Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| clostridium infection in mother and infant. | 1980 | 6255793 | |
| relationship between metronidazole metabolism and bactericidal activity. | it has been suggested that the microbicidal effect of metronidazole is mediated by an intermediate in nitro group reduction. we have found that the addition of escherichia coli enhances the lethal effect of metronidazole on bacillus fragilis and suggest that this intermediate may form in one bacteria and kill another. because acetamide forms during the reduction of metronidazole, we examined the possibility that the same partially reduced intermediate in metronidazole reduction may be both an in ... | 1980 | 6255861 |
| preliminary report on serogrouping of indian strains of clostridium perfringens type a. | 1980 | 6256288 | |
| mechanisms of pathogenicity of anaerobic bacteria of clinical interest. | the host factors leading to anaerobic infection and the various models of anaerobic infection, namely direct effect of a single toxin, multiple toxin-aggressin-systems, enterotoxic mechanisms, endogenous infections with non-clostridial anaerobes, and synergistic infections, are reviewed. production of carcinogens and co-carcinogens by anaerobes is also discusssed. | 1980 | 6256292 |
| methods for testing antibiotic sensitivity of anaerobic bacteria. | problems are still encountered in the performance and interpretation of tests of anaerobe sensitivity to antibiotics. a review of the methods currently used was carried out in order to determine factors modifying the activity of antibiotics. the sensitivity of escherichia coli, staphylococcus aureus and clostridium perfringens to various drugs was tested under different conditions (including different culture media and incubation atmospheres). gentamicin and kanamycin showed no activity in tests ... | 1980 | 6256293 |
| the bacteriological quality of minced beef in the u.k. | minced (ground) beef from three supermarkets, three intermediate-sized chain butchers and three small family butchers in each of three geographical areas was examined three times in warm weather and three times in cool. the total viable count (37 and 20 degrees c), numbers of enterobacteriaceae (37 and 17 degrees c), and presumptive coliforms did not differ significantly between shop type or season. statistically significant differences in numbers of faecal streptococci, staphylococcus aureus an ... | 1980 | 6256434 |
| [susceptibility of anaerobic bacteria to cefoxitin (author's transl)]. | nine strains of clostridium perfringens type a and 10 strains of bacteroïdes fragilis s. spec. fragilis, recently isolated from blood culture, were tested for their cefoxitin susceptibility. the mic for clostridium perfringens ranged from 0,6 to 5 mg/l and 1,2 to 10 mg/l for bacteroïdes fragilis. in most cases, the mbc were identical with the mic for clostridium perfringens, but for bact. frag. there was a difference of 2 dilutions in their mbc. cefoxitin activity was not modified by a variation ... | 1980 | 6256880 |
| behavior of pathogenic bacteria in the oyster, crassostrea commercialis, during depuration, re-laying, and storage. | oysters (crassostrea commercials) harvested from major cultivation areas within the state of new south wales, australia, were commonly contaminated with low levels of the food-poisoning organisms bacillus cereus, clostridium perfringens, and vibrio parahaemolyticus. salmonella was found in oysters on only one occasion. these bacteria were cleansed from oysters during oyster purification by re-laying in a non-polluted waterway. oysters were laboratory contaminated to levels in excess 1,000 cells ... | 1980 | 6257164 |
| plasmid detection in a bacteriocinogenic strain of clostridium perfringens. | bacteriocinogenic clostridium perfringens, strain 28, harboured plasmid dna detectable by dye-bouyant density-gradient centrifugation. this plasmid dna was absent from an ultraviolet light cured variant which had simultaneously lost its immunity and ability to produce bacteriocin. agarose gel electrophoresis of the plasmid dna revealed at least six bands but denaturation experiments suggested three plasmids occurring in more than one conformation. electron microscopy revealed three major size di ... | 1980 | 6257358 |
| ultrastructure of sporulating cells of clostridium perfringens type a grown in the presence of raffinose. | seven-hour sporulating cells of clostridium perfringens type a strain nctc 10240 grown in the presence of raffinose versus starch were larger, produced a thicker cortex, and were altered in spore coat structure. strain nctc 8798 possessed an expanded subcoat region and (or) produced coat fragments when grown in the presence of raffinose. | 1980 | 6257363 |
| a survey of clostridium perfringens enterotoxin antibody in human and animal sera in western canada. | sera from human, cattle, sheep, swine, and horse populations in western canada were tested for the presence of clostridium perfringens enterotoxin antibody by the passive hemagglutination (pha) test, supplemented by an immunodiffusion test and by counterimmunoelectrophoresis. a total of 224 human, 345 cattle, 165 sheep, 620 swine, and 768 horse serum samples were examined. low-titer reactions in the pha test were detected in human, cattle, horse, and swine sera, in that order, with no titers dem ... | 1980 | 6257364 |
| the protection role of pyruvate against heat inactivation of n-acetylneuraminate lyase. | 1980 | 6257549 | |
| clostridium perfringens type a enteropathy: studies with a nonenterotoxigenic strain. | 1980 | 6257612 | |
| radioiodination of enterotoxin from clostridium perfringens type a using chloramine t. | 1980 | 6257632 | |
| passive bacteriocin typing of strains of clostridium perfringens type a causing food poisoning for epidemiologic studies. | a passive bacteriocin typing system was developed for use as an epidemiologic tool to study outbreaks of food poisoning caused by clostridium perfringens type a. the 90 strains tested were from england, the united states, india, japan, france, and canada. forty of 74 strains produced bacteriocin when irradiated with ultraviolet light for 40-90 sec. eight bacteriocins were concentrated with ammonium sulfate and titrated on a common indicator strain. these bacteriocins were found to be specific fo ... | 1980 | 6257802 |
| enterotoxaemia involving clostridium perfringens iota toxin in a hysterectomy-derived rabbit colony. | during an explosive outbreak of fatal enteropathic disease involving clostridium perfringens iota (i) toxin, a total of 183 deaths occurred in 18 weeks. the clinical signs and post-mortem findings are reported. examinations for virus, bacillus piliformis and coccidia were negative. clostridium perfringens i toxin was detected in 22 of 27 animals examined (81.5%), but clostridia were not isolated. various treatments were attempted. it is concluded that i toxin and the syndrome described are close ... | 1980 | 6257969 |
| [post-operative meningitis due to obligatory anaerobic bacteria: 3 case reports]. | 1980 | 6258136 | |
| [the suitability of bioindicators according to din 58948 part 4 for monitoring gas-sterilizers (author's transl)]. | in the federal republic of germany bioindicators according to din 58948, part 4, are generally used for testing the efficacy of ethylene oxide sterilizers. they are based on spores of bacillus subtilis dried in sheep-blood on the bottom of a small test tube. as some authors doubted the resistance of these indicators to ethylene oxide especially in comparison with spore soil and spores of clostridium perfringens, three different test procedures were performed showing that spores of sulfite reduci ... | 1980 | 6258352 |
| demonstration and characterization of partial glyceride specific lipases in pig thyroid plasma membranes. | 1980 | 6258596 | |
| mechanism of nitrite-induced germination of clostridium perfringens spores. | 1980 | 6260726 | |
| bacteriophage typing of food poisoning strains of c. perfringens type a. | 1980 | 6262229 | |
| necrotising enteritis--a review. | 1980 | 6262230 | |
| [drinking water of madagascar (7,891 bacteriological analyses--1970-1977)]. | 1980 | 6263199 | |
| [drinking water of madagascar from 1959 to 1977 (20,465 bacteriological analyses)]. | 1980 | 6263200 | |
| [food bacteriology in madagascar. laboratory activities from 1965 to 1977. techniques and results]. | 1980 | 6263201 | |
| role of the intestinal flora in the metabolism of misonidazole. | 1980 | 6260109 | |
| [microbial flora of beef extract imported from australia, uruguay, italy and brazil (author's transl)]. | 1980 | 6265014 | |
| active perfringocin typing of food poisoning strains of clostridium perfringens type a--a new tool for epidemiological investigations. | active perfringocin typing, as an epidemiological tool, for investigation into food poisoning outbreaks due to c. perfringens type a has been developed. ninety strains included in this study were from england, america, india, japan, france and canada nine indicator strains exhibiting different patterns of inhibition were selected for perfringocin typing. with these indicator strains, 81.11 per cent of the strains were typable into ten perfringocin types. type "a" and "b" alone could type 47.92 p ... | 1980 | 6265390 |
| immunological detection of food-poisoning toxins. | 1980 | 6269224 | |
| comparison of metronidazole assay by microbiological and chemical methods. | chemical (thin layer chromatography/fluorescence quenching in situ) and microbiological (agar well, diffusion technique with clostridium perfringens as indicator strain) methods of assaying metronidazole have been compared. on dummy samples made with pure metronidazole in pooled human serum, both methods had a coefficient of variation ranging from 5.5 to 9.6 per cent of the mean. the microbiological method slightly underestimated the real amounts, and also had lower values than the chemical proc ... | 1980 | 6279985 |
| [interest in gnotoxenic systems for the study of host-microbial flora of the digestive tract]. | it is necessary to use experimental models in all studies of interactions between a host, its digestive tract microbial flora and the environment because these interactions are complex. the gnotoxenic animal, reared in an isolator as the axenic animal and harbouring a known microbial flora, constitutes either an analytic or mimetic experimental model. the gnotoxenic animal may be considered as an analytic model when used to determine which biotic or abiotic environmental factor of the host-anima ... | 1980 | 6296940 |
| application of serological typing to the investigation of outbreaks of clostridium perfringens food poisoning, 1970-1978. | serological typing was used as an epidemiological tool in the investigation of 524 outbreaks of clostridium perfringens food poisoning in the united kingdom and 37 outbreaks in other countries. five thousand five hundred and fifty-four (77%) of 7245 strains of c. perfringens associated with the 561 outbreaks were typable with the 75 food hygiene laboratory antisera; in 354 (63%) of these outbreaks a specific serotype was established as being responsible for the outbreak. an assessment is made of ... | 1980 | 6300225 |
| acute gastric dilatation in monkeys: a microbiologic study of gastric contents, blood and feed. | twenty-one of 24 simian primates with acute gastric dilatation had clostridium perfringens in their gastric contents. only 2 of 18 normal animals contained this organism in their gastric contents. clostridium perfringens was isolated from monkey biscuits taken from the cages of five affected animals and from five of 11 incoming lots of feed. after these biscuits were fed to normal animals, this organism could be isolated from the gastric contents. there were no other organisms isolated which cou ... | 1980 | 6302390 |
| photo-oxidation of l-glutamate decarboxylase from escherichia coli, sensitized by the coenzyme pyridoxal phosphate and by proflavin. | irradiation of l-glutamate decarboxylase (l-glutamate 1-carboxy-lyase, ec 4.1.1.15) from escherichia coli by visible light absorbed by the intrinsic chromophore, pyridoxal phosphate, caused the selective modification of two methionines per enzyme monomer. the disulfoxide derivative exhibited modified circular dichroism, chromatographic and kinetic properties, suggesting a conformational role for the two methionine residues. irradiation of the enzyme in the presence of proflavin revealed the pres ... | 1980 | 6990995 |
| studies on the sulfite reduction test for clostridia. | peptone-yeast extract (py) medium containing 0.035% ferric ammonium citrate as an indicator, 0.05% sulfite as a substrate, 0.05% cysteine as a reducer and 0.5% glucose was found to be suitable for observing the sulfite reduction test. the effect of added cysteine on the test was suppressed by the addition of glucose. in cultures of bacteria grown for 2 days at 37 c in medium containing the above ingredients, 121 among 132 strains of clostridia, including 86 strains of clostridium perfringens, ga ... | 1980 | 6993870 |
| k-1 antigen content and the presence of an additional sialic acid-containing antigen among bacteremic k-1 escherichia coli: correlation with susceptibility to opsonophagocytosis. | eighty percent of blood culture isolates of escherichia coli k-1 are resistant to in vitro opsonophagocytosis by normal human granulocytes and fresh serum. to determine the basis for susceptibility to phagocytosis in 20% of bacteremic k-1 e. coli, we investigated possible quantitative and qualitative immunochemical differences in the k-1 antigen content among resistant and sensitive isolates. we prepared extracts of blood culture k-1 e. coli by sonication and determined the k-1 polysaccharide co ... | 1980 | 7000702 |
| the clinical significance, taxonomy and special methodological problems of the pathogenic clostridia. | the clinical significance of clostridia is much greater than is generally recognized. the organisms are a major cause of septic abortion, clostridium perfringens being the most important single organism. this species is also the principle agent in food-poisoning. clostridium botulinum is considered to be one of the main causes of the sudden death syndrome in infants. as clostridia are universally distributed in nature and the human body, the isolation of an organism of this group from the human ... | 1980 | 7005089 |
| effective immunization of lambs against enterotoxaemia. | in contrast to adult sheep, 2- to 3-month-old lambs do not respond well to a single injection of clostridium perfringens type d oil adjuvant epsilon toxoid. this unresponsiveness can be overcome, however, by administering 2 injections of oil adjuvant vaccine or one injection of oil adjuvant followed 4 weeks later by an injection of alum-precipitated toxoid. the latter procedure evokes protective antitoxin levels which persist for 8 months, and a booster injection of alum-precipitated toxoid give ... | 1980 | 7231926 |
| [gangrenous cholecystitis without lithiasis. a report on a case diagnosed by simple radiography of the abdomen (author's transl)]. | the authors report a case of gangrenous cholecystitis without lithiasis but with secondary infection of the gallbladder by anaerobic germs. pre-operative diagnosis was possible from simple x-rays of the abdomen which demonstrated an obvious fluid level in the gallbladder, and by parietography. an immediate cholecystectomy was performed. bacteriological examination of the bile confirmed the presence of clostridium perfringens. the main bile duct appeared normal and the bile in the common bile duc ... | 1980 | 7380889 |
| microflora and deconjugation of bile acids in alkaline reflux after partial gastrectomy. | it has been postulated that reflux of bile into the stomach promotes gastric carcinogenesis. bile-stained aspirates from 50 asymptomatic patients, partially gastrectomized more than 10 years earlier, were examined bacteriologically and with regard to conjugated and deconjugated bile acids. endoscopic biopsies showed atrophic gastritis in all patients, cancer in two and severe dysplasia in another two. ph in the reflux aspirates was 7.3 +/- 0.4 (mean +/- standard deviation). bacterial cultures w ... | 1980 | 7406138 |
| clostridium perfringens septicemia following cesarean section. | a clostridium perfringens infection is described in an 18-year-old primigravida following cesarean section at 43 weeks' gestation. despite the ubiquitous distribution of these organisms, such infections are rarely encountered in obstetrics. characteristics of these bacteria and appropriate diagnostic and therapeutic procedures are presented. despite the infrequency of this potentially lethal complication, survival depends on early diagnosis and aggressive treatment. | 1980 | 7422200 |
| [bacteriological and hygienic control of rendering plants (author's transl)]. | procedures for the hygienic inspection of animal rendering plants should include the bacteriological investigation of the meat meal, the bacteriological control of the used methods of disinfection of the contaminated rooms, of the transport vehicles and of the sewage of the contaminated side. meat meal has to be free of clostridium perfringens and salmonellae. clostridium perfringens is an indicator of the sterilizing effect of the rendering procedures. after an effective disinfection of rooms n ... | 1980 | 7424272 |
| effect of sialic acid removal on human low density lipoprotein catabolism in vivo. | this study was undertaken to determine whether sialic acid removal alters the catabolism of low density lipoprotein in humans. human low density lipoproteins labeled in vitro with 125i were incubated in the presence (termed desialylated) or absence (sialylated) of neuraminidase. the treatment with neuraminidase from clostridium perfringens removed 90% of the sialic acid residues which did not change the chemical composition of the lipoproteins. sialylated or desialylated ldl were injected intrav ... | 1980 | 7426100 |
| purification and composition of colonic epithelial mucin. | colonic mucin was purified from homogenized scrapings of rat colonic epithelial cells using gel filtration and ion-exchange chromatography. high molecular weight water-soluble mucin was separated from low molecular weight proteins by gel exclusion chromatography on sepharose 4b, and was further separated into two major mucin fractions and several non-mucin fractions on deae-cellulose. fraction iv, the major mucin, was a sulphated glycoprotein with 62% carbohydrate by weight, and high concentrati ... | 1980 | 7459381 |
| preliminary study on fermentative sub-grouping of enterotoxigenic strain of clostridium perfringens type a in relation to pre-heating temperature. | 1980 | 7461788 | |
| isolation and characterization of a novel monosialosylpentahexosyl ceramide from tay-sachs brain. | a novel monosialoganglioside was isolated from tay-sachs brains. it represented about 0.1% of the total ganglioside mixture. compositional analysis by gas-liquid chromatography indicated that it contained glucose, galactose, n-acetylgalactosamine, n-acetylneuraminic acid, and long chain base in the molar ratio of 1:2:2:1:1. the ganglioside was found to be resistant to neuraminidase (clostridium perfringens), beta-hexosaminidase (jack bean), and beta-galactosidase. however, it could be degraded b ... | 1981 | 7451432 |
| [haemolysis as initial sign of clostridium perfringens septicaemia in newborn (author's transl)]. | cases of clostridium perfringens, septicaemia are subject to a very gloomy prognosis in adults. this applies likewise to the rare cases reported in paediatric literature. the present report describes the fatal case of a newborn with clostridium perfringens septicaemia. the principal sign was a severe haemolysis which failed to respond to blood exchange transfusions and antibiotic treatment. the characteristic changes of colour of the skin usually observed in septicaemia of newborn had probably b ... | 1981 | 7289436 |
| intrasplenic abscess: two case reports and review of the literature. | the occurrence of splenic abscess, sickle cell trait, and drug addiction is described in two patients. the coincidence of sickle cell disease and drug addiction in the possible etiology of splenic abscess should be noted. the first patient had a splenic abscess in the inferior pole of the spleen with signs and symptoms of left upper quadrant peritoneal irritation. the second patient had an intrasplenic abscess in the superior pole with pleuritic type chest pain and large left pleural effusion. i ... | 1981 | 7305137 |
| analysis of membrane structure in the transitional epithelium of rat urinary bladder. 3. localization of cholesterol using filipin and digitonin. | 1981 | 7310938 | |
| isolation and preliminary characterization of novel polysialogangliosides from embryonic chick brain. | preparative thin-layer chromatography was used to isolate from embryonic chick brains four different polysialogangliosides which moved below gt1b on tlc plates. these possessed molar sialic acid-to-sphingosine ratios of 4:1, 5:1, 6:1, and 7:1, respectively. mild neuraminidase (clostridium perfringens) treatment of the three most highly sialylated fractions gave gt1b and gd1b transiently, but the only accumulating ganglioside was gm1. all three polysialogangliosides were also found in elasmobranc ... | 1981 | 7320736 |
| comparative in vitro activity of new beta-lactam antibiotics against anaerobic bacteria. | several new beta-lactam antimicrobial agents have been introduced in the last few years. in this investigation, the in vitro activities of several recently introduced cephalosporins (cefoperazone, cefotaxime, ceftazidime, and ceftizoxime), moxalactam, and n-formimidoyl thienamycin were compared with those of cefoxitin, clindamycin, and metronidazole against 203 strains of anaerobic bacteria. at achievable serum levels, all of the antimicrobial agents were active against essentially 100% of the s ... | 1981 | 7325628 |
| the release of n-acetyl- and n-glycolloyl-neuraminic acid from soluble complex carbohydrates and erythrocytes by bacterial, viral and mammalian sialidases. | a series of substrates, sialyl(2 leads to 6)galnac and ganglioside gm3, containing either n-acetylneuraminic acid (acneu) or n-glycolloylneuraminic acid (gcneu), has been prepared. the trisaccharide gcneu(2 leads to 3)lactose was preapred by ozonolysis of gcneu-gm3, and the disaccharides acneu(2 leads to 6)galnac and gcneu(2 leads to 6)galnac were isolated from bovine submandibular-gland mucin by alkali elimination. sialidases from newcastle-disease virus, fowl-plague virus, influenza virus a2, ... | 1981 | 7325957 |
| [effect of the sporotoxin of clostridium perfringens (welchii) on isolated intestine of the rabbit]. | 1981 | 7348320 | |
| membrane-damaging action of alveolysin from bacillus alvei. | we investigated membrane damage to human diploid, embryonic lung fibroblasts caused by highly purified alveolysin by measuring leakage of cytoplasmic markers and uptake of various metabolites, and we observed a leakage pattern typical of sh-activated cytolysins. however, the mode of membrane interaction resembled the mode of membrane interaction of theta-toxin from clostridium perfringens rather than that of streptolysin o in the following respects: the activity on fibroblast membranes was high ... | 1981 | 6894743 |
| metronidazole: in vitro activity, pharmacology and efficacy in anaerobic bacterial infections. | metronidazole is a 5-nitroimidazole that has selective activity against anaerobic microorganisms, including bacteria and protozoa. intravenous metronidazole has recently been approved by the u.s. food and drug administration for the treatment of serious anaerobic bacterial infections. it is usually bactericidal at low concentrations, and its spectrum of activity encompasses almost all anaerobic bacteria and some capnophilic organisms. anaerobic bacteria known to be resistant to metronidazole inc ... | 1981 | 6927601 |
| nitroimidazoles: in vitro activity and efficacy in anaerobic infections. | nitroimidazoles, including metronidazole, tinidazole and ornidazole, are low molecular weight antimicrobial compounds with excellent activity against anaerobic microorganisms. these compounds are usually bactericidal at low concentrations and their spectrum of activity encompasses almost all the anaerobic bacteria and some capnophylic organisms. the few anaerobic bacteria known to be resistant to the nitroimidazoles include occasional anaerobic cocci, some nonsporing gram positive bacilli and pr ... | 1981 | 6941456 |
| [anaerobic bacteria in clinical specimens of a hospital laboratory (author's transl)]. | over a 14-month period 261 clinical specimens were cultured anaerobically, this figure representing about 3% of the total specimens submitted to the microbiological laboratory. not accepted for anaerobic culture were stools, sputa, urines, vaginal and urethral swabs and swabs from superficial skin lesions. all materials were sent to the laboratory in an anaerobic transport medium and processed according to the recommendations of the american society for microbiology and the center for disease co ... | 1981 | 7020261 |
| isolation and structural characterization of human lymphocyte and neutrophil gangliosides. | gangliosides were isolated from purified preparations of human peripheral blood lymphocytes and neutrophils. structural analyses and comparisons were performed by direct probe mass spectrometry and by degradation studies with the following enzymes: escherichia freundii endo-beta-galactosidase; clostridium perfringens and arthrobacter ureafaciens neuraminidase; and jack bean beta-n-acetylhexosaminidase and beta-galactosidase. this combination of techniques allowed us to obtain carbohydrate compos ... | 1981 | 6780555 |
| immunodiffusion method for detection of type a clostridium botulinum. | a simple gel immunodiffusion agar procedure was developed for detecting toxigenic strains of clostridium botulinum type a. the method consisted of overlaying colonies grown on thin-layer tryptone-peptone-glucose-yeast extract agar with gel diffusion agar containing desired levels of c. botulinum type a antitoxin. concentric precipitin zones formed around colonies of c. botulinum type a. strains of c. botulinum type a were detected by this procedure. however, c. botulinum type b reacted to a less ... | 1981 | 6797350 |
| regulation of fatty acid composition of cl. perfringens type a cells by hydrogen and hydroxyl ions. | the composition of the fatty acid profiles of cl. perfringens type a, grown on media with initial ph values from 5.5 to 9.0, was determined by the method of gas-liquid chromatography. the fatty acid profiles are stabilized in 18- to 24-h cultures. hydrogen ions stimulate the synthesis of cyclopropane carboxylic fatty acids and "desaturase" activity; hydroxyl ions inhibit these processes. the content of saturated fatty acids in the bacteria is regulated by the initial acid-alkaline conditions of ... | 1981 | 6284259 |
| acute gastric dilation and rupture in macaca arctoides associated with clostridium perfringens. | a clinical case of gastric dilation and rupture is described in an adult male macaca arctoides. clostridium perfringens was isolated from the heart blood and liver. data collected from our macaque colony for 13 years indicated a mortality rate of 3.5% due to acute dilation. | 1981 | 6283084 |
| [study of the microbial multiplication in the muscle just after slaughter (author's transl)]. | the bactericidal or bacteriostatic activity of the serum, the muscle and the rabbit meat juice was followed after slaughter on animals vaccinated or not against e. coli, p. putida, cl. perfringens. rabbit serum is bactericidal against gram negative bacteria, p. putida and e. coli. on the contrary it is without effect on cl. perfringens. muscle has no inhibitory effect against e. coli and cl. perfringens on animals vaccinated or not. p. putida is inhibited or partially eliminated from rabbit musc ... | 1981 | 6280415 |
| [method of determining the biochemical behavior of clostridia]. | a fast and readily applicable method is suggested for the determination of the biochemical reactions of the spore producing anaerobic organisms. a pepton coloid medium with the addition of 0.15 per cent agar-agar was used as a basic nutrient medium. the cultivation of bacteria did not require any special conditions. reading of the results was carried out with the addition of 0.1 per cent phenol red after 48 hours of incubation of the cultures. this method showed a number of advantages over the m ... | 1981 | 6283724 |
| pediatric surgical patients with severe anaerobic infection: report of 16 t-antigen positive cases and possible hazards of blood transfusion. | red blood cells become polyagglutinable when the normally latent t-antigens of the red blood cell membrane are exposed. unmasking of t-antigens results from removal of n-acetyl-neuraminic acid by neuraminidase, an enzyme commonly produced by a variety of bacteria. red blood cells altered in this way are said to be t-activated. t-activated red blood cells can be agglutinated by anti-t, an antibody normally present in human serum, so that severe transfusion reactions may occur and have occurred, i ... | 1981 | 6279816 |
| extraction of spore-lytic enzyme from clostridium perfringens spores. | various chemical reagents known to extract spore coat protein were used to extract spore-lytic enzyme (sle) from intact and germinated spores of clostridium perfringens. of the reagents tested, 7.2 m-urea plus 10% (v/v) mercaptoethanol, ph 2.85, solubilized the most sle activity per mg spores. the quantity of sle extracted was dependent on the initial ph of the reagent, with a maximum between ph 2.7 and 3.0. germinated spores yielded more sle than non-germinated spores upon urea/mercaptoethanol ... | 1981 | 6278055 |
| the camp test performed by using staphylococcus aureus sphingomyelinase (beta-haemolysin) and clostridium perfringens lecithinase. | the camp test was performed by employing staphylococcus aureus beta-haemolysin (sphingomyelinase) and clostridium perfringens alpha-toxin (lecithinase) for identification of group b streptococci on blood agar, using blood from different origin. partial purification of cl perfringens alpha-toxin was carried out by means of sheep erythrocytes. with the toxin preparations described positive camp reaction was obtained only on sheep blood agar. false positives with regard to group a streptococci coul ... | 1981 | 6278370 |
| resistance to chloramphenicol and metronidazole in anaerobic bacteria. | 1981 | 6279556 | |
| bacteriologic examination of equine fecal flora as a diagnostic tool for equine intestinal clostridiosis. | the fecal flora of 56 clinically healthy and 23 sick horses were examined bacteriologically for counts of clostridium perfringens, molds, coliforms, alpha- and beta-hemolytic streptococci, and microbes belonging to genus bacillus, as well as for the presence of salmonella spp. of the healthy horses, 85.7% had a c perfringens count less than 10(1) colony-forming units/g of feces. of the healthy horses, lowest counts were found in race-horses. of the sick horses, equine intestinal clostridiosis wa ... | 1981 | 6280523 |
| the detection of the factor lowering the immunogenicity of toxoid of clostridium perfringens type a. | 1981 | 6280638 | |
| acute gastric dilatation in common marmosets (callithrix jacchus). | 1981 | 6281555 | |
| [gas phlegmon of the neck and chest (author's transl)]. | 1981 | 6281714 | |
| [clostridium perfringens in meat products in chile: contamination level and enteric toxicity (author's transl)]. | 1981 | 6281871 | |
| characterization of the major apolipoproteins secreted by two human hepatoma cell lines. | two newly described human hepatoma derived cell lines, hep g2 and hep 3b [knowles, b. b., howe, c. c., & aden, d. p. (1980) science (washington, d.c.) 209, 497-499], synthesize and secrete into the culture medium most of the major plasma apoproteins (apoa-i, apoa-ii, apob, apoc-ii, apoc-iii, and apoe). the synthesized apoproteins were identified by direct two-dimensional gel analysis of the culture medium or by two-dimensional analysis following purification of the apoproteins by ultracentrifuga ... | 1981 | 6274388 |
| an outbreak of clostridium perfringens hobbs type 21 food poisoning. | 1981 | 6275630 | |
| dispersal and fate of coliphages in the river saar. | the dispersal of coliphages was analysed quantitatively in longitudinal profiles of the partially canalized river saar. the numbers of coliphages ranged between 0 and 2380 pfu (plaque-forming units)/ml in the water and between 0 and 2550 pfu/g in the sediments, depending on the degree of fecal pollution. in canalized parts the phages were found mainly in the sediments, whereas in non-canalized parts they prevailed in the water phase (fig. 3). determinations performed at municipal sewage water ou ... | 1981 | 6275634 |
| [immunogenic properties of clostridium perfringens type a anatheta-hemolysin]. | guinea-pigs were immunized with anatheta-hemolysin preparations adsorbed on aluminum hydroxide, as well as with the mixture of anatheta-hemolysin and type a cl. perfringens toxoid, purified and concentrated. anatheta hemolysin preparations were obtained with the use of homogeneous theta hemolysin, as well theta hemolysin of various purification degrees. as a result, antatheta hemolytic guinea-pig sera capable of neutralizing 2,000-8,000 hu of theta-hemolysin were obtained. tests made to establis ... | 1981 | 6275640 |
| penicillin-binding proteins in clostridium perfringens. | the penicillin-binding proteins (pbps) of clostridium perfringens were studied. six pbps ranging in molecular weight from approximately 42,000 to 100,000 were detected in the cytoplasmic membrane. the relative affinities of the pbps for 16 beta-lactam antibiotics were determined. most of the drug saturated pbp 3 and 4 at concentrations equal to their minimal inhibitory concentrations, suggesting that these pbps are the killing targets for beta-lactams. mecillinam showed unique properties; it had ... | 1981 | 6275787 |
| [incidence and survival of clostridium perfringens in soil (author's transl)]. | 1981 | 6276587 | |
| [differentiation of pathogenic clostridia from material collected in the field and of reference strains by means of biostatistical analysis of the quantitatively recorded metabolic fatty acid patterns of the agents (author's transl)]. | 34 reference and 9 exotic field strains of clostridia were analyzed by means of gas liquid chromatography (glc) on their patterns of metabolic short chain fatty acids. to increase the number of criteria for the differentiation specimens were grown on pepton yeast medium with addition of 13 different carbohydrates and alcohols. data of sequence and mean square space of glc-peaks were stored in a data bank. with the spss discriminance analytical system the field strains were compared with the refe ... | 1981 | 6277102 |
| [production of anti-enterotoxin antiserum to clostridium perfringens type a toxin]. | 1981 | 6277108 | |
| toxigenic clostridium perfringens from a parvovirus-infected dog. | a strain of clostridium perfringens, type a, has been isolated from the intestine of a dog which died from parvovirus infection. this clostridium strain produces a toxin which can be detected by counterimmunoelectrophoresis, using c. difficile antitoxin, and produces cytotoxicity in wi-38 cell culture. cytopathology can be blocked by c. difficile antitoxin. its role in canine parvovirus infection is unknown. | 1981 | 6277989 |
| endogenous radiolabeling of enterotoxin from clostridium perfringens type a on a defined medium. | four enterotoxin-positive strains of clostridium perfringens were tested for sporulation and enterotoxin production on defined media. the medium described by sacks and thompson (appl. environ. microbiol. 35:405-409, 1978) gave the highest enterotoxin production and was selected for the production of endogenously labeled enterotoxin. the specific radioactivity of the enterotoxin was 16,000 dpm/microgram when the tritiated amino acids were added to the growth medium just before the inoculum. addit ... | 1981 | 6279031 |
| [foodborne intoxications by clostridium perfringens type a in chicken salad (author's transl)]. | 1981 | 6279380 | |
| clostridium perfringens in faeces of healthy persons and diarrhoeal patients. | 1981 | 6279492 | |
| [fate of clostridium perfringens in intestine of guinea pigs after oral ingestion (author's transl)]. | 1981 | 6285030 | |
| macrolide-lincosamide-streptogramin resistance patterns in clostridium perfringens from animals. | different patterns of resistance against commonly used macrolide, lincosamide, and streptogramin antibiotics were found in clostridium perfringens of animal origin. the patterns were designated as (i) macrolide-lincosamide-streptogramin group b generalized resistance, (ii) macrolide-lincosamide generalized resistance, (iii) macrolide-lincosamide inducible resistance, and (iv) macrolide-lincosamide-streptogramin low-level generalized resistance. the strains of the fourth pattern were able to inac ... | 1981 | 6289728 |
| [epidemiology of nosocomial clostridial myonecrosis (gas gangrene) (author's transl)]. | the occurence of clostridium perfringens in the environment of different hospital wards and on the skin and in the faeces of in-hospital patients was examined. the author holds the view that most cases of manifest gas gangrene are caused by autogenous infection and not coming from outside. | 1981 | 6269329 |
| [hygienic problems at the winning of peloids (peats and sludges of lakes) for balneological therapy (author's transl)]. | in austria there exist no regulations for the quality of peats and sludges of lakes for the external usage in balneotherapy. the results of our examinations showed that peats on the whole (fig. 1) have much better bacteriological results than sludges of shallow, eutrophic lakes (fig. 2). typical intestinal germs appeared only in one of the five examined peats during a rain period down to 0.6 m beneath the surface. three of the peats showed satisfying local and bacteriological conditions, one of ... | 1981 | 6269330 |
| small unilamellar vesicles containing glycophorin a. chemical characterization and proton nuclear magnetic resonance studies. | glycophorin a, a major glycoprotein of the red blood cell, is reconstituted in small lipid vesicles (250-300 a in diameter) by using cholate detergent solubilization followed by rapid removal of cholate on a molecular sieve column. the extent of glycophorin incorporation is found to be critically dependent on the amount of cholate used, with higher amounts yielding vesicles with higher percentages of glycophorin. vesicles with as much as 1 molecule of protein per 20 molecules of lipid can be pre ... | 1981 | 6269587 |
| a basic isozyme of yeast glyceraldehyde-3-phosphate dehydrogenase with nucleic acid helix-destabilizing activity. | a nucleic acid helix-destabilizing protein has been purified from saccharomyces cerevisiae using affinity chromatographic techniques. crude protein extracts at low ionic strength (approx. 0.05 m) were applied sequentially to tandem columns of native dna-cellulose, aminophenyl-phosphoryl-ump-agarose, poly(i . c)-agarose, poly(u)-cellulose and denatured dna-cellulose. the 2 m nacl eluant of the poly(u)-cellulose column was dialyzed to low ionic strength and recycled through native dna-cellulose, p ... | 1981 | 6269621 |
| clostridium perfringens type c enteritis in a quebec swine herd. | 1981 | 6269716 | |
| [pyarthron with clostridium perfringens after injections of a steroid preparation]. | 1981 | 6270864 | |
| introduction and reisolation of selected gram-positive bacteria from fermented edible wastes. | a fermentation process using lactobacillus acidophilus added to edible food wastes was evaluated for its bactericidal action on selected gram-positive organisms. the lactobacillus fermentation converts food wastes into an animal feed ingredient. in this study, 5 gram-positive bacteria of zoonotic importance were individually tested. these organisms were: group e streptococcus, erysipelothrix rhusiopathiae, clostridium perfringens, corynebacterium pseudotuberculosis, and listeria monocytogenes. f ... | 1981 | 6271028 |
| transformation of bile acids by clostridium perfringens. | thirty-five strains of clostridium perfringens were examined for their ability to transform bile acids, both in growing cultures and by washed whole cells. all of the strains oxidized the 3 alpha-hydroxy group to an oxo group, and all except three converted the same alpha-hydroxy group into a beta-configuration. the oxidative 3 alpha-dehydrogenation was barely detectable under anaerobic cultural conditions but was clearly demonstrated in an aerated system using washed whole cells, with a ph opti ... | 1981 | 6271056 |
| food borne disease. | 1981 | 6271107 | |
| [factors modulating production of antibodies against clostridium perfringens in mammals]. | immunogenic properties of clostridium perfringens type a toxoid preparations obtained by different methods are described. as regards immunogenicity for guinea-pigs and man, toxoid obtained by the detoxification of preliminarily purified alpha-toxin (experimental toxoid) compares favourably with preparations obtained by the detoxification of alpha-toxin in a culture fluid. it was shown in experiments on guinea pigs that immunogenicity of experimental toxoid rises with the increase in the degree o ... | 1981 | 6271303 |
| stimulation of clostridium perfringens enterotoxin formation by caffeine and theobromine. | in the presence of 100 micrograms of caffeine per ml or 200 micrograms of theobromine per ml, sporulation of clostridium perfringens nctc 8679 rose from less than 1 to 80 or 85%. enterotoxin concentration increased from undetectable levels to 450 micrograms/mg of cell extract protein. heat-resistant spore levels increased from less than 1,000 to between 1 x 10(7) and 2 x 10(7)/ml. these effects were partially reversible by the addition of adenosine or thymidine. in the case of nctc 8238, caffein ... | 1981 | 6271685 |
| a study of rapid and simplified confirmatory tests for clostridium perfringens. | 1981 | 6271721 | |
| [tests for the presence of bacteriophages in live virus vaccine]. | 1981 | 6272037 | |
| further studies on complementation between mutants of clostridium perfringens. | 1. mutants devoid of lambda- and kappa-toxin and hemagglutinin (ha), respectively, were isolated from cl. perfringens pb6k. the lambda- and ha- mutants could be classified into a and b groups by complementation but the kappa- mutants were all of the a group. 2. all b group mutants isolated, irrespective of the marker used for isolation, were pleiotropically negative or leaky with respect to theta-, lambda- and kappa-toxin and ha production. 3. lambda-toxin produced by complementation was proved ... | 1981 | 6272687 |