studied in normal and epstein barr virus (ebv) transformed lymphocyte cultures. results show that while abrin is highly toxic to both the dna and protein synthesis in ebv lymphocytes, some toxicity to the normal cells is also seen. it is postulated that lymphocyte dna synthesis is affected by ribosomal shutdown induced by the abrin. | 1982 | 6276906 |
| activation of suppressor t cells by autologous lymphoblastoid cells: a mechanism for feedback regulation of immunoglobulin synthesis. | 1982 | 6276467 | |
| virus-associated hemophagocytic syndrome or malignant histiocytosis? | 1982 | 6276521 | |
| a quantitative ultrastructural study of peripheral blood lymphocytes containing parallel tubular arrays in epstein-barr virus and cytomegalovirus mononucleosis. | in the normal peripheral circulation there exists a subpopulation of lymphocytes that is ultrastructurally distinct. this lymphocyte is identified with the electron microscope by the presence of cytoplasmic microtubulelike inclusions called parallel tubular arrays (ptas) and contains fc-receptors for cytophilic antibody. in this study, lymphocytes containing ptas (pta-lymphocytes) were quantitated from serial peripheral blood specimens obtained from two patients with epstein-barr virus (ebv) mon ... | 1982 | 6275709 |
| immunoglobulin expression by human b lymphocytes clonally transformed by epstein barr virus. | epstein barr virus (ebv) immortalizes human b lymphocytes, resulting in long-term lymphoblastoid cell lines. to date, such cell lines have typically been derived by suspension culture of mixed mononuclear cells, infected in vivo or in vitro with ebv. we have derived 208 lymphoblastoid lines by clonal transformation of peripheral blood lymphocytes from 3 neonates, 3 normal adults, and 1 patient with systemic lupus erythematosus (sle), utilizing colony formation in agarose and then separate propag ... | 1982 | 6274955 |
| establishment of human t lymphocyte clones highly cytotoxic for an ebv-transformed b cell line in serum-free medium: isolation of clones that differ in phenotype and specificity. | 1982 | 6274958 | |
| t cell-mediated immunoregulation of epstein barr virus- (ebv) induced b lymphocyte activation in ebv-seropositive and ebv-seronegative individuals. | infection with epstein barr virus (ebv) is accompanied by seroconversion and life-long persistence of the virus in b lymphocytes. during acute ebv-induced infectious mononucleosis, suppressor t cells become activated, which may provide an additional mechanism of host defense against the causative agent. when cultures of lymphocytes from normal adults seropositive for ebv were stimulated with the b95-8 strain of ebv, purified b cells produced increasingly higher numbers of immunoglobulin- (ig) se ... | 1982 | 6274959 |
| b lymphoblast antigen (bb-1) expressed on epstein-barr virus-activated b cell blasts, b lymphoblastoid cell lines, and burkitt's lymphomas. | two new cell surface antigens expressed on b lymphoblastoid cell lines (b-lcl) were defined with cytotoxic mouse monoclonal antibodies. one marker, bb-1 (for b lymphoblast antigen-1), was detected on human and nonhuman primate b-lcl, epstein-barr virus (ebv)-activated b cell blasts, most burkitt's lymphomas, and ia+ b lymphoblast-like myelomas. polyclonal b cell activators such as pokeweed mitogen (pwm) and lipopolysaccharide (lps) also induced the expression of bb-1 on immunoglobulin (ig)-posit ... | 1982 | 6274961 |
| abnormal anti-epstein barr virus antibodies in carriers of the x-linked lymphoproliferative syndrome and in females at risk. | the asymptomatic hemizygous female carriers of the x-linked lymphoproliferative syndrome (xlp) have abnormal antibody responses to ebv. this suggests partial expression of the defect that leads to ebv-provoked life-threatening diseases in their affected sons. ebv specific antibodies were measured in 65 serum samples of 12 obligate carrier females and seven of their daughters (females at risk) during periods ranging from 1 to 5 yr. abnormal qualitative antiviral capsid antigen (vca) igg titers we ... | 1982 | 6274962 |
| persistent infection with adenovirus types 5 and 6 in lymphoid cells from humans and woolly monkeys. | chronic infection with adenovirus types 5 and 6 was established in primary mononuclear leukocytes from human umbilical cord blood and in epstein-barr virus (ebv)-transformed b lymphocytes from human umbilical cord blood and from woolly monkey blood. adenovirus could be recovered from cultures of primary leukocytes and of ebv-transformed lymphocytes for two and three months, respectively, without visible alteration of cell growth. infection in cultures of ebv-transformed lymphocytes from woolly m ... | 1982 | 6274968 |
| ebv molecular epidemiology: lack of strain role in disease manifestation. | 1982 | 6273728 | |
| previous epstein-barr virus infections in children with special educational needs. | 1982 | 6309975 | |
| observations of the aetiology of primary intestinal lymphoma. | 1982 | 6309979 | |
| cell-mediated immunity against epstein-barr virus infected b lymphocytes. | 1982 | 6314570 | |
| detection of antibodies to epstein-barr virus capsid antigen by immune adherence hemagglutination. | the immune adherence hemagglutination assay was found to be as sensitive and specific as the indirect immunofluorescence technique for titration of antibodies to epstein-barr virus capsid antigen. satisfactory virus capsid antigen-specific and negative control antigens for the immune adherence hemagglutination assay were prepared from cell extracts of the epstein-barr virus producer p3hr-1 and the epstein-barr virus genome-negative bjab lymphoblastoid cell lines, respectively. as the immune adhe ... | 1982 | 6313742 |
| [agarose selection of human lymphoid cells transformed in vitro by epstein-barr virus]. | 1982 | 6320706 | |
| two distant regions of the epstein-barr virus genome with sequence homologies have the same orientation and involve small tandem repeats. | the two regions of the epstein-barr virus genome (dsl and dsr) carrying homologous sequences at distant parts of the long unique region are described. cleavage of cloned dna containing the dsr region with restriction endonucleases revealed a so far unrecognized small tandem repeat of approximately 120 base pairs present in approximately 20 copies. heteroduplexes of the dna of two clones containing dsl and dsr respectively, visualized in the electron microscope by cytochrome c spreading, revealed ... | 1982 | 6325150 |
| human helper t cell factor(s) (thf). ii. induction of igg production in b lymphoblastoid cell lines and identification of t cell-replacing factor- (trf) like factor(s). | igg-pfc was induced in epstein-barr virus-transformed b lymphoblastoid cell lines (lcl) by the addition of allogeneic t cells. t cells involved in the induction of igg-pfc were shown to belong to the leu 3a+/2a- t cell subset. furthermore, partially purified soluble factors obtained from the culture supernatant of ppd-stimulated pleural t cells or pwm-stimulated tonsillar mononuclear cells was shown to induce igg-pfc in lcl across the major histocompatibility complex barrier. the induction of ig ... | 1982 | 6801125 |
| establishment of long-term monocyte suspension cultures from normal human peripheral blood. | the long-term suspension growth of normal, immature myeloid cells from fresh human cord blood was recently reported and required cells separated on supplemented discontinuous percoll gradients, growth in media containing hydrocortisone and vitamins d3, and gentle, continuous agitation (13). when normal adult bone marrow (six donors) or blood from epstein-barr virus (ebv)-seropositive donors (nine donors) was used as a source of fresh human leukocytes, only short-term proliferation of myeloid cel ... | 1982 | 6804592 |
| correlation between immunoglobulin light chain expression and variant translocation in burkitt's lymphoma. | burkitt's-type lymphomas-leukaemias (bl) are monoclonal proliferations of malignant b lymphocytes. irrespective of whether they carry the epstein-barr virus (ebv) genome, these tumour cells have been shown consistently to have one of the specific reciprocal chromosome translocations, t(8; 14), t(2; 8) or t(8; 22), involving the long arm of chromosome 8 (on 8q24) and chromosome 14, 2 or 22 (on 14q32, 2p12 and 22q11, respectively). the latter chromosomes have been shown recently to carry genes for ... | 1982 | 6806672 |
| acute non-a, non-b hepatitis in norway. clinical, epidemiological, and immunological characteristics in comparsion with hepatitis a. | serological analysis by radioimunoassay of sera from 297 patients hospitalized with acute non-toxic hepatitis was used for classification according to virus etiology. radioimmunoassays included tests for hepatitis b surface antigen (hbsag), antibody to hbsag (anti-hbs), antibody to hepatitis a virus (anti-hav), anti-hav of igm class, and antibody against cytomegalovirus (gmv) and epstein-barr virus. one patient with a significant rise in anti-cmv antibodies was classified as having cmv hepatitis ... | 1982 | 6817410 |
| post-transfusion non-a, non-b hepatitis in finland: a prospective study. | to study the frequency of post-transfusion non-a, non-b (nanb) hepatitis in finland, we have followed prospectively 65 patients after open-heart surgery. the total number of transfused blood units was 652. blood samples were obtained from the patients during the first 6 months after the operation and were studied for transaminase levels. the nanb diagnosis was based on exclusion of viral hepatitis of known etiology by serological tests for hepatitis a, b, cytomegalovirus and epstein-barr virus i ... | 1982 | 6818679 |
| a 53-kilodalton protein common to chemically and virally transformed cells shows extensive sequence similarities between species. | a heat-stable dna-binding protein with subunits of about 53 kilodaltons (kdal) was purified from two virally transformed human cell lines (epstein-barr virus-positive raji and namalwa) and two mouse tumor cell lines (methylcholanthrene-induced meth a sarcoma and ta3 mammary carcinoma). all four 53kdal proteins showed closely related total amino acid compositions, similar peptide maps, and identical nh2-terminal amino acid sequences for 20 residues. these 53-kdal proteins are therefore evolutiona ... | 1982 | 6952184 |
| a novel monocytoid cultured cell line, p31/fujioka, derived from acute monoblastic leukemia. | a novel cultured cell line, p31/fujioka, of monocytoid nature was established from leukemic cells in the peripheral blood of a seven-year-old boy with acute monoblastic leukemia. the p31/fujioka cells have abundant cytoplasm, an indented nucleus of monocytoid appearance, pseudopods detectable by electron microscopy and alpha-naphthyl butyrate esterase activity which is completely inhibited by naf, but they have no peroxidase activity. immunologically, the p31/fujioka cells possess fc gamma-recep ... | 1982 | 6962783 |
| malignant lymphoma of small cleaved lymphocytes of the follicular mantle zone. | we describe a highly unusual lymphocytic lymphoma. it appeared to originate in the mantle zones of hyperplastic follicles that had large reactive centers. the tumor cells in the mantle zone were small lymphocytes with cleaved or very irregular nuclei. they had coarse and abundant igm, kappa surface immunoglobulin markers, and receptors for complement. the tumor involvement was generalized at the time of discovery. the diagnosis of a malignant lymphoma was initially made with difficulty because o ... | 1982 | 7034814 |
| autologous bone marrow rescue in the treatment of advanced tumors of childhood. | high-dose multiagent chemotherapy followed by autologous marrow rescue was used in the treatment of 13 patients with stage iii or iv childhood tumors. encouraging results are being obtained in abdominal lymphoma (1/3 complete remissions (cr); rhabdomyosarcoma (2/4 cr); and retinoblastoma (1/2 cr). in neuroblastoma, the results are disappointing, with only one of four patients in cr; this patient developed a lymphoma associated with epstein-barr virus infection. marrow reconstitution was obtained ... | 1982 | 7037150 |
| decreased insulin binding in cultured lymphocytes from two patients with extreme insulin resistance. | [125i]insulin binding has been studied in two patients with extreme insulin resistance using cultured b-lymphocytes transformed with epstein-barr virus. a cell line from a female infant with leprechaunism had insulin binding which was decreased 90% below the lower limit of normal. lymphocytes from a young woman with type a extreme insulin resistance (associated with acanthosis nigricans and virilization) had insulin binding which was 80% depressed. in both cases, the defect in binding resulted f ... | 1982 | 7037823 |
| [undifferentiated carcinoma with lymphoid stroma (undifferentiated carcinoma nasopharyngeal type?). optical, electron microscopical and immunofluorescence study (author's transl)]. | a case of undifferentiated carcinoma with lymphoid stroma of the paired gland is reported in a chinese woman with positive epstein-barr virus serology. the histologic appearance of the tumor is very similar to undifferentiated carcinomas nasopharyngeal type (ucnt). ultrastructural study reveals features of epidermoid differentiation. immunofluorescence study shows numerous and predominant iga plasma cells in the stroma. the relationship between this tumor and the benign lymphoepithelial lesions ... | 1982 | 7042007 |
| lymphoblastoid lines and skin fibroblasts from patients with tuberous sclerosis are abnormally sensitive to ionizing radiation and to a radiomimetic chemical. | lymphoblastoid lines, derived by transforming peripheral blood lymphocytes with epstein-barr virus, and skin fibroblast lines were established from two patients with tuberous sclerosis. the number of viable lymphoblastoid cells was determined by their ability to exclude the vital dye trypan blue after their irradiation with x-rays or 254 nm ultraviolet light. the growth of fibroblasts was determined by their ability to form colonies after treatment with the radiomimetic, dna-damaging chemical n- ... | 1982 | 7057056 |
| characteristics of a brain lymphoma cell line derived from primary intracranial lymphoma. | a brain lymphoma cell line, designated tk, was established from biopsy material of a patient with primary intracranial lymphoma. the tk cell line grew in suspension with clumps of cells and consisted of primitive lymphoid cells. tk cells and original lymphoma cells from which the cell line was derived bore surface immunoglobulin and lacked epstein-barr virus-determined nuclear antigen. transplantation of the tk cells into immunosuppressed newborn hamsters gave rise to invasive growth of tumors w ... | 1982 | 7059907 |
| the american burkitt's lymphoma registry: eight years' experience. | four-hundred-twenty-one americans diagnosed as having burkitt's lymphoma (bl), 409 from the united states, were studied by the american bl registry to obtain information about the cause and control of this disease. of these 421 cases, 256 were confirmed by our pathologists as being morphologically indistinguishable from african bl, a relationship between age and organ involvement was observed; cervical lymph nodes, ileum, and nasopharynx were initial sites of involvement primarily in younger pat ... | 1982 | 7059918 |
| cytogenetic studies on four cases of non-endemic burkitt lymphoma. | cytogenetic studies carried out on four children with non-endemic burkitt lymphoma showed: 1) two with the typical translocation t(8;14)(q24;q23); 2) one with a variant t(2;8)(p11;q24); and 3) one with apparently normal chromosomes 8 and 14. additional chromosomal variation was present in all four patients. two were shown to have a duplication of part of the long arm of chromosome 1 (1q23 leads to 1q32 and 1q23 leads to 1q42). epstein barr virus studies on two patients showed that one was positi ... | 1982 | 7062897 |
| purification and biologic characterization of a major epstein barr virus-induced membrane glycoprotein. | the epstein barr virus membrane antigen (ma) complex is composed of three major high m.w. glycoproteins designated gp 300/350, gp 200/250, and gp 85/90. in the experiments reported in this paper, the gp 300/350 glycoprotein was purified from ma-positive extracts prepared from the b-95-8 cell line. this was accomplished by tandem combination of ion-exchange and lectin chromatography. it was routinely possible to purify this glycoprotein 400- 600-fold by this procedure. sera from animals immunized ... | 1982 | 7086144 |
| extra chromosome 12 and prognosis in chronic lymphocytic leukaemia. | peripheral blood lymphocytes from 22 consecutive patients with chronic lymphocytic leukaemia were stimulated with the polyclonal b-cell mitogens lipopolysaccharide from e. coli (lps) and epstein-barr virus (ebv). stimulation was successful for chromosome analysis in 14 patients. eleven patients had chromosomal aberrations and 7 of these had an extra chromosome 12. in 2 patients an extra chromosome 12 was the only abnormality, while additional aberrations were found in 5 patients. 3 patients had ... | 1982 | 7089476 |
| establishment and characterization of a human myeloma cell line (kmm-1). | a new cell line (kmm-1) was established from a subcutaneous plasmacytoma of a 62-year-old male with multiple myeloma. immunological studies indicated that cultured cells were derived from the same clone of myeloma cells in vivo: smeared cells were stained with fluorescein-conjugated globulin of antisera monospecific to lambda-chain, and lambda-chains in the cell extracts and in culture media were identical to the bence-jones protein found in the patient's urine. the cell line grew in suspension ... | 1982 | 7095894 |
| infectious mononucleosis in the athlete. diagnosis, complications, and management. | although almost always a benign, self-limiting disease, infectious mononucleosis accounts for considerable symptomatic illness in the young athlete and can, on occasion, be truly life-threatening. recognition of the syndrome "glandular fever," vis-a-vis infectious mononucleosis--fever, pharyngitis, lymphadenopathy, and splenomegaly, with characteristic changes in the peripheral blood leukocytes--dates back over a half a century. however, seroepidemiologic studies have only recently established i ... | 1982 | 7114352 |
| [lymphoepithelial carcinoma of the oropharynx: incidence and differential di]. | from 1960--1980, 452 malignant tumors of the oro- and nasopharynx were observed in the cologne university ent-clinic. 313 tumors of the oropharynx and 119 of the nasopharynx could be reclassified using the current classifications of upper respiratory tract tumors (who and cologne modification) and of malignant lymphomas (german kiel and rappaport classifications). the analysis showed a different distribution of histological diagnoses in the naso- and oropharynx. whereas the squamous cell carcino ... | 1982 | 7118592 |
| age and sex as factors influencing remission duration in african burkitt's lymphoma. | the frequency of burkitt's lymphoma peaks in children six to nine years of age and is twice as high in males as in females. to determine if age or sex had any relationship to outcome of therapy, the authors examined the experience of 387 patients treated for burkitt's lymphoma at the burkitt tumor project, accra, ghana, between 1966 and 1978. no age or sex relationship could be demonstrated with overall survival. however, among children without central nervous system disease who achieved an init ... | 1982 | 7137153 |
| familial chronic mononucleosis. | a syndrome of chronic mononucleosis occurred in two members of a family. symptoms were chronic malaise and fatigue; recurrent upper respiratory tract infections; and mild, variable immune abnormalities. intermittently positive heterophil titers were present for more than 2 years after acute infectious mononucleosis. epstein-barr-virus-specific antibodies were persistently abnormal. in the proband, the r component of the early antigen complex was present for 3 years and she never developed normal ... | 1982 | 7149489 |
| classification of cell lines derived from undifferentiated lymphomas according to their expression of complement and epstein-barr virus receptors: implications for the relationship between african and american burkitt's lymphoma. | 1982 | 7151335 | |
| burkitt's lymphoma in the tonsil. | a case of burkitt's lymphoma is reported in which both its location in waldeyer's ring and the age of the patient, 2 years, are rare. the disease is found in an endemic and a sporadic form. the endemic form especially is thought to be related to epstein barr virus infection. the treatment is chemotherapy combined with cytostatica, always containing cyclophosphamide it is one of the few human tumours which can be completely cured with cytostatica. | 1982 | 7152846 |
| secretion of immunoglobulins by human lymphocytes after infection with influenza virus. | the biosynthesis of igm by the epstein-barr virus-negative ramos lymphoblastoid cell line infected with an influenza a virus, fowl plague virus dobson strain (fpv-b), was investigated. the results show that fpv infection of ramos cells slightly inhibited overall cellular protein synthesis only at 24 h after infection, despite the synthesis of fpv-specific proteins. however, even at this time, the synthesis and secretion of igm were not affected by virus infection. secreted igm contained a reduce ... | 1982 | 7153765 |
| serum immunoglobulins in guillain-barré syndrome. | serum major class immunoglobulin concentrations were measured in 162 patients with guillain-barré syndrome (gbs) and 85 normal controls. significant elevations of igm, a and g were found in the gbs group. forty-four of 162 gbs patients (27%) had igm concentrations greater than two standard deviations above the control mean. gbs patients with high serum igm were young and 68% showed serologic evidence of recent infection with dna core agents, cytomegalovirus, epstein-barr virus, or mycoplasma pne ... | 1982 | 7161628 |
| epidemiology and prospects for a cancer registry in malaysia. | the history of efforts to establish a cancer registry in malaysia since 1961 is reviewed. in 1980, the staff of the institute for medical research in kuala lumpur was authorized to develop an official registry that would combine the resources of the various university faculties, the hospitals, research institutes, and the cancer society. special registries operate for oral precancerous conditions and for nasopharyngeal carcinoma (npc). the topics of recent epidemiological studies include: a revi ... | 1982 | 7167195 |
| long-term human cytolytic t-cell lines allospecific for hla-dr6 antigen are okt4+. | human allospecific cytotoxic t lymphocyte lines were established by weekly stimulation of peripheral blood lymphocytes with allogeneic epstein-barr virus-transformed lymphoblastoid cell lines in the presence of interleukin 2. the cytotoxic t lymphocyte lines were stimulated by either jy (which expresses hla-a,b and -dr) or daudi (which expresses hla-dr but not -a,b antigens). specificity of the effector cell lines was determined by antibody blocking and by patterns of cytolysis against a panel o ... | 1982 | 6980419 |
| cell lines derived from a human myelomonocytic leukaemia. | two cell lines have been isolated from separate samples, taken at the same time, from the peripheral blood of a case of myelomonocytic leukaemia. one of these (cess-b) is a typical b cell line obtained after treatment of the peripheral blood white cells with epstein-barr virus (ebv). the second line (rc-2) is a monocyte-macrophage type, originally isolated by 5 months continuous culture in the presence of colony stimulating factor (csf). subsequently, after further culture, this line has become ... | 1982 | 6980663 |
| establishment and cytological characteristics of two in vitro t-cell lines derived from a child with acute lymphatic leukemia and a man with adult t-cell leukemia in japan. | two permanent t-cell leukemia lines designated kh-1 and kh-2 were established from the peripheral blood of a 9-year-old boy with acute lymphoblastic leukemia and a 47-year-old man with adult t-cell leukemia (atl). no t-cell growth factor was used. kh-1 cells grew as single cells and kh-2 cells formed clusters in suspension culture. erosette formation, the absence of immunoglobulin determinants and epstein-barr-virus-associated nuclear antigen, and the presence of t-cell antigens revealed by mono ... | 1982 | 6982237 |
| two new acute lymphoblastic leukemia cell lines with early b-cell phenotypes. | two leukemic cell lines (697 and 207) were established from bone marrow cells obtained from children with all in relapse. these cell lines were positive for the common-all antigen (calla), the hla-dr (i.e., ia-like) antigen, and for cytoplasmic and surface igm heavy chains. the lines were negative for other immunoglobulin heavy chains and light chains. the lines had elevated levels of terminal deoxynucleotidyl transferase enzyme and expressed surface antigens found on normal myeloid-macrophage c ... | 1982 | 6982733 |
| characterization of a new chromosomal marker for acute lymphoblastic leukemia from a long-term cell line. | a bone marrow aspirate from a child with acute lymphoblastic leukemia (all) at first relapse was used to establish cell line # 697. the cultured line and marrow aspirates taken at initial diagnosis and first relapse were examined and compared. similarities in all patterns evaluated confirmed the leukemic origin of the line. morphologically, the cells were typically lymphoblastic. cytochemically, they were slightly acid phosphatase-positive and negative for peroxidase, asd chloroacetate esterase, ... | 1982 | 6983904 |
| receptors for the complement c3d component and the epstein-barr virus are quantitatively coexpressed on a series of b-cell lines and their derived somatic cell hybrids. | 1982 | 6983911 | |
| [non-a, non-b hepatitis. a progress report]. | the present state of the art in non-a, non-b hepatitis is reviewed. eight years of world-wide efforts in research have not yielded a definite characterization of agent(s) and markers of this viral infection. diagnosis is therefore established mainly by exclusion of the hepatitis viruses a and b, epstein-barr virus and cytomegalovirus, and drug-induced liver disease. currently available serological, electron microscopic, immune electron microscopic and clinical information on non-a, non-b hepatit ... | 1983 | 6443590 |
| human natural killer cells, activated lymphocyte killer cells, and monocytes possess similar cytotoxic mechanisms. | the relationship between the killing mechanisms of human natural killer (nk) cells, mitogen- and mixed-lymphocyte-culture-induced activated lymphocyte killer (alk) cells, and monocytes was investigated with a monoclonal antibody. the igg2 antibody 9.1c3 was prepared from mice immunized with purified human large granular lymphocytes and selected from clones that inhibited nk cell killing. the 9.1c3 antibody bound to all mononuclear cells but not to granulocytes or k562 cells, and it selectively b ... | 1983 | 6608725 |
| chromosome studies in stimulated lymphocytes of b-cell chronic lymphocytic leukemias. | using a sister chromatid differentiation technique, cell cycle study of stimulated lymphocytes of b-cell chronic lymphocytic leukemia (b-cll) revealed their cell cycle progression to be similar to that of normal lymphocytes stimulated by t-cell and various polyclonal b-cell activators (pba). the chromosome constitutions of stimulated lymphocytes in 62 patients with b-cll were examined using pba such as epstein-barr virus (ebv) and lipopolysaccharide w from e. coli 055:b5 (lps). of the 20 patient ... | 1983 | 6610624 |
| disorders of b cells and helper t cells in the pathogenesis of the immunoglobulin deficiency of patients with ataxia telangiectasia. | the pathogenesis of the immunoglobulin deficiency of 20 patients with ataxia telangiectasia was studied using an in vitro immunoglobulin biosynthesis system. 10 patients had no detectable iga in their serum as assessed by radial diffusion in agar and 3 had a reduced serum iga concentration. the peripheral blood mononuclear cells of 17 of the patients and 17 normal controls were cultured with pokeweed mitogen for 12 d and the immunoglobulin in the supernatants measured. the immunoglobulin synthes ... | 1983 | 6822665 |
| induction of epstein-barr virus early antigens by corticosteroids: inhibition by tpa and retinoic acid. | corticosteroids can induce the synthesis of ebv antigens in the burkitt lymphoma line daudi. as early as 12 h after application of the drug, an increase of ea-positive cells can be seen, the maximum induction being reached after 2 days. nanogram amounts per ml of hormone are sufficient for measurable effects. early antigen induction by corticosteroids does not require replication of viral dna. induction by corticosteroid differs from induction by other systems in two major respects: (1) it does ... | 1983 | 6826253 |
| multiple hepatitis attacks with immunothrombocytopenia in a heroin addict. | a 25-year-old heroin-addict had four episodes of acute hepatitis, each of them associated with thrombocytopenia. serologically, hepatitis a and b as well as cytomegalo- or epstein-barr virus infections were excluded. high levels of circulating immune complexes and antibodies reacting with aminophospholipids, a component of both liver cell and platelet membranes, were detected during the last two attacks of hepatitis, but were absent during remission. platelet-associated antibodies of the igm cla ... | 1983 | 6832695 |
| detection of hla antigens on lymphoblastoid and epithelial cell lines and cross-reactivity of hla-cw5 and hla-cw8. | antibody-dependent cell-mediated cytotoxicity was used to detect hla antigens on tissue cultured lymphoblastoid cells (phytohemagglutin blasts and epstein-barr virus lines) and transitional cell carcinomas. the results agreed with those obtained on fresh peripheral blood lymphocytes by conventional hla typing. the same hla antigens were detected on cells from an individual irrespective of their tissue origin or length of time in vitro. antibody-dependent cell-mediated cytotoxicity (adcc) showed ... | 1983 | 6841162 |
| inhibition of 12-o-tetradecanoylphorbol-13-acetate-induced induction in epstein-barr virus early antigen in raji cells. | retinol, 5 flavonoids, 3 steroids and 7 sweetening agents were studied for their effects on 12-o-tetradecanoylphorbol-13-acetate (tpa)-induced early antigen (ea) of epstein-barr virus (ebv) in raji cells. concomitant treatment of raji cells with tpa and retinol showed inhibition of ea induction. among flavonoids, quercetin resulted in effective inhibition of ea induction by tpa and alpha-naphthoflavone showed the weakly inhibitory effect. none of the other flavonoids such as rutin, catechin and ... | 1983 | 6850568 |
| o6-methylguanine removal by competent and incompetent human lymphoblastoid lines from the same male individual. | two cell lines, one proficient (mex+) and one deficient (mex-) in the ability to remove o6-methylguanine, have been isolated by epstein-barr virus-mediated transformation of a single blood sample obtained from a normal human male. extracts of untreated cells differ in their o6-methylguanine transferase (methyl acceptor protein) activity. although both lines arise from the same individual, they show great difference in their sensitivities to the toxic action of n-methyl-n'-nitro-n-nitrosoguanidin ... | 1983 | 6850639 |
| gianotti-crosti syndrome and epstein-barr virus infection. | 1983 | 6854440 | |
| epstein-barr virus infection and antibody synthesis in patients with multiple sclerosis. | we studied infectious and immune mechanisms in demyelinating disease. the clinical diagnosis in this study of 313 consecutive cases of multiple sclerosis (ms) was based on the clinical conclusions of two or more neurologists and definite abnormalities in csf igg. measurement of antibodies to six microbial agents was compared in 313 patients with ms and 406 controls in the same age range. using a standardized immunofluorescent antibody (ifa) technique, we found a significantly higher prevalence o ... | 1983 | 6860175 |
| erythema nodosum associated with infectious mononucleosis. | erythema nodosum is an immunoallergic epiphenomenon appearing during the course of various conditions; it has been exceptionally observed in association with infectious mononucleosis. a 9-year-old girl with clinical manifestations of infectious mononucleosis developed cutaneous nodules of erythema nodosum over the shins, which cleared after 12 days. diagnosis was based on the presence of atypical mononuclear cells, detection of antibodies against epstein-barr virus, and positive ox red blood cel ... | 1983 | 6862998 |
| primary infection by epstein-barr virus presenting as aplastic anemia. | 1983 | 6866059 | |
| viruses and human cancer. | the cause of human cancer is probably multifactorial and the role of viruses is unclear. the study of retroviruses has led to the identification of oncogenes responsible for transformation and tumor induction. human viruses associated with malignancies include the jc virus (associated with progressive multifocal leukoencephalopathy) and some adenoviruses. no human malignancies have been associated with the latter group. a number of herpes viruses of lower animals have been associated with malign ... | 1983 | 6870184 |
| antibody- and interferon-dependent killer cells are part of the nk cell receptor positive subpopulation of human peripheral blood cells. | cytotoxicity by human non-adherent peripheral blood lymphocytes was analysed after effector cell activation with either interferon (if) or by target cell specific igg antibodies (t-igg). four different cell lines were used as target cells that differed in susceptibility to natural killer cell (nk) activity; a highly susceptible t cell line (molt-4), a medium susceptible b lymphoma line (daudi), a resistant b cell line established by epstein-barr virus transformation (lcl-ls) and a resistant mous ... | 1983 | 6872324 |
| altered oligosaccharides as the initiating autoantigen in rheumatoid arthritis. | the linkage of rheumatoid arthritis (ra) to infection though vague has remained a persistent notion for the past fifty years. the hypotheses that streptococcus was responsible; then m. pneumoniae; the implication of a viral pathogenesis and, more especially, the epstein-barr virus, have all not withstood the test of koch's postulates. today, autoimmunity holds the field in the pathogenesis of ra. we know in fair detail what happens after failure of self-recognition develops. why does synovial ti ... | 1983 | 6877115 |
| viral antibodies in maternal and cord sera. | viral antibodies were determined in paired maternal and cord blood sera of 258 consecutive deliveries. antibodies to epstein-barr virus (ebv), cytomegalovirus (cmv), herpes simplex type 1 (hsv-1), adenovirus (adv), and varicella zoster (vzv) were tested by indirect immunofluorescence and to rubella (rub) by hemagglutination inhibition. analysis of the overall pattern of differences between maternal and cord blood showed a highly significant difference for all six viruses. analysis regarding indi ... | 1983 | 6877173 |
| gangliotriaosylceramide (asialo gm2), a glycosphingolipid marker for cell lines derived from patients with hodgkin's disease. | three cell lines, isolated from different patients with hodgkin's disease, were analyzed for their neutral glycosphingolipids by high performance liquid chromatography. on two of these lines gangliotriaosylceramide (asialo gm2) was identified by the comparison of retention time to authentic standards and by degradation with specific glycosidases. this glycosphingolipid was also serologically detected with a specific monoclonal antibody of the igm class. the third line, which according to other c ... | 1983 | 6886427 |
| characterization of lymphoid cells by two-dimensional mini gel electrophoresis of proteins. | cytosol proteins from normal lymphocytes, leukemic lymphocytes, and different cultured lymphoid cell lines were separated by two-dimensional mini gel electrophoresis. by staining with coomassie blue, specific protein patterns were obtained. very similar gel maps were produced by the cytosol proteins of chronic lymphocytic leukemia cells, hairy cells, and of in vitro grown b cells. protein 36/6.2 (molecular weight/isoelectric point), consistently present in these cells, could not be demonstrated ... | 1983 | 6572191 |
| [fatal infectious mononucleosis in a leukemic child in remission]. | the authors report the case of a 5 year-old girl who died from primary epstein-barr virus infection while in remission from acute lymphoblastic leukemia. there was a diffuse, polymorphic and polyclonal proliferation of b lymphocytes, associated with a strong histiocytic reaction with hemophagocytosis. the role of epstein-barr virus was ascertained by the appearance of specific antibodies and the finding of ebna antigen on liver smear. | 1983 | 6578707 |
| characterization of the phorbol 12,13-dibutyrate (p(bu)2) induced binding between human lymphocytes. | the mechanisms, cell surface structures, and cell types involved in the phorbol 12,13-dibutyrate (p(bu)2)-induced binding between human lymphocytes were studied. induction of cell aggregation by 20 min treatment with p(bu)2 required ca2+, an intact membrane, functional microfilaments, and the possible participation of an esterase or, less likely, a protease. trypsin-sensitive cell surface structures were needed and neuraminidase (nanase) treatment slightly increased the intercellular binding. re ... | 1983 | 6580088 |
| human monoclonal antibody to a neuroectodermal tumor antigen (ofa-i-2). | human igm kappa monoclonal antibody to human tumors of neuroectodermal origin was produced in the spent medium of an epstein-barr virus-transformed b-lymphoblastoid cell line, l72. chemically, the antigen was identified as ganglioside gd2 [gal nac beta 1----4 (neu ac alpha 2----8 neu ac alpha 2---3) gal beta 1----4 glc----ceramide]. twenty-seven mg of pure human igm were obtained from 10 liters of l72 spent medium using salt and hypotonic precipitation, ultracentrifugation, and sephacryl-s 300 s ... | 1983 | 6584083 |
| pre-b-cells in normal human bone marrow and in bone marrow from patients with leukemia in remission: persistent quantitative differences and possible expression of cell surface igm in vitro. | pre-b-cells are bone marrow lymphoid cells that lack surface immunoglobulin (sig-) but contain intracytoplasmic (c) igm heavy chains and are probably the immediate precursors of immature sigm+ b lymphocytes. to better understand early stages of b-cell development, immunofluorescence techniques were employed to identify pre-b-cells and b lymphocytes and to examine the expression of sigm in vitro by human marrow that had been previously depleted of b cells by immunoadsorption. marrow was derived f ... | 1983 | 6600632 |
| chromosome replication in normal and transformed human lymphocytes. | we analyzed the late-replication patterns of human b-lymphocyte chromosomes before and after transformation by epstein-barr virus. there were no statistically significant differences between normal cells and transformed cells derived from the same male individual; therefore, the order of termination of chromosome replication was unchanged by transformation. we also examined the replication patterns of t lymphocytes from the same donor and found no differences between normal b and t cells. | 1983 | 6601510 |
| increased frequency of rheumatoid factor precursor b lymphocytes after immunization of normal adults with tetanus toxoid. | vaccination of normal adults with tetanus toxoid induced a two-three-fold rise in the frequency of igm anti-igg (rheumatoid factor, rf) b lymphocytes inducible by the polyclonal b cell activator, epstein-barr virus. the increase in igm-rf precursors occurred earlier, was greater in magnitude, and was more sustained than the change in plasma igm-rf. it was associated with a rise in total igm levels, and correlated positively with the magnitude of the igg anti-tetanus antibody response, but not wi ... | 1983 | 6601551 |
| deficiency in epstein-barr virus receptors on b-lymphocytes of preleukemia patients. | lymphocytes from eight preleukemia patients were exposed to epstein-barr virus (ebv) in vitro in an attempt to establish lymphoblastoid cell lines. no signs of viral infection were detected, and no cell lines were obtained. studies using fluorescein-labeled ebv and flow cytometry revealed an unusual and consistent deficiency in ebv receptors in all patients examined. in control studies, about 15% of the unseparated lymphocytes from healthy donors bound fluorescein-labeled ebv. in spite of the la ... | 1983 | 6602654 |
| alternative pathway of complement activation by human lymphoblastoid b and t cell lines. | other investigators have reported activation of the alternative complement (c) pathway in homologous and heterologous serum by a variety of human lymphoblastoid b cell lines. their ability to activate the c was associated with epstein barr virus transformation of the cells. we report that some human lymphoblastoid t cell lines, lacking ebv-dna in their genome, do activate the human alternative c pathway with no participation of immunoglobulin or specific antibodies. nevertheless, activation meas ... | 1983 | 6604099 |
| chromosome abnormality in a human tcgf-dependent t-lymphocyte culture generated against autologous melanoma cells in vitro. | six t-cell growth promoting factor (tcgf)-dependent t-cell cultures and phytohemagglutinin-stimulated peripheral blood lymphocytes from a patient with metatastic malignant melanoma were karyotyped. three of the tcgf-dependent cultures were noncloned, started after in vitro sensitization against autologous malignant melanoma cells in tissue culture. a fourth was a subculture of one of these. the two remaining t-cell cultures were generated by in vitro sensitization of the same individual's lympho ... | 1983 | 6605190 |
| two non-t/non-b leukemia cell lines (thp-3-1 and thp-3-2) established from a patient at different stages of the disease. | two leukemia cell lines of different phenotypic expression were established from a single patient with non-t/non-b acute lymphocytic leukemia (all); one (thp-3-1) was derived from the peripheral blood taken on admission before chemotherapy and the other thp-3-2) during the terminal stage. both thp-3-1 and thp-3-2 cells were positive for terminal deoxynucleotidyl transferase (tdt) and ia-like antigen but negative for surface immunoglobulins (ig), nuclear antigen of epstein-barr virus (ebna) and r ... | 1983 | 6605598 |
| a novel hla-d/dr-like antigen specific for human b lymphoid cells. biochemical evidence for similarity to but nonidentity with known hla-d/dr antigens. | the polymorphic human b cell-specific antigen, 33.1, detected by a murine monoclonal antibody, was compared by genetics and structural analysis with known human ia antigens from a panel of dr homozygous epstein-barr virus-transformed b lymphoblastoid cell lines. cells homozygous for dr 1, 2, 4, 5, and w6 were positive, while cells that are dr3,3 or dr7,7 usually failed to express this antigen. mutant dr null, dc/mb-positive cells were 33.1 positive while dr null, dc/mb-negative cells failed to e ... | 1983 | 6606012 |
| [human monoclonal antibodies with human-mouse hybridomas]. | our trial to produce human monoclonal antibodies with human-mouse hybridomas is described. human spleen cells, in comparison with peripheral blood lymphocytes, bone marrow cells and lymphnode lymphocytes, seem to be the best partner to fuse with myeloma line ns-1 to obtain hybridomas. about sixty percent of hybridomas derived from human spleen cells at first produce human ig which in most cases belong to either igg or igm. fifty percent of hybridomas which had initially produced human ig continu ... | 1983 | 6606396 |
| acute cerebellar ataxia and hearing loss as initial symptoms of infectious mononucleosis. | a 16-year-old boy had symptoms and signs of cerebellar ataxia and hearing loss. he had no systemic manifestations of infectious mononucleosis and no lymphocytosis. results of a test for infectious mononucleosis were positive and there was a fourfold rise of serum antibody titer to epstein-barr virus capsid antigen. the patient recovered from ataxia but the hearing loss persisted. we reviewed the english literature and 15 cases of cerebellar ataxia associated with infectious mononucleosis were co ... | 1983 | 6625994 |
| estrogen receptor analysis in chronic lymphocytic leukemia. | estrogen receptor (er) determinations were performed on cytosol preparations of ficoll-hypaque density separated mononuclear cells from 11 patients with chronic lymphocytic leukemia (cll). the presence of er was noted in 8 of 11 specimens (73%). er ranged from 431 fmole/mg to 4.3 fmole/mg cytosol protein. two types of receptor subunits were observed at the 8s and 4s region of the sucrose gradient. in addition, 1 of 3 epstein-barr virus (ebv) transformed b-lymphoblastoid cell lines from healthy d ... | 1983 | 6626749 |
| a novel japanese burkitt's lymphoma cell line, p32/ishida, with a new variant chromosomal translocation (2;14). | a novel cultured cell line, p32/ishida, was established from a japanese 8-year-old boy with abdominal burkitt's lymphoma. the p32/ishida cells were proved to have immature b-cell phenotypes on the basis of immunological surface marker analysis: surface immunoglobulins (gamma, mu, kappa), ia-like antigen, b1 antigen, and common acute lymphoblastic leukemia antigen were positive. a small amount of igm, but no igg, could be detected in both cell extract and culture supernate of p32/ishida cells. cy ... | 1983 | 6667834 |