Publications

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glycerol monolaurate nanocapsules for biomedical applications: in vitro toxicological studies.the glycerol monolaurate (gml) is a surfactant used in the food industry and has potent antimicrobial activity against many microorganisms; however, the use of gml is not expanded due its high melting point and poor solubility in water. the aim of the study was to produce, characterize, and evaluate in vitro the cytotoxicity of gml and gml nanocapsules. the gml nanocapsules were produced and characterized by a mean diameter, zeta potential, and polydispersity index. the cytotoxicity was evaluate ...201931079199
characterization of glucose-stimulated insulin release protocols in african green monkeys (chlorocebus aethiops).management of diabetes remains a major health and economic challenge, demanding test systems in which to develop new therapies. these studies assessed different methodologies for determining glucose tolerance in green monkeys.201930357856
low-ph endocytic entry of the porcine alphaherpesvirus pseudorabies virus.the alphaherpesvirus pseudorabies virus (prv) is the causative agent of pseudorabies, a disease of great economic and welfare importance in swine. other alphaherpesviruses, including herpes simplex virus (hsv), utilize low-ph-mediated endocytosis to enter a subset of cell types. we investigated whether prv used this entry pathway in multiple laboratory model cell lines. inhibition of receptor-mediated endocytosis by treatment with hypertonic medium prevented prv entry. prv entry into several cel ...201930355685
schistosoma mansoni infection in human and nonhuman primates in selected areas of oromia regional state, ethiopia.the transmission of schistosomiasis, caused by trematodes of the genus schistosoma, relies on freshwater snails that act as an intermediate host while human and other mammalian act as the definitive hosts. many non-human primates (nhps) such as chlorocebus aethiops (vervet) and papio anubis (baboon) are reported to be infected with schistosoma mansoni in ethiopia, but the role they play in parasite maintenance and transmission is still not clear. the objective of this study was, therefore, to de ...201930280709
an azine-based carbothioamide chemosensor for selective and sensitive turn-on-off sequential detection of zn(ii) and h2po4-, live cell imaging and inhibit logic gate.hydrazino-carbothioamide, 1, serves as a turn-on fluorescent chemosensor to zn2+ and the mixture shows green emission (λem 492) in presence of large number of ions with impressive limit of detection (lod), 0.59 nm. intense fluorescence of zn-complex is selectively turn-off upon adding h2po4- only to the limit of detection, 26 μm, while other anions remain silent. the sensing mechanism of 1 is established by suppressing the esipt of 1 and complexation with zn2+ (chef) is supported by job's plot, ...201930227347
myrislignan exhibits activities against toxoplasma gondii rh strain by triggering mitochondrial dysfunction.toxoplasma gondii is a widespread obligatory parasitic protozoon that infects nearly all warm-blooded animals and causes toxoplasmosis. however, the current treatments for toxoplasmosis are limited by severe side effects. myrislignan is a natural product from myristica fragrans houtt with wide pharmacological activities. in the current study, we tested the anti-t. gondii activity of myrislignan both in vitro and in vivo and explored its potential mechanism of action. the cytotoxicity of myrislig ...201931620108
integrin αvβ3 enhances replication of porcine epidemic diarrhea virus on vero e6 and porcine intestinal epithelial cells.the entry mechanism of porcine epidemic diarrhea virus (pedv) remains unclear, especially the virus receptor. our previous study revealed a potential correlation between integrin αvβ3 and pedv infection. in the current study, the effect of overexpression, silencing, antibody inhibition, and co-expression with porcine aminopeptidase n (papn) of integrin αvβ3 on pedv infection was investigated and analyzed in african green monkey vero e6 cells and porcine intestinal epithelial cells (iecs) using t ...201931585640
in vitro anticancer, anti-inflammatory, and antioxidant potentials of ephedra aphylla.the goal of our study is to test whether a naturally occurring plant, ephedra aphylla, will show antiproliferative ability against tested cell lines and to test its anti-inflammatory and antioxidative potentials.201930488855
a nonhuman primate model of early alzheimer's disease pathologic change: implications for disease pathogenesis.nonhuman primates may serve as excellent models of sporadic age-associated brain β-amyloid deposition and alzheimer's disease pathologic changes. we examined whether a vervet nonhuman primate model recapitulated pathologic, physiologic, and behavioral features of early alzheimer's disease.201930467082
triterpene derivatives as relevant scaffold for new antibiofilm drugs.new medicines for the treatment of bacterial biofilm formation are required. for thisreason, this study shows the in vitro activity of betulinic acid (ba), ursolic acid (ua) and their twentyderivatives against planktonic and biofilm cells (gram-positive bacterial pathogens: enterococcusfaecalis, <i>staphylococcus aureus</i> and <i>staphylococcus epidermidis</i>). we evaluated the antibiofilm activity(through the crystal violet method), as well as the antibacterial activity via absorbance (od<sub ...201930754716
nonketotic hyperglycinemia in captive-bred vervet monkeys (chlorocebus aethiops) with cataracts.nonketotic hyperglycinemia (nkh) is a rare metabolic disorder that is characterized by high levels of glycine in plasma and cerebrospinal fluid in humans. in this study, total congenital cataract captive-bred vervet monkeys (chlorocebus aethiops) that are hyperglycinemic were screened to identify mutations in bola type 3 (bola3), glutaredoxin 5 (glrx5), and lipoate synthase (lias) genes.201930724368
erk-independent african green monkey pluripotent stem cells in a putative chimera-competent state.generating human organs inside interspecies chimeras might one day produce patient-specific organs for clinical applications, but further advances in identifying human chimera-competent pluripotent stem (ps) cells are needed. moreover, the potential for human ps cells to contribute to the brains in human-animal chimeras raises ethical questions. the use of non-human primate (nhp) chimera-competent ps cells would allow one to test interspecies organogenesis strategies while also bypassing such et ...201930660369
author correction: macrophage-associated wound healing contributes to african green monkey siv pathogenesis control.an amendment to this paper has been published and can be accessed via a link at the top of the paper.201931836755
a preliminary survey of medium and large-sized mammals from lebu natural protected forest, southwest showa, ethiopia.this study was conducted to determine the species composition and diversity of medium and large-sized mammals from lebu natural protected forest, ethiopia. surveys were conducted to record mammals through direct observation and indirect evidence from three habitat types, namely: natural forest, bushland, and riverine forest. a total of 15 mammalian species were recorded. the species recorded were papio anubis, chlorocebus aethiops, tragelaphus scriptus, canis aureus, crocuta crocuta, panthera pa ...201931832163
decreased antibiotic susceptibility driven by global remodeling of the klebsiella pneumoniae proteome.bacteria can circumvent the effect of antibiotics by transitioning to a poorly understood physiological state that does not involve conventional genetic elements of resistance. here we examine antibiotic susceptibility with a class a β-lactamase+ invasive strain of klebsiella pneumoniae that was isolated from a lethal outbreak within laboratory colonies of chlorocebus aethiops sabaeus monkeys. bacterial responses to the ribosomal synthesis inhibitors streptomycin and doxycycline resulted in dist ...201930617156
natural variation in fetal cortisol exposure is associated with neonatal body mass in captive vervet monkeys (chlorocebus aethiops).poor maternal condition during gestation is commonly associated with impaired fetal growth in humans and other animals. although elevated maternal glucocorticoids (gcs) are often implicated as the mechanism of intrauterine growth stunting, the direct contribution of maternal gcs remains unclear because enzymatic conversion of gcs at the placenta may limit the ability of maternal hormones to reach the fetus. further, because previous studies on gestational stress have often employed synthetic gcs ...201930604879
hemagglutinin head-specific responses dominate over stem-specific responses following prime boost with mismatched vaccines.broadly neutralizing abs targeting the ha stem can provide broad protection against different influenza subtypes, raising the question of how best to elicit such abs. we have previously demonstrated that vaccination with pandemic live-attenuated influenza vaccine (plaiv) establishes immune memory for ha head-specific abs. here, we determine the extent to which matched versus mismatched laiv-inactivated subunit vaccine (iiv) prime-boost vaccination elicits stem-specific memory b cells and abs. we ...201931723058
high-fat diet exacerbates siv pathogenesis and accelerates disease progression.consuming a high-fat diet (hfd) is a risk factor for obesity and diabetes; both of these diseases are also associated with systemic inflammation, similar to hiv infection. a hfd induces intestinal dysbiosis and impairs liver function and coagulation, with a potential negative impact on hiv/siv pathogenesis. we administered a hfd rich in saturated fats and cholesterol to nonpathogenic (african green monkeys) and pathogenic (pigtailed macaques) siv hosts. the hfd had a negative impact on siv disea ...201931710311
macrophage-associated wound healing contributes to african green monkey siv pathogenesis control.natural hosts of simian immunodeficiency virus (siv) avoid aids despite lifelong infection. here, we examined how this outcome is achieved by comparing a natural siv host, african green monkey (agm) to an aids susceptible species, rhesus macaque (rm). to asses gene expression profiles from acutely siv infected agms and rms, we developed a systems biology approach termed conserved gene signature analysis (cgsa), which compared rna sequencing data from rectal agm and rm tissues to various other sp ...201931704931
behavioral, pharmacokinetic, and cardiovascular evaluation of candidate oximes in african green monkeys.oxime reactivators are critical antidotes after organophosphate pesticide or nerve agent poisoning, directly restoring the function of inhibited acetylcholinesterase. in the continuing search for more broad-spectrum acetylcholinesterase reactivators, this study evaluated one of the leading next-generation oxime reactivators: methoxime, (1,1'-trimethylene bis[4-(hydroxyimino)methyl]pyridinium dichloride (mmb-4). the pharmacokinetics of both salts of mmb-4 (dichloride [2cl] and dimethanesulphonate ...201929879849
signature microrna expression profile is associated with spontaneous hypertension in african green monkey.chlorocebus aethiops sabaeus, the african green monkey (agm), has been proved to exhibit renal vascular remodeling and spontaneous hypertension. however, little is known about the roles of micrornas (mirnas) in this process.using small rna deep sequencing, we compared the plasma mirna expression patterns between hypertensive (ht) agms and normotensive (nt) agms. expression of mirnas (mir-122, mir-339, mir-296-5p) was validated independently in plasma samples from 10 ht agms and 10 nt agms (fold ...201929787292
antimicrobial activity, antioxidant potential, cytotoxicity and phytochemical profiling of four plants locally used against skin diseases.although orthodox medications are available for skin diseases, expensive dermatological services have necessitated the use of medicinal plants as a cheaper alternative. this study evaluated the pharmacological and phytochemical profiles of four medicinal plants (drimia sanguinea, elephantorrhiza elephantina, helichrysum paronychioides, and senecio longiflorus) used for treating skin diseases. petroleum ether and 50% methanol extracts of the plants were screened for antimicrobial activity against ...201931540194
endogenous specialized proresolving mediator profiles in a novel experimental model of lymphatic obstruction and intestinal inflammation in african green monkeys.changes in the intestinal lymphatic vascular system, such as lymphatic obstruction, are characteristic features of inflammatory bowel diseases. the lymphatic vasculature forms a conduit to enable resolution of inflammation; this process is driven by specialized endogenous proresolving mediators (spms). to evaluate contributions of lymphatic obstruction to intestinal inflammation and to study profiles of spms, we generated a novel animal model of lymphatic obstruction using african green monkeys. ...201931547920
signature microrna expression profile is associated with lipid metabolism in african green monkey.non-human primates (nhps) are important models of medical research on obesity and cardiovascular diseases. as two of the most commonly used nhps, cynomolgus macaque (cm) and african green monkey (agm) own different capacities in lipid metabolism of which the mechanism is unknown. this study investigated the expression profiles of lipid metabolism-related micrornas (mirnas) in cm and agm and their possible roles in controlling lipid metabolism-related gene expression.201930819205
application of benzonase in preparation of decellularized lamellar porcine corneal stroma for lamellar keratoplasty.this study was to develop anovel and efficient method using endonuclease (benzonase) to preparedecellularized lamellar porcine corneal stroma (dlpcs). the dlpcs was preparedfrom native lamellar porcine corneal stroma (nlpcs) and was treated with 1000 u/ml benzonase for 5hours. we conducted the following measurements and animal transplantation tocompare dlpcs and nlpcs. the residual dna was decreased significantly from 367.13 ± 19.96 ng/mg to 15.41 ± 0.65 ng/mg after treatment of benzonase by the ...201931330094
differential susceptibility & replication potential of vero e6, bhk-21, rd, a-549, c6/36 cells & aedes aegypti mosquitoes to three strains of chikungunya virus.chikungunya virus (chikv), a mosquito-borne arthritogenic virus causes infections ranging from febrile illness to debilitating polyarthralgia in humans. re-emergence of the virus has affected millions of people in africa and asia since 2004. during the outbreak, a new lineage of the virus has evolved as an adaptation for enhanced replication and transmission by aedes albopictus mosquito. a study was designed to compare the susceptibility of four vertebrate cell lines, namely vero e6 (african gre ...201931496530
acute respiratory distress in aged, sars-cov-2 infected african green monkeys but not rhesus macaques.sars-cov-2 induces a wide range of disease severity ranging from asymptomatic infection, to a life-threating illness, particularly in the elderly and persons with comorbid conditions. among those persons with serious covid-19 disease, acute respiratory distress syndrome (ards) is a common and often fatal presentation. animal models of sars-cov-2 infection that manifest severe disease are needed to investigate the pathogenesis of covid-19 induced ards and evaluate therapeutic strategies. here we ...202033171111
synthesis of novel cyanine dyes as antitumor agents.in this study, some novel cyanine dyes, 1, 3, and 5-15, were synthesized by a one-pot step reaction of pyridinium salts 2 and/or 4 with benzenaminium salt 1. n-{[1-chloro-3,4-dihydronaphthalen-2-yl)methylene]benzenaminium} chloride 1 was obtained by the reaction of α-tetralone with vilsmeier-haack reagent, followed by a mixture of an equimolar ratio of anilin/ethanol (1:1). all new cyanine dyes were evaluated in vitro for their anticancer activity against two cell lines, that is, hepg2 (human he ...202033169870
evaluation of host systems for efficient isolation and propagation of duck tembusu virus.several phylogenetic clusters of duck tembusu virus (dtmuv) that caused outbreaks in ducks in asia have been identified since its emergence in 2010, highlighting the need for an efficient host system that can support isolation of all circulating phylogenetic clusters of dtmuv. in this study, various host systems including different avian embryonated eggs (duck and chicken) and cell cultures (primary duck embryo fibroblast (def), primary chicken embryo fibroblast (cef), baby hamster kidney (bhk-2 ...202033146547
characterization of a novel peptide from pathogenic leptospira and its cytotoxic effect.leptospirosis is a zoonotic infectious disease caused by pathogenic leptospira species. virulence proteins have been shown to be key determinants of the pathogenesis of pathogenic leptospira. a specific peptide at a mass-to-charge ratio of 7000 da was identified in leptospira whole cells using matrix-assisted laser/desorption ionization time-of-flight (maldi-tof) mass spectrometry. this peptide was specifically present in pathogenic leptospira and in clinical isolates. we report here the charact ...202033142957
no evidence for sylvatic cycles of chikungunya, dengue and zika viruses in african green monkeys (chlorocebus aethiops sabaeus) on st. kitts, west indies.dengue, chikungunya and zika viruses (denv, chikv and zikv) are transmitted in sylvatic transmission cycles between non-human primates and forest (sylvan) mosquitoes in africa and asia. it remains unclear if sylvatic cycles exist or could establish themselves elsewhere and contribute to the epidemiology of these diseases. the caribbean island of st. kitts has a large african green monkey (agm) (chlorocebus aethiops sabaeus) population and is therefore ideally suited to investigate sylvatic cycle ...202033126907
a parainfluenza virus vector expressing the respiratory syncytial virus (rsv) prefusion f protein is more effective than rsv for boosting a primary immunization with rsv.live-attenuated pediatric vaccines for intranasal administration are being developed for human respiratory syncytial virus (rsv), an important worldwide pediatric respiratory pathogen that lacks a licensed vaccine or suitable antiviral drug. we evaluated a prime-boost strategy in which primary immunization with rsv was boosted by secondary immunization with rsv or with a chimeric bovine/human parainfluenza virus type 3 (rb/hpiv3) vector expressing the rsv fusion f protein. the vector-expressed f ...202033115876
phytochemical screening, antibacterial, antifungal, antiviral, cytotoxic, and anti-quorum-sensing properties of teucrium polium l. aerial parts methanolic extract.the chemical profile of teucrium polium l. (t. polium) methanolic extract was tested using liquid chromatography coupled with high resolution mass spectrometry (hr-lcms). disc diffusion and microdilution assays were used for the antimicrobial activities. coxsackievirus b-3 (cvb3) and herpes simplex virus type 2 (hsv-2) were used for the antiviral activities. chromobacterium violaceum (atcc 12472 and cv026) and pseudomonas aeruginosa pao1 were used as starter strains for the anti-quorum sensing t ...202033114026
the protein translocation defect of mct8l291r is rescued by sodium phenylbutyrate.the monocarboxylate transporter 8 (mct8; slc16a2) is a specific transporter for thyroid hormones. mct8 deficiency, formerly known as the allan-herndon-dudley syndrome, is a rare genetic disease that leads to neurological impairments and muscle weakness. current experimental treatment options rely on thyromimetic agonists that do not depend on mct8 for cellular uptake. another approach comes from studies with the chemical chaperone sodium phenylbutyrate (napb), which was able to stabilize mct8 mu ...202033088796
identification of a novel simian immunodeficiency virus-infected african green monkey (chlorocebus tantalus) confirms that tantalus monkeys in cameroon are infected with a mosaic sivagm lineage.in this study we report on the identification of a simian immunodeficiency virus (siv) infecting a chlorocebus tantalus from cameroon. the isolate, sivagmtan-ca1, was molecularly characterized by sequencing partial genome (∼4,000 bp) using the conventional sanger method and the oxford nanopore technology (ont). in pol and gp41/nef sivagmtan-ca1 clusters with sivagmsab infecting chlorocebus sabaeus from west africa, whereas in env-gp120 it clusters with sivagmtan infecting c. tantalus from centra ...202031547667
the use of large-particle aerosol exposure to nipah virus to mimic human neurological disease manifestations in the african green monkey.nipah virus (niv) is an emerging virus associated with outbreaks of acute respiratory disease and encephalitis. to develop a neurological model for niv infection, we exposed 6 adult african green monkeys to a large-particle (approximately 12 μm) aerosol containing niv (malaysian isolate). brain magnetic resonance images were obtained at baseline, every 3 days after exposure for 2 weeks, and then weekly until week 8 after exposure. four of six animals showed abnormalities reminiscent of human dis ...202031687756
broad diversity of simian immunodeficiency virus infecting chlorocebus species (african green monkey) and evidence of cross-species infection in papio anubis (olive baboon) in kenya.simian immunodeficiency virus (siv) naturally infects african non-human primates (nhps) and poses a threat of transmission to humans through hunting and consumption of monkeys as bushmeat. this study investigated the as of yet unknown molecular diversity of siv in free-ranging chlorocebus species (african green monkeys-agms) and papio anubis (olive baboons) within mombasa, kisumu and naivasha urban centres in kenya.202032030774
carfentanil toxicity in the african green monkey: therapeutic efficacy of naloxone.carfentanil is an ultra-potent opioid with an analgesic potency 10,000 times that of morphine but has received little scientific investigation. three experiments were conducted to evaluate the toxicity of carfentanil and the efficacy of naloxone in adult male african green monkeys. the first experiment determined the ed50 (found to be 0.71 μg/kg) of subcutaneous carfentanil for inducing bradypnea and/or loss of posture. experiment 2 attempted to establish the ed50 of naloxone for rapidly reversi ...202032070766
nanofibrous silica microparticles/polymer hybrid aerogels for sustained delivery of poorly water-soluble camptothecin.the surface functionalization of nanoporous silica materials with chemical agents opens up numerous possibilities, including improvement in the materials' ability to carry high payloads of drugs. in this study, kcc-1 nanofibrous silica microparticles are functionalized with methyl groups and then combined with poly(vinyl alcohol) (pva) and poly(acrylic acid) (paa) to produce hybrid aerogels that can deliver a poorly water-soluble anticancer drug. the synthetic steps involve freeze-drying a polym ...202032036118
african green monkeys avoid siv disease progression by preventing intestinal dysfunction and maintaining mucosal barrier integrity.unlike hiv infection, siv infection is generally nonpathogenic in natural hosts, such as african green monkeys (agms), despite life-long high viral replication. lack of disease progression was reportedly based on the ability of siv-infected agms to prevent gut dysfunction, avoiding microbial translocation and the associated systemic immune activation and chronic inflammation. yet, the maintenance of gut integrity has never been documented, and the mechanism(s) by which gut integrity is preserved ...202032119719
a codon-pair deoptimized live-attenuated vaccine against respiratory syncytial virus is immunogenic and efficacious in non-human primates.despite a critical need for a respiratory syncytial virus (rsv) vaccine and decades of development efforts, a vaccine to protect infants, elderly, and other at-risk populations from rsv infection remains elusive. we have previously generated a new, live-attenuated vaccine candidate against rsv using rational, computer-aided gene design and chemical synthesis through a process termed viral gene "deoptimization." in this study, we assessed the attenuation, immunogenicity, and efficacy of this synt ...202032107060
human immunodeficiency virus type-1 tat protein induces secretory leukocyte protease inhibitor expression in african green monkey but not human cells.african monkeys are resistant to hiv-1 infection due to intrinsic restriction mechanisms found in their cells. however, although they can be infected by monkey-adapted modified hiv-1 particles that are designed to overcome known restriction factors, virus numbers drop to undetectable levels in immunocompetent animals. these results indicate the possibility of the presence of yet unidentified factor(s) that restrict hiv-1 in old-world monkey (owm) cells after integration of the viral genome into ...202031925640
the influence of tobacco smoke/nicotine on cyp2a expression in human and african green monkey lungs.cyp2a enzymes metabolically inactivate nicotine and activate tobacco-derived procarcinogens [e.g., 4-[methylnitrosamino]-1-(3-pyridyl)-1-butanone]. smoking decreases nicotine clearance, and chronic nicotine reduces hepatic cyp2a activity. however, little is known about the impact of smoking or nicotine on the expression of cyp2a in the lung. we investigated 1) the levels of human lung cyp2a mrna in smokers versus nonsmokers and 2) the impact of daily nicotine treatment on lung cyp2a protein leve ...202033055223
a novel field-deployable method for sequencing and analyses of henipavirus genomes from complex samples on the minion platform.viruses in the genus henipavirus encompass 2 highly pathogenic emerging zoonotic pathogens, hendra virus (hev) and nipah virus (niv). despite the impact on human health, there is currently limited full-genome sequence information available for henipaviruses. this lack of full-length genomes hampers our ability to understand the molecular drivers of henipavirus emergence. furthermore, rapidly deployable viral genome sequencing can be an integral part of outbreak response and epidemiological inves ...202031784761
quantitative proteomic analysis reveals antiviral and anti-inflammatory effects of puerarin in piglets infected with porcine epidemic diarrhea virus.porcine epidemic diarrhea virus (pedv) has caused enormous economic losses to the swine industry worldwide in recent years. puerarin (pr), a major isoflavonoid isolated from the chinese herb gegen, possesses many pharmacological activities, including anti-inflammatory, and anti-viral activities. this study was conducted with both pedv-infected african green monkey kidney cells (vero) and neonatal pigs to determine the effect of pr on pedv infection and to elucidate the underlying mechanisms by u ...202032174911
virome diversity analysis reveals novel enteroviruses and a human picobirnavirus in stool samples from african green monkeys with diarrhea.it is important to identify viruses in animals because most infectious diseases in humans are caused by viruses of zoonotic origin. african green monkey is a widely used non-human primate model in biomedical investigations. in this study, total rnas were extracted from stool samples of 10 african green monkeys with diarrhea. high-throughput sequencing was used to characterize viromes. pcr and sanger sequencing were used to determine the full genome sequences. great viral diversity was observed. ...202032165243
pharmacological characterization of the novel and selective α7 nicotinic acetylcholine receptor-positive allosteric modulator bnc375.treatments for cognitive deficits associated with central nervous system (cns) disorders such as alzheimer disease and schizophrenia remain significant unmet medical needs that incur substantial pressure on the health care system. the α7 nicotinic acetylcholine receptor (nachr) has garnered substantial attention as a target for cognitive deficits based on receptor localization, robust preclinical effects, genetics implicating its involvement in cognitive disorders, and encouraging, albeit mixed, ...202032094294
evaluation of acute oral toxicity, embryotoxicity and cytotoxicity of the polar fraction of parkinsonia aculeata aerial parts extract.ethnopharmacobotanical information reports that parkinsonia aculeata infusion is used to control diabetes-related complications and dyslipidemia. however, few studies are reported on the safe use of this species. the aim of this study is to evaluate the acute toxicity, embryotoxicity and cytotoxicity of a polar fraction obtained from hydroethanolic extract of p. aculeata (pfrhepa). for the acute toxicity test, we considered the up and down method which the guidelines are described by the organiz ...202032440335
effect of delaying treatment on efficacy of ciprofloxacin and levofloxacin in the african green monkey model of pneumonic plague.ciprofloxacin and levofloxacin, 2 fluoroquinolone antimicrobials, are ≥90% effective for the treatment of inhalational plague when administered within 2-6 hours of fever onset in african green monkeys (agm). based on data in the agm model, these antimicrobials were approved under the food and drug administration's animal efficacy rule. however, that data did not address the issue of how long treatment with these antimicrobials would remain effective after fever onset.202032435805
nogo receptor decoy promotes recovery and corticospinal growth in non-human primate spinal cord injury.after cns trauma such as spinal cord injury, the ability of surviving neural elements to sprout axons, reorganize neural networks and support recovery of function is severely restricted, contributing to chronic neurological deficits. among limitations on neural recovery are myelin-associated inhibitors functioning as ligands for neuronal nogo receptor 1 (ngr1). a soluble decoy (ngr1-fc, axer-204) blocks these ligands and provides a means to promote recovery of function in multiple preclinical ro ...202032375169
comparative characterization of flavivirus production in two cell lines: human hepatoma-derived huh7.5.1-8 and african green monkey kidney-derived vero.the flaviviridae is a family of enveloped viruses with a positive-sense single-stranded rna genome. it contains many viruses that threaten human health, such as japanese encephalitis virus (jev) and yellow fever virus (yfv) of the genus flavivirus as well as hepatitis c virus of the genus hepacivirus. cell culture systems highly permissive for the flaviviridae viruses are very useful for their isolation, propagation, and diagnosis, an understanding of their biology, and the development of vaccin ...202032330205
primate model of chronic retinal neovascularization and vascular leakage.the purpose of this study was to characterize and develop a primate model of chronic retinal neovascularization and vascular leakage that can be employed to assess efficacy of experimental therapeutics targeting retinal ischemic and neovascular diseases. african green monkeys received bilateral intravitreal (ivt) injection of dl-alpha-aminoadipic acid (dlaaa; 5 mg) following ophthalmic examination, color fundus photography, fluorescein angiography (fa) and optical coherence tomography (oct). ima ...202032275921
an intranasal exposure model of lethal nipah virus infection in african green monkeys.due to the difficulty in conducting clinical trials for vaccines and treatments against nipah virus (niv), licensure will likely require animal models, most importantly non-human primates (nhps). the nhp models of infection have primarily relied on intratracheal instillation or small particle aerosolization of niv. however, neither of these routes adequately models natural mucosal exposure to niv. to develop a more natural nhp model, we challenged african green monkeys with the bangladesh strain ...202031665362
a lethal aerosol exposure model of nipah virus strain bangladesh in african green monkeys.the high case-fatality rates and potential for use as a biological weapon make nipah virus (niv) a significant public health concern. previous studies assessing the pathogenic potential of niv delivered by the aerosol route in african green monkeys (agms) used the malaysia strain (nivm), which has caused lower instances of respiratory illness and person-to-person transmission during human outbreaks than the bangladesh strain (nivb). accordingly, we developed a small particle aerosol model of niv ...202031665351
leptospira infection in african green monkeys in an endemic area: an opportunity for comparative studies in a natural environment.this study was performed to investigate the potential asymptomatic leptospira reservoir status among african green monkeys (agms) in the caribbean island of saint kitts, and whether there is any renal pathology associated with leptospira exposure. forty-eight percent of agms tested were positive for leptospira antibodies by the microscopic agglutination test. leptospira dna was detected in 4% of kidney samples tested using a lipl32 gene based pcr. we observed minimal to severe microscopic renal ...202032560055
abundance, diversity, and distribution of primates at welel mountain, kellem wollega zone, oromia region, ethiopia.primates are the mammals of the order primate that is characterized by advanced development of binocular vision and enlargement of the cerebral hemispheres. the aim of this study was to investigate the abundance, diversity, and distribution of primates on welel mountain. from august 2017 to february 2018, we collected data from different parts of welel mountain during wet and dry seasons of the year and analyzed them using spss version 20. we identified four primate species: chlorocebus aethiops ...202032454802
diversity of medium and large mammals in the loka abaya national park, southern ethiopia.we evaluated the richness, diversity, and composition of the medium and large mammal community in the loka abaya national park (lanp), southern ethiopia, and how these parameters differ among four habitat types: wooded grassland, riverine forest, hilly scrubland and wetland, and between seasons. we recorded a total of 2,573 individual animals of 28 medium and large mammal species in the park. this included three globally threatened species: the endangered african wild dog (lycaon pictus), the vu ...202033005352
synthesis of pt(ii) complexes of the type [pt(1,10-phenanthroline)(sarfn)2] (sarfn = sc6h3-3,4-f2; sc6f4-4-h; sc6f5). preliminary evaluation of their in vitro anticancer activity.a series of pt(ii) complexes of the type [pt(1,10-phenanthroline)(sarfn)2] (sarfn = sc6h3-3,4-f2(1); sc6f4-4-h (2); sc6f5(3)) were synthesized from [pt(1,10-phenanthroline)(cl)2] and [pb(sarfn)2] via metathesis reactions. the complexes were fully characterized including the unambiguous determination of their molecular structures by single-crystal x-ray diffraction techniques, showing the metal centers to be into a slightly distorted square-planar environments. the in vitro cytotoxic activity of ...202032801098
reactive mesothelial hyperplasia mimicking mesothelioma in an african green monkey (chlorocebus aethiops).a spontaneous reactive mesothelial hyperplasia occurred in a female, 15.7-year-old african green monkey (grivet; chlorocebus aethiops). at necropsy, massive effusions were found in the abdomen, the thorax, and the pericardium. additionally, multiple small, beige-gray nodules were detected on the serosal surfaces of the abdominal organs. histopathologically, the mesothelial cells resembled the epithelioid subtype of a mesothelioma, but no infiltrative or invasive growth could be demonstrated. the ...202032760782
modeling mosquito-borne and sexual transmission of zika virus in an enzootic host, the african green monkey.mosquito-borne and sexual transmission of zika virus (zikv), a torch pathogen, recently initiated a series of large epidemics throughout the tropics. animal models are necessary to determine transmission risk and study pathogenesis, as well screen antivirals and vaccine candidates. in this study, we modeled mosquito and sexual transmission of zikv in the african green monkey (agm). following subcutaneous, intravaginal or intrarectal inoculation of agms with zikv, we determined the transmission p ...202032569276
resistance of cynomolgus monkeys to nipah and hendra virus disease is associated with cell-mediated and humoral immunity.the henipaviruses, hendra virus (hev) and nipah virus (niv), are capable of causing severe and often lethal respiratory and/or neurologic disease in animals and humans. given the sporadic nature of henipavirus outbreaks, licensure of vaccines and therapeutics for human use will likely require demonstration of efficacy in animal models that faithfully reproduce the human condition. currently, the african green monkey (agm) best mimics human henipavirus-induced disease.202032022850
mutational analysis of bfsp1, crybb1, galk1, and gja8 in captive-bred vervet monkeys (chlorocebus aethiops).congenital cataract has been reported in a colony of captive-bred vervet monkeys (chlorocebus aethiops).202031975409
persistent infection of simian foamy virus derived from the japanese macaque leads to the high-level expression of microrna that resembles the mir-1 microrna precursor family.micrornas (mirnas) are a group of small non-coding rnas that suppress the expression of target mrnas. the seed sequence of mirna plays a crucial role in recognizing the 3'-untranslated region of the target mrna. cells infected with a simian foamy virus (sfv) isolated from an african green monkey (chlorocebus aethiops) (sfvcae) showed high expression levels of viral mirnas encoded in the long terminal repeat of sfvcae. in the present study, we investigated the roles and expression of mirnas deriv ...202031969530
differences in frontal network anatomy across primate species.the frontal lobe is central to distinctive aspects of human cognition and behavior. some comparative studies link this to a larger frontal cortex and even larger frontal white matter in humans compared with other primates, yet others dispute these findings. the discrepancies between studies could be explained by limitations of the methods used to quantify volume differences across species, especially when applied to white matter connections. in this study, we used a novel tractography approach t ...202031949106
past and current advances in marburg virus disease: a review.marburg virus (marv), along with the ebola virus, belongs to the family of filovirus and is cause of a lethal and severely affecting hemorrhagic fever. the marburgvirus genus includes two viruses: marv and ravn. marv has been recognized as one of utmost importance by the world health organization (who). the case fatality rate of the virus ranges from 24.0 to 88.0% which demonstrates its lethal nature and the need for its widespread information. the first case of the marburgvirus disease (mard) w ...202032920568
rapid process development of serum-free pseudorabies virus production with the quality by design approach.this study described a successful application of the quality by design (qbd) approach to pseudorabies virus (prv) production process development in a fixed-bed bioreactor using the serum-free medium (sfm). the innovated tube-fixed-bed bioreactor was used as a scale-down model of the fixed-bed bioreactor for process development. risk analysis was performed using ishikawa diagram combined with failure mode effects analysis (fmea). the comparative experiment was performed to screen proper medium fo ...202032086694
influenza-infected newborn and adult monkeys exhibit a strong primary antibody response to hemagglutinin stem.the specificity of antibodies (abs) generated against influenza a virus (iav) infection can significantly alter protection and viral clearance. at present, the impact of age upon this process is relatively unexplored. here, we evaluated the ab response in newborn and adult african green monkeys following infection with iav using a strain that enables us to determine the immunodominance (id) hierarchy of the ab response to hemagglutinin (ha), the principal target of protective abs. this revealed ...202032078584
genome analysis and replication studies of the african green monkey simian foamy virus serotype 3 strain fv2014.african green monkey (agm) spumaretroviruses have been less well-studied than other simian foamy viruses (sfvs). we report the biological and genomic characterization of sfvcae_fv2014, which was the first foamy virus isolated from an african green monkey (agm) and was found to be serotype 3. infectivity studies in various cell lines from different species (mouse, dog, rhesus monkey, agm, and human) indicated that like other sfvs, sfvcae_fv2014 had broad species and cell tropism, and in vitro cel ...202032268512
nuphar lutea extracts exhibit anti-viral activity against the measles virus.different parts of nuphar lutea l. (yellow water lily) have been used to treat several inflammatory and pathogen-related diseases. it has shown that nuphar lutea extracts (nup) are active against various pathogens including bacteria, fungi, and leishmanial parasites. in an effort to detect novel therapeutic agents against negative-stranded rna (- rna) viruses, we have tested the effect of a partially-purified alkaloid mixture of nuphar lutea leaves on the measles virus (mv). the mv vaccine's edm ...202032260270
peanut applied to the skin of nonhuman primates induces antigen-specific igg but not ige.previous studies in humans support the dual-allergen exposure hypothesis, and several studies in mouse models have demonstrated that cutaneous exposure to disrupted or intact skin can lead to sensitization to peanut. however, the field lacks definitive evidence that cutaneous exposure leads to peanut allergy in humans or other primates.202032220059
a fine-scale genetic map for vervet monkeys.despite its important biological role, the evolution of recombination rates remains relatively poorly characterized. this owes, in part, to the lack of high-quality genomic resources to address this question across diverse species. humans and our closest evolutionary relatives, anthropoid apes, have remained a major focus of large-scale sequencing efforts, and thus recombination rate variation has been comparatively well studied in this group-with earlier work revealing a conservation at the bro ...202032211856
antimalarial 9-methoxystrobilurins, oudemansins, and related polyketides from cultures of basidiomycete favolaschia species.fourteen new compounds, oudemansins 1-4, oudemansinols 5-7, favolasins 8-10, favolasinin (12), polyketides 13-15, and (r,e)-2,4-dimethyl-5-phenyl-4-pentene-2,3-diol (16), together with nine known compounds were isolated from the basidiomycete fungus favolaschia sp. bcc 18686. two new compounds, favolasin e (11) and 9-oxostrobilurin e (17), were isolated from the closely related organism favolaschia calocera bcc 36684 along with nine β-methoxyacrylate-type derivatives. compounds in the class of o ...202032193929
cytotoxic effect of aminoglycoside antibiotics on the mammalian cell lines.aminoglycoside antibiotics have been used for treating serious but also routine infections in veterinary and human medicine for many years. the basic aim of this work is to evaluate the cytotoxicity of dihydrostreptomycin and neomycin in vitro on three cell cultures - bhk-21 (syrian golden hamster kidney fibroblast), vero (african green monkey kidney fibroblast) and fea (feline embryonic fibroblast) cells. the morphological changes were examined by giemsa staining. cells were dried and visualize ...202033040680
non-human primate determinants of natural killer cells in tissues at steady-state and during simian immunodeficiency virus infection.natural killer (nk) cells play essential roles in immunity to viruses and tumors. their function is genetically determined but also modulated by environmental factors. the distribution and functional regulation of these cells vary depending on the tissue. nk cell behavior in lymphoid tissues is so far understudied. non-human primate (nhp) models are essential for the development of therapies and vaccines against human diseases, and access to nhp tissues allows insights into spatial regulations o ...202033013901
potential for virus endogenization in humans through testicular germ cell infection: the case of hiv.viruses have colonized the germ line of our ancestors at several occasions during evolution, leading to the integration in the human genome of viral sequences from over 30 retroviral groups and a few non-retroviruses. among the recently emerged viruses infecting humans, several target the testis (eg hiv, zika and ebola viruses). here we aimed to investigate whether human testicular germ cells (tgcs) can support integration by hiv, a contemporary retrovirus that started to spread in the human pop ...202032999017
identification of schistosoma mansoni infection in a nonhuman primate from st. kitts more than 50 years after interruption of human transmission.transmission of schistosoma mansoni was interrupted on st. kitts, a caribbean island, in the 1950s. with no reported cases since that time and most biomphalaria spp. snail populations eliminated based on surveys in the 1970s, s. mansoni has been considered eliminated on st. kitts. in 2019, s. mansoni eggs were found in an african green monkey (chlorocebus aethiops sabaeus) that originated from st. kitts. nonhuman primate (nhp) infections have been considered incidental to human infections, with ...202032996451
aberrant expression of cell cycle regulator 14-3-3-σ and e-cadherin in a metastatic cholangiocarcinoma in a vervet monkey (chlorocebus pygerythrus).we present a unique case of metastatic cholangiocarcinoma with concurrent abdominal cestodiasis in an african green monkey (chlorocebus pygerythrus) that presented with respiratory insufficiency and abdominal discomfort. there were multiple white-grey masses in the liver and colonic serosa alongside intra-abdominal parasitic cysts. histopathologically, the liver masses were composed of poorly-differentiated epithelial cells that formed densely cellular solid areas and trabeculae. the neoplastic ...202032958143
sars-cov-2 infection of african green monkeys results in mild respiratory disease discernible by pet/ct imaging and shedding of infectious virus from both respiratory and gastrointestinal tracts.vaccines are urgently needed to combat the global coronavirus disease 2019 (covid-19) pandemic, and testing of candidate vaccines in an appropriate non-human primate (nhp) model is a critical step in the process. infection of african green monkeys (agm) with a low passage human isolate of sars-cov-2 by aerosol or mucosal exposure resulted in mild clinical infection with a transient decrease in lung tidal volume. imaging with human clinical-grade 18f-fluoro-2-deoxy-d-glucose positron emission tom ...202032946524
in-vitro study of hottentotta schach crude venom anticancer effects on mcf-7 and vero cell lines.scorpion venoms contain potentially useful pharmacological agents. several studies demonstrate that the venoms of some scorpions induce apoptosis and inhibit the growth of cancer cells; therefore, they have been investigated for isolating anticancer components. in this study, antitumor effects of hottentotta schach crude venom on mcf-7 (breast cancer cell line) as test group and vero (african green monkey kidney normal cell line) as control group were analyzed. cell toxicity was analyzed using m ...202032922480
genetic evidence for the role of non-human primates as reservoir hosts for human schistosomiasis.schistosomiasis is a chronic parasitic disease, that affects over 207 million people and causes over 200,000 deaths annually, mainly in sub-saharan africa. although many health measures have been carried out to limit parasite transmission, significant numbers of non-human primates such as chlorocebus aethiops (ch. aethiops) (vervet) and papio anubis (baboon) are infected with s. mansoni, notably in ethiopia, where they are expected to have potentially significant implications for transmission an ...202032898147
epigenetic silencing of cd4 expression in nonpathogenic siv infection in african green monkeys.african green monkeys (agms) are natural hosts of siv that postthymically downregulate cd4 to maintain a large population of cd4-cd8aa+ virus-resistant cells with th functionality, which can result in agms becoming apparently cured of sivagm infection. to understand the mechanisms of this process, we performed genome-wide transcriptional analysis on t cells induced to downregulate cd4 in vitro from agms and closely related patas monkeys and t cells that maintain cd4 expression from rhesus macaqu ...202032841214
sars-cov-2 infection dynamics in lungs of african green monkeys.detailed knowledge about the dynamics of sars-cov-2 infection is important for unraveling the viral and host factors that contribute to covid-19 pathogenesis. old-world nonhuman primates recapitulate mild-moderate covid-19 cases, thereby serving as important pathogenesis models. we compared african green monkeys inoculated with sars-cov-2 or inactivated virus to study the dynamics of virus replication throughout the respiratory tract. rna sequencing of single cells from the lungs and mediastinal ...202032839775
intranasal exposure of african green monkeys to sars-cov-2 results in acute phase pneumonia with shedding and lung injury still present in the early convalescence phase.we recently reported the development of the first african green monkey (agm) model for covid-19 based on a combined liquid intranasal (i.n.) and intratracheal (i.t.) exposure to severe acute respiratory syndrome coronavirus 2 (sars-cov-2). here, we followed up on this work by assessing an i.n. particle only route of exposure using the lma mucosal atomization device (mad). six agms were infected with sars-cov-2; three animals were euthanized near the peak stage of virus replication (day 5) and th ...202032818211
pseudorabies virus production using a serum-free medium in fixed-bed bioreactors with low cell inoculum density.fixed-bed bioreactors packed with macrocarriers show great potential to be used for vaccine process development and large-scale production due to distinguishing features of low shear force, high cell adhering surface area, and easy replacement of culture media in situ. as an initial step of utilizing this type of bioreactors for pseudorabies virus production (prv) by african green monkey kidney (vero) cells, we developed a tube-fixed-bed bioreactor in the previous study, which represents a scale ...202032816175
intranasal exposure of african green monkeys to sars-cov-2 results in acute phase pneumonia with shedding and lung injury still present in the early convalescence phase.we recently reported the development of the first african green monkey (agm) model for covid-19 based on a combined liquid intranasal (i.n.) and intratracheal (i.t.) exposure to severe acute respiratory syndrome coronavirus 2 (sars-cov-2). here, we followed up on this work by assessing an i.n. particle only route of exposure using the lma mucosal atomization device (mad). six agms were infected with sars-cov-2; three animals were euthanized near the peak stage of virus replication (day 5) and th ...202032811514
safety and immunogenicity of an intranasal sendai virus-based vaccine for human parainfluenza virus type i and respiratory syncytial virus (sevrsv) in adults.sevrsv is a replication-competent sendai virus (sev)-based vaccine carrying the respiratory syncytial virus (rsv) fusion protein (f) gene. unmanipulated, non-recombinant sev is a murine parainfluenza virus type 1 (piv-1) and serves as a jennerian vaccine for human piv-1 (hpiv-1). sev protects african green monkeys (agm) from infection after hpiv-1 challenge. the recombinant sevrsv additionally targets rsv and protects agm from lower respiratory infections after rsv challenge. the present study i ...202032750273
small particle aerosol exposure of african green monkeys to mers-cov as a model for highly pathogenic coronavirus infection.emerging coronaviruses are a global public health threat because of the potential for person-to-person transmission and high mortality rates. middle east respiratory syndrome coronavirus (mers-cov) emerged in 2012, causing lethal respiratory disease in »35% of cases. primate models of coronavirus disease are needed to support development of therapeutics, but few models exist that recapitulate severe disease. for initial development of a mers-cov primate model, 12 african green monkeys were expos ...202032744989
immune profiling of influenza-specific b- and t-cell responses in macaques using flow cytometry-based assays.influenza remains a significant global public health burden, despite substantial annual vaccination efforts against circulating virus strains. as a result, novel vaccine approaches are needed to generate long-lasting and universal broadly cross-reactive immunity against distinct influenza virus strains and subtypes. several new vaccine candidates are currently under development and/or in clinical trials. the successful development of new vaccines requires testing in animal models, other than mic ...202032741011
generation of pluripotent stem cells using somatic cell nuclear transfer and induced pluripotent somatic cells from african green monkeys.patient-specific stem cells derived from somatic cell nuclear transfer (scnt) embryos or from induced pluripotent stem cells (ipscs) could be used to treat various diseases with minimal immune rejection. many studies using these cells have been conducted in rats and mice; however, there exist numerous dissimilarities between the rodents and humans limiting the clinical predictive power and experimental utility of rodent experiments alone. nonhuman primates (nhps) share greater homology to human ...202032715987
biomarkers of senescence in non-human primate adipose depots relate to aging.forty-three female african green monkeys (chlorocebus aethiops sabaeus) were selected to represent young adult to advanced geriatric ages (7-24 years) to exhibit a wide range of obesity status (8-53% body fat) and diverse metabolic syndrome criteria such as diabetes, dyslipidemia, and hypertension. subcutaneous and visceral adipose tissues were collected and evaluated for the presence of senescence cells in both whole tissue and single-cell isolates from subcutaneous sources, utilizing senescenc ...202032705409
hydroxychloroquine use against sars-cov-2 infection in non-human primates.coronavirus disease 2019 (covid-19) has rapidly become a global pandemic and no antiviral drug or vaccine is yet available for the treatment of this disease1-3. several clinical studies are ongoing to evaluate the efficacy of repurposed drugs that have demonstrated antiviral efficacy in vitro. among these candidates, hydroxychloroquine (hcq) has been given to thousands of individuals infected with severe acute respiratory syndrome coronavirus 2 (sars-cov-2)-the virus that causes covid-19-worldwi ...202032698191
chloroquine does not inhibit infection of human lung cells with sars-cov-2.the coronavirus disease 2019 (covid-19) pandemic, which is caused by severe acute respiratory syndrome coronavirus 2 (sars-cov-2), has been associated with more than 780,000 deaths worldwide (as of 20 august 2020). to develop antiviral interventions quickly, drugs used for the treatment of unrelated diseases are currently being repurposed to treat covid-19. chloroquine is an anti-malaria drug that is used for the treatment of covid-19 as it inhibits the spread of sars-cov-2 in the african green ...202032698190
differentiation of trichuris species eggs from non-human primates by geometric morphometric analysis.human trichuriasis is a neglected tropical disease which affects millions of people worldwide, mostly living in low socio-economic conditions. numerous studies have been conducted over the past 10 years to compare the different techniques for t. trichiura eggs detection. our study provides the first geometric morphometric analysis for the specific detection of eggs of trichuris sp. isolated from stools of macaque (m. sylvanus), colobus (c. g. kikuyensis), grivets (c. aethiops) and the brazza's m ...202032695575
leptospira infections in domestic and wild animals.leptospirosis is a worldwide-distributed, re-emerging zoonosis due to the large variety of wild and domestic animal species that can play the role of natural or accidental host. currently, specific animal species play an important role as the reservoir for particular leptospira serovars, although recent investigations have highlighted new host-pathogen interactions involved in leptospira epidemiology. furthermore, the constant modification of ecosystems and wildlife habitats and the constantly i ...202032679834
data, reagents, assays and merits of proteomics for sars-cov-2 research and testing.as the covid-19 pandemic continues to spread, thousands of scientists around the globe have changed research direction to understand better how the virus works and to find out how it may be tackled. the number of manuscripts on preprint servers is soaring and peer-reviewed publications using ms-based proteomics are beginning to emerge. to facilitate proteomic research on sars-cov-2, the virus that causes covid-19, this report presents deep-scale proteomes (10,000 proteins; >130,000 peptides) of ...202032591346
advanced maternal age impacts physiologic adaptations to pregnancy in vervet monkeys.the trend to delay pregnancy in the usa has resulted in the number of advanced maternal age (ama) pregnancies to also increase. in humans, ama is associated with a variety of pregnancy-related pathologies such as preeclampsia (pe). while ama is known to be a factor which contributes to the development of pregnancy-induced diseases, the molecular and cellular mechanisms giving rise to this phenomenon are still very limited. this is due in part to lack of a preclinical model which has physiologic ...202032588342
the combined antibacterial and anticancer properties of nano ce-containing mg-phosphate ceramic.this paper was mainly aimed at synthesis of ce-containing nano-mg-phosphate ceramic as a multifunctional material.202032585244
live imaging of intra-lysosome ph in cell lines and primary neuronal culture using a novel genetically encoded biosensor.disorders of lysosomal physiology have increasingly been found to underlie the pathology of a rapidly growing cast of neurodevelopmental disorders and sporadic diseases of aging. one cardinal aspect of lysosomal (dys)function is lysosomal acidification in which defects trigger lysosomal stress signaling and defects in proteolytic capacity. we have developed a genetically encoded ratiometric probe to measure lysosomal ph coupled with a purification tag to efficiently purify lysosomes for both pro ...202032515674
establishment of an african green monkey model for covid-19.severe acute respiratory syndrome coronavirus 2 (sars-cov-2) is responsible for an unprecedented global pandemic of covid-19. animal models are urgently needed to study the pathogenesis of covid-19 and to screen candidate vaccines and treatments. nonhuman primates (nhp) are considered the gold standard model for many infectious pathogens as they usually best reflect the human condition. here, we show that african green monkeys support a high level of sars-cov-2 replication and develop pronounced ...202032511377
unusual mtdna control region length heteroplasmy in the cos-7 cell line.the cos-7 cell line is a workhorse of virology research. to expand this cell line's utility and to enable studies on mitochondrial dna (mtdna) transcription and replication, we determined the complete nucleotide sequence of its mitochondrial genome by sanger sequencing. in contrast to other available mtdna sequences from chlorocebus aethiops, the mtdna of the cos-7 cell line was found to contain a variable number of perfect copies of a 108 bp unit tandemly repeated in the control region. we esta ...202032486194
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