Publications

heritability of infection-induced humoral antibacterial activity in the immune response of the mosquito aedes aegypti.		1996	8887345
a search for components in human body odour that attract females of aedes aegypti.	in a new type of wind tunnel, mosquitoes fly upwind towards host odour sources and towards human skin wash extracts obtained by rubbing the skin with a pad soaked in ethanol. we used this behavioural response as a bioassay to identify attractants in liquid chromatography fractions of such extracts. l-lactic acid is a major constituent of skin wash extracts and it is a necessary component for the extract's effectiveness. as a single stimulus, however, l-lactic acid is only slightly effective. thi ...	1996	8894295
sensory aspects of host location in mosquitoes.	visual, thermal and olfactory stimuli all contribute to blood meal host location in mosquitoes but olfaction is probably the dominant sensory modality used for this purpose. much attention has been devoted to the l-lactic acid receptor because it is well characterized and because its sensitivity is a major determinant of host responsiveness in the anautogenous species aedes aegypti. studies employing statistical analysis of close to 500 single unit recordings and the scanning electron microscope ...	1996	8894299
responses of antennal olfactory receptors in the yellow fever mosquito aedes aegypti to human body odours.	recent behavioural studies have demonstrated that human body odours which female aedes aegypti find attractive exert their effects as complex mixtures of synergistically acting components. we have attempted to clarify the sensory mechanisms underlying the perception of these complex host odours by studying the responses of sensory cells underneath the a3-type sensilla of the mosquito antenna to both a human skin wash extract and the extract's active chromatographic fractions. the reaction patter ...	1996	8894302
inter- and intraspecific variation in resistance to desiccation by adult aedes (stegomyia) spp. (diptera: culicidae) from indonesia.	desiccation survival times of adult mosquitoes were studied for yellow fever mosquito, aedes aegypti (l.) (6 strains), asian tiger mosquito, ae. albopictus (skuse) (5 strains), and ae. paullusi stone & farmer (1 strain) colonized from south sulawesi, indonesia. at both 90 and 70% rh, ae. aegypti males and females outlived the other species. the forest species ae. paullusi was least resistant to desiccation. strains of ae. aegypti and ae. albopictus from urban areas were more resistant to desicca ...	1996	8906905
occurrence of aedes (stegomyia) spp. mosquitoes (diptera: culicidae) in halmahela villages, indonesia.	aedes (stegomyia) spp. mosquitoes breeding in containers in and around human houses were surveyed in halmahela villages, the north moluccas, indonesia. adults reared from larvae found in containers and those reared from eggs collected by ovitraps revealed the dominance of aedes scutellaris (walker) in residential areas. breeding of aedes aegypti (l.) and aedes albopictus (skuse) was confirmed in a few coastal villages with high human densities.	1996	8906924
attraction and trapping of aedes aegypti (diptera: culicidae) with host odors in the laboratory.	volatile constituents of mouse odor were eluted from an adsorbent after collection within a closed-air system, and offered to host-seeking yellowfever mosquitoes, aedes aegypti (l.), in the laboratory. gas chromatographic analysis showed that individual mouse odors varied in blend, both quantitatively and qualitatively. in a 2-choice bioassay, sticky traps baited with individual mouse odors caught more female mosquitoes than controls (69% caught on baited traps) in all cases. when 2 traps baited ...	1996	8906926
identification and characterization of differentially expressed cdnas of the vector mosquito, anopheles gambiae.	the isolation and study of anopheles gambiae genes that are differentially expressed in development, notably in tissues associated with the maturation and transmission of the malaria parasite, is important for the elucidation of basic molecular mechanisms underlying vector-parasite interactions. we have used the differential display technique to screen for mrnas specifically expressed in adult males, females, and midgut tissues of blood-fed and unfed females. we also screened for mrnas specifica ...	1996	8917545
[ecology of aedes aegypti in africa and asia].	aedes aegypti is originated from africa and expanded around the tropical world with a pantropical distribution in 1930. because of mosquito control, this extension decreased, but is still very important and threatening because of disease transmission. eggs of ae. aegypti can survive unfavorable conditions. larvae and pupae of ae. aegypti breed into natural or artificial containers, the last being the most important. artificial breeding-sites are mostly water storage containers and discarded cont ...	1996	8924766
[the risk of urban yellow fever outbreaks in brazil by dengue vectors. aedes aegypti and aedes albopictus].	urban yellow fever (yf) epidemics have disappeared from brazil since about 50 years, but a selvatic cycle still exist. in many states, cases are more or less numerous each year. ae. aegypti was eradicated in 1954, re-appeared temporarily in 1967, and then definitively in 1976-1977. ae. aegypti is a vector of yellow few (yf), but also of dengue, whose first cases were reported in 1982. today, dengue is endemic in many regions. a second flavivirus vector, aedes albopictus is present since about te ...	1996	8924767
[aedes aegypti in french guiana. some aspects of history, general ecology and vertical transmission of the dengue virus].	aedes aegypti is suspected to be present in the country since the late 18th century, and was responsible of urban yellow fever epidemics in the last century. this mosquito was identified for the first time in french guiana in 1902. more recently, in 1940, an eradication campaign started and aedes aegypti was eradicated between 1950 and 1963, date of the reinfestation. during the past 30 years, some dengue outbreaks occurred every 2 to 6 years, and the first dengue haemorrhagic fever epidemic spr ...	1996	8924768
[prediction and prevention of dengue epidemics].	prediction and prevention of dengue epidemics are based on informations gathered about the mosquito vector species, the dengue types transmitted, the vertebrate hosts and their environment. although aedes aegypti is the most important vector, other aedes may also propagate the dengue viruses. the populations of vector mosquitoes are evaluated through several indices: breteau and/or positive house index, number of indoor resting aedes females, etc.... the four dengue types can replicate in verteb ...	1996	8924770
[dengue and its vectors in brazil].	history of dengue in brazil is covered from the first citations in the xixth century to the great outbreaks of the last ten years. den-1 and den-4 viruses have been isolated for the first time in 1982 during an epidemic in boa vista, roraima state. in 1986-1987, epidemics of dengue type 1 covered an extended area from rio de janeiro/sào paulo states to the north east states of brazil. during 1990-1991, dengue type 2 epidemics have been notified in the south east (rio de janeiro/são paulo) and in ...	1996	8924771
[control of mosquitos in martinique. a collective public health action and improvement of the quality of life].	aedes aegypti control program in martinique started en 1968. the aedes aegypti major breeding sites are associated with flowers vases, drums, used tyres, artificial containers and watering places. however, culex quinquefasciatus is the main nuisance mosquito in all the island. this species is unpleasant, especially in urban areas. over 200 culex quinquefasciatus bites per person per night have been observed in several localities of the island. culex quinquefasciatus breeding sites are mostly ass ...	1996	8924774
[anti-aedes aegypti campaign in french guiana].	the history of vector control in french guiana started in 1947 when a small team was recruited for that purpose. in 1949, the first ddt treatments were implemented and aedes aegypti could not be found until 1960. between 1966 and 1972 an eradication campaign was carried out which resulted in the elimination of ae. aegypti from all the department except the city of cayenne. malathion and orthodibrom were used as adulticides and temephos (abate) was sprayed against the larvae. nevertheless, in 198 ...	1996	8924775
[epidemiology of arbovirus diseases: use and value of physiologic age determination of female mosquito vectors].	the physiological age of yellow fever aedes females in africa was studied during four years, from 1988 to 1992. we used a method, according to polovodova's method, which looks for the "yellow body" under natural light. those yellow bodies exist in the old females, the "parous" ones, and not in the young females, the "nulliparous" ones. we present some results to illustrate the interest of studying the physiological age of mosquitoes in the epidemiology of the arboviral diseases. the transmission ...	1996	8924776
[control of aedes aegypti in martinique. contribution of entomology studies].	the dengue prevention activities in martinique are based on: entomological surveillance (including the use of insecticide in all parts of the island). epidemiological (clinical, serological and virological) surveillance. health education (radio, tv, exhibitions, talks in primaries and secondaries schools, in associations,...). the main breeding sites of aedes aegypti are maintained by human practices: flowers vases, containers, used tyres, waste... the role of those human practices in the standi ...	1996	8924777
[new epidemiological aspects of dengue].	since the 1940s, the dengue epidemics occur more and more often in the pacific islands with an increased severity. for example, in new caledonia, outbreaks of dengue-like diseases have been reported since the end of the last century but the first epidemic due to an identified virus occurred in 1942-1943 and was caused by the den-1 type. the next, due to the den-2 type, was reported thirty years later, in 1972-1973. after that, three outbreaks burst between 1975 and 1990, caused successively by d ...	1996	8924778
[the situation of dengue in the world].	the situation of dengue in the world can be summarized as follows: 2,5 billions persons at risk, 60 millions cases per year, and 30 000 deaths per year. the four dengue serotypes, den-1, den-2, den-3 and den-4 are nearly human-specific. clinical symptoms of dengue vary from unapparent infection, mild febrile manifestations to hemorrhagic forms with or without shock syndrome. dengue viruses are transmitted by aedes mosquitoes, the most important vectors being aedes aegypti and aedes albopictus. t ...	1996	8924780
[dengue in the americas].	dengue fever is endemic from argentina to mexico, where serotypes 1, 2 and 4 are in wide circulation. after an absence of almost 20 years, serotype 3 arrived in central america in late 1994. over the past decade dengue has become a major public health problem in many of these countries, with an increase in the prevalence of dengue haemorrhagic fever and in the number of fatal cases. the situation is not new; there is evidence of a least eight pandemics from the early 19th century until the initi ...	1996	8924781
[dengue in french guiana. history and present status].	the evolution of dengue in french guiana has the same trend as in most tropical american countries, with emergence of dengue hemorrhagic fever (dhf) and endemicity of the disease. during the 1940's, several dengue-like outbreaks were reported from french guiana. then, the only dengue vector, aedes aegypti, was eradicated between 1950 and 1963, when it started to reinfest the country. by the end of the 1960's, new dengue epidemics with serological confirmation were described. after 1970, the firs ...	1996	8924782
apyrase and alpha-glucosidase in the salivary glands of aedes albopictus.	an apyrase and an alpha-glucosidase were detected in the salivary glands extracts of adult aedes albopictus. the apyrase is a 61,000 da secreted protein that hydrolyses atp and adp. this protein is synthe-sized in adults and is preferentially accumulated in the distal lateral lobes of the female salivary glands. the alpha-glucosidase is a secreted 67,000 da protein. this enzyme is synthesized during adult life and accumulated in the proximal-lateral lobes of both males and females. the results a ...	1996	8925436
dengue fever at the u.s.-mexico border, 1995-1996.	dengue is a mosquito-transmitted acute disease caused by any of four virus serotypes (den-1, den-2, den-3 and den-4) and is characterized by acute manifestations that can include fever, headache, myalgia, arthralgia, rash, nausea, and vomiting. on august 25, 1995, public health authorities in mexico notified the texas department of health (tdh) of an ongoing outbreak of dengue fever in the state of tamaulipas, which borders south texas. because of the year-round presence of the aedes aegypti mos ...	1996	8927003
pathogenicity of the aedes albopictus parvovirus (aapv), a denso-like virus, for aedes aegypti mosquitoes.	aapv, a denso-like virus isolated from a c6/36 clone of the aedes albopictus cell line, proved to be very pathogenic for aedes aegypti first and third instar larvae following per os infection. the mortality reached 90% in 10 days for larvae infected at the first instar. several factors, such as temperature, larval density and stage, and duration of contact with infectious particles, influenced infection. the virus titer in females surviving infection at the third larval instar reached 10(8) tcid ...	1996	8931366
peritrophic matrix proteins of anopheles gambiae and aedes aegypti.	little is known about the composition and function of the mosquito peritrophic matrix (pm), a physical barrier that pathogens must traverse to complete their life cycles. anopheles gambiae and aedes aegypti pm proteins induced by blood or by a protein-free meal have been characterized by the use of 2-d gel electrophoresis and lectin-binding affinity assays. more than forty proteins have been identified in both species. over half of the pm proteins of both mosquitoes migrate identically. many pm ...	1996	8933177
changes in ribosomal protein rpl8 mrna during the reproductive cycle of the mosquito, aedes aegypti.	changes in the abundance of ribosomal protein rpl8 mrna were compared with 18s rrna and vitellogenin mrna in fat body from adult female aedes aegypti during the reproductive cycle. levels of rpl8 mrna began to increase within 2 h after adult eclosion, peaked at about 24 h post-eclosion, and remained high throughout the next 48 h. during this same period, rrna abundance increased about 2-fold. after the bloodmeal, levels of rpl8 mrna gradually decreased over the next 48 h, while rrna levels incre ...	1996	8969467
disposable containers as larval habitats for aedes aegypti in a city with regular refuse collection: a study in marília, são paulo state, brazil.	in marília, brazil, refuse is collected at least every other day, yet non-useful, non-returnable containers such as cans, plastic bottles and tires account for almost half of the container habitats found positive for the aedes aegypti mosquito. a study was therefore conducted to investigate why these containers exist despite regular refuse collection and a high level of awareness of dengue prevention, and how the control program could most effectively respond. differing community perceptions as ...	1996	8971274
[the yeast flora of blood-sucking mosquitoes].	yeast flora composition has been studied in natural populations of blood-sucking mosquitoes of genera aedes. anopheles and culex dwelling in the territory of ukraine. the authors have distinguished 18 yeast species belonging to 7 genera. species candida famata, c. (yarrowia) lipolytica as well as the yeast of genera rhodotorula and cryptococcus dominate on the larvae and imago of mosquitoes. the distinguished strains are the components of normal microflora of blood-sucking mosquitoes and take no ...	1996	8983519
the effect of chitin synthesis inhibitors on the development of brugia malayi in aedes aegypti.	two chitin synthesis inhibitors (csis) viz., triflumuron and hexaflumuron interfere++ with the development of brugia malayi in aedes aegypti (a black-eyed liverpool strain). the development of b. malayi was slow in both the treated populations and the infection rate, infectivity rate and l3 load per mosquito decreased significantly (p < 0.001) in comparison with untreated controls. hexaflumuron was found to be more inhibiting than triflumuron.	1996	8984113
in vivo effect of a natural aedes aegypti defensin on brugia pahangi development.		1996	8994145
nad(p)h-dependent production of oxygen reactive species by the salivary glands of the mosquito anopheles albimanus.	salivary gland homogenates of the adult female mosquito anopheles albimanus, but not those of aedes aegypti, induced light production in the presence of nadph and luminol, indicating a nadph oxidase activity producing reactive oxygen species (superoxide anion) by the anopheline salivary homogenate. superoxide production by the anopheline salivary homogenate was also confirmed by the nadph-dependent, superoxide dismutase inhibitable, reduction of cytochrome c. the nadph oxidase reaction measured ...	1996	8995793
[description of the colonization of aedes albopictus (diptera: culicidae) in the region of saõ josé do rio preto, sp, 1991-1994].	this study aims to describe and analyze the colonization of aedes albopictus whose presence was detected in 1991 in the area of sào josé do rio. preto already colonized by the aedes aegypti. year and month of occurrence, counties, composition and location of larval samples, kinds of containers, average number of larvae an breteau index bare been analyzed from the information obtained in measurements of larval density by superintendência de controle de endemias (sucen). the presence of aedes albo ...	1996	9011878
activity of novel juvenoids on arthropods of veterinary importance.	different juvenile hormone mimics were compared regarding their efficacy against larval and pupal stages of the diptera aedes aegypti, musca domestica, and lucilia cuprina as well as the cat flea ctenocephalides felis. structure-activity relationship of a series of new diphenylethers was analyzed and compared to juvenoids like methoprene, hydroprene, and fenoxycarb. selected compounds were taken for a competition binding assay and their effect on jh-degradation by esterases was examined. the res ...	1996	8756312
epidemic dengue 2 in the city of djibouti 1991-1992.	from october 1991 to february 1992, an outbreak of acute fever (in which thick blood films were negative for malaria) spread rapidly in the city of djibouti, djibouti republic, affecting all age groups and both nationals and foreigners. the estimated number of cases was 12,000. the clinical features were consistent with a non-haemorrhagic dengue-like illness. serum samples from 91 patients were analysed serologically for flavivirus infection (dengue 1-4, west nile, yellow fever, zika, banzi, and ...	1996	8758061
transovarial transmission of dengue 3 virus by aedes aegypti.		1996	9015501
laboratory evaluation of toxic baits for mosquito (aedes aegypti) larvae.	toxic bait discs were prepared by incorporating the larvicide abate (temephos) technical, with yeast and dog biscuit (1:1), the latter forming the attractant source. these were assessed in 1, 10 and 25 litre aquaria and 100 litre drums against aedes aegypti larvae. floating as well as sinking bait discs were found equally effective in controlling free moving larvae. larvae confined in artificially suspended cages were not affected. the baits were found effective for 5 to 6 weeks. toxic disc bait ...	1996	8781040
molecular characterization of the mosquito vitellogenin receptor reveals unexpected high homology to the drosophila yolk protein receptor.	the mosquito (aedes aegypti) vitellogenin receptor (aavgr) is a large membrane-bound protein (214 kda when linearized) that mediates internalization of vitellogenin, the major yolk-protein precursor, by oocytes during egg development. we have cloned and sequenced two cdna fragments encompassing the entire coding region of aavgr mrna, to our knowledge the first insect vgr sequence to be reported. the 7.3-kb aavgr mrna is present only in female germ-line cells and is abundant in previtellogenic oo ...	1996	8799131
bioefficacy of mosquito mat, coil and dispenser formulations containing allethrin group of synthetic pyrethroids against mosquito vectors.	the bioefficacy of mats, coils and dispensers containing allethrin group of synthetic pyrethroids was studied against laboratory strains of culex quinquefasciatus, aedes aegypti and anopheles stephensi. except esbiothrin in the mat formulation which was 100 and 178 times more effective against cx. quinquefasciatus than against ae. aegypti and an. stephensi with kt50 of 0.005, 0.5 and 0.89 min. respectively, all the three allethrins in mat or coil formulations were equally effective against the t ...	1996	8810142
effect of inactivation by sunlight on the larvicidal activities of mosquitocidal bacillus thuringiensis h-14 isolates from nigerian soils.	bacillus thuringiensis h-14 is a well recognized bioinsecticide against mosquitoes. this study investigates the inactivation effect of sunlight on the larvicidal activities of four nigerian isolates of b. thuringiensis h-14, code-named obg1, obg8, gsc3 and gna13 as compared with a standard mosquito larvicide, b. thuringiensis var. israelensis (bti). a 0.125 mg/1 suspension of spore-delta-endotoxin powder of each isolate was exposed to direct sunlight for 7 h on a concrete roof (ca. 13 m high). a ...	1996	8810143
evaluation of bacillus thuringiensis and bacillus sphaericus strains from chinese soils toxic to mosquito larvae	during our research to isolate and screen microbial agents, eight bacillus thuringiensis isolates and five bacillus sphaericus isolates were shown to have high toxicity to mosquito larvae. comparing the lc50 values, four b. sphaericus isolates with lc50 values ranging from 0.50 to 1.47 ng/ml were about two to six times more toxic than strain 1593 (lc50 3.00 ng/ml) against larvae of culex quinquefasciatus. four b. thuringiensis isolates (lc50 values ranging from 3.80 to 7.54 ng/ml) and four b. sp ...	1996	8812574
aedes aegypti: induced antibacterial proteins reduce the establishment and development of brugia malayi.	the effect of host immune activation on the development of brugia malayi in one susceptible and four refractory strains of aedes aegypti and in armigeres subalbatus was assessed. a. aegypti that were immune activated by the injection of saline or bacteria 24 hr before feeding on a b. malayi-infected gerbil had significantly reduced prevalences and mean intensities of infection from those of naive controls when exposed to bloodmeals with low (105 mf/20 microliters) and medium (160 mf/20 microlite ...	1996	8682188
characterization of the clinical and anatomical pathological changes associated with hepatozoon mocassini infections in unnatural reptilian hosts.	laboratory-reared aedes aegypti mosquitoes were employed in the successful transmission of hepatozoon mocassini from a cotton-mouth moccasin (agkistrodon piscivorus leucostoma) to 3 lizard species (sceloporus undulatus, eumeces obsoletus and sceloporus poinsetti). marked to severe lethargy and anorexia developed in the s. undulatus, e. obsoletus and s. poinsetti at 15, 38, and 96 days postinfection (pi), respectively. all 3 lizards developed a leukocytosis and had increased plasma aspartate amin ...	1996	8690537
evaluation of controlled-release mosquito repellent formulations.	eight polymer and 9 microcapsule formulations of deet were tested on laboratory rabbits against aedes aegypti and anopheles albimanus. several formulations were significantly more effective than simple (unformulated) deet at the same strength for periods up to 24 h. best results were obtained with a polymer formulation containing a high molecular weight fatty acid and 3 microcapsule formulations containing lanolin, gum arabic, gelatin, tannic acid, stearic acid, polypropylene glycol, water, and ...	1996	8723256
evaluation of the laboratory rabbit model for screening topical mosquito repellents.	the laboratory rabbit was evaluated as a model for screening topical mosquito repellents, using data obtained in tests of deet (n,n-diethyl-3-methylbenzamide) against aedes aegypti on humans and rabbits. host-specific differences in the action of the test material were quantified by multiple regression analysis. the test material was less effective but more persistent in tests on rabbits, and responses of the mosquito test population were more variable.	1996	8723273
lipid transfer from insect fat body to lipophorin: comparison between a mosquito triacylglycerol-rich lipophorin and a sphinx moth diacylglycerol-rich lipophorin.	two insect lipoproteins, triacylglycerol-rich aedes aegypti lipophorin and diacylglycerol-rich manduca sexta lipophorin, were compared in their ability to load neutral lipid from fat body. when fat body of m. sexta was incubated in vitro with [3h]oleic acid, all radiolabeled fatty acids were esterified, predominantly to triacylglycerol. in a. aegypti fat body, however, half of the label remained as free fatty acids. when a. aegypti fat body was radiolabeled with [3h]glycerol, most of the radiola ...	1996	8725165
surveillance of the mosquito aedes aegypti and its biocontrol with the copepod mesocyclops aspericornis in australian wells and gold mines.	a survey of the dengue vector mosquito aedes aegypti was undertaken using funnel traps to detect immature stages (larvae and pupae) in flooded disused mine shafts and wells in charters towers, queensland, northern australia. the town has a history of dengue fever since 1885 when goldminers were the first recorded victims. during the latest dengue epidemic in 1993, 2% of the population had laboratory-confirmed dengue virus type 2, despite source reduction of ae.aegypti breeding-sites at ground le ...	1996	8744708
antibody-mediated inhibition of aedes aegypti midgut trypsins blocks sporogonic development of plasmodium gallinaceum.	the peritrophic matrix (pm) that forms around a blood meal is a potential barrier of plasmodium development in mosquitoes. previously, we have shown that to traverse the pm, plasmodium ookinetes secrete a prochitinase and that an inhibitor of chitinase blocks further parasite development. here we report that it is the mosquito trypsin that activates the plasmodium prochitinase. trypsin was identified as the chitinase-activating enzyme by two criteria: (i) trypsin activity and activating activity ...	1996	8641775
cloning and sequencing of trehalose synthase gene from pimelobacter sp. r48.	the gene encoding trehalose synthase (catalyzing the conversion of maltose into alpha, alpha-trehalose by intramolecular transglucosylation) was cloned from pimelobacter sp. r48. sequence analysis revealed a 1719-bp synthase gene and a 573-residue amino-acid sequence. the 220 n-terminal residues were homologous to those of maltases from saccharomyces carlsbergensis and aedes aegypti.	1996	8645698
cloning and expression of the first anaerobic toxin gene from clostridium bifermentans subsp. malaysia, encoding a new mosquitocidal protein with homologies to bacillus thuringiensis delta-endotoxins.	a gene (cbm71) encoding a 71,128-da mosquitocidal protein (cbm71) was obtained by screening a size-fractionated xbai digest of total genomic dna from clostridium bifermentans subsp. malaysia ch18 with two gene-specific oligonucleotide probes. the sequence of the cbm71 protein, as deduced from the sequence of cbm71, corresponds to that of the 66-kda protein previously described as one of the mosquitocidal components of c. bifermentans subsp. malaysia. cbm71 shows limited similarities with bacillu ...	1996	8655486
experimental evidence against replication or dissemination of hepatitis c virus in mosquitoes (diptera:culicidae) using detection by reverse transcriptase polymerase chain reaction.	in 3 laboratory experiments, mosquitoes were fed hepatitis c virus (hcv)-rna positive blood by using membrane feeders, separated into head, thorax, and abdomen, and tested by a reverse transcriptase polymerase chain reaction for hcv-rna. hcv did not replicate or disseminate in mosquitoes that had ingested blood from patients that were hcv-viremic positive. when yellow fever mosquitoes, aedes aegypti (l.), were held for 1, 3, 7, 14, and 21 d after feeding, hcv-rna was detected in the abdomens of ...	1996	8667387
dengue vector mosquitos at a tourist attraction, ko samui, in 1995.	on ko samui, thailand there were two epidemics of dengue hemorrhagic fever (dhf) in 1966 and 1967, followed by endemics up to 1994. aedes aegypti and aedes albopictus were the vectors. from january to july 1995, 51 cases of dhf were reported, out of these were many foreigners who still suffer from dengue fever and return home with negative impression. we carried out an entomological survey around the island and collected the mosquitos to detect dengue virus by digoxigenin-cdna probe. the data re ...	1996	9031420
ultralow volume application of bacillus thuringiensis ssp. israelensis for the control of mosquitoes.	evaluation of the effectiveness of bacillus thuringiensis ssp. israelensis (b.t.i.) against mosquito larvae dispersed by ultralow volume (ulv) spraying was conducted in simulated field trials. effectiveness was measured using 3 different indicators: larval mortality, colony-forming unit enumeration, and droplet analysis. b.t.i. was dispersed with a ulv generator using 2 different flow rates: 0.3 and 0.5 liter/min on 2 different days. based on the results of this study, it can be concluded that a ...	1996	9046471
newsletters as a channel for communication in a community-based aedes aegypti control program in marília, brazil.	in marília, brazil, community newsletters were established in a pilot project on community-based aedes aegypti control. the newsletters were an excellent way of promoting communication between community members and project personnel. while people might not have attended a dengue meeting, they did turn up at meetings to plan the newsletters. during these meetings project staff obtained information about the communities that was crucial for planning a community-based recycling project that targete ...	1996	9046485
[detection of aedes aegypti and aedes albopictus, in an urban zone of the municipality of catanduva, sp, after control of a dengue epidemic].	after the realization of control research that had in view the transmission of dengue virus, we started to monitor two kinds of entomological vigilance, breteau index and ovitrap. we intended to evaluate the necessary time elapsed before aedes sp mosquitoes were again detected at the urban area of catanduva s town (sp). the ovitraps showed positiveness for the aedes aegypti two months after the control research, while the breteau index became positive only at the fourth month after the end of th ...	1996	9026829
domestic breeding sources and their contribution in anopheles stephensi breeding in dindigul, tamil nadu.	longitudinal study taken up for one year in 10 different types of breeding habitats in dindigul town, tamil nadu, revealed that out of 51,785 habitats 225 (0.43%) were found positive for anopheles stephensi immatures. the overall positivity varied between 0.03 to 1.31% with peak density during july. the observed habitat-wise positivity was overhead tanks 0-7.07%; wells 0-1.69%; under ground tanks 0-2.26%; tappits 0-2.36%; outside tanks (permanent) 0-2.42%; outside tanks (temporary) 0-0.39%; insi ...	1996	9125833
characterization and biological activity of a brazilian isolate of bacillus sphaericus (neide) highly toxic to mosquito larvae.	primary powders of bacillus sphaericus strain s2 isolated from soil samples in brazil, and strain 2362 were produced in a 14 liter fermentor. growth patterns and sporulation observed in three trials with strains s2 and 2362 in the fermentor were similar. second-instar larvae of culex quinquefasciatus, anopheles albimanus, anopheles quadrimaculatus, and aedes aegypti exposed for 48 hr to strain s2 responded with lc50 values of 0.25, 5.95, 12.28 and 140.0 ppb of lyophilized primary powder, respect ...	1996	9283664
characterization of six highly mosquitocidal bacillus thuringiensis strains that do not belong to h-14 serotype.	four strains belonging to bacillus thuringiensis serovars thompsoni, malaysiensis, canadensis, jegathesan and two auto-agglutinating b.t. strains were identified as being highly toxic to the mosquito larvae of the species aedes aegypti, anopheles stephensi, and culex pipiens. their larvicidal and hemolytic activities were determined and compared with those of strains known to be highly mosquitocidal and/or cytolytic from serovars of b.t. israelensis, morrisoni, darmstadiensis, medellin, kyushuen ...	1996	8555944
mosquitoes, models, and dengue.	in the last 10 years dengue has spread markedly through latin america and the caribbean (dominican republic, jamaica, barbados, mexico, guatemala, el salvador, honduras, nicaragua, costa rica, panama, ecuador, colombia, venezuela, and brazil). the mosquito aedes aegypti has taken advantage of increased urbanization and crowding to transmit the dengue virus. the mosquito infests tires, cans, and water jars near dwellings. the female mosquito practices multiple, interrupted feeding. thus, mosq ...	1996	8622446
possible contributing factors to the paucity of yellow fever epidemics in the ashanti region of ghana, west africa.	yellow fever virus vectors identified in the ashanti region of ghana included aedes aegypti, aedes africanus, aedes luteocephalus and aedes vittatus. other mosquito species, unrelated to yellow fever transmission, identified in this study included culex tigripes, culex thalassius, culex decens, culex tarsalis, anopheles gambiae, anopheles stephansi and toxorynchites brevipalpis. factors generally known to influence yellow fever transmission were also studied in the ashanti region. these included ...	1996	8625858
population genetics with rapd-pcr markers: the breeding structure of aedes aegypti in puerto rico.	rapd-pcr polymorphisms at 57 presumptive loci were used to examine the breeding structure of the mosquito aedes aegypti in puerto rico. mosquitoes were sampled from 16 locations in six cities and samples were located in a nested spatial design to examine local patterns of gene flow. allele frequencies were estimated assuming (1) that genomic regions amplified by rapd-pcr segregate as dominant alleles, (2) that genotypes at rapd loci are in hardy-weinberg proportions, (3) identity in state (iis) ...	1996	8626220
genetically engineered resistance to dengue-2 virus transmission in mosquitoes.	the control of arthropod-borne virus diseases such as dengue may ultimately require the genetic manipulation of mosquito vectors to disrupt virus transmission to human populations. to reduce the ability of mosquitoes to transmit dengue viruses, a recombinant sindbis virus was used to transduce female aedes aegypti with a 567-base antisense rna targeted to the premembrane coding region of dengue type 2 (den-2) virus. the transduced mosquitoes were unable to support replication of den-2 virus in t ...	1996	8629025
aedes aegypti midgut early trypsin is post-transcriptionally regulated by blood feeding.	early trypsin is a female-specific protease present in the aedes aegypti midgut during the first hours after ingestion of a blood meal. early trypsin gene expression was studied by northern blot analysis. the early trypsin mrna, absent in larvae, pupae and newly emerged females, reaches detectable levels at 24 h post-emergence and attains a maximum level at an adult age of 4-7 days. after the first week there is a decrease in the steady-state level of the transcript, but it remains readily detec ...	1996	8630532
mosquitocidal activity of the cryic delta-endotoxin from bacillus thuringiensis subsp. aizawai.	the cloned 135-kda cryic delta-endotoxin from bacillus thuringiensis is a lepidopteran-active toxin, displaying high activity in vivo against spodoptera litoralis and spodoptera frugiperda larvae and in vitro against the s. frugiperda sf9 cell line. here, we report that the cryic delta-endotoxin cloned from b. thuringienesis subsp. aizawai hd-229 and expressed in an acrystalliferous b. thuringiensis strain is also toxic to aedes aegypti, anophles gambiae, and culex quinquefasciatus mosquito larv ...	1996	8593070
[insects know no border.].	one of the major consequences of the joined action of demographic pressure and evolution of human activities lies in enormous increase of transportation, both in frequence and speed; taking advantage of these facilities, some arthropods could increase their geographical distribution and create new health threats. several demonstrative examples are exposed in order to illustrate this anthropic dissemination of the vectors : the introduction of anopheles gambiae s.l. in mauritius island between 18 ...	1996	17292311
influence of the copepod mesocyclops longisetus (crustacea: cyclopidae) on the survival of vibrio cholerae o1 in fresh water.	in an experimental microcosm, an analysis was performed of the influence exerted by freshwater mesocyclops longisetus copepods on the survival of vibrio cholerae o1 serovar inaba. in the state of ceará, copepods are used in the control of aedes aegypti larvae. the system consisted of water with a salinity of 0.27 per 10,000 habitants and ph 7.5, which after sterilizing filtration was distributed into seven flasks with a volume of 400 ml; in each of six flasks, 10 live copepods were inoculated al ...	1996	10904360
site-directed mutagenesis of an acetylcholinesterase gene from the yellow fever mosquito aedes aegypti confers insecticide insensitivity.	insecticide resistance is a serious problem facing the effective control of insect vectors of disease. insensitive acetylcholinesterase (ache) confers resistance to organophosphorus (op) and carbamate insecticides and is a widespread resistance mechanism in vector mosquitoes. although the point mutations that underlie ache insensitivity have been described from drosophila, the colorado potato beetle, and house flies, no resistance associated mutations have been documented from mosquitoes to date ...	1997	9371089
selected lines of aedes aegypti with persistently distorted sex ratios.	a breeding scheme to isolate x chromosomes sensitive to drive by the t8 (trinidad) y chromosome of aedes aegypti (the md haplotype) is reported. crosses with an australian strain th.i (thursday island) revealed not only sensitive and resistant x chromosomes but also some with the capacity to drive against the t8 y chromosome. four strains were created in which sex ratio was male-distorted (28-36 per cent female) for 10 generations, with no regression towards sexual parity. the proportion of fema ...	1997	9353868
oviposition responses of aedes aegypti and ae. atropalpus (diptera: culicidae) females to waters from conspecific and heterospecific normal larvae and from larvae infected with plagiorchis elegans (trematoda: plagiorchiidae).	ovipositing aedes aegypti (l.) females were attracted to waters in which uninfected aedes atropalpus (coquillett) larvae had been reared, but were repelled by waters from larvae of either species infected with the entomopathogenic digenean plagiorchis elegans (rudolphi). in contrast, ae. atropalpus females were attracted to or repelled by waters from conspecific uninfected and infected larvae, respectively, but did not respond to corresponding waters from ae. aegypti larvae. the sensitivity of o ...	1997	9379463
isolation and properties of drosophila melanogaster ferritin--molecular cloning of a cdna that encodes one subunit, and localization of the gene on the third chromosome.	ferritin was purified from iron-fed drosophila melanogaster extracts by centrifugation in a gradient of potassium bromide. on polyacrylamide gel electrophoresis, the product showed two protein bands corresponding to the ferritin monomer and dimer. electrophoresis following dissociation with sds and 2-mercaptoethanol revealed three strong bands of approximately 25, 26, and 28 kda. n-terminal amino acid sequences were identical for the 25-kda and 26-kda subunits, but different for the 28-kda subun ...	1997	9266686
effect of ebastine on mosquito bites.	mosquito bites usually cause wealing and delayed bite papules. cetirizine decreases wealing, bite papules and pruritus but the effect of other antihistamines on mosquito bites is unknown. we studied the effect of ebastine in 30 mosquito bite-sensitive adult subjects. ebastine 10 mg or 20 mg and placebo were given for 4 days in a cross-over fashion. aedes aegypti bites were given on forearms. the size of the bite lesions and pruritus (visual analogue score) were measured at 15 min, 2, 6, and 24 h ...	1997	9228228
reduced transfer of male accessory gland proteins and monandry in female aedes aegypti mosquitoes.	we examined whether female aedes aegypti (l.) mosquitoes would remate after they first mated with a male that was reared on a suboptimal larval diet and that as a result, transferred reduced amounts of male accessory gland proteins. accessory gland proteins from males labeled with 3h leucine were not detected in females allowed to male mate with the labeled males after they first mated with either low diet or high diet males. the amount of the male accessory gland protein transferred by smaller, ...	1997	9221745
zebedee: a novel copia-ty1 family of transposable elements in the genome of the medically important mosquito aedes aegypti.	we have utilised pcr to directly identify a novel family of copia-ty1 retrotransposable elements (rtps) in the genome of the mosquito aedes aegypti. two members of the family have been sequenced in their entirety and their structural characteristics determined. zebedeei is 3505 bp long and appears to be flanked by 21bp direct repeat sequences. a single open reading frame (orf) of 972 amino acids has the coding potential for a polyprotein with homology corresponding to the conserved amino acid mo ...	1997	9197409
transposition of the hermes element in embryos of the vector mosquito, aedes aegypti.	using a plasmid-based transpositional recombination assay in vivo, we have demonstrated that hermes, a short inverted repeat type transposable element from musca domestica, can transpose in aedes aegypti embryos. hermes transpositions in ae. aegypti have all the characteristics observed during hermes transposition in its host m. domestica and in related species. these characteristics include an absolute dependence on the expression of the hermes transposase and a preference for the integration s ...	1997	9219363
molecular characterization of a defensin gene from the mosquito, aedes aegypti.	insect immune proteins, defensins, are inducible anti-gram-positive bacterial peptides. we report here the identification of two defensin genes from the mosquito, aedes aegypti, which encode a large 541 bp transcript (aadef ala) and a small 473 bp transcript (aadef asm). the cdna corresponding to aadef ala was cloned, sequenced, and compared with the previously reported aadef asm cdna. the aadef ala gene was isolated through genomic library screening and characterized. it putative regulatory reg ...	1997	9219362
fish landmarks for aedes aegypti chromosomes.	aedes aegypti metaphase chromosome landmarks have been developed so that each chromosome of the haploid genome can be unambiguously identified and oriented by fluorescence in situ hybridization (fish) and digital imaging microscopy. the fish tags were derived from three cosmids that contain repetitive ae. aegypti sequences and their unique fish tagging characteristics are demonstrated. the sequence of the three chromosomal tags revealed that the chromosome 1 tag is an 18s fragment from the ribos ...	1997	9099584
insulin-mediated secretion of ecdysteroids from mosquito ovaries and molecular cloning of the insulin receptor homologue from ovaries of bloodfed aedes aegypti.	the reproductive cycle of female mosquitoes is activated by ingestion of blood from vertebrate hosts. shortly after feeding, neurohormones are released from the brain neurosecretory system and stimulate the ovaries to secrete ecdysteroids, which are necessary for vitellogenesis by the fat body. because bombyxins, which are insulin-like peptides, stimulate ecdysteriodgenesis in silkworm larvae, we tested porcine insulin and found that it activates ecdysteroidogenesis and protein synthesis in ovar ...	1997	9099579
a single cdna encodes all three aedes leucokinins, which stimulate both fluid secretion by the malpighian tubules and hindgut contractions.	a cdna encoding preproleucokinin was isolated from a cdna library of the mosquito aedes aegypti. the deduced amino acid sequence of aedes preproleucokinin contains a putative signal peptide of 18 amino acid residues and a 210-amino acid residue proleucokinin. within the proleucokinin are encoded one copy each of the aedes leucokinins 1, 2, and 3 isolated previously from this species (veenstra, j. a. (1994) biochem. biophys. res. commun. 202, 715-719). all three aedes leucokinins depolarize the t ...	1997	9099680
inheritance of larval resistance to permethrin in aedes aegypti and association with sex ratio distortion and life history variation.	the genetic mechanisms that confer larval permethrin resistance were investigated in two strains of aedes aegypti, vectors of yellow fever and dengue hemorrhagic fever. larval resistance to permethrin in an ae. aegypti field-collected resistant couva (r) strain was associated with the sex-determining locus by analysis of backcrosses to the susceptible rockefeller (s) strain. the median lethal concentrations (lc50s) of these strains were 23.1 (95% confidence interval = 22.0-24.3) and 2.2 (2.0-2.3 ...	1997	9158058
cdna cloning and pattern of expression of an adult, female-specific chymotrypsin from aedes aegypti midgut.	a cdna for a midgut chymotrypsin, induced by a blood meal, has been cloned and sequenced from the mosquito aedes aegypti. the 938 base sequence codes for a 268 amino acid protein, which contains an 18-residue signal peptide and a seven-residue activation peptide. the deduced amino acid sequence contains several features typical of chymotrypsin proteases, including the catalytic triad of serine proteases and the residues that determine the chymotrypsin substrate specificity pocket. the chymotryps ...	1997	9134710
aedes aegypti surveillance and correlation with the occurrence of dengue fever in bahia, brazil.	due to the high frequency of dengue fever cases and the presumed association of such an epidemic with an increase in the population of the mosquito vector, aedes aegypti, we examined the records of the ministry of health in the state of bahia, brazil, regarding the monitoring of domestic mosquito larvae in municipalities throughout the state. the "house index" number for larvae in domestic water reservoirs was determined for each municipality based on annual surveys from 1990 to 1994, and in 199 ...	1997	11107237
effects of two insect growth regulators on the susceptibility of aedes aegypti (diptera:culicidae) to molinema dessetae (nematoda:filarioidea).	the effects of 2 growth regulators, diflubenzuron (dfb) and oms 2017, on the ability of females aedes aegypti to become infected with molinema dessetae was studied under laboratory conditions. oms 2017 and dfb had no effect on either the amount of blood ingested or the microfilarial load. the infective potential of females that survived dfb treatment was significantly greater than untreated females, but there was no difference between oms 2017-treated and control females. the percentage of infec ...	1997	9152874
methoprene affects the rotation of the male terminalia of aedes aegypti mosquitoes.	the rotation of the male terminalia of aedes aegypti mosquitoes was investigated with chemical surgical, and mechanical methods. neither decapitation nor ventral nerve cord transection affected the postemergence rotation, but methoprene administered to 4th-instar larvae and early instar pupae slowed rotation significantly and, in the case of pupal treatment, also interfered with successful insemination. methoprene appeared to interfere with the developmental maturation of the cross muscles locat ...	1997	9152867
biochemical, molecular, and phylogenetic analysis of pyruvate carboxylase in the yellow fever mosquito, aedes aegypti.	pyruvate carboxylase (pc, pyruvate: carbon dioxide ligase [adp-forming], ec 6.4.1.1) was purified from the yellow fever mosquito, aedes aegypti. the purified pc showed two polypeptides of similar m(r) (133 and 128 k). the n-terminal sequences of both polypeptides were shown to be very similar, if not identical. a polyclonal antiserum against the 133 kda polypeptide cross-reacted strongly with the 128 kda polypeptide. pc was found in all tissues examined. using a semi-quantitative western blot as ...	1997	9066123
juvenile hormone controls early trypsin gene transcription in the midgut of aedes aegypti.	early trypsin is a female-specific protease present in the aedes aegypti midgut during the first few hours after ingestion of a blood meal. the enzymatic activity of early trypsin plays an essential role in the transcriptional activation of the late trypsin gene, which encodes the major midgut endoprotease involved in blood meal protein digestion. transcription of the early trypsin gene is part of the normal post-emergence maturation of the midgut in the adult female. abdominal ligation within 1 ...	1997	9013256
identification of three allatostatins and their cdna from the mosquito aedes aegypti.	three allatostatins have been isolated from the mosquito aedes aegypti. these peptides have the following structures: ser-pro-lys-tyr-asn-phc-gly-leu-amide, leu-pro-his-tyr-asn-phe-gly-leu-amide, and arg-val-tyr-asp-phe-gly-leu-amide. a cdna encoding these peptides was isolated from an abdominal ganglia cdna library and sequenced. it was found to encode two additional allatostatins: ala-ser-ala-tyr-arg-tyr-his-phe-gly-leu-amide and leu-pro-asn-arg-tyr-asn-phe-gly-leu-amide. northern analysis of ...	1997	9357049
structure of the gypsy moth vitellogenin gene.	genomic clones containing the vitellogenin (vg) gene from the gypsy moth were isolated from two genomic libraries and characterized. the nucleotide sequence of a 16,132 bp region of the gypsy moth genome was determined which included a 3,666 bp region upstream from the transcription initiation site and 499 bp region downstream from the transcribed region. primer extension analysis was performed to identify the transcription initiation site. gene sequence confirmed the sequence of vgmrna recently ...	1997	9327584
central projections of the maxillary and antennal nerves in the mosquito aedes aegypti	in the mosquito aedes aegypti, co2-sensitive receptor neurones are located together with two other types of chemoreceptor neurones in club-shaped sensilla basiconica on the most distal segment of the maxillary palps. in order to identify the central target neuropiles of these neurones and to determine whether antennal receptor neurones project into the same area, the palpal and antennal nerves were labelled by anterograde staining with horseradish peroxidase and by experimentally induced degener ...	1997	9319784
the feasibility of eradicating aedes aegypti in the americas.		1997	9128110
lipophorin levels in the yellow fever mosquito, aedes aegypti, and the effect of feeding.	high density lipophorin (hdlp) is the major lipid transport vehicle in insect hemolymph. using an indirect elisa, levels of hdlp were measured in the yellow fever mosquito, aedes aegypti. the level of lipophorin, when normalized to the total weight of the insect, was similar in the different developmental stages. starvation (access to water only) of adult females did not affect the level of hdlp nor its density when compared to sugar-fed females. on the other hand, blood feeding (of normally sug ...	1997	9055439
the gecko: an environmentally friendly biological agent for mosquito control.	laboratory experiments with aedes aegypti mosquitoes investigated the effects of light, mosquito density and physiological state on predation rates by the australian gecko gehydra dubia and the exotic asian house gecko hemidactylus frenatus. for both gecko species a positive correlation was demonstrated between prey density and the predation rate. using ae.aegypti males and unfed females as prey in a terrarium (0.054 m3), consumption rates reached 76-108/day for g.dubia and 63-109/day for h.fren ...	1997	9430109
preliminary field evaluation of the combined adulticidal, larvicidal, and wall residual activity of ulv-applied bifenthrin against mosquitoes.	the combined adulticidal, larvicidal, and wall residual activity of ulv-applied bifenthrin, a synthetic pyrethroid, was evaluated in houses in kuala lumpur, malaysia, against larvae and adults of lab-bred aedes aegypti and culex quinquefasciatus. a portable ulv sprayer was used to disperse a ulv formulation of bifenthrin at a discharge rate of 45 ml/min. the results indicated that bifenthrin sprayed at this rate exhibited all the three activities against the test mosquitoes. complete adult morta ...	1997	9491365
transamination of 3-hydroxykynurenine to produce xanthurenic acid: a major branch pathway of tryptophan metabolism in the mosquito, aedes aegypti, during larval development.	an electrochemically active compound was detected in the larvae of aedes aegypti mosquitoes and progressive accumulation of this compound was observed during larval development. the compound was purified from mosquito larvae using various chromatographic techniques and spectral analysis of the purified compound resulted in its identification as xanthurenic acid. production of xanthurenic acid results from the transamination of 3-hydroxykynuorenine, and analysis of the biochemical pathway in xant ...	1997	9474782
an equipment for the study of behavioural responses of mosquitoes to residual application of synthetic insecticides.	excito-repellency (e-r) test box was developed to study under laboratory conditions the refractory behaviour of mosquitoes to residual application of synthetic insecticides. the observations obtained through the equipment were compared with another behavioural study of mosquitoes using standard who irritability test kit. during 16 hour exposure period there was restricted entry of mosquitoes in ddt treated chamber of e-r test box for all mosquito species tested (anopheles stephensi, aedes aegypt ...	1997	9465527
laboratory evaluation of the biocontrol potential of mesocyclops thermocyclopoides (copepoda: cyclopidae) against mosquito larvae.	biocontrol potential of mesocyclops thermocyclopoides against first instar larvae of anopheles stephensi, aedes aegypti and culex quinquefasciatus was studied under laboratory conditions. it was found that m. thermocyclopoides had the highest predation efficacy against ae. aegypti followed by an. stephensi and cx. quinquefasciatus. there was a significant reduction in the predation efficacy of m. thermocyclopoides against cx. quinquefasciatus in the presence of alternate food (p < 0.01). the cag ...	1997	9656415
cloning of cdna for vitellogenin of the parasitoid wasp, pimpla nipponica (hymenoptera: apocrita: ichneumonidae): vitellogenin primary structure and evolutionary considerations.	the cdna for vitellogenin (vg) of the parasitoid wasp pimpla nipponica (hymenoptera: apocrita) was cloned and sequenced. the deduced amino acid sequence with 1807 residues was obtained. the n-terminal 20 amino acids chemically determined for vitellin (vn) agreed completely with the deduced 20 amino acids that follow the 16 amino acid residues for putative signal peptide. the cdna clone for the vg of the turnip sawfly athalia rosae (hymenoptera: symphyta), previously obtained and partially sequen ...	1997	9569645
transient expression of the drosophila melanogaster cinnabar gene rescues eye color in the white eye (we) strain of aedes aegypti.	the lack of eye pigment in the aedes aegypti we (white eye) colony was confirmed to be due to a mutation in the kynurenine hydroxylase gene, which catalyzes one of the steps in the metabolic synthesis of ommochrome eye pigments. partial restoration of eye color (orange to red phenotype) in pupae and adults occurred in both sexes when first or second instar larvae were reared in water containing 3-hydroxykynurenine, the metabolic product of the enzyme kynurenine hydroxylase. no eye color restorat ...	1997	9569641
costs and benefits of mosquito refractoriness to malaria parasites: implications for genetic variability of mosquitoes and genetic control of malaria.	the problem of fitness costs associated with host resistance to parasitism is related to the evolution of parasite virulence, population genetic diversity and the dynamics of host-parasite relationships, and proposed strategies for disease control through the genetic manipulation of mosquito vectors. two aedes aegypti populations, refractory and susceptible to plasmodium gallinaceum, were previously selected from the moyo-in-dry strain (moyo) through inbreeding (f = 0.5). reproductive success an ...	1997	28565348
natriuretic and depolarizing effects of a stable fly (stomoxys calcitrans) factor on malpighian tubules.	a two-step hplc purification procedure resulted in a factor from the stable fly that depolarizes the lumen-negative transepithelial voltage (v(t)) of the adult stable fly malpighian tubule. when applied to tubules of the female mosquito, aedes aegypti, this factor partially mimics the electrophysiological actions of the mosquito natriuretic factor (mnf). it also selectively increases active transepithelial na transport by the mosquito malpighian tubule. the blood meal causes a transient increase ...	1997	12770470
impact of dengue virus infection on feeding behavior of aedes aegypti.	in addition to heavily infecting the salivary glands of aedes aegypti (l.) mosquitoes, dengue viruses produce a significant infection of the nervous system, involving the brain, johnston's organ, compound eye, and thoracic and abdominal ganglion. to determine if dengue infection affects feeding behavior of ae. aegypti we measured feeding times, counted the number of feeding delays or interruptions, and by in situ immunocytochemistry techniques determined the spatial and temporal distribution of ...	1997	9288801
venereal and vertical transmission of the aedes albopictus parvovirus in aedes aegypti mosquitoes.	following per oral infection of aedes aegypti larvae with aedes albopictus parvovirus (aapv), infected males and females adults were tested for their ability to transmit the virus venereally and vertically, respectively. both types of transmission were observed. a low percentage (2.2%) of aapv-free females were found contaminated by the virus after mating with aapv-infected males. although no significant difference was observed in the fecundity of orally infected and virus-free females, 17.1% of ...	1997	9288802