Publications

serological survey for selected diseases in the endangered san joaquin kit fox (vulpes macrotis mutica).	blood from endangered san joaquin kit foxes (vulpes macrotis mutica) inhabiting the elk hills naval petroleum reserve, kern county, and the elkhorn plain, san luis obispo county, california, was collected in 1981, 1982 and 1984 and sera were tested for antibodies against 10 selected pathogens. proportions of kit fox sera containing antibodies against pathogens were: canine parvovirus, 100% in 1981-1982 and 67% in 1984; infectious canine hepatitis virus, 6% in 1981-1982 and 21% in 1984; canine di ...	1988	2836636
genetic analysis of the iron uptake region of the vibrio anguillarum plasmid pjm1: molecular cloning of genetic determinants encoding a novel trans activator of siderophore biosynthesis.	clones carrying the iron uptake region of the vibrio anguillarum plasmid pjm1 were subjected to insertion mutagenesis, using transposon tn3::hoho1 which carries a promoterless lacz gene and can thus generate lacz transcriptional fusions if inserted downstream from an indigenous promoter. four classes of insertion mutants were obtained based on the level of expression of components of the iron uptake system, and six genetic units were defined according to the phenotype of the mutants. five of the ...	1988	2832388
genetic analysis of the iron uptake region of the vibrio anguillarum plasmid pjm1: molecular cloning of genetic determinants encoding a novel trans activator of siderophore biosynthesis.	clones carrying the iron uptake region of the vibrio anguillarum plasmid pjm1 were subjected to insertion mutagenesis, using transposon tn3::hoho1 which carries a promoterless lacz gene and can thus generate lacz transcriptional fusions if inserted downstream from an indigenous promoter. four classes of insertion mutants were obtained based on the level of expression of components of the iron uptake system, and six genetic units were defined according to the phenotype of the mutants. five of the ...	1988	2832388
epigenetic control and reintegration of extrachromosomal proviral dna in hl60 cells chronically infected with human t cell leukaemia virus type 1.	extrachromosomal human t cell leukaemia virus type 1 (htlv-i) proviruses are persistently maintained in htlv-i-infected human promyelocytic leukaemia (hl60) cells even 24 months after viral infection. by successive recloning of these htlv-i-infected clones, and by southern blot analysis of their htlv-i proviruses, we concluded the following. the copy number of extrachromosomal proviruses fluctuated, and this fluctuation was probably dependent on the epigenetic conditions in the host cell, hl60. ...	1988	2832528
viruses in sewage waters during and after a poliomyelitis outbreak and subsequent nationwide oral poliovirus vaccination campaign in finland.	during an outbreak of paralytic poliomyelitis in finland in 1984 and 1985 the widespread circulation of the causative wild-type serotype 3 poliovirus in the population was documented by demonstrating the virus in sewage water specimens in 13 different locations in the greater helsinki district and in 13 other cities or towns all over the country. after the nationwide campaign with oral poliovirus vaccine in 1985, poliovirus serotypes 2 and 3 were readily isolated from sewage waters for up to 2 m ...	1988	2833160
herpes simplex virus infection of the hand. a profile of 79 cases.	seventy-nine cases of herpes simplex (hsv) infection of the hand were documented by viral culture over a 40-month period. a marked bimodal age distribution was found with 32 cases occurring in adults aged 21 to 30 years and 16 occurring in children less than 10 years old. the infection most commonly occurred on the fingers (69 percent) and thumb (21 percent). all 20 herpes simplex type 2 (hsv-2) isolates came from patients over 20 years old. all of the 13 specimens that were typed after isolatio ...	1988	2827469
steroid regulation of somatostatin mrna in the rat hypothalamus.	the participation of gonadal steroid hormones in the regulation of the expression of the somatostatin gene in the hypothalamus and cerebral cortex was studied by using a quantitative densitometric hybridization assay which allows the direct measurement of specific somatostatin mrna levels. the levels of somatostatin mrna in hypothalamus were found to be significantly decreased following gonadectomy in both male and female rats (67% in males and 75% in females). moreover, with in situ hybridizati ...	1988	2897361
analysis of esmolol in human blood by high-performance liquid chromatography and its application to pharmacokinetic studies.		1988	2897375
regional levels of neurotransmitter markers in the pigeon telencephalon: a comparison with possibly homologous areas of the rat telencephalon.	the levels of cholinergic, gamma-aminobutyric acidergic (gabaergic), and excitatory amino acid neurotransmitter markers have been measured in 18 regions of the pigeon telencephalon as well as in supposedly homologous areas of the rat telencephalon. among the basal telencephalic areas, some similar patterns of regional distribution were observed, with the noticeable exception of the ratio of levels of cholinergic markers between the striatum and globus pallidus, which was much larger in the rat t ...	1988	2897425
the high molecular weight receptor to transforming growth factor-beta contains glycosaminoglycan chains.	proteoglycans are constituents of the cell surface that may play important roles in the regulation of cell behavior. here we report that the 250-kda receptor subunit that binds the multifunctional protein, transforming growth factor-beta 1 (tgf-beta 1), contains chains of heparan sulfate and chondroitin sulfate and thus is a proteoglycan. digestion of tgf-beta 1-receptor complexes with glycosaminoglycan (gag)-specific degradative enzymes yield core proteins of 115-140 kda. cell monolayers that h ...	1988	2897367
inhibitory effects of atropine and adrenergic antagonists on the changes in autonomic receptors and cyclic nucleotides of rat parotid and submandibular glands caused by sympathetic nerve stimulation.	the changes in cyclic amp (camp) concentration and density of beta-adrenoceptors caused by electrical stimulation of the sympathetic innervation to parotid and submandibular glands of rat did not occur when the alpha- and beta-adrenergic antagonists, phentolamine, and propranolol were administered 20 min prior to initiation of stimulation. they also did not occur when phentolamine, the beta-adrenergic antagonist, was administered alone prior to nerve stimulation, indicating that beta-adrenocepto ...	1988	2895130
[double control of pili formation in yersinia pestis].		1988	2907926
ca2+-dependent and ca2+-independent glutamate release, energy status and cytosolic free ca2+ concentration in isolated nerve terminals following metabolic inhibition: possible relevance to hypoglycaemia and anoxia.	hypoglycaemia and anoxia both cause massive release of glutamate from the brain in vivo, and the nature of this release was investigated using guinea-pig cerebral-cortical synaptosomes and iodoacetate and rotenone to simulate the energetic consequences of these conditions. glutamate release (by continuous fluorimetry), cytoplasmic free ca2+ (by fura-2), membrane potentials, atp, adp and creatine phosphate were determined in parallel, following the addition of iodoacetate or rotenone, alone or in ...	1988	2904664
[effects of prazosin, sgb-1534, dobutamine and isoproterenol on congestive heart failure in dogs].	effects of prazosin, dobutamine, isoproterenol and sgb-1534, a new alpha-blockade, on congestive heart failure (chf) in dogs were investigated. the model was made by protease injection into the left ventricular free wall, saline loading, and dextran and methoxamine infusions. by this maneuver, left atrial pressure (lap), systemic vascular resistance (svr) and left ventricular end-diastolic pressure (lvedp) were markedly increased, aortic blood flow (aobf) was decreased, and systemic blood pressu ...	1988	2906315
[ridge augmentation with controlled porous structure hydroxyapatite (aps-7) granule in tooth extraction cavity].		1988	2855431
inhibition of porphyrin biosynthesis by exogenous 5-aminolevulinic acid in an aerobic photosynthetic bacterium, erythrobacter sp. och 114.	exogenously administered 5-aminolevulinic acid (ala) inhibited the formation of bacteriochlorophyll a (bchl a) in a dose-dependent manner in the aerobic photosynthetic bacterium, erythrobacter sp. strain och 114, under dark growth conditions. the ala concentration required for half-inhibition after 24-h growth was estimated to be about 3.0 mm. porphyrin and bchl precursors were not found in either the cells or the growth medium. the same inhibition was also observed with cytochrome c formation. ...	1988	2847654
dual control of the intracellular ph in aortic smooth muscle cells by a camp-sensitive hco3-/cl- antiporter and a protein kinase c-sensitive na+/h+ antiporter.	two mechanisms are involved in the regulation of the intracellular ph (phi) of aortic smooth muscle cells: the na+/h+ antiporter and a na+-independent hco3-/cl- antiporter. the na+/h+ antiporter acts as a cell alkalinizing mechanism. it is activated by vasopressin and by phorbol esters when cells are incubated in the presence of bicarbonate but is not affected in the absence of bicarbonate. the hco3-/cl- antiporter acts as a cell acidifying mechanism. agents such as forskolin, 8-br-camp, and iso ...	1988	2848021
characterization of receptors for platelet-activating factor on platelets, polymorphonuclear leukocytes and macrophages.	1. we have compared the potency of the putative platelet-activating factor (paf) receptor antagonists (web 2086, l-652,731 and bn 52021) against paf-induced aggregation of rabbit and guinea-pig platelets, aggregation of rabbit polymorphonuclear leukocytes (pmnls) and prostacyclin generation by guinea-pig resident peritoneal macrophages. 2. on rabbit washed platelets and pmnls web 2086, l-652,731 and bn 52021 each antagonized competitively paf-induced aggregation. the rank order of potency was we ...	1988	2850058
[changes in the cyclic nucleotide system of t-lymphocytes in experimental tuberculosis in animals].		1988	2851783
sex as a risk factor for htlv-i spread among intravenous drug abusers.		1988	2895250
[effect of bilirubin and serum albumin on the na,k-atpase activity in the synaptosomal membrane].	the absorption spectrum of visible light, characteristic of the free bilirubin being in the aqueous medium, with a single maximum at 440 nm and with the shoulder in the region of 410-420 nm is transformed into the spectrum with two maxima in the region of 460 and 500 nm, respectively, when the pigment is bound in vitro by the synaptosomal membrane. there are two types of sites for bilirubin binding in the membrane particles, differing in the values of constants of association (ka = 0.6 . 10(5) a ...	1988	2847380
activities of amikacin, roxithromycin, and azithromycin alone or in combination with tumor necrosis factor against mycobacterium avium complex.	the effect of amikacin and two new macrolides (roxithromycin and azithromycin) used either alone or in combination with recombinant tumor necrosis factor (tnf) to inhibit or kill mycobacterium avium complex in human macrophages was examined in vitro. macrophage monolayers infected with m. avium complex (strain 101, serotype 1) were treated with antibiotics or tnf by using three different protocols: (i) antibiotics or tnf was added to the monolayers immediately after infection and washed out afte ...	1988	2847644
activities of amikacin, roxithromycin, and azithromycin alone or in combination with tumor necrosis factor against mycobacterium avium complex.	the effect of amikacin and two new macrolides (roxithromycin and azithromycin) used either alone or in combination with recombinant tumor necrosis factor (tnf) to inhibit or kill mycobacterium avium complex in human macrophages was examined in vitro. macrophage monolayers infected with m. avium complex (strain 101, serotype 1) were treated with antibiotics or tnf by using three different protocols: (i) antibiotics or tnf was added to the monolayers immediately after infection and washed out afte ...	1988	2847644
activation of calpain i and calpain ii: a comparative study using terbium as a fluorescent probe for calcium-binding sites.	the present study demonstrates the activation of calpain i and calpain ii by micromolar levels of terbium and has utilized the enhancement in the fluorescence of protein-bound terbium to study and compare the calcium binding sites of the two enzymes. calpain i and calpain ii were isolated from bovine erythrocytes and brain, respectively. while the rates of activation of calpain i by terbium and calcium are comparable, the rate of activation of calpain ii was much greater in the presence of terbi ...	1988	2847653
human placental endonuclease cleaves holliday junctions.	a partially purified endonuclease from human placenta cleaves cruciform structures. the placental enzyme is active both on extruded cruciform structures from negatively supercoiled covalently closed circular plasmid dna and on synthetic x-junctions formed by reannealing short oligonucleotides. plasmids containing natural or cloned palindromes such as pbr322 and phd101-3 were used as substrates. the synthetic x-junction tetramer dna formed by reannealing short oligonucleotides, was converted into ...	1988	2847712
characterization of vaccinia virus dna topoisomerase i expressed in escherichia coli.	the putative structural gene encoding the vaccinia virus type i dna topoisomerase (ec 5.99.1.2) was expressed in escherichia coli under the control of a bacteriophage t7 promoter. provision of t7 rna polymerase resulted in the accumulation to high level of a mr = 33,000 type i topoisomerase with the properties of the vaccinia enzyme. a simple purification scheme yielded approximately 8 mg of recombinant vaccinia topoisomerase from 400 ml of bacteria. dna unwinding by the enzyme was stimulated by ...	1988	2846543
effects of gossypol on the activity of kidney (na+ + k+)-atpase and the functions of erythrocyte membrane.	gossypol extracted from cottonseed oil, reputed to exert contraceptive action in males, may induce a side effect of hypokalemia. the cellular and molecular mechanisms by which gossypol produces hypokalemia are not quite understood. we have examined the inhibitory effect of gossypol on the activity of (na+ + k+)-atpase purified from the outer medulla of rabbit kidney, the half maximal inhibitory concentration (ic50) is 6.5 micron. the kinetic studies using this enzyme preparation show that gossyp ...	1988	2836126
efficacy of the neuropeptide org.2766 in the prevention and treatment of cisplatin-induced neurotoxicity in rats.	in rats chronic systemic treatment with cisplatin results in a sensory neuropathy as evidenced by a reduction in the sensory conduction velocity in the sciatic nerve. concomitant administration of the neurotrophic acth4-9 analog, org.2766, prevents the occurrence of the neuropathy. in addition, treatment with org.2766 stops further deterioration and improves recovery of an already established cisplatin-induced neuropathy. furthermore, concomitant administration of org.2766 during a first cisplat ...	1988	2838292
efficacy of a combination thromboxane receptor antagonist and lipoxygenase inhibitor in traumatic shock.	the effects of a thromboxane receptor antagonist having lipoxygenase inhibitory activity, l-655,240 (3-[1-(4-chlorobenzyl)-5-fluoro-3-methyl-indol-2-yl]2,2-dimethylpropa noic acid) (1 mg/kg per h) were studied in a standardized model of traumatic shock. pentobarbital (35 mg/kg) anesthetized rats subjected to noble-collip drum trauma were characterized by a 82 +/- 12 min survival time, a 20-fold increase in plasma cathepsin d activity, and a 6-fold increase in plasma myocardial depressant factor ...	1988	2845544
characterization of vaccinia virus dna topoisomerase i expressed in escherichia coli.	the putative structural gene encoding the vaccinia virus type i dna topoisomerase (ec 5.99.1.2) was expressed in escherichia coli under the control of a bacteriophage t7 promoter. provision of t7 rna polymerase resulted in the accumulation to high level of a mr = 33,000 type i topoisomerase with the properties of the vaccinia enzyme. a simple purification scheme yielded approximately 8 mg of recombinant vaccinia topoisomerase from 400 ml of bacteria. dna unwinding by the enzyme was stimulated by ...	1988	2846543
efficacy of the neuropeptide org.2766 in the prevention and treatment of cisplatin-induced neurotoxicity in rats.	in rats chronic systemic treatment with cisplatin results in a sensory neuropathy as evidenced by a reduction in the sensory conduction velocity in the sciatic nerve. concomitant administration of the neurotrophic acth4-9 analog, org.2766, prevents the occurrence of the neuropathy. in addition, treatment with org.2766 stops further deterioration and improves recovery of an already established cisplatin-induced neuropathy. furthermore, concomitant administration of org.2766 during a first cisplat ...	1988	2838292
preparation of heteroenzyme conjugates: trypsin-chymotrypsin and trypsin-alkaline phosphatase.	hybrid enzymes which have two different enzyme activities linked together covalently may be useful reagents for various applications, such as the determination of complex biological structures. the present paper describes the preparation and purification of two such enzyme-enzyme conjugates, namely, trypsin-chymotrypsin and trypsin-alkaline phosphatase. whereas the former has been prepared by using the well-known bifunctional reagent glutaraldehyde, the latter exploited the schiff base formation ...	1988	2840096
efficacy of a combination thromboxane receptor antagonist and lipoxygenase inhibitor in traumatic shock.	the effects of a thromboxane receptor antagonist having lipoxygenase inhibitory activity, l-655,240 (3-[1-(4-chlorobenzyl)-5-fluoro-3-methyl-indol-2-yl]2,2-dimethylpropa noic acid) (1 mg/kg per h) were studied in a standardized model of traumatic shock. pentobarbital (35 mg/kg) anesthetized rats subjected to noble-collip drum trauma were characterized by a 82 +/- 12 min survival time, a 20-fold increase in plasma cathepsin d activity, and a 6-fold increase in plasma myocardial depressant factor ...	1988	2845544
cell-specific activity of the human immunodeficiency virus enhancer repeat in vitro.	the binding of nuclear proteins and the functional activity of the hiv-ltr enhancer repeats in different cell lines (jurkat, cem, h9, u937, raji, b cells, t47d, hela, 293, and hepg2 cells) was investigated in vitro. five distinct complexes formed with the enhancer repeat have been identified by an electrophoretic mobility shift assay. the distribution of these complexes varied qualitatively and quantitatively between nuclear proteins from different sources. in the extracts tested, transcription ...	1988	2848558
patch-clamp recording of amino acid-activated responses in "organotypic" slice cultures.	patch-clamp recording techniques were used to study the properties of amino acid-activated channels in cultured "organotypic" slices from rat cerebellum and hippocampus. hippocampal pyramidal cells responded to the three main glutamatergic agonists, n-methyl-d-aspartate (n-me-d-asp), quisqualate, and kainate, whereas purkinje cells responded only to quisqualate and kainate. analysis of single-channel events recorded in outside-out patches from hippocampal neurons showed large conductance events ...	1988	2834737
use of progress curves to estimate the co-substrate-to-substrate flow ratio of a symport mechanism. application to the isoleucine-na+ symport of mouse ascites-tumour cells and to the lactose-proton symport.	the model envisages two components in the process, whereby ht equivalents of co-substrate and st equivalents of substrate accumulate in the cellular compartment in time t. the first is the flow through the symport, n equivalents of co-substrate entering or leaving with each substrate equivalent. the second is the basal flow of co-substrate outside the symport. in certain specific circumstances n can be derived by plotting ht/t against st/t. the principal requirement is that, whereas the ratio of ...	1988	2839154
virus-pesticide interactions with murine cellular immunity after sublethal exposure to dieldrin and aminocarb.	interaction of two potential immunosuppressive factors, sublethal pesticide exposure and viral inhibition of lymphocyte mitogenesis, was examined in mixed lymphocyte reaction (mlr). inbred (c57bl/6 x a/j)f mice, semisusceptible to mouse hepatitis virus 3 (mhv3) infection were exposed to selected pesticides and subsequently infected with the mhv3 virus. the mortality of animals was examined as a function of pesticide exposure. two pesticides were selected for further studies: the organochlorine p ...	1988	3418741
effects of age on the motor unit. a study on single motor units in the rat.		1988	3364890
[an immunoenzyme monoclonal test system for detecting the causative agents of plague].		1988	2462124
[a dry culture medium for determining the cellular composition of strains of the plague agent based on its requirements for calcium ions].		1988	2463423
molecular evolution of the human and simian immunodeficiency viruses.	molecular evolution and phylogeny of different human immunodeficiency virus type 1 (hiv1) strains, of a type 2 (hiv2) strain, and of two simian immunodeficiency viruses (sivagm and sivmac) have been studied by comparing the nucleotide sequences of the two regions of their pol genes which encode the reverse transcriptase (rt) and endonuclease/integrase (en). the analyses show that the different hiv 1s form one cluster (hiv1 group) and that the sivs and hiv2 form another (hiv2 group). when the ent ...	1988	2464734
[development of an immunoenzyme method for determining yersinia pestis 43000 antigen].		1988	2457734
the effect of unilateral naris occlusion on cell dynamics in the developing rat olfactory epithelium.	the effect of unilateral naris occlusion on the cellular dynamics of developing olfactory epithelium was investigated in postnatal rats. nares of rat pups, at 1, 5, and 10 d postnatally, were cauterized; after a 30 d survival period, the olfactory mucosa was examined histologically, and quantitative estimates were made of total number of receptor neurons (together with basal cells), supporting cells, and epithelial thickness. in each group, there were significant differences between occluded and ...	1988	2459323
[quantitative determination of specific plague fluorescent immunoglobulin activity using pulse flow cytofluorometry].		1988	2466163
heterogeneity in chromosome damage and repair rates after bleomycin in ataxia telangiectasia cells.	cells derived from patients with ataxia telangiectasia (at) are known to be exceptionally sensitive to ionizing radiation and chemotherapeutic agents such as bleomycin (blm), neocarzinostatin, and etoposide. this increased sensitivity is manifested by high chromosome aberration frequencies after treatment. in order to probe the underlying basis for this phenomenon, the technique of premature chromosome condensation was used to determine whether the increased chromosome damage observed after bleo ...	1988	2446745
the kinetics of human granulopoiesis following treatment with granulocyte colony-stimulating factor in vivo.	cell proliferation in the bone marrow and blood of two patients with metastatic breast cancer who were treated with granulocyte colony-stimulating factor was studied by using [3h]thymidine labeling and autoradiography. additionally, the fate of neutrophils labeled with 99mtc-hexamethylpropyleneamineoxime was observed following granulocyte colony-stimulating factor infusion. proliferation increased in all stages of granulopoiesis, but a significant amount of the increased production stemmed from ...	1989	2480603
[control of anticoagulant therapy by using soluble thromboplastin].	the characteristics of soluble thromboplastin, a new coagulant agent, were under study. this drug is a high-molecular lipoprotein complex of human cadaver brain, purified by gel chromatography. the new preparation has been standardized and compared to thromboplastin preparations manufactured in this country and abroad. soluble thromboplastin has been found fairly sensitive to reductions of the prothrombin factors levels, it may be used for monitoring the efficacy of indirect anticoagulant therap ...	1989	2481064
[isolation of monoclonal antibodies for the identification of strains of the causative agent of plague that do not contain a capsular antigen].	in most cases the immunological identification of y. pestis strains is based on the use of capsular antigen as an immunological marker. however, there are y. pestis strains without capsular antigen. for the immunological identification of such strains, homogeneous antigen with a molecular weight of 43 kd has been isolated and monoclonal antibodies to it have been obtained. the enzyme-linked immunosorbent assay, carried out with the use of these monoclonal antibodies and intended for the detectio ...	1989	2511708
direct activation of human polymorphonuclear leukocytes by pseudomonas aeruginosa.	brief exposure of polymorphonuclear leukocytes (pmns) to unopsonized pseudomonas aeruginosa resulted in significant enhancement of pmn adherence and 3h-deoxyglucose uptake, two important indicators of pmn activation. dose-response and time-course experiments show that maximal stimulation of pmns occurred at a pmn: bacteria ratio of 10:1 and exposure time of 10 min. the pmn-stimulatory capacity of p. aeruginosa was completely or partially abolished by treatment with formalin, heat, ultrasonicatio ...	1989	2515158
[evaluation of 2 methods for studying slime production by coagulase-negative staphylococcus strains].	the surface hydrophobicity and the slime production of 46 coagulase negative staphylococci isolated from the urine, blood and skin were evaluated. the hydrophobicity values were very heterogeneous and did not correlate with the species, origin or slime production. slime production was evaluated by the classical tube method and by a micromethod with spectrophotometric readings. there was a high correlation between both methods, but the results were more reproducible and quantifiable with the latt ...	1989	2490721
lack of effect by prostaglandin f2 alpha and verapamil on calcium uptake by isolated corpora lutea from pseudopregnant rats.		1989	2492809
endothelial cells from human umbilical vein secrete functional transcobalamin ii.	transcobalamin ii (tcii) is a cobalamin (cbl) binding protein in the plasma that mediates the cellular uptake of cbl. although the synthesis of tcii by a variety of cultured mammalian cells and by some isolated perfused organs has been reported, no single tissue has been identified as the source of tcii in vivo. in this study, we demonstrate that cultured human umbilical vein endothelial cells secrete a protein that binds cn[57co]cbl, elutes from a sephacryl s-200 column in the same position as ...	1989	2493194
experimental transmission of crimean-congo hemorrhagic fever virus by hyalomma truncatum koch.	larval hyalomma truncatum ticks were infected with crimean-congo hemorrhagic fever (cchf) virus by allowing them to engorge on viremic newborn mice. the overall tick infection rate was 4.4% (24/542). virus was detected in specimens for greater than or equal to 160 days postinfection. transstadial transmission to the adult tick stage was observed and horizontal transmission to a mammalian host was demonstrated. horizontal transmission of cchf virus to uninfected adult ticks occurred while feeding ...	1989	2493203
monitoring of intestinal transplant rejection.	our results show that maltase, sucrase, and lactase activity are present at a normal level in nonrejecting small bowel transplants after an initial postoperative decline. this confirms that the disaccharide absorbing capacity of these grafts is intact. in allogeneic bowel, however, the levels of maltase and sucrase decline as histologic rejection occurs. these results suggest that serial maltase, sucrase, and possibly lactase levels in allogeneic intestinal transplants may serve as a useful adju ...	1989	2493205
in vitro susceptibility of feline herpesvirus-1 to vidarabine, idoxuridine, trifluridine, acyclovir, or bromovinyldeoxyuridine.	in vitro activities of 9-[( 2-hydroxyethoxy] methyl) guanine (acyclovir), (e)-5-(2-bromovinyl)-2'deoxyuridine, 9-beta-d-arabinofuranosyladenine (vidarabine), 5-iodo-2'-deoxyuridine (idoxuridine), and 5-trifluoromethyl-2'-deoxyuridine (trifluridine) were studied against 6 strains of feline herpesvirus-1. a significant difference was not detected among viral strains in their susceptibility to these compounds (p = 0.442). the relative potency of these compounds was trifluridine much greater than id ...	1989	2493210
the gene coding for the 190,000-dalton iron-regulated protein of yersinia species is present only in the highly pathogenic strains.	a genomic library containing dna fragments of 0.5 to 2 kilobase pairs in length from yersinia enterocolitica serovar o:8 was constructed in a bacteriophage lambda gt11 expression vector. mouse antibodies specific for the iron-regulated high-molecular-weight proteins (hmwps) were used to screen the library. two positive clones of 1 and 0.5 kilobase pairs, designated a13 and d7, respectively, were detected and isolated. they coded for beta-galactosidase fusion proteins of 151,000 and 138,000 dalto ...	1989	2466794
[the agar precipitation reaction as a method of selecting fraction-free strains of yersinia pestis].		1989	2468040
bile acid profile in a dog with cholangiocarcinoma.		1989	2536512
[a method of fixation and disinfection of microbial cells on magnetic sorbents].	the 96% ethanol has been found fairly effective for the fixation and disinfection of yersinia pestis, vibrio cholerae, pseudomonas mallei, and pseudomonas pseudomallei after their contact with magnetic polyacrylamide sorbents containing specific immunoglobulins for ligands. such step is recommended for the quantitative immunofluorescence test employed for rapid analysis, diagnosis, and identification of the above microorganisms.	1989	2474712
[methodology of determining yersinia pestis antigens using dot-immunologic analysis].	the technique of dot-immunoassay on nitrocellulose filters is described for the detection of the vital infection (vi) agents. the results are read visually (qualitatively) and instrumentally (quantitatively). the method is highly sensitive (3.2 +/- 0.8 ng/ml of fraction i) and specific, cost-effective, simple, and available for laboratories engaged in vi diagnosis.	1989	2474713
mechanisms of inhibitory noradrenergic transmission in the rabbit facial vein.	in isolated buccal segment of the rabbit facial vein, electrical responses produced by perivascular nerve stimulation and exogenously applied noradrenaline (na) were recorded from the smooth muscle cells using microelectrode. perivascular nerve stimulation hyperpolarized the smooth muscle cell membrane. the hyperpolarization was converted to depolarization after application of the beta-adrenoceptor antagonist, propranolol, and the depolarization was blocked by alpha 2-adrenoceptor antagonists, y ...	1989	2538798
selective radioiodination of the apical and luminal cell surfaces: in vitro and in situ experiments on vascular endothelial cells with iodogen-coated sephadex.	a gentle and nonexpensive agent for selective radioiodination of the cell surface proteins was obtained by plating aliquots of iodogen on dried sephadex beads 50-60 microns in diameter. iodogen-coated sephadex inherits iodogen properties: it is stable and virtually insoluble in water, allowing rapid iodination of the cell surface proteins in the solid phase with 125i-. iodination is terminated by simply removing the beads. the agent was tested on bovine aortic endothelial cells in culture and on ...	1989	2476041
[isolation of high-affinity antibodies from hyperimmune serum].	a group of high-affinity antibodies has been isolated from horse hyperimmune antiplague serum with the use of antigenic immunosorbent. high-affinity antibodies have proved to be homogeneous, which is indicated by the rectilinear character of the graph of the inverse values of the free antigen concentration.	1989	2476900
molecular analysis of lcrgvh, the v antigen operon of yersinia pestis.	the lcrgvh operon of plasmid pcd1 in yersinia pestis kim encodes the virulence-associated v antigen, the regulatory protein lcrh, and lcrg, a protein of undefined function. in this study we sequenced lcrgvh and analyzed it for transcription initiation sites. there were three open reading frames within the sequence, 288, 981, and 507 bases in length, which could encode proteins with molecular weights and isoelectric points corresponding to those of lcrg, lcrv (v antigen), and lcrh, respectively. ...	1989	2477361
differential immunohistochemical localization of cytokeratins and collagen types i and iii in experimentally-induced cirrhosis.	liver cirrhosis was induced in male wistar rats by subcutaneous injection (1 ml of 30 g/l) of an aqueous solution of thioacetamide. using the indirect immunoperoxidase technique, high molecular weight keratins were localized in bile ducts and ductules. low molecular weight cytokeratins were present in regenerating hepatocytes in active cirrhosis; bile ducts were unstained. these results suggest that cytokeratin staining may be useful in distinguishing bile duct epithelium and hepatocytes in hepa ...	1989	2478687
[immunoelectrophoretic analysis of the antigenic composition of yersinia].	the antigenic composition of 24. y. pseudotuberculosis newly isolated and reference strains, 7 y. enterocolitica strains, as well as y. pestis vaccine strain ev, has been studied by the method of immunoelectrophoresis in agar. the antigenic composition of these bacteria has been found to be complicated and to comprise not less than 8-11 antigens, and among them nonspecific protein antigens common for enterobacteria, the common generic antigen, the antigen common with y. pestis, as well as o-anti ...	1989	2480038
[natural infection by yersinia pestis, in fleas from plague foci in the northeast of brazil].	three different containment transport processes of fleas were evaluated as an approach to the bacteriologic isolation of yersinia pestis. the three methods employed were: live fleas in glass tubes containing pieces of wrapped filter paper; dead fleas in saline solution; and macerated fleas in cary-blair culture medium. the two latter methods were almost equal and superior to the first method. a total of 29512 flea pools, from plaque foci in northeast brazil collected during 1966 to 1982 were eva ...	1989	2487774
alexandre yersin: pursuer of plague.		1989	2494200
chloride and osmotic water permeabilities of isolated rabbit renal papillary surface epithelium.	the papillary surface epithelium (pse) covers the mammalian renal papilla. it has been proposed that water or solute fluxes across the pse might result in changes in urine or medullary interstitial fluid composition. to study osmotic water and chloride permeabilities, the pse was dissected from underlying medullary tissue and mounted in a small ussing chamber. osmotic water permeability was low (14.2 +/- 3.0 microns/s) and was unaffected by 100 nm vasopressin added to the basolateral surface. in ...	1989	2504054
nuclear membrane lipid peroxidation products bind to nuclear macromolecules.	ascorbate-fe2+-driven lipid peroxidation processes in isolated rat liver nuclei give rise to products that bind to dna and total nuclear proteins. this has been demonstrated by integrating [3h]arachidonic acid into the nuclear membranes. lipid peroxidation was estimated from the formation of 2-thiobarbituric acid chromophore, and from the relative distribution of 3h-peroxidation products between the lipidic fraction and the nonlipidic fraction of the nuclear suspensions during incubation. the am ...	1989	2493218
detection of neisseria meningitidis and yersinia pestis with a novel silicon-based sensor.	a light-addressable potentiometric (silicon) sensor was used in an immunofiltration procedure for the detection of pathogenic bacteria. yersinia pestis was detected by filtering the cells onto nitrocellulose membranes and then filtering anti-y. pestis mouse monoclonal antibody and anti-mouse immunoglobulin g-horseradish peroxidase conjugate. for neisseria meningitidis detection, mouse monoclonal antibody to the major outer membrane protein of this bacterium was coupled directly to horseradish pe ...	1989	2504767
differential effects of the destruction of leydig cells by administration of ethane dimethane sulphonate to postnatal rats.	ethane dimethane sulphonate (eds) is a cytotoxic drug that selectively destroys leydig cells in adult testes. this study has examined the effect of a single injection of eds on the leydig cell populations present in the testes of rats aged 5, 10, or 20 days. microscopic examination of the tissue demonstrated that the fetal leydig cell population was destroyed at all ages, but that subsequent development of the adult population of leydig cells was not affected. whilst the destruction of the fetal ...	1989	2546615
[studies of the antigenic structure of trichophyton equinum].	in the studies on antigenic structure of t. equinum double diffusion precipitation test in agar gel and immunoelectrophoresis were used. while preparing precipitation sera in rabbits it was manifested that the best results are achieved by immunization with live antigen. in the group of metabolic antigens and water extracts of the strains of t. equinum which were tested, 10-11 serologically+ active fractions were found whereas in protein component only l1 fraction. in comparative studies based on ...	1989	2518619
the effect of cyclosporin a in murine visceral leishmaniasis.	leishmania donovani is an obligate intracellular protozoan parasite of macrophages whose control is effected by cellular mechanism. in order to determine the role of t-helper cells and their soluble mediators in the control of the infection, the effects of cyclosporin a (csa) were studied in strains of mice (c57l/j and c57bl/6j) known to be resistant and susceptible. mice treated with csa (200 mg/kg) exhibited as early as day 15 a significant increase in the level of their infection. the number ...	1989	2525802
regulation of hepatic expression of igf i and fetal igf binding protein mrna in streptozotocin-diabetic rats.	hepatic mrna levels of insulin-like growth factor i (igf i) and of the fetal, nonglycosylated 32 kda igf-binding protein (bp) were analysed in diabetic, diabetic insulin- and igf i-treated rats as well as in age-matched, healthy control animals. igf imrna levels are reduced in diabetic rats and increased by insulin treatment. in contrast, the infusion of igf i does not significantly upregulate igf i mrna levels. fetal igf bp mrna expression is very low in healthy control animals, but high levels ...	1989	2546824
does dietary fibre stimulate intestinal epithelial cell proliferation in germ free rats?	the aim of the present experiment was to investigate the role of hind gut fermentation in the proliferative response of the intestinal epithelium to dietary fibre. we have previously shown that refeeding starved rats with an elemental diet supplemented with fermentable dietary fibre (but not inert bulk) is capable of stimulating intestinal epithelial cell proliferation throughout the gastrointestinal tract. three groups of 10 germ free (gf) rats and three groups of 10 conventional (cv) rats, wer ...	1989	2546871
a yersinia pestis-specific dna fragment encodes temperature-dependent coagulase and fibrinolysin-associated phenotypes.	the effect of temperature on coagulase and fibrinolysin expression (pla) by yersinia pestis has been implicated in the transmission of plague by fleas. in an attempt to improve our understanding of this process, we have cloned, sequenced and characterized the gene encoding the pla phenotypes in y. pestis, and examined its temperature-dependent regulation. the coding region for this gene overlaps a 900bp y. pestis-specific dna fragment that we have previously shown to be capable of detecting plag ...	1989	2526282
the role of thromboxane a2 in experimental and human models of chronic glomerular damage.		1989	2526467
characterization of three functional sites in alpha beta 1 dr of drw13. all three sites are potentially involved in major histocompatibility complex-peptide interaction.	an hla-dr product encoded by the hla-drw13/dw19 haplotype has been identified as the hla class ii molecule involved in antigen presentation to several influenza-specific helper t cell clones. three different functional sites were identified on this molecule by comparing the structure of hla-dr products of known sequences and their ability to efficiently present foreign antigen to the t cell clones. these functional sites were mapped on the recently proposed three-dimensional structure of hla cla ...	1989	2529124
long-term analysis of data on the isolation of yersinia pestis cultures from rodents during epizootological examinations of natural foci in muyun-kum and eastern kizil-kum.	results are presented of a long-term examination and evaluation of data on the microbiological procedures of isolating yersinia pestis cultures from wild mammals and their association animal plague pathogenesis as suggested by investigations in the muyun-kum autonomous plague focus and the eastern kizil-kum mesofocus. 1772 yersinia pestits cultures were isolated largely from rhombomys opimus as the principle host over 23 years. the authors determined the frequency of pathological alterations in ...	1989	2533934
[assessment of diagnostic prospects for a method of immunoenzyme analysis during mass testing of feces of patients for the purpose of detecting rotavirus antigens].		1989	2555970
effect of glucocorticoids and limiting nursing on the carbohydrate digestive capacity and growth rate of piglets.	the influence of glucocorticoid administration and limited nursing on piglet carbohydrase enzyme development and subsequent growth was examined in three experiments using 371 piglets. treatments in the first two experiments were formed by the factorial arrangement of hydrocortisone (-hyd or +hyd) and limited nursing (-ln or +ln) imposed form d 14 to weaning (d 28). hydrocortisone was replaced by adrenocorticotropic hormone (acth) in the third experiment. growth rates were severely depressed by h ...	1989	2556355
[acute maternal anterior poliomyelitis in a non-endemic zone].	the authors report the case of a 26 year old woman with acute anterior poliomyelitis contracted during the vaccination of her baby. despite having been herself vaccinated in infancy she was not protected against the poliovirus. the clinical interest of this uncommon case is a severe paralytic state with definitive paraplegia. the authors suggest serologic testing of patients born before 1967 especially if they are at risk of encountering the virus.	1989	2561040
outer membrane peptides of yersinia pestis mediating siderophore-independent assimilation of iron.	it is established that wild-type cells of yersinia pestis absorb exogenous hemin or congo red and thus grow as pigmented colonies at 26 degrees c on media containing these chromatophores (pgm+). pgm+ isolated are known to possess a siderophore-independent mechanism of iron-transport (required for growth in iron-deficient medium) which is absent in avirulent pgm- mutants. production of the bacteriocin pesticin and linked invasins (pst+) is an additional defined virulence factor of yersiniae; muta ...	1989	2535180
effect of endogenous opioid peptides on trh release from rat stomach in vitro.	the effect of endogenous opioid peptides (beta-endorphin, leucine-enkephalin, dynorphin 1-13 on the release of thyrotropin releasing hormone (trh) from the rat stomach in vitro was studied. the rat stomach was incubated in the medium 199 with 1.0 mg/ml of bacitracin (ph 7.4) (medium) and the amount of trh released into the medium was measured by radioimmunoassay. the release of trh from the rat stomach was inhibited significantly in a dose-related manner with the addition of endogenous opioid pe ...	1989	2565806
start and end of the effects of terfenadine and astemizole on histamine-induced wheals in human skin.	a study was made of effects of two antihistamines, terfenadine (60 mg twice daily) and astemizole (10 mg once daily) on wheals induced by histamine dihydrochloride (10 mg/ml) in the prick test on the upper back of 15 healthy students. the suppressive effects of terfenadine on the histamine wheal appeared earlier (2 h), and disappeared earlier (within 1 day) than those of astemizole (3 days and 28 days, respectively). no difference between the maximal effects of the two drugs was seen.	1989	2566243
the cd2 antigen associates with the t-cell antigen receptor cd3 antigen complex on the surface of human t lymphocytes.	t lymphocytes can be activated by various stimuli directed either against the t-cell antigen receptor-cd3 antigen complex (ti-cd3) or the cd2 molecule; see ref. 1 for a review. activation signals generated by antigen binding to the antigen-specific alpha/beta heterodimer (ti) are thought to be transduced via the invariant cd3 gamma, epsilon and delta chains, and the associated zeta and eta subunits. the physiological role of the interaction of cd2 with its homologous cell-surface associated liga ...	1989	2567497
cloning and structural characterization of the genes coding for adenosylcobalamin-dependent methylmalonyl-coa mutase from propionibacterium shermanii.	the structural genes coding for both subunits of adenosylcobalamin-dependent methylmalonyl-coa mutase from the gram-positive bacterium propionibacterium shermanii have been cloned, with the use of synthetic oligonucleotides as primary hybridization probes. the genes are closely linked and are transcribed in the same direction. nucleotide sequence analysis of 4.5 kb of dna encompassing both genes allowed us to infer the complete amino acid sequence of the two subunits: the beta-subunit is the pro ...	1989	2569861
gut perforation after infection with herpes simplex virus and epstein-barr virus.		1989	2570271
correlation between selective inhibition of the cyclic nucleotide phosphodiesterases and the contractile activity in human pregnant myometrium near term.	the present study was carried out to determine the ability of various pharmacological agents to selectively inhibit each cytosolic form of phosphodiesterase isolated from the longitudinal layer of human myometria near term. among the drugs tested, zaprinast specifically inhibits the first form of pde which hydrolyses both substrates (camp and cgmp) and is stimulated by the ca2+-calmodulin complex. a second form of pde specific for camp hydrolysis and ca2+-calmodulin insensitive is only present d ...	1989	2535936
vigabatrin therapy in patient with succinic semialdehyde dehydrogenase deficiency.		1989	2572834
modulation of transmitter and hormone release by multiple neuronal opioid receptors.		1989	2573137
adeno-associated virus rep protein synthesis during productive infection.	adeno-associated virus (aav) rep proteins mediate viral dna replication and can regulate expression from aav genes. we studied the kinetics of synthesis of the four rep proteins, rep78, rep68, rep52, and rep40, during infection of human 293 or kb cells with aav and helper adenovirus by in vivo labeling with [35s]methionine, immunoprecipitation, and immunoblotting analyses. rep78 and rep52 were readily detected concomitantly with detection of viral monomer duplex dna replicating about 10 to 12 h ...	1989	2536109
structure-activity relationships as a response to the pharmacological differences in beta-receptor ligands.	with a few notable exceptions, beta-receptor ligands (agonists and antagonists) belong to the aryl- or heteroaryl-ethanolamine series and to the aryl- or heteroaryl-oxypropanolamine series. structure-activity relationships for beta-adrenergic agonists show that a secondary amine in the phenylethanolamine side chain ending is essential for receptor stimulation. the 3,4-dihydroxyphenyl groups may be replaced by "phenol equivalents" (-ch2oh, -nhconh2, -choh, -nhso2ch3). in contrast, substitution at ...	1989	2573372
barium- and calcium-permeable channels open at negative membrane potentials in rat ventricular myocytes.	ca2+- and ba2+-permeable channel activity from adult rat ventricular myocytes, spontaneously appeared in the three single-channel recording configurations: cell-attached, and excised inside-out or outside-out membrane patches. single-channel activity was recorded at steady-state applied membrane potentials including the entire range of physiologic values, and displayed no "rundown" in excised patches. this activity occurred in irregular bursts separated by quiescent periods of 5 to 20 min in cel ...	1989	2478710
characterization of rat cerebral cortical beta adrenoceptor subtypes using (-)-[125i]-iodocyanopindolol.	(-)-[125i]-iodocyanopindolol [-)icyp), used to characterize beta adrenoceptors on membrane preparations from rat cerebral cortex, was shown to have affinity for both beta adrenoceptors and serotonin receptors. therefore, 10 microm serotonin was added to the assays to prevent (-)icyp binding to serotonin receptors. under these conditions, (-)icyp binding to the cortical membrane preparation was reversible and saturable, and the association reaction was very slow. the dissociation reaction was als ...	1989	2577008
[possible control of capsule formation and intracellular synthesis of envelope antigen by each different plasmid].	variants which lacked capsular envelopes on their cell surface were isolated from the culture of a highly virulent yreka strain of yersinia pestis grown in the presence of acridine orange, ethidium bromide or sodium dodecyl sulfate at incompletely growth-inhibitory concentrations. the variant could be divided into two types on the basis of the presence and the absence of intracellular envelope antigen. both types of the variants lacked the 13 megadaltone (md) plasmid. thus, it may well be said t ...	1989	2504836
[iron assimilation by yersinia pestis on iron-deficient media].	the growth of plague bacteria may be limited by the level of iron concentration in the nutrient medium. the virulent strains of the plague microbe possess the more pronounced mechanism of iron assimilation as compared to the vaccine strain. the iron ions are extracted by the virulent and vaccine strains only under the cell surface contact with the iron-saturated transferrin. the iron-sorbing function is peculiar to the plague microbe cell walls which is pronounced more strongly in the virulent s ...	1989	2524638
site specificity of the chorionic gonadotropin n-linked oligosaccharides in signal transduction.	the role of the human chorionic gonadotropin (hcg) n-linked oligosaccharides in receptor binding and signal transduction was analyzed using site-directed mutagenesis and transfection studies. hcg derivatives with alterations at individual glycosylation sites were expressed in chinese hamster ovary cells. receptor binding studies showed that absence of any or all of the hcg n-linked oligosaccharides had only a minor effect on the receptor affinity of the derivatives. similarly, absence of the n-l ...	1989	2536708
immunogenicity of a yeast-derived hepatitis b vaccine in hemodialysis patients.	in a multicenter study of hemodialysis patients in spain, the immunogenicity of a yeast-derived recombinant deoxyribonucleic acid hepatitis b vaccine was evaluated. two different vaccination schedules were examined: zero, one, two, six months and zero, one, two, 12 months. two different dose levels (20 micrograms and 40 micrograms) were also compared. no serious adverse effects were reported by any of the vaccinees; the most frequently reported reaction was soreness at the injection site. this s ...	1989	2528295