Publications

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type 2 iodothyronine deiodinase in skeletal muscle: effects of hypothyroidism and fasting.the iodothyronine deiodinases d1, d2, and d3 enable tissue-specific adaptation of thyroid hormone levels in response to various conditions, such as hypothyroidism or fasting. the possible expression of d2 mrna in skeletal muscle is intriguing because this enzyme could play a role in systemic as well as local t3 production.200919293265
bone mineral density, growth, and thyroid function in long-term survivors of pediatric hodgkin's lymphoma treated with chemotherapy only.the aim of this study was to investigate the long-term side effects of treatment for childhood hodgkin's lymphoma with chemotherapy only on growth, bone mineral density (bmd), body composition, and thyroid function.200919293271
bladder tumour staging: comparison of diffusion- and t2-weighted mr imaging.the aim of this work was to evaluate the clinical feasibility of diffusion-weighted (dw) mri in detection and staging of urinary bladder tumour and to compare dw mri with the t(2)-weighted technique. one hundred and six patients with bladder tumour were prospectively included in our study. all patients were evaluated with mr imaging. we started with axial t(2)-weighted high resolution mr of the urinary bladder, then dw mri. two radiologists independently interpreted the mr images, and discrepanc ...200919247665
bacterial populations in complementary foods and drinking-water in households with children aged 10-15 months in zanzibar, tanzania.bacteria were quantified in samples of drinking-water and in two porridges prepared for infant-feeding [fortified instant soy-rice porridge (srp) and cooked porridge (lishe bora, lb)] in 54 households. bacterial numbers were measured again after the porridges had been held at room temperature for four hours (t4). findings were benchmarked against bacterial numbers in traditional complementary foods sampled from 120 households. total bacteria, coliform, and enterobacteriaceae counts were enumerat ...200919248647
the evolution of management for inverted papilloma: an analysis of 200 cases.to assess the evolution of management within one institution with the largest case series and longest clinical follow-up of ip to date in the literature and to compare this management with what has been recently presented in publication.200919248937
dna delivery in vitro via surface release from multilayer assemblies with poly(glycoamidoamine)s.localized controlled release of nucleic acid therapeutics could be an effective way to reduce the extracellular barriers associated with systemic delivery. herein, we have used the layer-by-layer film deposition approach to construct ultrathin multilayer assemblies for in vitro controlled release of plasmid dna (pdna). layer-by-layer assemblies containing alternate layers of cationic poly(l-tartaramidopentaethylenetetramine) (t4), and anionic pdna were fabricated. the film thickness and the abso ...200919249723
radioactive iodine for hyperthyroidism in children and adolescents: referral rate and response to treatment.radioactive iodine ((131)i) therapy is increasingly viewed as a safe and effective treatment for paediatric and adolescent hyperthyroidism. our objective was to estimate treatment response and its predictors and describe current referral practices for (131)i therapy.200919250263
fermentative production of thymidine by a metabolically engineered escherichia coli strain.thymidine is an important precursor in the production of various antiviral drugs, including azidothymidine for the treatment of aids. since thymidine-containing nucleotides are synthesized only by the de novo pathway during dna synthesis, it is not easy to produce a large amount of thymidine biologically. in order to develop a host strain to produce thymidine, thymidine phosphorylase, thymidine kinase, and uridine phosphorylase genes were deleted from an escherichia coli bl21 strain to develop b ...200919251902
the effect of hormone replacement treatment on thrombin-activatable fibrinolysis inhibitor activity levels in patients with hashimoto thyroiditis.hypothyroid patients have increased risk of cardiovascular diseases, and several mechanisms have been considered responsible in these patients. although, a few studies demonstrated fibrinolytic system changes in hypothyroid patients, there is no study demonstrating tafi activity in hypothyroid hashimoto's thyroiditis patients. the aim of this study was to evaluate tafi activity status and the effect of l thyroxin hormone replacement treatment on fibrinolytic system in this patient group.200919252348
neoadjuvant chemotherapy and surgery for esophageal adenocarcinoma: prognostic value of circumferential resection margin and stratification of n1 category.in the uk, it is standard practice to treat esophageal adenocarcinoma with neoadjuvant chemotherapy (no radiation) and surgery. we examined the prognostic value of the status of the circumferential resection margin (crm) and stratification of the n1 category into 1-4 nodes or > or = 5 nodes.200919252949
rapid metabolic and newborn screening of thyroxine (t4) from dried blood spots by ms/ms.thyroxin (t(4)) plays an important role in the regulation of the rate of metabolism of proteins, fats, and carbohydrates. congenital hypothyroidism, an inherited deficiency of thyroid hormones, is screened in newborns using a variety of methods that include total t(4) or thyroid stimulating hormone (tsh). some laboratories measure either total t(4) or tsh as a means of detecting infants with hypothyroidism with a subset that analyzes both total t(4) and tsh as a primary screen or a combination o ...200919254705
development and evaluation of a structural model for sf1b helicase dda.helicases are proteins that unwind double-stranded nucleic acids. dda helicase from bacteriophage t4 has served as an excellent model for understanding the molecular mechanism of this class of enzymes. study of the structure of dda may reveal why some helicases translocate in a 5' to 3' direction on dna, while others translocate in a 3' to 5' direction. attaining a structure of dda has proven difficult because the protein fails to readily form crystals and is too large for current nmr technologi ...200919256528
thyreotropic effect of human chorionic gonadotropin (hcg) in mares at estrus.the beta-subunits of thyroid stimulating hormone (tsh) and human chorionic gonadotropin (hcg) share a high homology, just like the ectodomains of their receptors, do. as a consequence, hcg was shown to exert a thyrotropic action in humans and hamsters. this study aimed to investigate whether hcg, used to induce ovulation, displays a thyrotropic effect in the equine species too. forty mares at estrus were divided in two groups; 20 were intravenously treated with sterile saline solution (nacl 0.9% ...200919235540
effect of a bis-thiazolium compound on the biosynthesis of plasmodium falciparum phospholipids.in the eukaryotic cell, phospholipids can be biosynthesized by two pathways, one from choline and the other one from ethanolamine. the functional effectiveness of each pathway depends on the type of the cell. thiazolium designed-drugs have shown, under in vivo conditions, antiplasmodial and antimalarial activities with inhibition of the phospholipids biosynthesis. this study aimed to discover the pathways involved in the biosynthesis of phospholipids in plasmodium and deduce the biochemical step ...200919235542
is adjuvant chemotherapy beneficial to high risk stage ii colon cancer? analysis in a single institute.colorectal carcinoma is the most common malignancy of the gastrointestinal tract. it remains controversial for adjuvant chemotherapy in patients with stage ii colon cancer. this study was designed to identify the risk factors of tumor recurrence in stage ii colon cancer. furthermore, the benefit of adjuvant chemotherapy for high-risk stage ii colorectal cancer will be investigated.200919238405
isolation and characterization of bacteriophages for avian pathogenic e. coli strains.to isolate and characterize bacteriophages, and to evaluate its lytic performance against avian pathogenic escherichia coli (apec) strains with high patterns of antibiotic resistance, in order to select phages for a therapeutic product to treat colibacillosis in chickens.200919239552
evaluation of acceptability and use of lockable storage devices for pesticides in sri lanka that might assist in prevention of self-poisoning.self-poisoning with pesticides is a major reason for high suicide rates in rural areas of many developing countries. safer storage of pesticides may be one means of prevention. we have conducted a study to assess the acceptability and use of lockable boxes for storing pesticides in rural sri lanka.200919239714
surgical treatment of superior sulcus tumors: results and prognostic factors.this study aims to investigate the treatment modalities and factors influencing survival in surgically treated superior sulcus tumors.200919241311
microscopic pka analysis of glu286 in cytochrome c oxidase (rhodobacter sphaeroides): toward a calibrated molecular model.as stringent tests for the molecular model and computational protocol, microscopic pk(a) calculations are performed for the key residue, glu286, in cytochrome c oxidase (cco) using a combined quantum mechanical/molecular mechanical (qm/mm) potential and a thermodynamic integration protocol. the impact of the number of water molecules in the hydrophobic cavity and protonation state of several key residues (e.g., his334, cu(b)-bound water, and prd(a3)) on the computed microscopic pk(a) values of g ...200919243111
palladium-103 ophthalmic plaque radiation therapy for choroidal melanoma: 400 treated patients.to describe 18 years of experience with palladium-103 ((103)pd) ophthalmic plaque brachytherapy.200919243829
locally advanced rectal cancer: mr imaging in prediction of response after preoperative chemotherapy and radiation therapy.to prospectively differentiate, at magnetic resonance (mr) imaging, patients with locally advanced nonmucinous rectal cancer who will respond to long-course chemotherapy and radiation therapy (crt) from those who will not respond, with histopathologic results as the reference standard.200919244043
functional complementation of uvsx and uvsy mutations in the mediation of t4 homologous recombination.bacteriophage t4 homologous recombination events are promoted by presynaptic filaments of uvsx recombinase bound to single-stranded dna (ssdna). uvsy, the phage recombination mediator protein, promotes filament assembly in a concentration-dependent manner, stimulating uvsx at stoichiometric concentrations but inhibiting at higher concentrations. recent work demonstrated that uvsx-h195q/a mutants exhibit decreased ssdna-binding affinity and altered enzymatic properties. here, we show that unlike ...200919244311
advanced oral tongue cancer in a 22-year-old pregnant woman.oral squamous cell carcinoma generally occurs in older men and is associated mostly with tobacco and alcohol as the primary risk factors. the incidence of oral squamous cell carcinoma in younger patients has increased. we report a case of a young woman, 25 weeks pregnant, who presented with stage iv carcinoma of the oral tongue.200919244959
[postoperative analgesia with fentanyl combined with flurbiprofen axetil following gynecologic surgery for turnor].a to observe the analgesic effect of fentanyl combined with flurbiprofen axetil for postoperative analgesia after gynecologic surgery.200919246310
cortactin, fascin, and survivin expression associated with clinicopathological parameters in esophageal squamous cell carcinoma.cortactin, fascin, and survivin have been documented in several human cancers and play important roles in tumor progression. we collected 57 surgical specimens, including esophageal squamous cell carcinomas (sqcc; 7 well-differentiated, 15 moderately differentiated, and 24 poorly differentiated), 3 dysplasias, and 8 normal esophageal tissues. tissue microarrays were constructed and the immunostaining scores for cortactin, fascin, and survivin were assessed. in 46 sqcc specimens, we examined the ...200919207554
effects of combined interferon alpha and ribavirin therapy on thyroid functions in patients with chronic hepatitis c.to determine the frequency of thyroid dysfunction in patients of chronic hepatitis c during treatment with interferon alpha-2b and ribavirin therapy.200919208310
accelerated postnatal growth increases lipogenic gene expression and adipocyte size in low-birth weight mice.to characterize the hormonal milieu and adipose gene expression in response to catch-up growth (cug), a growth pattern associated with obesity and diabetes risk, in a mouse model of low birth weight (lbw).200919208909
aluminium siting in the zsm-5 framework by combination of high resolution 27al nmr and dft/mm calculations.the al siting in the zsm-5 zeolite was investigated by (27)al 3q mas nmr spectroscopy and qm/mm calculations. it was found that the occupation of the framework t-sites by al and the concentration of al in these t-sites are neither random nor controlled by a simple rule. they both depend on the conditions of the zeolite synthesis. at least 12 out of the 24 distinguishable framework t-sites of zsm-5 are occupied by al in the set of the investigated zeolite samples. a partial identification of the ...200919209368
identification of high-risk stage ii colorectal tumors by combined analysis of the ndrg1 gene expression and the depth of tumor invasion.experiments on cancer cell lines and animal models indicated that alteration in expression of n-myc down-regulated gene 1 (ndrg1) is associated with development of colon cancer. however, few clinical data are available to assess the role of ndrg1 in progression of human colorectal cancer. this study was undertaken to reveal the prognostic and predictive usefulness of ndrg1 expression determination in colorectal cancer.200919259744
the revised tnm staging system for lung cancer.the international staging committee (isc) of the international association for the study of lung cancer (iaslc) collected 68,463 patients with nonsmall cell lung cancer and 13,032 patients with small cell lung cancer, registered or diagnosed from 1990 to 2000, whose records had adequate information for analyzing the tumor, node, metastasis (tnm) classification. the t, n, and m descriptors were analyzed, and recommendations for changes in the seventh edition of the tnm classification were propose ...200919262443
inflammatory response after cardiopulmonary bypass: a randomized comparison between conventional hemofiltration and steroids.recent reports have shown anti-inflammatory effects with conventional hemofiltration (cuf) in patients undergoing cardiopulmonary bypass (cpb). the aim of this study was to evaluate the immunological and the hemodynamic response to cuf or metilprednisolone in patients undergoing coronary artery bypass grafting.200919262457
[effect of ginkgo biloba extract on plasma vascular endothelial growth factor during peri-operative period of cardiac surgery].to investigate the effect and clinical value of ginkgo biloba extract (ginaton) on the plasma vascular endothelial growth factor (vegf) in patients during peri-operative period of cardiac surgery.200919338151
t4 phages against escherichia coli diarrhea: potential and problems.a combination of in vitro and in vivo experiments with comparative phage genomics was used for the rational design of a phage cocktail against e. coli diarrhea. orally applied t4 coliphages representing three different subgroups (t4-, rb49- and js98-like phages) had no negative impact on the murine gut microbiota. t4 phages were found with high titers in the cecum and colon and lower titers in the small intestine, but were not detected in the blood, liver or spleen. no adverse effects were obser ...200919339031
three-dimensional classification of thoracic scoliotic curves.three-dimensional (3d) characterization of the thoracic scoliotic spine (cross-sectional study).200919127167
clinical efficacy of leukofiltration on cardiopulmonary bypass related inflammatory response: fact or foe?the powerful precept of preoperative risk assessment has been applied to compare the efficacy of leukofiltration techniques for high-risk cohorts with the documentation of broad indicators of systemic inflammation.200919266265
clinicopathologic features of renal cell carcinoma in young adults: a comparison study with renal cell carcinoma in older patients.to evaluate the clinicopathologic features of renal cell carcinoma in younger adults (</=40 years), we retrospectively reviewed 838 consecutive cases of renal cell carcinoma (rcc) occurred in a single tertiary hospital. forty-four 44 (5.2%) cases occurred in the young adult group (24 to 40 years of age). clinicopathologic features including tumor size, stage, histologic subtype, lymph node and distant metastasis, and overall survival were compared with that of cases occurred in older age group ( ...200919294008
value of frozen section analysis of enlarged lymph nodes during radical nephrectomy for renal cell carcinoma.to avoid unnecessary lymphadenectomy for renal cell cancer (rcc) in patients with retroperitoneal enlarged lymph nodes (elns).200919362343
rare involvement of submandibular gland by oral squamous cell carcinoma.the true involvement of the submandibular gland in primary oral cancer seems quite uncommon.200919360744
thyroxine autoantibody interference is an uncommon cause of inappropriate tsh secretion using the immulite 2000 assay.inappropriate tsh secretion, as defined by an elevated free thyroxine (ft4) and non-suppressed thyrotropin (tsh) result, can be caused by acute illness, medications, tsh secreting tumours, thyroid hormone resistance or immunoassay interference including thyroid hormone autoantibody interference. t4 autoantibody (t4aab) prevalence has not been determined. we determined the prevalence of inappropriate tsh secretion using the immulite 2000 assay and the prevalence of t4aab within this subgroup.200919361452
nmr structure of a monomeric intermediate on the evolutionarily optimized assembly pathway of a small trimerization domain.efficient formation of specific intermolecular interactions is essential for self-assembly of biological structures. the foldon domain is an evolutionarily optimized trimerization module required for assembly of the large, trimeric structural protein fibritin from phage t4. monomers consisting of the 27 amino acids comprising a single foldon domain subunit spontaneously form a natively folded trimer. during assembly of the foldon domain, a monomeric intermediate is formed on the submillisecond t ...200919361528
identification of orthodontic patients at risk of severe apical root resorption.current information suggests that the major variation in orthodontic root resorption can be explained by differences in individual predisposition. our aim was therefore to test the predictive value of the amount of maxillary incisor resorption about 6 and 12 months after bracket placement for the resorption at appliance removal.200919361730
efficacy and safety of treating t4 oral cavity tumors with primary chemoradiotherapy.patients with t4 oral cavity (oc) tumors are often treated with surgery followed by adjuvant chemoradiotherapy (crt).200919340870
lipoplex nanostructures reveal a general self-organization of nucleic acids.lipid and plasmid dna complexes (lx) were designed for gene transfer and were studied comprehensibly to elucidate their formation and ultrastructure.200919344746
thyroid hormone status in malnourished children.this study sought to find out the correlation between serum concentration of thyroid hormones and grade of malnutrition among 60 children between 1-5 year having pem. with increasing severity of malnutrition, the serum concentration of t3 and t4 progressively decreased and that of serum tsh increased.200919346578
development and characterization of an overtraining animal model.development of an endurance training-overtraining protocol for wistar rats that includes increased workload and is characterized by analyses of performance and biomarkers.200919346970
paraplegia after chest wall resection for primitive neuroectodermal tumor.spinal cord complications are rarely encountered in elective pediatric surgery. we present a patient who became paraplegic after resection of a chest wall tumor. after neoadjuvant radio- and chemotherapy, a 10-year-old girl with primitive neuroectodermal tumor (pnet) of the right chest underwent a right chest wall resection. ribs #3, 4, 5, and 6 were resected en masse with a pulmonary wedge resection of right upper and middle lobes. to obtain clear margins, the ribs were disarticulated from the ...200919349002
permanent and transient congenital hypothyroidism in isfahan-iran.to determine the prevalence of permanent and transient congenital hypothyroidism (ch) in isfahan, iran.200919349525
minimally invasive posterolateral thoracic corpectomy: cadaveric feasibility study and report of four clinical cases.to demonstrate the feasibility of and initial clinical experience with a novel minimally invasive posterolateral thoracic corpectomy technique.200919349833
euthyroid pretibial myxedema and emo syndrome.emo syndrome is a rare extrathyroid syndrome, seen in only 1% of patients affected by extrathyroid complications of graves' disease. a 73-year-old woman presented with a 1-year history of asymptomatic local swellings on her legs and feet. physical examination revealed moderate proptosis and multiple, firm subcutaneous nodules of 1 to 5 cm in diameter located on the anterior and medial aspects of the shins and on feet. the patient had a history of bilateral knee arthroplasty 5 years ago. histopat ...200919350184
bladder preservation therapy conducted by intra-arterial chemotherapy and radiotherapy for muscle invasive bladder cancer.a previous paper reported favorable results of intra-arterial chemotherapy in combination with radiotherapy for muscle-invasive bladder cancer. the current study will update those results.200919351661
carcinoma of the nasal cavity and paranasal sinuses.to determine the outcomes after radiotherapy (rt) alone or combined with surgery at the university of florida for patients with carcinomas of the nasal cavity and paranasal sinuses.200919358246
does vocal cord fixation preclude nonsurgical management of laryngeal cancer?to determine whether vocal cord fixation precludes nonsurgical management of t3/t4 laryngeal carcinoma.200919358250
the genome and proteome of a virulent escherichia coli o157:h7 bacteriophage closely resembling salmonella phage felix o1.based upon whole genome and proteome analysis, escherichia coli o157:h7-specific bacteriophage (phage) wv8 belongs to the new myoviral genus, "the felix o1-like viruses" along with salmonella phage felix o1 and erwinia amylovora phage phiea21-4. the genome characteristics of phage wv8 (size 88.49 kb, mol%g+c 38.9, 138 orfs, 23 trnas) are very similar to those of phage felix o1 (86.16 kb, 39.0 mol%g+c, 131 orfs and 22 trnas) and, indeed most of the proteins have their closest homologs within feli ...200919379502
[construction, identification of human b7.1 eukaryotic expressing vector and its expression on leukemic cells].the aim of study was to investigate the construction, identification and expression of human b7.1 (cd80) eukaryotic expressing vector on hl-60 cells. b7.1 gene was subcloned from the cloning vector using pcr. the pcr products and eukaryotic expressing vector (phook) both were separately digested with apai, sali and were ligated using t4 dna ligase. the ligased products were transduced into dh-5alpha. b7.1 gene containing clones was selected by digestion with apai and sali, and were further confi ...200919379586
positron emission tomography-computed tomography in predicting locoregional invasion in esophageal squamous cell carcinoma.in order to clarify the role of positron emission tomography-computed tomography (pet/ct) in thoracic esophageal squamous cell carcinoma we investigated its value in predicting locoregional invasion.200919379906
pneumocephalus after resection of a lung cancer with posterior chest wall involvement.pneumocephalus after resection of intrathoracic tumors is an extremely rare event. a delayed presentation of iatrogenic subarachnoid pleural fistula resulted in symptomatic pneumocephalus after resection of a locally recurrent t4n0 nonsmall cell lung cancer involving the chest wall, t2 to t4 rib heads, and intercostal vertebral foramina. progressive neurologic decline was noted 3 weeks after discharge. computed tomography of the head and chest confirmed the presence of an apical pleural space, t ...200919379924
evaluation of the new tnm staging system proposed by the international association for the study of lung cancer at a single institution.the seventh tnm classification of malignant tumours will be published in 2009. the international association for the study of lung cancer has proposed a revision of the current pathologic staging system. we illustrated the effects of this new system and pointed out potential problems using a retrospective study of surgical cases of non-small cell lung cancer at our institution.200919379988
screening for dysfunction to promote multidisciplinary intervention by using the university of washington quality of life questionnaire.to investigate the potential of the university of washington quality of life questionnaire (uw-qol) in routine clinics as a quick screening tool for possible dysfunction in patients after treatment of head and neck cancer.200919380359
acceptance of major surgical procedures and quality of life among long-term survivors of advanced head and neck cancer.to evaluate the acceptance of major surgical procedures and quality of life among long-term survivors of advanced head and neck cancer treated at a single institution.200919380360
robot-assisted surgery for upper aerodigestive tract neoplasms.to assess the feasibility and safety of performing robot-assisted resections of head and neck tumors, and to predict which variables lead to successful robot-assisted resection and better functional outcome.200919380364
crystal structure of the beta beta alpha-me type ii restriction endonuclease hpy99i with target dna.the beta beta alpha-me restriction endonuclease (rease) hpy99i recognizes the cgwcg target sequence and cleaves it with unusual stagger (five nucleotide 5'-recessed ends). here we present the crystal structure of the specific complex of the dimeric enzyme with dna. the hpy99i protomer consists of an antiparallel beta-barrel and two beta 4 alpha 2 repeats. each repeat coordinates a structural zinc ion with four cysteine thiolates in two cxxc motifs. the beta beta alpha-me region of the second bet ...200919380375
preoperative t staging of urinary bladder cancer: does diffusion-weighted mri have supplementary value?the objective of our study was to evaluate whether diffusion-weighted mri has supplementary value in the preoperative t staging of urinary bladder cancer.200919380561
[gastrojejunostomy for irresectable gastric cancer with pyloric stenosis-new role of surgery in the era of s-1].we report a patient with an advanced gastric cancer complicated by pyloric stenosis who was effectively treated by s-1 mono-therapy after gastrojejunostomy. a 62-year-old man consulted a general practitioner for abdominal pain and anorexia. gastric roentgenography and upper gastrointestinal endoscopy showed gastric cancer(borrmann type 3) with pyloric stenosis. he was referred to our department. he underwent laparotomy, which revealed a t4 tumor invading the pancreas head, but neither liver nor ...200919381039
treatment and outcome of advanced external auditory canal and middle ear squamous cell carcinoma.this is a retrospective study to evaluate the outcomes and complications of combined treatment, surgery with or without adjunctive intraoperative radiotherapy, of locally advanced temporal bone squamous cell carcinoma. a series of 17 patients with locally advanced squamous cell carcinoma of the temporal bone were treated between september 2002 and february 2007. eleven patients had primary tumors, and 6 patients had recurrences. according to the university of pittsburgh staging system, 5 patient ...200919381105
effects of thyroid status on arginine vasotocin receptor vt2r expression and adrenal function in osmotically stimulated domestic fowl.the role of thyroid hormones in the regulation of adrenal function during stress has been documented in mammals, but only limited reports are available in avian species. the present study was undertaken to analyze the effect of hyper- or hypothyroidism on the adrenal activity under control (hydrated) and osmotically stressed (water deprived, wd) conditions, with special emphasis on the expression of arginine vasotocin receptor vt2 (vt2r) in pituitary corticotrophs. chickens were made hyper- or h ...200919381646
the zn center of the anaerobic ribonucleotide reductase from e. coli.strict and facultative anaerobes depend on a class iii ribonucleotide reductase for their growth. these enzymes are the sole cellular catalysts for de novo biosynthesis of the deoxyribonucleotides needed for dna chain elongation and repair. in its active form, the class iii ribonucleotide reductase from escherichia coli contains a free radical located on the g681 residue which is essential for the activation of the ribonucleotide substrate toward its reduction. the 3d structure of the homologous ...200919381696
methods to study transcription-coupled repair in chromatin.transcription-coupled repair (tcr) is a sub-pathway of nucleotide excision repair that allows for the enhanced repair of the transcribed strand of active genes. a classical method to study dna repair in vivo consists in the molecular analysis of uv-induced dna damages at specific loci. cells are irradiated with a defined dose of uv light leading to the formation of dna lesions and incubated in the dark to allow repair. about 90% of the photoproducts consist of cyclobutane pyrimidine dimers, whic ...200919381941
does the circadian rhythm of melatonin affect ischemia-reperfusion injury after coronary artery bypass grafting?melatonin is a potent scavenger of free radicals and an antioxidant. we studied the relationship between the protective effect of melatonin against ischemia-reperfusion injury (iri) during cardiopulmonary bypass, the plasma level of melatonin, and the time of surgery.200919383595
evaluation at atomic resolution of the role of strain in destabilizing the temperature-sensitive t4 lysozyme mutant arg 96 --> his.mutant r96h is a classic temperature-sensitive mutant of bacteriophage t4 lysozyme. it was in fact the first variant of the protein to be characterized structurally. subsequently, it has been studied extensively by a variety of experimental and computational techniques, but the reasons for the loss of stability of the mutant protein remain controversial. in the crystallographic refinement of the mutant structure at 1.9 a resolution one of the bond angles at the site of substitution appeared to b ...200919384984
contributions of all 20 amino acids at site 96 to the stability and structure of t4 lysozyme.to try to resolve the loss of stability in the temperature-sensitive mutant of t4 lysozyme, arg 96 --> his, all of the remaining 18 naturally occurring amino acids were substituted at site 96. also, in response to suggestions that the charged residues lys85 and asp89, which are 5-8 a away, may have important effects, each of these amino acids was replaced with alanine. crystal structures were determined for many of the variants. with the exception of the tryptophan and valine mutants r96w and r9 ...200919384988
structural origin of weakly ordered nitroxide motion in spin-labeled proteins.a disulfide-linked nitroxide side chain (r1) used in site-directed spin labeling of proteins often exhibits an epr spectrum characteristic of a weakly ordered z-axis anisotropic motion at topographically diverse surface sites, including those on helices, loops and edge strands of beta-sheets. to elucidate the origin of this motion, the first crystal structures of r1 that display simple z-axis anisotropic motion at solvent-exposed helical sites (131 and 151) and a loop site (82) in t4 lysozyme ha ...200919384990
validation of spinal motion with the spine reposition sense device.a sagittal plane spine reposition sense device (srsd) has been developed. two questions were addressed with this study concerning the new srsd: 1) whether spine movement was occurring with the methodology, and 2) where movement was taking place.200919386126
treatment of nasopharyngeal carcinoma using intensity-modulated radiotherapy-the national cancer centre singapore experience.the aim of this study was to determine the efficacy and acute toxicity of our early experience with treating nasopharyngeal carcinoma (npc) patients with intensity-modulated radiotherapy (imrt).200919386431
effect of fluoride varnish or nail polish on the superficial roughness of amalgam restorations.the aim of this in vitro study was to evaluate the superficial roughness of amalgam specimens made with velvalloy and permite alloys, with superficial protection (nail polish or varnish-fluorniz) and without protection, immersed in artificial saliva or coke.200919369921
[transthyretin: it's miracle function and pathogenesis].transthyretin (ttr) was previously called prealbumin because the band it formed on agarose gel electrophoresis at ph 8.6 was at the prealbumin position. however, it has been well documented that ttr of rodents does not show a prealbumin position on electrophoresis. now, its name describes its function, binding to retinol binding protein (rbp) and t4. the serum concentration of the protein is 20-40 mg/dl, and ttr forms a tetramer. the plasma half life of the protein is 1.9 days. ttr is synthesize ...200919363993
spinal cord infarction after operative stabilisation of the thoracic spine in a patient with tuberculous spondylodiscitis and sickle cell trait.case report.200919365238
diameter, length, and direction of pedicle screws for scoliotic spine: analysis by multiplanar reconstruction of computed tomography.a morphometric study of thoracic and lumbar spine in scoliosis.200919365247
the bacteriophage t4 asia protein contacts the beta-flap domain of rna polymerase.to initiate transcription from specific promoters, the bacterial rna polymerase (rnap) core enzyme must associate with the initiation factor sigma, which contains determinants that allow sequence-specific interactions with promoter dna. most bacteria contain several sigma factors, each of which directs recognition of a distinct set of promoters. a large and diverse family of proteins known as "anti-sigma factors" regulates promoter utilization by targeting specific sigma factors. the founding me ...200919366670
incidence and patterns of isolated brain failure in stage iii non-small-cell lung cancer treated with concurrent chemoradiation therapy.the incidence and patterns of isolated brain failure was examined in patients with stage iii non-small-cell lung cancer (nsclc) treated with concurrent chemoradiation (ccrt).200919373528
impact of reconstructive microsurgery in patients with advanced oral cavity cancers.our goal was to determine the impact of reconstructive microsurgery on the treatment of advanced oral cavity cancers.200919373778
rolling-circle amplification: unshared advantages in mirna detection.roll with it: the quantitative analysis of specific mirnas from biological samples is very likely to revolutionize diagnostics of human disease. a novel method for mirna analysis employing rolling-circle amplification (rca) can homogeneously detect mirna, even at concentrations as low as 10 fm. the use of t4 rna ligase 2 (t4 rnl2) at elevated temperatures enables very good discrimination of mirnas differing by a single nucleotide.200919373796
outcomes of clinical t4m0 extra-peritoneal rectal cancer treated with preoperative radiochemotherapy and surgery: a prospective evaluation of a single institutional experience.our objective was evaluate the outcome of primary clinical t4m0 extraperitoneal rectal cancer treated by neoadjuvant radiochemotherapy. prognosis of clinical t4 rectal cancer is poor. preoperative chemoradiation therapy may be beneficial. the results obtained are unclear due to lack of objective and strictly applied staging methods.200919375606
clinical and biomaterial evaluation of hyaluronan-based heparin-bonded extracorporeal circuits with reduced versus full systemic anticoagulation in reoperation for coronary revascularization.this prospective randomized study compares full and reduced heparinization on novel hyaluronan-based heparin-bonded circuits vs. uncoated controls under challenging clinical setting including biomaterial evaluation.200919377380
[clinical study on monitoring right ventricular end-diastolic volume in volume management during orthotopic liver transplantation].to explore the feasibility of guiding the volume management during orthotopic liver transplantation by right ventricular end-diastolic volume index(rvedvi).200919377628
ipsilateral testicular necrosis and atrophy after 1,080-degree torsion of the spermatic cord in rats.to assess the incidence of testicular necrosis/atrophy immediately after 1 to 4 hours of 1,080-degree torsion of the spermatic cord, and 60 days after detorsion of the spermatic cord.200919377780
priming with rocuronium to accelerate the onset time of cisatracurium during intubation.the priming technique, in which a small dose of nondepolarizing muscle relaxant is administered 3-6 minutes before giving the intubation dose, can speed up the onset of muscle relaxation in patients with paralysis during intubation. we investigated the priming technique and compared 2 different priming agents (rocuronium and cisatracurium) at a priming time of 3 minutes and its effect on decreasing the onset time of cisatracurium.200919181592
induction chemoradiotherapy facilitates radical resection of t4 non-small cell lung cancer invading the spine.we evaluated the outcome, long-term results, and factors affecting outcome of induction chemoradiotherapy followed by surgical resection for t4 non-small cell lung cancer invading the spine.200919185167
isolation and genotyping of potentially pathogenic acanthamoeba strains from dust sources in iran.opportunistic free-living amoebae of the acanthamoeba genus present genotypes/strains that are causative agents of acanthamoeba keratitis (ak), with genotype t4 related to most ak cases worldwide. in order to prove that exposure to dirt and dust could be an important risk factor for contact lens wearers in iran, 13 strains isolated from dust samples in this area were classified at the genotype level. results revealed the presence of t4, t5 and t11 genotypes within these samples. to our knowledge ...200919185896
uva generates pyrimidine dimers in dna directly.there is increasing evidence that uva radiation, which makes up approximately 95% of the solar uv light reaching the earth's surface and is also commonly used for cosmetic purposes, is genotoxic. however, in contrast to uvc and uvb, the mechanisms by which uva produces various dna lesions are still unclear. in addition, the relative amounts of various types of uva lesions and their mutagenic significance are also a subject of debate. here, we exploit atomic force microscopy (afm) imaging of indi ...200919186150
thoracic epidural analgesia in post-thoracotomy patients: comparison of three different concentrations of levobupivacaine and sufentanil.relative effects of dosage, volume and concentration of local anaesthetics used for postoperative thoracic epidural analgesia are still under debate. in this randomized, prospective, double-blinded study, we evaluated the incidence of side-effects such as changes in arterial pressure, postoperative nausea, vomiting, and pruritus in patients admitted for thoracic surgery during continuous thoracic epidural infusion using levobupivacaine and sufentanil mixture in three different volumes.200919189982
developmental exposure to chlorpyrifos induces alterations in thyroid and thyroid hormone levels without other toxicity signs in cd-1 mice.organophosphorus insecticides, as chlorpyrifos (cpf), are widely used in agriculture and against household pests; these compounds receive an increasing consideration as potential endocrine disrupters. the aim of the present study was to examine the potential short- and long-term effects of cpf on thyroid and adrenal glands in cd1 mice following exposure at dose levels not inducing brain acetyl cholinesterase (ache) inhibition, during gestational and/or postnatal vulnerable phases. pregnant dams ...200919190125
multifrequency electron spin resonance spectra of a spin-labeled protein calculated from molecular dynamics simulations.multifrequency electron spin resonance (esr) spectra provide a wealth of structural and dynamic information about the local environment of the spin label and, indirectly, about the spin-labeled protein. relating the features of the observed spectra to the underlying molecular motions and interactions is, however, challenging. to make progress toward a rigorous interpretation of esr spectra, we perform extensive molecular dynamics (md) simulations of fully solvated t4 lysozyme, labeled with the s ...200919191603
tsh-based protocol, tablet instability, and absorption effects on l-t4 bioequivalence.fda guidance for pharmacokinetic (pk) testing of levothyroxine (l-t(4)) for interbrand bioequivalence has evolved recently. concerns remain about efficacy and safety of the current protocol, based on pk analysis following supraphysiological l-t(4) dosing in euthyroid volunteers, and recent recalls due to intrabrand manufacturing problems also suggest need for further refinement. we examine these interrelated issues quantitatively, using simulated what-if scenarios testing efficacy of a tsh-based ...200919191742
evaluation of mood profiles during malaria chemoprophylaxis: a randomized, double-blind, four-arm study.to objectively compare the mood profiles of users of malaria chemoprophylaxis regimens (atovaquone-proguanil, chloroquine-proguanil, doxycycline, or mefloquine) in a group of nonimmune tourists to sub-saharan africa.200919192127
[thyroid disorders and childhood rheumatic diseases].the relationship between thyroid dysfunction and autoimmune diseases has mainly been described in adults. the aim of this study was to analyse the prevalence and characteristics of thyroid abnormalities in children with rheumatic diseases.200919174120
review of 120 anal cancer patients.chemoradiotherapy is the mainstay of treatment for the majority of patients with anal cancer, with abdominoperineal resection reserved for salvage. the purpose of this study was to evaluate our results after radiotherapy with or without chemotherapy, and/or surgery in terms of overall survival and colostomy free survival in patients with anal cancer.200919175651
clinical evaluation of a novel liquid formulation of l-thyroxine for once daily treatment of dogs with hypothyroidism.a liquid solution of levothyroxine (l-t4) is available for treatment of canine hypothyroidism.200919175719
phase 3 randomized trial on larynx preservation comparing sequential vs alternating chemotherapy and radiotherapy.both induction chemotherapy followed by irradiation and concurrent chemotherapy and radiotherapy have been reported as valuable alternatives to total laryngectomy in patients with advanced larynx or hypopharynx cancer. we report results of the randomized phase 3 trial 24954 from the european organization for research and treatment of cancer.200919176454
free-solution label-free detection of alpha-crystallin chaperone interactions by back-scattering interferometry.we report the quantitative, label-free analysis of protein-protein interactions in free solution within picoliter volumes using backscatter interferometry (bsi). changes in the refractive index are measured for solutions introduced on a pdms microchip allowing determination of forward and reverse rate constants for two-mode binding. time-dependent bsi traces are directly fit using a global analysis approach to characterize the interaction of the small heat-shock protein alpha-crystallin with two ...200919178288
accuracy of impression techniques for implants. part 2 - comparison of splinting techniques.the aim of this study was to compare splinting techniques for impression copings of osseointegrated implants with different angulations.200919178624
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