Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| in vitro antifungal activity of can-296: a naturally occurring complex carbohydrate. | the in vitro activity of a naturally occurring complex carbohydrate, can-296, was evaluated by testing 132 clinical and atcc isolates of yeast and aspergillus fumigatus, many of which were azole-resistant. the in vitro susceptibility tests were performed by standardized broth micro- and macrodilution methods and results were compared with those obtained for amphotericin b, fluconazole, ketoconazole, flucytosine and the pneumocandin l-733,560. all tested candida species showed highly uniform susc ... | 1997 | 9592567 |
| a comparison of sampling methods for airborne fungal spores during an outbreak of aspergillosis in the forest aviary of the north carolina zoological park. | an outbreak of aspergillosis with the death of six birds in the north carolina zoological park r. j. reynolds forest aviary in the spring of 1993 led to an investigation of the concentration of aspergillus fumigatus spores in the air. no aspergillus sp. was found in the facility through use of the drop plate method (gravitometric sampling) along with swab-sampling of selected surfaces within the exhibit and plating of food samples and nesting material onto petri dishes of nutrient media. a numbe ... | 1997 | 9523640 |
| prevalence of otomycosis in malnourished children in edo state, nigeria. | out of the total number at 200 suspected cases of otomycoses consisting of 40 malnourished and 160 apparently healthy children examined in this study between the months of july and august in edo state, 64 cases (32%) were identified to be of fungal aetiology on the basis of positive culture and careful microscopic examination. the state at protein energy malnourishment was deterwined using physicians' comments in their case files. the fungal agents isolated were aspergillus niger 28 (43.8%); a. ... | 1997 | 9646512 |
| [fungal infection of human organs by resistant melanin-synthesizing species is one of the pathogenic factors and one of the real consequences of the accident at chernobyl power plant]. | free radical melanin centers have been detected in the cell concentrate of bronchoalveolar lavage (bal) of liquidator of chernobyl npp accident. to identify the nature of these centers the epr technique and the fluorescent technique were used to study bal of liquidators with lung chronic pathology, their blood, blood components as well as model melanin- and lipofuscin-containing systems: synthetic dopa-melanin, human melanosome, human lipofuscin, human melanolipofuscin. i besides that we have in ... | 1997 | 9599625 |
| detection of aspergillus ribosomal rna using biotinylated oligonucleotide probes. | aspergillosis continues to be a devastating disease entity that results in significant mortality in immunosuppressed patients. rapid diagnosis is often required to initiate appropriate therapy. although the histopathologist may be able to visualize fungal organisms in tissue specimens, the histology of aspergillus species may overlap with a variety of fungi, so diagnosis often relies on fungal cultures that can take weeks to complete. recently, an in situ hybridization assay targeting aspergillu ... | 1997 | 9458383 |
| applicability of random primer r143 for determination of aspergillus fumigatus dna. | the specificity of random primer r143 for aspergillus fumigatus dna was determined in order to test its usefulness in establishing the presence of a. fumigatus dna in fungal cultures. when pcr reaction products of these cultures were compared with those of 21 other bacterial and fungal dna samples, r143 proved to produce a 1346 bp band with only a. fumigatus. this band has been sequenced completely and the ecori restriction site was used for subsequent confirmation of pcr products. the specifici ... | 1997 | 9467106 |
| morphological characteristics of aspergillus fumigatus strains isolated from patient samples. | the aspergillus fumigatus group splits into the anamorphic a. fumigatus series and the teleomorphic neosartorya fischeri series. the species a. fumigatus fresenius is the most common species within the a. fumigatus series. forty-seven clinical isolates out of the a. fumigatus group were obtained from patients with diseases such as localized and invasive aspergilloses or allergic disorders due to aspergillus and were mainly isolated from respiratory secretions. isolates were studied concerning ma ... | 1997 | 9470420 |
| in vitro interactions between histoplasma capsulatum var. duboisii and other fungi. | histoplasma capsulatum var. duboisii has only recently been shown to occur naturally in soil admixed with bat guano in a bat cave. we report our observations on the in vitro interaction of histoplasma capsulatum var. duboisii with other fungi recovered from the same habitat or other sources by employing co-culture technique and by investigating antibiosis between h. capsulatum var. duboisii and other fungi. the range of interactions varied from total overgrowth of histoplasma by aspergillus fumi ... | 1997 | 9476515 |
| articular aspergillosis: two case reports and review of the literature. | we report two cases of septic arthritis of the knee caused by aspergillus: one by aspergillus terreus in a cirrhotic patient and the other by aspergillus fumigatus after vascular graft infection. the recovery of these organisms in synovial fluid should be considered as pathogenic, particularly in immunocompromised hosts. the pathogenic mechanism is discussed and the literature is reviewed. | 1997 | 9448597 |
| [cystic fibrosis in adults--clinical aspects]. | nineteen patients with cystic fibrosis were seen in the i department of tuberculosis and lung diseases during 3.5 years. there were 12 (63%) female, and 7 male, aged from 16 to 35 years (mean 23.2). most patients were diagnosed in childhood, but 4 were diagnosed in their early adulthood. the diagnosis was confirmed by positive chloride sweat test in all cases. molecular dna analyses were performed in 16 cases. in 9 (56%) cases two mutations in the cftr gene were identified. in 5 cases one mutati ... | 1997 | 9489415 |
| [invasive pulmonary aspergillosis caused by aspergillus ochraceus]. | invasive pulmonary aspergillosis (ipa) is a frequent complication in patients with severe neutropenia resulting from cytotoxic chemotherapy. in europe, aspergillus fumigatus is most common pathogen of ipa. in our case, ipa was recognised in 54 year old female suffering from acute lymphoblastic leukemia with pancytopenia. the patient developed severe granulocytopenia during chemotherapy. chest radiograph revealed progressive bilateral infiltrations with cavitation. in sputum culture aspergillus o ... | 1997 | 9489424 |
| [endogenous aspergillus endophthalmitis in aids]. | we present the course of an endogenous aspergillus endophthalmitis in a 38-year-old hiv-positive man. history and clinical signs: the first examination because of a sudden visual loss in the right eye showed a central subretinal infiltration, peripheral vascular sheeting and retinal hemorrhages. | 1997 | 9498193 |
| [profound mycoses in aids in abidjan (côte d'ivoire)]. | during 1995, among 1105 hiv patients explored in our department, 64 presented a deep fungic infection (5.8%). the yeast was searched for in cerebrospinal fluid, blood, urine, and bronchoalveolar aspiration. isolated germs were cryptococcus neoformans (95%), candida tropicalis (1 case), saccharomyces cerevisiae (1 case) et aspergillus fumigatus (1 case). results of treatment with amphotericin b were: recovery (9%), clinical success (11%), out of sight (14%), letality (66%), relapse (23%) and side ... | 1997 | 9507758 |
| [approach to nasal aspergillosis]. | 1997 | 9534815 | |
| chronic pulmonary histoplasmosis in brazil: report of two cases with cavitation diagnosed by transthoracic needle biopsy. | two cases of chronic pulmonary histoplasmosis are reported and other eleven cases, collected from brazilian literature, are commented. after being clinically cured, one of our patients presented an aspergillus fungus ball inside a cavitation in the wall of which h. capsulatum was disclosed. comments are also done on the diagnosis of the mycosis. | 1997 | 9661309 |
| [synthesis and antifungal activities of 1-(1-substitutedphenyl)-2-(1h-1,2,4-triazol or benztriazol-1-yl)-o-(substitutedbenzyl) ethanoximes]. | twenty-nine 1-(1-substituted phenyl)-2-(1h-1,2,4-triazol or benztriazol-1-yl)-o-(substituted benzyl) ethanoximes have been synthesized for the first time. results of preliminary biological test in vitro show that most compounds have antifungal activities against most fungi tested. the antifungal activities of compounds t1, t4, t6, t11, t12, b1, b3, b4 and b6 are better than or comparable to the activities of the lead compound oxiconazole against some fungi. | 1997 | 11243218 |
| influence of essential oil of hyssopus officinalis on the chemical composition of the walls of aspergillus fumigatus (fresenius). | the cell walls of the growing hyphae of aspergillus fumigatus (fresenius) cultured in the presence or absence of the essential oil of hyssopus officinalis were isolated and their chemical composition analysed. the presence of the essential oil led to a reduction in levels of neutral sugars, uronic acid and proteins, whereas amino sugars, lipids and phosphorus levels were increased. hplc analysis of the neutral sugars showed that they consisted mainly of glucose, mannose and galactose, while the ... | 1997 | 16333566 |
| periodontal microflora of hiv infected patients with periodontitis. | the aim of this study was to determine the microbial profile of periodontal lesions in hiv seropositive patients and to compare it with rapidly progressing periodontal lesions in systemically healthy patients. the subgingival microflora of 20 cdc ii, 20 cdc iii, 20 cdc iv/v and 20 systemically healthy patients with rapidly progressing periodontitis was examined. four sites with greatest probing depth in each patient were selected for microbiological sampling. the samples were cultured aerobicall ... | 1997 | 16887570 |
| immunochemical reactivity of aspergillus fumigatus antigens from different sources. | we have studied the immunochemical and biochemical differences in 12 aspergillus fumigatus strains isolated from different sources. the enzymatic activity of all these strains were studied by a rapid enzyme detection method (api-zym). one of 12 strains studied produced alkaline phosphatase, while two produced chymotrypsin, and three produced trypsin. by sds-page we studied proteins present in the antigen extracts from all 12 strains. several of the protein bands were unique and may be used to di ... | 1997 | 16854171 |
| immunoreactivity of antigen extracts of aspergillus fumigatus isolated from different sources. | allergic bronchopulmonary aspergillosis (abpa) is the result of hypersensitivity to aspergillus antigens in patients with long-standing atopic asthma. in the present study mycelial and culture filtrate antigens from aspergillus fumigatus cultures isolated from diverse sources were tested against sera of 10 abpa patients and 10 control individuals by an elisa methodology. the results indicate higher antibody reactivity against both antigens in the sera of abpa patients, while culture filtrate ant ... | 1997 | 16854172 |
| immunohistochemistry for the diagnosis of aspergillosis in turkey poults. | from each of two flocks (a and b) of poults comprising 14,100 females and 11,300 males, respectively, 15 poults were examined pathologically. poults of flock a had signs of neurological disturbances whereas birds from flock b showed respiratory symptoms. gross lesions were observed only in two poults from flock a in which minute circular areas of cerebral malaria were seen. histopathologically, the brain lesions contained fungal elements, and so did some of the pulmonary granulomas detected in t ... | 1997 | 18483886 |
| meningeal aspergillosis (a case report). | 1997 | 28769524 | |
| [aspergillosis: mechanisms of pathogenicity implicated and approach to laboratory diagnosis.]. | aspergillosis comprises a wide range of clinical conditions, of which the most serious is invasive aspergillosis, which particularly affects immunodeficient individuals. in the present work we present a review centred on three main aspects of this disease: 1) possibility of differentiation of strains of aspergillus fumigatus by phenotyphic and genotypic methods, 2) mechanisms of pathogenicity of this species and especially the relationships of elastase activity, and 3) laboratory diagnosis of as ... | 1998 | 17655396 |
| prevalence and in vitro antifungal susceptibility of candida spp isolated from clinical specimens in são paulo, brazil. | the specifity of gene encoding the ribotoxin protein in aspergillus fumigatus was determined by pcr amplification of a portion of the gene. all a. fumigatus strains studied showed the presence of amplifiable ribotoxin product, while none of the other fungal species, with the exception of aspergillus restrictus, showed this amplification product. hence, this method may be used for rapid and specific identification of a. fumigatus. | 1998 | 17655400 |
| functional genomics conference: from identifying proteins to faster drug discovery. march 10-11, 1998, washington dc, usa. | the massive effort to sequence the human, mouse, rat, nematode (caenorhabditis elegans), fruit fly (drosophila), zebra fish, yeast (saccharomyces cerevisiae), fungal (candida albicans and aspergillus fumigatus) and several bacterial genomes has produced a flood of sequence data. of the more than 100,000 human genes and thousands from other organisms, many partial sequences and several completed microbial genomes are available in both public and private databases. however, elucidation of function ... | 1998 | 15992025 |
| renal aspergillosis giving rise to obstructive uropathy and recurrent anuric renal failure. | a sixty-year-old previously healthy male patient presented with anuric renal failure of sudden onset. he was detected to have aspergillus fumigatus fungal balls in the renal pelvis, ureters and bladder which were removed and his renal function improved. he was treated with itraconazole and sent home. three weeks later he again presented with anuria and renal failure. he had recurrence of the obstruction with the same fungus. the fungal ball was removed, a double 'j' stenting was performed and he ... | 1998 | 10221171 |
| successful treatment of aspergillus fungaemia in two children with acute lymphoblastic leukaemia. | aspergillus species can cause life-threatening infection in immunocompromised children. pulmonary infections are the most common, and are usually acquired through inhalations of aspergillus spores in unfiltered air. some patients acquire invasive aspergillus infection from endogenous spread of colonized para-nasal sinuses. | 1998 | 9661945 |
| steroid-induced invasive aspergillosis with thyroid gland abscess and positive blood cultures. | 1998 | 9665309 | |
| the role of the aspergillus fumigatus area gene in invasive pulmonary aspergillosis. | the area gene of aspergillus nidulans is a positive-acting transcriptional factor required for the expression of genes involved in the utilization of a broad range of nitrogen sources other than ammonium and glutamine. we have investigated the role in pathogenesis of the corresponding gene (afarea) of aspergillus fumigatus, a causative agent of invasive pulmonary aspergillosis. stable and unstable afarea- strains were constructed and tested for altered virulence in mice on the basis of host surv ... | 1998 | 9669338 |
| dna damage by gliotoxin from aspergillus fumigatus. an occupational and environmental propagule: adduct detection as measured by 32p dna radiolabelling and two-dimensional thin-layer chromatography. | gliotoxin is produced by the fungus aspergillus fumigatus. aspergillus is widespread in the environment and this ubiquitous nature results in disease and co-carcinogenesis to be distributed world-wide. gliotoxin contains an epipolythiodioxopiperazine (etp) ring that is believed to be involved in redox reactions. the reactive oxygen species produced interact with dna to form hydroxylated and other altered dna products. to measure dna adduct formation, we used 32p radiolabelling and, after enzymat ... | 1998 | 9670759 |
| identification and cloning of two novel allergens from the lipophilic yeast, malassezia furfur. | two novel allergens, designated mal f 2 and mal f 3 according to the who/iuis allergen nomenclature subcommittee recommendation, were isolated from the lipophilic yeast malassezia furfur cell extracts and the genes coding for those were cloned. mal f 2 and mal f 3 had apparent molecular weights of 21 kda and 20 kda, respectively, on sds-page under reducing conditions. the identified cdna clone of mal f 2 encoded an open reading frame of 177 amino acid residues. fifty-one percent identity was fou ... | 1998 | 9675120 |
| tryprostatin a, a specific and novel inhibitor of microtubule assembly. | we have investigated the cell cycle inhibition mechanism and primary target of tryprostatin a (tps-a) purified from aspergillus fumigatus. tps-a inhibited cell cycle progression of asynchronously cultured 3y1 cells in the m phase in a dose- and time-dependent manner. in contrast, tps-b (the demethoxy analogue of tps-a) showed cell-cycle non-specific inhibition on cell growth even though it inhibited cell growth at lower concentrations than tps-a. tps-a treatment induced the reversible disruption ... | 1998 | 9677311 |
| a murine model of allergic rhinitis: studies on the role of ige in pathogenesis and analysis of the eosinophil influx elicited by allergen and eotaxin. | allergic rhinitis is a prevalent disease with significant morbidity. studies of its pathophysiology in human subjects have been limited. nasal biopsy specimens are difficult to obtain, and nasal secretions incompletely reflect the cellular and molecular events in the mucosa. ige-mediated mast cell activation and the elaboration of factors promoting eosinophil development and chemotaxis are likely to participate in pathogenesis. | 1998 | 9679849 |
| aspergillus fumigatus is the causative agent of hypersensitivity pneumonitis caused by esparto dust. | 1998 | 9679860 | |
| mammary and systemic aspergillosis in dairy sheep. | mammary aspergillosis was diagnosed in four flocks of dairy sheep, comprising a total of 1,750 ewes. these animals had been treated prophylactically by intramammary infusion with cloxacillin 5 months prior to lambing. mammary aspergillosis with concomitant spread to the regional lymph nodes was present in these flocks in a percentage ranging from 2% to 36.4% of treated sheep. pathologic, bacteriologic, and mycologic studies were performed in seven of the affected ewes. some of them also had lung ... | 1998 | 9684966 |
| in-vitro isolation and antifungal susceptibility of amphotericin b-resistant mutants of aspergillus fumigatus. | aspergillus fumigatus mutants resistant to amphotericin b were selected in the laboratory following uv irradiation. a total of 18 colonies (frequency 1.8 x 10(-7)) that grew in the presence of amphotericin b (8 mg/l and 16 mg/l) on peptone yeast extract glucose agar were tested for their susceptibility to amphotericin b, nystatin, azoles, and the echinocandin l-743872. ten of the 18 isolates showed an eight-fold rise in amphotericin b mic (4 mg/l) compared with the susceptible parent whereas the ... | 1998 | 9687099 |
| efficacy of mk-991 (l-743,872), a semisynthetic pneumocandin, in murine models of pneumocystis carinii. | in addition to its potent efficacy in animal models against candida sp., aspergillus fumigatus, and histoplasma capsulatum, the clinical candidate pneumocandin mk-991 (formerly l-743,872) was also extremely potent against pneumocystis carinii in models of immune-compromised animals. mk-991 was approximately 14 times more potent than the original natural product lead, pneumocandin b0. the 90% effective dose (ed90) of mk-991 for cyst clearance in the rat model for pneumocystis was 0.011 mg/kg of b ... | 1998 | 9687394 |
| evaluation of antifungal activity of an antifungal drug by in vitro simulation of in vivo pharmacokinetics of the drug against fungal hyphal growth. | an automatic drug concentration simulator (dcs) has been developed and its applicability has been demonstrated by in vitro simulation of the human plasma concentration-time curve of fluconazole (flcz) against hyphal growth of candida albicans and aspergillus fumigatus. the response of hyphal growth to flcz was continually monitored and analyzed using an automatic hyphal growth analyzing system (bio-cell tracer). the simulated concentration of flcz by dcs was confirmed by hplc. the dcs assay was ... | 1998 | 9694984 |
| complex chitinolytic system of aspergillus fumigatus. | native polyacrylamide gels incorporating a glycol chitin substrate were used to detect several chitinolytic enzymes in the culture filtrate and cell surface, wall and mixed membrane fractions of aspergillus fumigatus during the exponential phase of growth. much of the cellular chitinase activity did not bind to concanavalin a (con a) matrix and was heat-sensitive. in contrast, almost all chitinases secreted appeared to be heat-stable glycoproteins. the heavily glycosylated molecules, in a con a- ... | 1998 | 9697338 |
| microsatellite markers for typing aspergillus fumigatus isolates. | the use of microsatellites as highly polymorphic dna markers for the typing of isolates of aspergillus fumigatus was investigated. four ca repeats were selected by screening an a. fumigatus dna library with a (ca)10 oligonucleotide. primers flanking these ca repeats were designed to amplify each locus. one primer of each pair was labeled with a fluorophore, and the pcr products were analyzed with an automatic sequencer and the genescan software. for each primer set and for a given isolate, one b ... | 1998 | 9705365 |
| interactive mortality factors in common loons from maritime canada. | between august 1992 and november 1995, 31 moribund or dead common loons (gavia immer) found in the three maritime provinces of canada (new brunswick, nova scotia, prince edward island) were necropsied. eight of these birds were in good body condition and died acutely from drowning or trauma. the remaining 23 birds were in poor body condition and had either chronic lead poisoning, respiratory mycosis, or oil contamination of their plumage. loons in poor body condition had significantly higher num ... | 1998 | 9706561 |
| concomitant mycotic and verminous pneumonia in a blue jay from georgia. | an emaciated, moribund, male blue jay (cyanocitta cristata) was collected in georgia (usa) and died shortly after capture. necropsy revealed nine large nematodes identified as diplotriaena tricuspis in the thoracic and abdominal air sacs and within the pericardial sac. multiple mycotic granulomas also were observed in the lungs and air sacs, and fungal culture of these lesions yielded pure growth of aspergillus fumigatus. histologic examination of the lungs revealed disseminated granulomatous in ... | 1998 | 9706574 |
| [qualitative and quantitative studies of autofluorescence in fungi]. | fluorescence microscopy is an important method in mycology. it is a common procedure used in immunology or histology and more recently in modern techniques of molecular biology like in-situ hybridization. since several molds and yeasts show autofluorescence, an interference of this phenomenon with the detection method cannot be excluded. therefore, we studied autofluorescence in fungi in more detail, in particular with respect to the dependence of this phenomenon from growth conditions, fixing m ... | 1998 | 9717385 |
| [molecular aspects and diagnostic value of fungal allergens]. | cloning, sequencing and production of highly pure recombinant allergens allows to produce perfectly standardised allergen preparations. the development of a new cloning system based on filamentous phage allowed the fast isolation and characterisation of allergens from the fungus aspergillus fumigatus. the produced recombinant allergens were tested in serological and clinical studies as well as for their performance for routine assessments in the immunocap-system. thereby, a perfect correlation b ... | 1998 | 9717388 |
| [the significance of secretory and structure-associated proteases of aspergillus fumigatus for the pathogenesis of invasive aspergillosis]. | in the course of invasive aspergillosis, aspergillus fumigatus is capable of penetrating any tissue of the host. secretory proteinases of the fungus might facilitate the hyphae to grow through fibrillar proteins like elastin and collagen. however, using systemic infection models, no significantly reduced virulence could be shown with fungal mutants deficient for all known secretory proteinases. thus, secretory proteinases might be of minor relevance for the pathogenesis of invasive aspergillosis ... | 1998 | 9717391 |
| skin-test reactivity and isotype-specific immune responses to recombinant asp f 3, a major allergen of aspergillus fumigatus. | the in vitro diagnostic value of recombinant allergens depends on the correlation between allergen specific ige in serum and ability of the protein to elicit immediate type allergic reactions in sensitized individuals. objective the study was carried out to evaluate the reliability of recombinant asp f 3 (rasp f 3)-based serological determinations in elisa and immunocap compared to skin-test responses in a. fumigatus sensitized subjects. | 1998 | 9720820 |
| cytokine- and t-helper-dependent immunity in murine aspergillosis. | 1998 | 9720962 | |
| pulmonary host defences against aspergillus fumigatus. | 1998 | 9720963 | |
| cytokines in allergic bronchopulmonary aspergillosis. | 1998 | 9720964 | |
| microbiological profile and potential hazards associated with imported and local brands of tomato paste in nigeria. | cans of three tomato paste brands (two of which are imported and one produced locally) showing defective or normal appearance were purchased from various retail outlets and analysed for microbial composition and ph values. substantially higher total viable counts were observed in samples from defective cans but the lowest population was found in the local brand. ratio of mesophilic to thermophilic micro-organisms increased in samples obtained from cans showing visible defects. anaerobic spore co ... | 1998 | 9721645 |
| disease-specific recombinant allergens for the diagnosis of allergic bronchopulmonary aspergillosis. | allergic bronchopulmonary aspergillosis (abpa), a severe pulmonary complication caused by aspergillus fumigatus, is considered a complex clinical syndrome with defined serological, pathological radiological and clinical features. the diagnosis of abpa is often difficult because of several overlapping clinical and laboratory findings shared between asthma with sensitization to a. fumigatus and abpa, but essential for treatment to prevent severe deterioration of pulmonary function. we have cloned ... | 1998 | 9723708 |
| acute community-acquired pneumonia due to aspergillus in presumably immunocompetent hosts: clues for recognition of a rare but fatal disease. | this article reports a case of acute community-acquired pneumonia due to aspergillus fumigatus in a healthy patient and reviews 11 previously reported cases occurring in presumably immunocompetent hosts. the diagnosis was delayed for all patients; mortality was 100%. clues that might suggest aspergillus as a pathogen in community-acquired pneumonia include a chest radiograph revealing diffuse infiltrates or new cavitation; lack of bacterial or viral cause; a preceding influenza a infection; and ... | 1998 | 9726758 |
| allergic bronchopulmonary aspergillosis. | allergic bronchopulmonary aspergillosis (abpa) is an immunologically mediated lung disease which occurs predominantly in patients with asthma, and is caused by hypersensitivity to colonized aspergillus fumigatus. it is a chronic, relapsing disorder which can clinically range from mild asthma to fibrotic lung disease. the immunopathogenesis of the disease is not clearly understood. early diagnosis and aggressive therapy with oral corticosteroids can prevent the development of fibrotic lung diseas ... | 1998 | 9727282 |
| characterization and optimization of in vitro assay conditions for (1,3)beta-glucan synthase activity from aspergillus fumigatus and candida albicans for enzyme inhibition screening. | (1,3)beta-d-glucan synthase (e.c.2.4.1.34. udp-glucose: 1,3-beta-d-glucan 3-beta-glucosyl transferase) catalyzes the polymerization of glucose ([1-3]-beta-linkages) using udp-glucose as substrate. we have determined optimal in vitro conditions for the assay of (1,3)beta-glucan synthase activity from aspergillus fumigatus and candida albicans. these included lysis of cells in the following for c. albicans, 100 mm hepes, ph 8.0, 10 microm guanosine 5'-o-(3-thiotriphosphate) (gtpgammas), 2 mm ethyl ... | 1998 | 9727393 |
| chest radiographic staging in allergic bronchopulmonary aspergillosis: relationship with immunological findings. | the question of whether a chest radiographic severity staging system could be correlated with standard blood/serum diagnostic indices in allergic bronchopulmonary aspergillosis (abpa) was addressed in 41 patients. asthma and positive aspergillus fumigatus (af) serology were considered essential diagnostic inclusion criteria. eosinophil count, serum immunoglobulin (ig)e and immediate skin hypersensitivity were also tested to grade patients as "definite" or "likely" abpa. definite cases had all fi ... | 1998 | 9727800 |
| primary cutaneous aspergillosis: our experience in 10 years. | we describe nine patients with primary cutaneous aspergillosis who were diagnosed during the 10-year period between 1987 and 1996. all except one were adults. seven of those nine cases had an immunocompromised state. among the immunocompromised patients, six had burn wounds and one was a renal transplant recipient. aspergillus flavus was isolated from seven patients and a. fumigatus from one, and in one other case the species could not be determined. three patients who had total burn surface are ... | 1998 | 9733373 |
| fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide d4e1. | the fungicidal properties of the synthetic peptide d4e1 were studied with nongerminated and germinating conidia of aspergillus flavus, aspergillus fumigatus, aspergillus niger, fusarium moniliforme, and fusarium oxysporum. the minimal lethal concentrations (mlc) needed to kill 100% of germinating conidia of a. fumigatus, a. flavus, and a. niger were 12.5, 12.5, and 25 microm, respectively. the mlc value for nongerminated and germinating conidia of both fusarium spp. was 3.0 microm. except for a. ... | 1998 | 9734302 |
| molecular probes for the detection of pathogenic fungi in the presence of human tissue. | four primer systems, amplifying fragments of the gene coding for the small ribosomal subunit (18s rrna) were characterised with pure cultures of 65 medically relevant fungal species plus two mushrooms. a primer cocktail (tr1/ca1-tr2/af2) amplified 59 of 67 fungal species; the universal fungal primer 1 (uf1) in combination with the eukaryotic primers s3 or eu1 amplified 64 and 65 of 67 fungal species, respectively. the design of an additional primer (rzy1) enabled the amplification of the missing ... | 1998 | 9736163 |
| activity of voriconazole combined with neutrophils or monocytes against aspergillus fumigatus: effects of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor. | voriconazole (vcz) was tested for antifungal activity against aspergillus fumigatus hyphae alone or in combination with neutrophils or monocytes. antifungal activity was measured as percent inhibition of hyphal growth in assays using the dye mtt [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] or xtt [2, 3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2h-tetrazolium-5-carboxa nilide ]. with both assays, vcz inhibited hyphal growth at concentrations of <1 microgram/ml and was almost as activ ... | 1998 | 9736553 |
| comparison of in vitro activities of the new triazole sch56592 and the echinocandins mk-0991 (l-743,872) and ly303366 against opportunistic filamentous and dimorphic fungi and yeasts. | the in vitro antifungal activities of sch56592, mk-0991, and ly303366 against 83 isolates of acremonium strictum, aspergillus flavus, aspergillus fumigatus, aspergillus terreus, bipolaris spp., blastomyces dermatitidis, cladophialophora bantiana, fusarium oxysporum, fusarium solani, histoplasma capsulatum, phialophora spp., pseudallescheria boydii, rhizopus arrhizus, scedosporium prolificans, and sporothrix schenckii were compared. the in vitro activities of these agents against 104 isolates of ... | 1998 | 9738049 |
| insertional mutagenesis of aspergillus fumigatus. | we have investigated transformation with heterologous dna as a method for insertional mutagenesis of aspergillus fumigatus. two methods, polyethylene glycol-mediated transformation of protoplasts and electroporation of germinating spores, were used to establish conditions leading to single-copy integration of transforming dna at different genomic sites. we have assessed the effect of restriction enzyme-mediated integration (remi) for both methods. non-remi protoplast transformation led to integr ... | 1998 | 9749676 |
| [chronic necrotizing pulmonary aspergillosis resistant to antimycotic drugs and treated by partial pulmonary resection]. | a 46-year-old man with a history of left upper lobectomy for pulmonary tuberculosis was admitted to our hospital because of dilated cardiomyopathy. during hospitalization, fever and weight loss developed. the cause was suspected to be a round mass inside a cavity and a neighboring infiltrative shadow in the left upper lung field as seen on chest radiography. a percutaneous needle biopsy was done, and examination of the specimen showed an aggregate of aspergillus fumigatus hyphae. fluconazole (fc ... | 1998 | 9754002 |
| granulocyte colony-stimulating factor and azole antifungal therapy in murine aspergillosis: role of immune suppression. | outbred icr mice were immune suppressed either with hydrocortisone or with 5-fluorouracil and were infected intranasally with aspergillus fumigatus. beginning 3 days before infection some groups of mice were given recombinant human granulocyte colony-stimulating factor (g-csf), sch56592 (an antifungal triazole), or both. corticosteroid-pretreated mice responded to sch56592 and had reduced counts in lung tissue and prolonged survival. in these mice, g-csf strongly antagonized the antifungal activ ... | 1998 | 9756743 |
| structure-in vitro activity relationships of pentamidine analogues and dication-substituted bis-benzimidazoles as new antifungal agents. | twenty analogues of pentamidine, 7 primary metabolites of pentamidine, and 30 dicationic substituted bis-benzimidazoles were screened for their inhibitory and fungicidal activities against candida albicans and cryptococcus neoformans. a majority of the compounds had mics at which 80% of the strains were inhibited (mic80s) comparable to those of amphotericin b and fluconazole. unlike fluconazole, many of these compounds were found to have potent fungicidal activity. the most potent compound again ... | 1998 | 9756747 |
| in vitro antifungal activities of a series of dication-substituted carbazoles, furans, and benzimidazoles. | aromatic dicationic compounds possess antimicrobial activity against a wide range of eucaryotic pathogens, and in the present study an examination of the structures-functions of a series of compounds against fungi was performed. sixty-seven dicationic molecules were screened for their inhibitory and fungicidal activities against candida albicans and cryptococcus neoformans. the mics of a large number of compounds were comparable to those of the standard antifungal drugs amphotericin b and flucon ... | 1998 | 9756748 |
| accumulation of amphotericin b in human macrophages enhances activity against aspergillus fumigatus conidia: quantification of conidial kill at the single-cell level. | a cytofluorometric assay that allowed assessment of damage to phagocytosed aspergillus fumigatus conidia at the single-cell level was developed. after ingestion by monocyte-derived macrophages (mdms), conidia were reisolated by treatment of the cells with streptolysin o, a pore-forming toxin with lytic properties on mammalian cells but not on fungi. the counts obtained by staining of damaged conidia with propidium iodide and quantification by cytofluorometry correlated with colony counts. by the ... | 1998 | 9756757 |
| [the role of imperfect fungi in etiopathogenesis of allergic rhinitis]. | in this paper the role of imperfect fungi in etiopathogenesis of perennial rhinitis was examined. in a group of 26 patients the concentration of total ige and ige specific of candida albicans, aspergillus fumigatus, alternaria alternata, mucor racemosus and cladosporum herbarum was signified. oversensibility to imperfect fungi was confirmed in 30.8% of patients: to candida albicans in 3.8%, to aspergillus fumigatus in 11.5%, to alternaria alternata in 3.8%, to mucor racemosus in 7.6% and to clad ... | 1998 | 9760768 |
| micellar electrokinetic chromatography: a convenient alternative to colorimetric and high performance liquid chromatographic detection to monitor protease activity. | high performance capillary electrophoresis (hpce) has been exploited as an analytical method alternative to current procedures for the determination of proteolytic activity of elastases from different sources. due to some drawbacks with capillary zone electrophoresis (cze), the mode of operation employed for the assay of elastolytic activity was micellar electrokinetic chromatography (mekc). using a background electrolyte consisting of 35 mm sodium tetraborate, ph 9.3, containing 65 mm sds and 1 ... | 1998 | 9761185 |
| effects of alkaline protease or restrictocin deficient mutants of aspergillus fumigatus on human polymorphonuclear leukocytes. | several substances including proteases and restrictocin have been suggested as candidates for virulence determinants in invasive pulmonary aspergillosis. however, the roles of such substances are not well understood. this study compared the in vitro suppressive effects of aspergillus fumigatus culture filtrates (acfs), on the functions of human polymorphonuclear leukocytes (pmnls), the principal cells in the host defence against aspergillus hyphae, from a clinically isolated wild-type and isogen ... | 1998 | 9762788 |
| probing the active site of mitogillin, a fungal ribotoxin. | fungal ribotoxins, such as mitogillin and the related aspergillus toxins restrictocin and alpha-sarcin, are highly specific ribonucleases, which inactivate the ribosome enzymatically by cleaving the eukaryotic 28s rna of the large ribosomal subunit at a single phosphodiester bond. the site of cleavage occurs between g4325 and a4326, which are present in a 14-base sequence (the alpha-sarcin loop) conserved among the large subunit rrnas of all living species. the amino acid residues involved in th ... | 1998 | 9767570 |
| skull base osteitis following fungal sinusitis. | aspergillus sp. sinusitis is not uncommon in immunocompromised patients but is unusual in patients who are not immunocompromised. the disease may occur as a saprophytic condition, as an allergic sinusitis or as a potentially lethal invasive disease. the differentiation between non-invasive and invasive aspergillus sp. sinusitis is crucial and this distinction is fully discussed. the treatment options are also considered. invasive disease requires aggressive treatment with long-term antifungal ag ... | 1998 | 9538458 |
| itraconazole for the treatment of pulmonary aspergillosis in heart transplant recipients. | the objective of this study was to evaluate the effects of itraconazole as a first choice drug in the treatment of pulmonary aspergillosis in heart transplant recipients. heart transplant recipients suffering from invasive pulmonary aspergillosis were included in this study. group 1 included 4 patients treated with i.v. itraconazole (janssen pharmaceutica) 400 mg daily, as a first choice drug for 28 d. itraconazole was discontinued and amphotericin-b was started before the 28th day if clinical o ... | 1998 | 9541420 |
| therapeutic efficacy of ashwagandha against experimental aspergillosis in mice. | therapeutic efficacy of an indian ayurvedic medicinal preparation, ashwagandha [withania somnifera l. dunal (solanceae; root)] was evaluated against experimental aspergillosis in balb/c mice. ashwagandha given orally once daily for 7 consecutive days in a dose of 100 mg/kg after intravenous infection of aspergillus fumigatus prolonged the survival period of infected mice. this protective activity was probably related to the observed increases in phagocytosis and intracellular killing of peritone ... | 1998 | 9543708 |
| cerebral aspergillosis in a liver transplant recipient: a case report of long-term survival after combined treatment with liposomal amphotericin b and surgery. | cerebral aspergillosis is a life-threatening complication in liver transplant recipients, with mortality rates approaching 100%; treatment with amphotericin b is of limited efficacy because of its poor distribution in the cerebrospinal fluid and its systemic side effects. we report the case of a liver transplant recipient who developed recurrent cerebral aspergillus fumigatus infection, and was successfully treated by combined surgical excision of the lesion and administration of liposomal ampho ... | 1998 | 9551693 |
| massive hemoptysis from a pulmonary artery aneurysm associated with an emphysematous bulla. | a 66-year-old man with a history of copd and aspergillus fumigatus infection developed massive hemoptysis. pulmonary artery angiography revealed an aneurysm which was successfully treated with coil embolization. this is the first known report of a pulmonary artery aneurysm causing massive hemoptysis in a patient with bullous emphysema. | 1998 | 9554660 |
| [nosocomial ventilator-associated pneumonias caused by aspergillus fumigatus in non-immunosuppressed, non-neutropenic patients]. | we observed three cases of severe ventilator-associated pneumonia due to aspergillus fumigatus in non-immunosuppressed, non-neutropenic patients. autopsy confirmed an angioinvasive pattern of spreading with septic foci. in all cases conditions associated with temporary immunodysfunction like underlying diseases, antecedent sepsis or pneumonia, coinfections or steroid therapy were present. although aspergillus fumigatus was isolated in all cases in cultures of tracheobronchial secretions, the cor ... | 1998 | 9557055 |
| efficacy of ly303366 against amphotericin b-susceptible and -resistant aspergillus fumigatus in a murine model of invasive aspergillosis. | ly303366 is a novel antifungal echinocandin with excellent in vitro activity against aspergillus spp. we compared four doses (1, 2.5, 10, and 25 mg/kg of body weight) of ly303366 with amphotericin b (0.5 to 5 mg/kg) in a temporarily neutropenic murine model of invasive aspergillosis against an amphotericin b-susceptible (af210) and an amphotericin b-resistant (af65) aspergillus fumigatus isolate based on in vivo response. mice were immunosuppressed with cyclophosphamide (200 mg/kg) and infected ... | 1998 | 9559799 |
| microgranulomatous aspergillosis in a patient with chronic granulomatous disease: cure with voriconazole. | 1998 | 9564492 | |
| differential ige recognition of recombinant aspergillus fumigatus allergens by cystic fibrosis patients with allergic bronchopulmonary aspergillosis or aspergillus allergy. | allergic bronchopulmonary aspergillosis (abpa), an intense inflammatory reaction to aspergillus in the lung, is recognized as a severe complication in patients with cystic fibrosis (cf). the diagnosis of abpa in cf patients sensitized to aspergillus fumigatus is complicated by interfering laboratory and clinical findings shared by the diseases. we have used cdna encoding a. fumigatus allergens which were cloned from a cdna library displayed on phage surface to produce recombinant proteins in esc ... | 1998 | 9565354 |
| the effectiveness of several current soft contact lens care systems against aspergillus. | fungal contamination of contact lenses is a common occurrence. in this study, we tested the effectiveness of currently used soft contact lens care systems against aspergillus fumigatus. | 1998 | 9571267 |
| effects of synthetic hydroxy isothiocyanates on microbial systems. | hydroxy isothiocyanates (itcs), including some new derivatives of naturally occurring compounds, were synthesized and their minimum inhibitory, minimum fungicidal, and minimum bactericidal concentrations for aspergillus niger, aspergillus fumigatus, staphylococcus aureus, and escherichia coli were estimated. these compounds were strongly antimicrobial; for example, 2-(4-hydroxyphenyl) ethyl itc inhibited growth of all strains examined at concentrations of 7.8 to 15.6 micrograms/ml. the atp conce ... | 1998 | 9571778 |
| molecular epidemiological study of aspergillus fumigatus in a bone marrow transplantation unit by pcr amplification of ribosomal intergenic spacer sequences. | we have developed a pcr-based method for the subspecific discrimination of aspergillus fumigatus types by using two primers designed to amplify the intergenic spacer regions between ribosomal dna transcription units. the method permitted the reproducible discrimination of 11 distinct dna types among a total of 119 isolates of a. fumigatus collected from patients and from the environment of a bone marrow transplantation (bmt) unit over a three-year period. ten dna types of a. fumigatus were isola ... | 1998 | 9574694 |
| polymerase chain reaction and restriction fragment length polymorphism mediated detection and speciation of candida spp causing intraocular infection. | to determine the usefulness of polymerase chain reaction (pcr) and restriction fragment length polymorphism (rflp) analysis in the identification and speciation of candida spp that causes ocular infection. | 1998 | 9579465 |
| extracellular phospholipases as universal virulence factor in pathogenic fungi. | microbial pathogens use a number of genetic strategies to invade the host and cause infection. these common themes are found throughout microbial virulence factors. secretion of enzymes, such as phospholipase, has been proposed as one of these themes which is used by bacteria, parasite, and pathogenic fungi. the role of extracellular phospholipase as a potential virulence factor in pathogenic fungi, including candida albicans, cryptococcus neoformans and aspergillus has gained credence recently. ... | 1998 | 9580028 |
| necrotizing external otitis caused by aspergillus fumigatus: computed tomography and high resolution magnetic resonance imaging in an aids patient. | most necrotizing (malignant) external otitis (neo) occurs in diabetic patients and is commonly caused by pseudomonas aeruginosa. we report an acquired immunodeficiency syndrome (aids) patient with neo caused by aspergillus fumigatus in which computed tomography (ct) showed destructive petrous bone involvement and magnetic resonance imaging (mri) of the ear discovered extensive soft tissue and facial nerve involvement. dedicated mri studies of the ear in this type of pathology provide new insight ... | 1998 | 9580133 |
| gastrointestinal ulceration and pulmonary aspergillosis in a llama treated for parelaphostrongylosis. | a 9-year-old llama examined because of hind limb paresis was found to have parelaphostrongylosis. despite treatment with ivermectin, fenbendazole, cimetidine, and ceftiofur, the llama developed gastrointestinal ulceration and pulmonary aspergillosis and was euthanatized. parelaphostrongylus tenuis is a parasite of white-tailed deer, but ruminants can serve as aberrant or dead-end hosts after accidentally ingesting snails or slugs carrying third-stage larvae of the parasite. gastrointestinal ulce ... | 1998 | 9589133 |
| lacrimal sac plugging caused by aspergillus fumigatus. | aspergillus fumigatus is a rare cause of canalicular obstruction. we present a case where an aspergillosis plug caused extreme tenderness, epiphoria and discharge from the lacrimal puntum. ct scan showed dilation of the lacrimal sac. medical treatment did not relieve the symptoms. during a planned dcr the sac was opened before the osteotomy, and found to contain a plug which caused the obstruction. aspergillus fumigatus was grown from culture. the dcr was abandoned and the patient is symptom fre ... | 1998 | 9591962 |
| mapping of the nitrate-assimilation gene cluster (crna-niia-niad) and characterization of the nitrite reductase gene (niia) in the opportunistic fungal pathogen aspergillus fumigatus. | this study reports the molecular characterization of the nitrate-assimilation gene cluster from the opportunistic fungal pathogen aspergillus fumigatus. a genomic fragment was isolated which contained the entire structural gene encoding nitrite reductase (niia), plus segments of the nitrate reductase (niad) and the nitrate transporter (crna) genes. nitrate-assimilation genes in a. fumigatus are physically linked and transcribed in the same direction as in a. nidulans. the nitrate-assimilation ge ... | 1998 | 9508795 |
| prostatic abscess due to aspergillus fumigatus: trus and mr imaging findings. | 1998 | 9514171 | |
| hospital construction-associated outbreak of ocular aspergillosis after cataract surgery. | this study aimed to report an outbreak of aspergillus endophthalmitis after cataract extraction during hospital construction. | 1998 | 9499785 |
| periantral soft-tissue infiltration and its relevance to the early detection of invasive fungal sinusitis: ct and mr findings. | our goal was to illustrate the relevance of periantral soft-tissue infiltration to the early diagnosis of invasive fungal sinusitis and to describe variations in the appearance of normal periantral soft tissues. | 1998 | 9504486 |
| concentration- and time-dependent upregulation and release of the cytokines mip-2, kc, tnf, and mip-1alpha in rat alveolar macrophages by fungal spores implicated in airway inflammation. | inhalation of fungal spores has been shown to cause primary or secondary infection and respiratory inflammation and diseases such as allergic alveolitis, atopic asthma, and organic dust toxic syndrome, which are rarely reported in the absence of predisposing factors. biochemical and molecular markers of inflammation were measured in rat bronchial alveolar lavage cells (> 95% macrophages) following stimulation with fungal spores isolated from pathogenic and nonpathogenic fungi that have been impl ... | 1998 | 9490662 |
| long-circulating immunoliposomal amphotericin b against invasive pulmonary aspergillosis in mice. | we investigated the efficacy of long-circulating immunoliposomal amphotericin b (amb) against invasive pulmonary aspergillosis in mice using three types of liposomal amb: conventional liposomal amb (ambisome), a long-circulating liposomal amb and prepared by coating the liposome surface with polyethylene glycol (peg; peg-l-amb), long-circulating immunoliposomal amb (34a-peg-l-amb). the survival rates for mice with invasive pulmonary aspergillosis treated with an intravenous dose of 2 mg of ambis ... | 1998 | 9449258 |
| acquired immunodeficiency syndrome with massive aspergillus fumigatus infection. | 1998 | 9482569 | |
| recombinant aspergillus fumigatus allergens: from the nucleotide sequences to clinical applications. | members of the genus aspergillus are opportunistic pathogens for cold- and warm-blooded animals. they are associated with an impressive spectrum of diseases in humans, ranging from benign colonization of the lung to life-threatening diseases such as invasive aspergillosis or allergic bronchopulmonary aspergillosis (abpa). aspergillus fumigatus is the etiological agent identified in most of the aspergillus-related human diseases and is therefore of particular clinical relevance. a major problem i ... | 1998 | 9482698 |
| allergic bronchopulmonary aspergillosis: disease pattern in central arabia. | despite a high prevalence of asthma in saudi arabia, allergic bronchopulmonary aspergillosis (abpa) has not been reported. we reviewed the medical records in a large university hospital in saudi arabia where thousands of asthmatics are being followed up. over a 9-year period starting january 1986, the diagnosis of abpa was made in 10 patients only. delay in diagnosis was common and in some patients the disease was confused with fungal pneumonia, tuberculosis or tumours. aspergillus fumigatus was ... | 1998 | 9484966 |
| aspergillus fungal mass detected by transesophageal echocardiography. | cardiac fungal infections have become more prevalent and are being diagnosed with increasing frequency. the most common infective organism is candida albicans, followed by aspergillus fumigatus and cryptococcus. cardiac involvement is usually associated with endocarditis, myocarditis, pericarditis, or intracardiac fungal mass. early diagnosis is imperative, as these patients have poor outcome once there is cardiac involvement. in this report we describe a patient in whom an intracardiac mass was ... | 1998 | 9487477 |
| human platelets damage aspergillus fumigatus hyphae and may supplement killing by neutrophils. | neutropenia is considered a significant risk factor for invasive aspergillosis but is almost always associated with concurrent thrombocytopenia. studies determined that platelets, like neutrophils, attached to cell walls of the invasive hyphal form of aspergillus fumigatus. organisms were damaged as shown by loss of cell wall integrity in scanning laser confocal microscopy and release of defined hyphal surface glycoproteins. rapid expression appearance of surface antigen cd63 and release of mark ... | 1998 | 9488412 |
| retained aspergillus-contaminated contact lens inducing conjunctival mass and keratoconjunctivitis in an immunocompetent patient. | a 66-year-old woman presented with a 1-month history of prominent mucoid discharge and foreign body sensation in her left eye. | 1998 | 9474456 |
| magnetic resonance image detection of coincidental sphenoid sinus aspergillosis and pituitary microadenoma: a potential surgical disaster. | while localizing the microadenoma in a patient with cushing's disease on magnetic resonance imaging (mri), fungal granuloma of the sphenoid sinus was coincidentally detected. this helped in the proper management of sphenoidal disease before undergoing trans-sphenoidal removal of the microadenoma, thus averting the possible catastrophe of direct intracranial spread of aspergillus infection. | 1998 | 9599827 |