Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| conserved surface features form the double-stranded rna binding site of non-structural protein 1 (ns1) from influenza a and b viruses. | influenza a viruses cause a highly contagious respiratory disease in humans and are responsible for periodic widespread epidemics with high mortality rates. the influenza a virus ns1 protein (ns1a) plays a key role in countering host antiviral defense and in virulence. the 73-residue n-terminal domain of ns1a (ns1a-(1-73)) forms a symmetric homodimer with a unique six-helical chain fold. it binds canonical a-form double-stranded rna (dsrna). mutational inactivation of this dsrna binding activity ... | 2007 | 17475623 |
| generation of the influenza b viruses with improved growth phenotype by substitution of specific amino acids of hemagglutinin. | variability in growth characteristics of influenza b viruses remains a serious limitation in the manufacture of inactivated influenza vaccines. currently, serial passage in eggs is the strategy used in most instances for selection of high growth virus variants. in previous studies we found that adaptation of the strain b/victoria/504/2000 to high growth in eggs was associated with changes only in hemagglutinin (ha). the high growth phenotype was associated with acquisition of either two (r162m a ... | 2007 | 17490701 |
| [two cases of successfully treated invasive pulmonary aspergillosis following influenza virus infection]. | while invasive pulmonary aspergillosis usually occurs in immunocompromised hosts, it has been described after influenza virus infection in healthy individuals. the first case was a 76-year-old previously healthy woman admitted because of chest pain, cough, sputum, fever, and a chest radiograph abnormality. a transbronchial biopsy specimen showed fungal hyphae. amphotericin b (amph) and itraconazole (itcz) were given, and she improved gradually. a viral test showed a titre of 1/128 to influenza a ... | 2007 | 17491315 |
| sensitive and specific detection of influenza virus a subtype h5 with real-time pcr. | avian influenza is a serious threat to both animal and human health. to enable cutting-edge research in this field, we developed a molecular test on the basis of real-time polymerase chain reaction (real-time pcr), which detects influenza virus rna. the test enables highly sensitive detection of influenza virus a and b strains, including h5n1, and specific identification of influenza a virus h5 subtypes. the kit was tested against a broad panel of influenza a and b subtypes and other respiratory ... | 2007 | 17494590 |
| carnitine, acylcarnitine and amino acid profiles analyzed by tandem mass spectrometry in a surfactant/virus mouse model of acute hepatic encephalopathy. | tandem mass spectrometry (ms/ms) was used to analyze multiple serum metabolites for the first time in a surfactant/virus mouse model of acute hepatic encephalopathy (ahe). ahe is characterized by acute liver failure that can lead to potentially lethal increases in intracranial pressure. we have reproduced ahe in young cd-1 mice exposed from postnatal day (p) 2-13 to the industrial surfactant, toximul 3409f (tox), and then infected intranasally on p14 with sublethal doses (ld(10-30)) of mouse-ada ... | 2007 | 17498775 |
| severe influenza resembling hemorrhagic shock and encephalopathy syndrome. | influenza-associated encephalopathy is a clinically diverse syndrome and severe cases are not well documented outside japan. clinical, pathological and molecular aspects are described of two fatal cases presenting during 2004 and 2005 winter seasons in the netherlands. results showed that severe influenza can resemble hemorrhagic shock and encephalopathy syndrome, and proper testing for influenza virus should be considered in similar cases. the failure to detect viral replication in non-pulmonar ... | 2007 | 17499546 |
| [rimantadine and arbidol sensitivity of influenza viruses that caused epidemic morbidity rise in russia in the 2004-2005 season]. | the study of the activity of arbidol against epidemic influenza a and b virus strains (2002-2005) in the cultured mdck cells showed the higher sensitivity of enzyme immunoassay than that of hemagglutination test. the influenza a virus strains tested, including those resistant to rimantadine (5 microg/ml), were sensitive to arbidol (10 microg/ml). the population of influenza b virus strains was heterogeneous in this indicator, 43% of the strains being less sensitive to arbidol. there was an incre ... | 2007 | 17500235 |
| influenza surveillance in victoria, 2006. | the victorian influenza season in 2006 remained within normal seasonal activity thresholds and was relatively mild compared with recent years. the season peaked in mid-august, with influenza-like illness (ili) rates from general practitioner sentinel surveillance and the melbourne medical locum service (mmls), and cases of laboratory-confirmed influenza notified to the department of human services, reaching their zeniths within one week of each other. a total of 74 general practitioners (gps) pa ... | 2007 | 17503649 |
| performance of directigen flu a+b enzyme immunoassay and direct fluorescent assay for detection of influenza infection during the 2004-2005 season. | early diagnosis of influenza infection is needed to optimize the benefit of prescribing antiviral drugs. however, the accuracy of rapid tests is highly variable. this study evaluated the performance of directigen flu a+b enzyme immunoassay (eia) and direct fluorescent assay (dfa) during the 2004-2005 influenza season. participants with medically attended acute respiratory illness were identified through an active surveillance. consenting patients (n=818) were enrolled and cultured for influenza. ... | 2007 | 17509800 |
| outcome of influenza vaccination in combat-related post-traumatic stress disorder (ptsd) patients. | post-traumatic stress disorder (ptsd) is an anxiety disorder that can occur after exposure to extreme traumatic experience such as war trauma, and is accompanied by fear, helplessness or horror. exposure to trauma can result in immune dysregulation and influence susceptibility to infectious disease as well as vaccine efficacy. the aim of the study was to determine the relation of psychological stress and the immune response to influenza vaccination in combat-related ptsd patients (n = 28). detec ... | 2007 | 17511777 |
| response of influenza vaccines against heterovariant influenza virus strains in adults with chronic diseases. | the ability of influenza vaccination to provide cross-protection against heterovariant influenza strains was evaluated in a double-blind, randomized, trial in north-east italy during the winter of 2005-2006. of 238 adult subjects with underlying chronic diseases, 120 received mf59-adjuvanted subunit vaccine (sub/mf59) and 118 received a conventional subunit vaccine (subunit). immunogenicity was measured for a/h3n2 and b influenza strains against both the homologous vaccine strains (a/new york/55 ... | 2007 | 17514499 |
| epidemics of influenza from winter to summer in the 2005/06 season in sapporo, japan. | 2007 | 17515659 | |
| zanamivir treatment is equally effective for both influenza a and influenza b. | 2007 | 17516418 | |
| flan: a web server for influenza virus genome annotation. | flan (short for flu annotation), the ncbi web server for genome annotation of influenza virus (http://www.ncbi.nlm.nih.gov/genomes/flu/database/annotation.cgi) is a tool for user-provided influenza a virus or influenza b virus sequences. it can validate and predict protein sequences encoded by an input flu sequence. the input sequence is blasted against a database containing influenza sequences to determine the virus type (a or b), segment (1 through 8) and subtype for the hemagglutinin and neur ... | 2007 | 17545199 |
| are point-of-care (poc) virological tests what is needed? | point-of-care (poc) tests are becoming more available, although the way in which they should be used is currently undecided. any 'laboratory'-based diagnosis of respiratory infections has three components: the specimen taken, the test used, and the interpretation of the results. each of these components needs to be carefully addressed when using poc tests for the diagnosis of respiratory tract infections. given the enthusiasm with which poc tests are being developed, it is likely that they will ... | 2007 | 17552976 |
| trends for influenza-related deaths during pandemic and epidemic seasons, italy, 1969-2001. | age-specific patterns of death from influenza vary, depending on whether the influenza season is epidemic or pandemic. we assessed age patterns and geographic trends in monthly influenza-related deaths in italy from 1969 through 2001, focusing on differences between epidemic and pandemic seasons. we evaluated age-standardized excess deaths from pneumonia and influenza and from all causes, using a modified version of a cyclical serfling model. excess deaths were highest for elderly persons in all ... | 2007 | 17553246 |
| clinical impact of influenza immunization in patients with liver cirrhosis. | influenza vaccine is considered to reduce influenza-related morbidity and mortality in patients with underlying chronic medical conditions. yet in liver cirrhosis, influenza vaccines have received little attention in determining the potential benefits. | 2007 | 17560166 |
| [diagnosis and epidemiological surveillance of influenza and respiratory syncytial virus infections: interest of multiplex pcr]. | respiratory infections require a rapid etiological diagnosis for efficient management of cases. we evaluated multiplex pcr used for the diagnosis and the epidemiological surveillance of influenza and respiratory syncytial virus (rsv) infections. | 2007 | 17560747 |
| proximal splenic artery embolization for blunt splenic injury: clinical, immunologic, and ultrasound-doppler follow-up. | to evaluate the clinical, us (ultrasound)-doppler and hematologic findings after proximal splenic artery embolization (psae) for blunt injury. | 2007 | 17563670 |
| clinical evaluation of the sd bioline influenza virus antigen test for rapid detection of influenza viruses a and b in children and adults during the influenza season. | the performance of the sd bioline rapid antigen test kit for influenza virus detection was evaluated with 295 respiratory specimens during the influenza season. the overall sensitivity and specificity of the sd bioline test were 61.9% and 96.8% for the influenza a virus antigen and 54.5% and 100% for the influenza b virus antigen, respectively. the results were consistent with peak influenza activities. | 2007 | 17567765 |
| john f. enders lecture 2006: antivirals for influenza. | the long history of influenza drug development has both contributed practical advances in antiviral chemotherapy and improved the understanding of influenza pathogenesis and epidemiology. the role played by these antivirals continues to grow with the dual threats of seasonal and pandemic influenza. the neuraminidase inhibitors are proven effective for the chemoprophylaxis and treatment of influenza a and b, although early therapy is essential for disease mitigation. studies of topically applied ... | 2007 | 17570104 |
| viral etiology of acute exacerbations of copd in hong kong. | viral respiratory infections may precipitate acute exacerbations of copd (aecopd). however, little is known about viral etiology related to aecopd in asia. we aimed to study the viral etiology of aecopd in hong kong. | 2007 | 17573516 |
| safety and tolerability of oseltamivir prophylaxis in hematopoietic stem cell transplant recipients: a retrospective case-control study. | oseltamivir is safe and effective in immunocompetent persons, and prophylactic use is recommended during influenza outbreaks. however, no data exist regarding the use of oseltamivir as prophylaxis among patients undergoing hematopoietic stem cell transplantation (hsct). | 2007 | 17578777 |
| influenzavirus infection is a primary cause of febrile respiratory illness in hiv-infected adults, despite vaccination. | there are, to our knowledge, no prospective studies of respiratory tract infections in human immunodeficiency virus (hiv)-infected adults in the highly active antiretroviral therapy (haart) era. we performed a surveillance study of outpatients who presented with fever and respiratory symptoms to examine the role of viral pathogens in these patients. | 2007 | 17578785 |
| influenza surveillance in new zealand in 2005. | we report the influenza activity in new zealand in 2005 (including an influenza b epidemic) in terms of the disease burden, hospitalisations, viral strain characterisations, and vaccine recommendations. | 2007 | 17589549 |
| blunted humoral response to influenza vaccination in patients exposed to zidovudine plus trimethoprim-sulfamethoxazole. | to determine as proof of principle the effect of combination exposure to zidovudine plus trimethoprim-sulfamethoxazole (tmp-smx) on humoral immune responses to influenza vaccination in patients with human immunodeficiency virus (hiv). | 2007 | 17594199 |
| [pcr restriction analysis of the genome composition of reassortant cold-adapted influenza b virus strains]. | a simple and sensitive method has been developed to determine the genome composition of the reassortant on the basis of b/ussr/60/69 by the restrictase analysis of dna copies of rna sites containing the nucleotide replacements typical of b/ussr/60/69. | 2007 | 17601045 |
| longer virus shedding in influenza b than in influenza a among outpatients treated with oseltamivir. | to compare the persistence, susceptibility and resistance of influenza a and influenza b viruses in oseltamivir therapy in outpatients of various ages. | 2007 | 17604839 |
| influenza and respiratory syncytial virus in infants and children: relationship with attendance at a paediatric emergency unit and characteristics of the circulating strains. | a study was carried out on 2,696 italian children, aged 0-14 years. the goals were: (1) to define the age-related impact of acute respiratory infections (ari), measured as the risk of attendance at the paediatric emergency room, (2) to better define the importance and proportion of influenza and respiratory syncytial virus (rsv) infections and (3) to acquire deeper knowledge of the influenza strains circulating in infants and children. a standardised emergency unit attendance risk (euar) was cal ... | 2007 | 17610094 |
| abnormal humoral immune response to influenza vaccination in pediatric type-1 human immunodeficiency virus infected patients receiving highly active antiretroviral therapy. | given that highly active antiretroviral therapy (haart) has been demonstrated useful to restore immune competence in type-1 human immunodeficiency virus (hiv-1)-infected subjects, we evaluated the specific antibody response to influenza vaccine in a cohort of hiv-1-infected children on haart so as to analyze the quality of this immune response in patients under antiretroviral therapy. sixteen hiv-1-infected children and 10 hiv-1 seronegative controls were immunized with a commercially available ... | 2007 | 17612772 |
| [efficacy of production of reassortant of epidemic strains and cold-adapted influenza viruses in chicken embryo and mdck cells]. | reassortant strains for modern live influenza vaccines are prepared using growing chicken embryos. it is very important to switch manufacture of influenza vaccines from chicken embryos to cell cultures, especially due to the threat of future pandemic, when there will be need of big quantities of vaccine for immunization of all age groups. efficacy of production of reassortant strains with 6:2 vaccine formulation of genome (6 internal genes from the donor of attenuation and 2 genes coding externa ... | 2007 | 18283733 |
| influenza a (h1n1) virus resistance to cyanovirin-n arises naturally during adaptation to mice and by passage in cell culture in the presence of the inhibitor. | influenza a/new caledonia/20/99 (h1n1) virus was studied for development of resistance to cyanovirin-n (cvn). cvn neutralizes virus infectivity by binding to specific high-mannose oligosaccharides on the viral haemagglutinin 1 (ha1) subunit. during virus adaptation to mice in the absence of cvn treatment the virus became resistant to cvn (cvn-mr virus), as did virus passaged in cell culture in the presence of cvn (cvn-r virus). the cvn-r virus possessed a single amino acid change at position 94a ... | 2007 | 18320936 |
| real-time rt-pcr assays for type and subtype detection of influenza a and b viruses. | influenza viruses type a (h3n2 and h1n1 subtypes) and b are the most prevalently circulating human influenza viruses. however, an increase in several confirmed cases of high pathogenic h5n1 in humans has raised concerns of a potential pandemic underscoring the need for rapid, point of contact detection. in this report, we describe development and evaluation of 'type,' i.e., influenza virus a and b, and 'subtype,' i.e., h1, h3, and h5, specific, single-step/reaction vessel format, real-time rt-pc ... | 2007 | 19432632 |
| antivirals for the treatment and prevention of epidemic and pandemic influenza. | influenza is a highly contagious and debilitating disease that imposes an excess burden of complications and mortality. antiviral therapy is the primary intervention for treatment and post-exposure prophylaxis (pep) of influenza. amantadine and rimantadine are members of the m2 class of antiviral agents and are moderately effective in influenza management. however, their utility is compromised by high levels of resistance, tolerability concerns and a lack of efficacy against influenza b. an alte ... | 2007 | 19453477 |
| influenza infection and risk of acute pulmonary embolism. | influenza infections have been associated with procoagulant changes. whether influenza infections lead to an increased risk of pulmonary embolism remains to be established. | 2007 | 17939867 |
| development and validation of a rapid hplc method for the determination of oseltamivir phosphate in tamiflu and generic versions. | oseltamivir phosphate (op) is an antiviral drug that is used in the treatment and prophylaxis of both influenza a and influenza b. it is effective against all known influenza viruses than can infect humans, including pandemic influenza viruses and may be the most appropriate antiviral option against avian influenza caused by h5n1 virus. tamiflu, the registered trademark used under exclusive license by roche laboratories with op as active pharmaceutical ingredient, is considered the best treatmen ... | 2007 | 17512688 |
| influenza b viruses isolated in uruguay during the 2002-2005 seasons: genetic relations and vaccine strain match. | monitoring antigenic and genetic variations of circulating influenza viruses is critical for the selection of annual vaccine strains. in order to gain insight into the molecular evolution of influenza b viruses (ibv) isolated in uruguay in 2002 and 2005 outbreaks, antigenic and phylogenetic studies were carried out for the hemagglutinin (ha) gene. antigenic relations among uruguayan and reference strains isolated elsewhere were performed by means of hemagglutination inhibition assays (hai). gene ... | 2007 | 16987563 |
| eccentric exercise as an adjuvant to influenza vaccination in humans. | the immune response to vaccination in animals can be enhanced by exposure to acute stress at the time of vaccination. the efficacy of this adjuvant strategy for vaccination in humans requires investigation. the current study employed a randomised controlled trial design to examine the effects of eccentric exercise prior to influenza vaccination on the antibody and cell-mediated responses. sixty young healthy adults (29 men, 31 women) performed eccentric contractions of the deltoid and biceps bra ... | 2007 | 16824730 |
| field performance and new uses of rapid influenza testing in thailand. | rapid influenza tests are increasingly used in surveillance systems and for clinical care in southeast asia. however, the performance and utility of rapid influenza tests under field conditions in rural southeast asia has not been evaluated. | 2007 | 16798041 |
| medically attended pediatric influenza during the resurgence of the victoria lineage of influenza b virus. | during the 2002-2003 season, a new variant of influenza b co-circulated with influenza a viruses. this study examines the characteristics and outcomes of children with influenza a and b virus infection vs. other acute respiratory illnesses. | 2007 | 16678464 |
| semiquantitative one-step rt-pcr for simultaneous identification of human influenza and respiratory syncytial viruses. | a multiplex reverse transcription-polymerase chain reaction (mrt-pcr) method was developed for simultaneous detection and typing/subtyping of influenza viruses a/h1, a/h3 or b, and respiratory syncytial viruses a or b, followed by dna semiquantitation using the agilent 2100 bioanalyzer. such method provides a rapid, specific and sensitive diagnostic tool for detection and semiquantification of respiratory illness specimens. | 2007 | 17030067 |
| synthesis and biological evaluation of dihydrofuran-fused perhydrophenanthrenes as a new anti-influenza agent having novel structural characteristic. | dihydrofuran-fused perhydrophenanthrenes were synthesized by means of o-quinodimethane chemistry with high generality and stereoselectivity, and found to exhibit potent anti-influenza activity. these compounds exerted an inhibitory effect on various strains of influenza virus growth, including influenza a and b, with a concentration dependent manner, and direct cytotoxicity was low. several biological experiments suggested that these new drugs affected a virus replication process before mrna syn ... | 2007 | 17035034 |
| antibody responses after dose-sparing intradermal influenza vaccination. | reduced-dose intradermal (id) influenza vaccination is an attractive approach to increase availability of vaccine supply in an event of vaccine shortage. we conducted a randomized open-label study, in which 500 subjects were randomly assigned to receive an id injection of 0.1 ml dose of inactivated split-virion influenza vaccine or an im injection of 0.5 ml dose. the subjects who had hemagglutination inhibition (hi) antibody titer of at least 1:40 at day 28 post-vaccination in id and im groups w ... | 2007 | 17011678 |
| exploration of the emergence of the victoria lineage of influenza b virus. | the victoria lineage represented by b/victoria/2/87 is one of the two major distinctive haemagglutinin (ha) lineages of influenza b virus, and its recent re-emergence has aroused great concerns. however, it remains unknown when, where, and how this ha lineage emerged in the world. in this study, the ha1 domain of the ha gene of fourteen influenza b viruses isolated in china in 1972-1984 was sequenced. the sequences were phylogenetically analyzed with the ha1 sequences of 41 other important influ ... | 2007 | 17075722 |
| estimating vaccine effectiveness against laboratory-confirmed influenza using a sentinel physician network: results from the 2005-2006 season of dual a and b vaccine mismatch in canada. | we report a case-control design using a sentinel physician network to estimate vaccine effectiveness (ve) against laboratory-confirmed, medically attended influenza (lc-mai) and provide results for the 2005-2006 season of dual a and b vaccine mismatch in canada. | 2007 | 17081662 |
| anti-influenza virus activity of biflavonoids. | ginkgetin was found to inhibit the influenza virus sialidase. ginkgetin-sialic acid conjugates showed a significant survival effect in the influenza-virus-infected mice. | 2007 | 17110111 |
| appropriate measures of influenza immunization program effectiveness. | groll and thomson's evaluation of the effectiveness of ontario's universal influenza immunization campaign used per capita cases of laboratory-confirmed influenza. we argue that these data are susceptible to various biases and should not be used as an outcome measure. laboratory data are traditionally used to identify the presence of influenza activity rather than to identify levels of influenza activity. a better measure of viral activity is the proportion of influenza tests positive; whereas t ... | 2007 | 17052813 |
| [influenza season 2007/'08 in the netherlands: antigenic variation, oseltamivir resistance and vaccine composition for the 2008/'09 season]. | the first signs of influenza activity during the 2007/'08 influenza season in the netherlands were sporadic isolations of influenza viruses between week 40 and week 52 of 2007. the frequency of virus isolations and clinical influenza activity increased after week 1 of 2008 and peaked around week 9. in this week, 7.2 patients with influenza-like illness were recorded per 10,000 inhabitants. the influenza epidemic was caused primarily by influenza a/h1n1 viruses and influenza b viruses. two antige ... | 2008 | 18856032 |
| [surveillance for influenza b virus infections in infants and young children in beijing, china]. | to characterize the prevalence of influenza b virus infection in infants and young children in beijing. | 2008 | 19099679 |
| [application of fluorescent real-time polymerase chain reaction in analyzing the epidemic of influenza among children in guangzhou area in 2006]. | to investigate the prevalence of influenza virus infections in children in 2006 using the real-time pcr method. | 2008 | 19099836 |
| a vaccine manufacturer's approach to address medical needs related to seasonal and pandemic influenza viruses. | vaccination is considered to be one of the most effective tools to decrease morbidity as well as mortality caused by influenza viruses. for the prevention of seasonal influenza, fluarix and flulaval have been marketed since 1987 and 1992, respectively. both vaccines have consistently been shown to meet or exceed the regulatory criteria for immunogenicity against the three strains h1n1, h3n2 and b, have a good safety profile, and are recommended for vaccinating children and adults of all ages. fo ... | 2008 | 19453402 |
| epidemiological study of influenza virus infections in young adult outpatients from buenos aires, argentina. | influenza virus is the most common cause of influenza-like illness (ili) in adults. in argentina, studies on influenza and other respiratory viruses were performed mostly in pediatric populations. | 2008 | 19453464 |
| immunogenicity of a reduced dose of a/h3n2 in the 2005 southern hemisphere formulation of inactivated split influenza vaccine. | the 2005 southern hemisphere formulation of the inactivated split-virion influenza vaccine vaxigrip unintentionally contained a lower concentration of haemagglutinin (ha) than european pharmacopoeia (ep) and who specifications for one of the three strains. | 2008 | 19453468 |
| children hospitalized with influenza during the 2006-2007 season: a report from the canadian immunization monitoring program, active (impact). | 2008 | 19368059 | |
| comparative analysis of reproduction of influenza virus strains in cell lines perspective for the creation of cultural vaccines grown on nutrient medium on the basis of rise flour protein hydrolysate. | we studied vaccine strains of influenza viruses a and b during their culturing in mdck and vero cells grown in eagle's mem medium and in a medium on the basis of enzyme hydrolysate of rise flour proteins with reduced (2%) content of fetal calf serum. optimal conditions for cell culturing and reproduction of influenza virus strains in these cells were studied. culturing of vaccine strains of influenza viruses in mdck and vero cells grown in nutrient media on the basis of rise flour protein hydrol ... | 2008 | 19489339 |
| evaluation of the nanochip 400 system for detection of influenza a and b, respiratory syncytial, and parainfluenza viruses. | the nanochip400 system uses multiplex pcr chemistry and electronic microarray detection of influenza a and b viruses; respiratory syncytial viruses a and b; and human parainfluenza virus types 1, 2, and 3. the results obtained with the nanochip 400 system were compared with those obtained by direct fluorescent-antibody staining (dfa) and real-time pcr with 122 and 130 specimens, respectively. concordance between dfa and nanochip 400 system was obtained for 106 of 122 (86.9%) specimens. on the ba ... | 2008 | 18322066 |
| comparisons of the influenza virus a m2 channel binding affinities, anti-influenza virus potencies and nmda antagonistic activities of 2-alkyl-2-aminoadamantanes and analogues. | the new 2-alkyl-2-aminoadamantanes and analogues 4-10 were designed and synthesized by simplification of the structure of the potent anti-influenza virus a spiranic aminoadamantane heterocycles 2 and 3. the aim of the present work was to examine the effects of bulky and extended lipophilic moieties attached to amantadine 1 on binding to the m2 channel and the resulting antiviral potency. the binding affinities of the compounds to the m2 protein of influenza virus a/chicken/germany/27 (weybridge ... | 2008 | 18947998 |
| the potential impact of neuraminidase inhibitor resistant influenza. | neuraminidase inhibitor resistant influenza virus has recently emerged, and circulated, in untreated persons. influenza virus evolution is causing antiviral susceptibility to change. we review the latest research in this rapidly moving field. | 2008 | 18978531 |
| the real spin on global flu. | 2008 | 18988552 | |
| anti-influenza virus activity of myrica rubra leaf ethanol extract evaluated using madino-darby canine kidney (mdck) cells. | myrica rubra leaf ethanol extract was added to culture medium of madino-darby canine kidney (mdck) cells inoculated with influenza virus, and the inhibition of influenza virus replication was measured. myrica rubra leaf ethanol extract showed anti-influenza virus activity irrespective of the hemagglutinin antigen type in the influenza virus type a (h1n1), its subtype (h3n2), and type b. | 2008 | 18997411 |
| fatal necrotizing pneumonia due to a panton-valentine leukocidin positive community-associated methicillin-sensitive staphylococcus aureus and influenza co-infection: a case report. | recent studies have described a number of fatalities due to methicillin-resistant staphylococcus aureus (mrsa) and influenza virus co-infection. mrsa isolates provide a challenge to caregivers due to inherent wide range antibiotic resistance. many facilities have instituted screening methods, based on the presence of antibiotic resistance genes, to identify mrsa positive patients upon admission. however, the resistance profile of the pathogen does not necessarily determine the severity of diseas ... | 2008 | 18284686 |
| effectiveness of oseltamivir treatment among children with influenza a or b virus infections during four successive winters in niigata city, japan. | oseltamivir has been used for treatment of influenza a and b infections, but recent reports documented that it was less active against the latter. we compared the effectiveness of oseltamivir in children between laboratory confirmed influenza a and b over 4 influenza seasons from 2001 to 2005 in a pediatric clinic in japan. among 1,848 patients screened, 299 influenza a and 209 influenza b patients were administered oseltamivir (treated groups), and 28 influenza a and 66 influenza b patients wer ... | 2008 | 18285668 |
| update: influenza activity--united states, september 30, 2007-february 9, 2008. | this report summarizes u.s. influenza activity since the beginning of the 2007-08 influenza season (september 30, 2007) and updates the previous summary. from september through early december, influenza activity remained low in the united states. activity increased from early december through the end of the year and has continued to increase in january and february. | 2008 | 18288077 |
| rapid diagnosis of influenza infection in older adults: influence on clinical care in a routine clinical setting. | laboratory diagnosis of influenza has previously relied on viral isolation in culture. rapid antigen tests (rats) are now available but few studies have examined their use in older adults under routine clinical conditions. | 2008 | 18289930 |
| on the mechanism of phenotypic conversion of human cervical adenocarcinoma hela cells surviving infection by influenza b virus: potential implications for biological management of adenocarcinomas. | we infected hela cells with low (10(-9) units), medium (10(-6) units), and high (10(-2) units) influenza b titers and compared the resulting human papilloma virus (hpv), retinoic acid receptor alpha subunit (raralpha) and glyceraldehyde-3-phosphate dehydrogenase (gapdh) mrna content of surviving infected hosts with that of their uninfected precursors by semi-quantitative reverse transcriptase/polymerase chain reaction amplification (rt/pcr). this comparison revealed a moderate and drastic depend ... | 2008 | 18291599 |
| recommended composition of influenza virus vaccines for use in the 2008-2009 influenza season. | 2008 | 18309579 | |
| [cytokine profile and level of antibodies after administration of split-vaccine against influenza to adults]. | serum concentrations of cytokines (ifn-gamma, tnf-alpha, il-2, il-4, il-6, il-8) and antibody levels to influenza virus antigens were studied in adults vaccinated with split-vaccine against influenza. analysis of antibody titers 21 days after vaccination compared with baseline levels revealed high immunogenicity of vaccine in terms of mean geometric titers increase as well as sufficient levels of seroprotection and seroconversion. study of cytokine profile showed absence of significant changes o ... | 2008 | 19006832 |
| universal antibodies and their applications to the quantitative determination of virtually all subtypes of the influenza a viral hemagglutinins. | the fusion peptide is the only universally conserved sequence in the hemagglutinins of all 16 subtypes of influenza a and two genetic lineages of influenza b viruses. here, peptides selected by bioinformatics approach were modified and conjugated to overcome serious technical hurdles such as the high hydrophobicity and weak immunogenicity of the viral fusion peptides. antibodies generated against fusion peptides demonstrated remarkable specificity against the viral sequences and robustness of qu ... | 2008 | 19007587 |
| [resistance in influenza viruses]. | influenza virus infection can be prevented and treated with antiviral drugs. the usage of such drugs in norway has been infrequent, however, they are an important component in our pandemic preparedness planning, as it will probably be difficult to get access to the appropriate vaccine in time before the pandemic reaches the country. the first generation of influenza drugs acquired resistance to a large degree, in contrast to the modern neuraminidase inhibitors that until recently have had minor ... | 2008 | 19023375 |
| update: influenza activity--united states, september 28-november 29, 2008. | during september 28-november 29, 2008, influenza activity remained low in the united states. of the few influenza viruses characterized thus far this season, most are antigenically related to the strains included in the 2008-09 influenza vaccine. oseltamivir-resistant influenza a (h1n1) viruses have been detected, but currently available data are insufficient to predict their prevalence for the 2008-09 season. this report summarizes u.s. influenza activity since the last update and reviews new i ... | 2008 | 19078922 |
| mechanism of nkt cell activation by intranasal coadministration of alpha-galactosylceramide, which can induce cross-protection against influenza viruses. | in a nasal vaccine against influenza, the activation of natural killer t (nkt) cells by intranasal coadministration of alpha-galactosylceramide (alpha-galcer) can potently enhance protective immune responses. the results of this study show that the nkt cell-activated nasal vaccine can induce an effective cross-protection against different strains of influenza virus, including h5 type. to analyze the mechanism of nkt cell activation by this nasal vaccine, we prepared fluorescence-labeled alpha-ga ... | 2008 | 19079180 |
| rate of influenza vaccination and its adverse reactions seen in health care personnel in a single tertiary hospital in korea. | to determine the vaccination rate and its adverse reactions after influenza vaccination, we administered an anonymous questionnaire survey during the last three influenza seasons from 2005-2006 to 2007-2008. in total, the rate of influenza vaccination was 82.3% in health-care personnel. dividing the subjects into four groups by work category, the vaccine coverage rates were as follows: physicians 67.9%; nurses and nursing assistants 91.2%; technicians, pharmacists, therapists, and administrative ... | 2008 | 19050354 |
| faster, more sensitive flu tests to combat flu pandemic. | 2008 | 19054896 | |
| [the spread and biological properties of epidemic influenza viruses a and b strains circulating in the 2006-2007 season in russia]. | the epidemic upsurge of influenza morbidity in the 2006-2007 season in russia was caused by the active circulation of influenza a(h1n1), a(h3n2) and b viruses. the center for ecology and epidemiology of influenza studied 259 epidemic strains; all the strains were isolated on mdck cell cultures. influenza a(h1n1) viruses (n = 101) were antigenic variants of the references a/new caledonia/20/99 and a/solomon islands/3/06. influenza a(h3n2) viruses (n = 98) were antigenic variants of the references ... | 2008 | 19069788 |
| [analysis of influenza surveillance from 2000 to 2007 in gansu province]. | to study the epidemical characteristics of influenza in gansu province from 2000 to 2007, and to discuss the changes of the predominant strains of influenza virus. this study provide scientific basis for defending influenza effectively. | 2008 | 19105339 |
| [primary research of anti-influenza virus activity of "benovoair concentrate"]. | anti-influenza virus activity of "benovoair concentrate". | 2008 | 19105340 |
| co-administration of l-cystine and l-theanine enhances efficacy of influenza vaccination in elderly persons: nutritional status-dependent immunogenicity. | the immune response to influenza vaccine is attenuated in elderly persons, though they are at greatest risk for morbidity and mortality by influenza virus infection. experimental studies demonstrate that co-administration of l-cystine and l-theanine enhanced antigen-specific production of immunoglobulin in aged mice infected with influenza virus. we thus investigated the effect of l-cystine and l-theanine on antibody induction by influenza vaccines in elderly persons. | 2008 | 19149835 |
| is there a role for influenza vaccination in cystic fibrosis? | influenza vaccination is generally recommended to patients with cystic fibrosis (cf). previous studies have shown that influenza infections cause worsening lung functions, disease progression and increase propensity of bacterial infections in cf. however, the clinical evidence in the effectiveness of influenza vaccination in cf is lacking. | 2008 | 17616444 |
| the effect of anti-tumour necrosis factor alpha treatment on the antibody response to influenza vaccination. | the effect of anti-tumour necrosis factor (tnf) therapy on the antibody responses to vaccines is the subject of ongoing debate. therefore, we investigated the effect of the three currently available anti-tnf agents on influenza vaccination outcomes in a patient population with long-standing disease. | 2008 | 17965123 |
| linking syndromic surveillance with virological self-sampling. | calls to a uk national telephone health helpline (nhs direct) have been used for syndromic surveillance, aiming to provide early warning of rises in community morbidity. we investigated whether self-sampling by nhs direct callers could provide viable samples for influenza culture. we recruited 294 nhs direct callers and sent them self-sampling kits. callers were asked to take a swab from each nostril and post them to the laboratory. forty-two per cent of the samples were returned, 16.2% were pos ... | 2008 | 17394678 |
| vaccination against influenza in patients with rheumatoid arthritis: the effect of rituximab on the humoral response. | to assess the effect of rituximab on the efficacy and safety of influenza virus vaccine in patients with rheumatoid arthritis (ra). | 2008 | 17981914 |
| cytoplasmic domain of influenza b virus bm2 protein plays critical roles in production of infectious virus. | influenza b virus bm2 is a type iii integral membrane protein that displays h(+) ion channel activity. analysis of bm2 knockout mutants has suggested that this protein is a necessary component for the capture of m1-viral ribonucleoprotein (vrnp) complex at the plasma membrane and for incorporation of vrnp complex into the virion during the assembly process. bm2 comprises 109 amino acid residues and possesses a longer cytoplasmic domain than the other 3 integral membrane proteins (hemagglutinin, ... | 2008 | 17989175 |
| the molecular epidemiology of influenza viruses: a lesson from a highly epidemic season. | to analyse the epidemiological and molecular features of a long-lasting epidemic (12 weeks) of influenza in north-eastern italy during the 2004-05 season. | 2008 | 17630401 |
| detection of human influenza a (h1n1) and b strains with reduced sensitivity to neuraminidase inhibitors. | neuraminidase inhibitors (nais) have been used since 2005 in france. | 2008 | 18055253 |
| evaluation of the one-step multiplex real-time reverse transcription-pcr proflu-1 assay for detection of influenza a and influenza b viruses and respiratory syncytial viruses in children. | we evaluated the one-step multiplex real-time reverse transcription-pcr proflu-1 assay for the detection of influenza a and influenza b viruses and respiratory syncytial viruses from 353 pediatric nasopharyngeal aspirates. as assessed by comparison with the results of immunofluorescence testing and cell culture, the specificity and the sensitivity of the proflu-1 assay ranged from 97% to 100%. in addition, the proflu-1 assay amplified 9% of samples not detected by conventional methods. | 2008 | 18057126 |
| performance of binax now flu a and b and direct fluorescent assay in comparison with a composite of viral culture or reverse transcription polymerase chain reaction for detection of influenza infection during the 2006 to 2007 season. | the binax now flu a and flu b (binax now), direct fluorescent assay (dfa), and viral culture were evaluated and compared with a composite of viral culture or reverse transcription polymerase chain reaction (rt-pcr). participants with medically attended acute respiratory illness were identified through active surveillance during the 2006 to 2007 season, and consenting individuals (n=932) were tested for influenza by culture and rt-pcr. physicians ordered a rapid antigen test (binax now [n=73] or ... | 2008 | 18060723 |
| dual infections by influenza a/h3n2 and b viruses and by influenza a/h3n2 and a/h1n1 viruses during winter 2007, corsica island, france. | the investigation of dual influenza infection human cases is of major interest specifically for the control of new emerging influenza strains. | 2008 | 18069055 |
| molecular epidemiology and disease severity of respiratory syncytial virus in relation to other potential pathogens in children hospitalized with acute respiratory infection in jordan. | human respiratory syncytial virus (hrsv) is the major viral cause of acute lower respiratory tract infections in children. few data about the molecular epidemiology of respiratory syncytial virus in developing countries, such as jordan, are available. the frequency and severity of infections caused by hrsv were assessed in hospitalized jordanian children <5 years of age compared with other potential etiological agents. overall a potential pathogen was detected in 78% (254/326) of the children. h ... | 2008 | 18041044 |
| a comparison of the effectiveness of zanamivir and oseltamivir for the treatment of influenza a and b. | to compare the effectiveness of zanamivir with oseltamivir for influenza a and b. | 2008 | 17936910 |
| distinct rig-i and mda5 signaling by rna viruses in innate immunity. | alpha/beta interferon immune defenses are essential for resistance to viruses and can be triggered through the actions of the cytoplasmic helicases retinoic acid-inducible gene i (rig-i) and melanoma differentiation-associated gene 5 (mda5). signaling by each is initiated by the recognition of viral products such as rna and occurs through downstream interaction with the ips-1 adaptor protein. we directly compared the innate immune signaling requirements of representative viruses of the flaviviri ... | 2008 | 17942531 |
| severe respiratory disease and its relationship with respiratory viruses in colombia. | there are important gaps in our understanding of the epidemiology of respiratory virus infections in tropical countries. in september 2003, the colombian epidemiological surveillance system was notified of several deaths from an acute respiratory disease (ard). | 2008 | 17720600 |
| ten years of viral and non-bacterial serology in adults with cystic fibrosis. | viral infections are associated with pulmonary exacerbations in children with cystic fibrosis (cf), but few studies have addressed the frequency in adults. this paper investigates the frequency and impact of viral infections in adults with cf receiving intravenous antibiotics. pre- and post-treatment spirometry, inflammatory markers and antibody titres against influenza a, influenza b, adenovirus, respiratory syncytial virus, mycoplasma pneumoniae, chlamydia psittaci, and coxiella burnetti were ... | 2008 | 17352838 |
| is exercise protective against influenza-associated mortality? | little is known about the effect of physical exercise on influenza-associated mortality. | 2008 | 18461130 |
| essential sequence of influenza b virus hemagglutinin dna to provide protection against lethal homologous viral infection. | hemagglutinin (ha) is the main surface glycoprotein of influenza b virus. the b/ibaraki/2/85 virus ha gene is 1758 bp in length, including signal peptide sequence, ha1 sequence, and ha2 sequence. we previously proved that b/ibaraki/2/85 ha dna induced immune response and provided effective protection in mice against challenge with homologous virus. in this study, a series of recombinant plasmids encoding truncated ha gene were constructed by pcr. balb/c mice were immunized with the plasmids and ... | 2008 | 18466089 |
| enhanced detection of respiratory viruses using cryopreserved r-mix readycells. | r-mix, which contains a fresh mixture of two cell lines, mv1lu (mink lung cells) and a549 cells, has shown good sensitivity and specificity for respiratory virus culture. however, it has until recently only been available in north america, in part due to the shipping constraints associated with cell aging and the difficulty in providing these cells to hard to reach regions. recently, cryopreserved r-mix readycells for longer storage were developed. these cells, which are shipped on dry ice and h ... | 2008 | 18467164 |
| mdck-siat1 cells show improved isolation rates for recent human influenza viruses compared to conventional mdck cells. | the ability to isolate and propagate influenza virus is an essential tool for the yearly surveillance of circulating virus strains and to ensure accurate clinical diagnosis for appropriate treatment. the suitability of mdck-siat1 cells, engineered to express increased levels of alpha-2,6-linked sialic acid receptors, as an alternative to conventional mdck cells for isolation of circulating influenza virus was assessed. a greater number of influenza a (h1n1 and h3n2) and b viruses from stored hum ... | 2008 | 18480230 |
| the evolutionary dynamics of human influenza b virus. | despite their close phylogenetic relationship, type a and b influenza viruses exhibit major epidemiological differences in humans, with the latter both less common and less often associated with severe disease. however, it is unclear what processes determine the evolutionary dynamics of influenza b virus, and how influenza viruses a and b interact at the evolutionary scale. to address these questions we inferred the phylogenetic history of human influenza b virus using complete genome sequences ... | 2008 | 18504518 |
| homologous recombination is unlikely to play a major role in influenza b virus evolution. | influenza b viruses cause a significant amount of morbidity and mortality. the occurrence of homologous recombination in influenza viruses is controversial. to determine the extent of homologous recombination in influenza b viruses, recombination analyses of 2,650 sequences representing all eight segments of the influenza b viruses were carried out. only four sequences were indentified as putative recombinants, which were verified using phylogenetic methods. however, the mosaics detected here we ... | 2008 | 18505573 |
| clinical features of children infected with different strains of influenza b in southern taiwan. | this study was designed to determine the clinical characteristics of children infected with different strains of influenza b viruses isolated in southern taiwan. the clinical features were compared with influenza a infection occurring in the same period. | 2008 | 18520968 |
| influenza viruses with reduced sensitivity to the neuraminidase inhibitor drugs in untreated young children. | the neuraminidase inhibitors are a class of antiviral drugs used for both the prophylaxis and treatment of influenza infections. clinical trials of these inhibitors detected a low level of resistant viruses from treated individuals, although a higher frequency was detected in children (5%-6%) compared to adults (1%-4%). in addition, there have been some previous reports of na inhibitor resistant viruses being isolated from untreated individuals. here we report on the na inhibitor sensitivity of ... | 2008 | 18522305 |