Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| effects of ph on transformation of bacillus subtilis with single-stranded deoxyribonucleic acid. | variation in frequencies of transformation mediated by native and single-stranded dna and its dependence on ph of the medium were investigated. the results indicate that the biological activity of deoxyribonucleic acid (dna) of both configurations assayed in the presence of ethylenediaminetetraacetic acid (edta) increases as the ph of the transformation mixture is lowered from 7.7 to a maximum transformation frequency near ph 6.1. at this lower ph, native dna transforms equally in medium with an ... | 1971 | 4997538 |
| relationship between competence for transformation of bacillus subtilis with native and single-stranded deoxyribonucleic acid. | the response of populations of bacillus subtilis to both native deoxyribonucleic acid (dna) and denatured dna was investigated at maximal competence and at various times during the development of compentency. the results indicate that competence for transformation with native and denatured dna increases and decreases simultaneously. competition occurs between native and single-stranded dna during transformation, and the same cells in a population can be doubly transformed by dna molecules of bot ... | 1971 | 4997539 |
| isolation and characterization of tricarboxylic acid cycle mutants of bacillus subtilis. | a technique was developed for the detection, on agar, of mutants of bacillus subtilis that lacked a functional tricarboxylic acid cycle. mutants devoid of detectable levels of aconitase, isocitric dehydrogenase, alpha-ketoglutarate dehydrogenase, succinic dehydrogenase, fumarase, and malate dehydrogenase have been isolated and characterized. several mutants with conditionally expressible lesions, including a mutant with a heat-sensitive citrate synthase, have also been isolated. all of the mutan ... | 1971 | 4997541 |
| repair of radiation damage to deoxyribonucleic acid in germinating spores of bacillus subtilis. | the repair of deoxyribonucleic acid (dna) in germinating spores was studied in comparison with that in vegetative cells. radiation-induced single-strand breaks in the dna of spores and of vegetative cells of bacillus subtilis were rejoined during postirradiation incubation. the molecular weight of single-stranded dna was restored to the level of nonirradiated cells. the rate of the rejoining of dna strand breaks in irradiated spores was essentially equal to that in irradiated vegetative cells. t ... | 1971 | 4997542 |
| variation in the primary structure of bacillus subtilis flagellins. | the flagella derived from 18 strains bacillus subtilis were tested for their reaction with antiflagellar filament antibody and antiflagellin antibody. on the basis of their reactivity, at least five serologically distinct classes could be identified. peptide map analysis of tryptic digests of the subunit proteins were consistent with the immunochemical analysis. large differences in sequence existed among proteins of the different classes; proteins within an antigenic group differed by only a fe ... | 1971 | 4997543 |
| minor threonine dehydratase encoded within the threonine synthetic region of bacillus subtilis. | challenging auxotrophs on metabolites that are precursors of a biosynthetic step involving a mutated enzyme has revealed a new class of suppressor mutations which act by derepressing a minor enzyme activity not normally detected in the wild-type strain. these indirect, partial suppressor mutations which allow isoleucine auxotrophs to grow on homoserine or threonine have been analyzed to determine their effect on enzymes involved in the biosynthesis of these amino acids. it has been found that on ... | 1971 | 4997544 |
| inhibition of bacterial dna replication by 6-(p-hydroxyphenylazo)-uracil: differential effect on repair and semi-conservative synthesis in bacillus subtilis. | 1971 | 4997657 | |
| the induction of a dehydrogenase activity for branched chain amino acids in bacillus subtilis. | 1971 | 4997675 | |
| [peptidyl-trna hydrolase: demonstration in various organisms. enzymatic activity in the presence of ribosomes]. | 1971 | 4997677 | |
| the binding of bacillus subtilis alkaline proteinases to alpha-2-macroglobulin. | 1971 | 4997785 | |
| [drug containers as possible carriers of infection in hospital]. | 1971 | 4997966 | |
| [synthesis of pyrolnitrin analogs. 3. preparation and antibacterial effects of substituted 1,2-diarylmaleimides]. | 1971 | 4997987 | |
| studies on mitomycins. v. synthesis of indolequinone and their activities. | 1971 | 4998036 | |
| [basic studies on determination method of ribostamycin (vistamycin meiji') in the blood and other body fluids]. | 1971 | 4998061 | |
| [case of tapeworm infection treated by paromomycin and observations on its mode of action]. | 1971 | 4998062 | |
| interactions of bacillus subtilis alkaline proteinases with alpha 2 -macroglobulin and alpha 1 -antitrypsin. | 1971 | 4998094 | |
| [repair of ultraviolet injury following irradiation of bacteria in a vacuum]. | 1971 | 4998751 | |
| in vitro labeling of polynucleotides by photoreduction. | 1971 | 4998786 | |
| purification and properties of an inhibitor of microbial alkaline proteinases from barley. | 1971 | 4998831 | |
| mapping the -aminolaevulinic acid synthetase locus in bacillus subtilis. | 1971 | 4999072 | |
| gene expression during outgrowth of bacillus subtilis spores. the relationship between gene order on the chromosome and temporal sequence of enzyme synthesis. | 1971 | 4999102 | |
| [mitomycin. 4. relationship between chemical structures and biological activities of mitomycin derivatives]. | 1971 | 4999105 | |
| rna synthesis during bacteriphage spo1 development. ii. some modulations and prerequisites of the transcription program. | 1971 | 4999125 | |
| control of dna synthesis in bacillus subtilis by phage phi e. | 1971 | 4999126 | |
| the chemistry of the cell walls of rod mutants of bacillus subtilis. | 1971 | 4999214 | |
| antigenic compositions of enzyme-containing preparations of bacillus subtilis origins. | 1971 | 4999249 | |
| fatty acid-requiring mutant of bacillus subtilis defective in branched chain alpha-keto acid dehydrogenase. | 1971 | 4999353 | |
| purification and properties of bacillus subtilis nucleoside diphosphokinase. | 1971 | 4999354 | |
| technology of a regional guthrie test service. | investigations were undertaken to discover the effect and importance of technical factors in the guthrie test and to determine the most satisfactory materials available. these findings were used in setting up the screening service for phenylketonuria for the south west metropolitan region. | 1971 | 4999357 |
| estimation of blood phenylalanine from a dried blood spot using the guthrie test. | 1971 | 4999358 | |
| isolation and characterization of a phosphatidylethanolamine-deficient mutnt of bacillus subtilis. | a mutant of bacillus subtilis atcc 6051 deficient in phosphatidylethanolamine, an important membrane lipid, was isolated by a combination of nitrosoguanidine mutagenesis and penicillin concentration of auxotrophs employing phosphatidylethanolamine as a supplement. the mutant was compared to the parent strain with regard to lipid composition, growth, osmotic fragility, and staining character and differed substantially in each category. in addition to scant amounts of phosphatidylethanolamine, the ... | 1971 | 4999411 |
| molecular sieving by the bacillus megaterium cell wall and protoplast. | passive permeabilities of the cell wall and protoplast of bacillus megaterium strain km were characterized by use of 50 hydrophilic probing molecules (tritiated water, sugars, dextrans, glycols, and polyglycols) which varied widely in size. weight per cent uptake values (r(w)) were measured at diffusional equilibrium under conditions that negated the influences of adsorption or active transport. plots of r(w) for intact cells as a function of number-average molecular weight ( m(n)) or einstein-s ... | 1971 | 4999413 |
| phospholipid metabolism in the absence of net phospholipid synthesis in a glycerol-requiring mutant of bacillus subtilis. | a glycerol-requiring auxotroph of bacillus subtilis showed no net synthesis of phospholipid when deprived of glycerol. although there was no net synthesis of phospholipid, we found that: (i) fatty acids and (32)p were slowly incorporated into phospholipid; (ii) in pulse-chase experiments, both (32)p and (14)c in the glycerol portion of the phospholipids were lost from phosphatidlyglycerol (pg) and lysylphosphatidylglycerol and accumulated in cardiolipin (cl); (iii) the proportions of the phospho ... | 1971 | 4999416 |
| protease activities during the course of sporulation on bacillus subtilis. | three proteolytic enzymes have been isolated from sporulating cultures of bacillus subtilis. these activities were, respectively, a protease inhibited by ethylenediaminetetraacetic acid (edta) but not phenylmethylsulfonyl fluoride (pmsf), a protease active on both protein and ester substrates, and an ester-active enzyme with low activity on proteins. the latter two enzymes were inhibited by pmsf but not by edta. the specific activity of each was determined both intra- and extra-cellularly during ... | 1971 | 4999417 |
| bacterial inactivation of transforming deoxyribonucleate. | competent and noncompetent bacteria are able to bind deoxyribonucleate (dna). the consequence of this binding is different for the two types of bacteria. competent bacteria are able to utilize the dna for transformation. dna exposed to noncompetent bacteria loses its biological activity with a coincident reduction in the double-stranded molecular weight. the reduction in molecular weight does not appear to be sufficient to account for the loss of biological activity observed. | 1971 | 4999418 |
| the role of bacterial antagonism and isoantagonism in the pattern of infection in chronic osteitis. | 1971 | 4999476 | |
| [effect of dnaases on apyrimidine dna]. | 1971 | 4999480 | |
| spore removal by centrifugation and its effect on ultra-high temperature commercial sterilization of milk. | 1971 | 4999495 | |
| ultrasonic synergistic effects in liquid-phase chemical sterilization. | new methods of sterilization employing a chemical with moderate heat and ultrasonic energy have been devised. inactivation of high-density bacterial spore suspensions is achieved by treatment with low concentration aqueous acid glutaraldehyde solutions at temperatures above or about 54 c. low (20 khz) or high (250 khz) frequency ultrasonic energy is synergistic with glutaraldehyde. rapid inactivation may also be achieved by using ultrasonic energy and aqueous alkalinized glutaraldehyde solutions ... | 1971 | 4999521 |
| streptomyces torulosus sp. n., an unusual knobby-spored taxon. | several strains of a streptomycete with unusual spore wall ornamentation were isolated from iowa soils. they are further characterized by coiled chains of spores, gray aerial mycelium, melanin-like pigments, and the production of antibacterial and antifungal antibiotics. spore wall ornamentations, as seen with the transmission electron microscope, do not correspond with any of the four generally recognized categories of spore wall ornamentation-smooth, warty, spiny, or hairy-but seem to represen ... | 1971 | 4999522 |
| effect of relative humidity and temperature on airborne venezuelan equine encephalitis virus. | inactivation of airborne venezuelan equine encephalitis (vee) virus disseminated from liquid suspensions or from lyophilized preparations as 1- to 5-mum particles was investigated under various conditions of relative humidity and temperature in a 2,500-liter static aerosol chamber. relative humidity ranging from 18 to 90% at 24 c and temperature ranging from -40 to 24 c had no marked effect on the biological decay rate or the recovery of viable airborne vee virus disseminated from liquid suspens ... | 1971 | 4999523 |
| surface decontamination in an autoclave chamber with formaldehyde gas. | a simple device for the conversion of a standard steam autoclave into a formalde-hyde gas sterilization chamber is described. | 1971 | 4999526 |
| bacillus subtilis septicemia: report of two cases. | 1971 | 4998512 | |
| [molecular-biological study on l-penicillamine]. | 1971 | 4998539 | |
| antitumor activity of 5-substituted 3-(1'-anilinoethylidene)pyrrolidine-2, 4-dione. | 1971 | 4998543 | |
| [thermophilic aerobic spore-forming hydrocarbon-utilizing oligonitrophilic bacteria and their physiology]. | 1971 | 4998584 | |
| utilization of deoxyribonucleoside triphosphates in the cellular synthesis of dna by bacillus subtilis. | 1971 | 4998622 | |
| a rapid method for removal of zinc from the metallo neutral proteases. | 1971 | 4998623 | |
| chromosom replication in toluenized bacillus subtilis cells. | 1971 | 4998703 | |
| studies on inosine-5-phosphate dehydrogenase of bacillus subtilis. purification and general properties. | 1971 | 4998714 | |
| purification and properties of dna-dependent rna polymerase from bacillus subtilis vegetative cells. | 1971 | 4999759 | |
| hybridization properties of dna sequences directing the synthesis of messenger rna and heterogeneous nuclear rna. | the relationship of the dna sequences from which polyribosomal messenger rna (mrna) and heterogeneous nuclear rna (nrna) of mouse l cells are transcribed was investigated by means of hybridization kinetics and thermal denaturation of the hybrids. hybridization was performed in formamide solutions at dna excess. under these conditions most of the hybridizing mrna and nrna react at values of d(o)t (dna concentration multiplied by time) expected for rna transcribed from the nonrepeated or rarely re ... | 1971 | 4999767 |
| [synthesis and biological activity of sulfur containing derivatives of chalcone. i. synthesis and antibacterial action of alkylthiochalcones]. | 1971 | 4999872 | |
| increased rate of asporogenous mutations following treatment of bacillus subtilis spores with ethyl methanesulfonate. | 1971 | 4999912 | |
| growth and cultivation of the unusual generalized transducing bacillus bacteriophage sp-15. | additional properties of sp-15, a generalized transducing bacteriophage notable for the ability to transfer an unusually large fragment of deoxyribonucleic acid (dna) to bacillus subtilis and b. licheniformis, are presented together with improved methods that enhance its utility. simple means have been found to provide the rigid control over moisture that is necessary for the assay of plaque-forming units (pfu). reproducible procedures for propagating transducing phage, which depend upon an appr ... | 1971 | 4999971 |
| [detection of inhibitors by bacteriological examination of meat]. | 1971 | 5000015 | |
| inactivation of genetic markers in transforming dna of bacillus subtilis by an enzyme which preferentially splits the guanine-guanine bonds in dna. | 1971 | 5000020 | |
| isolation and characterization of some humic acid decomposing bacteria and fungi from soil. | 1971 | 5000038 | |
| lysis of bacillus subtilis by bacteriophage sp82 in the absence of dna synthesis. | 1971 | 5002098 | |
| automated measurement of proteolytic enzymes. | 1971 | 5002134 | |
| dry-heat resistance of bacillus subtilis var. niger spores on mated surfaces. | bacillus subtilis var. niger spores were placed on the surfaces of test coupons manufactured from typical spacecraft materials (stainless steel, magnesium, titanium, and aluminum). these coupons were then juxtaposed at the inoculated surfaces and subjected to test pressures of 0, 1,000, 5,000, and 10,000 psi. tests were conducted in ambient, nitrogen, and helium atmospheres. while under the test pressure condition, the spores were exposed to 125 c for intervals of 5, 10, 20, 50, or 80 min, with ... | 1971 | 5002136 |
| thermal inactivation of aerosolized bacillus subtilis var. niger spores. | a hot-air sterilizer capable of exposing airborne microorganisms to elevated temperatures with an almost instantaneous heating time was developed and evaluated. with this apparatus, aerosolized bacillus subtilis var. niger spores were killed in about 0.02 sec when exposed to temperatures above 260 c. this is about 500 times faster than killing times reported by others. extrapolation and comparison of data on the time and temperature required to klll b. subtilis var. niger spores on surfaces show ... | 1971 | 5002138 |
| resistance of bacillus subtilis spores to inactivation by gamma irradiation and heating in the presence of a bactericide. 3. factors affecting rates of inactivation by phenylmercuric nitrate. | aqueous suspensions of bacillus subtilis nctc 8236 spores, surviving gamma irradiation from a cesium-137 source, exhibited an enhanced rate of inactivation compared to nonirradiated spores when heated with 0.04% phenylmercuric nitrate. the enhanced rate of inactivation, observable from survival curves, was noted when spores were irradiated with 150,000 rad under air in either the presence or absence of the bactericide. the magnitude of the enhanced inactivation rate increased as the irradiation ... | 1971 | 5002139 |
| disc plate method of microbiological antibiotic assay. i. factors influencing variability and error. | several factors are investigated that normally cause variation in zone diameters in conventional disc plate diffusion assay procedures. of these factors the most serious is the unequal exposure of the individual plates at top or bottom of stacks to temperatures above and below room temperature. this unequal temperature exposure is avoided by novel handling and incubation procedures. a major variable, but one which can be controlled, is the varying time interval between pouring seeded agar and th ... | 1971 | 5002143 |
| disc plate method of microbiological antibiotic assay. ii. novel procedure offering improved accuracy. | a detailed disc plate procedure is introduced for assay of antibiotics. the procedure is based on a previous study by the authors and deviates from conventional procedures in several respects: selected plastic petri dishes are employed; critical temperature control is simply provided at all stages of the test with refrigeration of the plates never used; all dilution is done with displacement microburettes; six pads (6.3 mm diameter) per dish are employed, all filled with the same unknown or refe ... | 1971 | 5002144 |
| [development of caulobacter in mixed cultures]. | 1971 | 5002168 | |
| [determination of the degree of adhesion of microbial cells to solid surfaces]. | 1971 | 5002169 | |
| [polynucleotide ligase activity of non-histone proteins of bone marrow cell chromatin]. | 1971 | 5001336 | |
| action of concanavalin a on the attachment stage of phagocytosis by macrophages. | 1971 | 5001381 | |
| a late replicating dna protein complex from cells in meiotic prophase. | 1971 | 5001382 | |
| adenosine triphosphate-dependent synthesis of biologically active dna by azide-poisoned bacteria. | nonviable cells of bacillus subtilis, when made permeable by treatment under controlled conditions with nonionic detergent, can be shown to replicate dna normally and to repair certain regions of the chromosome. the former process is stimulated by atp in the presence of datp, dctp, dgtp, and dttp. the product, a result of semiconservative replication, is biologically active; synthesis of the newly-formed regions of the chromosome appears to be sequential. | 1971 | 5001501 |
| phenylketonuria testing--its role in pediatrics and public health. | 1971 | 5001547 | |
| sporulation in bacillus subtilis. the appearance of sulpholactic acid as a marker event for sporulation. | 1. the synthesis of sulpholactic acid in sporulating cultures of bacillus subtilis was studied. 2. sulpholactic acid was first detected about 4h after the initiation of sporulation and 1h before refractility. the rate of synthesis paralleled that of the other events of sporulation examined. 3. sulpholactic acid accounted for 1.7% of the material of the spore. 4. because the addition of chloramphenicol in the earlier stages of sporulation inhibited formation of the compound, it is likely that the ... | 1971 | 5001733 |
| the interaction of concanavalin a with teichoic acids and bacterial walls. | 1971 | 5001734 | |
| sporulation in bacillus subtilis 168. comparison of alkaline phosphatase from sporulating and vegetative cells. | 1. the purification of the ;vegetative' alkaline phosphatase of bacillus subtilis 168 was simplified by ionic elution of the enzyme from intact cells. 2. the enzyme has a molecular weight of about 70000 and treatment of the enzyme with 10mm-hydrochloric acid or 6.0m-guanidine hydrochloride, beta-mercaptoethanol (0.1m) gives rise to enzymically inactive subunits. 3. the amino acid composition of the enzyme was determined. the n-terminal residue determined by the dns chloride method is glycine. 4. ... | 1971 | 5001777 |
| [glycosides with lactone-forming aglycones. v. nartheside a and b, 2 diastereomeric lactone glucosides and their antibiotic active aglycone from bog asphodel (narthecium ossifragum hud.)]. | 1971 | 5001781 | |
| [analysis of internucleotide breakages, repairable by polynucleotide ligase, in dna irradiated by gamma-rays]. | 1971 | 5001782 | |
| the chromosome of bacillus subtilis. ii. a general solution for the age distribution function of bacterial chromosomes. | 1971 | 5001784 | |
| nifurpipone, a new nitrofuran with a large antimicrobial spectrum. | 1971 | 5001825 | |
| the enzymic formation of o-acetylhomoserine in bacillus subtilis and its regulation by methionine and s-adenosylmethionine. | 1971 | 5001847 | |
| [kinetics of serine proteases from microorganisms]. | 1971 | 5001865 | |
| macromolecular synthesis in bacillus subtilis during development of the competent state. | rates of deoxyribonucleic acid, ribonucleic acid, and protein synthesis were examined in purified competent cells of bacillus subtilis during the development of the transformable state. to become competent, a cell must depart from the normal course of vegetative growth and pass through a precompetent phase beginning as early as 90 to 180 min before the appearance of transformability. while in the precompetent state, the cell decreases its rate of deoxyribonucleic acid synthesis and lowers its ra ... | 1971 | 5001867 |
| fate of transforming deoxyribonucleate in bacillus subtilis. | the majority of donor deoxyribonucleate (dna) at early stages after uptake was found in a complex with a cell component which changes its buoyant behavior on equilibrium density gradients. analysis of the recipient cell lysates, after treatment to dissociate the complex, showed about two-thirds of the donor molecules in denatured form and the rest associated with recipient dna. incubation of cells after dna uptake leads to the disappearance of denatured donor dna and to the increase of donor lab ... | 1971 | 5001868 |
| [splitting of pyr-3'-p-5'-pur bond in dna by dnaases of some bacteria]. | 1971 | 5001936 | |
| [relation of aerobic spore-forming thermophilic bacteria to low-boiling n-alkanes (c6-c10)]. | 1971 | 5001937 | |
| mutagenic action of edeine in bacteria. ii. induction of streptomycin resistance in escherichia coli and bacillus subtilis. | 1971 | 5001945 | |
| enzymic explorations of the structures of starch and glycogen. | 1971 | 5001952 | |
| heat induction of prophage phi 105 in bacillus subtilis: replication of the bacterial and bacteriophage genomes. | a temperature-inducible mutant of temperate bacillus bacteriophage phi105 was isolated and used to lysogenize a thymine-requiring strain of bacillus subtilis 168. synthesis of phage and bacterial deoxyribonucleic acid (dna) was studied by sucrose gradient centrifugation and density equilibrium centrifugation of dna extracted from induced bacteria. the distribution of dna in the gradients was measured by differential isotope and density labeling of dna before and after induction and by measuring ... | 1971 | 5002012 |
| [study of the growth of the bacillus subtilis membrane by means of flagella distribution]. | 1971 | 5002044 | |
| [localization of levansucrase in bacillus subtilis var. niger]. | 1971 | 5002045 | |
| sterility indicator with artificial resistance to ethylene oxide. | 1971 | 4998189 | |
| identification of two lysine trna cistrons in bacillus subtilis by hybridization of lysyl-trna with dna. | 1971 | 4998233 | |
| effect of sulfhydryl reagents on the ribosomes of bacillus subtilis. | the effect of various sulfhydryl reagents on the ribosomes of bacillus subtilis was studied. the 70s ribosomes were completely dissociated into 30s and 50s subunits by appropriate concentrations of p-chloromercuribenzoic acid (pcmb) and 5,5'-dithio-bis-(2-nitro-benzoic acid). the n-ethylmaleimide and iodoacetamide failed to dissociate the ribosomes even at relatively high concentrations. the rate of dissociation of ribosomes by pcmb varied with the concentration of ribosomes. a progressive decre ... | 1971 | 4998249 |
| synthesis of neomycin by washed mycelium of streptomyces fradiae and some physiological considerations. | 1971 | 5000099 | |
| rabbit platelet bactericidal protein. | the heat-stable antibacterial activity of rabbit serum against gram-positive microorganisms has been shown to reside in a cationic protein fraction of platelet lysosomal granules. the activity is released during platelet aggregation. no plasma or serum component is required for the bactericidal effect. the platelet bactericidin resembles the antibacterial proteins of leukocyte granules both in cellular localization and in biochemical characteristics. it can be differentiated from platelet factor ... | 1971 | 5000106 |
| abortive infection of sporulating bacillus subtilis 168 by phi 2 bacteriophage. | bacteriophage phi2 is unable to replicate in bacillus subtilis 168. although some phage deoxyribonucleic acid (dna) synthesis can occur, the dna made is not biologically active and sedimentation analysis reveals that it is smaller in size than that of mature dna or dna isolated from phi2-infected permissive hosts. messenger ribonucleic acid hybridizable with phi2 dna is also synthesized in phi2-infected cells of 168. mutants of 168 which are permissive hosts for phi2 have been isolated. these mu ... | 1971 | 5000116 |
| the sigma factors of rna polymerase. | 1971 | 5000226 | |
| [electron microscope study of cell wall growth in b. subtilis and b. megaterium]. | 1971 | 5000248 | |
| primary sequence of trna-val-1 from escherichia coli b. ii. isolation of large fragments by limited digestion with rnases, and overlapping of fragments to reduce the total primary sequence. | 1971 | 5000876 | |
| alterations in the structure of deoxyribonucleic acid on chemical methylation. | 1971 | 5000877 |