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prevalence and genetic characterization of vibrio vulnificus in raw seafood and seawater in malaysia.vibrio vulnificus is a highly invasive human pathogen that exists naturally in estuarine environment and coastal waters. in this study, we used different pcr assays to detect v. vulnificus in 260 seafood and 80 seawater samples. v. vulnificus was present in about 34 (13%) of the 260 seafood samples and 18 (23%) of the 80 seawater samples. repetitive extragenic palindromic pcr (rep-pcr) and enterobacterial repetitive intergenic consensus pcr (eric-pcr) were applied to subtype the v. vulnificus is ...201324112583
[preparation of monoclonal antibodies against flagellin core protein of vibrio vulnificus and its application in testing vibrio vulnificus from food products].to prepare the monoclonal antibodies (mabs) against flagellin core protein of vibrio (v.) vulnificus and establish the double-antibody sandwich elisa for testing v. vulnificus from food products.201324103267
role of capsular polysaccharide (cps) in biofilm formation and regulation of cps production by quorum-sensing in vibrio vulnificus.extracellular polysaccharides, such as lipopolysaccharide and loosely associated exopolysaccharides, are essential for vibrio vulnificus to form biofilms. the role of another major component of the v. vulnificus extracellular matrix, capsular polysaccharide (cps), which contributes to colony opacity, has been characterized in biofilm formation. a cps-deficient mutant, whose wbpp gene encoding udp-glcnac c4-epimerase was knocked out, formed significantly more biofilm than wild type, due to increa ...201324102883
transcriptomic analysis of genes modulated by cyclo(l-phenylalanine-l-proline) in vibrio vulnificus.diketopiperazine is produced by various organisms, including bacteria, fungi, and animals, and has been suggested as a novel signal molecule involved in the modulation of genes with various biological functions. vibrio vulnificus, which causes septicemia in humans, produces cyclo(l-phenylalanine-l-proline) (cfp). to understand the biological roles of cfp, the effect of the compound on the expression of the total mrna in v. vulnificus was assessed by nextgeneration sequencing. based on the transc ...201324100622
rapid emergency medicine score as a main predictor of mortality in vibrio vulnificus-related patients.vibrio vulnificus causes potentially life-threatening and rapidly progressing infections. therefore, the severity-of-illness assessment appears to be important for v vulnificus-infected patients at the time of admission. the aim of our study was to evaluate the performance of the severity-of-illness scoring model on admission in v vulnificus-infected patients.201323702054
a novel agar formulation for isolation and direct enumeration of vibrio vulnificus from oyster tissue.a new selective and differential medium, vibrio vulnificus x-gal (vvx), was developed for direct enumeration of v. vulnificus (vv) from oyster samples. this agar utilizes cellobiose and lactose as carbon sources, and the antibiotics colistin and polymyxin b as selective agents. hydrolysis of 5-bromo-4-chloro-3-indolyl- beta-d-galactopyranoside (x-gal), used in the agar as a lactose analog, produces an insoluble blue dye that makes lactose positive colonies easily distinguishable from any non-lac ...201323660708
comparative analysis of induction of osmotic-stress-dependent genes in vibrio vulnificus exposed to hyper- and hypo-osmotic stress.vibrio vulnificus, a halophilic pathogenic bacterium of marine environments, encounters changes in salinity in its natural habitat and in the food-processing environment. the comparative response of v. vulnificus to hyperosmotic and hypoosmotic stress in terms of gene expression was investigated. genes belonging to the prou operon for transport of compatible solutes and compatible solute synthesis were significantly upregulated (3- to 4.7-fold) under hyperosmotic stress. under hypoosmotic stress ...201323647346
genome sequence of the pathogenic bacterium vibrio vulnificus biotype 3.we report the first genome sequence of the pathogenic vibrio vulnificus biotype 3. this draft genome sequence of the environmental strain vvyb1(bt3), isolated in israel, provides a representation of this newly emerged clonal group, which reveals higher similarity to the clinical strains of biotype 1 than to the environmental ones.201323599289
a dual regulatory role of cyclic adenosine monophosphate receptor protein in various virulence traits of vibrio vulnificus.vibrio vulnificus causes fatal septicemia in susceptible subjects after the ingestion of raw seafood. in the present study, the roles of cyclic adenosine monophosphate (camp) receptor protein (crp) in v. vulnificus pathogenesis were investigated. a mutation in the v. vulnificus crp gene resulted in a significant down-regulation of various virulence phenotypes, except for rtxa1-mediated cytoskeletal rearrangement. bacterial growth was impeded by the crp mutation. in addition, colony morphology wa ...201323586631
a new culture-based method for the improved identification of vibrio vulnificus from environmental samples, reducing the need for molecular confirmation.vibrio vulnificus is an opportunistic human pathogen responsible for 95% of seafood related deaths in the us. monitoring the presence of this bacterium in estuarine waters and shellfish is of medical and economic importance due to its ability to cause severe wound infections and fulminant septicemia. current methods for isolating v. vulnificus from environmental samples typically employ an initial selective medium which requires subsequent molecular confirmation of presumptive v. vulnificus isol ...201323566825
regulation of haemolysin (vvha) production by ferric uptake regulator (fur) in vibrio vulnificus: repression of vvha transcription by fur and proteolysis of vvha by fur-repressive exoproteases.vvha produced by vibrio vulnificus exhibits cytolytic activity to human cells including erythrocytes. since haemolysis by vvha may provide iron for bacterial growth and pathogenicity, we investigated the expression of vvha to elucidate the regulatory roles of fur, a major transcription factor controlling iron-homeostasis. fur repressed the transcription of vvhba operon via binding to the promoter region. however, haemolysin content and haemolytic activity were lowered in cell-free supernatant of ...201323560801
genotypic diversity and virulence characteristics of clinical and environmental vibrio vulnificus isolates from the baltic sea region.the genetic diversity of vibrio vulnificus isolates from clinical and environmental sources originating from the baltic sea region was evaluated by multilocus sequence typing (mlst), and possible relationships between mlst clusters, potential genotypic and phenotypic traits associated with pathogenicity, and source of isolation were investigated. the studied traits included genotyping of polymorphic loci (16s rrna, vcg, and pilf), presence/absence of potential virulence genes, including nana, na ...201323542621
computational, structural, and kinetic evidence that vibrio vulnificus frsa is not a cofactor-independent pyruvate decarboxylase.the fermentation-respiration switch (frsa) protein in vibrio vulnificus was recently reported to catalyze the cofactor-independent decarboxylation of pyruvate. we now report quantum mechanical/molecular mechenical calculations that examine the energetics of c-c bond cleavage for a pyruvate molecule bound within the putative active site of frsa. these calculations suggest that the barrier to c-c bond cleavage in the bound substrate is 28 kcal/mol, which is similar to that estimated for the uncata ...201323452154
[necrotising fasciitis caused by vibrio vulnificus]. 201323433727
impact of timing of surgery on outcome of vibrio vulnificus-related necrotizing fasciitis.the aim of this study was to evaluate the impact of timing of surgery on mortality risk in patients with necrotizing fasciitis (nf) caused by vibrio vulnificus infection.201323414632
distribution of virulence markers among vibrio vulnificus isolates of clinical and environmental origin and regional characteristics in japan.vibrio vulnificus is an opportunistic human pathogen that is widely distributed in estuarine environments and is capable of causing necrotizing fasciitis and sepsis. in japan, based on epidemiological research, the incidences of v. vulnificus were concentrated in kyusyu, mainly in coastal areas of the ariake sea. to examine the virulence potential, various genotyping methods have recently been developed. this study aimed to investigate the distribution of virulence markers among v. vulnificus is ...201323383115
presence of virulence markers in environmental vibrio vulnificus strains.this work aims to demonstrate the presence of several genes and factors associated with virulence in strains isolated from the environment at pueblo viejo lagoon, state of veracruz, mexico.201323351134
accurate diagnosis and treatment of vibrio vulnificus infection: a retrospective study of 12 cases.vibrio vulnificus causes an infectious disease that has extremely poor convalescence and leads to necrotic fasciitis. in this study, we sought to define the characteristic epidemiology of v. vulnificus infection and clarify its diagnosis at the global level.201323332442
structural and biochemical characterization of an atypical short-chain dehydrogenase/reductase reveals an unusual cofactor preference.short-chain dehydrogenases/reductases (sdrs) encompass a large and functionally diverse family of enzymes with representative members in all kingdoms of life. despite the wealth of reactions catalyzed by sdrs, they operate through a well-conserved and efficient reaction mechanism centered in a conserved catalytic tetrad (asn-ser-tyr-lys) and the employment of an appropriate cofactor. in recent years, sdrs that lack the signature catalytic tetrad have been identified, thus adding a perplexing twi ...201323311896
the interactions of vibrio vulnificus and the oyster crassostrea virginica.the human bacterial pathogen, vibrio vulnificus, is found in brackish waters and is concentrated by filter-feeding molluscan shellfish, especially oysters, which inhabit those waters. ingestion of raw or undercooked oysters containing virulent strains of v. vulnificus can result in rapid septicemia and death in 50 % of victims. this review summarizes the current knowledge of the environmental interactions between these two organisms, including the effects of salinity and temperature on colonizat ...201323280497
detection and differentiation of vibrio vulnificus and v. sinaloensis in water and oysters of a gulf of mexico estuary.vibrio vulnificus is a potentially lethal human pathogen that occurs naturally in estuarine waters and shellfish. vibrio vulnificus was quantified in water and oysters from florida's gulf coast by plating on mcpc agar, enrichment and plating, and quantitative pcr (qpcr). vibrio vulnificus was detected in 19%, 29%, and 97% of samples respectively by direct plating, qpcr, and enrichment. only 8% of typical colonies from direct plating were confirmed by pcr for vvha; others yielded no or atypically ...201323240813
inhibition of binding of vibrio vulnificus hemolysin (vvh) by mβcd.vibrio vulnificus secrets a pore-forming toxin called vibrio vulnificus hemolysin (vvh). in this study, we showed that methyl-beta-cyclodextrin (mβcd), an oligosaccharide, decreased binding of vvh to chinese hamster ovary (cho) cells, resulting in inhibition of its cytotoxicity. when the vvh was incubated with mβcd, cytotoxicity of the toxin was inhibited from 100.3 ± 7.2% to 19.6 ± 5.3%. binding analysis showed that the amount of vvh on the cells was decreased from 101.4 ± 9.2% to 18.1 ± 8.0% o ...201323238452
fatal vibrio vulnificus sepsis in vertically acquired hepatitis c. 201322367339
preventing complications for the toxic shock syndrome patient. 20133848384
confidentiality and the medical report. 20133831650
the role of androstenedione and testosterone in the reproduction and antler growth of a male white-tailed deer.seasonal levels of androstenedione and testosterone were investigated in plasma of mature intact and castrated male white-tailed deer. in four intact bucks, androstenedione concentrations were low in february and march (around 1 nmol/l) and then increased significantly (p less than 0.05) from april to november (peak 2.34 nmol/l). testosterone remained low (below 3.5 nmol/l) from february to august and then rose significantly (p less than 0.01) till november (peak 36.78 nmol/l). both hormones dec ...20133811770
local anaesthetics. iv--synthesis and activity of 2-(n-substituted or n,n-disubstituted aminoacetamido)-4- or -4,5-substituted thiazoles.synthesis is described of eleven new 2-(n-substituted or n,n-disubstituted aminoacetamido)-4- or -4,5-substituted thiazoles (i). their hydrochlorides were screened for local anaesthetic activity on frogs by the sciatic plexus method and the activity compared with that of procaine.20133792539
[incidence of secondary hypertension]. 20133960533
[incidence of secondary hypertension]. 20133960533
the role of the paediatric endoscopist.the role of the paediatric endoscopist has been a changing one. when i first commenced at the royal alexandra hospital for children in sydney 25 years ago, paediatric endoscopy of the upper respiratory tract and oesophagus was a limited and poorly defined field. most endoscopic examinations were requested, often reluctantly, by a paediatrician in the investigation of stridor. in fact my first consultation involved instructions to perform a laryngoscopy, but not a bronchoscopy and general anaesth ...20133805880
[in vitro fertilization in reduced male fertility]. 20134029742
[factors determining the severity of traumatic lesions of the popliteal artery]. 20134073583
insight into the evolution of vibrio vulnificus biotype 3's genome.vibrio vulnificus is an aquatic bacterium and an important human pathogen. strains of v. vulnificus are biochemically classified into three biotypes. the newly emerged biotype 3 appears to be rather clonal and geographically restricted to israel, where it caused an outbreak of wound infections and bacteremia. to understand the evolution of the bacterium's genome, we sequenced and analyzed the genome of biotype 3 strain vvyb1(bt3), and then conducted a microbial environmental survey of the hypoth ...201324385974
[troublesome teeth in ambulatory care]. 20133859858
[troublesome teeth in ambulatory care]. 20133859858
correlation of double-contrast high-density barium enema, colonoscopy, and histology in children with special attention to disparities.colonoscopic and double-contrast high-density barium enema (dcbe) findings were correlated in 68 patients (39 boys and 29 girls) aged 6 months to 18 years (mean 11.6 years) evaluated over a 24-month period. there was excellent correlation in 53 patients (78.0%) and good correlation in another 3 (4.4%) who had identical diagnoses and only slightly differing extent of disease reported. in 2 of these, dcbe showed more extensive disease, confirmed histologically in 1. distal colitis seen on colonosc ...20133725447
a new species of the early tertiary rodent thisbemys. 20133716069
[conservative treatment in splenic injuries. results of a follow-up in patients with autotransplants]. 20133725057
projecting mri use: key to scanner purchases. 20133679125
peroxidase-based colorimetric determination of l-ascorbic acid. 20133592224
[analysis of hba1 is necessary in the routine check-up of patients with type 2 diabetes mellitus]. 20133807486
[role of foci of chronic infection of orl organs in the pathogenesis of neurodermatitis]. 20133811584
[development of esophageal cancers in patients following cardiomyotomy].during a 20 year period we observed that in six patients with carcinoma of the esophagus associated with achalasia, four patients had had a prior heller operation. fifty patients with achalasia of the esophagus took part in endoscopic and histologic surveillance 5-15 years after cardiomyotomy. we have found a correlation between the severity of histologic changes and the duration of symptoms before the operation. it seems reasonable to advise early therapy to avoid the development of a dilated, ...20133807499
[sleep in children]. 20134321695
temporal lobe epilepsy with schizophrenia.i have reported a case of coexisting schizophrenia and complex partial seizures to augment earlier reports of schizophrenia and psychomotor seizures masquerading as one another. i have attempted to clarify the differential diagnosis and reinforce the need to do thorough studies in such cases.20133616704
[significance of internalization and externalization in normal development]. 20133685240
phantom testing of peripheral artery. absolute blood flow measurement with digital arteriography.a technique to measure absolute arterial blood flow in ml/min has been developed utilizing software programs with high-speed digital recording for the off-line analysis of intra-arterial injections of contrast medium. measurements of pulsatile flow in a phantom for a physical flow model showed that calculations with the final upgrades were within 10% of known flow, using a recording rate of 30 frames/s, a diameter tubing of 5 mm, and flow rates of 300 to 400 ml/min. quantitative absolute flow of ...20133886590
alcohol-related symptoms. 20133877581
treatment of acute abscesses by incision, curettage and primary suture without antibiotics: a controlled clinical trial.one hundred and thirty-seven consecutive acute superficial abscesses were randomly allocated to incision, curettage and primary suture without parenteral antibiotics (64 cases) or to conventional treatment with incision, drainage and dressings (73 cases). wound healing was faster; 7.0 days: 25.1 days (p less than 0.01, wilcoxon's rank sum test), the number of hospital visits was smaller; 3.8 visits: 11.1 visits (p less than 0.01) and the time off work was shorter; 4.0 days: 14.1 days (p less tha ...20133881155
vasodilator drugs in patients with chronic ischaemic heart failure.the effects of intravenous nitroglycerin (ntg), trimetaphan (tmp), and phentolamine (ptl) on pulmonary artery diastolic pressure (padp), systemic arterial pressure (sap), cardiac index (ci) and systemic vascular resistance (svr) in patients (12 in each treated group) with chronic ischaemic heart failure are analyzed. each group was divided into two subgroups according to the initial padp taking into account the mean value in the whole group. a significant decrease in padp (by 40%; p less than 0. ...20133935374
[the significance of abnormal qrs amplitudes in the electrocardiogram]. 20134222444
structure and organization of immunoglobulin vh genes in heterodontus, a phylogenetically primitive shark. 20133936736
structure and organization of immunoglobulin vh genes in heterodontus, a phylogenetically primitive shark. 20133936736
emotions and obesity among mexican-american children.the purpose of this study was to determine what relationships, if any, existed between high levels of body fat and emotional motivations for eating among mexican-american children. subjects (n = 177) were drawn from children aged 10-14 years who participated in the national youth sports program in the lower rio grande valley region of texas. data were gathered through a self-report instrument dealing with emotional motivators involved in eating and through individual anthropometric measurements. ...20133845258
associated postural adjustments in parkinson's disease.postural activity in muscles of the back and legs associated with voluntary forward elevation of the arm at the shoulder with the subject standing was examined in 14 patients with parkinson's disease and nine normal individuals. the time of onset of emg activity in each of the postural muscles, relative to the onset of activity in the prime mover, was the same for both patient and normal groups and did not depend on load. the amplitude of the emg bursts and their frequency of occurrence, was les ...20133806114
[urinary acidic mucopolysaccharides in normal individuals and patients with hurler's syndrome]. 20134240974
[urinary acidic mucopolysaccharides in normal individuals and patients with hurler's syndrome]. 20134240974
wheelchair use by ambulatory nursing home residents.in a survey of wheelchair use in two nursing homes, the authors found a substantial number of cognitively intact nursing home residents who walked, but who also used a wheelchair. using chart review and interviews with the residents, it was found that multiple physical factors, including pain, strength, endurance, vision and balance, and multiple social and environmental factors, including the fear of falling, were related to the resident's decision to use a wheelchair as an alternative means of ...20133782699
[acute myocardial infarction during fasting]. 20133765158
a computer model of neck muscle endurance and fatigue as a function of helmet loading.a series of experiments were conducted in which the neck muscles of volunteer subjects were dynamically and statistically loaded by systematic variations of twenty-four headgear configurations consisting of eight different centers-of-gravity (cgs) times three different weights. six subjects would rotate their heads laterally (from side-to-side) for 30 min with each of the headgear loading combinations. immediately thereafter, the subject would position his head in an isometric head dynamometer a ...20133754195
mri: applications in soft tissue anatomy. 20133739418
the cell-to-cell channel of gap junctions. 20133815521
the dorsal approach for internal fixation of fractures of the lateral malleolus.anatomic reduction and internal fixation of the lateral malleolus are the key to management of ankle fractures. the usual application of one-third tubular plates to the lateral surface of the distal fibula has certain disadvantages. in selected cases we prefer a dorsal approach to the lateral malleolus. indications, technique and experience are discussed.20133725110
measurement of sebum output using a lipid absorbent tape.a sebum absorbent tape is introduced as a reproducible and convenient method for estimation of sebaceous gland output. we have tested the reproducibility of this method by serial measurements of sebum excretion rates (ser) of 10 individuals over a 6-week period, and in addition we have correlated this method with the conventional hexane extraction technique. the sebum absorbent tapes gave consistent values for the sers, and within subjects variation over the 6-week period was statistically nonsi ...20133734473
self-resistance in actinomycetes producing inhibitors of rna polymerase. 20133710921
[effect of irradiation on the size of isolated liver mitochondria].the role of external effects in the time course of changes in hepatocyte mitochondria size was studied. suspensions of mitochondria isolated from the liver of inbred mice were investigated at various periods after gamma irradiation. statistical analysis of changes in the size of isolated mitochondria has shown that irradiation leads to an increase in mitochondria size, reflected in the distribution pattern. the changes may be related to the functional activity, and distribution shifts may be reg ...20133697486
metabolism during the postinjury period. 20134906806
error rates in prenatal cystic fibrosis diagnosis. 20133713828
clinical, morphologic, biochemical, and immunohistochemical aspects of dialysis-associated amyloidosis. 20133675951
[diagnostic usefulness in the measurement of crp by lpia-300]. 20133669370
[clinical considerations on post-traumatic vasomotor disorders (sudeck's atrophy)]. 20134170453
[treatment with cis-platin. retinal toxic effects]. 20133603502
[developmental course of opiate-dependent patients over 6 years (1979-1985)].drug addicts of the basle area have been analysed during 6 years in connexion with the national research programme 'social integration' and also a following project. the results are contradictory to the pessimism which exists against changes of development of drug addicts.20133630400
[a simple method of measuring serum sialic acid level as a neoplasm marker]. 20133684762
long term effect of gynecologic age on somatic growth of children.a sample of new orleans black primiparous women under 25 years of age, participants in the national collaborative perinatal study, and their singleton children were studied to determine the relationship of maternal gestational age (gea), gynecologic age (gya) and size for gea (s/gea) on the child's growth over seven years observation. early childbearers (less than 4 years gya) had a somewhat higher proportion of preterm births than the late group (greater than or equal to 4 yrs gya). a repeated ...20133624531
isolated perfused ambystoma proximal tubule: hydrodynamics modulates ultrastructure.a method using a pressure-sensing servo-pipette is described for measuring downstream transepithelial pressure within isolated renal tubules perfused at flow rates designed to keep luminal solution composition constant. the hydrodynamics of in vitro microperfusion of isolated proximal tubules of ambystoma tigrinum was varied and different states of transepithelial hydrostatic pressure difference, axial tubule flow, and transepithelial transport were correlated with epithelial ultrastructure. tub ...20133591955
you and malpractice stress: v. a death in the family. 20133559428
modifications of plasma lipoproteins after lipase activation in patients with chylomicronemia.lipoprotein lipase (lpl) and hepatic lipase (hl) are enzymatic activities involved in lipoprotein metabolism. the purpose of this study was to analyze the physicochemical modifications of plasma lipoproteins produced by lpl activation in two patients with apoc-ii deficiency syndrome and by hl activation in two patients with lpl deficiency. lpl activation was achieved by the infusion of normal plasma containing apoc-ii and hl was released by the injection of heparin. lipoproteins were analyzed by ...20133559389
spontaneous passage of renal calculi through nephrocutaneous fistula due to calculous pyelonephritis. 20133567499
[sketches on the history of the training of paramedical personnel in russia]. 20133553835
[lenin's "iskra" on the role and health status of adolescent workers in russia]. 20133523773
free radicals of biological interest. ii. electron spin resonance (esr) spectra of 2537 a irradiated amino acids. 20144295529
free radicals of biological interest. ii. electron spin resonance (esr) spectra of 2537 a irradiated amino acids. 20144295529
[ultrastructural characteristics of lamellar exfoliation of the newborn. report of 2 cases]. 20144615293
[qualitative cytology of the cerebrospinal fluid in primary cerebral atrophy]. 20144249221
psychological impact of acne in adolescence. 20144239357
full mouth restoration under general anesthesia in pedodontic practice. 20144229229
the value of unionization to faculty. 20144492430
the value of unionization to faculty. 20144492430
[fascial fat herniation causing backache]. 20144223461
simplified methods for automated ion-exchange separation of peptides and accelerated manual edman degradations. 20144202981
[red noses (author's transl)]. 20144282887
[use of the levin method for laboratory diagnosis of liver cirrhosis]. 20144183885
the role of radiology in dentistry for children. 20144225584
changes of the sella turcica in chromophobic adenomas and eosinophilic adenomas. 20144286984
the prevention of dental caries by mouthrinsing with solutions of neutral sodium fluoride. 20144149260
control of diesel exhaust odors. 20144138799
[the cause of volkmann's contracture--a primary neurotrophic complication of injuries in children]. 20144188995
development and application of a model for estimating alveolar and interstitial dust levels. 20144091370
observations in petit mal status. 20144166754
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