Publications

a human y-chromosome specific repeated dna family (dyz1) consists of a tandem array of pentanucleotides.	we have determined the complete nucleotide sequence of a 3564 bp ecori fragment which represents a major component of the human y-chromosome specific repeated dna family (dyz1). sequencing result showed a tandem array of pentanucleotides after five nucleotides were inserted or deleted at four positions. 229 out of the 713 pentanucleotides were ttcca, and 297 were its single-base substituents. southern hybridization analyses of male genomic dnas showed that several endonuclease cleavage sites wer ...	1986	3774538
effects of sex steroids on the development of two granule cell subpopulations in the rat accessory olfactory bulb.	the effects of postnatal male castration on day 1 (d1) after birth and female androgenization on accessory olfactory bulb (aob) light and dark granule cell populations were studied. control males showed a greater number of both light and dark aob granule cells than females. postnatal treatment reversed these differences in the light granule cells. female androgenization on d1 does not affect the number of aob dark granule cells. however, male orchidectomy also on d1 significantly decreases the n ...	1986	3779445
gangliosides and synaptosomal calcium homeostasis.	in this study, depolarization-dependent (net) calcium transport and binding capacity to rat brain synaptosomes was investigated under conditions affecting the ganglioside composition of the synaptoplasmatic membranes. specific interaction of the monosialoganglioside gm1 with the membrane, as observed in low (40 nm) concentration of ganglioside, resulted in significant inhibition of the maximal depolarization-induced calcium uptake. the changes in ganglioside composition of synaptosomes produced ...	1986	3781769
the mammalian beta-tubulin repertoire: hematopoietic expression of a novel, heterologous beta-tubulin isotype.	we describe the structure of a novel and unusually heterologous beta-tubulin isotype (m beta 1) isolated from a mouse bone marrow cdna library, and a second isotype (m beta 3) isolated from a mouse testis cdna library. comparison of m beta 1 and m beta 3 with the completed (m beta 4, m beta 5) or extended (m beta 2) sequence of three previously described beta-tubulin isotypes shows that each includes a distinctive carboxy-terminal region, in addition to multiple amino acid substitutions througho ...	1986	3782288
the mammalian beta-tubulin repertoire: hematopoietic expression of a novel, heterologous beta-tubulin isotype.	we describe the structure of a novel and unusually heterologous beta-tubulin isotype (m beta 1) isolated from a mouse bone marrow cdna library, and a second isotype (m beta 3) isolated from a mouse testis cdna library. comparison of m beta 1 and m beta 3 with the completed (m beta 4, m beta 5) or extended (m beta 2) sequence of three previously described beta-tubulin isotypes shows that each includes a distinctive carboxy-terminal region, in addition to multiple amino acid substitutions througho ...	1986	3782288
[the effect of the alkaloid narcotine and its diols on tissue histamine levels].		1986	3786390
estimation of regional gas and tissue volumes of the lung in supine man using computed tomography.	this study was intended to discover how well computed tomography could recover the volume and weight of lung like foams in a body like shell, and then how well it could recover the volume and weight of the lungs in supine man. model thoraces were made with various loaves of bread submerged in water. computed tomography scans recovered the volume of the model lungs (true volume range 250-12,500 ml) within +0.2 (sd 68) ml and their weights (true range 72-3125 g) within +30 (78) g. scans also recov ...	1986	3787544
methylhistidine changes in tibialis anterior muscles of 6-mercaptopurine-treated rats.		1986	3787652
failure of norfloxacin to eradicate chlamydia trachomatis in nongonococcal urethritis.	norfloxacin has some activity in vitro against chlamydia trachomatis and ureaplasma urealyticum, although not at levels attainable in serum. in this study, norfloxacin was administered (400 mg orally twice daily for 10 days) to men with acute nongonococcal urethritis. of 25 men from whom c. trachomatis was initially isolated, 21 had the organism reisolated at the first follow-up visit posttreatment, and there were minimal changes in the number of inclusion-forming units in culture. ultimately, a ...	1986	3789692
frequency of four classes of tetracycline resistance determinants in salmonella and shigella spp. clinical isolates.	the frequencies of tetracycline resistance determinants of the classes a, b, c, and d were determined in 53 non-lactose-fermenting clinical isolates. the most frequent class of determinant in salmonella typhimurium and shigella flexneri strains was class b; however, the predominant determinant in shigella sonnei strains was class c.	1986	3789700
glucose regulation of cephamycin biosynthesis in streptomyces lactamdurans is exerted on the formation of alpha-aminoadipyl-cysteinyl-valine and deacetoxycephalosporin c synthase.	glucose exerted a concentration-dependent negative regulation on the biosynthesis of cephamycin c by streptomyces lactamdurans. formation of the cephamycin precursor delta(alpha-aminoadipyl)-cysteinyl-valine was greatly decreased by excess glucose. the ring-expanding enzyme deacetoxycephalosporin c synthase was strongly repressed by glucose in vivo. isopenicillin n synthase (cyclase) and isopenicillin n epimerase were not repressed by glucose. however, the activity of isopenicillin n synthase wa ...	1986	3794640
suppression of free fatty acids and triglycerides in normal and hypertriglyceridemic rats by the adenosine receptor agonist phenylisopropyladenosine.	activation of adenosine receptors leads to the inhibition of lipolysis in the fat cells of several species including humans. we have examined the effects of the adenosine analog phenylisopropyladenosine (pia) on lipolysis in vivo in intact rats. pia suppresses markedly serum-free fatty acid (ffa) concentrations when injected s.c. (-)-pia is more potent than (+)-pia in suppressing ffa concentrations; also, this effect of (-)-pia is blocked by the adenosine receptor antagonist 8-(p-sulfophenyl)the ...	1986	3795036
inhibition of the platelet activating factor (paf)-induced in vivo responses in rats by trans-2,5-(3,4,5-trimethoxyphenyl) tetrahydrofuran (l-652,731), a paf receptor antagonist.	trans-2,5-bis-(3,4,5-trimethoxyphenyl)tetrahydrofuran (l652, 731), a potent and specific receptor antagonist of platelet activating factor (paf) (hwang et al., j. biol. chem. 260: 15639-15645, 1985), potently inhibits several paf-induced in vivo responses in rats. intravenously and p.o. administered l-652,731 gave a dose-response inhibition of paf-induced lysosomal hydrolase secretion and extravasation with ed50 values of 1 and 3 mg/kg, respectively. inhibitions of 87% were achieved with 50 mg/k ...	1986	3795043
[role of the diabetogenic factor in the activation of the blood coagulation system in experimental alloxan diabetes].		1986	3797076
response of dogs to challenge with ancylostoma ceylanicum during the tenure of a primary hookworm infection.	a model of human hookworm infection has been developed which shows that dogs currently infected with small numbers of hookworms are considerably resistant to a challenge infection with a large inoculum of infective larvae. two groups of dogs were infected with 500 larvae and four weeks after infection one group together with a previously uninfected control group were infected with 5,000 larvae and followed for six weeks. when compared with the secondary infection control dogs, faecal egg excreti ...	1986	3798534
rabbit endocervical epithelium: morphometric analysis of secretory cell populations.	in this report we quantitated ultrastructural changes in two cytologically distinct secretory cell populations from the rabbit endocervix. type i and type ii cells from estrous animals differ only in the presence of one or more empty cytoplasmic vacuoles in type ii cells. comparing type ii cells from 5-day pseudopregnant (psp) rabbits with type ii cells from estrous controls, there is no increase (p greater than .05) in the average vacuole volume. when type i and type ii cells from psp animals a ...	1986	3799998
[changes in the characteristics of glucocorticoid hormone binding in human peripheral blood lymphocytes during myocardial infarct].	competitive binding was used to determine the number of binding sites to glucocorticoid hormones in peripheral blood leukocytes of normal subjects, patients with unstable angina pectoris and patients with myocardial infarction. a sharp (2-3-fold) increase in the number of binding sites was observed in patients with myocardial infarction during the first 24 hours after the onset of the disease.	1986	3801148
a contribution to indirect ophthalmotonometry in beagles.	a test of suitability of the electroimpression tonometer from fritz schwarzer gmbh, munich, for measuring intraocular pressure in dogs was performed. intraocular pressure of 63 clinically healthy english beagles was measured in both eyes using the instrument with different plunger weights. intraocular pressure and volume of corneal depression during measurement were determined as a function of the plunger weight from readings on the tonometer with the aid of friedenwald's tables (2). using fried ...	1986	3807468
inferior olive lesion induces long-term modifications of cerebellar inhibition on deiters nuclei.	the frequency of discharge of cerebellar purkinje cells and lateral vestibular nuclear cells were recorded at different intervals of time after injection of 3-acetylpyridine (3ap), which destroys the inferior olivary nucleus. during the first few days, purkinje cells showed an increase of simple spike firing, while deiters cells showed a strong depression of their discharge. recordings up to 3 months demonstrated, for both groups of cells, a recovery, whose time course is faster for deiters cell ...	1986	3808481
inferior olive lesion induces long-term modifications of cerebellar inhibition on deiters nuclei.	the frequency of discharge of cerebellar purkinje cells and lateral vestibular nuclear cells were recorded at different intervals of time after injection of 3-acetylpyridine (3ap), which destroys the inferior olivary nucleus. during the first few days, purkinje cells showed an increase of simple spike firing, while deiters cells showed a strong depression of their discharge. recordings up to 3 months demonstrated, for both groups of cells, a recovery, whose time course is faster for deiters cell ...	1986	3808481
the hypoxic ventilatory response of rats with increased blood oxygen affinity.	experimental work has established an interspecific relationship between the threshold for the hypoxic ventilatory response and the 'knee' of the hb-o2 dissociation curve. however, whether this relationship exists intraspecifically remains unclear. to examine the problem further rats were treated with sodium cyanate (naocn) to lower p50 and their hypoxic ventilatory response was measured. naocn treated rats had a lower pao2 and higher hct than control rats. there was no difference between the con ...	1986	3809758
the hypoxic ventilatory response of rats with increased blood oxygen affinity.	experimental work has established an interspecific relationship between the threshold for the hypoxic ventilatory response and the 'knee' of the hb-o2 dissociation curve. however, whether this relationship exists intraspecifically remains unclear. to examine the problem further rats were treated with sodium cyanate (naocn) to lower p50 and their hypoxic ventilatory response was measured. naocn treated rats had a lower pao2 and higher hct than control rats. there was no difference between the con ...	1986	3809758
efficacy of single-agent therapy with azlocillin, ticarcillin, and amikacin and beta-lactam/amikacin combinations for treatment of pseudomonas aeruginosa bacteremia in granulocytopenic rats.	the efficacy of azlocillin, ticarcillin, and amikacin as single agents and the penicillin/amikacin combinations for treatment of pseudomonas aeruginosa bacteremia during cyclophosphamide-induced severe neutropenia in a rat model were assessed. equivalent antibiotic dosing was based on the time rat serum antibiotic levels were above the minimal bactericidal concentration for the challenge organism. antibiotic therapy was administered for 62 hours after bacterial challenge. antimicrobial efficacy ...	1986	3636060
furosemide lowers intracranial pressure by inhibiting csf production.	furosemide administration effectively lowers intracranial pressure in newborn preterm and term rabbit pups. this effect may be due to the diuretic action of the drug, its ability to inhibit cerebrospinal fluid production or to a combination of both. to test these possibilities newborn rabbits were either injected with furosemide and left unmolested for 6 hours, or anaesthetized and subjected to ventriculocisternal perfusions. during the 6 hour postnatal period the decrease in body weight was 8 t ...	1986	3811613
dose-titration of fenbendazole in the treatment of poultry nematodiasis.	broiler chickens were fed ad libitum diets containing fenbendazole at various concentrations and durations for the removal of adult ascaridia galli, heterakis gallinarum, and capillaria obsignata. all regimens were 100% effective against a. galli. fenbendazole at doses of 80 ppm for 3 days and 48 or 30 ppm for 5 days was 100% effective against h. gallinarum. c. obsignata was not removed completely by any regimen, but greater than or equal to 95% effectiveness was seen in birds given fenbendazole ...	1986	3814008
anticholinesterase properties of methamidophos and acephate in insects and mammals.		1987	3814841
use of nude mouse xenografts as preclinical drug screens: in vivo activity of established chemotherapeutic agents against melanoma and ovarian carcinoma xenografts.	to evaluate the utility of nude mouse xenografts as preclinical drug screens, the activity of ten established chemotherapeutic agents was evaluated against seven melanoma and three ovarian carcinoma xenografts. xenografts were established using primary explants from patients who had not received chemotherapy and serially passaged as sc tumors in nude mice. in vivo drug activities for dactinomycin, carmustine, vinblastine, melphalan, amsacrine, cisplatin, bleomycin, mitomycin, doxorubicin, and et ...	1987	3815395
influence of low-dose beta-interferon on natural killer cell activity in breast cancer patients subjected to chemotherapy.	the present study was designed to test whether immunomodulating doses of human beta-interferon would affect the natural cell-mediated cytotoxic function in untreated breast cancer patients or in those subjected to antitumor therapy. analyses were performed on 11 breast cancer patients, 3 at stage 1 and 8 at stage 2, the latter being subjected to cyclophosphamide, methotrexate, 5-fluorouracil (cmf) adjuvant chemotherapy. five patients treated with cmf and 3 patients not subjected to adjuvant chem ...	1987	3815421
influence of low-dose beta-interferon on natural killer cell activity in breast cancer patients subjected to chemotherapy.	the present study was designed to test whether immunomodulating doses of human beta-interferon would affect the natural cell-mediated cytotoxic function in untreated breast cancer patients or in those subjected to antitumor therapy. analyses were performed on 11 breast cancer patients, 3 at stage 1 and 8 at stage 2, the latter being subjected to cyclophosphamide, methotrexate, 5-fluorouracil (cmf) adjuvant chemotherapy. five patients treated with cmf and 3 patients not subjected to adjuvant chem ...	1987	3815421
incidence of shigellosis and multi-drug resistant shigellae: a 10 year study.	between 1976 and 1985 a total of 12,709 faecal samples were cultured. from these samples 760 strains (6.0%) of shigellae were isolated. shigella flexneri was the commonest isolate (55%), followed by s. dysenteriae (29.7%). a total of 15.7% of children under 5 years of age were positive for shigellae. the rate of isolation was lower during the winter months than in the summer months. only 25 strains (3.3%) were sensitive to all five antibiotics tested. a total of 617 (81.2%) were resistant to thr ...	1987	3820357
reaction to injectable collagen: results in animal models and clinical use.	since its commercial release, zyderm collagen implant has been used to treat more than 200,000 subjects in the united states for soft-tissue contour defects and more than 250,000 patients internationally (including the united states). approximately 3 percent of subjects' skin tested with zyderm collagen experience localized hypersensitivity reactions to collagen, whereas approximately 1 percent of treated patients demonstrate symptoms of hypersensitivity at treatment sites. of the latter treatme ...	1987	3823250
circulating forms of human foetal somatomedin.	two forms of somatomedin, which crossreact in a radioreceptor-assay using foetal brain membranes as matrix, have been partially purified from the serum of human foetuses aged 16-28 weeks of gestation. the purification scheme consisted of acid precipitation, sephadex g-50 chromatography, affinity chromatography and cation exchange fast protein liquid chromatography. the two peaks of activity had apparent molecular weights of approximately 7000 and different isoelectric points. the elution positio ...	1987	3811771
treatment of cerebrospinal fluid with formalin from patients infected with human immunodeficiency virus before diagnostic microscopy.		1987	3643930
nonproductive, cell-associated virus exists before the appearance of antiviral antibodies in experimental measles encephalitis.	the ability of measles virus to induce a cell-associated state of infection in the central nervous system (cns) plays an important role in the pathogenesis of subacute sclerosing panencephalitis (sspe), a fatal disease of children and young adults. the mechanism by which such an infection state is established is unknown, but several hypotheses have been proposed. one of these suggests that antiviral antibodies induce the nonproductive infection state by a process termed antigenic modulation. res ...	1987	3811233
the alpha 2-adrenoceptor agonists clonidine, tl99 and dpi enhance vasoconstrictor responses to sympathetic nerve stimulation and noradrenaline in the rat tail artery preparation.	1. clonidine (1-30 nmol/l) enhanced the vasoconstrictor responses to sympathetic nerve stimulation in isolated perfused artery preparations from the rat tail. higher concentrations of clonidine produced vasoconstriction in most artery preparations and reduced the response to sympathetic nerve stimulation. 2. the enhancing effect of clonidine was not affected by prazosin but was blocked by idazoxan, indicating that it was due to activation of alpha 2-adrenoceptors. 3. the alpha 2-adrenoceptor ago ...	1987	2896082
monoclonal antibodies specific for shigella flexneri lipopolysaccharides: clones binding to type iv, v, and vi antigens, group 3,4 antigen, and an epitope common to all shigella flexneri and shigella dysenteriae type 1 stains.	monoclonal antibodies reactive with shigella flexneri o antigens were generated in both mouse and rat systems. antibody-producing hybridomas were screened in an enzyme-linked immunosorbent assay using chemically defined lipopolysaccharides as antigens, and the epitope specificities were determined with a panel of lipopolysaccharides and synthetic o-antigen-specific glycoconjugates as antigens. to verify the specificity seen in the enzyme-linked immunosorbent assay, the antibodies were used in ag ...	1987	2437036
[genetic characteristics and trends in the spread of a new shigella flexneri subserovar with the antigenic formula iv:7,8].	as the result of experiments with the conjugation of s. flexneri strains 4 belonging to an unusual subserovar (iv: 7,8) with escherichia coli donor strains k12 hfr c and hfr h, as well as experiments with converting phages iv and 7,8, this new subserovar of s. flexneri 4, similarly to other s. flexneri subserovars, was proved to be the y-variant of shigellae rendered lysogenic by the two above phages. the experiments also revealed that 97.9% of all s. flexneri strains 4 (iv: 7,8) under study pos ...	1987	2437740
the novel carbohydrate epitope l3 is shared by some neural cell adhesion molecules.	the monoclonal l3 antibody reacts with an n-glycosidically linked carbohydrate structure on at least nine glycoproteins of adult mouse brain. three out of the l3 epitope-carrying glycoproteins could be identified as the neural cell adhesion molecules l1 and myelin-associated glycoprotein, and the novel adhesion molecule on glia. expression of the l3 carbohydrate epitope is regulated independently of the protein backbone of these three glycoproteins. based on the observation that out of three fun ...	1987	2438289
characterization of five shigella flexneri variant y-specific monoclonal antibodies using defined saccharides and glycoconjugate antigens.	the structural domains of the shigella flexneri variant y o-antigen epitopes 3,4 have defied definition, despite knowledge of the structure of the linear polysaccharide chain of the lps molecule. the dual epitope designation of group antigen 3,4 is based on absorption data using polyvalent rabbit antisera. five monoclonal antibodies specific for the y antigen, generated after immunization of balb/c mice or lou/c rats, were selected on the basis of elisa by using well-characterized s. flexneri y ...	1987	2438343
structure and biology of shigella flexneri o antigens.		1987	2438412
increase of enkephalin and decrease of substance p immunoreactivity in the dorsal and ventral striatum of the rat after midbrain 6-hydroxydopamine lesions.	unilateral 6-hydroxydopamine lesions of the mesostriatal dopaminergic system in the rat resulted in a decrease of substance p-immunoreactivity in the ventral striatum, and in a heterogeneously distributed increase of enkephalin-immunoreactivity in the dorsal and ventral striatum and the globus pallidus. the respective decrease and increase are caused by a lower or higher staining intensity of the peptidergic fibers, whereas no changes were found in the cell bodies.	1987	2440523
[enhancement of the therapeutic efficacy of blood substitutes in burn shock].		1987	2440759
a new set of monoclonal antibodies to human mhc class ii alpha chains demonstrates that most alpha epitopes are inaccessible on the living cell surface.	when mice were immunized with a mixture of human mhc class ii alpha and beta glycoprotein chains, the predominant antibody response was anti-alpha, and from a subsequent fusion experiment over 60 hybridomas showing anti-alpha activity were generated, compared with 11 anti-beta secretors. these findings contrast with the relative paucity of anti-alpha monoclonals described previously. use of a miniaturized western blot screening protocol was a critical factor in the present study since the anti-a ...	1987	2440796
a new set of monoclonal antibodies to human mhc class ii alpha chains demonstrates that most alpha epitopes are inaccessible on the living cell surface.	when mice were immunized with a mixture of human mhc class ii alpha and beta glycoprotein chains, the predominant antibody response was anti-alpha, and from a subsequent fusion experiment over 60 hybridomas showing anti-alpha activity were generated, compared with 11 anti-beta secretors. these findings contrast with the relative paucity of anti-alpha monoclonals described previously. use of a miniaturized western blot screening protocol was a critical factor in the present study since the anti-a ...	1987	2440796
[determination of serum antibody avidity compared to infectious o-antigenemia in paratyphoid b patients].	the avidity of serum antibodies depending on infectious o-antigenemia in patients with paratyphoid b has been studied in the course of the disease. the study has revealed a faintly pronounced inverse correlation between the degree of avidity of serum antibodies and the level of infectious antigenemia. the avidity of serum antibodies in paratyphoid b has been found to increase in the course of the disease. a significant dependence of the severity of the disease on the avidity of serum antibodies ...	1987	2441547
[nonspecific and antigen-specific adhesiveness of circulating lymphocytes in dysentery patients undergoing combined therapy using thymalin and indomethacin].	nonspecific adhesiveness of lymphocytes to polyacrylonitrile fiber and the antigen-specific adhesiveness of t-lymphocytes to shigella flexneri pellicular immunosorbent have been studied in dysentery patients. short courses of treatment with indomethacin, but not with thymalin (the preparation of thymic hormones), used for immunomodulation, have accelerated the normalization of nonspecific lymphocyte adhesiveness during regression of the disease, while indomethacin, in contrast to thymalin, inhib ...	1987	2441548
discrimination of hepatocyte-stimulating activity from human recombinant tumor necrosis factor alpha.	the involvement of tumor necrosis factor alpha (tnf alpha) in the regulation of acute-phase protein synthesis is currently under discussion. in this study the effect of human recombinant tnf alpha on the regulation of the 4 acute-phase proteins alpha 2-macroglobulin, albumin, alpha 1-proteinase inhibitor and alpha 1-acute-phase globulin was investigated in rat hepatocyte primary cultures. no changes in synthesis of any of the 4 proteins were observed. however, an acute-phase response similar to ...	1987	2441999
influence of neuropeptides on airway smooth muscle.	the first bronchoactive (and vasoactive) peptide to be discovered in the lung was isolated and characterized in 1970 from an embryologically related organ, the small intestine. since then, more than 20 additional peptides have been described in lung tissue and their biologic activities investigated. many of these are neuropeptides acting as neurotransmitters or neuromodulators to influence airway, pulmonary vascular, and other functions. more neuropeptides are known to exist in the brain and per ...	1987	2446543
acetylcholine receptor-specific human t-lymphocyte lines.		1987	2446551
definition of myasthenogenic sites of the human acetylcholine receptor using synthetic peptides.	experimental autoimmune myasthenia gravis (eamg) and antibodies that modulate achrs from cultured human muscle are induced by a disulfide-looped peptide comprising the human acetylcholine receptor (achr) alpha-subunit residues 125-147 (h alpha 125-147). to delineate the essential antigenic requirements for induction of eamg by this peptide, a series of peptides was synthesized: (a) a nonlooped analog (cys 128 replaced by ser) stimulated modulating autoantibodies, induced eamg, and bound antibodi ...	1987	2446556
distributions of certain neuropeptides in the primate thalamus.	the distributions of fibers and terminals immunoreactive for somatostatin (srif), neuropeptide y (npy), substance p (sp) and cholecystokinin octapeptide (cck), were studied in the diencephalon of cynomolgus monkeys. immunoreactivity for all 4 peptides is found in extrinsic afferent fibers innervating the dorsal thalamus, ventral thalamus and epithalamus. the distributions of such fibers are more extensive than previously described and include many relay nuclei in their zones of terminations. sp ...	1987	2446708
bw a575c, a chemically novel agent with angiotensin converting enzyme inhibitor and beta-adrenoceptor-blocking properties.	bw a575c (n-(1-(s)-carboxy-5-[4(3-isopropylamino-2-(r, s)-hydroxypropoxy)indole-2- carboxamido]pentyl)-(r, s)-alanyl-(s)-proline) is a chemically novel agent which exhibits in a single molecule both angiotensin converting enzyme (ace) inhibition and beta-adrenoceptor-blocking properties. bw a575c produced a competitive blockade of heart rate responses to isoprenaline in a guinea-pig right atrial preparation (pkb 7.18 +/- 0.05, cf. pindolol 8.9 +/- 0.7). bw a575c inhibited a partially purified pr ...	1987	2882805
glutathione metabolizing enzyme activities in human thyroid.	glutathione peroxidases, glutathione transferase, glutathione reductase and gamma-glutamyl transpeptidase activities were analyzed in human thyroid tissues obtained from 17 patients undergoing resectional surgery because of a malignancy. it was deduced, from measurements of glutathione peroxidase activity with both h202 and cumene hydroperoxide, that thyroid contains only the selenium enzyme. the absence of selenium independent glutathione peroxidase activity in thyroid was confirmed with gel fi ...	1987	2883074
a competitive receptor binding radioassay for beta-1 and beta-2 adrenergic agents.	a rapid and sensitive competitive receptor binding assay for beta-1 and beta-2 adrenergic binding for adrenergic agents has been developed. the steps that are critical for the success of the assay are given in detail so that the assay can be set up in any routine laboratory with relative ease. the rationale behind the use of specific reagents is discussed. the assay requires microgram quantities of test compound, a radiolabeled specific beta adrenergic antagonist [3h]dihydroalprenolol (dha), and ...	1987	2883662
effects of light- and dark-rearing on the postnatal development of gaba receptor sites in the chick optic lobe.	to investigate the ability of gaba receptor sites to undergo environmental-dependent plastic changes, the postnatal developmental pattern of gaba receptors was studied under different levels of light stimulation, i.e. normal-, light- and dark-rearing. at hatching the specific binding of [3h]gaba was 1.74 +/- 0.36 pmol/optic lobe. in normally reared chicks the number of gaba binding sites showed a transient increase with the highest value at the 6th day (7.0 +/- 1.32 pmol/optic lobe). this value ...	1987	2845719
beta-blockade reverses regional dysfunction in ischemic myocardium.	to determine the protective effect of oxprenolol-induced beta-blockade on the compromised myocardium (critical constriction of the left anterior descending coronary artery) against the adverse effect of high concentrations of halothane, halothane dose-response curves were obtained in six dogs in each of three phases: preconstriction (control), critical constriction, and critical constriction with the addition of 0.3 mg/kg intravenous oxprenolol. the extent of depression of ventricular function w ...	1987	2886076
composite effects of actinonin when inhibiting enkephalin-degrading enzymes.	actinonin which has been found to be an inhibitor of aminopeptidase m (ec 3.4.11.2) also inhibited enkephalinase a (ec 3.4.24.11) and enkephalin aminopeptidase which were partially purified from the corpus striatum membrane of guinea-pig brain. the ic50 values were 5.6 microm for enkephalinase a and 0.39 microm for enkephalin aminopeptidase. actinonin also inhibited with an ic50 value of 1.1 microm dipeptidyl aminopeptidase tested on whole brain homogenate of rats in the presence of thiorphan an ...	1987	2886348
azodisalicylate (azodisal sodium) causes intestinal secretion. comparative study of the effect of azodisalicylate, sulfasalazine, 5-aminosalicylic acid and sulfapyridine on the water and electrolyte transfer and the morphology of the rat ileum and colon in vivo.	azodisalicylate (ads) is one of the possible successors of sulfasalazine in the treatment of ulcerative colitis. the following results were obtained when comparing the influence of ads on net water and electrolyte transfer in tied-off loops of the rat ileum and colon in vivo with sulfsalazine, 5-aminosalicylic acid, and sulfapyridine. (1) sulfasalazine, 5-aminosalicylic acid and sulfapyridine had no effect on water and electrolyte transfer of the healthy intestinal mucosa in concentrations up to ...	1987	2887473
activation of a common effector system by different brain neurotransmitter receptors in xenopus oocytes.	xenopus oocytes possess 'native' muscarinic receptors, which give rise to oscillatory chloride currents; similar responses are elicited by activation of foreign receptors to serotonin, glutamate and noradrenaline, expressed in oocytes after injection of messenger rna from rat brain. when low concentrations of two agonists are applied together, the combined response is greater than would be expected from the sum of the responses to each agonist applied alone. potentiation of acetylcholine by sero ...	1987	2888117
enhancement of contractile responses to partial alpha-adrenoceptor agonists during warming in rat aorta.	we examined the effects of warming on the contractile responses to full and partial alpha-adrenoceptor agonists in rat aorta. the contractions elicited by norepinephrine and methoxamine were not affected during warming (40 degrees c, 42 degrees c), whereas those induced by clonidine and st 587 were significantly enhanced. kcl-induced contractions of rat aorta were not affected by warming. the dissociation constants of clonidine and st 587 at 40 degrees c were not different from those at 37 degre ...	1987	2895427
plasma levels of gastrin, somatostatin, and cholecystokinin immunoreactivity during pregnancy and lactation in dogs.	plasma levels of gastrinlike, somatostatinlike, and cholecystokininlike immunoreactivity were determined by radioimmunoassay in peripheral blood from 11 beagles during pregnancy and in response to suckling during lactation. cholecystokinin was determined in pooled and in individual plasma samples after separation of cholecystokinin and gastrin by high-performance liquid chromatography. gastrin and somatostatin levels were significantly increased during the 8-wk-long dog pregnancy; peak values we ...	1987	2880780
media for isolating shigella.		1987	2881104
"sub-threshold neoplastic states" created in transgenic mice.	several possible scenarios are already suggested by the first series of studies in which a high risk situation with respect to malignant diseases was artificially created by introducing oncogenes in the mouse germ line. only in exceptional cases was the expression of the transgene followed in an obligatory way by cancerous proliferation. the usual situation appears now to be that somatic cells expressing the transgene have only a higher probability than normal cells of undergoing a subsequent tr ...	1987	2835726
"sub-threshold neoplastic states" created in transgenic mice.	several possible scenarios are already suggested by the first series of studies in which a high risk situation with respect to malignant diseases was artificially created by introducing oncogenes in the mouse germ line. only in exceptional cases was the expression of the transgene followed in an obligatory way by cancerous proliferation. the usual situation appears now to be that somatic cells expressing the transgene have only a higher probability than normal cells of undergoing a subsequent tr ...	1987	2835726
cloned mouse lymphocytes permit analysis of somatic mutations that occur in vivo.	as part of our mouse model of somatic mutation, we have begun to characterize spontaneously occurring hypoxanthine phosphoribosyltransferase (hprt) -deficient mouse lymphocytes. lymphocytes were cloned by in vitro exposure of spleen cells from male c57b1/6 mice to the mitogen concanavalin a, conditioned medium containing lymphocyte growth factors, and thioguanine (tg), in a limiting dilution assay. the 17 tg-resistant clones recovered were all highly deficient in hprt activity and were found by ...	1987	2842875
localization of plasmid loci necessary for the entry of shigella flexneri into hela cells, and characterization of one locus encoding four immunogenic polypeptides.	we have previously cloned a 44 kb fragment from the virulence plasmid of shigella flexneri serotype 5 strain m90t which is capable of restoring invasiveness to an avirulent, plasmidless mutant. this report presents a genetic and physical analysis of tn5 mutations in recombinant clone phs4108. tn5 mutagenesis allowed identification of at least five regions implicated in the entry phenotype. these regions were located on a 20 kb portion of phs4108. expression of the insertion mutants was studied b ...	1987	2846749
metabolic events mediating early killing of host cells infected by shigella flexneri.	j774, a continuous macrophage cell-line, was infected by m90t, an invasive isolate of shigella flexneri serotype 5 and bs176, its non invasive derivative--which does not harbor the 220 kbase virulence plasmid pwr100. killing of host cells by intracellular m90t, commenced one hour after infection and was completed by 4 hours. intracellular bs176 did not kill cells during the same period. cell protein biosynthesis was totally inhibited by both strains within 2 hours of infection thus indicating th ...	1987	2848171
identification of recessive mutations at human cancer loci.		1987	2820528
in vivo measurements of tissue ph of the heart, brain, liver, spleen, and skeletal muscle following therapy of severe metabolic uraemic acidosis with infusion of bases: an experimental study on the limitation of the mellemgaard-astrup equation.	the intracellular tissue ph of the heart, brain, liver, spleen, and skeletal muscle of nephrectomized rats was determined in vivo from the distribution of 14c-labelled dmo (5,5-dimethyl-2-4-oxazolidinedione) following acute variation of arterial plasma ph (phe) by co2 inhalation or sodium bicarbonate administration in the range of phe 6.9-7.7, or following correction of severe metabolic uraemic acidosis with sodium bicarbonate or tris (hydroxymethyl) aminomethane (tham) infusion according to the ...	1987	2820724
atp synthesis by the (na+ + k+)-atpase in the absence of an ionic gradient. effects of organic solvent.	a 200-fold decrease in the pi concentration required for half-maximal phosphorylation of the (na+ + k+)-atpase is observed when 40% (v/v) dimethyl sulfoxide is added to the assay medium. the phosphoenzyme formed in the presence of dimethyl sulfoxide is able to transfer its phosphate to to form atp when concentrations of organic solvent and of na+, and is inhibited by ouabain.	1987	2820794
atp synthesis by the (na+ + k+)-atpase in the absence of an ionic gradient. effects of organic solvent.	a 200-fold decrease in the pi concentration required for half-maximal phosphorylation of the (na+ + k+)-atpase is observed when 40% (v/v) dimethyl sulfoxide is added to the assay medium. the phosphoenzyme formed in the presence of dimethyl sulfoxide is able to transfer its phosphate to to form atp when concentrations of organic solvent and of na+, and is inhibited by ouabain.	1987	2820794
frequency of is1-mediated molecular events in different members of the family enterobacteriaceae.	the numbers of chromosomal copies of the insertion sequence is1 in strains of salmonella typhimurium (0 to 8 copies), shigella sonnei (56 copies), and shigella flexneri (41 copies) isolated in mexico city, mexico, were similar to those reported for these genera isolated in other countries. of the 11 shigella strains studied, all carried several small plasmids; however, in only one of these strains did a small plasmid contain is1, is1 recombination, cointegrate formation mediated by is1 or by the ...	1987	2822657
induction of phr gene expression by irradiation of ultraviolet light in escherichia coli.	to measure the degree of phr gene induction by dna-damaging agents, the promoter region was fused to the coding region of the lacz gene in plasmid pmc1403. the new plasmids were introduced into escherichia coli cells having different repair capabilities. more efficient induction of phr gene expression was detected in a uvra- strain as compared with the wild-type strain. in addition, obvious induction was detected in uvra- cells treated by 4-nitroquinoline 1-oxide and mitomycin c. nalidixic acid, ...	1987	2823069
induction of phr gene expression by irradiation of ultraviolet light in escherichia coli.	to measure the degree of phr gene induction by dna-damaging agents, the promoter region was fused to the coding region of the lacz gene in plasmid pmc1403. the new plasmids were introduced into escherichia coli cells having different repair capabilities. more efficient induction of phr gene expression was detected in a uvra- strain as compared with the wild-type strain. in addition, obvious induction was detected in uvra- cells treated by 4-nitroquinoline 1-oxide and mitomycin c. nalidixic acid, ...	1987	2823069
morphological differentiation of cultured astrocytes is blocked by cadmium or cobalt.	the morphological transformation of astrocytes in primary culture from flat to process bearing is induced by cyclic adenosine monophosphate (camp). calcium currents in cultured astrocytes are also enhanced by camp. this correlation suggests the hypothesis that camp-induced calcium entry causes the morphological differentiation. the experiments reported here show that cadmium or cobalt, which block astrocyte calcium channels, also block the camp-induced morphological differentiation. these result ...	1987	2823972
isolation and characterization of is elements repeated in the bacterial chromosome.	shigella sonnei contains repetitive sequences, including an insertion element is1, which can be isolated as double-stranded dna fragments by dna denaturation and renaturation and by treatment with s1 nuclease. in this paper, we describe a method of cloning the is1 fragments prepared by the s1 nuclease digestion technique into phage m13mp8 rfi dna. several clones contained is1, usually with a few additional bases. we isolated and characterized five other repetitive sequences using this method. on ...	1987	2824781
effects of palindrome size and sequence on genetic stability in the bacteriophage phi x174 genome.	by inserting palindromes of varying length and sequence into a non-essential region of the bacteriophage phi x174 genome we have investigated the effect of palindrome size and sequence on their genetic stability. multimers of increasing size of the ecori linker ccgaattcgg (e), the bamhi linker ccggatccgg (b) or mixtures of both (e, b) were inserted into the pvuii site of a previously constructed bacteriophage strain phi x174 j-f ins6. the largest inserts that could be maintained in the genome wi ...	1987	2824789
dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites.	selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ...	1987	2825926
dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites.	selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ...	1987	2825926
genetic and physical characterization of trimethoprim resistance plasmids from shigella sonnei and shigella flexneri.	analysis of six shigella flexneri and four s. sonnei isolates with trimethoprim (tp) resistance from clinical cases in ontario has shown that, in all isolates, the tp resistance is mediated by gene(s) on conjugative, multiple antibiotic-resistance plasmids. the physical and genetic characterization of these plasmids revealed that there are three different tp resistance plasmids. one group, composed of all six s. flexneri plasmids, consists of plasmids which are about 70 megadaltons (mda) and inh ...	1987	2825949
murine cytomegalovirus-pseudomonas synergistic infections: comparison of virulent and attenuated virus.	the synergistic interactions between pseudomonas aeruginosa and virulent or attenuated murine cytomegalovirus (mcmv) were compared in vivo. virulent mcmv challenge at a dose of 5 x 10(5) pfu/mouse intraperitoneally, followed by intranasal superinfection with 5 x 10(6) cfu/mouse of pseudomonas aeruginosa after 48 h resulted in greater than 80% mortality, apparently owing to a failure of pulmonary clearance mechanisms. single infections, or the use of attenuated mcmv in synergistic infections, did ...	1987	2825950
limb specific connections of the cat magnocellular red nucleus.	afferent and efferent connections of the limb specific divisions of the cat magnocellular red nucleus (rnm) were traced using the bidirectional transport of wheatgerm agglutinin-horseradish peroxidase complex (wga-hrp). injection sites within forelimb or hindlimb rnm regions were identified by microelectrode recording and confirmed by the position of labeled rubrospinal terminals. additional injections into structures that project to, or receive input from, rnm confirmed the somatotopic organiza ...	1987	2826551
metabolic studies of a sustained galactose overload in rat.	in order to obtain an experimental model for studying inherited disorders of galactose metabolism in man, we administered to adult rats a diet supplemented with 50% of galactose during 38 days. the results showed clinical symptoms such as polyuria, polydipsia, growth retardation and bilateral cataract. the main biochemical features were an increase in the galactokinase and uridyltransferase activities in liver, an ubiquitous accumulation in tissues and a considerable urinary elimination of galac ...	1987	2827561
synthesis and biological activity of n-methylated analogues of neurokinin a.	limited conformational constraints have been introduced in the sequence of the c-terminal heptapeptide nka(4-10) by n-methylation of individual peptide bonds and the biological activities of the peptides thus obtained were evaluated on three in vitro preparations sensitive and quite selective for each of the naturally-occurring neurokinins, in order to assess the effect of n-methylation on receptor selectivity. the analogue methylated in position 7, namely [meval7] nka(4-10) shows some selectivi ...	1987	2829053
identification of tonin in brain and exocrine tissues and in the cell-free translation products encoded by the mrna of these tissues.	tissue-specific expression of the esteropeptidase tonin [ec 3.4.99.-] was investigated in rat brain, submandibular gland, pancreas and kidney. specific polyclonal and monoclonal antibodies to purified rat tonin from the submandibular gland have been developed and characterized and have been purified via a tonin-agarose affinity column. immunoreactive tonin was measured by a recently developed tonin direct radioimmunoassay using a rabbit tonin antiserum. resulting tonin levels were found to be 10 ...	1987	2829829
cytomegalovirus infection in burn patients.	we studied the seroepidemiology of cytomegalovirus (cmv) infection in 59 patients with thermal injuries. among the 31 patients who were initially cmv seronegative, seven (22.5%) seroconverted. patients who seroconverted had longer hospital stays (mean, 27 +/- 23.9 days v 14.9 +/- 8.8 days in seronegative patients [p = 0.03]) and they received more blood products (mean, 10.7 +/- 17.9 units v 1.8 +/- 2.8 units [p = 0.02]) than patients who remained seronegative. among 18 patients who were initiall ...	1987	2830289
structural homology of human complement component c8 gamma and plasma protein hc: identity of the cysteine bond pattern.	anti-c8 alpha-gamma specific antibodies were used to isolate cdna clones from a human liver expression library. antibodies affinity-purified on the expressed hybrid protein of one clone bound exclusively to the gamma-chain of reduced c8 alpha-gamma. this clone, as well as a second full length cdna clone obtained by hybridization screening, were sequenced and the complete primary structure for c8 gamma was established. cyanogen bromide cleavage of c8 alpha-gamma released a 12 kda carboxy-terminal ...	1987	2447883
antiviral chemotherapy and prophylaxis of viral respiratory disease.	respiratory viruses continue to be major causes of morbidity and mortality. currently available chemotherapy is limited to oral amantadine for uncomplicated influenza a and aerosolized ribavirin for respiratory syncytial virus (rsv) infections. amantadine is also efficacious for chemoprophylaxis of influenza a virus infections. rimantadine has similar clinical efficacy and is better tolerated than amantadine. aerosolized ribavirin may be useful in the treatment of serious respiratory illness cau ...	1987	2446820
theophylline's inhibition of antigen-induced contraction of human parenchymal strips is independent of adenosine antagonism.	the effects of theophylline, and adenosine antagonist, and enprofylline, reported to lack adenosine antagonism, were studied using antigen-induced contraction of sensitized human lung parenchymal strips. cumulative addition of adenosine usually resulted in a small concentration-dependent contraction, which was antagonized by theophylline, 100 microm. antigen-induced contraction was reduced by pretreatment with adenosine, 10 microm, but not by adenosine addition after antigen challenge. dipyridam ...	1987	2446888
flanking and internal regions of chromosomal genes mediating aerobactin iron uptake systems in enteroinvasive escherichia coli and shigella flexneri.	we have investigated the presence of the aerobactin system and the location of the aerobactin genes in enteroinvasive strains of escherichia coli. also, we cloned the aerobactin region and its flanking sequences from the chromosome of a strain of shigella flexneri and compared the molecular organization of the aerobactin genes in the two genera. of the 11 enteroinvasive e. coli strains studied, 5 possessed the aerobactin genes, which were located on the chromosome in each case. these strains pro ...	1987	2832521
cerebrovascular effects of isoflurane in man. intracranial pressure and middle cerebral artery flow velocity.	twelve hydrocephalic patients undergoing hydrodynamic studies and shunt procedures during general anaesthesia were subjected to short term observations of the cerebrovascular effects of isoflurane. intraventricular pressure (ivp), arterial pressure (ap); cerebral perfusion pressure (cpp = ap-ivp), end-tidal pco2, cerebral function monitor (cfm) activity and middle cerebral artery (mca) flow velocity were measured. introduction of 1% isoflurane for a 10-min period did not cause significant change ...	1987	3676050
human monoclonal cryoimmunoglobulins. ii. comparative studies on the carbohydrate contents and compositions of cryoglobulins and myeloma proteins.	the carbohydrate contents and compositions of cryoglobulins and myeloma proteins were comparatively investigated by gas-liquid chromatographic and colorimetric analyses. human monoclonal cryoglobulins, jir (igg3 kappa), wat (igg3 lambda), fji (igm kappa), and human myeloma proteins, har (iggl kappa) and kob (iga kappa) were used for the present experiments. the results obtained were that the carbohydrate contents of jir and wat were 2.7 and 4.4% in weight basis; the n-acetyl glucosamine of wat w ...	1987	3685711
distribution of immunoreactive inhibin like material in different regions of human prostate.	distribution of immunoreactive inhibin material (ir-ilm) in different regions of human prostate was studied by using specific radio-immunoassay. mean ir-ilm concentrations in the central, peripheral and periurethral zones were 27.3 +/- 9.3, 2.4 +/- 0.7 and 3.0 +/- 1.5 micrograms/mg protein respectively (p central vs periurethral and peripheral less than 0.05).	1987	3688474
effects of changed lipid composition on responses of liposomes to various odorants: possible mechanism of odor discrimination.	in a previous paper [nomura, t., & kurihara, k. (1987) biochemistry (preceding paper in this issue)], we showed that azolectin liposomes are depolarized by various odorants and there is a good correlation between the responses in the liposomes and the frog or porcine olfactory responses. in this study, we examined effects of changed lipid composition on responses of liposomes to various odorants. the membrane potential changes in response to odorants were monitored with the fluorescent dye 3,3'- ...	1987	3689768
effects of cold stress on brain regional calcium content in rats and mice.	the calcium levels of striatum, cerebellum, cortex, medulla and diencephalon from rat brain were significantly reduced after 30 minutes of cold exposure at 8 degrees c even though body temperatures were no different from pre-cold exposure values. calcium content remained significantly depressed in striatum, cerebellum and diencephalon after 2 hours of cold exposure, at which time body temperatures were significantly depressed. a similar effect of short-term cold stress on calcium content in thes ...	1987	3690357
[clinico-anatomical and immunomicrobiological aspects of infection caused by gram-negative bacteria with lung lesions in children].		1987	3696845
further improvement of high-speed nmr flow-velocity measurement using a differential phase-encoding technique.	an nmr imaging technique applicable to high-velocity flow imaging is described. by subtraction of two phase images obtained by varying the flow-encoding gradient, it is possible to extract much larger ranges of velocity components. this differential phase-encoding technique is tested by experiments in a phantom as well as a human volunteer.	1987	3574062
estrogen stimulates growth of mammary tumor cells zr-75 without activation of s6 kinase and s6 phosphorylation. difference from epidermal growth factor and alpha-transforming growth-factor-induced proliferation.	growth of the human mammary tumor cell line zr-75-1 is stimulated by epidermal growth factor (egf) and alpha-type transforming growth factor (alpha tgf), as well as by estradiol (e2). the role of activation of s6 kinase and s6 phosphorylation in the egf(alpha tgf)-induced and e2-induced growth was investigated. maximal effects on growth are observed at 10 nm egf or alpha tgf. egf as well as alpha tgf treatment of serum-starved cells leads to rapid activation of s6 kinase; the activity is increas ...	1987	3494604
dexamethasone pre-treatment is antipyretic toward polyinosinic: polycytidylic acid, lipopolysaccharide and interleukin 1/endogenous pyrogen.	the effect of intravenously injected dexamethasone on the febrile response of rabbits to polyinosinic: polycytidylic acid (poly i:c), lipopolysaccharide (lps) and interleukin 1/endogenous pyrogen (il1/e.p.) was studied. dexamethasone (1 mg/kg) attenuated the febrile response to poly i:c (5 micrograms/kg) but only if administered between 0.5 to 2 h before poly i:c. if it was given after poly i:c this resulted in a potentiation of the fever. antagonism of the febrile response to poly i:c by dexame ...	1987	3495738
cerebrovascular response to intracranial hypertension.	pial arterial and venous calibre changes during intracranial hypertension were studied in 11 cats under barbiturate- and nitrous oxide-anaesthesia by using a closed cranial window technique and multichannel videoangiometry. intracranial pressure was elevated from a normal mean level of 6.4 mm hg by cisternal infusion of mock csf in steps to 20, 30, 40, 50 mm hg and finally to the level of systolic pressure. pial arteries dilated significantly, small ones more than large ones, by 42 +/- 5.6% and ...	1987	3577856