Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| ileorectal bypass performed entirely through a transanal route in a porcine model. | background: natural orifice transluminal endoscopic surgery (notes), a new frontier of minimally invasive surgery, uses the body's natural orifices to create an access for surgical procedures. this study aimed to verify the technical feasibility of ileorectal bypass performed entirely through a transanal access. methods: the procedure was performed on 10 domestic pigs, after which they were killed. a transanal endoscopic microsurgery (tem) device and endoscopic and laparoscopic instruments were ... | 2011 | 21484531 |
| partial protection against classical swine fever virus elicited by dendrimeric vaccine-candidate peptides in domestic pigs. | we report the immunogenicity of three dendrimeric peptide vaccine candidates for classical swine fever virus (csfv). each dendrimeric construct contained four copies of a b-cell epitope from the e2 glycoprotein of csfv [construct 1: e2 (694-712); 2: e2 (712-727); 3: e2 (829-842)] joined to a t-cell epitope from the ns3 protein (residues 1446-1460). intramuscular immunization of domestic pigs with the different constructs significantly reduced the clinical score after lethal challenge with csfv. ... | 2011 | 21496472 |
| experimental pig model of old myocardial infarction with long survival leading to chronic left ventricular dysfunction and remodeling as evaluated by pet. | a pig model of reduced left ventricular (lv) function and remodeling or chronic heart failure with long survival after myocardial infarction (mi) has not been established. the aim of this study was to evaluate the pathophysiologic status of a pig model of old mi using a series of pet studies. | 2011 | 21498524 |
| [extracorporeal cardiac shock wave therapy improved myocardial micro-vascular circulation after acute myocardial infarction at early stage in pigs]. | to explore the effect of low-energy extracorporeal shock wave therapy to improve myocardial micro-vascular circulation after acute myocardial infarction at the early stage in pig model. | 2011 | 21500558 |
| characterization of a novel porcine enterovirus in domestic pig in hungary. | porcine enteroviruses (pevs) of genus enterovirus are small, non-enveloped viruses with single-stranded, positive sense genomic rna, belonging to the family picornaviridae. the discovery of two distinct serotypes (pev9 and 10) was first reported in 1979. despite the sporadic detection and partial genome sequences of these viruses our knowledge about the prevalence and molecular epidemiology of pev types in domestic pigs is very deficient. in this study, we identified a novel pev from fecal sampl ... | 2011 | 21504800 |
| seroprevalence of toxoplasma gondii infection in domestic pigs in durango state, mexico. | abstract little is known concerning the prevalence of toxoplasma gondii infection in pigs in mexico. antibodies to t. gondii were determined in 1,077 domestic pigs in durango, mexico using the modified agglutination test (mat). two groups (a, b) of pigs were sampled: group a pigs (n=555) were raised in 3 geographical regions in durango state and group b pigs (n=522) were from sonora state but slaughtered in durango city. overall, antibodies to t. gondii were found in 137 (12.7%) of 1,077 pigs, w ... | 2011 | 21506843 |
| hypothesis on the dual origin of the mammalian subplate. | the development of the mammalian neocortex relies heavily on subplate. the proportion of this cell population varies considerably in different mammalian species. subplate is almost undetectable in marsupials, forms a thin, but distinct layer in mouse and rat, a larger layer in carnivores and big-brained mammals as pig, and a highly developed embryonic structure in human and non-human primates. the evolutionary origin of subplate neurons is the subject of current debate. some hypothesize that sub ... | 2011 | 21519390 |
| detection and phylogenetic characterization of human hepatitis e virus strains, czech republic. | to determine the origin of hepatitis e virus in the czech republic, we analyzed patient clinical samples. five isolates of genotypes 3e, 3f, and 3g were obtained. their genetic relatedness with czech strains from domestic pigs and wild boars and patient recollections suggest an autochthonous source likely linked to consumption of contaminated pork. | 2011 | 21529412 |
| successful transfection of genes using aav-2/9 vector in swine coronary and peripheral arteries. | background: gene therapy has attracted attention for its potential to treat several cardiovascular diseases. the use of adeno-associated viral (aav) vectors to facilitate therapeutic gene transfer to suppress intimal hyperplasia is a promising concept. the objective of this study was to analyze the in vivo transduction of a novel recombinant aav-2/9 vector with sm22α promoter, containing β-galactosidase gene (lacz) or green fluorescent protein (gfp) as reporter genes, to the medial layer smooth ... | 2011 | 21529824 |
| long-term evaluation of a selective retrograde coronary venous perfusion model in pigs (sus scrofa domestica). | the lack of suitable target vessels remains a challenge for aortocoronary bypass grafting in end-stage coronary heart disease. this study aimed to investigate the arterialization of cardiac veins as an alternative myocardial revascularization strategy in an experimental long-term model in pigs. selective retrograde perfusion of a coronary vein (aorta to coronary vein bypass, retrobypass) before ligation of the ramus interventricularis paraconalis (equivalent to the left anterior descending arter ... | 2011 | 21535926 |
| pcr-based molecular characterization and insilico analysis of food-borne trematode parasites paragonimus westermani, fasciolopsis buski and fasciola gigantica from northeast india using its2 rdna. | food-borne fluke infections/trematodiases are emerging as a major public health problem worldwide with over 40 million people affected and over 10% of world population at risk of infection. the major concentration of these infections is in southeast asian and western pacific regions, where the epidemiological factors (including the prevalent socio-cultural food habits) are conducive for transmission of these infections. the preponderance of these infections is usually in food deficit poor commun ... | 2011 | 21544167 |
| evaluation of post-farm-gate passive surveillance in swine for the detection of foot and mouth disease in australia. | pigs are considered high risk for the introduction and spread of foot and mouth disease (fmd) in australia. one of the most likely pathways of introduction of fmd into australia would be through the illegal importation of fmd-contaminated meat, which is then fed to feral or domestic pigs. locations where animals from different origins are commingled, such as livestock markets and abattoirs, pose a risk for disease spread. early detection of exotic diseases at these locations is crucial in limiti ... | 2011 | 21546105 |
| protection of european domestic pigs from virulent african isolates of african swine fever virus by experimental immunisation. | african swine fever (asf) is an acute haemorrhagic disease of domestic pigs for which there is currently no vaccine. we showed that experimental immunisation of pigs with the non-virulent ourt88/3 genotype i isolate from portugal followed by the closely related virulent ourt88/1 genotype i isolate could confer protection against challenge with virulent isolates from africa including the genotype i benin 97/1 isolate and genotype x uganda 1965 isolate. this immunisation strategy protected most pi ... | 2011 | 21549789 |
| effects of sustained abdominal aorta compression on coronary perfusion pressures and restoration of spontaneous circulation during cardiopulmonary resuscitation in swine. | objectives: the present study was undertaken to explore whether sustained abdominal aorta compression-cardiopulmonary resuscitation (saac-cpr), as a means, can raise coronary perfusion pressure (cpp) as well as restoration of spontaneous circulation (rosc) during cpr. in the present study, we hypothesised that saac-cpr elevates cpp during cpr and improves rosc, without causing liver laceration. methods: animals were randomised into one of two groups (standard cpr and saac-cpr). ten domestic swin ... | 2011 | 21550162 |
| integration between a percutaneous implant and the porcine small bowel. | inflammatory bowel diseases, cancer or trauma may require removal of all or part of the intestines, leaving the patient with a need to wear external stoma appliances for collection of bowel contents. by connecting the small bowel to a percutaneous port, equipped with a sealing lid, a fully continent and leak proof stoma can be created without a need for permanently wearing stoma appliance. the prerequisites for a connection between a permanent, transabdominal implant and a visceral organ are lar ... | 2011 | 21563306 |
| genotyping of mycoplasma hyopneumoniae in wild boar lung samples. | mycoplasma hyopneumoniae is the etiologic agent of enzootic pneumonia (ep), an important cause of disease-associated losses in swine production and a role of wild boar in recurrent infections can be supposed. genotypes of m. hyopneumoniae from wild boar are unknown but could indicate its role as a potential reservoir. therefore, 34 lung samples being pcr-positive for m. hyopneumoniae from wild boar from the geneva region in switzerland were assayed by genotyping using the p146 and multi-locus se ... | 2011 | 21571452 |
| thermodilution-derived indices for assessment of left and right ventricular cardiac function in normal and impaired cardiac function. | objective:: the aim of this study was to assess whether thermodilution-derived parameters of right and left ventricular cardiac function (right ventricular ejection fraction, global ejection fraction, cardiac function index) are able to track changes of cardiac contractile function and whether they are influenced by substantial preload reduction. design:: prospective animal study. setting:: university-affiliated animal research laboratory. subjects:: domestic pigs. interventions:: sixteen domest ... | 2011 | 21572331 |
| emergence of classical swine fever virus in israel in 2009. | classical swine fever (csf) re-emerged in israel in february 2009 after an absence of 62years. the outbreak occurred on a domestic pig farm in northern israel and affected domestic pigs and wild boar. on the basis of phylogenetic analysis of a 190 base pair fragment of the e2 glycoprotein gene, the israeli csf virus strain belonged to genotype 2.1 and was genetically most similar to a chinese csf virus strain. | 2011 | 21592830 |
| laparoscopic fixation of biologic mesh at the hiatus with fibrin or polyethylene glycol sealant in a porcine model. | background: the objective of this study was to determine the acute and chronic fixation strengths achieved by fibrin or polyethylene glycol (peg) sealants to secure biologic mesh at the esophageal hiatus in a porcine model. methods: for this study, 32 female domestic pigs were divided into four groups of 8 each. the four groups respectively received acute fibrin sealant, acute peg sealant, chronic fibrin sealant, and chronic peg sealant. laparoscopically, a 5.5 × 8.5-cm piece of biodesign surgis ... | 2011 | 21594740 |
| quantitative assessment of cardiac output and left ventricular function by noninvasive phase-contrast and cine mri: validation study with invasive pressure-volume loop analysis in a swine model. | purpose: to validate noninvasive cardiac output measurements of phase-contrast magnetic resonance imaging (pc-mri) and cine mri using an invasive pressure-volume (pv) loop technique on a swine model. materials and methods: we compared three methods for evaluating cardiac function at rest and under pharmaceutical low-dose inotropic infusion conditions: 1) phase-contrast mri, 2) cine mri, and 3) pv loop relationship. these measurements were made in 14 domestic pigs under rest conditions. identical ... | 2011 | 21598342 |
| [artyfechinostomum sufratyfex lane, 1915 (trematoda:echinostomatidae), a parasite of mammals in southern vietnam]. | trematoda artyfechinostomumn sufratyfex lane, 1915 was found in different mammal species of southern vietnam. original measurements of this species from domestic pig, black rat, and asian palm civet are given. | 2011 | 21598669 |
| evolution of endogenous retroviruses in the suidae: evidence for different viral subpopulations in african and eurasian host species. | abstract: background: porcine endogenous retroviruses (pervs) represent remnants of an exogenous form that have become integrated in the domestic pig (sus scrofa) genome. although they are usually inactive, the capacity of gamma1 ervs to infect human cells in vitro has raised concerns about xenotransplantation because the viruses could cross the species barrier to humans. here we have analyzed the evolution of gamma1 ervs in ten species of suidae (suids, pigs and hogs) from eurasia and africa us ... | 2011 | 21609472 |
| parenteral aluminum induces liver injury in a newborn piglet model. | parenteral nutrition-associated cholestasis remains a significant problem, especially for the surgical neonate. aluminum is a toxic element known to contaminate parenteral nutrition. we hypothesize that parenterally administered aluminum causes liver injury similar to that seen in parenteral nutrition-associated cholestasis. | 2011 | 21616246 |
| the consumption of glucose during ex vivo lung perfusion correlates with lung edema. | ex vivo lung perfusion (evlp) has been recently proposed to recondition organs before transplantation from donors with marginal or unacceptable features. the aim of our investigation was to explore glucose consumption during evlp. | 2011 | 21620034 |
| long-term treatment with nifedipine suppresses coronary hyperconstricting responses and inflammatory changes induced by paclitaxel-eluting stent in pigs in vivo: possible involvement of rho-kinase pathway. | aims accumulating evidence indicates that coronary vasoconstricting responses are enhanced at the edges of coronary segment implanted with a drug-eluting stent (des) compared with a bare-metal stent (bms) in humans. we have recently demonstrated that rho-kinase pathway plays an important role in des-induced coronary hyperconstricting responses associated with inflammatory changes in pigs in vivo. this study examined whether long-term treatment with calcium channel blocker suppresses des-induced ... | 2011 | 21624903 |
| microdialysis assessment of percutaneous penetration of ketoprofen after transdermal administration to hairless rats and domestic pigs. | the study was performed to evaluate the percutaneous penetration of ketoprofen after transdermal administration using a microdialysis technique in pigs, in comparison with rats. ketoprofen release from patches was determined by analysis of the remaining drug content after application to hairless rats and pigs. skin and knee joint penetration of ketoprofen was tested by microdialysis, and recovery was determined by retrodialysis. residual rates in hairless rats and pigs were 68.1 -¦ 1.6% and 81.7 ... | 2011 | 21397690 |
| validation of an automated method for salivary alpha-amylase measurements in pigs (sus scrofa domesticus) and its application as a stress biomarker. | the aim of the current study was to validate an automated spectrophotometric method for salivary alpha-amylase measurement in pigs and evaluate its possible application as a noninvasive stress biomarker. the analytical validation included intra- and interassay precision, linearity under dilution, and limit of detection. in addition, to study the possible use of salivary alpha-amylase as a possible stress marker, 12 crossbred growing pigs of 3-4 months of age were subjected to restraint stress by ... | 2011 | 21398448 |
| intracoronary adiponectin at reperfusion reduces infarct size in a porcine myocardial infarction model. | reperfusion injury (ri) remains an important limitation of myocardial revascularization. the aim of the present study was to evaluate the influence of the intracoronary injection of adiponectin on ri and cardiomyocyte death in a porcine myocardial infarction model. acute infarction in 14 polish domestic pigs was induced by inflation of an over the wire balloon (otw) catheter in the medial left anterior descending artery for 60-ámin. the study group consisted of 7 pigs in which intracoronary adip ... | 2011 | 21399860 |
| second prize: in-vivo physiological impact of alpha blockade on the porcine ureter with distal ureteral obstruction. | alpha blockade has been well described clinically for expulsion of distal ureteral stone. however, the in-vivo effects of a-blockade on ureteral dynamics during stone passage are not clear. we studied the effects of oral alfuzosin on ureteral pressure and peristalsis in a distally obstructed porcine ureter. | 2011 | 21401393 |
| vardenafil effect on ureteric smooth muscle: in vitro study in porcine model. | phosphodiesrase type 5 inhibitors have been recently reported to induce a relaxing effect on ureteral smooth muscle. we conducted an in vitro study to elucidate the relaxing effect of various doses of vardenafil on the porcine ureter. moreover, we propose a porcine ureter model for the evaluation of the effect of different substances on the ureter. | 2011 | 21401398 |
| age-related changes in corticosteroid receptor expression and monoamine neurotransmitter concentrations in various brain regions of postnatal pigs. | negative early life experience may be associated with altered functioning of stress-related systems and may increase vulnerability to diseases later in life. corticosteroids are important mediators of homeostasis and stress and exert their effects via two receptors, the mineralocorticoid receptor (mr) and the glucocorticoid receptor (gr), and through the glucocorticoid-metabolizing enzymes 11ß-hydroxysteroid dehydrogenase (11ß-hsd) types 1 and 2 in a brain-region-specific manner. however, relati ... | 2011 | 21416484 |
| the effect of combined delivery of recombinant human bone morphogenetic protein-2 and recombinant human vascular endothelial growth factor 165 from biomimetic calcium-phosphate-coated implants on osseointegration. | objectives: the delivery of growth factors for enhanced osseointegration depends on the effectiveness of the carrier systems at the bone-implant interface. this study evaluated the effect of solo and dual delivery of recombinant human bone morphogenetic protein-2 (rhbmp-2) and recombinant human vascular endothelial growth factor (rhvegf(165) ) from biomimetically octacalcium phosphate-coated implants on osseointegration. materials and methods: biomimetic implants, bearing either a single growth ... | 2011 | 21418332 |
| foot-and-mouth disease in feral swine: susceptibility and transmission. | experimental studies of foot-and-mouth disease (fmd) in feral swine are limited, and data for clinical manifestations and disease transmissibility are lacking. in this report, feral and domestic swine were experimentally infected with fmdv (a24-cruzeiro), and susceptibility and virus transmission were studied. feral swine were proved to be highly susceptible to a-24 cruzeiro fmd virus by intradermal inoculation and by contact with infected domestic and feral swine. typical clinical signs in fera ... | 2011 | 21418546 |
| welfare assessment in porcine biomedical research - suggestion for an operational tool. | in recent years, increasing interest in using the pig (sus scrofa) for biomedical research has become evident. today, the pig is considered an advantageous alternative animal model for various human diseases and conditions. however, even though a considerable amount of biomedical research has been done on pigs, hardly any studies include systematic welfare assessment. still, it is essential to assess welfare of laboratory pigs, both domestic pig breeds and smaller purpose-bred breeds, as (1) sci ... | 2011 | 21420697 |
| effects of local intracoronary paclitaxel delivery using the remedy transport catheter on neointimal hyperplasia after stent implantation in a porcine model. | to assess the effects of local paclitaxel delivery using the remedy catheter on neointimal hyperplasia in a porcine model and compare these results to commercially available bms and biodegradable polymer-coated paclitaxel-eluting stents (bp-pes). | 2011 | 21421185 |
| prevalence of classical swine fever virus in india: a 6-year study (2004-2010). | a study was undertaken regarding the prevalence of classical swine fever virus (csfv) antibodies and antigens in sera and suspected tissue samples of domestic pigs. the samples were received between january 2004 and september 2010. a total of 594 serum samples from 12 states and 287 tissue samples from 13 states of india were tested using commercial enzyme-linked immunosorbent assay (elisa) kits. the mean prevalence of csfv antibodies in suspected sera was 63.3% (376/594), whereas 76.7% (220/287 ... | 2011 | 21429182 |
| behavioural and cardiac responses towards conspecific distress calls in domestic pigs (sus scrofa). | in domestic pigs, vocalisation can be an indicator of distress and negative emotional states. it might play a role in the transfer of emotion between individuals ('emotional contagion' or 'empathy'), which could result in impaired animal welfare on a group level based on the distress in an individual member of the group. the aim of this study was to characterise the responses of pigs to conspecific distress calls. we performed a playback experiment in an open arena with 24 juvenile german landra ... | 2011 | 21439307 |
| comparative analysis of osseointegration of titanium implants with acid-etched surfaces and different biomolecular coatings. | objectives: an increasing trend toward implantation in complex cases, as well as early loading, is beginning to emerge in dental implantology. long-term stability of the inserted implants greatly depends on the osseointegration process. although there are numerous current research efforts aimed at functionalizing implant surfaces, no single factor has proved to be beneficial for osseointegration. the aim of the present study was to investigate whether a combination coating of collagen i and diff ... | 2011 | 21441047 |
| prevalence of antibody to toxoplasma gondii and trichinella spp. in feral pigs (sus scrofa) of eastern north carolina. | feral pigs (sus scrofa) survive in many climates, reproduce year-round, and are dietary generalists. in the united states, the size and range of the feral pig population has expanded, resulting in greater interaction with humans and domestic swine and increased potential for disease transmission. we conducted a serosurvey in feral pigs from eastern north carolina to determine exposure to the zoonotic parasites, toxoplasma gondii and trichinella spp. between september 2007 and march 2009, blood s ... | 2011 | 21441186 |
| semi-preparative isolation and purification of three tauro-conjugated cholic acids from pulvis fellis suis by hsccc coupled with elsd detection. | coupled with evaporative light scattering detection, a high-speed counter-current chromatography (hsccc) method was applied to the separation and purification of three tauro-conjugated cholic acids of taurochenodeoxycholic acid (tcdca), taurohyodeoxycholic acid (thdca) and taurohyocholic acid (thca) from pulvis fellis suis (pig gallbladder bile) for the first time. the two-phase solvent system composed of chloroform-methanol-water-acetic acid (4:4:2:0.3, v/v/v/v) was selected for the one-step se ... | 2011 | 21442029 |
| the diameter of anodic tio(2) nanotubes affects bone formation and correlates with the bone morphogenetic protein-2 expression in vivo. | introduction: recently, it has been demonstrated that the nanoscale environment is a critical factor for cellular behaviour. it has been shown that the diameter of tio(2) nanotube layers controls the cellular behaviour of cells involved in the bone-forming process in vitro. therefore, the aim of the present study was to investigate the effects of the diameter of tio(2) nanotubes on peri-implant bone formation and the expression of bone matrix proteins in vivo. materials and methods: ninety exper ... | 2011 | 21443609 |
| [standardized selection and its consequences for genetic population structure (examplified by sus scrofa l.)]. | type of selection for total fitness, specific for domestic animals and termed by us standardizing selection, is discussed. consequences of such selection for the population structure genetic are considered taking a population of domestic pigs as an example. examples of effective and ineffective standardizing selection are presented. specific features of genetic determination often quantitative traits detected in analysis of standardizing selection are discussed. | 2011 | 21446188 |
| combined chemical and optical methods for monitoring the early decay stages of surrogate human models. | as the body decays shortly after death, a variety of gases and volatile organic compounds (vocs) constantly emanate. ethical and practical reasons limit the use of human corpses in controlled, time-dependent, intervening experiments for monitoring the chemistry of body decay. therefore the utilization of pig carcasses serves as a potential surrogate to human models. the aim of this work was to study buried body decay in conditions of entrapment in collapsed buildings. six domestic pigs were used ... | 2011 | 21450424 |
| species-specific variation in rela underlies differences in nf-?b activity: a potential role in african swine fever pathogenesis. | african swine fever virus (asfv) is a highly infectious disease of domestic pigs, with virulent isolates causing a rapidly fatal hemorrhagic fever. in contrast, the porcine species endogenous to africa tolerate infection. the ability of the virus to persist in one host while killing another genetically related host implies that disease severity may be, in part, modulated by host genetic variation. to complement transcription profiling approaches to identify the underlying genetic variation in th ... | 2011 | 21450812 |
| quantitative computed tomography in porcine lung injury with variable versus conventional ventilation: recruitment and surfactant replacement. | biologically variable ventilation improves lung function in acute respiratory distress models. if enhanced recruitment is responsible for these results, then biologically variable ventilation might promote distribution of exogenous surfactant to nonaerated areas. our objectives were to confirm model predictions of enhanced recruitment with biologically variable ventilation using computed tomography and to determine whether surfactant replacement with biologically variable ventilation provides ad ... | 2011 | 21460711 |
| vaccination with viral protein-mimicking peptides postpones mortality in domestic pigs infected by african swine fever virus. | periodic outbreaks of african swine fever virus (asfv) infection around the world threaten local populations of domestic pigs with lethal disease and provide grounds for pandemic spread. effective vaccination may bring this threat under control. we investigated the effectiveness of select peptides mimicking viral proteins in establishing a protective immune response. forty-six synthetic peptides based on the analysis of the complete nucleotide sequence of asfv were tested for immunogenicity in m ... | 2011 | 21468582 |
| suture-free anastomosis of the colon. experimental comparison of two cyanoacrylate adhesives. | we explored the potential of two cyanoacrylate tissue adhesives for constructing colonic anastomoses. | 2011 | 21229329 |
| identification of a second gene associated with variation in vertebral number in domestic pigs. | the number of vertebrae in pigs varies and is associated with body size. wild boars have 19 vertebrae, but european commercial breeds for pork production have 20 to 23 vertebrae. we previously identified two quantitative trait loci (qtls) for number of vertebrae on sus scrofa chromosomes (ssc) 1 and 7, and reported that an orphan nuclear receptor, nr6a1, was located at the qtl on ssc1. at the nr6a1 locus, wild boars and asian local breed pigs had the wild-type allele and european commercial-bree ... | 2011 | 21232157 |
| microrna expression profiling of the porcine developing brain. | micrornas are small, non-coding rna molecules that regulate gene expression at the post-transcriptional level and play an important role in the control of developmental and physiological processes. in particular, the developing brain contains an impressive diversity of micrornas. most microrna expression profiling studies have been performed in human or rodents and relatively limited knowledge exists in other mammalian species. the domestic pig is considered to be an excellent, alternate, large ... | 2011 | 21253018 |
| distinctive differences in long terminal repeat sequences between γ1 endogenous retroviruses of african and eurasian suid species. | diversity of long terminal repeats (ltrs) from γ1 endogenous retroviruses (ervs) was analysed by dna sequencing in 10 species of the family suidae (suids, pigs and hogs). phylogenetic analysis separated ltr sequences into two groups which correlated approximately with either the previously described cluster i and iii, or the clusters ii, iv and v. interestingly, a specific ltr exhibiting a novel molecular rearrangement was identified exclusively within african host species when compared to ltrs ... | 2011 | 21256982 |
| shen-fu injection attenuates postresuscitation myocardial dysfunction in a porcine model of cardiac arrest. | to investigate the effect of shen-fu injection (sfi) for the management of postresuscitation myocardial dysfunction in a porcine model of cardiac arrest. ventricular fibrillation was induced electrically in anesthetized domestic swine. after 4 min of untreated ventricular fibrillation, cardiopulmonary resuscitation was initiated. sixteen successfully resuscitated pigs were randomized to receive a continuous infusion of either sfi (0.24 mg/min) or saline placebo for 6 h, beginning 15 min after re ... | 2011 | 21263380 |
| differential mrna expression of genes in the porcine adrenal gland associated with psychosocial stress. | to gain insight into the adrenal stress response, we analysed differential mrna expression of genes associated with psychosocial stress in the pig (sus scrofa domestica). various levels of psychosocial stress were induced by mixing groups of unfamiliar pigs with different aggressiveness. we selected two experimental groups for comparison, each comprising eight animals, which differed significantly in aggressive behaviour and plasma cortisol levels. to identify differentially expressed genes, we ... | 2011 | 21266515 |
| oct4-enhanced green fluorescent protein transgenic pigs: a new large animal model for reprogramming studies. | the domesticated pig has emerged as an important tool for development of surgical techniques, advancement of xenotransplantation, creation of important disease models, and preclinical testing of novel cell therapies. however, germ line-competent pluripotent porcine stem cells have not yet been derived. this has been a major obstacle to genetic modification of pigs. the transcription factor oct4 is essential for the maintenance of pluripotency and for reprogramming somatic cells to a pluripotent ... | 2011 | 21126163 |
| experimental infection of common warthogs (phacochoerus africanus) and bushpigs (potamochoerus larvatus) with classical swine fever virus. i: susceptibility and transmission. | an incursion of classical swine fever virus (csfv) into the domestic pig population in south africa, identified in 2005, raised the concern that infection might spread to wildlife species and be maintained in these hosts. this study sought to determine whether two wildlife suidae species present in south africa, the bushpig (potamochoerus larvatus) and the common warthog (phacochoerus africanus), could support productive csfv infection. both species could be infected with csfv and transmitted in ... | 2011 | 21294855 |
| the role of movement restrictions and pre-emptive destruction in the emergency control strategy against csf outbreaks in domestic pigs. | classical swine fever (csf) outbreaks in domestic pig herds lead to the implementation of standard control measures according to legislative regulations. ideal outbreak control entails the swift and efficient culling of all pigs on premises detected positive for csf virus. often all pig holdings around the detected cases are pre-emptively destroyed to exclude transmission into the neighbourhood. in addition to these measures, zones are defined in which surveillance and protection measures are in ... | 2011 | 21300412 |
| optical nerve detection by diffuse reflectance spectroscopy for feedback controlled oral and maxillofacial laser surgery. | laser surgery lacks haptic feedback, which is accompanied by the risk of iatrogenic nerve damage. it was the aim of this study to investigate diffuse reflectance spectroscopy for tissue differentiation as the base of a feedback control system to enhance nerve preservation in oral and maxillofacial laser surgery. | 2011 | 21310023 |
| neuronal genes for subcutaneous fat thickness in human and pig are identified by local genomic sequencing and combined snp association study. | obesity represents a major global public health problem that increases the risk for cardiovascular or metabolic disease. the pigs represent an exceptional biomedical model related to energy metabolism and obesity in humans. to pinpoint causal genetic factors for a common form of obesity, we conducted local genomic de novo sequencing, 18.2 mb, of a porcine qtl region affecting fatness traits, and carried out snp association studies for backfat thickness and intramuscular fat content in pigs. in o ... | 2011 | 21311593 |
| combining information from surveys of several species to estimate the probability of freedom from echinococcus multilocularis in sweden, finland and mainland norway. | the fox tapeworm echinococcus multilocularis has foxes and other canids as definitive host and rodents as intermediate hosts. however, most mammals can be accidental intermediate hosts and the larval stage may cause serious disease in humans. the parasite has never been detected in sweden, finland and mainland norway. all three countries require currently an anthelminthic treatment for dogs and cats prior to entry in order to prevent introduction of the parasite. documentation of freedom from e. ... | 2011 | 21314948 |
| assessment of interactions between african swine fever virus, bushpigs (potamochoerus larvatus), ornithodoros ticks and domestic pigs in north-western madagascar. | since its introduction in madagascar in 1998, african swine fever (asf) has severely affected national pig production and persists as a common disease in that country. two of its natural hosts in the african continent, the bushpig (potamochoerus larvatus) and tick vectors of the ornithodoros moubata complex, are reported in west and central regions of the island. however, their role in the maintenance and transmission of the virus has been insufficiently studied. in this work, we tried to assess ... | 2011 | 21320295 |
| a long-term serological survey on aujeszky's disease virus infections in wild boar in east germany. | summarybetween 1985 and 2008, a total of 102 387 wild boar sera originating from eastern germany covering an area of 108 589 km2 were tested for the presence of aujeszky's disease virus (adv)-specific antibodies. from 1985 until 1991 and from 1992 until 2008, wild boar sera were exclusively investigated using either conventional seroneutralization assays (n=39 621) or commercial gb and full antigen elisas (n=62 766), respectively. spatial-temporal analysis revealed an increasing adv seroprevalen ... | 2011 | 21320372 |
| discovery of hepatitis e: the epidemic non-a, non-b hepatitis 30 years down the memory lane. | hepatitis e was first recognised during an epidemic of hepatitis, which occurred in kashmir valley in 1978. the epidemic involved an estimated 52,000 cases of icteric hepatitis with 1700 deaths. the disease had unique clinical and epidemiological features. the epidemic was water-borne with highly compressed epidemic curve. following the epidemic, secondary waves of hepatitis did not occur. clinical profile was characterized by cholestasis in around 20% of patients. the disease predominantly occu ... | 2011 | 21320558 |
| markers of progenitor cell recruitment and differentiation rise early during ischemia and continue during resuscitation in a porcine acute ischemia model. | clinical administration of bone marrow-derived stem cells in the setting of acute myocardial infarction (ami) leads to improved left ventricular ejection fraction. thymosin beta-4 (tb4) and vascular endothelial growth factor (vegf) are linked to adult epicardial progenitor cell mobilization and neovascularization and is cardioprotective after myocardial ischemia. this study investigated the time course of tb4 and vegf during ami, cardiac arrest, and resuscitation. fifteen anesthetized and instru ... | 2011 | 21332365 |
| vascular access port implantation and serial blood sampling in a gottingen minipig (sus scrofa domestica) model of acute radiation injury. | threats of nuclear and other radiologic exposures have been increasing, but no countermeasure for acute radiation syndrome has been approved by regulatory authorities. because of their similarity to humans in regard to physiology and anatomy, we are characterizing gottingen minipigs as a model to aid the development of radiation countermeasures. irradiated minipigs exhibit immunosuppression, severe thrombocytopenia, vascular leakage, and acute inflammation. these complications render serial acqu ... | 2011 | 21333166 |
| microsurgical cerebral aneurysm training porcine model. | the authors developed a simple reproducible technique for aneurysm creation and adapted it to mimic intracranial dissection conditions using glue application as a pseudo-arachnoid type layer. ten 1-2-month-old healthy domestic swine were employed under general anesthesia. a novel technique for bifurcation aneurysm creation was developed using two arteries and a vein. after aneurysm creation, diluted sulfuric acid was applied on the dome with a micropipette to increase aneurysm fragility in selec ... | 2011 | 21339668 |
| epigenetic reprogramming in the porcine germ line. | epigenetic reprogramming is critical for genome regulation during germ line development. genome-wide demethylation in mouse primordial germ cells (pgc) is a unique reprogramming event essential for erasing epigenetic memory and preventing the transmission of epimutations to the next generation. in addition to dna demethylation, pgc are subject to a major reprogramming of histone marks, and many of these changes are concurrent with a cell cycle arrest in the g2 phase. there is limited information ... | 2011 | 21352525 |
| sodium nitroprusside enhanced cardiopulmonary resuscitation improves survival with good neurological function in a porcine model of prolonged cardiac arrest. | to assess the effectiveness of sodium nitroprusside (snp)-"enhanced" cardiopulmonary resuscitation (snpecpr) on 24-hr survival rates compared to standard cpr in animals after cardiac arrest. snpecpr consists of large intravenous snp bolus doses during cpr enhanced by active compression-decompression cpr, an inspiratory impedance threshold device (itd), and abdominal binding (ab). the combination of active compression-decompression cpr+itd+ab without snp will be called "enhanced" or ecpr. | 2011 | 21358401 |
| nonlinear microscopy, infrared, and raman microspectroscopy for brain tumor analysis. | contemporary brain tumor research focuses on two challenges: first, tumor typing and grading by analyzing excised tissue is of utmost importance for choosing a therapy. second, for prognostication the tumor has to be removed as completely as possible. nowadays, histopathology of excised tissue using haematoxylin-eosine staining is the gold standard for the definitive diagnosis of surgical pathology specimens. however, it is neither applicable in vivo, nor does it allow for precise tumor typing i ... | 2011 | 21361676 |
| the response of cranial biomechanical finite element models to variations in mesh density. | finite element (fe) models provide discrete solutions to continuous problems. therefore, to arrive at the correct solution, it is vital to ensure that fe models contain a sufficient number of elements to fully resolve all the detail encountered in a continuum structure. mesh convergence testing is the process of comparing successively finer meshes to identify the point of diminishing returns; where increasing resolution has marginal effects on results and further detail would become costly and u ... | 2011 | 21370496 |
| identification of low and high frequency ranges for heart rate variability and blood pressure variability analyses using pharmacological autonomic blockade with atropine and propranolol in swine. | understanding autonomic nervous system functioning, which mediates behavioral and physiological responses to stress, offers great potential for assessing farm animal stress and welfare. evaluation of heart rate variability (hrv) and blood pressure variability (bpv), using time and frequency domain analyses may provide a sensitive and reliable measure of affective states and stress-mediated changes in sympathetic and parasympathetic tones. the aim of this research was to define low (lf) and high ... | 2011 | 21281655 |
| molecular mechanism by which amp-activated protein kinase activation promotes glycogen accumulation in muscle. | during energy stress, amp-activated protein kinase (ampk) promotes glucose transport and glycolysis for atp production, while it is thought to inhibit anabolic glycogen synthesis by suppressing the activity of glycogen synthase (gs) to maintain the energy balance in muscle. paradoxically, chronic activation of ampk causes an increase in glycogen accumulation in skeletal and cardiac muscles, which in some cases is associated with cardiac dysfunction. the aim of this study was to elucidate the mol ... | 2011 | 21282366 |
| coronary vein infusion of multipotent stromal cells from bone marrow preserves cardiac function in swine ischemic cardiomyopathy via enhanced neovascularization. | few reports have examined the effects of adult bone marrow multipotent stromal cells (mscs) on large animals, and no useful method has been established for msc implantation. in this study, we investigate the effects of msc infusion from the coronary vein in a swine model of chronic myocardial infarction (mi). mi was induced in domestic swine by placing beads in the left coronary artery. bone marrow cells were aspirated and then cultured to isolate the mscs. at 4 weeks after mi, mscs labeled with ... | 2011 | 21283079 |
| discovery of porcine micrornas in multiple tissues by a solexa deep sequencing approach. | the domestic pig (sus scrofa) is an important economic animal for meat production and as a suitable model organism for comparative genomics and biomedical studies. in an effort to gain further identification of mirnas in the pig, we have applied the illumina solexa sequencing technology to carry out an in-depth analysis of the mirna transcriptome in a pool of equal amounts of rna from 16 different porcine tissues. from this data set, we identified 437 conserved and 86 candidate novel mirna/mirna ... | 2011 | 21283541 |
| the parameters of the porcine eyeball. | the eye of the domestic pig (sus scrofa domestica) is an ex vivo animal model often used in vision sciences research (retina studies, glaucoma, cataracts, etc.). however, only a few papers have compiled pig eye anatomical descriptions. the purpose of this paper is to describe pig and human eye anatomical parameters to help investigators in their choice of animal model depending on their study objective. | 2011 | 21287191 |
| different enteropathogenic yersinia strains found in wild boars and domestic pigs. | yersinia enterocolitica and yersinia pseudotuberculosis strains isolated from wild boars and fattening pigs were characterized and compared with each other. in wild boars, ail-positive y. enterocolitica strains belonged to bioserotypes 4/o:3 (36%, 5/14), 2/o:9 (29%, 4/14), and 2/o:5,27 (21%, 3/14). additionally, two ail-positive strains were untypable. among fattening pigs, the bioserotype 4/o:3 was dominating (91%, 71/78), and bioserotypes 2/o:5,27 (8%, 6/78) and 2/o:9 (1%, 1/78) were rare. inv ... | 2011 | 21288132 |
| trpml2 and the evolution of mucolipins. | trpml2, the polypeptide product of the gene trpml2 (aka mcoln2), is a member of the trpml or mucolipin branch of the trp super family of ion channels. although no known agonists have been discovered, the wild type channel gives basal currents when heterologously expressed in drosophila (s2) cells and is constitutively active in mammalian cells when bearing a cell degeneration-causing, proline to alanine substitution in the fifth trans-membrane domain. trpml2 forms channels that are inwardly rect ... | 2011 | 21290298 |
| changes of dna methylation level and spatial arrangement of primordial germ cells in embryonic day 15 to embryonic day 28 pig embryos. | the mammalian germline is generally assumed to undergo extensive epigenetic reprogramming during embryonic development, including a nearly complete erasure of dna methylation. this assumption does, however, to large degree rely on data from mouse, and despite a well-grounded picture the general nature of these data needs to be validated by investigations of other mammalian species. this study represents such a contribution in the examination of the germline in the domestic pig (sus scrofa). semi ... | 2011 | 21293033 |
| peptide vaccine candidates against classical swine fever virus: t cell and neutralizing antibody responses of dendrimers displaying e2 and ns2-3 epitopes. | three peptide-based systems integrating b and t antigenic sites of csfv and displaying the b epitopes in fourfold presentation have been designed and produced, and shown to bring about significant enhancements in immunogenicity over the peptides in monomeric form. of the different strategies tested for producing the dendrimeric constructs, stepwise spps using 3,6-dioxaoctanoic acid as flexible, peg-like spacer units at the branching points is clearly advantageous, in particular over ligation in ... | 2011 | 20812373 |
| new piophilid in town: the first palaearctic record of piophila megastigmata and its coexistence with piophila casei in central spain. | the genus piophila fallén (diptera: piophilidae) is known from only two species: piophila casei (l.) is a major pest, a cosmopolitan species and is commonly used as a forensic indicator, whereas piophila megastigmata mcalpine has until now only been recorded in a natural environment in south africa. the present work reports the first occurrence of p. megastigmata in the palaearctic region from specimens collected by carrion-baited traps throughout different natural habitats of central spain. fur ... | 2011 | 20819151 |
| video: transcervical videoscopic esophageal dissection in minimally invasive esophagectomy. | minimally invasive esophagectomy (mie) may involve video-assisted thoracoscopic surgery (vats) for mediastinal esophageal dissection. usually, vats requires single-lung ventilation and has associated cardiopulmonary morbidity [1-3]. alternatively, transhiatal dissection can be performed, although its complications include vocal cord palsy [4], cardiac arrythmias [5], and increased bleeding [5, 6], the latter associated with mortality after esophagectomy [2]. therefore, the feasibility of mie usi ... | 2011 | 20844900 |
| comparison of the substrate kinetics of pig cyp3a29 with pig liver microsomes and human cyp3a4. | cyp (cytochrome p450) 3a29 in pigs could be an important candidate gene responsible for xenobiotic metabolism, similar to cyp3a4 in humans. accordingly, the tissue expression of cyp3a29 mrna in domestic pigs has been determined by a real-time pcr. the enzymatic properties of cyp3a29, cyp3a4 and plm (pig liver microsomes) were compared by kinetic analysis of tst (testosterone) 6β-hydroxylation and nif (nifedipine) oxidation. cyp3a29 mrna was highly expressed in the liver and small intestines of d ... | 2011 | 20863320 |
| inside out: modern imaging techniques to reveal animal anatomy. | animal anatomy has traditionally relied on detailed dissections to produce anatomical illustrations, but modern imaging modalities, such as mri and ct, now represent an enormous resource that allows for fast non-invasive visualizations of animal anatomy in living animals. these modalities also allow for creation of three-dimensional representations that can be of considerable value in the dissemination of anatomical studies. in this methodological review, we present our experiences using mri, ct ... | 2011 | 21445356 |
| dynamic dissociating homo-oligomers and the control of protein function. | homo-oligomeric protein assemblies are known to participate in dynamic association/disassociation equilibria under native conditions, thus creating an equilibrium of assembly states. such quaternary structure equilibria may be influenced in a physiologically significant manner either by covalent modification or by the non-covalent binding of ligands. this review follows the evolution of ideas about homo-oligomeric equilibria through the 20th and into the 21st centuries and the relationship of th ... | 2011 | 22182754 |
| heritable targeted inactivation of myostatin gene in yellow catfish (pelteobagrus fulvidraco) using engineered zinc finger nucleases. | yellow catfish (pelteobagrus fulvidraco) is one of the most important freshwater aquaculture species in china. however, its small size and lower meat yield limit its edible value. myostatin (mstn) is a negative regulator of mammalian muscle growth. but, the function of mstn in fish remains elusive. to explore roles of mstn gene in fish growth and create a strain of yellow catfish with high amount of muscle mass, we performed targeted disruption of mstn in yellow catfish using engineered zinc-fin ... | 2011 | 22194943 |
| a genomic approach to examine the complex evolution of laurasiatherian mammals. | recent phylogenomic studies have failed to conclusively resolve certain branches of the placental mammalian tree, despite the evolutionary analysis of genomic data from 32 species. previous analyses of single genes and retroposon insertion data yielded support for different phylogenetic scenarios for the most basal divergences. the results indicated that some mammalian divergences were best interpreted not as a single bifurcating tree, but as an evolutionary network. in these studies the relatio ... | 2011 | 22164244 |
| optical properties of in situ eye lenses measured with x-ray talbot interferometry: a novel measure of growth processes. | the lens, a major optical component of the eye, has a gradient refractive index, which is required to provide sufficient refractive power and image quality. the refractive index variations across the lens are dependent on the distributions and concentrations of the varying protein classes. in this study, we present the first measurements of the refractive index in the in situ eye lens from five species using a specially constructed x-ray talbot grating interferometer. the measurements have been ... | 2011 | 21949870 |
| performance of a novel dual-electrode bipolar radiofrequency ablation device: a chronic porcine study. | : over recent years, a variety of energy sources, including bipolar radiofrequency, have been used to replace the traditional incisions of the cox-maze procedure for the surgical treatment of atrial fibrillation (af). the purpose of this study was to evaluate the safety and efficacy of a novel dual-electrode bipolar radiofrequency ablation device synergy (atricure, inc., cincinnati, oh usa) for af in a chronic porcine model. | 2011 | 22437797 |
| δ18o values of sus scrofa blood water and bone phosphate; a marked discrepancy between domestic and wild specimens. | δ¹⁸o analyses of water in the blood of domestic and wild pigs indicated that large isotopic differences exist between domestic and wild specimens of the same species (sus scrofa) living in the same area. similar isotopic differences are found between the δ¹⁸o(po₄³⁻) values of bones from the two groups of animals. when δ¹⁸o values obtained from recent wild boar bones are introduced in the equation of the isotopic scale determined for domestic pigs, totally unreliable δ¹⁸o values of local meteoric ... | 2011 | 22468328 |
| early myocardial injury is an integral component of experimental acute liver failure - a study in two porcine models. | there is accumulating clinical evidence that acute liver failure may be regularly associated with myocardial injury. to test this hypothesis in a standardized experimental setting, we used two porcine models of alf. | 2011 | 22291759 |
| no apparent effect of an experimental narrow confinement on heart activity and cortisol in domestic pigs. | the effects of continuing spatial restraint were examined in domestic pigs. for this purpose, the animals (german landrace barrows) were housed individually in metabolic cages (12 animals) and, as controls, in single pens (six animals). in six replications with two experimental animals and one control animal, we collected saliva each morning (0730 h) for the cortisol analysis, recorded the behaviour and the heart beat for 3 h/day (0800 to 0900 h, 1100 to 1200 h, 1400 to 1500 h). each replication ... | 2011 | 22445410 |
| trapping as an alternative method of eradicating classical swine fever in a wild boar population in bulgaria. | between august and november 2009, eight cases of classical swine fever (csf) occurred in young wild boar in a 25-km2 oak forest3 km south of the river danube in the north-eastern part of bulgaria. the wild boar population within the affected area was estimated to be 156 animals, or approximately six boar per km2. to control and eradicate the disease, and in addition to vaccination and hunting, trapping was used to reduce the boar population to below two animals per km2. in total, 124 wild boar w ... | 2011 | 22435201 |
| cutaneous challenge with chemical warfare agents in the skh-1 hairless mouse (ii): effects of some currently used skin decontaminants (rsdl and fuller's earth) against liquid sulphur mustard and vx exposure. | using the hairless mouse screening model presented in the companion paper(1) the aim of this study was to assess two skin decontaminating systems: fuller's earth (fe) and reactive skin decontamination lotion (rsdl) against two extremely toxic chemical warfare agents that represent a special percutaneous hazard, sulphur mustard (sm) and o-ethyl-s-(2[di-isopropylamino]ethyl)methyl-phosphonothioate (vx). five minutes after being exposed on the back to either 2 µl of neat sulphur mustard or 50 µg.kg ... | 2011 | 20534641 |
| divergence time estimates of east asian and european pigs based on multiple near complete mitochondrial dna sequences. | the time since the divergence of european and east asian domestic pigs and wild boars has been estimated in several phylogenetic analyses, generally based on partial mitochondrial sequences or on a small number of complete mtdna sequences. in the present study, we obtained a refined estimate of this divergence time based on a set of 32 near-complete mtdna sequences from wild and domestic pigs of european and asian types, including 14 new and 18 previously published sequences. a weighted average ... | 2011 | 20477794 |
| protective effect of nac preconditioning against ischemia-reperfusion injury in piglet small bowel transplantation: effects on plasma tnf, il-8, hyaluronic acid, and no. | ischemia-reperfusion (i/r) injury is one of the main factors affecting the function and structure of small bowel transplantation (sbt), by generation of proinflammatory mediators such as reactive oxygen species, reactive nitrogen species, cytokines, and endotoxin. experimental data have demonstrated that n-acetylcysteine (nac) attenuates intestinal i/r injury. the objective of this study was to determine the effect of nac preconditioning on the sbt-i/r induced inflammatory cascade, with particul ... | 2011 | 20036383 |
| effects of sodium selenite and amiloride on calvarial calcification in closing small cranial defects. | fresh autogenous bone graft is the most preferred osteoplastic material, whether the purpose is cosmetic, psychological, or for the protection of the brain. these grafts are not rejected and do not react immunologically. the aim of this study was to evaluate the efficacy of autogenous fat rolled with bone dust derived from the bur hole in closing small cranial defects. additionally, the authors examined the morphological and biochemical effects of na selenite and amiloride on calvarial calcifica ... | 2011 | 20672896 |
| durability of the self-approximating translumenal access technique (stat) for potential use in natural orifice translumenal surgery (notes). | the self-approximating translumenal access technique (stat) has been shown to provide a safe and reliable means of abdominal access for natural orifice translumenal endoscopic surgery (notes). however, the feasibility of using stat for translumenal organ resection is unknown. this study aimed to evaluate the technical performance of organ resection using stat, the integrity of the stat gastric tunnel after organ resection, and the postoperative morbidity of organ resection using stat. | 2011 | 20725749 |
| rapid intradermal delivery of liquid formulations using a hollow microstructured array. | the purpose of this work is to demonstrate rapid intradermal delivery of up to 1.5 ml of formulation using a hollow microneedle delivery device designed for self-application. | 2011 | 20582455 |
| mitochondrial dna diversity in wild boar from the primorsky krai region (east russia). | we have studied the cytochrome b gene and control region dna variability in 14 wild boars from the primorsky region, in the far east corner of russia. variability was low (π = 0.00248 overall) compared with the usual estimates in these loci, indicating that this is a rather closed population. seven haplotypes were found, and one was identical to a chinese wild boar. phylogeographically, the sequences clustered among several asian clades, primarily chinese domestic pigs and japanese and chinese w ... | 2011 | 20477807 |
| phylogenetic analysis of recent classical swine fever virus (csfv) isolates from assam, india. | classical swine fever (csf), a highly contagious viral disease of pigs, is endemic in india. as there is no information concerning the accurate genetic typing of classical swine fever virus (csfv) isolates in india, 16 csf viruses isolated during 2005-2007 from domestic pigs in different districts of assam were typed in 5' utr (150 nucleotides). to confirm the genetic typing results and to study the genetic variability, selected viruses were also analyzed in e2 (190 nt) and ns5b gene (409 nt) re ... | 2011 | 19896713 |
| development of a microsatellite-based method for the differentiation of european wild boar (sus scrofa scrofa) from domestic pig breeds (sus scrofa domestica) in food. | twenty microsatellites (simple sequence repeats, ssr) were used to discriminate wild boar from domestic pig and to identify mixtures of the two. reference groups of wild boar and pig samples were collected from the uk and europe for genetic assignment tests. bayesian analysis of populations software (baps) gave 100% correct assignment for blind wild boar and pig samples and correctly identified mixed samples. dna was extracted from 12 commercial food samples (11 labeled as containing wild boar) ... | 2012 | 22409233 |
| analysis of biomarkers characteristic of porcine liver injury--from biomolecular logic gates to an animal model. | a biocatalytic cascade for the analysis of the simultaneous increase in the concentration of two biomarkers characteristic of liver injury (alanine transaminase, alt, and lactate dehydrogenase, ldh) was tested on real samples acquired from an animal model (domestic pigs, sus scrofa domesticus) suffering from traumatic liver injury. a two-step reaction biocatalyzed in the presence of both enzyme-biomarkers resulted in the oxidation of nadh followed by optical absorbance measurements. a simple qua ... | 2012 | 22407106 |