Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| characterization of pcr products from bacilli using electrospray ionization fticr mass spectrometry. | a procedure for rapid purification of polymerase chain reaction (pcr) products allowing precise molecular weight determination using electrospray ionization-fourier transform ion cyclotron resonance (esi-fticr) mass spectrometry is described. pcr amplification utilized the dna polymerase from pyrococcus furiosus (pfu) which, unlike taq, does not incorporate a nontemplated terminal deoxyadenosine phosphate. an 89-base pair nucleotide portion of the spacer region between the 16s and 23s ribosomal ... | 1996 | 8914480 |
| comparison of the beta-toxins from staphylococcus aureus and staphylococcus intermedius. | the beta-toxins produced by staphylococcus aureus and staphylococcus intermedius were purified to homogeneity from culture supernatants. although the toxin from s. aureus has been throughly studied, less is known about its unique counterpart from s. intermedius. this is the first reported purification and analysis of the s. intermedius beta-toxin. both toxins have similar enzymatic properties, belong to the class of neutral sphingomyelinases c, and have a high specificity for sphingomyelin. they ... | 1996 | 8914839 |
| mouse lethal activity of a hep-2 vacuolation factor, cereulide, produced by bacillus cereus isolated from vomiting-type food poisoning. | the hep-2 vacuolation factor (or cereulide) produced by bacillus cereus isolated from vomiting-type food poisoning, which is supposed to induce emesis, was found to give mouse and suncus lethality after intravenous and intraperitoneal administration. the emetic activity of the factor was also found to be resistant to heating at 121 degrees c for 15 min, exposure to ph 2 and 11, and to digestion with proteolytic enzymes such as pepsin and trypsin. these findings suggest that the cereulide produce ... | 1996 | 8916007 |
| killing of microorganisms by pulsed electric fields. | lethal effects of pulsed electric fields (pef) on suspensions of various bacteria, yeast, and spores in buffer solutions and liquid foodstuffs were examined. living-cell counts of vegetative cell types were reduced by pef treatment by up to more than four orders of magnitude (> 99.99%). on the other hand, endo- and ascospores were not inactivated or killed to any great extent. the killing of vegetative cell types depends on the electrical field strength of the pulses and on the treatment time (t ... | 1996 | 8920190 |
| evaluation of swirling, ph, and glucose tests for the detection of bacterial contamination in platelet concentrates. | although infrequent, episodes of transfusion-associated bacterial sepsis may lead to serious outcomes or death and therefore are of concern. this study evaluates the sensitivity of three surrogate tests for the presence of bacteria in platelet concentrates: cessation of swirling, low extracellular ph, and low plasma glucose levels. | 1996 | 8937410 |
| is the exploding powder gas of the propellant from blank cartridges sterile? | shots from blank weapons loaded with blank cartridges, when fired from close range or as a contact shot, almost always cause the skin to burst open and lead to injuries to structures below the surface. subsequently, wound infections are often observed. in addition to the introduction of skin germs, the possibility exists that contaminated propellants may enter into consideration as a source of infection. using step-by-step experimental procedures we were able to demonstrate that: 1. blank cartri ... | 1996 | 8939008 |
| an alkaline d-stereospecific endopeptidase with beta-lactamase activity from bacillus cereus. | we purified a novel extracellular d-stereospecific endopeptidase, alkaline d-peptidase (d-stereospecific peptide hydrolase, ec 3.4.11.-), to homogeneity from the culture broth of the soil bacterium bacillus cereus strain df4-b. the mr of the enzyme was 37,952, and it was composed of a single polypeptide chain. the optimal ph for activity was approximately 10.3. the enzyme was strictly d-stereospecific toward oligopeptides composed of dphenylalanine such as (d-phe)3 and (d-phe)4. the enzyme also ... | 1996 | 8939979 |
| vanadate is a potent competitive inhibitor of phospholipase c from bacillus cereus. | monomeric vanadate is a potent competitive inhibitor of phospholipase c from bacillus cereus, much better than other oxyanions (e.g., phosphate or iodate). the apparent efficiency of inhibition depends on the substrate aggregate structure. the measured inhibition constant with respect to monomeric phosphatidylcholine substrate is 0.21 mm under conditions where the k(m) is 0.12 mm; for micellar substrate the apparent ki appears much lower and in fact tracks the apparent k(m) which decreases 10-fo ... | 1996 | 8948489 |
| experimental bacillus cereus post-traumatic endophthalmitis and treatment with ciprofloxacin. | bacillus species remain an important cause of post-traumatic endophthalmitis, often causing permanent visual loss. | 1996 | 8949723 |
| origin of the lag period in the phospholipase c cleavage of phospholipids in membranes. concomitant vesicle aggregation and enzyme activation. | when phospholipase c is added to a suspension of large unilamellar vesicles of egg phosphatidylcholine, maximal rates of hydrolysis occur only after a latency period. no lag period is seen when the substrate is in the form of small (sonicated) vesicles, or of short-chain phosphatidylcholine monomers. for a given vesicle concentration, the lag time may vary as a function of ca2+, enzyme concentration, or temperature, but activation occurs at a fixed molar fraction of diacylglycerol produced. lag ... | 1996 | 8952465 |
| mobilization of "nonmobilizable" plasmids by the aggregation-mediated conjugation system of bacillus thuringiensis. | the aggregation-mediated conjugation system of bacillus thuringiensis subsp, israelensis (bti), encoded by the 200-kb plasmid pxo16, is highly potent in transferring itself and efficient in mobilizing other nonconjugative plasmids. in the present study we have analyzed the native bacillus cereus plasmid pbc16. this plasmid has previously been shown to harbor a mob gene (orf beta) and a locus functioning as an orit site in plasmid pls20-mediated conjugation in bacillus subtilis. however, in the c ... | 1996 | 8954879 |
| polyclonal catalytic anti-idiotypic antibodies with a beta-lactamase activity. | 1996 | 8958088 | |
| mechanism of action of hemolysin iii from bacillus cereus. | bacillus cereus hemolysin iii activity was tested in crude extracts, from escherichia coli carrying the hly-iii gene. it was concluded that hemolysin iii is a pore-forming hemolysin with functional pore diameter of about 3-3.5 nm. hemolysis occurs in at least three steps: (i) the temperature-dependent binding of the hly-iii monomers to the erythrocyte membrane; (ii) the temperature-dependent formation of the transmembrane oligomeric pore; (iii) the temperature-independent erythrocyte lysis. | 1996 | 8962879 |
| successful visual recovery in delayed onset bacillus cereus endophthalmitis. | 1996 | 8630757 | |
| identification and purification of a family of dimeric major cold shock protein homologs from the psychrotrophic bacillus cereus wsbc 10201. | whole-cell protein patterns of a psychrotrophic bacillus cereus strain from cultures grown at 7 and 30 degrees c were compared. this analysis revealed that at least three major proteins are expressed at a significantly higher rate at 7 degrees c than at 30 degrees c. the most abundant of these cold-induced proteins was a small polypeptide of 7.5 kda, designated cspa, of b. cereus. in addition, four small proteins very similar in size to cspa were seen on both 7 degrees c and 30 degrees c two-dim ... | 1996 | 8631682 |
| tincture of benzoin: clinical and microbiological implications of reusable containers. | at our institution, tincture of benzoin solution is commonly used as a topical adhesive agent. as a cost-saving practice, multiple-dose bottles are routinely used in the operating rooms and the clinic on multiple patients. although clinically pathogenic organisms are known to be capable of survival in both benzoin and its isopropyl alcohol solvent, no prior controlled studies have investigated the potential for tincture of benzoin solution to support the growth of specific pathogens under clinic ... | 1996 | 8637641 |
| beta-secondary and solvent deuterium kinetic isotope effects on beta-lactamase catalysis. | beta-secondary and solvent deuterium kinetic isotope effects have been determined for the steady-state kinetic parameters v/k and v for turnover of a depsipeptide substrate, m-[[(phenylacetyl)glycyl]-oxy]benzoic acid, and of a beta-lactam substrate, penicillanic acid, by three typical class a beta-lactamases and a class c beta-lactamase. the isotope effects on alkaline hydrolysis of these substrates have been used as a frame of reference. the effect of the transition state conformation of the su ... | 1996 | 8639512 |
| syntheses and antimicrobial activities of five-membered ring heterocycles coupled to indole moieties. | indole-substituted oxazolidinones, oxazolones, pyrrolidinone, imidazolidinone and imidazolones were synthesized. their inhibitory potencies towards protein kinase c and protein kinase a were determined and their in vitro activities against streptomyces chartreusis, streptomyces griseus, bacillus cereus, candida albicans and escherichia coli were examined. the inhibition of streptomyces sporulation observed for some of them could not be linked to in vitro protein kinase c inhibition. all proved i ... | 1996 | 8642002 |
| dual inhibitory effect of gangliosides on phospholipase c-promoted fusion of lipidic vesicles. | the effect of a variety of gangliosides has been tested on the phospholipase c-induced fusion of large unilamellar vesicles. bilayer composition was phosphatidylcholine:phosphatidylethanolamine: cholesterol (2:1:1 mole ratio) plus the appropriate amounts of glycosphingolipids. enzyme phosphohydrolase activity, vesicle aggregation, mixing of bilayer lipids and mixing of liposomal aqueous contents were separately assayed. small amounts ( < 1 mol %) of gangliosides in the lipid bilayer produce a si ... | 1996 | 8652529 |
| sepsis associated with blood transfusion. | 1996 | 8656939 | |
| production of an emetic toxin, cereulide, is associated with a specific class of bacillus cereus. | the emetic toxin (cereulide) of bacillus cereus was quantified in several isolates of b. cereus and in various food sources. when the emetic toxin was produced, vomiting-type food poisoning was observed in humans. we also found that the h-1 serovar phenotype was strongly associated with the production of cereulide and that none of the isolates that hydrolyzed starch or expressed diarrheal enterotoxin activity produced cereulide. | 1996 | 8661692 |
| phosphatidylinositol phospholipase c is activated allosterically by the aminoglycoside g418. 2-deoxy-2-fluoro-scyllo-inositol-1-o-dodecylphosphonate and its analogs inhibit glycosylphosphatidylinositol phospholipase c. | phosphatidylinositol-specific phospholipase c (pi-plc) from bacillus cereus is inhibited by myo-inositol-1-o-dodecylphosphonate (ins-1-o-dodecylphosphonate) (morris, j. c., ping-sheng, l., shen, t. y., and mensa-wilmot, k.(1995) j. biol. chem. 270, 2517-2524). a set of novel fluorinated 2-deoxy-ins-1-o-dodecylphosphonates were tested against pi-plc, with potent competitive inhibition by 2-deoxy-2-fluoro-scyllo-ins-1-o-dodecylphosphonate (vp-616l) (xi(50) = 0.09). 2-deoxy-2-fluoro-myo-ins-1-o-dod ... | 1996 | 8663028 |
| useful visual outcomes after treatment of bacillus cereus endophthalmitis. | bacillus cereus endophthalmitis occurring after penetrating ocular trauma has been almost always associated with a poor visual outcome. the purpose of our study was to review and report patients who had useful visual acuity outcomes. | 1996 | 8600414 |
| analysis of double-stranded polymerase chain reaction products from the bacillus cereus group by electrospray ionization fourier transform ion cyclotron resonance mass spectrometry. | the analysis of polymerase chain reaction (pcr) products by electrospray ionization-fourier transform ion cyclotron resonance mass spectrometry (esi-fticr) has been achieved. specifically, a 105 base-pair nucleotide portion of the ribosomal spacer region was amplified in two members of the b. cereus group (i.e. b. thuringiensis and b. cereus) using pcr. these amplified regions were then analyzed by gel electrophoresis and esi-fticr. based on the predicted sequence of the pcr products for each or ... | 1996 | 8563015 |
| ionophore properties of monensin derivatives studied on human erythrocytes by 23na nmr and k+ and h+ potentiometry: relationship with antimicrobial and antimalarial activities. | eight derivatives of monensin with a modified c25-c26 moiety were synthesized. their ionophore properties were studied on human erythrocytes by measuring na+ influx with 23na nmr and concomitant k+ and h+ efflux by potentiometry. modification of oh-26 led to inversion of selectivity of transport in favor of k+/na+ in comparison with monensin. this selectivity disappeared by suppression of the c26-oh moiety. finally the ionophore ability was lost if the head-to-tail chelation of the monensin skel ... | 1996 | 8558531 |
| continuous enzymatic production of xylitol with simultaneous coenzyme regeneration in a charged membrane reactor. | we have developed a new process for the production of xylitol from d-xylose by enzyme technology. an nadh-dependent xylose reductase (xr) from candida tenuis catalyzes the reduction of xylose, which is coupled to enzymatic oxidations of d-glucose or d-xylose by glucose dehydrogenase from bacillus cereus to make achievable an up to 10,000-fold regeneration of nadh per cycle of discontinuous conversion. using a simple kinetic model as a tool for process optimization, suitable conditions with regar ... | 1996 | 18629908 |
| bacillus thuringiensis hd-73 spores have surface-localized cry1ac toxin: physiological and pathogenic consequences. | spores from cry(sup+) strains of bacillus thuringiensis bound fluorescein isothiocyanate-labeled antibodies specific for the 65-kda activated cry 1ac toxin, whereas spores from bacillus cereus and cry(sup-) strains of b. thuringiensis did not. the cry(sup+) spores could be activated for germination by alkaline conditions (ph 10.3), whereas cry(sup-) spores could not. once the surrounding exosporia had been removed or permeabilized, cry(sup+) spores were able to bind the toxin receptor(s) from in ... | 1996 | 16535421 |
| e144s active-site mutant of the bacillus cereus thermolysin-like neutral protease at 2.8 a resolution. | the x-ray crystal structure of the bacillus cereus neutral protease (cnp) active-site mutant e144s, in which the putative general base proposed for the thermolysin-like zinc neutral proteases, glu144, has been replaced by serine, has been determined to a resolution of 2.8 a. this represents the first crystal structure of an active-site mutant of a zinc neutral protease. the e 144s mutant was crystallized in the hexagonal space group, p6(5)22, with unit-cell dimensions a = b = 76.57, c = 201.91 a ... | 1996 | 15299677 |
| the antimicrobial properties of chile peppers (capsicum species) and their uses in mayan medicine. | a survey of the mayan pharmacopoeia revealed that tissues of capsicum species (solanaceae) are included in a number of herbal remedies for a variety of ailments of probable microbial origin. using a filter disk assay, plain and heated aqueous extracts from fresh capsicum annuum, capsicum baccatum, capsicum chinese, capsicum frutescens, and capsicum pubescens varieties were tested for their antimicrobial effects with fifteen bacterial species and one yeast species. two pungent compounds found in ... | 1996 | 8735449 |
| [bacteremia associated with implantable device for central venous access caused by bacillus cereus]. | 1996 | 8744378 | |
| a distant evolutionary relationship between bacterial sphingomyelinase and mammalian dnase i. | the three-dimensional structure of bacterial sphingomyelinase (smase) was predicted using a protein fold recognition method; the search of a library of known structures showed that the smase sequence is highly compatible with the mammalian dnase i structure, which suggested that smase adopts a structure similar to that of dnase i. the amino acid sequence alignment based on the prediction revealed that, despite the lack of overall sequence similarity (less than 10% identity), those residues of dn ... | 1996 | 8976554 |
| use of cluster and discriminant analyses to compare rhizosphere bacterial communities following biological perturbation | we present an approach to comparing the diversity and composition of bacterial communities from different habitats and for identifying which members of a community are most affected by an introduced bacterium. we use this method to explore both previously published and new data from field and growth chamber experiments in which we isolated heterotrophic bacteria from samples of root-free soil, roots of nontreated soybean seedlings, and from the roots of soybean seedlings grown from bacillus cere ... | 1996 | 8688005 |
| food-borne disease outbreaks in taiwan, 1994. | in 1994, 102 outbreaks of food-borne disease involving 4,726 cases were reported to the taiwan department of health. this is the highest number of outbreaks and cases in recent years in taiwan. of these outbreaks, 72.5% (74/102) were caused by bacterial pathogens, with vibrio parahaemolyticus responsible for 56.7% (42/74), staphylococcus aureus 20.3% (15/74), bacillus cereus 14.9% (11/74) and salmonella spp other than s. typhi and s. paratyphi 8.1% (6/74). v. parahaemolyticus has been a leading ... | 1996 | 8688712 |
| cloning, sequencing, and expression of a beta-amylase gene from bacillus cereus var. mycoides and characterization of its products. | the cloned gene was composed of 1638 bp for coding plus promoter like and sd-like sequences ahead of it. the deduced amino acid sequence had high similarity with known beta-amylases. the n-terminal sequence of the cloned beta-amylase seemed to be a signal peptide. the gene was introduced into bacillus subtilis 1a289 using phy300plk as a vector and the expressed protein was recovered from the culture media. the enzyme fraction produced was divided into two components upon the deae column chromato ... | 1996 | 8987540 |
| accumulation of benzoic acid in suspension cultured cells of pinus thunbergii parl. in response to phenylacetic acid administration. | the generation and accumulation of both benzoic acid (ba) and its conjugates were induced in suspension cultured cells of pinus thunbergii by administering either phenylacetic acid (pa), a toxic metabolite of bacillus cereus (strain hy-3) accompanying the pine wood nematode, or a lyophilized culture supernatant of this bacterium. ba conjugates reached their maximal levels in quantity two days after the administration and then decreased gradually until the 14th day, while ba increased significant ... | 1996 | 8987587 |
| two investigational glycylcyclines, dmg-dmdot and dmg-mino. antimicrobial activity studies against gram-positive species. | dmg-dmdot (cl-331,002 or cl-331,928) and dmg-mino (cl-329,998 or cl-344,677) are two new semisynthetic tetracyclines called glycylcyclines, with a broad spectrum of activity and includes enterobacteriaceae, gram-positive cocci, jk diphtheroids, and bacillus cereus. potent activity was demonstrated against all streptococcus spp. strains [minimum inhibitory concentrations] (mic90s) 0.06-0.25 micrograms/ml) and staphylococci (oxacillin susceptible ans resistant; mic90s 0.12-2 micrograms/ml). both g ... | 1996 | 8988765 |
| risk factors for outbreaks of infectious intestinal disease linked to domestic catering. | the epidemiology of general outbreaks of infectious intestinal disease associated with domestic catering for large numbers is described and compared with foodborne outbreaks in other settings. from 1 january 1992 to 31 december 1994, the phls communicable disease surveillance centre identified 101 foodborne general outbreaks of infectious intestinal disease associated with domestic catering in england and wales (16% of all foodborne outbreaks). salmonella species were associated with 77 of the 1 ... | 1996 | 8990573 |
| [site-specific bcuai endonuclease from bacillus cereus a]. | a new restriction endonuclease was isolated from the bacillus cereus bkm b-814 by means of the cell disruption with ultrasonication, ammonium sulfate fractionation of the cell-free extract, and chromatography on deae-sepharose to give about 1400 u of the enzyme per gram of cells. the enzyme revealed the maximum activity at 30-37 degrees c, ph 7.6-8.2, and 5-10 mm mgcl2 under a high ionic strength (50 mm tris-hcl, 100 mm nacl). the site-specific endonuclease bcuai was found to recognize the 5' g ... | 1996 | 8992958 |
| [exotoxins of bacillus cereus associated with food poisoning]. | 1996 | 8994344 | |
| incidence of diarrhea with antibiotics and the increase of clostridia in rabbits. | rabbits were treated with a single intravenous injection of various antibiotics. more than 40 per cent of the animals showed diarrhea after being treated with sulbactam/cefoperazone, cefmetazole, clindamycin, piperacillin or aspoxicillin. clostridium difficile was isolated from sulbactam/cefoperazone-treated diarrheic rabbits, with their cecal contents showing positive reaction in a latex agglutination test for c. difficile enterotoxin. however, 27 cefmetazole-induced diarrheic cases were not as ... | 1996 | 8996700 |
| a germination-specific spore cortex-lytic enzyme from bacillus cereus spores: cloning and sequencing of the gene and molecular characterization of the enzyme. | a gene (sleb) encoding a 24-kda germination-specific spore cortex-lytic enzyme, probably an n-acetylmuramyl-l-alanine amidase, was cloned from bacillus cereus, and its nucleotide sequence was determined. it was indicated that the enzyme is produced as a 259-residue protein with a signal sequence of 32 residues and is present in dormant spores in its active form. sulfhydryl reagents inactivated the enzyme, but mutation of a single cysteine of the protein, cys-258, to gly did not cause complete in ... | 1996 | 8752358 |
| crystal structure of phosphatidylinositol-specific phospholipase c from bacillus cereus in complex with glucosaminyl(alpha 1-->6)-d-myo-inositol, an essential fragment of gpi anchors. | numerous proteins on the external surface of the plasma membrane are anchored by glycosylated derivatives of phosphatidylinositol (gpi), rather than by hydrophobic amino acids embedded in the phospholipid bilayer. these gpi anchors are cleaved by phosphatidylinositol-specific phospholipases c (pi-plcs) to release a water-soluble protein with an exposed glycosylinositol moiety and diacylglycerol, which remains in the membrane. we have previously determined the crystal structure of bacillus cereus ... | 1996 | 8755729 |
| selective killing of human monocytes and cytokine release provoked by sphingomyelinase (beta-toxin) of staphylococcus aureus. | the best-known activity of staphylococcus aureus sphingomyelinase c, alias beta-toxin, is as a hemolysin that provokes hot-cold lysis of erythrocytes which contain substantial amounts of sphingomyelin in the plasma membrane. sheep erythrocytes are most susceptible, and we found that one hemolytic unit, representing the toxin concentration that elicits 50% hemolysis of 2.5 x 10(8) erythrocytes per ml, corresponds to 0.05 enzyme units or to approximately 0.25 microg of sphingomyelinase per ml. the ... | 1996 | 8757823 |
| [construction of shuttle vector containing delta-endotoxin gene of bacillus thuringiensis]. | the bacillus thuringiensis toxin gene cryia(c) was inserted into the shuttle vector pbe-2 to construct pamy for expressing the b.t. gene in both gram-negative and -positive bacterial systems. pamy was introduced into wild type bacillus cereus, b.brevis and b.subtilis by electroporation. transformants containing delta-endotoxin gene produced proteins reacted with b.t. crystal protein antibody. upon biological toxicity tests, the transformants gave a mortality of 100% against ostrinia furnacilis, ... | 1996 | 8762474 |
| zwittermicin a resistance gene from bacillus cereus. | zwittermicin a is a novel aminopolyol antibiotic produced by bacillus cereus that is active against diverse bacteria and lower eukaryotes (l.a. silo-suh, b.j. lethbridge, s.j. raffel, h. he, j. clardy, and j. handelsman, appl. environ. microbiol. 60:2023-2030, 1994). to identify a determinant for resistance to zwittermicin a, we constructed a genomic library from b. cereus uw85, which produces zwittermicin a, and screened transformants of escherichia coli dh5alpha, which is sensitive to zwitterm ... | 1996 | 8763956 |
| evidence for a further enterotoxin complex produced by bacillus cereus. | out of 321 strains of bacillus cereus from several sources and isolated in four different countries, 239 (74%) produced cytotoxins. only 127 (53%) of the cytotoxic strains were positive for the b-component gene of the haemolysin bl (enterotoxin) by polymerase chain reaction (pcr). western blots using antiserum produced against enterotoxin(s) gave positive results for 199 (83%) of the cytotoxic b. cereus strains. on closer examination of seven of the strains, involved in food poisoning, we found ... | 1996 | 8768515 |
| characterisation of a non-haemolytic enterotoxin complex from bacillus cereus isolated after a foodborne outbreak. | three enterotoxic components have been isolated from a strain of bacillus cereus which was involved in a large food poisoning outbreak in norway in 1995. the components were purified by chromatography on three different columns. three proteins of 39, 45 and 105 kda, respectively, were found to be necessary for maximum cytotoxicity. the amino acid n-terminal sequences of the 39 and 45 kda proteins were determined. the 45 kda component was the same protein as the main antigen detected in the bacil ... | 1996 | 8768516 |
| the chromosome map of bacillus thuringiensis subsp. canadensis hd224 is highly similar to that of the bacillus cereus type strain atcc 14579. | a physical map of the bacillus thuringiensis subsp. canadensis hd224 chromosome based on asci, noti, and sfii restriction sites has been established. the chromosome map of 4.3 mb was similar to a revised map of the chromosome of the b. cereus type strain atcc 14579, except that the b. thuringiensis subsp. canadensis hd224 chromosome lacked a noti site and had two additional asci sites. the positions of 27 probes were identical in the common macromap. a probe for the insecticidal toxin gene, cryi ... | 1996 | 8768518 |
| [the anticomplement activity of the commercial strain of bacillus cereus ip 5832 and of enterobacteria when cocultured]. | bacillus cereus production strain ip 5832 of the preparation "bactisubtil" has been characterized with respect to its anticomplementary activity (aca) and the parameters of its heterogeneity in the process of adaptation to trypticase-soybean medium and during cultivation in anaerobic conditions have been determined. the cultivation of b.cereus strain ip 5832 jointly with escherichia coli, enterobacter cloacae and klebsiella pneumoniae strains, isolated in enteric disbiosis, affects aca in differ ... | 1996 | 8771743 |
| increased accommodation of nascent rna in a product site on rna polymerase ii during arrest. | rna polymerases encounter specific dna sites at which rna chain elongation takes place in the absence of enzyme translocation in a process called discontinuous elongation. for rna polymerase ii, at least some of these sequences also provoke transcriptional arrest where renewed rna polymerization requires elongation factor sii. recent elongation models suggest the occupancy of a site within rna polymerase that accommodates nascent rna during discontinuous elongation. here we have probed the exten ... | 1996 | 8692922 |
| seasonal variations in the isolation of salmonella typhimurium, salmonella enteritidis, bacillus cereus and clostridium perfringens from environmental samples. | calf carcasses contaminated with s. typhimurium, b. cereus and cl. perfringens were placed in either a decomposition pit or in a deep burial pit. salmonella was isolated from the soil around the decomposition pit for 27 weeks and for 15 weeks around the burial site. salmonella re-appeared in soil samples during cold winter weather after an apparent 9-week absence from the decomposition pit and after 68 weeks in the burial site (a total of 88 weeks after the start of the experiment). there was al ... | 1996 | 8693843 |
| use of site-directed mutagenesis to probe structure-function relationships of alpha-toxin from clostridium perfringens. | the nh2-terminal domain of the alpha-toxin of clostridium perfringens is highly homologous to the complete phospholipase c from bacillus cereus (pc-plc), for which a high-resolution crystal structure is available. this structural information was used as the basis of a site-directed mutagenesis strategy in which critical amino acid residues of alpha-toxin involved in zinc binding, interaction with substrate, or catalysis were replaced. biochemical studies with the corresponding toxin variants ind ... | 1996 | 8698464 |
| (all trans)-2,4,6,8,10,12-tetradecahexene-1,14-dial, a new pigment from conidiobolus paulus. | a study of the coloring matter produced by conidiobolus paulus drechsler nrrl 2648 on potato-dextrose medium led to the isolation of a new dialdehyde unsaturated metabolite, (all trans)-2,4,6,8,10,12-tetradeca-1,14-hexenedial. the structure was characterized by ms and by 1h- and 13c-nmr. the compound inhibited the growth of the gram-positive bacterium, bacillus cereus, and the gram-negative bacterium, escherichia coli (mic values of 10 micrograms and 50 micrograms, respectively). | 1996 | 8699189 |
| production of kanosamine by bacillus cereus uw85. | bacillus cereus uw85 produces two antibiotics that contribute to its ability to suppress certain plant diseases (l. silo-suh, b. lethbridge, s. j. raffel, h. he, j. clardy, and j. handelsman, appl. environ. microbiol. 60:2023-2030, 1994). to enhance the understanding of disease suppression by uw85, we determined the chemical structure, regulation, and the target range of one of the antibiotics. the antibiotic was identified as 3-amino-3-deoxy-d-glucose, also known as kanosamine. kanosamine was h ... | 1996 | 8702302 |
| inhibition by gangliosides of bacillus cereus phospholipase c activity against monolayers, micelles and bilayer vesicles. | the effect of complex glycosphingolipids (gangliosides) on the activity of phospholipase c from bacillus cereus was studied using lipid monolayers, mixed micelles and small unilamellar vesicles containing phosphatidylcholine as substrate. in all artificial membrane systems assayed, gangliosides exhibit qualitatively similar inhibitory properties. gangliosides decrease the enzyme activity irrespective of the aggregation structure in which the substrate is offered to b. cereus phospholipase c, and ... | 1996 | 8706693 |
| n-(2-ferrocene-ethyl)maleimide: a new electroactive sulphydryl-specific reagent for cysteine-containing peptides and proteins. | we report the synthesis and application of a specific electroactive label, n-(2-ferrocene-ethyl)maleimide, which provides new redox properties to organic compounds and proteins possessing sulphydryl groups. its reaction conditions with the cysteine-containing peptide, glutathione, and a terminal monooxygenase enzyme, cytochrome p450cam are presented. the labelled peptide and enzyme acquired reversible electrochemical properties due to the attached ferrocene moiety. | 1996 | 8706845 |
| microbiological quality & incidence of organisms of public health importance in food & water in ludhiana. | bacteriological analysis of 713 samples of various types of foods and related articles and potable water samples from different places in ludhiana, punjab was carried out. the highest counts ranging from 2.5 x 10(6)-7.5 x 10(8) organisms/g were observed in raw vegetables and fruits, followed by 3 x 10(6)-9.8 x 10(7)/ml, 8.3 x 10(4)-8.9 x 10(7)/g and 1 x 10(3)-6.7 x 10(7)/g in fruit juice, milk and its products, and salty/non milk snacks respectively. fresh chapati, dal, rice, cooked vegetables a ... | 1996 | 8707360 |
| [bacillus cereus chitinases: isolation and characteristics]. | three chitinases (m(r) = 68, 52 and 38 kda) have been isolated from the cultural filtrate of bacillus cereus strain vkpm b-6838 by stepwise hydrophobic chromatography on butyl-toyopearl and gel filtration on superdex 75 (fplc). the chitinases are stable in the ph range 4-10 and have the same ph optimum of activity. the 68 and 38 kda enzymes display the highest activity at 60 degrees c. while the 52 kda chitinase-at 50 degrees c. in contrast with the 68- and 52 kda enzymes, the 38 kda chitinase h ... | 1996 | 8717500 |
| genotypic and phenotypic analysis of zwittermicin a-producing strains of bacillus cereus. | many strains of bacillus cereus produce zwittermicin a, a novel antibiotic that contributes to the ability of b. cereus to suppress certain plant diseases. the purpose of this study was to identify molecular indicators of zwittermicin a production in b, cereus strains, contribute to an understanding of the ecology and evolution of this group of bacteria, and identify potential agents for control of plant disease. the fatty acid composition of 20 strains known to be zwittermicin a producers and 2 ... | 1996 | 9004505 |
| [the detection of toxinogenic bacillus cereus strains]. | investigations into optimal culture conditions for bacillus cereus in order to detect bacterial toxins in a cell culture system showed that dulbecco's modified eagle's medium supplemented with 5% fetal calf serum is the medium best suited for this purpose. the highest toxicity levels were seen when bacteria were cultured at 21 degrees c. in 42 of 43 bacillus cereus-strains isolated from milk and milk products, toxin formation was detected using the mtt-test. in 11 strains, toxin formation was ev ... | 1996 | 9012242 |
| kinetics of phosphatidylinositol-specific phospholipase c with vesicles of a thiophosphate analogue of phosphatidylinositol. | 1,2-dimyristoyloxypropane-3-thiophospho(1d-1-myo-inositol) (d-thio-dmpi) was synthesized as a substrate for the continuous spectrophotometric assay of phosphatidylinositol-specific phospholipase c (pi-plc) from bacillus cereus. release of thio-diglyceride is followed by a coupled reaction with 4,4'-dithiopyridine to produce a chromophore, 4-thiopyridine, measured by its absorption at 324 nm. sonicated vesicles of d-thio-dmpi gave sigmoidal michaelis-menten kinetics with pi-plc as a function of b ... | 1996 | 9022217 |
| [oral long-term administration of probiotic b. cereus--an alternative for the the prevention of enterotoxemia?]. | aetiology and epizootiology of enterotoxaemia, especially the connection between development of the disease and the management of feeding are described and newer knowledge about the physiopathology of the disease is presented. a comparative description of prophylactic schemes which include immuno- and chemoprophylaxis follows. the principles of the application and the effect of probiotic organisms as a new approach to prevent the disease are explained. the probiotic effect of apathogenic non-hae ... | 1996 | 9035967 |
| [formation of a resting form of bacillus cereus and micrococcus luteus]. | under certain cultivation conditions, the bacteria bacillus cereus and micrococcus luteus form cystlike refractive cells (up to 60% of the total number) that retain viability over a long time, are metabolically inactive and thermotolerant and possess specific ultrastructure. these properties allow them to be attributed to a new type of resting forms of microorganisms. | 1996 | 9102554 |
| [the detection of peptostreptococcus asaccharolyticus and bacillus cereus in clinical materials from a child who died of sepsis with a fulminant clinical course]. | a case of an acute disease with a rapid clinical course and a fatal outcome in the presence of irreversible toxicoinfectious shock, appearing in two children after the consumption of sheep kidneys, is described. the post mortem examination of the children revealed the presence of hemorrhagic, erosive and necrotic areas on the mucous membrane of the stomach, the duodenum and the upper section of the small intestine. from the material obtained by probing the stomach of one of the children 6 hours ... | 1996 | 9103069 |
| effect of high-power microwave on indicator bacteria for sterilization. | according to the superiority sterilization, a specially-designed microwave disinfector using high-power microwave energy was made. a series of sterilizing experiments have been made to determine the effect of microwave energy on several typical indicator bacteria such as bacillus subtilis var. niger, bacillus stearothermophilus, bacillus pumilus e601, staphylococcus aureus, bacillus cereus. under the conditions of different sterilization duration and unequal intensity of microwave power irradiat ... | 1996 | 9216147 |
| the role of the nonconserved residues at position 167 of class a beta-lactamases in susceptibility to mechanism-based inhibitors. | differences in specificities between the class a beta-lactamases for both substrate and inhibitors are known. the role of the nonconserved amino acid residue at position 167 of the class a enzyme, which forms a cis bond with the catalytically essential glu-166 residue, in both the hydrolysis of beta-lactam substrates and inactivation by mechanism-based inhibitors, was investigated. site-directed mutagenesis was performed on the penpc gene encoding the bacillus cereus 569/h beta-lactamase i to re ... | 1996 | 9158770 |
| [a comparative study of ethylene oxide and ionizing radiation for sterilizing bone grafts]. | to find a good way for sterilization and disinfection of bone grafts, we compared the sterilization capacity of gaseous ethylene oxide (eo) and cobalt-60 gamma radiation. the bone chips were contaminated with 10(7) bacteria per milliliter of staphylococcus aureus atcc 25923, bacillus subtilis globigii 8017 and bacillus cereus 4001, then sterilized with various doses of gaseous eo or cobalt- 60 gamma radiation. the sterilization effect of eo was more stronger and faster than that of 60co gamma ra ... | 1996 | 9594188 |
| evaluation of product analogues for purification of penicillinase by affinity chromatography. | among different matrices prepared, ampicilloic acid-polymer matrix offered 86.7% adsorption, 95% elution and 82.4% overall recovery of penicillinase. the structure of both the side chain and penicilloic or cephalosporoic acid moieties contribute to the affinity interactions. | 1996 | 9676043 |
| synthesis and kinetic evaluation of inhibitors of the phosphatidylinositol-specific phospholipase c from bacillus cereus. | substrate analogues of phosphatidylinositol (1) were synthesized and evaluated as potential inhibitors of the bacterial phosphatidylinositol-specific phospholipase c (pi-plc) from bacillus cereus. the chiral analogues of the water-soluble phospholipid substrate 5 were designed to probe the effects of varying the inositol c-2 hydroxyl group, which is generally believed to serve as the nucleophile in the first step of the hydrolysis of phosphatidylinositols by pi-plc. in the analogues 6-9, the c-2 ... | 1996 | 11667784 |
| [street-vendor foods: quality of ice creams, sherbets and sorbets sold in the urban agglomeration of antananarivo]. | a survey of selling conditions and bacteriological examinations of ice-cream was carried-out in antananarivo from june 1996 to may 1997. the way of investigation by vendors and of bacteriological examinations were widely described. sellers had classic features of a street-vended food vendor: uneducated, no having professional training and mishandling foodstuffs. 202 samples of ice-cream were collected. the contamination prevalence rate was of 95% +/- 3.7%. salmonella typhimurium was isolated fro ... | 1996 | 12463022 |
| competition as a source of errors in rapd analysis. | we have used artificial 1∶1 dna mixtures of all pairwise combinations of four doubled haploid brassica napus lines to test the ability of rapds to function as reliable dominant genetic markers. in situations where a specific rapd band is present in one homozygous line but absent in the other, the band is expected in the artificial heterozygote, i.e. in the 1∶1 dna mixture. in 84 of all 613 heterozygous situations analysed, the expected band failed to amplify in the rapd reaction. thus, rapd mark ... | 1996 | 24162529 |
| kinetic study of the active site structure of β-amylase from bacillus cereus var. mycoides. | the subsite affinities of the active site of β-amylase from bacillus cereus var. mycoides were evaluated based on hiromi's theory, using (14)c-radiolabeled maltooligosaccharides as substrate. it was estimated that the active site consisted of six subsites, and all subsite affinities could be evaluated. the active site had a common subsite arrangement with those of β -amylases from soybean and wheat bran. the intrinsic breakdown rate constant of α-1,4 glucosidic linkage (kint) was five to seven t ... | 1996 | 27281142 |
| meat from dairy cows: possible microbiological hazards and risks. | the authors provide an overview of the circumstances associated with culling of dairy cattle in the united states of america (usa) and focus on the possible significant microbiological hazards associated with meat from cull dairy cows. cull dairy cows are an important source of food in the usa, accounting for at least approximately 17% of ground beef. the potential microbiological hazards for foodborne illness from cull dairy cows discussed here include salmonella (with special attention to s. t ... | 1997 | 9501354 |
| milk pasteurisation and safety: a brief history and update. | a brief history of the development of milk pasteurisation is presented and updated. concerns about the margin of safety provided by current pasteurisation standards in terms of milk-borne pathogens such as mycobacteria (in particular mycobacterium paratuberculosis) and other emerging pathogens such as listeria monocytogenes and escherichia coli o157:h7 are discussed. with the exception of the endospores of bacillus cereus, current standards appear to be adequate for public health assurance of mi ... | 1997 | 9501358 |
| [sanitary conditions of community dining halls in greater buenos aires, argentina]. | because of the importance of food safety for public health, a study was done to determine the microbiologic quality of foods provided to patrons of community dining halls in greater buenos aires, argentina. the study was descriptive and was conducted in three municipalities with intermediate or high poverty indices; a sample of 52 dining halls was selected randomly from those municipalities. information was collected from april 1994 through april 1995 by means of surveys, direct observations, an ... | 1997 | 9445768 |
| modelling bacillus cereus growth. | the aim of this study was to model the growth, in a model system, of toxigenic strains of bacillus cereus as a function of temperature, ph and water activity. optical density (od) values were transformed into numbers of colony-forming units (cfu) by the use of a 'calibrating' relation. the growth curves were then fitted to the gompertz function which allowed the estimation of the lag-time (lambda) and the growth rate (mu). these two parameters were then modelled according to the controlling fact ... | 1997 | 9506288 |
| [a comparative study of the biological properties of biosporin and other commercial bacillus-based preparations]. | a new probiotic biosporin and other commercial biopreparations based on aerobic sporulating bacteria of the bacillus genus have been comparatively studied for their specific activity and safety. it has been established that only biosporin is characterized by expressed antagonistic activity in respect to a wide range of pathogenic and conditionally pathogenic microorganisms including those with multiple resistance to antibiotics. biosporin is also characterized by the absence of any negative acti ... | 1997 | 9511375 |
| effect of "siljo" fermentation on growth of staphylococcus aureus, bacillus cereus and listeria monocytogenes. | the fate of staphylococcus aureus, bacillus cereus, and listeria monocytogenes in control gruel and fermenting "siljo" was assessed. s. aureus reached levels of 10(8) cfu/ml within 48 h in the control gruel. in fermenting "siljo" the count decreased all through the fermentation time. b.cereus was completely inhibited in fermenting "siljo" within 24 h, whereas it survived until 72 h in the control gruel. complete inhibition of l.monocytogenes was noted at 48 h in fermenting "siljo". l.monocytogen ... | 1997 | 10214435 |
| time to b. cereus about hot chocolate. | to determine the cause of illnesses experienced by employees of a minneapolis manufacturing plant after drinking hot chocolate bought from a vending machine and to explore the prevalence of similar vending machine-related illnesses. | 1997 | 9160059 |
| mutual modulation of sphingomyelinase and phospholipase a2 activities against mixed lipid monolayers by their lipid intermediates and glycosphingolipids. | sphingomyelinase activity against pure sphingomyelin monolayers is constant up to a surface pressure of 18 mn/m and falls above it. sphingomyelinase- and phospholipase a2-mediated phosphohydrolytic pathways are mutually modulated by the presence of their respective substrates and products. at 15 mn/m non-substrate lipids such as ceramide at a mole fraction of 0.1 in mixed films with the pure substrate, inhibit the sphingomyelinase activity. ganglioside gm1, another ceramide-containing complex sp ... | 1997 | 9160338 |
| use of pulsed-field gel electrophoresis to investigate a pseudo-outbreak of bacillus cereus in a pediatric unit. | bacillus cereus is a well-known cause of food poisoning. it also causes rare systemic infections, usually in immunocompromised patients. dissemination of this species in hospitals had been reported. most of these episodes were pseudo-outbreaks and were usually secondary to equipment or environmental contamination. we report here on the use of pulsed-field gel electrophoresis (pfge) to analyze a pseudo-outbreak of b. cereus in a pediatric unit. different restriction endonucleases had been tested, ... | 1997 | 9163476 |
| bacterial viability and antibiotic susceptibility testing with sytox green nucleic acid stain. | a fluorescent nucleic acid stain that does not penetrate living cells was used to assess the integrity of the plasma membranes of bacteria. sytox green nucleic acid stain is an unsymmetrical cyanine dye with three positive charges that is completely excluded from live eukaryotic and prokaryotic cells. binding of sytox green stain to nucleic acids resulted in a > 500-fold enhancement in fluorescence emission (absorption and emission maxima at 502 and 523 nm, respectively), rendering bacteria with ... | 1997 | 9172364 |
| [bacillus cereus pneumonia after thoracic trauma. case report and review of the literature]. | we report on the case of a pneumonia caused by bacillus cereus in a patient who sustained severe thoracic trauma, fractures of ribs 3-12, left lung contusion and haematopneumothorax. bronchoscopic alveolar lavage (bal) performed on icu day four helped identifying the underlying cause of pneumonia. inspite of early diagnosis and antibiotic treatment with clindamycin, cefotaxim and tobramycin, which rapidly eliminated the bacteria from the patient's lungs, the patient eventually died from the seve ... | 1997 | 9172719 |
| cloning, sequencing and overexpression of the leucine dehydrogenase gene from bacillus cereus. | the l-leucine dehydrogenase gene from bacillus cereus (dsm 626) was cloned from a partial genomic library and sequenced. the open reading frame has 1101 bp and codes for a protein of 39.9 kda. the deduced amino acid sequence of the leudh from b. cereus shares 70-80% identity with leudh's from the thermophilic strains b. stearothermophilus and thermoactinomyces intermedius. the active protein was overexpressed in escherichia coli to yield approximately 30% of the total soluble protein. | 1997 | 9188201 |
| intracellular transport and processing of protein toxins produced by enteric bacteria. | bacterial toxins are associated with disease in humans and animals. toxins can either be preformed in food or produced by bacteria in the intestine. there are two types of toxins: heat-labile protein toxins and heat stabile toxins. heat labile toxins are produced by bacillus cereus, clostridium perfringens, escherichia coli, and vibrio cholerae, and heat-stabile enterotoxins consisting of relatively few amino acids are produced by escherichia coli and acts by activation of guanylate cyclase. sim ... | 1997 | 9192018 |
| the refined crystal structure of bacillus cereus oligo-1,6-glucosidase at 2.0 a resolution: structural characterization of proline-substitution sites for protein thermostabilization. | the crystal structure of oligo-1,6-glucosidase (dextrin 6-alpha-glucanohydrolase, ec 3.2.1.10) from bacillus cereus atcc7064 has been refined to 2.0 a resolution with an r-factor of 19.6% for 43,328 reflections. the final model contains 4646 protein atoms and 221 ordered water molecules with respective root-mean-square deviations of 0.015 a for bond lengths and of 3.166 degrees for bond angles from the ideal values. the structure consists of three domains: the n-terminal domain (residues 1 to 10 ... | 1997 | 9193006 |
| [meningoencephalitis caused by bacillus cereus]. | 1997 | 9198505 | |
| the use of bacillus diarrhoeal enterotoxin (bde) detection using an elisa technique in the confirmation of the aetiology of bacillus-mediated diarrhoea. | a commercially available elisa kit was used for the detection of bacillus diarrhoeal enterotoxin (bde) in a variety of foods and faeces. the ability of isolates of bacillus spp., including bacillus cereus, to produce bde in brain heart infusion broth containing 0.1% glucose was also checked by use of the kit. results show that 29 out of 31 b. cereus isolates were enterotoxigenic. foods positive for performed bde were always contaminated with > 10(5) b. cereus cfu g-1, but not all foods contamina ... | 1997 | 9202434 |
| a universal protocol for pcr detection of 13 species of foodborne pathogens in foods. | a universal protocol for pcr detection of 13 species of foodborne pathogens in foods was developed. the protocol used a universal culture medium and the same pcr conditions with 13 sets of specific primers. the 13 species of foodborne pathogens examined were escherichia coli, e. coli-etec, e. coli-o157:h7, shigella spp., salmonella spp., yersinia enterocolitica, y. pseudotuberculosis, vibrio cholerae, v. parahaemolyticus, v. vulnificus, listeria monocytogenes, staphylococcus aureus and bacillus ... | 1997 | 9449811 |
| ventricular shunt infection and meningitis due to bacillus cereus. | non-anthrax bacillus species are usually considered to be contaminants if found in clinical specimens. only a few patients with systemic infections due to bacillus cereus are reported. we present the case of a 18-month old boy with a primitive neuroectodermal tumor (pnet) in the brainstem and obstructive hydrocephalus that required an outlying and subsequently a ventriculoperitoneal drain. following contamination at the site of entry of the external drain, shunt infection and meningitis with bac ... | 1997 | 9453032 |
| [transmembrane potential and atp-ase activity of the plasma membrane of bacteria exposed to heavy metals]. | effect of heavy metals (gold, zinc and cadmium) on the atpase activity and transmembrane potential (delta psi) as integral index of bacterial cells membrane processes intensity has been studied. it was shown that studied membrane systems of bacteria are very sensitive to the action of heavy metals and they can serve as indicators of resistance of bacterial cultures. | 1997 | 9454379 |
| bacillus cereus and orthopaedic surgical wound infection associated with incontinence pads manufactured from virgin wood pulp. | 1997 | 9457613 | |
| mutagenesis study of the glycosylphosphatidylinositol phospholipase c of trypanosoma brucei. | the glycosylphosphatidylinositol phospholipase c (gpi-plc) from trypanosoma brucei is particularly effective in hydrolysing the gpi-anchors of some proteins. the enzyme is inhibited by zn2+ and p-chloromercurylphenylsulphonic acid, both of which can act as sulphydryl reagents, suggesting that a cysteine residue may be important in catalysis. single cysteine to serine mutants have been produced for all eight cysteines in gpi-plc; all the mutants were fully active in vitro and were still susceptib ... | 1997 | 9476790 |
| fatal meningoencephalitis due to bacillus anthracis. | we report the first case of fatal anthrax meningoencephalitis in hong kong over the past 60 years. a 13 year-old boy presented with right lower quadrant pain, diarrhoea and progressive headache. lumbar puncture yielded gram positive bacilli initially thought to be bacillus cereus, a contaminant. he was treated with ampicillin and cefotaxime, but died 3 days after hospitalization. the organism isolated from blood and cerebrospinal fluid was later identified as bacillus anthracis. | 1997 | 9484689 |
| [severe systemic infections with bacillus cereus: current aspects of the pathogenicity of the genus bacillus]. | 1997 | 9488753 | |
| beta-amylase production by some bacillus cereus, bacillus megaterium and bacillus polymyxa [correction of polymaxa] strains. | the production of extracellular beta-amylase by some bacillus cereus, bacillus megaterium and bacillus polymyxa [corrected] strains was investigated, and the maximal yields of the enzyme were 3.6; 9.3 and 20.4 u/ml of the culture fluid, respectively (u, 1 mumol of maltose equivalent per min at 30 degrees c). several cultivation media were used for beta-amylase production. bacillus cereus and some strains of bacillus megaterium gave good yields of beta-amylase only in medium with the addition of ... | 1997 | 9516983 |
| precursor-directed biosynthesis of erythromycin analogs by an engineered polyketide synthase. | a genetic block was introduced in the first condensation step of the polyketide biosynthetic pathway that leads to the formation of 6-deoxyerythronolide b (6-deb), the macrocyclic precursor of erythromycin. exogenous addition of designed synthetic molecules to small-scale cultures of this null mutant resulted in highly selective multimilligram production of unnatural polyketides, including aromatic and ring-expanded variants of 6-deb. unexpected incorporation patterns were observed, illustrating ... | 1997 | 9219693 |
| acquired carbapenemases. | 1997 | 9222034 | |
| in-vitro activity of trovafloxacin against clinical bacterial isolates from patients with cancer. | the antibacterial activity of trovafloxacin was compared with that of ciprofloxacin, levofloxacin, ofloxacin, sparfloxacin and norfloxacin against bacterial isolates from patients with cancer. in general, the activity of trovafloxacin was comparable to that of ciprofloxacin, levofloxacin and sparfloxacin against most gram-negative isolates tested (minor differences in the activity of each agent against individual species were seen) and it was the most active agent tested against stenotrophomonas ... | 1997 | 9222065 |
| channelling of deoxyribose moiety of exogenous dna into carbohydrate metabolism: role of deoxyriboaldolase. | in bacteria, the addition of (deoxy)nucleosides or (deoxy)ribose to the growth medium causes induction of enzymes involved in their catabolism, leading to the utilisation of the pentose moiety as carbon and energy source. in this respect, deoxyriboaldolase appears the key enzyme, allowing the utilisation of deoxyribose 5-p through glycolysis. we observed that not only deoxynucleosides, but also dna added to the growth medium of bacillus cereus induced deoxyriboaldolase; furthermore, the switch o ... | 1997 | 9226884 |