Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| selectivity and specificity of a chromogenic medium for detecting vibrio parahaemolyticust. | the thiosulfate-citrate-bile salts-sucrose agar (tcbs) used in the most-probable-number method for detecting vibrio parahaemolyticus cannot differentiate growth of v. parahaemolyticus from vibrio vulnificus or vibrio mimicus. this study examined the selectivity and specificity of bio-chrome vibrio medium (bcvm), a chromogenic medium that detects v. parahaemolyticus on the basis of the formation of distinct purple colonies on the medium. a panel consisting of 221 strains of bacteria, including 17 ... | 2005 | 16013386 |
| thermostable direct hemolysin of vibrio parahaemolyticus is a bacterial reversible amyloid toxin. | thermostable direct hemolysin (tdh), a major virulence factor of vibrio parahaemolyticus, is detoxified by heating at approximately 60-70 degrees c but is reactivated by additional heating above 80 degrees c. this paradoxical phenomenon, known as the arrhenius effect, has remained unexplained for approximately 100 years. we now demonstrate that the effect is related to structural changes in the protein that produce fibrils. the native tdh (tdhn) is transformed into nontoxic fibrils rich in beta- ... | 2005 | 16026154 |
| pandemic vibrio parahaemolyticus o3:k6 spread, france. | 2005 | 16032794 | |
| molecular typing of vibrio parahaemolyticus strains isolated from the philippines by pcr-based methods. | the main aim of the present study was to use three pcr-based techniques for the analysis of genetic variability among vibrio parahaemolyticus strains isolated from the philippines. | 2005 | 16033470 |
| investigation of seven vibrio virulence genes among vibrio alginolyticus and vibrio parahaemolyticus strains from the coastal mariculture systems in guangdong, china. | to investigate the distribution of the virulence of two vibrio species among different strains obtained from the mariculture systems on the coast of guangdong in china and the correlation between the virulence strains and the virulence genes among vibrio alginolyticus. | 2005 | 16033522 |
| white spot syndrome virus isolates of tiger shrimp penaeus monodon (fabricious) in india are similar to exotic isolates as revealed by polymerase chain reaction and electron microscopy. | microbiological analysis of samples collected from cases of white spot disease outbreaks in cultured shrimp in different farms located in three regions along east coast of india viz. chidambram (tamil nadu), nellore (andhra pradesh) and balasore (orissa), revealed presence of vibrio alginolyticus, vibrio parahaemolyticus, and aeromonas spp. but experimental infection trials in penaeus monodon with these isolates did not induce any acute mortality or formation of white spots on carapace. infectio ... | 2005 | 16053274 |
| microarray analysis identifies apoptosis regulatory gene expression in hct116 cells infected with thermostable direct hemolysin-deletion mutant of vibrio parahaemolyticus. | the thermostable direct hemolysin (tdh) is considered as a major virulence factor of vibrio parahaemolyticus. we observed this potential in several human cancer cell lines by using the tdh-producing wild-type (rimd2210633) as well as tdh-deletion mutant of v. parahaemolyticus and found that the deletion of tdh did not affect cytotoxicity to any of the cell lines tested. dna fragmentation and annexin v staining showed that both wild-type and tdh-mutant trigger apoptosis in these cells. to underst ... | 2005 | 16061205 |
| rapid phenotypic characterization of vibrio isolates by pyrolysis metastable atom bombardment mass spectrometry. | pyrolysis mass spectrometry was investigated for rapid characterization of food-borne bacterial pathogens. nine isolates of vibrio parahaemolyticus and one isolate each of vibrio fluvialis, vibrio hollisae, and vibrio vulnificus were analyzed. pyrolysis mass spectra, generated via an alternative ionization method, metastable atom bombardment, were subject to principal component-discriminant analysis. the spectral patterns were used to distinguish vibrio isolates differing in species, serotype an ... | 2005 | 16096691 |
| characterization of new o3:k6 strains and phylogenetically related strains of vibrio parahaemolyticus isolated in taiwan and other countries. | we analysed the genetic divergence in the pandemic new o3:k6 and phylogenetically related (new o3:k6-like) strains and compare these two groups in terms of virulence and other biological traits. | 2005 | 15715859 |
| identification of essential amino acid residues of the norm na+/multidrug antiporter in vibrio parahaemolyticus. | norm is a member of the multidrug and toxic compound extrusion (mate) family and functions as a na+/multidrug antiporter in vibrio parahaemolyticus, although the underlying mechanism of the na+/multidrug antiport is unknown. acidic amino acid residues asp32, glu251, and asp367 in the transmembrane region of norm are conserved in one of the clusters of the mate family. in this study, we investigated the role(s) of acidic amino acid residues asp32, glu251, and asp367 in the transmembrane region of ... | 2005 | 15716425 |
| the promoter region rather than its downstream inverted repeat sequence is responsible for low-level transcription of the thermostable direct hemolysin-related hemolysin (trh) gene of vibrio parahaemolyticus. | we determined the transcriptional start site of the thermostable direct hemolysin-related hemolysin gene (trh) of vibrio parahaemolyticus by using a pcr-based method and identified the promoter. mutagenic analysis indicated that the promoter-bearing region rather than its downstream inverted repeat sequence was responsible for the low-revel of trh transcription. | 2005 | 15716457 |
| comparison of kinetic models to describe high pressure and gamma irradiation used to inactivate vibrio vulnificus and vibrio parahaemolyticus prepared in buffer solution and in whole oysters. | comparisons of different models in inactivation kinetics were conducted on data obtained from high-pressure and gamma-irradiation processing. vibrio vulnificus (mo-624) and vibrio parahaemolyticus (o3:k6 tx-2103) suspended in phosphate-buffered saline (ph 7.4, 10(7) cfu/ml) were exposed to pressures from 207 to 379 mpa for 1 to 20 min. inoculated whole oysters (106 cfu/g) were exposed to pressure from 276 to 379 mpa for 1 to 15 min. pure cultures and inoculated oysters (10(6) cfu/g) also were ir ... | 2005 | 15726971 |
| vibrionaceae as a possible source of qnr-like quinolone resistance determinants. | to gain insight into the functionality of qnr-like genes of several bacterial species of vibrionaceae that may encode quinolone resistance determinants. | 2005 | 16227349 |
| [faster detection of vibrio parahaemolyticus in foods by fq-pcr technique]. | to establish a rapid and accurate qualitative and quantitative method to detect vibrio parahaemolyticus in food. | 2005 | 16229276 |
| a novel, conserved cluster of genes promotes symbiotic colonization and sigma-dependent biofilm formation by vibrio fischeri. | vibrio fischeri is the exclusive symbiont residing in the light organ of the squid euprymna scolopes. to understand the genetic requirements for this association, we searched a library of v. fischeri transposon insertion mutants for those that failed to colonize e. scolopes. we identified four mutants that exhibited severe defects in initiating colonization. sequence analysis revealed that the strains contained insertions in four different members of a cluster of 21 genes oriented in the same di ... | 2005 | 16102015 |
| pandemic vibrio parahaemolyticus o3:k6, europe. | 2005 | 16110585 | |
| vpah, a gene encoding a novel histone-like nucleoid structure-like protein that was possibly horizontally acquired, regulates the biogenesis of lateral flagella in trh-positive vibrio parahaemolyticus th3996. | a histone-like nucleoid structure (h-ns) is a major component of the bacterial nucleoid and plays a crucial role in the global gene regulation of enteric bacteria. here, we cloned and characterized the gene for the h-ns-like protein vpah in vibrio parahaemolyticus. vpah encodes a protein of 134 amino acids that shows approximately 55%, 54%, and 41% identities with vich in vibrio cholerae, h-ns in v. parahaemolyticus, and h-ns in escherichia coli, respectively. the vpah gene was found in only trh ... | 2005 | 16113292 |
| physiological roles of three na+/h+ antiporters in the halophilic bacterium vibrio parahaemolyticus. | vibrio parahaemolyticus mutants lacking three na+/h+ antiporters (nhaa, nhab, nhad) were constructed. the deltanhaa strains showed significantly higher sensitivity to licl regarding their growth compared to the parental strain. the deltanhaa and deltanhab strains exhibited higher sensitivities to licl. the mutant xacabd lacking all of the three antiporters could not grow in the presence of 500 mm licl at ph 7.0, or 50 mm at ph 8.5. the xacabd mutant was also sensitive to 1.0 m nacl at ph 8.5. th ... | 2005 | 16113500 |
| an estuarine neritid gastropod, clithon corona, a potential reservoir of thermostable direct hemolysin-producing vibrio parahaemolyticus. | an estuarine neritid gastropod, clithon corona, maintained in uv-irradiated recirculating artificial seawater with a salinity of 15 per mil (%o) was found to retain thermostable direct hemolysin (tdh)-producing vibrio parahaemolyticus in the gut at significantly higher levels than tdh-non-producing one for at least 14 days. another estuarine neritid gastropod, c. sowerbianus, was not able to support the preferential survival of tdh-producing organisms. this evidence suggests that, if tdh-produci ... | 2005 | 16141674 |
| a persistent, productive, and seasonally dynamic vibriophage population within pacific oysters (crassostrea gigas). | in an effort to understand the relationship between vibrio and vibriophage populations, abundances of vibrio spp. and viruses infecting vibrio parahaemolyticus (vpvs) were monitored for a year in pacific oysters and water collected from ladysmith harbor, british columbia, canada. bacterial abundances were highly seasonal, whereas high titers of vpvs (0.5 x 10(4) to 11 x 10(4) viruses cm(-3)) occurred year round in oysters, even when v. parahaemolyticus was undetectable (< 3 cells cm(-3)). viruse ... | 2005 | 16151121 |
| a novel bacteriocin-like substance (blis) from a pathogenic strain of vibrio harveyi. | inter-strain and inter-species inhibition mediated by a bacteriocin-like inhibitory substance (blis) from a pathogenic vibrio harveyi strain vib 571 was demonstrated against four isolates of the same species, and one culture each of a vibrio sp., vibrio fischeri, vibrio gazogenes and vibrio parahaemolyticus. the crude blis, which was obtained by ammonium-sulphate precipitation of the cell-free supernatant of a 72 h broth culture of strain vib 571, was inactivated by lipase, proteinase k, pepsin, ... | 2005 | 16151215 |
| evaluation of toxicity of capsaicin and zosteric acid and their potential application as antifoulants. | the toxicity of two natural product antifoulants, capsaicin and zosteric acid, was evaluated using the microtox assay and a static toxicity test. the ec50 values obtained from the microtox assay for capsaicin and zosteric acid were 11.75 +/- 1.02 and 442 +/- 100 mg/l, respectively. the static toxicity test, conducted with freshwater organisms, yielded capsaicin ec50 values of 5.5 +/- 0.5 and 23 +/- 2.0 mg/l for p. putida and lake erie bacteria, respectively. zosteric acid ec50 values were 167 +/ ... | 2005 | 16161071 |
| outbreak of vibrio parahaemolyticus gastroenteritis associated with alaskan oysters. | vibrio parahaemolyticus, the leading cause of seafood-associated gastroenteritis in the united states, typically is associated with the consumption of raw oysters gathered from warm-water estuaries. we describe a recognized outbreak of v. parahaemolyticus infection associated with the consumption of seafood from alaska. | 2005 | 16207848 |
| detection of vibrio parahaemolyticus in cockle (anadara granosa) by pcr. | this study aimed to determine the occurrence of vibrio parahaemolyticus in cockles (anadara granosa) at a harvesting area and to detect the presence of virulent strains carrying the thermostable direct hemolysin (tdh) and tdh-related hemolysin genes (trh) using pcr. of 100 samples, 62 were positive for the presence of v. parahaemolyticus with an mpn (most probable number) value greater than 3.0 (>1100 mpn per g). the pcr analysis revealed 2 samples to be positive for the tdh gene and 11 to be po ... | 2005 | 16216442 |
| detection and identification of intestinal pathogenic bacteria by hybridization to oligonucleotide microarrays. | to detect the common intestinal pathogenic bacteria quickly and accurately. | 2005 | 16437687 |
| [acquisition and evolution of virulence gene systems in pathogenic bacteria]. | 2005 | 16411452 | |
| prevalence and effectiveness of an education program on intestinal pathogens in food handlers. | to assess the prevalence of intestinal pathogens and the effectiveness of an education program in food handlers in a tertiary care hospital. | 2005 | 16850637 |
| cholera epidemic in baghdad during 1999: clinical and bacteriological profile of hospitalized cases. | an epidemic of cholera in iraq was anticipated for the year 1999 and a plan of notification and treatment of cases of diarrhoea was made. this paper documents the clinical and bacteriological profile of cholera cases admitted to the 6 hospitals in baghdad during the epidemic. the number of stool culture-positive cases was 874. the peak incidence of cases (june 1999) was earlier than previous epidemics with no cases registered in november/december 1999. all age groups were affected and the case f ... | 2005 | 16532666 |
| inactivation of vibrio parahaemolyticus and vibrio vulnificus in phosphate-buffered saline and in inoculated whole oysters by high-pressure processing. | inactivation studies for vibrio parahaemolyticus tx-2103 (serotype o3:k6) and vibrio vulnificus mo-624 (clinical isolate) were conducted in phosphate-buffered saline (pbs) and in inoculated oysters under high-pressure processing conditions. v. parahaemolyticus was more resistant than v. vulnificus in pbs at all pressures and times. a 6-log reduction of v. parahaemolyticus and v. vulnificus in pbs at 241 mpa required 11 and 5 min, respectively, which included a 3-min pressure come-up time. a 4.5- ... | 2006 | 16541691 |
| three new regulators of swarming in vibrio parahaemolyticus. | movement on surfaces, or swarming motility, is effectively mediated by the lateral flagellar (laf) system in vibrio parahaemolyticus. expression of laf is induced by conditions inhibiting rotation of the polar flagellum, which is used for swimming in liquid. however, not all v. parahaemolyticus isolates swarm proficiently. the organism undergoes phase variation between opaque (op) and translucent (tr) cell types. the op cell produces copious capsular polysaccharide and swarms poorly, whereas the ... | 2006 | 16547050 |
| involvement of radical species in inactivation of vibrio parahaemolyticus in saline solutions by direct-current electric treatment. | the effect of pulsed low-direct-current (dc) electric treatment on the viability of vibrio parahaemolyticus in artificial seawater and 3.0% (w/v) nacl solution was studied as a function of available chlorine (ac) concentration. the amount of ac generated during the dc electric treatment increased in proportion to the amount of passed dc. the survival fraction of v. parahaemolyticus cells decreased depending on ac concentration. when the generated ac components were completely reduced in the pres ... | 2006 | 17189175 |
| characterization of a vibrio alginolyticus strain, isolated from alaskan oysters, carrying a hemolysin gene similar to the thermostable direct hemolysin-related hemolysin gene (trh) of vibrio parahaemolyticus. | a vibrio strain isolated from alaskan oysters and classified by its biochemical characteristics as vibrio alginolyticus possessed a thermostable direct hemolysin-related hemolysin (trh) gene previously reported only in vibrio parahaemolyticus. this trh-like gene was cloned and sequenced and was 98% identical to the trh2 gene of v. parahaemolyticus. this gene seems to be functional since it was transcriptionally active in early-stationary-phase growing cells. to our knowledge, this is the first r ... | 2006 | 17056701 |
| antibacterial spectrum of plant polyphenols and extracts depending upon hydroxyphenyl structure. | the relationship between the structure and antibacterial activity of 22 polyphenols was analyzed by using minimum inhibitory concentration (mic) as a criterion against 26 species of bacteria which can grow in mueller-hinton medium. there was no clear correlation between gram-staining and bacterial susceptibility to polyphenols, and the extent of the susceptibility was approximately dependent on the species of bacteria. in the same gram-negative bacteria, the antibacterial activity of the polyphe ... | 2006 | 17077519 |
| high amount of dinophysistoxin-3 in mytilus chilensis collected in seno de reloncaví, chile, during massive human intoxication associated with outbreak of vibrio parahaemolyticus. | this study describes the detection of high amount of 7-o-acyl-derivative dinophysistoxin-1 (dinophysistoxin-3) in filter bivalves collected on february 2005 in the seno de reloncaví, puerto montt city, southern chile, in the same period of time where an intoxication episode was associated with the presence of vibrio parahaemolyticus in shellfish. the diarrhetic shellfish poisoning (dsp) mouse bioassay of mussel extract samples, performed as described for regulatory testing, were negative to dsp ... | 2006 | 17077585 |
| efficacy of electrolysed oxidizing water in inactivating vibrio parahaemolyticus on kitchen cutting boards and food contact surfaces. | to determine the efficacy of electrolysed oxidizing (eo) water in inactivating vibrio parahaemolyticus on kitchen cutting boards and food contact surfaces. | 2006 | 17083714 |
| [development of a predictive program for microbial growth under various temperature conditions]. | a predictive program for microbial growth under various temperature conditions was developed with a mathematical model. the model was a new logistic model recently developed by us. the program predicts escherichia coli growth in broth, staphylococcus aureus growth and its enterotoxin production in milk, and vibrio parahaemolyticus growth in broth at various temperature patterns. the program, which was built with microsoft excel (visual basic application), is user-friendly; users can easily input ... | 2006 | 17228795 |
| vibrio alginolyticus infections in the netherlands after swimming in the north sea. | 2006 | 17213549 | |
| pore formation of thermostable direct hemolysin secreted from vibrio parahaemolyticus in lipid bilayers. | vibrio parahaemolyticus secretes thermostable direct hemolysin (tdh), a major virulence factor. earlier studies report that tdh is a pore-forming toxin. however, the characteristics of pores formed by tdh in the lipid bilayer, which is permeable to small ions, remain to be elucidated. ion channel-like activities were observed in lipid bilayers containing tdh. three types of conductance were identified. all the channels displayed relatively low ion selectivity, and similar ion permeability. the c ... | 2006 | 16940013 |
| response of vibrio parahaemolyticus to ethanol shock. | in this study, the growth and survival of vibrio parahaemolyticus in the presence of 0.0-8.0% ethanol was first examined. v. parahaemolyticus was then exposed to a sub-lethal dose of 5.0% ethanol for 30 and 60 min (ethanol shock). morphological changes and alterations in cell leakage, thermal tolerance at 47 degrees c, and susceptibility to 8% ethanol and low temperature (4 and -18 degrees c) of v. parahaemolyticus caused by ethanol shock were investigated. in addition, recoveries of the ethanol ... | 2006 | 16943038 |
| visible-light-induced bactericidal activity of a nitrogen-doped titanium photocatalyst against human pathogens. | the antibacterial activity of photocatalytic titanium dioxide (tio(2)) substrates is induced primarily by uv light irradiation. recently, nitrogen- and carbon-doped tio(2) substrates were shown to exhibit photocatalytic activities under visible-light illumination. their antibacterial activity, however, remains to be quantified. in this study, we demonstrated that nitrogen-doped tio(2) substrates have superior visible-light-induced bactericidal activity against escherichia coli compared to pure t ... | 2006 | 16957236 |
| quorum sensing-disrupting brominated furanones protect the gnotobiotic brine shrimp artemia franciscana from pathogenic vibrio harveyi, vibrio campbellii, and vibrio parahaemolyticus isolates. | autoinducer 2 (ai-2) quorum sensing was shown before to regulate the virulence of vibrio harveyi towards the brine shrimp artemia franciscana. in this study, several different pathogenic v. harveyi, vibrio campbellii, and vibrio parahaemolyticus isolates were shown to produce ai-2. furthermore, disruption of ai-2 quorum sensing by a natural and a synthetic brominated furanone protected gnotobiotic artemia from the pathogenic isolates in in vivo challenge tests. | 2006 | 16957276 |
| rapid estimation of microbial numbers in water using bulk fluorescence. | enumeration of microbial cells without culturing is an essential technique for microbial ecology and water quality evaluation. here we show that bulk fluorescence using the sybr gold dna stain can be used to rapidly quantify microbial cells per millilitre in fresh, marine and estuarine waters. the bulk fluorescence method is comparable to estimating cell concentrations in cultures using optical density; however, this enhanced method enables the user to estimate microbial numbers at lower concent ... | 2006 | 16958758 |
| development of monoclonal antibodies for simple identification of vibrio alginolyticus. | the present study was aimed to produce monoclonal antibodies (mabs) for simple and specific identification of vibrio alginolyticus infection in shrimp. | 2006 | 16965376 |
| response of spinach and komatsuna to biogas effluent made from source-separated kitchen garbage. | recycling of kitchen garbage is an urgent task for reducing public spending and environmental burdens by incineration and/or landfill. there is an interesting regional effort in ogawa, saitama prefecture, japan, in which source-separated kitchen garbage is anaerobically fermented with a biogas plant and the resultant effluent is used as a quick-release organic fertilizer by surrounding farmers. however, scientific assessments of fertilizer values and risks in the use of the effluent were lacking ... | 2006 | 16973635 |
| bacterial lateral flagella: an inducible flagella system. | flagella are complex surface organelles that allow bacteria to move towards favourable environments and that contribute to the virulence of pathogenic bacteria through adhesion and biofilm formation on host surfaces. there are a few bacteria that possess functional dual flagella systems, such as vibrio parahaemolyticus, some mesophilic aeromonas spp., rhodospirillum centenum and azospirillum brasilense. these bacteria are able to express both a constitutive polar flagellum required for swimming ... | 2006 | 16978346 |
| vp1686, a vibrio type iii secretion protein, induces toll-like receptor-independent apoptosis in macrophage through nf-kappab inhibition. | vibrio parahaemolyticus, causative agent of human gastrointestinal diseases, possesses several virulent machineries including thermostable direct hemolysin and type iii secretion systems (ttss1 and -2). in this report, we establish that ttss1-dependent secretion and translocation of a v. parahaemolyticus effector protein vp1686 into the cytosol induces dna fragmentation in macrophages. we performed yeast two-hybrid screening to identify the molecules involved in vp1686-mediated cell death pathwa ... | 2006 | 16984916 |
| two type iv pili of vibrio parahaemolyticus play different roles in biofilm formation. | vibrio parahaemolyticus rimd2210633 has two sets of type iv-a pilus genes. one set is similar to that found in other gram-negative bacteria, such as pseudomonas aeruginosa, vibrio cholerae (chitin-regulated pilus; chirp), and vibrio vulnificus. the other is homologous to the genes for the mannose-sensitive hemagglutinin (msha) pilus. in this study, we analyzed the effects of the deletions in the pilin genes for each type iv pilus (the chirp and the msha pilus) on biofilm formation. although the ... | 2006 | 17020553 |
| outbreak of gastroenteritis caused by the pandemic vibrio parahaemolyticus o3:k6 in mexico. | during 2003 and during late september of 2004, more than 1230 cases of gastroenteritis were reported in the south of sinaloa state, north-western mexico. all cases were attributed to the consumption of raw or undercooked shrimp collected at the huizache-caimanero lagunary system. vibrio parahaemolyticus was identified by standard biochemical methods, and many strains were positive for pcr amplifications of the tlh and tdh genes and negative for the trh gene. a representative strain belonged to t ... | 2006 | 17107421 |
| response of bdellovibrio and like organisms (balos) to the migration of naturally occurring bacteria to chemoattractants. | a dual culture-based and non-culture-based approach was applied to characterize predator bacterial groups in surface water samples collected from apalachicola bay, florida. chemotaxis drop assays were performed on concentrated samples in an effort to isolate predator bacteria by their chemotactic ability. yeast extract (ye) and casamino acids (ca) proved to be strong chemoattractants and resulted in three visibly distinct bands; however, dextrose, succinate, pyruvate, and concentrated cells of v ... | 2006 | 17115104 |
| involvement of the vibrio parahaemolyticus pvsc gene in export of the siderophore vibrioferrin. | the pvsc gene of unknown function has been found in the iron-regulated vibrioferrin biosynthesis operon of vibrio parahaemolyticus (tanabe, t. et al., j. bacteriol. 185: 6938-6949, 2003). the amino acid sequence deduced from the gene showed significant similarity to 12-transmembrane segment efflux pumps belonging to the major facilitator superfamily. a nonpolar deletion of pvsc in v. parahaemolyticus resulted in a reduced release of vibrioferrin into the medium. vibrioferrin release could be reg ... | 2006 | 17116982 |
| incidence of enteric bacterial pathogens in water found at the bottom of commercial freezers in calabar, southeastern nigeria. | bacteriological analysis of water that accumulates at the bottom of freezers in restaurants when the power was cut in calabar, southeastern nigeria, was carried out using standard procedures. mean heterotrophic bacterial counts and escherichia coli counts ranged from 3.1 +/- 0.02 to 7.1 +/- 0.30 x 10(4) cfu/ml and 0.2 +/- 0.10 to 0.6 +/- 0.50 x 10(4) cfu/ml, respectively, indicating heavy bacterial contamination whose source was mostly fecal. there was no significant difference (p > 0.05, 0.01) ... | 2006 | 17125005 |
| analysis of bacteria degradation products of methyl parathion by liquid chromatography/electrospray time-of-flight mass spectrometry and gas chromatography/mass spectrometry. | the biodegradation of the organophosphorus insecticide methyl parathion (mp) in aqueous environment by bacteria isolated from river sediment has been studied. two species of bacteria which show strong mp degradation ability are identified as shewanella and vibrio parahaemolyticus. the biodegradation of mp proceeded rapidly with the formation of a series of intermediate products, which were analyzed using a combination of gc/ms and hplc/esi-tofms techniques. the major products tentatively identif ... | 2006 | 17126847 |
| distribution of virulent and pandemic strains of vibrio parahaemolyticus in three molluscan shellfish species (meretrix meretrix, perna viridis, and anadara granosa) and their association with foodborne disease in southern thailand. | distribution of pandemic strains of vibrio parahaemolyticus in seafood, particularly in molluscan shellfish, and their serological and molecular relationships to clinical strains were examined from hat yai city in southern thailand. during 2000 to 2002, virulent strains (tdh+ or trh+) were isolated from 13 of 230 molluscan shellfish samples using alkaline peptone water enrichment followed by immunomagnetic separation. the isolates included 12 pandemic strains (tdh+, trh-, group-specific pcr posi ... | 2006 | 17133804 |
| occurrence and density of vibrio parahaemolyticus in live edible crustaceans from markets in china. | vibrio parahaemolyticus is a marine bacterium causing foodborne disease. occurrence of the bacterium was investigated in six species of edible crustaceans available from markets in mainland china. the bacterium was detected in 22 of 45 whole-body, shell, and feces samples, including mitten crabs, which are supposed to be produced in freshwater ponds. the mean densities ranged from 2.8 log cfu/g in mitten crabs (eriocheir sinensis) to 5.1 cfu/g in giant tiger prawns (penaeus monodon). in hemolymp ... | 2006 | 17133820 |
| evaluation of an alkaline phosphatase-labeled oligonucleotide probe for the detection and enumeration of the thermostable-related hemolysin (trh) gene of vibrio parahaemolyticus. | reliable methods are needed to detect total and pathogenic vibrio parahaemolyticus. one marker of v. parahaemolyticus virulence is the thermostable-related hemolysin. we developed an alkaline phosphatase-labeled dna probe method for the specific detection and enumeration of trh-positive v. parahaemolyticus by colony hybridization. the probe was tested against a panel of 200 bacterial strains and determined to be specific for trh-positive v. parahaemolyticus. additionally, the trh alkaline phosph ... | 2006 | 17133826 |
| influence of vibrio parahaemolyticus mtcc 451 on the levels of ascorbic acid and histamine in penaeus monodon (fabricius). | juvenile shrimps (penaeus monodon) weighing about 15-20 gms were procured from the grow-out ponds and reared under laboratory conditions. they were challenged with vibrio parahaemolyticus mtcc 451 and their ld50 value was evaluated. the ascorbic acid and the histamine activity of both the control and experimental groups were assessed. the results showed a marked decline in both the parameters at all intervals. this reveals the pathogenesis of the inoculated bacterial strain, which affects the de ... | 2006 | 16850878 |
| [molecular typing on vibrio parahaemolyticus isolates from hangzhou, china, during 2000-2002]. | to study the molecular epidemiology of vibrio parahaemolyticus isolates from clinical and environmental samples collected in hangzhou area during 2000 and 2002. | 2006 | 16875541 |
| sensitivities of foodborne pathogens to pressure changes. | eight foodborne pathogens were suspended in ultrahigh-temperature whole milk and treated at pressure levels of 0.1 to 690 mpa at 21.5 degrees c for 10 min. there was no clear trend in pressure resistance between gram-negative and gram-positive organisms. the order of the single strains tested, from most to least pressure sensitive, was vibrio parahaemolyticus < yersinia enterocolitica < listeria monocytogenes < salmonella enterica serovar typhimurium < s. enterica serovar enteritidis < escherich ... | 2006 | 16416910 |
| genomic comparisons among gamma-proteobacteria. | predicted highly expressed (phx) genes are compared for 16 gamma-proteobacteria and their similarities and differences are interpreted with respect to known or predicted physiological characteristics of the organisms. predicted highly expressed genes often reflect the organism's predominant lifestyle, habitat, nutrition sources and metabolic propensities. this technique allows to predict principal metabolic activities of the microorganisms operating in their natural habitats. among our findings ... | 2006 | 16423015 |
| analysis of the lateral flagellar gene system of aeromonas hydrophila ah-3. | mesophilic aeromonas strains express a polar flagellum in all culture conditions, and certain strains produce lateral flagella on semisolid media or on surfaces. although aeromonas lateral flagella have been described as a colonization factor, little is known about their organization and expression. here we characterized the complete lateral flagellar gene cluster of aeromonas hydrophila ah-3 containing 38 genes, 9 of which (lafa-u) have been reported previously. among the flgll and lafa structu ... | 2006 | 16428388 |
| identification of proteins secreted via vibrio parahaemolyticus type iii secretion system 1. | vibrio parahaemolyticus, a gram-negative marine bacterium, is an important pathogen causing food-borne gastroenteritis or septicemia. recent genome sequencing of the rimd2210633 strain (a kanagawa phenomenon-positive clinical isolate of serotype o3:k6) revealed that the strain has two sets of gene clusters that encode the type iii secretion system (ttss) apparatus. the first cluster, ttss1, is located on the large chromosome, and the second, ttss2, is on the small chromosome. previously, we repo ... | 2006 | 16428750 |
| differences in the api 20e biochemical patterns of clinical and environmental vibrio parahaemolyticus isolates. | genetic differences in clinical and environmental strains of vibrio parahaemolyticus have been widely used as criteria in identifying pathogenic isolates. however, few studies have been carried out to assess the differences in biochemical characteristics of v. parahaemolyticus isolates from human and environmental sources. we compared the biochemical profiles obtained by the characterization of v. parahaemolyticus isolates from human infections and the marine environment using the api 20e system ... | 2006 | 16436064 |
| salmonella spp., vibrio spp., clostridium perfringens, and plesiomonas shigelloides in marine and freshwater invertebrates from coastal california ecosystems. | the coastal ecosystems of california are highly utilized by humans and animals, but the ecology of fecal bacteria at the land-sea interface is not well understood. this study evaluated the distribution of potentially pathogenic bacteria in invertebrates from linked marine, estuarine, and freshwater ecosystems in central california. a variety of filter-feeding clams, mussels, worms, and crab tissues were selectively cultured for salmonella spp., campylobacter spp., escherichia coli-o157, clostrid ... | 2006 | 16897302 |
| vibrio parahaemolyticus infections associated with consumption of raw shellfish--three states, 2006. | during may 20-july 31, 2006, new york city, new york state, oregon, and washington health departments reported a total of 177 cases of vibrio parahaemolyticus infection, of which 122 have been associated with 17 clusters. a cluster has been defined as a group of two or more ill persons who were linked to the same shellfish source (e.g., shared a meal at the same restaurant or obtained shellfish from the same seafood market). certain clusters were associated with restaurants, certain clusters wit ... | 2006 | 16902397 |
| structure of the class iv adenylyl cyclase reveals a novel fold. | the crystal structure of the class iv adenylyl cyclase (ac) from yersinia pestis (yp) is reported at 1.9 a resolution. the class iv ac fold is distinct from the previously described folds for class ii and class iii acs. the dimeric ac-iv folds into an antiparallel eight-stranded barrel whose connectivity has been seen in only three previous structures: yeast rna triphosphatase and two proteins of unknown function from pyrococcus furiosus and vibrio parahaemolyticus. eight highly conserved ionic ... | 2006 | 16905149 |
| identification of vibrio harveyi using pcr amplification of the toxr gene. | the aim of this study was to develop an effective method for the identification of vibrio harveyi based on using the toxr gene as a taxonomic marker. | 2006 | 16910927 |
| [isolation of thermostable direct hemolysin producing vibrio parahaemolyticus from food using screening by pcr in food-borne outbreaks]. | the producibility of thermostable direct hemolysin (tdh) is the most important pathogenic factor in vibrio parahaemolyticus. tdh (+) v. parahaemolyticus is usually isolated from patients having v. parahaemolyticus food-borne disease. tdh (+) v. parahaemolyticus is, however, very difficult to isolate from food and environmental samples. in the 5 years from 2000 to 2004 in tokyo, v. parahaemolyticus was isolated from food samples related to 67 of 227 v parahaemolyticus food-borne outbreaks. in the ... | 2006 | 16922481 |
| detection of pandemic vibrio parahaemolyticus o3:k6 serovar in gulf of mexico water and shellfish using real-time pcr with taqman fluorescent probes. | we describe a real-time multiplexed pcr method using taqman probes for the detection of total and pandemic vibrio parahaemolyticus o3:k6 serovar in oysters and gulf of mexico water (gulf water). the specificity of these primers and probes was tested for amplification of a 450 bp thermolabile hemolysin (tlh) and a 369 bp orf8 amplicon representing all v. parahaemolyticus and post-1996 clinical isolates of pandemic serovar o3:k6, respectively. the sensitivity of detection was 10 pg purified dna or ... | 2006 | 16923074 |
| bactericidal effects of wine on vibrio parahaemolyticus in oysters. | the bactericidal effects of wines on vibrio parahaemolyticus in oysters were studied to evaluate potential inactivation of v. parahaemolyticus in contaminated oysters by wine consumption. shucked whole oyster and oyster meat homogenate were inoculated with v. parahaemolyticus and mixed with red or white wine. survivals of v. parahaemolyticus in inoculated oysters were determined at 7 and 25 degrees c. populations of v. parahaemolyticus in inoculated whole oysters (5.52 log most probable number [ ... | 2006 | 16924905 |
| effects of electrolyzed oxidizing water treatment on reducing vibrio parahaemolyticus and vibrio vulnificus in raw oysters. | contamination of vibrio parahaemolyticus and vibrio vulnificus in oysters is a food safety concern. this study investigated effects of electrolyzed oxidizing (eo) water treatment on reducing v. parahaemolyticus and v. vulnificus in laboratory-contaminated oysters. eo water exhibited strong antibacterial activity against v. parahaemolyticus and v. vulnificus in pure cultures. populations of v. parahaemolyticus (8.74 x 10(7) cfu/ml) and v. vulnificus (8.69 x 10(7) cfu/ml) decreased quickly in eo w ... | 2006 | 16924906 |
| [study on the antibacterial mechanisms of copper-bearing montmorillonite]. | vibrio parahaemolyticus atcc 27519 was chosen as indicator of aquacultural pathogenic bacteria to determine the antibacterial activity and mechanism of copper-bearing montmorillonite (cu-mmt) in vitro. the results indicated that montmorillonite (mmt) had no antibacterial activity. the minimum inhibitory concentration (mic) and bactericidal concentration (mbc) of cu-mmt on vibrio parahaemolyticus were 75 and 300 mg/l, respectively. the activities of aspartate aminotransferase (ast), alanine amino ... | 2006 | 16933615 |
| antibacterial activity of plant extracts from azuki beans (vigna angularis) in vitro. | this study was undertaken to examine the antimicrobial property of azuki beans (vigna angularis). the water extracts of green, black and red colored azuki beans showed antibacterial effects against staphylococcus aureus, aeromonas hydrophila and vibrio parahaemolyticus. in contrast, the extract of white azuki beans showed no inhibition towards any of the microorganisms examined. the extracts of colored azuki beans contained larger amounts of polyphenols including proanthocyanidins than the extra ... | 2006 | 16444673 |
| a reinvestigation of the secondary structure of functionally active vsglt, the vibrio sodium/galactose cotransporter. | the bacterial na(+)/galactose cotransporter vsglt of vibrio parahaemolyticus is a member of the sodium:solute symporter family (sss). previous studies using electron microscopy have shown that vsglt is a monomeric protein. computational and experimental topological analyses have consistently indicated that this protein possesses 14 transmembrane alpha-helices. our previous study using attenuated total reflectance fourier transform infrared spectroscopy (atr-ftir) to quantitate secondary structur ... | 2006 | 16445289 |
| antiviral activities of purified compounds from youngia japonica (l.) dc (asteraceae, compositae). | the ethanol extract of a biannual medicinal herb, youngia japonica (commonly known as oriental hawk's beard) was reported previously to have potent antiviral activity against respiratory syncytial virus (rsv) cultured in hep-2 cells. three anti-microbial agents, namely 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, and luteolin-7-o-glucoside were subsequently purified and chemically characterized from the ethanol extract of youngia japonica. the two dicaffeoylquinic acids exhibited promin ... | 2006 | 16469463 |
| occurrence of foodborne pathogenic bacteria in retail prepackaged portions of marine fish in spain. | to survey the presence of indigenous and nonindigenous foodborne bacterial pathogens in displayed prepacked portions of fresh marine fish. | 2006 | 16478492 |
| application of real-time pcr for quantitative detection of vibrio parahaemolyticus from seafood in eastern china. | vibrio parahaemolyticus is recognized as a leading human food-borne pathogen. a taqman pcr assay based on the gyrase b gene (gyrb) sequence of v. parahaemolyticus was developed for quantitative detection of v. parahaemolyticus in seafood. the study involving 27 v. parahaemolyticus and 10 strains of other species indicated that the real-time pcr test was highly specific. the sensitivity of the assay was approximately a single cfu per pcr in pure culture and six to eight cfu per pcr in spiked raw ... | 2006 | 16487299 |
| variation in the 16s-23s rrna intergenic spacer regions in vibrio parahaemolyticus strains are due to indels nearby their trnaglu. | vibrio parahaemolyticus contains 11 rrna operons each including one of six 16s-23s rrna gene intergenic spacer classes differing in size and nucleotide sequence. some of the spacer classes may differ between isolates. we observed that the differences in the spacers between isolates are generally in two spacer classes present in single copies in the genome, one class containing trna(ala) and trna(glu) and the other trna(glu) exclusively. moreover, these differences are due to indels located nearb ... | 2006 | 16487317 |
| immunostimulation of white shrimp (litopenaeus vannamei) following dietary administration of ergosan. | ergosan an algal product containing 1% alginic acid, developed for use in aquaculture and reported to have immunomodulatory activity, was administered orally to intermoult adult white shrimp (litopenaeus vannamei) for 15 days. examination of haemolymph proteins using sds-page did not reveal any obvious differences between control and ergosan treated shrimp. similarly, total haemocyte counts were found to be roughly equivalent for both the control and experimental samples. however, differential a ... | 2006 | 16490202 |
| [indicative vibrio parahaemolyticus strains for the detection and primary indentification of parahemolytic phages]. | 2006 | 16512482 | |
| cyclo(phe-pro) modulates the expression of ompu in vibrio spp. | vibrio vulnificus was found to produce a chemical that induced the expression of vibrio fischeri lux genes. electron spray ionization-mass spectrometry and 1h nuclear magnetic resonance analyses indicated that the compound was cyclo(l-phe-l-pro) (cfp). the compound was produced at a maximal level when cell cultures reached the onset of stationary phase. sodium dodecyl sulfate-polyacrylamide gel analysis of the total proteins of v. vulnificus indicated that expression of ompu was enhanced by exog ... | 2006 | 16513751 |
| detection of vibrio parahaemolyticus in shellfish by use of multiplexed real-time pcr with taqman fluorescent probes. | we developed a multiplexed real-time pcr assay using four sets of gene-specific oligonucleotide primers and four taqman probes labeled with four different fluorophores in a single reaction for detection of total and pathogenic vibrio parahaemolyticus, including the pandemic o3:k6 serotype in oysters. v. parahaemolyticus has been associated with outbreaks of food-borne gastroenteritis caused by the consumption of raw or undercooked seafood and therefore is a concern to the seafood industry and co ... | 2006 | 16517652 |
| antimicrobial effect of spices and herbs on vibrio parahaemolyticus. | the antimicrobial effects of spices and herbs from 18 plant species were examined on a foodborne pathogen, vibrio parahaemolyticus, with the use of combinations of temperatures and nutrient levels. basil, clove, garlic, horseradish, marjoram, oregano, rosemary, and thyme exhibited antibacterial activities at incubation of 30 degrees c, while with the exception of horseradish, the same spices and additional 7 species exhibited the activities at 5 degrees c. the lowest mic (minimum inhibitory conc ... | 2006 | 16797760 |
| new cluster of plasmid-located class 1 integrons in vibrio cholerae o1 and a dfra15 cassette-containing integron in vibrio parahaemolyticus isolated in angola. | the resistance profile and its correlation with mobile genetic elements were investigated in 11 vibrio cholerae o1 and 2 vibrio parahaemolyticus clinical isolates, as well as in 1 v. cholerae o1 and 1 v. cholerae non-o1 environmental isolate, isolated between 1991 and 1996 in different provinces of angola. all clinical isolates of v. cholerae o1 were resistant to ampicillin, chloramphenicol, trimethoprim, sulfamethoxazole, and tetracycline. they also contained a large conjugative plasmid (p3iang ... | 2006 | 16801431 |
| antibacterial activity of citrus fruit juices against vibrio species. | lemon, lime and sudachi juices were tested for antibacterial activity against seven strains of vibrio species. all juices were effective in inhibiting the growth of the vibrio strains. citric acid, the major organic acid in these juices, was found to be responsible for inhibiting the growth of vibrio parahaemolyticus. sauce prepared from sudachi juice showed a strong bactericidal activity against vibrio parahaemolyticus, whereas the sauce adjusted to higher ph values had no bacterial activity. d ... | 2006 | 16802698 |
| genetic richness of vibriophages isolated in a coastal environment. | the purpose of this study was to characterize vibrio parahaemolyticus viruses (vpvs) isolated from different environments within and adjacent to the strait of georgia, and to examine the relative influences of distance and environment on host-range and genetic richness. nearly all seawater enrichment cultures (29/31) generated isolates, implying that vpvs were widespread in the virioplankton, yet at low abundances (< 1 l(-1)). viruses were not detected in sediments (n = 99). fourteen of the 16 v ... | 2006 | 16817925 |
| detection of free-living and plankton-bound vibrios in coastal waters of the adriatic sea (italy) and study of their pathogenicity-associated properties. | culturable vibrios were isolated from water and plankton fractions collected during an 18-month sampling study performed along the north-central coast of the adriatic sea (italy). unculturable vibrio vulnificus and v. parahaemolyticus were detected in plankton fractions by polymerase chain reaction amplification of dna sequences for cytotoxin-haemolysin and thermolabile haemolysin respectively. the presence of v. parahaemolyticus, v. vulnificus and v. cholerae virulence genes and the expression ... | 2006 | 16817938 |
| soft-agar-coated filter method for early detection of viable and thermostable direct hemolysin (tdh)- or tdh-related hemolysin-producing vibrio parahaemolyticus in seafood. | a novel method for detecting viable and thermostable direct hemolysin (tdh)-producing or tdh-related hemolysin (trh)-producing vibrio parahaemolyticus in seafood was developed. the method involved (i) enrichment culture, selective for viable, motile cells penetrating a soft-agar-coated filter paper, and (ii) a multiplex pcr assay targeting both the tdh gene (tdh) and trh gene (trh) following dnase pretreatment on the test culture to eradicate any incidental dnas that might have been released fro ... | 2006 | 16820446 |
| inhibition of vibrio parahaemolyticus by a bacteriocin-like inhibitory substance (blis) produced by vibrio mediterranei 1. | the aim of this research was to identify and partially purify new bacteriocin-like substances from strains of halophilic 'non-cholera' vibrios isolated from food sources. | 2006 | 16834611 |
| pyrolysis mass spectrometry for distinguishing potential hoax materials from bioterror agents. | pyrolysis mass spectrometry (pyms) was investigated as a rapid tool to distinguish potential bioterror hoax materials from samples containing pathogenic bacteria. a pyrolysis time-of-flight (tof) mass spectrometer equipped with an alternative ionization technique, metastable atom bombardment (mab), was used to produce sample spectra. these spectra were analyzed by principal component and discriminant analysis for pattern recognition. materials investigated were two strains of vibrio parahaemolyt ... | 2006 | 16841357 |
| vibrio parahaemolyticus, vibrio vulnificus and microorganisms of fecal origin in mussels (mytilus galloprovincialis) sold in the puglia region (italy). | mytilus galloprovincialis is one of the most commonly consumed of all bivalve molluscs. the consumption of raw bivalve molluscs has caused outbreaks of food poisoning due to vibrio parahaemolyticus and vibrio vulnificus. this paper reports the results of a survey on the presence of v. parahaemolyticus, v. vulnificus fecal coliform bacteria, escherichia coli and salmonella spp. in 600 m. galloprovincialis samples collected from retail outlets in the puglia region. v. parahaemolyticus and v. vulni ... | 2006 | 16226820 |
| white shrimp (litopenaeus vannamei) recombinant lysozyme has antibacterial activity against gram negative bacteria: vibrio alginolyticus, vibrio parahemolyticus and vibrio cholerae. | c-type lysozyme has been described as an antibacterial component of the shrimp innate defence system. we determined quantitatively the antibacterial activity of white shrimp (litopenaeus vannamei) recombinant lysozyme against three gram negative bacteria: vibrio alginolyticus, vibrio parahemolyticus and vibrio cholerae, using a turbidimetric assay with live bacteria and differential bacterial viable count after interaction with the protein. in conclusion, the antibacterial activity of recombinan ... | 2006 | 16098764 |
| isolation of vibrio parahaemolyticus and vibrio vulnificus from wild aquatic birds in japan. | vibrio parahaemolyticus and vibrio vulnificus were isolated from faecal samples of wild aquatic birds in winter. although v. parahaemolyticus and v. vulnificus were present in low numbers in seawater in the area where the faecal samples of the birds were collected, the pathogens were isolated from the faeces of the birds. this study demonstrates that wild aquatic birds are a vehicle for v. parahaemolyticus and v. vulnificus to survive in winter. | 2006 | 16371182 |
| cloning, functional expression and primary characterization of vibrio parahaemolyticus k+/h+ antiporter genes in escherichia coli. | the regulation of internal na(+) and k(+) concentrations is important for bacterial cells, which, in the absence of na(+) extrusion systems, cannot grow in the presence of high external na(+). likewise, bacteria require k(+) uptake systems when the external k(+) concentration becomes too low to support growth. at present, we have little knowledge of k(+) toxicity and bacterial outward-directed k(+) transport systems. we report here that high external concentrations of k(+) at alkaline ph are tox ... | 2006 | 16390457 |
| isolation and molecular characterization of toxigenic vibrio parahaemolyticus from the kii channel, japan. | studies were conducted on the ecology of potentially pathogenic vibrio parahaemolyticus in three coastal areas of kii channel, tokushima, japan. seawater and seaweed samples were collected seasonally between june 2003 and may 2004. total and toxigenic strains of v. parahaemolyticus were isolated using most probable number culture and colony blot hybridization. toxigenic strains were serotyped and further characterized by random amplified polymorphic dna (rapd) and ribotyping. six thousand strain ... | 2006 | 16338587 |
| sanitation of seawater effluent from seaweed processing plants using a photo-catalytic tio2 oxidation. | a fine porcelain open-cell photo-catalytic filter with titanium dioxide (tio(2)) was evaluated for sterilization and sanitation of bio-polluted industrial water. in simulated seawater industrial effluent samples, the populations of escherichia coli and vibrio parahaemolyticus quickly decreased and reached non-detectable level within 10min. in seawater effluents from a seaweed processing plant, the bacterial populations in two samples quickly decreased by more than 10(3). in another two samples t ... | 2006 | 16325650 |
| vibrio parahaemolyticus in shellfish and clinical samples during two large epidemics of diarrhoea in southern chile. | large epidemics of diarrhoea associated with seafood consumption and vibrio parahaemolyticus occurred during the austral summers of 2004 and 2005 in the environs of puerto montt, chile (41 degrees 29's 72 degrees 24'w). there are no reports of v. parahaemolyticus infections before 2004 in this region, their absence being explained by the low ocean temperatures which seldom reach 16 degrees c. we analysed v. parahaemolyticus obtained from shellfish and clinical samples during epidemics. isolates ... | 2006 | 16584479 |
| occurrence of vibrio parahaemolyticus, v. cholerae, and v. vulnificus in norwegian blue mussels (mytilus edulis). | vibrio parahaemolyticus, v. cholerae, and v. vulnificus were isolated from 10.3%, 1.0%, and 0.1% of 885 blue mussel samples, respectively. four of the samples contained trh(+) v. parahaemolyticus, while no tdh-positive isolates were detected. the v. cholerae isolates were non-o:1/non-o:139 serotypes and were ctxa negative. | 2006 | 16598019 |
| biochemical fingerprinting of vibrio parahaemolyticus by the pheneplate system: comparison between pandemic and non-pandemic serotypes. | during recent years a pandemic clone of vibrio parahaemolyticus has emerged. isolates of this clone are distributed among several serotypes, but are genotypically related. in the present study, a phenotyping method (biochemical fingerprinting) was used to characterize pandemic and non-pandemic isolates belonging to v. parahaemolyticus. it was found that the pandemic isolates showed a high level of phenotypic homogeneity and a majority of the pandemic isolates belonged to the same biochemical phe ... | 2006 | 16650334 |
| four genomic islands that mark post-1995 pandemic vibrio parahaemolyticus isolates. | vibrio parahaemolyticus is an aquatic, halophilic, gram-negative bacterium, first discovered in 1950 in japan during a food-poisoning outbreak. infections resulting from consumption of v. parahaemolyticus have increased globally in the last 10 years leading to the bacterium's classification as a newly emerging pathogen. in 1996 the first appearance of a pandemic v. parahaemolyticus clone occurred, a new o3:k6 serotype strain that has now been identified worldwide as a major cause of seafood-born ... | 2006 | 16672049 |
| development and evaluation of a rapid, simple, and sensitive immunochromatographic assay to detect thermostable direct hemolysin produced by vibrio parahaemolyticus in enrichment cultures of stool specimens. | thermostable direct hemolysin (tdh) is considered to be a major virulence factor in vibrio parahaemolyticus, and most cases of v. parahaemolyticus diarrhea in humans are caused by tdh gene-positive strains. in the present study, we developed an immunochromatographic assay to detect tdh (tdh-ica) and evaluated the utility of tdh-ica for the diagnosis of v. parahaemolyticus diarrhea. tdh-ica allowed the detection of 0.2 ng/ml of tdh within 10 min. fecal homogenates were spiked with various numbers ... | 2006 | 16672412 |