Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| parotitis and pancreatitis complicating infectious mononucleosis. | 1982 | 6286529 | |
| the laboratory approach to the diagnosis of infectious mononucleosis. | 1982 | 6286532 | |
| detection and characterization of ebv antigens by micro-elisa and chromatofocusing. | a micro-elisa technique was developed for the detection of epstein-barr virus (ebv)-determined antigens. the enzyme-linked immunosorbent assay (elisa) was applied with peroxidase-protein a to detect the antigens adsorbed to micro-elisa plates. human and rabbit antisera containing antibodies to known ebv components were used as reagents. the early antigen (ea) complex, associated with the viral cycle, was readily detected in extracts of n-butyrate- or n-butyrate + tpa-induced cells. the nuclear a ... | 1982 | 6286703 |
| immunoglobulin secretion by cell lines derived from african and american undifferentiated lymphomas of burkitt's and non-burkitt's type. | as part of an investigation of the cellular origins of undifferentiated lymphomas of the burkitt's and non-burkitt's types, we have examined immunoglobulin secretion by cell lines and biopsy samples from these tumors and have compared it with other lymphoblastoid cell lines. the majority of american lymphoma cell lines and a smaller fraction of african lymphoma cell lines secreted monoclonal igm. no other ig classes were secreted. ig secretion by the tumor samples was also found to be exclusivel ... | 1982 | 6286763 |
| molecular interactions in human t cell-mediated cytotoxicity to ebv ii. monoclonal antibody okt3 inhibits a post-killer-target recognition/adhesion step. | 1982 | 6286765 | |
| antigen presentation by human b cells: t cell proliferation induced by epstein barr virus b lymphoblastoid cells. | t cell proliferation in response to antigen requires the presence of an antigen-presenting accessory cell. the accessory cell most commonly associated with antigen presentation has been the macrophage (mo). this study demonstrates that b cells in the form of epstein barr virus-transformed b lymphoblasts (ebv-b) are able to present tetanus toxoid (tt) to human t cells and induce proliferation of these cells. t cells freshly isolated from peripheral blood were shown to undergo blast transformation ... | 1982 | 6286767 |
| generation of long-term human cytolytic cell lines with persistent natural killer activity. | human cell lines maintained by in vitro stimulation with the hla-a, b-negative, dr-positive, epstein barr virus-transformed, lymphoblastoid cell line daudi in the presence of conditioned medium demonstrated significant nk activity for over 6 wk in continuous culture. these cells lyse k562 and a broad panel of lymphoblastoid cell lines but do not lyse normal peripheral blood lymphocytes or pokeweed mitogen blasts. they possess the sheep red blood cell receptor but lack other t cell markers (lyt-3 ... | 1982 | 6286775 |
| the role of lymphocytes in infections due to epstein-barr virus and cytomegalovirus. | 1982 | 6286793 | |
| use of epstein-barr virus-transformed b cell lines for the generation of immunoglobulin-producing human b cell hybridomas. | hgprtase-deficient ebv-transformed b cell lines were shown to be effective fusion partners with mitogen-activated human b cells for the construction of ig-producing human b cell hybridomas. in a series of experiments using these lines and b cells from several tissue sources, approximatley 20% of the cultures plated were consistently positive for growth after hypoxanthine-aminopterin-thymidine selection and approximatley 30% of these synthesized significant new ig. a marked increase in ig secreti ... | 1982 | 6286839 |
| production of hepatitis b e antibody in epstein-barr virus-induced b lymphocyte cell lines. | b lymphocytes separated from anti-hbe-positive donors were established as lymphoblastoid cell lines by infection with ebv, but anti-hbe in the culture supernatant from such lymphoblastoid cell lines could not be detected. the lymphoblastoid cell lines were rosetted with hbe antigen-coupled srbc to prepare cells for the production of specific anti-hbe. antibody activity in the culture supernatant against rosette forming cells was detected by ria, id, and r-phi tests during the first 1 to 4 wk, bu ... | 1982 | 6286863 |
| organization of the epstein-barr virus dna molecule. ii. fine mapping of the boundaries of the internal repeat cluster of b95-8 and identification of additional small tandem repeats adjacent to the hr-1 deletion. | we used cloned bamhi fragments from epstein-barr virus strain b95-8 [ebv(b95-8)]dna to obtain detailed restriction maps of the region of the genome adjacent to the large internal repeat cluster. these maps together with the results of hybridization experiments using a 3.1-kilobase repeat probe defined more precisely the location of the injection between the internal repeat cluster and the flanking unique-sequence dna. on one side (ul), the repeat sequences extended 600 +/- 80 base pairs (bp) int ... | 1982 | 6286998 |
| filamentous structures associated with epstein-barr virus-infected cells. | after the onset of epstein-barr virus dna and protein synthesis 10 h after superinfection of raji cells (a cell line containing epstein-barr virus dna but not producing virus), filamentous structures 25 nm in diameter and 0.2 to 1.4 micrometers in length could be detected in the cell cytoplasm by electron microscopy. these structures banded in metrizamide gradients with viral dna and proteins, but at a density different from that of virions or nucleocapsids. these filaments, enriched in a 155,00 ... | 1982 | 6287004 |
| identification and isolation of the main component (gp350-gp220) of epstein-barr virus responsible for generating neutralizing antibodies in vivo. | the majority of hybridomas we have characterized against epstein-barr virions react with the major glycoproteins gp350 and gp220 (gp350/220). one of these antibodies, id4c-1, neutralizes virus infection in vitro. the presence of gp350/220 on the viral envelope could be confirmed directly by immunoelectron microscopy. we used lectin affinity (ricin) and immunoaffinity (id4c-1) to purify gp350/220 and show that this material is able to induce potent virus-neutralizing antibodies. absorption of fou ... | 1982 | 6287039 |
| persistence of transforming and nontransforming epstein-barr virus in high passages of p3hr-1 cell lines. | at least three laboratories have reported that the p3hr-1 line, which had originally produced transforming epstein-barr virus (ebv), now produces only the nontransforming variant. studies to determine whether these findings were universal or a consequence of specific cell lines or culture conditions were undertaken in p3hr-1 cultures of identical hla types from five sources. all of the ebv preparations derived from cell lines cultured at 32, 34, and 35 degrees c transformed cord blood lymphocyte ... | 1982 | 6287082 |
| epstein-barr virus-induced b-cell lymphoma after renal transplantation. | 1982 | 6287264 | |
| cryopreservation of human peripheral blood lymphoid cells reactive to allogeneic antigens, virus-transformed autologous cells and autologous leukemia cells in in vitro sensitization. | 1982 | 6287268 | |
| unique cell lines harbouring both epstein-barr virus and adult t-cell leukaemia virus, established from leukaemia patients. | members of three distinct classes of animal virus have been associated with naturally occurring neoplasias in man: epstein--barr virus (ebv), a dna virus belonging to herpesvirus group, papillomavirus, and a novel human rna (retro) virus, human t-cell leukemia virus or adult t-cell leukaemia (atl) virus. we have established seven continuous cell lines from atl patients and 0.1-7% of these cells consistently express atl-specific antigens (atla). ebv-associated nuclear antigen (ebna) is also found ... | 1982 | 6287295 |
| [leukocyte migration inhibition elicited by epstein-barr virus antigen. reaction potential in infectious mononucleosis as well as in states of immune deficiency and immunosuppression]. | 1982 | 6287380 | |
| [is there a relationship between epstein-barr virus and rheumatoid arthritis (author's transl)]. | antibodies directed against a nuclear antigen of lymphocytes infected by epstein-barr virus (rana antigen) have been detected in the sera of patients with rheumatoid arthritis, where they were present at higher levels than in the sera of controls. in vitro, the t lymphocytes of patients with rheumatoid arthritis seem to be incapable of controlling the multiplication of b lymphocytes induced by epstein-barr virus. the stimulated t lymphocytes produce more rheumatoid factors than normal lymphocyte ... | 1982 | 6287408 |
| [relationship between oral tissues and human tumor viruses (author's transl)]. | 1982 | 6287654 | |
| frequencies of specific antibody-producing cells for sheep red blood cells and for phosphorylcholine in human tonsillar b lymphocytes transformed by epstein-barr virus. | the frequencies of transformed b cells and of transformed specific antibody-producing cells in human tonsillar lymphocytes infected with epstein-barr virus (ebv) were determined by limiting dilution analyses. the latter was corrected by the former and the frequencies of specific antibody-producing cells in ebv-transformed b cell populations were obtained. the frequencies of specific b cells for sheep red blood cells were calculated to be one in 500 to 2,000 ebv-transformed tonsillar b cells obta ... | 1982 | 6287669 |
| high incidence of igg class of epstein-barr virus capsid antibody in indian patients of lepromatous leprosy. | low levels of epstein-barr virus capsid (ebvc) antibody of the igg class were detected in the sera of 19 of 23 (82.6%) lepromatous leprosy patients and six of 38 (16%) healthy controls. in contrast, heterophile antibody was found in only six of 43 (14%) lepromatous patients and three of 41 (7%) normal subjects. overlap of the two types of antibody occurred only in one normal serum. it is inferred that the presence of ebvc antibody against an unbiquitous virus in lepromatous patients who often su ... | 1982 | 6287684 |
| epstein-barr virus induction by a serum factor. i. induction and cooperation with additional inducers. | 1982 | 6287718 | |
| epstein-barr virus in cerebrospinal fluid during infectious mononucleosis encephalitis. | epstein-barr virus (ebv) was recovered from cerebrospinal cells (but not from cell-free fluid) of a patient with meningoencephalitis complicating infectious mononucleosis. this finding suggests that virus-altered b lymphocytes can invade the central nervous system and may play a direct role in the pathogenesis of neurologic sequelae of ebv infections. several mechanisms by which these cells can cause neurologic manifestations are discussed. | 1982 | 6287744 |
| presumable non-a, non-b hepatitis in hemodialysis. | hepatitis a and b markers, igm-anti-cmv and -ebv were determined in 154 dialysis patients, 118 relatives of patients and 42 members of the staff. episodes of elevated transaminases were investigated for presumable non-a, non-b hepatitis as well as secondary infections among relatives. --the cumulative frequency of hbv markers was 71% in center dialysis patients, 63% in home dialysis patients, 50% in staff and 19% in relatives. the frequency of hbv markers increased with duration of dialysis trea ... | 1982 | 6287760 |
| polyclonal b-cell activators in the study of the regulation of immunoglobulin synthesis in the human system. | 1982 | 6287820 | |
| acute sporadic non-a and non-b hepatitis in an urban community in new zealand. | acute sporadic non-a, non-b hepatitis is reported for the first time in new zealand. examination of sera from 94 patients with biochemical evidence of hepatitis showed that 26 (27%) had acute hepatitis b (hb), 22 (23%) had acute hepatitis a (ha) and 25 (26%) had acute non-a, non-b hepatitis (nanbh). nine (10%) patients had epstein-barr virus (ebv) associated hepatitis and one (1%) had cytomegalovirus (cmv) hepatitis. there were 11 (13%) patients with mixed infections; eight with ha plus hb, one ... | 1982 | 6287989 |
| frequencies of epstein-barr virus-inducible igm anti-igg b lymphocytes in normal children and children with juvenile rheumatoid arthritis. | the relative frequencies of igm antiigg autoantibody (rheumatoid factor) producing cells induced by the polyclonal b cell activator epstein-barr virus were measured in peripheral blood lymphocyte cultures of normal children and patients with juvenile rheumatoid arthritis. the frequencies of rheumatoid factor precursor b cells in normal children were lower than adults, but higher than neonates. the frequency increased with the age of the donor. in seronegative children with the systemic-onset or ... | 1982 | 6288055 |
| chromatin structure interferes with excision of abnormal bases from dna. | cell-free extracts of human lymphoblastoid cells nl3 excised almost all uracil residues from free dna with misincorporated dump, but only about half the uracil residues from nuclei, chromatin and reconstituted chromatin with dump-misincorporated dna. this difference in susceptibility to uracil-dna glycosylase of free and complexed dnas was similar to the difference in susceptibility of free and complexed methylated dnas to 3-methyladenine-dna glycosylase. methylated poly(da-dt) was also protecte ... | 1982 | 6288101 |
| elevated nad(p) glycohydrolase activity: a possible enzymatic marker for malignancy in burkitt's lymphoma cells. | a comparative analysis of enzymatic activities has been performed on 47 human continuous lymphoid lines: 22 tumors derived from burkitt's lymphoma lines, 6 other lymphomatous long-term cultures, and 19 nonmalignant ties determined on the cell extracts. 4 showed no significant differences between the various lines. they included adenosine diphosphoribose incorporation, glucose-6-phosphate dehydrogenase, cyclic-amp phosphodiesterase, and glutathione reductase. however, striking differences of acti ... | 1982 | 6288145 |
| lymphomatoid granulomatosis and epstein-barr virus. | a case report of a patient with lymphomatoid granulomatosis presenting initially as a reactivated epstein-barr virus infection is presented. epstein-barr virus is proposed in the possible role of establishing of an immunologically compromised state that may have set the stage for dissemination of this disease process. of interest is the fact that successful chemotherapeutic management of the disease was accomplished using prednisone and cyclophosphamide. furthermore, this clinical success was re ... | 1982 | 6288216 |
| chromosomal breakage and sister chromatid exchange in peripheral blood lymphocytes and lymphoblastoid cell lines in the x-linked lymphoproliferative syndrome. | the frequencies of chromosomal aberrations and sister chromatid exchange (sce) were measured in lymphoblastoid cell lines (lcls) and in phytohemagglutinin (pha)- and pokeweed mitogen (pwm)-stimulated lymphocytes from males with x-linked lymphoproliferative (xlp) syndrome, their obligate carrier mothers, and control subjects. we observed an increased frequency of chromosomal aberrations including increased polyploidy in lcls derived from families with xlp with time in culture. the sce rate in lc ... | 1982 | 6288230 |
| intervening effect of l-ascorbic acid on epstein-barr virus activation in human lymphoblastoid cells and its comparison with the effect of retinoic acid. | 1982 | 6288231 | |
| similarity of epstein-barr virus early polypeptides induced by various tumor promoters. | epstein-barr virus (ebv)-harboring non-producer raji cells were activated to express epstein-barr virus early antigen (ea) by the combined use of n-butyrate and various tumor promoters such as 12-o-tetradecanoyl phorbol-13-acetate (tpa), euphorbiaceae plant extracts and the toxic microbial metabolite, teleocidin. with regard to the structural differences among the inducing agents (promoters), the patterns of ebv early polypeptides were strikingly similar. a common pathway is probably involved in ... | 1982 | 6288232 |
| [comparison of efficiency of various methods of induction of epstein-barr virus antigens synthesis in human lymphoblastoid cell lines]. | 1982 | 6288272 | |
| epstein-barr virus (ebv)--lymphoid cell interactions. ii. the influence of the ebv replication cycle on natural killing and antibody-dependent cellular cytotoxicity against ebv-infected cells. | we investigated the influence of the epstein-barr virus (ebv) replication cycle on natural killing (nk) activity and antibody-dependent cellular cytotoxicity (adcc) against ebv-infected cells. peripheral blood lymphocytes from healthy ebv-seropositive and -seronegative donors were separated on ficoll-hypaque gradients and used as effector cells in the standard 51cr release assay to measure nk and adcc. ebv-genome positive raji and daudi cells superinfected with either the non-transforming p3hr-1 ... | 1982 | 6288292 |
| effect of different epstein-barr virus-determined antigens (ebna, ea, and vca) on the leukocyte migration of healthy donors and patients with infectious mononucleosis and certain immunodeficiencies. | 1982 | 6288294 | |
| immature b cells in fetal development and immunodeficiency: studies of igm, igg, iga and igd production in vitro using epstein-barr virus activation. | epstein-barr virus has been used as a b cell mitogen to explore the parallels between the b cells found in patients with hypogammaglobulinemia and the immature b cells in fetal tissues. peripheral blood lymphocytes from 29 cases of late onset hypogammaglobulinemia (common variable immunodeficiency) and from 10 cases of x-linked hypogammaglobulinemia were depleted of t lymphocytes and stimulated with virus in vitro. immunoglobulin production was measured over a 4-week culture period using inhibit ... | 1982 | 6288397 |
| the alpha 2 hs glycoprotein receptor on lymphocytes transformed by epstein--barr virus. | the alpha 2hs glycoprotein receptor from lymphocytes transformed by epstein--barr virus was isolated by affinity chromatography. the protein receptor has a monomer mr of 48 000, which is similar to the epstein--barr virus-determined nuclear antigen (ebna), and pi = 7.2. like ebna the 48 000 mr component in unfractionated labelled detergent solubilised cell supernatants also binds dna. these results may suggest some similarity between the alpha 2hs receptor and ebna. | 1982 | 6288468 |
| the pathogenesis of infectious mononucleosis. | 1982 | 6288544 | |
| cyclosporin a--usefulness, risks and mechanism of action. | 1982 | 6288546 | |
| interaction of epstein-barr virus with leukaemic b cells in vitro. ii. cell line establishment from prolymphocytic leukaemia and from waldenström's macroglobulinaemia. | leukaemic b-cell populations were prepared from six patients with high-count prolymphocytic leukaemia (pll) as well as from one patient with waldenström's macroglobulinaemia (wm) in frankly leukaemic phase, and their response to in vitro epstein-barr (eb) virus infection was monitored in terms of expression of the virus-associated nuclear antigen ebna and of virus-induced transformation to continuous cell lines. the individual leukaemic populations, tested on several occasions, gave reproducibly ... | 1982 | 6288575 |
| identification of the rpmi 8226 retrovirus and its dissemination as a significant contaminant of some widely used human and marmoset cell lines. | a retrovirus designated rpmi 8226v, isolated in 1973 from the human myeloma cell line rpmi 8226 has been characterized by competition radioimmunoassay (ria) for the major viral structural protein and by nucleic acid hybridization analysis using cdna of the virus. the virus is highly related to the squirrel monkey type d retrovirus, smrv. in the homologous ria using rabbit anti-rpmi 8226v and 125i-labelled p37 of rpmi 8226v, rpmi 8226v and smrv exhibited competition of 81% and 73% respectively. s ... | 1982 | 6288581 |
| complementation between infecting epstein-barr virus and intrinsic viral genomes in human lymphoid cell lines. | 13 human lymphoid cell lines previously characterized in terms of their intrinsic epstein-barr virus (ebv) genome content were infected or superinfected with ebv from the supernatant of p3hr-1 cells. the cell lines included 2 lymphoma cell lines that contain no detectable ebv genomes and 11 cell lines containing intrinsic ebv genomes. all the cell lines responded to ebv infection by expressing early antigen (ea). the number of ea-positive cells in the cultures was proportional to the viral conce ... | 1982 | 6288617 |
| simple assay method for susceptibility of human lymphocytes to epstein-barr virus infection. | on the basis of the sequential early events induced by epstein-barr virus (ebv), the infectivity of human lymphocytes to ebv was quantified as a plateau value of the maximum nuclear antigen (ebna) synthesis preceding cellular dna synthesis. the frequency of ebv-infectible cells was an average of 7.6% of unfractionated umbilical cord lymphocytes and was 4.9% in the counterparts from human adults. in the b-cell-enriched populations, the average plateau values were 29.2% in umbilical cord and 15.5% ... | 1982 | 6288618 |
| epstein-barr virus induction by a serum factor. purification of a high molecular weight protein that is responsible for induction. | serum contains a factor that induces epstein-barr virus antigens in latently infected human lymphoblastoid cell lines and that cooperates with chemical inducers. here we report the purification of a protein that is responsible for the effect. using ammonium sulphate precipitation, chromatography on deae-agarose and cellulose, molecular sieve chromatography on bio-gel a-5, and glycerol gradient centrifugation, the factor was enriched about 300,000-fold compared to calf serum. under neutral condit ... | 1982 | 6288682 |
| epstein-barr virus induction by a serum factor. characterization of the purified factor and the mechanism of its activation. | purified epstein-barr virus-inducing factor shows all the properties previously described for crude serum, i.e. inducing activity, cooperative activity with the inducers 12-o-tetradecanoyl-phorbol-13-acetate, 5'-iodo-2'-deoxyuridine, n-butyric acid, anti-igm, and the need to be activated by ph shock. a small subpopulation of untreated factor molecules is fully active, however. the factor is a protein that is characterized by considerable heat resistance and sensitivity to reducing agents. it doe ... | 1982 | 6288683 |
| detection of immunoglobulin m antibody to epstein-barr virus by use of an enzyme-labeled antigen. | immunoglobulin m (igm) antibodies to epstein-barr virus were detected by using microtiter plates coated with anti-mu-chain antiserum and enzyme-labeled epstein-barr virus antigen. optimum conditions for labeling were determined. the addition of unlabeled control antigen to the enzyme-labeled antigen was effective in reducing background reactions. rheumatoid factor no longer interfered. blocking of specific igm antibody by igg also was no longer observed. four different methods for the detection ... | 1982 | 6288765 |
| alteration of in vitro immunoglobulin secretion by amosite asbestos. | we investigated the in vitro effects of amosite asbestos on immunoglobulin (ig) secretion by human peripheral blood mononuclear leukocytes (mnl). concentrations of 100 to 300 micrograms/ml of amosite asbestos reduced the number of ig-secreting cells recovered from 6-day cultures of unstimulated mnl or mnl stimulated with epstein barr virus. by contrast, the ig secretory response to pokeweed mitogen was enhanced by 10 to 100 micrograms/ml concentrations of amosite asbestos; however, amosite asbes ... | 1982 | 6288799 |
| selective induction of autoantibody secretion in human bone marrow by epstein barr virus. | the bone marrow is an important site for b lymphocyte differentiation and antibody synthesis in animal and man. however, few experiments have examined directly its immunologic functions in humans. in the present experiments, we have induced bone marrow b lymphocytes from human donors with degenerative arthritis of varying ages to secrete two autoantibodies, igm and anti-igg (rheumatoid factor) and igm anti-human thyroglobulin (tg), by stimulation with the polyclonal b cell activator epstein barr ... | 1982 | 6288800 |
| the biology and chemistry of epstein-barr virus. | epstein-barr virus (ebv) is the human prototype of a family of closely related herpesviruses of old world primates. these agents probably evolved and spread among the old world primates since the divergence of apes from monkeys about 30 million years ago. although the dnas of the ebv family have no sequence homology to other herpesviruses, there are some features in common with other herpesviral genomes. ebv dna is unusual in having five tandem direct repeat elements which divide the genome into ... | 1982 | 6288806 |
| improved test for igm antibody to epstein-barr virus using an absorption step with staphylococcus aureus. | the determination of levels of igm antibody to epstein-barr virus (ebv) capsid antigen was improved by absorbing unfractionated test serum with staphylococcus aureus cells containing protein a. nonspecific background fluorescence was significantly decreased, thus facilitating the reading of slides and the detection of specific fluorescent reactions in serum with low levels of this igm antibody. moreover, the inclusion of the absorption step eliminated false-positive reactions caused by the prese ... | 1982 | 6288807 |
| [serological studies in early stage of nasopharyngeal carcinoma ]. | 1982 | 6288834 | |
| acute autonomic and sensory neuropathy associated with elevated epstein-barr virus antibody titre. | 1982 | 6288883 | |
| hla-d typing using epstein-barr virus-induced lymphoblastoid cell lines. | we examined whether epstein-barr virus-induced lymphoblastoid cell lines, established from homozygous typing cells (htc-ebv-lcl), could be substituted for normal homozygous typing cells (htc) in hla-d typing. when using ebv-lcl as stimulators in one-way mlr, the stimulator: responder ratio was found to be most important. at a ratio of 1:10, the autologous mlr between ebv-lcl and pbl from the same donors exhibited low double normalized values (dnv) which could be distinguished from those of homol ... | 1982 | 6288983 |
| cerebrospinal fluid markers in african burkitt's lymphoma with central nervous system involvement. | several cerebrospinal fluid markers were found to be elevated in burkitt's lymphoma patients with central nervous system (cns) involvement. antibody levels to the virus capsid antigen of the epstein-barr virus and to the brain cell antigens myelin and cerebroside were elevated during active cns disease. immune complexes were present in levels above 100 micrograms/ml in most patients with cns involvement but tended to be low or negative in patients without cns disease. oligoclonal igg bands were ... | 1982 | 6288991 |
| epstein-barr virus induced proteins v: comparison of ebv-specific polypeptides from different virus strains. | ebv-associated polypeptides induced in different epstein-barr virus (ebv)-producing cell lines by the tumor promotor tpa, and from purified ebv particles derived from the same lines were radioactivity labeled and analyzed by immunoprecipitation with human vca+ma+ sera. in virus-producing cells no significant differences in the molecular weight of 35s-methionine-labeled ebv-associated polypeptide patterns could be observed. the analysis 125i-labeled polypeptides from purified virus particles of f ... | 1982 | 6289060 |
| persistent viral infections of man. | 1982 | 6289088 | |
| cell cloning as a tool to analyze the immune response. | long-term lines of cloned, immunocompetent, nontransformed lymphocytes have the potential to allow the direct examination of many of the key aspects of lymphocyte activation and regulation. we describe here techniques for the long-term propagation of nontransformed human and mouse b-lymphocytes. these lines have the shared characteristics in that they bear membrane ig, that their growth depends on the presence of appropriate growth factors, and that, when properly stimulated, they secrete ig. we ... | 1982 | 6289105 |
| dimer-size dna circles in a leukemic cell immortalized with the epstein-barr virus. | the intracellular state of the 30 viral genome equivalents of epstein-barr virus (ebv) dna carried in latent form by the cii cell line, established from a chronic lymphocytic leukemia patient, has been partially characterized. the cii line, which has markers confirming its tumor origin, extends the analysis of the intracellular state of ebv dna to include other, non-burkitt lymphoid tumor cells. monomer-size, free, circular ebv genomes, the major intracellular viral dna species in other ebv-tran ... | 1982 | 6289318 |
| phenotypic manifestations of gaucher disease: clinical features in 48 biochemically verified type 1 patients and comment on type 2 patients. | 1982 | 6289358 | |
| [contribution to the true non-african burkitt's lymphoma (author's transl)]. | it is reported on a 6-year-old middle european boy suffering from a nasopharynx tumor with involvement of a submandibular lymph node. the tumor was classified by morphologic, serologic and virologic data as a true or epstein-barr virus-associated burkitt's lymphoma, resp., which has been, up to now, seldom seen outside africa. the patient died 3 months after diagnosis. autopsy has shown tumor involvement of nasopharynx and soft tissues in the left neck region, several lymph nodes, kidneys, liver ... | 1982 | 6283764 |
| epstein-barr virus-induced diseases in boys with the x-linked lymphoproliferative syndrome (xlp): update on studies of the registry. | analyses of 100 subjects with the x-linked lymphoproliferative syndrome (xlp) in 25 kindreds revealed four major interrelated phenotypes: infectious mononucleosis, malignant b-cell lymphoma, aplastic anemia, and hypogammaglobulinemia. eighty-one of the patients died. two male subjects were asymptomatic but showed immunodeficiency to epstein-barr virus (ebv). seventy-five subjects had the infectious mononucleosis phenotype and concurrently, 17 subjects of this group had aplastic anemia. all subje ... | 1982 | 6283885 |
| disseminated kaposi's sarcoma in homosexual men. | nineteen cases from an epidemic of disseminated kaposi's sarcoma in homosexual men were studied by clinical, virologic, immunologic, and genetic methods. the patients were all male homosexuals ranging in age from 29 to 52 years, with histories of multiple sexually transmitted diseases and exposure to both prescription and recreational drugs. sites of disease included skin (16 of 19 patients), lymph nodes (13 patients), gastrointestinal tract (12 patients), spleen (three patients), and lung (one ... | 1982 | 6283973 |
| infectious mononucleosis in an adult progressing to fatal immunoblastic lymphoma. | we report a case of infectious mononucleosis progressing to fatal immunoblastic lymphoma. the patient, a 44-year-old man who may have had an immunoregulatory defect, failed to have an appropriate t-cell response to his epstein-barr (eb) viral infection. his active eb viral infection was manifest by seroconversion of igm-viral capsid antibody and a greater than four fold rise in igg-viral capsid antibody. also, he transmitted his eb viral infection to his wife who became ill 1 month after his dea ... | 1982 | 6283976 |
| surface glycoproteins of malignant human leukocytes. | 1982 | 6284215 | |
| [anti-complement immunoperoxidase technique in the determination of ebna antibody level in patients with nasopharyngeal carcinoma (author's transl)]. | 1982 | 6284464 | |
| analysis of dr-like molecules on a marmoset epstein-barr virus-induced cell line using a monomorphic anti-human hla-dr monoclonal antibody. | mouse anti-hla d region-related (dr) monoclonal antibodies have been found to cross-react with peripheral blood leukocytes from one primate species, the common marmoset (callithrix jacchus). immunoprecipitates of radioactively labeled cells extracted from a marmoset epstein-barr virus-induced cell line were analyzed by one- and two-dimensional gel electrophoresis and compared with the dr antigens of a human lymphoblastoid b cell line. two chains of estimated molecular weights of 34 000 (alpha) a ... | 1982 | 6284521 |
| the role of the human major histocompatibility complex in cytotoxic t-cell responses to virus-infected cells. | the human major histocompatibility complex (hla) has been demonstrated to play two roles in the generation and expression of cytotoxic t-lymphocyte responses to virus-infected cells: (1) cytotoxic t cells can only recognize viral antigens in conjunction with antigens encoded by hla-a and -b genes; and (2) hla-linked genes may control the capacity to generate t-cell responses to a given virus or to virus in conjunction with particular self hla-a and -b antigens. analysis of t-cell responses gener ... | 1982 | 6284788 |
| time of synthesis and cellular location of epstein-barr virus-specified polypeptides radioimmunoprecipitated from iododeoxyuridine-induced burkitt lymphoma/somatic cell hybrids. | the burkitt lymphoma/somatic cell hybrid d98/hr-1 contains repressed epstein-barr virus (ebv) genomes which can be induced by 5-iodo-2'-deoxyuridine (idurd) to express the ebv early antigen (ea) and the virus capsid antigen (vca). nuclear and cytoplasmic fractions prepared from uninduced, partially induced and fully induced hybrid cells were radioimmunoprecipitated with human sera having high antibody titres against ea and vca. three polypeptides, with mol. wt. 127 x 10(3), 115 x 10(3) and 84 x ... | 1982 | 6284867 |
| gianotti disease or gianotti-crosti syndrome? | 1982 | 6284901 | |
| a possible association between hepatitis-b antigen-negative infantile papular acrodermatitis and epstein-barr virus infection. | 1982 | 6284902 | |
| effect of the arginine analog canavanine on the synthesis of epstein-barr virus-induced proteins in superinfected raji cells. | the addition of canavanine to cultures of superinfected raji cells in the absence of arginine prevented the appearance of early antigens as defined by immunofluorescent staining. addition of various amounts of arginine permitted the identification of at least three groups of proteins, each responding differently to the various concentrations of arginine-canavanine. | 1982 | 6284966 |
| dna of herpesvirus pan, a third member of the epstein-barr virus-herpesvirus papio group. | the dna of herpesvirus pan, a primate b-lymphotropic herpesvirus, shares about 40% well-conserved sequence relatedness with epstein-barr virus (ebv) and herpesvirus papio dnas. labeled cloned fragments from the ebv recombinant dna library were cross hybridized to blots of ecori, xbai, and bamhi restriction endonuclease fragments of herpesvirus pan dna to identify and map homologous sequences in the herpesvirus pan genome. regions of colinear homology were demonstrated between 6 x 10(6) daltons a ... | 1982 | 6284982 |
| [immunoglobulin genes and b cell differentiation, with special reference to neoplastic changes of b cells]. | 1982 | 6285035 | |
| eb-virus and rheumatoid arthritis: new insights into their interrelation. | 1982 | 6285090 | |
| determination of specific iga antibodies to varicella zoster virus by immunoperoxidase assay. | an indirect peroxidase technique was developed for determination of iga antibodies to varicella zoster virus (vzv). the antigen consisted of acetone-fixed trypsinised vzv-infected cells. rabbit antihuman iga peroxidase conjugate was used to detect human iga antibodies bound to viral antigen. in parallel igg antibodies to vzv were determined by an immunoperoxidase antibody to membrane antigen (ipama) technique. varicella zoster virus iga antibodies were detected in all five varicella and seven zo ... | 1982 | 6282941 |
| [malignant lymphoma of the nasopharynx of non-burkitt's type--a case report of a patient with positive epstein-barr virus-associated nuclear antigen (ebna) (author's transl)]. | 1982 | 6283047 | |
| post-viral haemophagocytic syndrome. | 1982 | 6283079 | |
| two large virion envelope glycoproteins mediate epstein-barr virus binding to receptor-positive cells. | the four major epstein-barr virion envelope components were separated by column chromatography and reconstituted into artificial liposomes. these liposomes were tested for their ability to bind selectively to epstein-barr virus receptor-positive cells. only when the two high-molecular-weight glycoproteins, ve1 and ve2, were present together was a stable binding complex formed. the addition of the other virion envelope components did not increase the levels of binding. this binding was inhibited ... | 1982 | 6283120 |
| epstein-barr virus-lymphoid cell interactions. iii. effect of concanavalin a and saccharides on epstein-barr virus penetration. | to study some aspects of epstein-barr virus (ebv) penetration into target cells, the effect of concanavalin a (cona) and various saccharides on virus infectivity and cell susceptibility to ebv infection was examined. cona treatment of the target cells, ebv, or ebv-cell complexes was found to inhibit virus antigen expression. several control experiments with alpha-d-methyl-mannoside elution of cona, removal of nonfused ebv particles from the cell surface by trypsin treatment, and addition of cona ... | 1982 | 6283164 |
| epstein-barr virus increases the proliferative response and the generation of suppressor and cytotoxic t-cell functions in autologous mixed lymphocyte reaction. | epstein-barr virus (ebv) infection of stimulator cells significantly increased the proliferative response of t cells in the autologous mixed lymphocyte reaction (amlr). the addition of a monoclonal anti-hla-dr antibody to amlr cultures in which either ebv-infected or non-infected non-t cells were used as stimulator cells strongly inhibited the proliferative response irrespective of the presence of ebv. it is concluded that ebv does not by itself activate the responding cells and that hla-dr anti ... | 1982 | 6280268 |
| morphologic observations of contact-induced lysis of ebv-infected b lymphocytes by autologous hand mirror t cells. | to study the possible immunologic role of hand mirror lymphocytes (hml) in the control of epstein-barr virus (ebv)-infected b lymphocytes in patients with infectious mononucleosis (im), we studied the in vitro interactions of these cells by time-lapse video light microscopy. peripheral blood t lymphocytes isolated from three patients in the early recovery phase of im were mixed with their autologous ebv-infected b cells. motile lymphocytes with their characteristic hand mirror shape were observe ... | 1982 | 6280495 |
| epstein-barr virus and a unilateral neck mass. | although epstein-barr infectious mononucleosis (im) might be considered in the differential diagnosis of multiple neck masses in a young adult, single large masses are not usually associated with im. this patient did have a solitary mass. despite lacking other signs of im, he did have a positive heterophil-agglutination titer and reactive lymphocytes. other serologic tests can be of value in confirming the diagnosis, especially if the heterophil titer and reactive lymphocytes are absent. these t ... | 1982 | 6280658 |
| oropharyngeal epstein-barr virus excretion in rheumatoid arthritis. | we hypothesized that the defective cellular regulation of epstein-barr virus (ebv) in rheumatoid arthritis (ra) might be reflected in an increased rate of oropharyngeal virus excretion, but we found that the prevalence of excretion in 45 ra patients (22%) did not differ from 45 age- and sex-matched non-ra patients (24%) who were taking similar medications. increased excretion rates correlated with corticosteroid therapy and male sex, but not with age or serum levels of ebv antibodies. | 1982 | 6280734 |
| characterization of transformed cells and tumors by proton nuclear magnetic resonance spectroscopy. | cultured acute lymphoblastic leukemic cells give a well-resolved proton nuclear magnetic resonance spectrum characteristic of isolated plasma membranes. we demonstrate that the signals, in the spectrum of whole cells, arise predominantly from the plasma membrane and that cells transformed by pokeweed mitogen have membranes which are significantly less rigid than are normal human peripheral blood lymphocytes. normal thymus, malignant thymus, and a leukemic t-cell line have been compared by proton ... | 1982 | 6280854 |
| characteristics of hodgkin's disease-derived cell lines. | in the last 3 years we were able to establish five long-term in vitro cell cultures from biopsy specimens taken preterminally from four patients with histologically proven hodgkin's disease (nodular sclerosing type, clinical stage ivb). four of the lines are continuously proliferating in vitro; one culture stopped growth for unknown reasons after 7 months. when culture conditions were modulated, the first culture, l 428, gave rise to two sublines: l 428 ks, after adaptation to calf serum, and l ... | 1982 | 6280862 |
| immunoglobulin properties of epstein-barr virus transformed human umbilical cord and adult peripheral blood lymphocytes. | 1982 | 6280881 | |
| placental and fetal pathology in infectious mononucleosis. a possible indicator for epstein-barr virus teratogenicity. | placentae and membranes were studied microscopically in five cases of pregnancy interruption due to maternal infectious mononucleosis in the first 2 months of pregnancy. this viral disease, caused by epstein-barr virus and apparently rare in pregnancy, induced specific placental lesions. in the decidua, these lesions were manifested by perivasculitis and necrotizing deciduitis. the membranes exhibited slight to moderate chorionitis. in the villi, endovasculitis, perivasculitis, and occasional va ... | 1982 | 6280944 |
| human monoclonal autoimmune antibody produced in vitro: rheumatoid factor generated by epstein-barr virus-transformed cell line. | the infection of selected lymphocytes from a rheumatoid arthritis patient with epstein-barr virus resulted in an immortalized cell line that secretes a monoclonal rheumatoid factor (rf). the cloned line has been growing for more than 24 months, and constantly produces a monoclonal igm, lambda, 19s, rf (1-2 micrograms/ml/106 cells). the rf agglutinates human and rabbit igg (but not igm) and also protein a-coated erythrocytes, but fails to do so to mouse, goat and swine igg-coated erythrocytes. wh ... | 1982 | 6281026 |
| insulin binding to cultured b- and t-lymphocytes. | insulin receptors have been demonstrated on activated but not resting t-lymphocytes. we compared insulin binding to several t- and b-cell tissue culture lines transformed by either radiation or lymphotrophic herpes virus. two t-cell lines derived from mouse lymphomas, one t-cell derived from the cotton top marmoset and one mouse b-cell line bound, at most, 3% of a tracer dose of [125i]insulin. in contrast, positive b-cell line (1605 l) derived from a leukemic cotton top marmoset, bound 40% of tr ... | 1982 | 6281177 |
| differential cyst(e)ine requirements in human t and b lymphoblastoid cell lines. | in an effort to find exploitable metabolic differences between human t and b lymphoblasts, we have compared the ability of lymphocytes of varying phenotype to grow in cystine-deficient medium. only 6 of 12 human lymphoblastoid cell lines tested were able to utilize homocysteine thiolactone or cystathionine in place of cystine for growth. this difference in growth requirements was unrelated to the rate of cell division, the presence of epstein-barr viral genetic material, or whether or not the ce ... | 1982 | 6281197 |
| deficient activity of receptor-cyclase coupling protein is transformed lymphoblasts of patients with pseudohypoparathyroidism, type i. | erythrocytes of many patients with pseudohypoparathyroidism, type 1 (php-i) exhibit quantitatively reduced activity of the n protein, the guanine nucleotide-binding regulatory component of adenylate cyclase. we have designated this group of patients php-ia to distinguish them from php-ib patients, in whom erythrocyte n activity is quantitatively normal. in virus-transformed lymphoblasts of three normal, three php-ia, and two php-ib subjects, we compared n and adenylate cyclase activities, as wel ... | 1982 | 6281299 |
| characterization of the major epstein-barr virus-specific rna in burkitt lymphoma-derived cells. | cytoplasmic rna prepared from five lymphoid cell lines and a burkitt lymphoma biopsy was radioactively labeled in vitro and hybridized to cloned ecori restriction endonuclease fragments of b95-8 epstein-barr virus dna. the results confirmed that the most abundant cytoplasmic rna species in such cells is specified by a small region of the genome defined by the ecori j fragment. detailed mapping experiments precisely localized these transcripts within the sequence of the rightmost one-third of the ... | 1982 | 6281455 |
| use of fluoresceinated epstein-barr virus to study epstein-barr virus-lymphoid cell interactions. | as a direct approach to visualize epstein-barr virus (ebv) binding to its cellular receptors and to learn more on the nature of this binding, virus preparations were conjugated to fluorescein isothiocyanate and used to detect ebv receptors on lymphoid cells. different enzymatic and chemical treatments were also applied either to the virus or to target cells or to both, and the effect of these treatments on virus binding was then examined. the results obtained show that: (i) ebv can be fluorescei ... | 1982 | 6281475 |
| induction of epstein-barr virus nuclear antigen and dna synthesis in a human epithelial cell line after epstein-barr virus infection. | the association of epstein-barr virus (ebv) with nasopharyngeal carcinoma is supported by the presence of ebv genomes in the epithelial elements of the tumor and by elevated antibody titers to ebv-specific antigens in the patients; the levels of these titers are related to the clinical course of the disease. however, since most laboratory data suggest that ebv is a b-lymphotropic virus, it is unclear how the virus becomes associated with the epithelial elements of the nasopharynx. the purpose of ... | 1982 | 6281479 |
| [lymphadenitis caused by epstein-barr virus and cytomegalovirus]. | 1982 | 6281686 | |
| inverted repeat nucleotide sequences in the genomes of marek disease virus and the herpesvirus of the turkey. | the dnas of two herpesvirus, the oncogenic marek disease virus and the serologically related herpesvirus of the turkey, were studied by electron microscopy. on the basis of fold-back molecules observed in single-stranded dna from both viruses, structures have been derived from the overall nucleotide sequence arrangement in their genomes. although differing in molecular weight, the genomes of marek disease virus and turkey herpesvirus are both constructed according to the same plan--two regions o ... | 1982 | 6281774 |
| cell surface properties of hla antigens on epstein-barr virus-transformed cell lines. | a number of monoclonal antibodies have been used to investigate the distributions and rates of lateral motion of the hla-a,b, and-dr antigens on several epstein--barr virus-transformed b-cell lines. the lateral diffusion coefficients (d) of fluorescein conjugates of the monoclonal antibodies bound to the cell surface were determined by fluorescence recovery after pattern photobleaching. ds of hla-a and-b were found to be comparable and of the order of 10(-9) to 10(-10) cm2/sec for each of the se ... | 1982 | 6281776 |
| analysis of epstein-barr virus (ebv) of p3hr-1: isolation of ebv with ebna induction ability in human cord lymphocytes and without ea induction ability in raji cells. | 1982 | 6281981 |