Publications

endogenous interleukin-18 improves the early antimicrobial host response in severe melioidosis.	melioidosis is caused by the soil saprophyte burkholderia pseudomallei and is endemic in southeast asia. the pathogenesis of melioidosis is still largely unknown, although gamma interferon (ifn-gamma) seems to play an obligatory role in host defense. previously, we have shown that ifn-gamma production in melioidosis is controlled in part by interleukin-18 (il-18). the aim of the present study was to determine the role of il-18 in the immune response to b. pseudomallei. for this the following inv ...	2007	17517876
identification of a lolc homologue in burkholderia pseudomallei, a novel protective antigen for melioidosis.	melioidosis is an emerging disease of humans in southeast asia and tropical australia. the bacterium causing this disease, burkholderia pseudomallei, is also considered a bioterrorism agent, and as yet there is no licensed vaccine for preventing b. pseudomallei infection. in this study, we evaluated selected proteins (lolc, potf, and oppa) of the atp-binding cassette systems of b. pseudomallei as candidate vaccine antigens. nonmembrane regions of the b. pseudomallei proteins were expressed and p ...	2007	17517877
chlorobenzoate inhibits growth and induces stress proteins in the pcb-degrading bacterium burkholderia xenovorans lb400.	aerobic bacteria, such as burkholderia xenovorans lb400, are able to degrade a wide range of polychlorobiphenyls (pcbs). generally, these bacteria are not able to transform chlorobenzoates (cbas), which accumulate during pcb degradation. in this study, the effects of cbas on the growth, the morphology and the proteome of burkholderia xenovorans lb400 were analysed. 4-cba and 2-cba were observed to inhibit the growth of strain lb400 on glucose. strain lb400 exposed to 4-cba exhibited increased nu ...	2007	17522847
[biofilm formation by strains of burkholderia cepacia complex in dependence of their phenotypic and genotypic characteristics].	biofilm formation was studied in 54 strains of burkholderia cepacia complex isolated in 7 moscow hospitals. 80% of strains (biofilm groups i and ii) had the capacity to biofilm formation and only 16.7% of strains (group iii) were not capable to biofilm formation. molecular genetic methods allowed to identify one of the epidemic markers (cbl, is hybrid sequence, burkholderia cepacia epidemic strain marker - bcesm) in 46.7, 23.3, and 33.3% of strains from group i, ii, and iii respectively. gene ce ...	2007	17523420
[immunogenicity of surface and capsular antigens of burkholderia].	study showed that five (c3, c6, c9, c10, c11) out of ten chromatographic fractions of surface and capsular antigens of b. mallei significantly stimulated cell-mediated immunity that manifested in activation of delayed hypersensivity reactions (dhs) and phagocyteability of noncapsulated avirulent strain of b. mallei with added surface and capsular antigenic complexes. other fractions did not stimulate cell-mediated immunity, furthermore, fraction c8, which contained capsular biopolymer with mass ...	2007	17523429
[adequate selection of antigens for serologic diagnostics of experimental melioidosis].	sera of burkholderia pseudomallei-infected golden hamsters, white mice, guinea pigs and white rats were studied. immunochemical analysis revealed presence of antibodies against antigens 2, 3, 6, d, and g with significant predominance of antibodies to antigens 6 and d. antigen d was detected irrespectively to strain used for experimental infection and experimental animal species. antibodies to antigen 6 were detected only when strains belonging to asian serovar, but not to australian serovar, wer ...	2007	17523479
description, etiology, and prevention of an outbreak of diffuse lamellar keratitis after lasik.	to describe an outbreak of diffuse lamellar keratitis (dlk) and provide a hypothesis about the etiology.	2007	17523510
role of solution chemistry and ion valence on the adhesion kinetics of groundwater and marine bacteria.	the role of solution chemistry on bacterial adhesion has been investigated using a radial stagnation point flow (rspf) system. this experimental system utilized an optical microscope and an image-capturing device to directly observe the deposition kinetics of a groundwater bacterium, burkholderia cepacia g4g, and a marine bacterium, halomonas pacifica g. experiments were carried out under well-controlled hydrodynamic and solution chemistry conditions, allowing for the sensitivity of bacterial ad ...	2007	17523680
characterization of biphenyl dioxygenase of pandoraea pnomenusa b-356 as a potent polychlorinated biphenyl-degrading enzyme.	biphenyl dioxygenase (bpdo) catalyzes the aerobic transformation of biphenyl and various polychlorinated biphenyls (pcbs). in three different assays, bpdo(b356) from pandoraea pnomenusa b-356 was a more potent pcb-degrading enzyme than bpdo(lb400) from burkholderia xenovorans lb400 (75% amino acid sequence identity), transforming nine congeners in the following order of preference: 2,3',4-trichloro approximately 2,3,4'-trichloro > 3,3'-dichloro > 2,4,4'-trichloro > 4,4'-dichloro approximately 2, ...	2007	17526697
monophyly of noda and nifh genes across texan and costa rican populations of cupriavidus nodule symbionts.	noda and nifh phylogenies for cupriavidus nodule bacteria from native legumes in texas and costa rica grouped all strains into a single clade nested among neotropical burkholderia strains. thus, cupriavidus symbiotic genes were not acquired independently in different regions and are derived from other betaproteobacteria rather than from alpha-rhizobial donors.	2007	17526782
characterization of two distinct phospholipase c enzymes from burkholderia pseudomallei.	burkholderia pseudomallei is a serious bacterial pathogen that can cause a lethal infection in humans known as melioidosis. in this study two of its phospholipase c (plc) enzymes (plc-1 and plc-2) were characterized. starting with a virulent strain, two single mutants were constructed, each with one plc gene inactivated, and one double mutant with both plc genes inactivated. the single plc mutants exhibited decreased extracellular plc activity in comparison to the wild-type strain, thereby demon ...	2007	17526847
blue-white selection of regulatory genes that affect the expression of dehalogenase iva of burkholderia cepacia mba4.	we have developed a method for rapid screening of genes that affected the expression of dehalogenase iva of burkholderia cepacia mba4. the promoter region of the dehalogenase gene was used to drive the expression of a beta-galactosidase gene. a plasmid containing this reporter was first electroporated into mba4, and a tn5 containing suicidal plasmid was introduced subsequently. the use of electroporation was necessary because escherichia coli mediated transconjugation was ineffective in plasmid- ...	2007	17530244
isolation, heterologous expression and characterization of an endo-polygalacturonase produced by the phytopathogen burkholderia cepacia.	endo-polygalacturonases (endopgs) belong to the glycoside hydrolase family 28 and hydrolyze the alpha-1,4 glycosidic bond present in the smooth regions of pectins. pectic substances are among the principal macromolecular components of the primary plant cell walls and are subjected to enzymatic degradation not only in the course of important physiological processes such as plant senescence and ripening, but also during infection events by plant pathogens. here we report, for the first time, the i ...	2007	17493828
melioidosis presenting as genitourinary infection in two men with diabetes.	melioidosis is an infectious disease caused by burkholderia pseudomallei. it occurs predominantly in tropical regions. the manifestations are protean which include pneumonia, visceral abscesses, septic arthritis, osteomyelitis, acute suppurative and chronic granulomatous lesions with involvement of almost all organ systems. fulminant sepsis is much more common and is associated with high mortality. hence prompt recognition and early treatment is warranted. we report unusual presentations of urin ...	2007	17495376
octanoyl-homoserine lactone is the cognate signal for burkholderia mallei bmar1-bmai1 quorum sensing.	acyl-homoserine lactones (hsls) serve as quorum-sensing signals for many proteobacteria. members of the luxi family of signal generators catalyze the production of acyl-hsls, which bind to a cognate receptor in the luxr family of transcription factors. the obligate animal pathogen burkholderia mallei produces several acyl-hsls, and the b. mallei genome has four luxr and two luxi homologs, each of which has been established as a virulence factor. to begin to delineate the relevant acyl-hsl signal ...	2007	17496085
bioformulation of burkholderia sp. mssp with a multispecies consortium for growth promotion of cajanus cajan.	the present work was undertaken to formulate an effective bioformulation using burkholderia sp. strain mssp, a known plant-growth-promoting rhizobacterium. mssp was tagged with the reporter gene of green fluorescent protein (gfp) to monitor its population in cost-effective solid carriers, including sugarcane-bagasse, sawdust, cocoa peat, rice husk, wheat bran, charcoal, and rock phosphate, and paneer-whey as liquid carrier. physical and chemical properties of different low-cost carrier materials ...	2007	17496969
insight into heterogeneity in cell-surface hydrophobicity and ability to degrade hydrocarbons among cells of two hydrocarbon-degrading bacterial populations.	the sequential bacterial adherence to hydrocarbons (bath) of successive generations of hydrophobic fractions of paenibacillus sp. r0032a and burkholderia cepacia gave rise to bacterial populations of increasing cell-surface hydrophobicity. thus, hydrophobicity of the first generation (h1) was less than that of the second generation (h2), which was less than that of the third generation (h3). beyond h3, the hydrophobic populations became less stable and tended to lyse in hexadecane after violent ...	2007	17496974
diverse bacteria isolated from root nodules of trifolium, crotalaria and mimosa grown in the subtropical regions of china.	to analyze the diversity and relationships of rhizobia in the subtropical and tropical zones of china, we characterized 67 bacterial strains isolated from root nodules of five legume species in the genera trifolium, crotalaria and mimosa . pcr-amplified 16s rdna rflp, numerical taxonomy, sds-page of whole cell proteins, sequencing of 16s rdna and dna-dna hybridization grouped the isolates into 17 lineages belonging to bradyrhizobium, mesorhizobium, rhizobium, sinorhizobium and burkholderia, as w ...	2007	17497134
the factors shaping synonymous codon usage in the genome of burkholderia mallei.	burkholderia mallei is regarded as a potential biological weapon by the centers for disease control and prevention. in this study, the main factors shaping codon usage in the genome of b. mallei atcc 23344 were firstly reported. the results showed that the primary trend in codon usage variation in the b. mallei is due to translational selection; while compositional mutation bias is relatively the weaker influence and the hydrophobicity of each protein and gene length are only the minor influence ...	2007	17498635
comparative proteomic analysis of b. cenocepacia using two-dimensional liquid separations coupled with mass spectrometry.	burkholderia cenocepacia is an important respiratory pathogen in persons with cystic fibrosis. we compared the proteomes of clinical and environmental isolates of b. cenocepacia by using a 2d liquid separation method coupled with mass spectrometry. proteome maps of four b. cenocepacia isolates were generated. in the first dimension, 5 mg of protein from each isolate was fractionated by chromatofocusing (cf) in the range of ph 4.0-7.0. in the second dimension, each cf fraction was separated by np ...	2007	17499075
melioidosis in a rural community of western province, papua new guinea.	a prospective study was conducted to determine the significance of melioidosis in the balimo district of western province, papua new guinea. during 1998, after the establishment of laboratory procedures and increasing local clinical awareness, the disease was found in 1.8% (95% ci 0.37-5.1%) of individuals presenting with fever refractory to standard treatment. the clinical incidence was 20.0 per 100,000 population (95% ci 12.2-30.9). the median age of culture-confirmed cases was 9.5 years (inte ...	2007	17499321
antimicrobial properties of two purified skin peptides from the mink frog (rana septentrionalis) against bacteria isolated from the natural habitat.	numerous peptides exhibiting antimicrobial properties have been isolated from the skins of many amphibian species. these peptides offer an innate chemical defense system against various microbial agents that exist in the amphibian's environment. amphibian skin peptides are typically tested for antimicrobial activity against microbial strains that are pathogenic to humans, but not on potential pathogenic or opportunistic bacteria that exist in the organism's habitat. two peptides, a brevinin-2-re ...	2007	17499556
isolation and characterization of a lipid-degrading bacterium and its application to lipid-containing wastewater treatment.	to construct an efficient lipid-containing wastewater treatment system, microorganisms that degrade lipids efficiently were isolated from various environmental sources. strain dw2-1 showed the highest rate of degradation of 1% (w/v) salad oil among the isolated strains. strain dw2-1 was identified as burkholderia sp. and designated burkholderia sp. dw2-1. the rate of degradation of salad oil, olive oil, sesame oil, and beef tallow by strain dw2-1 were 96.7%, 92.3%, 90.1% and 77.4%, respectively, ...	2007	17502273
burkholderia cenocepacia, b. multivorans, b. ambifaria and b. vietnamiensis isolates from cystic fibrosis patients have different profiles of exoenzyme production.	knowledge about the virulence mechanisms of species from the burkholderia cepacia complex (bcc) is still limited. the genomovar heterogeneity and production of different virulence factors are likely to contribute to the variation in the clinical outcome observed in bcc-infected cystic fibrosis (cf) patients. therefore, in this study we investigated the genetic polimorphism, the presence of genetic makers associated with virulence and transmissibility in bcc, and the profile of exoenzyme producti ...	2007	17504297
bacterial symbionts in the hepatopancreas of isopods: diversity and environmental transmission.	the midgut glands (hepatopancreas) of terrestrial isopods contain bacterial symbionts. we analysed the phylogenetic diversity of hepatopancreatic bacteria in isopod species from various suborders colonizing marine, semiterrestrial, terrestrial and freshwater habitats. hepatopancreatic bacteria were absent in the marine isopod idotea balthica (valvifera). the symbiotic bacteria present in the midgut glands of the freshwater isopod asellus aquaticus (asellota) were closely related to members of th ...	2007	17506824
evaluation of the colorimetric vitek 2 card for identification of gram-negative nonfermentative rods: comparison to 16s rrna gene sequencing.	ninety strains of a collection of well-identified clinical isolates of gram-negative nonfermentative rods collected over a period of 5 years were evaluated using the new colorimetric vitek 2 card. the vitek 2 colorimetric system identified 53 (59%) of the isolates to the species level and 9 (10%) to the genus level; 28 (31%) isolates were misidentified. an algorithm combining the colorimetric vitek 2 card and 16s rrna gene sequencing for adequate identification of gram-negative nonfermentative r ...	2007	17507509
pyrosequencing of a reca gene variable region for burkholderia cepacia complex genomovar identification.	we developed an assay to identify burkholderia cepacia complex genomovars by pyrosequencing of a variable reca gene segment. fifteen reference strains and 30 clinical isolates of b. cepacia complex were sequenced. full 77-base pair target sequences were obtained from 44 of the 45 isolates, and blast queries of the sequences correctly identified the genomovar of these 44 isolates. three burkholderia multivorans isolates were identified as b. multivorans/burkholderia ambifaria, indicating that add ...	2007	17509790
metal tolerating methylotrophic bacteria reduces nickel and cadmium toxicity and promotes plant growth of tomato (lycopersicon esculentum l).	inoculation of plants with microorganisms may reduce the toxicity of heavy metals to plants in contaminated soils. in this study, we have shown that the plant growth promoting bacteria methylobacterium oryzae strain cbmb20 and burkholderia sp. strain cbmb40 from rice reduce the toxicity of ni and cd in tomato and promote plant growth under gnotobiotic and pot culture experiments. the bacterial strains bound considerable amounts of ni(ii) and cd(ii) in their growing and resting cells and showed g ...	2007	17512031
genetic diversity of acacia seyal del. rhizobial populations indigenous to senegalese soils in relation to salinity and ph of the sampling sites.	the occurrence and the distribution of rhizobial populations naturally associated to acacia seyal del. were characterized in 42 soils from senegal. the diversity of rhizobial genotypes, as characterized by polymerase chain reaction restriction fragment length polymorphism (rflp) analysis of 16s-23s rdna, performed on dna extracted from 138 nodules resulted in 15 clusters. results indicated the widespread occurrence of compatible rhizobia associated to a. seyal in various ecogeographic areas. how ...	2007	17406772
reduction of nickel and uranium toxicity and enhanced trichloroethylene degradation to burkholderia vietnamiensis pr1301 with hydroxyapatite amendment.	the use of hydroxyapatite (ha) to sequester metals at mixed waste sites may reduce metal toxicity and facilitate microbial degradation of cocontaminant organics. the constitutive trichloroethylene (tce) degrader, burkholderia vietnamiensis pr1301, grew at 34.1 and 1.7 mm ni at ph 5 and 7, respectively, with 0.01 g ml(-1) ha compared to 17 and 0.85 mm ni without ha. pr1 grew at 4.2 mm u at ph 5 and 7 with 0.01 g ml(-1) ha compared to 1.1 mm u without ha. a similar decrease in the toxicity of ni a ...	2007	17410778
endosymbiont-dependent host reproduction maintains bacterial-fungal mutualism.	bacterial endosymbionts play essential roles for many organisms, and thus specialized mechanisms have evolved during evolution that guarantee the persistence of the symbiosis during or after host reproduction. the rice seedling blight fungus rhizopus microsporus represents a unique example of a mutualistic life form in which a fungus harbors endobacteria (burkholderia sp.) for the production of a phytotoxin. here we report the unexpected observation that in the absence of endosymbionts, the host ...	2007	17412585
evaluation of preservative systems in opthalmic suspension of polymyxin b and dexamethasone by linear regression.	an ophthalmic suspension of polymyxin b and dexametasone with 13 different preservative systems was evaluated by a linear regression method for rapid screening, using d-values. the products were challenged against pseudomonas aeruginosa, burkholderia cepacia, staphylococcus aureus, candida albicans and aspergillus niger. since linear regression showed correlation coefficients higher than 0.84 the decimal reduction time (d-value) was then calculated. the comparison of d-values obtained for each m ...	2007	17416196
characterization of a c-c bond hydrolase from sphingomonas wittichii rw1 with novel specificities towards polychlorinated biphenyl metabolites.	sphingomonas wittichii rw1 degrades chlorinated dibenzofurans and dibenzo-p-dioxins via meta cleavage. we used inverse pcr to amplify dxnb2, a gene encoding one of three meta-cleavage product (mcp) hydrolases identified in the organism that are homologues of bphd involved in biphenyl catabolism. purified dxnb2 catalyzed the hydrolysis of 8-oh 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (hopda) approximately six times faster than for hopda at saturating substrate concentrations. moreover, the speci ...	2007	17416660
infection with burkholderia cepacia complex bacteria and pulmonary exacerbations of cystic fibrosis.	studies have shown that cystic fibrosis (cf) patients who are chronically infected with burkholderia cepacia complex bacteria may potentially acquire new strains of b cepacia. our objective was to determine whether pulmonary exacerbations of cf are associated with acquisition of new b cepacia strains or with b cepacia strain replacement.	2007	17426227
special considerations for patients with cystic fibrosis undergoing lung transplantation.	this article reviews lung transplantation in patients with cystic fibrosis (cf). lung transplantation is commonly utilized for patients with end-stage cf. there are several characteristics of cf that present unique challenges before and after lung transplantation. there is new information available that can be utilized to predict outcomes in patients with end-stage cf, and therefore can help in decisions of referral and listing for lung transplantation. the new lung allocation score, which alloc ...	2007	17426231
use of protein-specific monoclonal antibody-based latex agglutination for rapid diagnosis of burkholderia pseudomallei infection in patients with community-acquired septicemia.	a latex agglutination test employing monoclonal antibody specific to a 30-kda protein of burkholderia pseudomallei was used to detect the organisms in blood culture specimens from 1,139 patients with community-acquired septicemia. the sensitivity, specificity, and positive and negative predictive values of the test were 96.75%, 99.61%, 96.75%, and 99.61%, respectively.	2007	17428952
assessing the reliability of eburst using simulated populations with known ancestry.	the program eburst uses multilocus sequence typing data to divide bacterial populations into groups of closely related strains (clonal complexes), predicts the founding genotype of each group, and displays the patterns of recent evolutionary descent of all other strains in the group from the founder. the reliability of eburst was evaluated using populations simulated with different levels of recombination in which the ancestry of all strains was known.	2007	17430587
bactericidal action of positive and negative ions in air.	in recent years there has been renewed interest in the use of air ionisers to control of the spread of airborne infection. one characteristic of air ions which has been widely reported is their apparent biocidal action. however, whilst the body of evidence suggests a biocidal effect in the presence of air ions the physical and biological mechanisms involved remain unclear. in particular, it is not clear which of several possible mechanisms of electrical origin (i.e. the action of the ions, the p ...	2007	17439657
the tautomeric half-reaction of bphd, a c-c bond hydrolase. kinetic and structural evidence supporting a key role for histidine 265 of the catalytic triad.	bphd of burkholderia xenovorans lb400 catalyzes an unusual c-c bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (hopda) to afford benzoic acid and 2-hydroxy-2,4-pentadienoic acid (hpd). an enol-keto tautomerization has been proposed to precede hydrolysis via a gem-diol intermediate. the role of the canonical catalytic triad (ser-112, his-265, asp-237) in mediating these two half-reactions remains unclear. we previously reported that the bphd-catalyzed hydrolysis of hopda (lambda( ...	2007	17442675
use of immunoblotting as an alternative method for serogrouping leptospira.	leptospirosis is a worldwide zoonotic disease caused by a spirochaete bacterium, leptospira. serological detection of this micro-organism basically relies on a conventional microscopic agglutination test (mat), which has some limitations and disadvantages. in the present study, immunoblotting has been applied as an alternative method for differentiating serogroups and serovars of leptospires. leptospiral whole-cell lysates from a total of 26 serovars were subjected to immunoblotting using rabbit ...	2007	17446278
bacteria-associated haemophagocytic syndrome and septic pulmonary embolism caused by burkholderia cepacia complex in a woman with chronic granulomatous disease.	chronic granulomatous disease (cgd) is a primary immunodeficiency characterized by recurrent infections with certain types of bacteria and fungi. presented herein is the case of a 29 year old woman with cgd who suffered from bacteria-associated haemophagocytic syndrome and a septic pulmonary embolism following a uterine infection and sepsis, caused by burkholderia cepacia complex.	2007	17446299
melioidotic septic arthritis: a case report and literature review.	burkholderia pseudomallei, the causative agent of melioidosis, is endemic in southeast asia and northern australia. in recent years, the incidence of melioidosis has increased worldwide. septic arthritis is a rare but well-recognized manifestation of melioidosis. patients with underlying medical conditions, such as diabetes mellitus, renal impairment, cirrhosis, and malignancy are at greater risk. the presentations of melioidotic septic arthritis often mimic other disease processes and patients ...	2007	17446969
high genotypic diversity of pseudomonas aeruginosa strains isolated from patients with cystic fibrosis in the czech republic.	over the last decade, epidemic and frequently multidrug-resistant pseudomonas aeruginosa has increasingly been found among european cystic fibrosis (cf) patients. in the czech republic, more than half of the registered cf patients attend the prague cf centre. at this centre, a burkholderia cenocepacia strain was recently found to have spread among the patients. the aim of the present study was to assess whether p. aeruginosa isolates from patients at this centre were also genetically related and ...	2007	17448645
mesorhizobium spp. are the main microsymbionts of caragana spp. grown in liaoning province of china.	caragana species are woody legumes widely distributed in the arid regions of china. these plants form root nodules but their nodule bacteria have not been clearly classified. a total of 112 symbiotic bacterial isolates were obtained from four caragana species grown in liaoning province and were characterized with ardra, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins, box-pcr and sequencing of the 16s rrna gene. most of them were classified as mesorhizobium belonging to 11 ...	2007	17451445
atypical mycobacterial infection in patients with cystic fibrosis: update on clinical microbiology methods.	while patients with cystic fibrosis (cf) have had dramatic improvement in their survival rates, this has been accompanied by the emergence of more virulent pathogens such as pseudomonas aeruginosa and burkholderia cepacia complex organisms. in addition, there has been emergence of organisms of increasing clinical significance such as the nontuberculous mycobacterial (ntm). although tb infection in patients with cf is extremely uncommon, there is growing concern with regard to atypical mycobacter ...	2007	17451510
melioidosis complicated by pericarditis.	melioidosis is a bacterial infection caused by the gram-negative bacillus burkholderia pseudomallei. we report an unusual case of melioidosis that presented as a pyrexia of unknown origin complicated by pericardial effusion. our patient received a 6-week course of intravenous antibiotics, followed by 8 months of oral antibiotics, and made a complete recovery. this report illustrates the diagnostic and therapeutic challenge that clinicians may encounter when faced with this potentially fatal infe ...	2007	17454903
clinical and environmental burkholderia strains: biofilm production and intracellular survival.	bacteria belonging to the burkholderia species are important pulmonary pathogens in cystic fibrosis (cf) patients. their ability to establish chronic and sometimes fatal infections seems linked to the quorum sensing-regulated expression of virulence factors. we examined 23 burkholderia isolates, 19 obtained from cf patients and 4 from the environment, to evaluate their ability to form biofilm and to penetrate and replicate inside j774 macrophagic cells. our results indicate that biofilm formatio ...	2007	17457645
characteristics of phenol biodegradation in saline solutions by monocultures of pseudomonas aeruginosa and pseudomonas pseudomallei.	phenol is a highly toxic and carcinogenic compound and its biodegradation is very important to meet the environmental regulations. two bacterial strains capable of utilizing phenol as a sole source of carbon were isolated from the wastewater of a pharmaceutical industry. on the basis of morphological and biochemical characteristics these strains were identified as pseudomonas aeruginosa and pseudomonas pseudomallei. both of these strains were very efficient for phenol degradation. p. pseudomalle ...	2007	17459580
assessment of fluorescent in situ hybridization and pcr-based methods for rapid identification of burkholderia cepacia complex organisms directly from sputum samples.	several species within the burkholderia cepacia complex (bcc) have emerged as significant opportunistic pathogens of patients with cystic fibrosis (cf). bcc infection is typically associated with a poor clinical prognosis and decreased survival. these factors, combined with the existence of highly transmissible epidemic strains, have resulted in strict segregation of bcc- and non-bcc-infected patients to minimize cross infection. accurate and rapid diagnosis of infections is essential to enable ...	2007	17460050
ferripyochelin uptake genes are involved in pyochelin-mediated signalling in pseudomonas aeruginosa.	in response to iron starvation, pseudomonas aeruginosa produces the siderophore pyochelin. when secreted to the extracellular environment, pyochelin chelates iron and transports it to the bacterial cytoplasm via its specific outer-membrane receptor fpta and the inner-membrane permease fptx. exogenously added pyochelin also acts as a signal which induces the expression of the pyochelin biosynthesis and uptake genes by activating pchr, a cytoplasmic regulatory protein of the arac/xyls family. the ...	2007	17464065
hexadecane and tween 80 stimulate lipase production in burkholderia glumae by different mechanisms.	burkholderia glumae strain pg1 produces a lipase of biotechnological relevance. lipase production by this strain and its derivative lu8093, which was obtained through classical strain improvement, was investigated under different conditions. when 10% hexadecane was included in the growth medium, lipolytic activity in both strains could be increased approximately 7-fold after 24 h of growth. hexadecane also stimulated lipase production in a strain containing the lipase gene fused to the tac promo ...	2007	17468265
competitive fitness of isolates enriched on phenanthrene sorbed to model phases.	we evaluated the competitive fitness of three phenanthrene-mineralizing isolates that were provided with nonsorbed or sorbed phenanthrene in competition experiments. one isolate had a clear advantage when presented with amberlite irc-50-sorbed phenanthrene, yet none were favored when presented with nonsorbed or amberlite xad-7-sorbed phenanthrene. our results indicate that contaminant availability alone does not determine the isolates' competitive fitness.	2007	17468288
predictors of mortality in adults with cystic fibrosis.	assessment of prognostic indicators in patients with cystic fibrosis (cf) is important. the study's aim was to assess the relative contribution of gender, genetics and microbiology on survival in adults with cf. adult patients were studied from 1995 to 2005 and data collected included fev(1) (%predicted), body mass index (bmi), genetics, and microbiology. data was available on 183 patients in 1995. forty-five patients died in the subsequent 10 years. patients who died during the study had lower ...	2007	17469153
selection of nitrogen-fixing deficient burkholderia vietnamiensis strains by cystic fibrosis patients: involvement of nif gene deletions and auxotrophic mutations.	burkholderia vietnamiensis is the third most prevalent species of the burkholderia cepacia complex (bcc) found in cystic fibrosis (cf) patients. its ability at fixing nitrogen makes it one of the main bcc species showing strong filiations with environmental reservoirs. in this study, 83% (29 over 35) of the b. vietnamiensis cf isolates and 100% of the environmental ones (over 29) were found expressing the dinitrogenase complex (encoded by the nif cluster) which is essential in n(2) fixation. amo ...	2007	17472633
molecular detection and diversity of novel diterpenoid dioxygenase dita1 genes from proteobacterial strains and soil samples.	resin acids are tricyclic diterpenoids naturally synthesized by trees that are released from wood during pulping processes. using a newly designed primer set, genes similar to that encoding the dita1 catalytic alpha-subunit of the diterpenoid dioxygenase, a key enzyme in abietane resin acid degradation by pseudomonas abietaniphila bkme-9, could be amplified from different pseudomonas strains, whereas dita1 gene sequence types representing distinct branches in the evolutionary tree were amplified ...	2007	17472635
burkholderia nodosa sp. nov., isolated from root nodules of the woody brazilian legumes mimosa bimucronata and mimosa scabrella.	three strains, br3437(t), br3461 and br3470, were isolated from nitrogen-fixing nodules on the roots of mimosa scabrella (br3437(t)) and mimosa bimucronata (br3461, br3470), both of which are woody legumes native to brazil. on the basis of 16s rrna gene sequence similarities, all the strains were shown previously to belong to the genus burkholderia. a polyphasic approach, including dna-dna hybridizations, pfge of whole-genome dna profiles, whole-cell protein analyses, fatty acid methyl ester ana ...	2007	17473258
liver abscess caused by tuberculosis and melioidosis.	we report an unusual co-existence of burkholderia pseudomallei and acid fast bacilli in a young malay gentleman with liver abscess. he was treated with antibiotics and surgical drainage. this phenomenon has not been reported in previous literature and the dilemma of its management is discussed.	2007	17475585
misdiagnosing melioidosis.		2007	17479916
insect-microbe mutualism without vertical transmission: a stinkbug acquires a beneficial gut symbiont from the environment every generation.	the broad-headed bug riptortus clavatus (heteroptera: alydidae) possesses a number of crypts at a posterior midgut region, which house a dense population of a bacterial symbiont belonging to the genus burkholderia. although the symbiont is highly prevalent (95 to 100%) in the host populations, the symbiont phylogeny did not reflect the host systematics at all. in order to understand the mechanisms underlying the promiscuous host-symbiont relationship despite the specific and prevalent associatio ...	2007	17483286
the bryophyte genus sphagnum is a reservoir for powerful and extraordinary antagonists and potentially facultative human pathogens.	sphagnum plants grow in natural, species-poor carpets at low ph but without any known substantial fungal disease. to investigate this phenomenon, we analysed bacterial populations associated with two sphagnum species with different ecological behaviour, namely s. magellanicum and s. fallax, from three sites in germany and three in norway, with a special focus on the functional group of antagonists. the screening of 493 bacterial isolates for antagonistic activity against fungal pathogens resulte ...	2007	17484734
salvage treatment of pneumonia and initial treatment of tracheobronchitis caused by multidrug-resistant gram-negative bacilli with inhaled polymyxin b.	systemic colistin has shown efficacy against multidrug-resistant pseudomonas aeruginosa and acinetobacter spp., but it has presented poor results in pneumonia. aerosolized polymyxin in cystic fibrosis patients has had good results. in this study, inhaled polymyxin b was used to treat respiratory infections by multidrug-resistant gram-negative bacilli (mr-gnbs). nineteen patients were treated with inhaled polymyxin b: 14 pneumonia, most of which had previously failed treatment with intravenous po ...	2007	17350201
burkholderia pseudomallei animal and human isolates from malaysia exhibit different phenotypic characteristics.	burkholderia pseudomallei is a gram-negative saprophytic soil bacterium, which is the etiologic agent of melioidosis, a severe and fatal infectious disease occurring in human and animals. distinct clinical and animal isolates have been shown to exhibit differences in phenotypic trait such as growth rate, colony morphology, antimicrobial resistance, and virulence. this study was carried out to gain insight into the intrinsic differences between 4 clinical and 6 animal b. pseudomallei isolates fro ...	2007	17350202
quorum-sensing signals produced by plant-growth promoting burkholderia strains under in vitro and in planta conditions.	the genus burkholderia is a heterogeneous group with extraordinary nutritional versatility and which occupies a diversity of niches. in recent decades, members of burkholderia have been shown to be active participants in plant-microbe interactions, imparting beneficial effects as plant-growth-promoting bacteria (pgpb) or as pathogens. the study of quorum sensing and cell-density-dependent gene regulation, which play an important role in host colonization and pathogenesis, is extremely important ...	2007	17350232
effect of the mycorrhizosphere on the genotypic and metabolic diversity of the bacterial communities involved in mineral weathering in a forest soil.	to date, several bacterial species have been described as mineral-weathering agents which improve plant nutrition and growth. however, the possible relationships between mineral-weathering potential, taxonomic identity, and metabolic ability have not been investigated thus far. in this study, we characterized a collection of 61 bacterial strains isolated from scleroderma citrinum mycorrhizae, the mycorrhizosphere, and the adjacent bulk soil in an oak forest. the ability of bacteria to weather bi ...	2007	17351101
proteomic analysis of burkholderia cepacia mba4 in the degradation of monochloroacetate.	burkholderia cepacia mba4 is a bacterium that degrades 2-haloacids by removing the halogen and subsequent metabolism of the product for energy. in this study, 2-de, ms/ms, and n-terminal amino acid sequencing were used to investigate the protein expression profiles of mba4 grown in a 2-haloacid (monochloroacetate, mca) and in the corresponding metabolic product (glycolate). glycolate was used as a control to eliminate the proteins induced by it. five proteins were found to be up-regulated and fi ...	2007	17352424
identifying the genetic basis of ecologically and biotechnologically useful functions of the bacterium burkholderia vietnamiensis.	signature-tagged mutagenesis (stm) was used to identify genetic determinants of fitness associated with two key ecological processes mediated by bacteria. burkholderia vietnamiensis strain g4 was used as a model bacterium to investigate: phenol degradation as a model of bioremediation, and pea rhizosphere colonization as a prerequisite to biological control and phytoremediation. a total of 1900 mutants were screened and 196 putative fitness mutants identified; the genetic basis of 137 of these m ...	2007	17359273
development and clinical evaluation of a pcr assay targeting the metalloprotease gene (mpra) of b. pseudomallei.	a pcr assay targeting the metalloprotease gene (mpra) of burkholderia pseudomallei was developed for the specific detection of this organism in pure cultures and clinical samples. all other closely related organisms including b. mallei the causative agent of glanders, and b. thailandensis tested negative. burkholderia pseudomallei dna was successfully amplified from paraffin-embedded lung tissue of a camel with a generalized b. pseudomallei infection. the developed pcr assay can be used as a sim ...	2007	17359446
occurrence of burkholderia cepacia complex, ralstonia and pandoraea species dnas in the coastal environment of the straits of messina (italy).		2007	17360005
exceptionally high representation of burkholderia cepacia among b. cepacia complex isolates recovered from the major portuguese cystic fibrosis center.	burkholderia cepacia, a species found infrequently in cystic fibrosis (cf), was isolated from 85% of patients infected with bacteria of the b. cepacia complex that visited the major portuguese cf center, in lisbon, during 2003 to 2005. a detailed molecular analysis revealed that this was mainly due to two b. cepacia clones. these clones were indistinguishable from two strains isolated from intrinsically contaminated nonsterile saline solutions for nasal application, detected during routine marke ...	2007	17360834
evaluation of the wayson stain for the rapid diagnosis of melioidosis.		2007	17360835
chromate reduction by burkholderia cepacia mcmb-821, isolated from the pristine habitat of alkaline crater lake.	the cr(vi)-reducing bacterial strain mcmb-821 was isolated from the alkaline crater lake of lonar and was identified as burkholderia cepacia. mcmb-821 was resistant to 1,000-ppm cr(vi) and reduced 98% of the 75 ppm cr(vi) within 36 h at ph 9.0 in the presence of 2% salt and lactose as the electron donor. the chromate-reducing efficiency of mcmb-821 was comparable under both aerobic as well as anaerobic conditions. electron paramagnetic resonance spectroscopy data suggested that mcmb-821 reduced ...	2007	17361433
burkholderia hep_hag autotransporter (buha) proteins elicit a strong antibody response during experimental glanders but not human melioidosis.	the bacterial biothreat agents burkholderia mallei and burkholderia pseudomallei are the cause of glanders and melioidosis, respectively. genomic and epidemiological studies have shown that b. mallei is a recently emerged, host restricted clone of b. pseudomallei.	2007	17362501
crystal structures of the substrate free-enzyme, and reaction intermediate of the had superfamily member, haloacid dehalogenase dehiva from burkholderia cepacia mba4.	dehiva is a haloacid dehalogenase (ec 3.8.1.2) from the soil and water borne bacterium burkholderia cepacia mba4, which belongs to the functionally variable haloacid dehalogenase (had) superfamily of enzymes. the haloacid dehalogenases catalyse the removal of halides from haloacids resulting in a hydroxlated product. these enzymes are of interest for their potential to degrade recalcitrant halogenated environmental pollutants and their use in the synthesis of industrial chemicals. the haloacid d ...	2007	17368477
characterization of a bifunctional catalase-peroxidase of burkholderia cenocepacia.	isolates of burkholderia cenocepacia express a putative haem-binding protein (molecular mass 97 kda) that displays intrinsic peroxidase activity. its role has been re-evaluated, and we now show that it is a bifunctional catalase-peroxidase, with activity against tetramethylbenzidine (tmb), o-dianisidine, pyrogallol, and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic) acid (abts). both peroxidase and catalase activities are optimal at ph 5.5-6.0. the gene encoding this enzyme was cloned and exp ...	2007	17371508
high-throughput mrna profiling characterizes the expression of inflammatory molecules in sepsis caused by burkholderia pseudomallei.	sepsis is characterized by an uncontrolled inflammatory response to invading microorganisms. we describe the inflammatory mrna profiles in whole-blood leukocytes, monocytes, and granulocytes using a multigene system for 35 inflammatory markers that included pro- and anti-inflammatory cytokines, chemokines, and signal transduction molecules in a case-control study with 34 patients with sepsis caused by the gram-negative bacterium burkholderia pseudomallei (the pathogen causing melioidosis) and 32 ...	2007	17371859
metabolic and phylogenetic analysis of microbial communities during phytoremediation of soil contaminated with weathered hydrocarbons and heavy metals.	in the current study, the microbial ecology of weathered hydrocarbon and heavy metal contaminated soil undergoing phytoremediation was studied. the relationship of functional diversity, measured as carbon source utilisation in biolog plates and extracellular enzymatic activities, and genetic diversity of bacteria was evaluated. denaturing gradient gel electrophoresis was used for community analyses at the species level. bulk soil and rhizosphere soil from pine and poplar plantations were analyse ...	2007	17372705
motility and chemotaxis of pseudomonas sp. b4 towards polychlorobiphenyls and chlorobenzoates.	the polychlorinated biphenyl (pcb)-degrading pseudomonas sp. b4 was tested for its motility and ability to sense and respond to biphenyl, its chloroderivatives and chlorobenzoates in chemotaxis assays. pseudomonas sp. b4 was attracted to biphenyl, pcbs and benzoate in swarm plate and capillary assays. chemotaxis towards these compounds correlated with their use as carbon and energy sources. no chemotactic effect was observed in the presence of 2- and 3-chlorobenzoates. furthermore, a toxic effec ...	2007	17374130
microbial ecology of the cystic fibrosis lung.	understanding the microbial flora of the cystic fibrosis (cf) respiratory tract is of considerable importance, as patient morbidity and death are primarily caused by chronic respiratory infections. however, chronically colonized cf airways represent a surprisingly complex and diverse ecosystem. the precise contributions of different microbes to patient morbidity, and in particular the importance of inter-specific interactions, remain largely unelucidated. the importance of within-species genetic ...	2007	17379702
to catch a killer gene.		2007	17380132
drosophila eiger mutants are sensitive to extracellular pathogens.	we showed previously that eiger, the drosophila tumor necrosis factor homolog, contributes to the pathology induced by infection with salmonella typhimurium. we were curious whether eiger is always detrimental in the context of infection or if it plays a role in fighting some types of microbes. we challenged wild-type and eiger mutant flies with a collection of facultative intracellular and extracellular pathogens, including a fungus and gram-positive and gram-negative bacteria. the response of ...	2007	17381241
enumeration, isolation and identification of diazotrophs from korean wetland rice varieties grown with long-term application of n and compost and their short-term inoculation effect on rice plants.	this study has been aimed (i) to isolate and identify diazotrophs from korean rice varieties; (ii) to examine the long-term effect of n and compost on the population dynamics of diazotrophs and (iii) to realize the shot-term inoculation effect of these diazotrophs on rice seedlings.	2007	17381741
control of quorum sensing by a burkholderia pseudomallei multidrug efflux pump.	the burkholderia pseudomallei khw quorum-sensing systems produced n-octanoyl-homoserine lactone, n-decanoyl-homoserine lactone, n-(3-hydroxy)-octanoyl-homoserine lactone, n-(3-hydroxy)-decanoyl-homoserine lactone, n-(3-oxo)-decanoyl-homoserine lactone, and n-(3-oxo)-tetradecanoyl-homoserine lactone. the extracellular secretion of these acyl-homoserine lactones is dependent absolutely on the function of the b. pseudomallei bpeab-oprb efflux pump.	2007	17384185
paediatric melioidosis with septic shock in a previously-well child.	we present a previously-healthy 12-year old girl from a rural community and who was admitted to a district general hospital in malaysia with coagulopathy and septic shock. despite receiving intensive care, she succumbed to her illness. blood cultures grew burkholderia pseudomallei. melioidosis is an unusual cause of paediatric gram-negative sepsis among children in malaysia.	2007	17384864
pathogens in early-onset and late-onset intensive care unit-acquired pneumonia.	to compare the type of pathogens isolated from patients with early-onset intensive care unit (icu)-acquired pneumonia with those isolated from patients with late-onset icu-acquired pneumonia and to study risk factors for the isolation of pathogens that are potentially resistant to multiple drugs.	2007	17385143
in vitro antimicrobial activity of the leaf extract of harungana madagascariensis lam. ex poir. (hypericaceae) against strains causing otitis externa in dogs and cats.	otitis externa in dogs and cats is always caused by a combination of yeasts and bacteria, among which the most important are malassezia pachydermatis, staphylococcus intermedius and pseudomonas species. these organisms often develop resistance to classical antimicrobial agents. therefore, the aim of this study was to investigate the antimicrobial activities of an ethyl acetate leaf extract of harungana madagascariensis against the organisms cited, to carry out the phytochemical investigation of ...	2007	17385560
atp-binding cassette systems in burkholderia pseudomallei and burkholderia mallei.	atp binding cassette (abc) systems are responsible for the import and export of a wide variety of molecules across cell membranes and comprise one of largest protein superfamilies found in prokarya, eukarya and archea. abc systems play important roles in bacterial lifestyle, virulence and survival. in this study, an inventory of the abc systems of burkholderia pseudomallei strain k96243 and burkholderia mallei strain atcc 23344 has been compiled using bioinformatic techniques.	2007	17391530
human single-chain fv antibodies against burkholderia mallei and burkholderia pseudomallei.	much effort has been devoted to the development of mouse monoclonal antibodies that react specifically with burkholderia mallei and burkholderia pseudomallei for diagnostic and/or therapeutic purposes. our present study focused on the screening of a phage-displayed nonimmune human single-chain fv (scfv) antibody library against heat-killed b. mallei and b. pseudomallei for the generation of human scfv antibodies specific to the two pathogenic species of bacteria. using two different panning proc ...	2007	17392491
tandem repeat regions within the burkholderia pseudomallei genome and their application for high resolution genotyping.	the facultative, intracellular bacterium burkholderia pseudomallei is the causative agent of melioidosis, a serious infectious disease of humans and animals. we identified and categorized tandem repeat arrays and their distribution throughout the genome of b. pseudomallei strain k96243 in order to develop a genetic typing method for b. pseudomallei. we then screened 104 of the potentially polymorphic loci across a diverse panel of 31 isolates including b. pseudomallei, b. mallei and b. thailande ...	2007	17397553
comprehensive analysis of distinctive polyketide and nonribosomal peptide structural motifs encoded in microbial genomes.	we developed a highly accurate method to predict polyketide (pk) and nonribosomal peptide (nrp) structures encoded in microbial genomes. pks/nrps are polymers of carbonyl/peptidyl chains synthesized by polyketide synthases (pks) and nonribosomal peptide synthetases (nrps). we analyzed domain sequences corresponding to specific substrates and physical interactions between pkss/nrpss in order to predict which substrates (carbonyl/peptidyl units) are selected and assembled into highly ordered chemi ...	2007	17400247
monochloramine inactivation of bacterial select agents.	seven species of bacterial select agents were tested for susceptibility to monochloramine. under test conditions, the monochloramine routinely maintained in potable water would reduce six of the species by 2 orders of magnitude within 4.2 h. bacillus anthracis spores would require up to 3.5 days for the same inactivation with monochloramine.	2007	17400782
synthesis of highly hydroxylated aromatics by evolved biphenyl dioxygenase and subsequent dihydrodiol dehydrogenase.	the evolved bpha1 (2049) gene, in which nine amino acids from the pseudomonas pseudoalcaligenes kf707 bpha1 were changed to those from the burkholderia xenovorans lb400 bpha1 (m247i, h255q, v258i, g268a, d303e, -313g, s324t, v325i, and t376n), was expressed in escherichia coli along with the bpha2a3a4 and bphb genes derived from strain kf707. this recombinant e. coli cells converted biphenyl and several heterocyclic aromatic compounds into the highly hydroxylated products such as biphenyl-2,3,2' ...	2007	17401562
imported melioidosis with an isolated cutaneous presentation in a 90-year-old traveller from bangladesh.	melioidosis is a tropical disease caused by infection with the bacterium burkholderia pseudomallei. most cases present as an acute febrile illness with severe pneumonia and sepsis. sub-acute and late onset disease can also occur melioidosis has been diagnosed among travellers who contracted the disease while staying in endemic areas during the rainy season. we report a case of travel-associated b. pseudomallei cutaneous infection in a febrile 90-year-old woman with diabetes mellitus, with early ...	2007	17402688
the type iv pilin of burkholderia mallei is highly immunogenic but fails to protect against lethal aerosol challenge in a murine model.	burkholderia mallei is the cause of glanders and a proven biological weapon. we identified and purified the type iv pilin protein of this organism to study its potential as a subunit vaccine. we found that purified pilin was highly immunogenic. furthermore, mice infected via sublethal aerosol challenge developed significant increases in titers of antibody against the pilin, suggesting that it is expressed in vivo. nevertheless, we found no evidence that high-titer antipilin antisera provided pas ...	2007	17403869
redox and functional analysis of the rieske ferredoxin component of the toluene 4-monooxygenase.	toluene 4-monooxygenase catalyzes the nadh- and o2-dependent hydroxylation of toluene to form p-cresol. the four-protein complex consists of a diiron hydroxylase, an oxidoreductase, a catalytic effector protein, and a rieske-type ferredoxin (t4moc). phylogenetic analysis suggests that t4moc is part of a clade specialized for reaction with diiron hydroxylases, possibly reflected in the conservation of w69, whose indole side chain makes close contacts with a bridging sulfide. in order to further i ...	2007	17240981
identification of burkholderia pseudomallei and related bacteria by multiple-locus sequence typing-derived pcr and real-time pcr.	close relatedness and genomic plasticity characterizing the high-threat pathogens burkholderia pseudomallei and burkholderia mallei render the molecular diagnosis of these species hard to guarantee with a maximal confidence level. this article describes fast molecular assays derived from compiled sequences of housekeeping genes determined in more than 1,000 strains. the assays proved to be robust and appropriate for general detection as well as species identification purposes.	2007	17251403
investigation of a general base mechanism for ester hydrolysis in c-c hydrolase enzymes of the alpha/beta-hydrolase superfamily: a novel mechanism for the serine catalytic triad.	previous mechanistic and crystallographic studies on two c-c hydrolase enzymes, escherichia coli mhpc and burkholderia xenovorans bphd, support a general base mechanism for c-c hydrolytic cleavage, rather than the nucleophilic mechanism expected for a serine hydrolase. the role of the active site serine residue could be to form a hydrogen bond with a gem-diolate intermediate, or to protonate such an intermediate. hydrolase bphd is able to catalyse the hydrolysis of p-nitrophenyl benzoate ester s ...	2007	17252134
biodegradation of alpha- and beta-endosulfan by soil bacteria.	extensive applications of persistent organochlorine pesticides like endosulfan on cotton have led to the contamination of soil and water environments at several sites in pakistan. microbial degradation offers an effective approach to remove such toxicants from the environment. this study reports the isolation of highly efficient endosulfan degrading bacterial strains from soil. a total of 29 bacterial strains were isolated through enrichment technique from 15 specific sites using endosulfan as s ...	2007	17252313
vntr analysis of selected outbreaks of burkholderia pseudomallei in australia.	molecular typing methods for burkholderia pseudomallei have been successful at assigning isolates into epidemiologically related groups, but have not been able to detect differences and define evolutionary patterns within groups. our variable number tandem repeat (vntr) analysis of a set of 121 australian b. pseudomallei isolates, 104 of which were associated with nine epidemiological groups, provides fine scale differentiation even among very closely related isolates. we used a bayesian model b ...	2007	17258514
redox intermediates in the catalase cycle of catalase-peroxidases from synechocystis pcc 6803, burkholderia pseudomallei, and mycobacterium tuberculosis.	monofunctional catalases (ec 1.11.1.6) and catalase-peroxidases (katgs, ec 1.11.1.7) have neither sequence nor structural homology, but both catalyze the dismutation of hydrogen peroxide (2h2o2 --> 2h2o + o2). in monofunctional catalases, the catalatic mechanism is well-characterized with conventional compound i [oxoiron(iv) porphyrin pi-cation radical intermediate] being responsible for hydrogen peroxide oxidation. the reaction pathway in katgs is not as clearly defined, and a comprehensive rap ...	2007	17260948
comparison of antibiotic susceptibility of burkholderia cepacia complex organisms when grown planktonically or as biofilm in vitro.	this study determined the antibiotic susceptibility of planktonic and biofilm cultures of burkholderia cepacia complex organisms, a group of highly problematic pathogens associated with cystic fibrosis patients. the biofilm inhibitory concentrations were considerably higher than the corresponding minimum inhibitory concentrations for meropenem and piperacillin-tazobactam. however, tobramycin and amikacin were efficacious against both biofilm and planktonic cultures. overall this study showed tha ...	2007	17265071